Top1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Top1
Entrez Gene ID	64550
Full Name	DNA topoisomerase I
Synonyms	Ab2-086
General protein information	Preferred Names DNA topoisomerase I Names DNA topoisomerase 1 topoisomerase (DNA) I
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42
Summary	human homolog catalyzes the transient breakage and rejoining of DNA; plays a role in nucleotide excision repair [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022615.1	NP_072137.1	DNA topoisomerase 1

Pathways from BioSystems

Homologies

	TOP1	XP_001088021.1
Bos taurus (cattle)	TOP1	NP_001193416.1
Gallus gallus (chicken)	TOP1	NP_990441.1
Neurospora crassa	NCU09118	XP_958033.2
Arabidopsis thaliana (thale cress)	TOP1ALPHA	NP_200341.1
Canis lupus familiaris (dog)	TOP1	XP_534420.3
Caenorhabditis elegans (roundworm)	top-1	NP_001021578.1
Saccharomyces cerevisiae (baker's yeast)	TOP1	NP_014637.1
Magnaporthe oryzae (rice blast fungus)	MGG_09917	XP_003710015.1
Arabidopsis thaliana (thale cress)	TOP1BETA	NP_200342.1
Kluyveromyces lactis	KLLA0C06116g	XP_452470.1
Eremothecium gossypii	AGOS_ACL008C	NP_983396.2
Xenopus tropicalis (tropical clawed frog)	LOC100496521	XP_002932918.2
Mus musculus (house mouse)	Top1	NP_033434.2
Danio rerio (zebrafish)	top1l	NP_001037789.1
Oryza sativa (rice)	Os08g0154600	NP_001061015.1
Homo sapiens (human)	TOP1	NP_003277.1
Pan troglodytes (chimpanzee)	TOP1	XP_514649.2
Rattus norvegicus (Norway rat)	Top1	NP_072137.1
Drosophila melanogaster (fruit fly)	Top1	NP_001014742.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004078	XP_003436707.1
Schizosaccharomyces pombe (fission yeast)	top1	NP_596209.1

Gene Ontology
Bibliography

Related articles in PubMed

Rac1 protein signaling is required for DNA damage response stimulated by topoisomerase II poisons.
Huelsenbeck SC, Schorr A, Roos WP, Huelsenbeck J, Henninger C, Kaina B, Fritz G
The Journal of biological chemistry287(46)38590-9(2012 Nov)

Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period.
Onishi Y, Kawano Y
Nucleic acids research40(19)9482-92(2012 Oct)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

The giant fibrillar center: a nucleolar structure enriched in upstream binding factor (UBF) that appears in transcriptionally more active sensory ganglia neurons.
Casafont I, Bengoechea R, Navascu?s J, Pena E, Berciano MT, Lafarga M
Journal of structural biology159(3)451-61(2007 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rac1-regulated mechanisms are required for DNA damage induction and subsequent activation of the DDR following treatment with topo II but not topo I poisons
Title: Rac1 protein signaling is required for DNA damage response stimulated by topoisomerase II poisons.

The following Top1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Top1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13464	NM_022615.1 Latest version!	Rattus norvegicus DNA topoisomerase I (Top1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$454.30 $649.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Top1 ( NM_022615.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072137.1 Rattus norvegicus DNA topoisomerase I (Top1), mRNA.

Price & Availability↑

CloneID	ORa13464
Clone ID Related Accession (Same CDS sequence)	NM_022615.1
Accession Version	NM_022615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF140782.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF140782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGCGGGG ACCATCTCCA CAACGACTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCATAAGGA GCACAAGAAG 
GATAAGGATA AGGACCGGGA AAAGTCTAAA CATAGCAACA GTGAACATAA AGATTCTGAA 
AAGAAACACA AAGAGAAAGA GAAAACCAAA CACAAAGATG GAAGCTCAGA CAAACATAAA 
GACAAACATA AAGACAGAGA CAAGGAAAAA CGAAAGGAGG AAAAGATTAG AGCTGCTGGG 
GATGCAAAAA TAAAGAAGGA GAAGGAAAAT GGCTTCTCTA GTCCACCACG AATTAAAGAC 
GAGCCTGAAG ATGATGGCTA TTTTGCTCCT CCAAAAGAGG ATATAAAGCC ACTGAAGAGG 
CCTCGGGATG AAGATGATGC TGATTATAAG CCTAAGAAAA TTAAGACAGA AGATATCAAG 
AAGGAGAAGA AACGAAAACT AGAAGAAGAA GAGGATGGCA AACTTAAAAA GCCCAAGAAT 
AAAGATAAAG ATAAAAAGGT TGCTGAGCCA GATAATAAGA AGAAGAAGGC GAAAAAGGAA 
GAGGAGCAGA AGTGGAAATG GTGGGAAGAA GAACGCTATC CAGAAGGCAT CAAGTGGAAA 
TTCCTAGAAC ATAAAGGGCC TGTTTTCGCT CCACCGTATG AGCCTCTGCC AGAGGGTGTC 
AAGTTTTACT ATGATGGTAA AGTTATGAAG CTGAGTCCCA AAGCAGAAGA AGTAGCTACA 
TTCTTTGCAA AAATGCTTGA CCATGAATAT ACTACTAAGG AAATATTCAG GAAAAATTTC 
TTTAAAGACT GGAGAAAGGA AATGACTAAT GATGAAAAAA ATACGATTAC CAACCTAAGC 
AAATGCGACT TTACACAGAT GAGCCAGTAC TTCAAAGCTC AATCAGAAGC TCGGAAACAG 
ATGAGCAAGG AAGAAAAATT GAAAATCAAA GAAGAAAATG AAAAATTATT GAAAGAATAT 
GGCTTTTGTG TAATGGATAA TCATAGAGAG CGGATTGCCA ACTTCAAGAT AGAGCCTCCA 
GGGCTCTTCC GAGGCCGTGG CAACCACCCC AAGATGGGTA TGCTGAAGAG AAGGATCATG 
CCTGAGGACA TCATCATCAA CTGTAGCAAA GATGCCAAGG TTCCTTCTCC TCCTCCTGGA 
CATAAATGGA AGGAAGTTCG ACATGATAAC AAGGTTACTT GGCTGGTCTC CTGGACAGAG 
AATATCCAAG GTTCTATCAA ATATATCATG CTGAATCCCA GTTCACGAAT CAAGGGTGAG 
AAAGACTGGC AGAAGTATGA GACTGCTCGG CGACTGAAGA AGTGTGTGGA CAAGATCCGA 
AATCAGTATC GGGAAGACTG GAAGTCCAAA GAAATGAAAG TTCGGCAGAG AGCTGTAGCA 
CTGTACTTCA TTGATAAGCT TGCTCTGAGA GCAGGCAATG AGAAGGAAGA AGGAGAGACA 
GCAGACACTG TGGGTTGCTG TTCACTTAGA GTGGAACACA TCAATCTACA CCCAGAATTG 
GATGGTCAGG AGTACGTGGT GGAGTTTGAC TTTCCTGGGA AGGACTCAAT CAGATACTAC 
AACAAAGTCC CAGTTGAGAA ACGAGTTTTT AAAAACTTAC AACTATTTAT GGAGAACAAG 
CAGCCTGAGG ATGATCTTTT TGATCGACTT AATACTGGTA TTCTAAATAA GCATCTTCAA 
GATCTCATGG AGGGCTTGAC TGCCAAGGTG TTCCGTACCT ACAATGCCTC CATCACGCTG 
CAGCAGCAGC TTAAAGAGCT CACAGCCCCT GATGAGAATG TACCAGCAAA GATTCTATCT 
TATAACCGTG CCAATCGCGC TGTTGCAATT CTTTGTAACC ACCAGAGGGC GCCACCAAAG 
ACCTTTGAGA AGTCAATGAT GAACTTGCAG TCTAAGATTG ATGCCAAGAA AGATCAGCTA 
GCAGATGCTC GAAAGGACCT GAAAAGCGCT AAGGCTGATG CCAAAGTCAT GAAGGATGCA 
AAGACCAAGA AGGTAGTAGA GTCAAAAAAG AAGGCTGTAC AGAGATTAGA AGAGCAGCTG 
ATGAAGCTGG AGGTTCAAGC CACAGACCGA GAGGAGAACA AACAAATTGC CTTGGGGACC 
TCCAAACTCA ATTACCTGGA CCCTAGGATC ACAGTGGCTT GGTGCAAAAA ATGGGGGGTC 
CCAATCGAGA AGATTTACAA CAAAACCCAG AGAGAGAAGT TTGCTTGGGC CATTGATATG 
ACTGATGAGG ACTACGAGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072137.1
CDS	1..2304
Translation	MSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKDKDKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSDKHKDKHKDRDKEKRKEEKIRAAGDAKIKKEKENGFSSPPRIKDEPEDDGYFAPPKEDIKPLKRPRDEDDADYKPKKIKTEDIKKEKKRKLEEEEDGKLKKPKNKDKDKKVAEPDNKKKKAKKEEEQKWKWWEEERYPEGIKWKFLEHKGPVFAPPYEPLPEGVKFYYDGKVMKLSPKAEEVATFFAKMLDHEYTTKEIFRKNFFKDWRKEMTNDEKNTITNLSKCDFTQMSQYFKAQSEARKQMSKEEKLKIKEENEKLLKEYGFCVMDNHRERIANFKIEPPGLFRGRGNHPKMGMLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNPSSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFPGKDSIRYYNKVPVEKRVFKNLQLFMENKQPEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDENVPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQSKIDAKKDQLADARKDLKSAKADAKVMKDAKTKKVVESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKIYNKTQREKFAWAIDMTDEDYEF

Target ORF information:

RefSeq Version	NM_022615.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus DNA topoisomerase I (Top1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022615.1

ORF Insert Sequence:

ATGAGCGGGG ACCATCTCCA CAACGACTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCATAAGGA GCACAAGAAG 
GATAAGGATA AGGACCGGGA AAAGTCTAAA CATAGCAACA GTGAACATAA AGATTCTGAA 
AAGAAACACA AAGAGAAAGA GAAAACCAAA CACAAAGATG GAAGCTCAGA CAAACATAAA 
GACAAACATA AAGACAGAGA CAAGGAAAAA CGAAAGGAGG AAAAGATTAG AGCTGCTGGG 
GATGCAAAAA TAAAGAAGGA GAAGGAAAAT GGCTTCTCTA GTCCACCACG AATTAAAGAC 
GAGCCTGAAG ATGATGGCTA TTTTGCTCCT CCAAAAGAGG ATATAAAGCC ACTGAAGAGG 
CCTCGGGATG AAGATGATGC TGATTATAAG CCTAAGAAAA TTAAGACAGA AGATATCAAG 
AAGGAGAAGA AACGAAAACT AGAAGAAGAA GAGGATGGCA AACTTAAAAA GCCCAAGAAT 
AAAGATAAAG ATAAAAAGGT TGCTGAGCCA GATAATAAGA AGAAGAAGGC GAAAAAGGAA 
GAGGAGCAGA AGTGGAAATG GTGGGAAGAA GAACGCTATC CAGAAGGCAT CAAGTGGAAA 
TTCCTAGAAC ATAAAGGGCC TGTTTTCGCT CCACCGTATG AGCCTCTGCC AGAGGGTGTC 
AAGTTTTACT ATGATGGTAA AGTTATGAAG CTGAGTCCCA AAGCAGAAGA AGTAGCTACA 
TTCTTTGCAA AAATGCTTGA CCATGAATAT ACTACTAAGG AAATATTCAG GAAAAATTTC 
TTTAAAGACT GGAGAAAGGA AATGACTAAT GATGAAAAAA ATACGATTAC CAACCTAAGC 
AAATGCGACT TTACACAGAT GAGCCAGTAC TTCAAAGCTC AATCAGAAGC TCGGAAACAG 
ATGAGCAAGG AAGAAAAATT GAAAATCAAA GAAGAAAATG AAAAATTATT GAAAGAATAT 
GGCTTTTGTG TAATGGATAA TCATAGAGAG CGGATTGCCA ACTTCAAGAT AGAGCCTCCA 
GGGCTCTTCC GAGGCCGTGG CAACCACCCC AAGATGGGTA TGCTGAAGAG AAGGATCATG 
CCTGAGGACA TCATCATCAA CTGTAGCAAA GATGCCAAGG TTCCTTCTCC TCCTCCTGGA 
CATAAATGGA AGGAAGTTCG ACATGATAAC AAGGTTACTT GGCTGGTCTC CTGGACAGAG 
AATATCCAAG GTTCTATCAA ATATATCATG CTGAATCCCA GTTCACGAAT CAAGGGTGAG 
AAAGACTGGC AGAAGTATGA GACTGCTCGG CGACTGAAGA AGTGTGTGGA CAAGATCCGA 
AATCAGTATC GGGAAGACTG GAAGTCCAAA GAAATGAAAG TTCGGCAGAG AGCTGTAGCA 
CTGTACTTCA TTGATAAGCT TGCTCTGAGA GCAGGCAATG AGAAGGAAGA AGGAGAGACA 
GCAGACACTG TGGGTTGCTG TTCACTTAGA GTGGAACACA TCAATCTACA CCCAGAATTG 
GATGGTCAGG AGTACGTGGT GGAGTTTGAC TTTCCTGGGA AGGACTCAAT CAGATACTAC 
AACAAAGTCC CAGTTGAGAA ACGAGTTTTT AAAAACTTAC AACTATTTAT GGAGAACAAG 
CAGCCTGAGG ATGATCTTTT TGATCGACTT AATACTGGTA TTCTAAATAA GCATCTTCAA 
GATCTCATGG AGGGCTTGAC TGCCAAGGTG TTCCGTACCT ACAATGCCTC CATCACGCTG 
CAGCAGCAGC TTAAAGAGCT CACAGCCCCT GATGAGAATG TACCAGCAAA GATTCTATCT 
TATAACCGTG CCAATCGCGC TGTTGCAATT CTTTGTAACC ACCAGAGGGC GCCACCAAAG 
ACCTTTGAGA AGTCAATGAT GAACTTGCAG TCTAAGATTG ATGCCAAGAA AGATCAGCTA 
GCAGATGCTC GAAAGGACCT GAAAAGCGCT AAGGCTGATG CCAAAGTCAT GAAGGATGCA 
AAGACCAAGA AGGTAGTAGA GTCAAAAAAG AAGGCTGTAC AGAGATTAGA AGAGCAGCTG 
ATGAAGCTGG AGGTTCAAGC CACAGACCGA GAGGAGAACA AACAAATTGC CTTGGGGACC 
TCCAAACTCA ATTACCTGGA CCCTAGGATC ACAGTGGCTT GGTGCAAAAA ATGGGGGGTC 
CCAATCGAGA AGATTTACAA CAAAACCCAG AGAGAGAAGT TTGCTTGGGC CATTGATATG 
ACTGATGAGG ACTACGAGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rac1 protein signaling is required for DNA damage response stimulated by topoisomerase II poisons.
The Journal of biological chemistry287(46)38590-9(2012 Nov)
Huelsenbeck SC,Schorr A,Roos WP,Huelsenbeck J,Henninger C,Kaina B,Fritz G

Rhythmic binding of Topoisomerase I impacts on the transcription of Bmal1 and circadian period.
Nucleic acids research40(19)9482-92(2012 Oct)
Onishi Y,Kawano Y

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

The giant fibrillar center: a nucleolar structure enriched in upstream binding factor (UBF) that appears in transcriptionally more active sensory ganglia neurons.
Journal of structural biology159(3)451-61(2007 Sep)
Casafont I,Bengoechea R,Navascu?s J,Pena E,Berciano MT,Lafarga M