hps5 cDNA ORF clone, Poecilia reticulata(guppy)

Gene
Clones
gRNAs

Summary

Gene Symbol	hps5
Entrez Gene ID	103466493
Full Name	HPS5, biogenesis of lysosomal organelles complex 2 subunit 2
Gene Type	protein-coding
Organism	Poecilia reticulata(guppy)

mRNA and Protein(s)

mRNA	Protein	Name
XM_008412088.2	XP_008410310.1	Hermansky-Pudlak syndrome 5 protein
XM_008412089.2	XP_008410311.1	Hermansky-Pudlak syndrome 5 protein

Pathways from BioSystems
Gene Ontology

The following hps5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hps5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPo197298	XM_008412088.2 Latest version!	Poecilia reticulata HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 (hps5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo197298	XM_008412089.2 Latest version!	Poecilia reticulata HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 (hps5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hps5 ( XM_008412088.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008410310.1 Poecilia reticulata HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 (hps5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo197298
Clone ID Related Accession (Same CDS sequence)	XM_008412088.2 , XM_008412089.2
Accession Version	XM_008412088.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	Hermansky-Pudlak syndrome 5 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008412088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA TACCGGTTGT GCCTGAGAAC CACAGTCACC TCCTGGCAGA GTTCGACTGC 
CTGGATCCGC TCCTCTCTGC TCTGCGTTTT GACTCAGGCA GGCTGAAGTG TACCTGTCTG 
GCGGTGTCCA GGAAGTGGCT CGCGTTGGGA ACGTCAGCAG GAGGGTTGCA CCTCATCCAG 
AAGGAGGGCT GGAAACAGAG ACTCATTCTG ACTCACAAGG AGGGATCTAT CACACAAGTG 
GCATGTTGTC CTCATGATGA AGATTTCATT GCAGTTGCAA CAAGTCAGGG TCTGGTGGTG 
GTGTGGGAGC TGCAGCTGGA GCGGCGAGGT CGTCCGGAGC GCGTCAGCGT GTCGTCGGAG 
CACAGAGGAC ACAACATCAC CGCCCTCTGC TGGGATACAA GCACACTTAG GGTTTTTGTT 
GGGGACTCTG TAGGCAAGGT GTCGTGTCTG CGGGCGGGAT CATCTAAACT GGGCAAGGGG 
TCAGCATTTG TTATTTTTCC TGTCCAAACT GTTACTACTG TTGACTCCAG AGTAGTTCAG 
CTTGGATATC AAGACGGGCG CCTCCTAGTG TCTTCTCTCA GCCGCTGCTA CCTCTGTGAC 
ACAGAGAAGG AGAAATTCTG GCGTGTTGGG AATAAGGAGC GTGACGGGGA ATACGGAGCC 
TGCTTCTTGC CCCAGGGTAG GGGGTTATTA GTGGGACAGC CCCCGCTGCT GTACTGCGCC 
CGGCCGGGGT CCCGAATATG GGAGGCTAAT TTTAACGGCG AGGTTCTGAG TACTCATCAA 
TTCAAAAAGC TGCTCGCCTG CCCACCACTC CCTTTAATCA CTTACAGAAA CGATCCAAAT 
TACAATCCAG CACAGAGTAG TCCGCAGTCT CTCGGCTTCC CTAAACTGCT TTATTTTGGA 
GATCAAAATC TGCTTACTTG GACCGATTCG GCCGTTTATA TCTTCACACC TCAGAATGGA 
CAGGTCCTGC TGTGGACTGA AGTTAAAGAC GTGGCTGACA TTGCAGTCCA CCGCAGCGAG 
CTGTTCTGCC TCCACGGCAG TGGCCGTCTG TCCCATCTGT CTTTGCTGTC TGCGGAGCGC 
TGCGTGGAAC GCCTGCTGCG TAGAGAGTCC TGGACTCTCG CTGCCACCGT CTGCTGCATG 
TTCCAGCACA CGATCACGAC GAGCCGGGCC AGGAAGTCCA TTCCCATCGA TCGCCTGGAA 
CACCTGAAGT CTCAGCTCAG TTCCAGCGCA CAGACTGAGC TGATTAACCA GCTGGAGGAA 
GTGATCCTGA AGCTGGAGCC ACTGGATTCT GCATCTAGCA GCCGCAGGAG CAGCATCTCT 
TCACATGAGA GTTTTAACGT CCTGGACTGT GGGATCTACC GGGTTATCAG CCGCAGAGGA 
AGCCAGTCGG ACGAGGAGAC CAGCTCCCTC ATGAATCAGT CTGCGTCAGA GGAGGAGCGA 
CTGAAAGAGT TCAGCTTTGT CCAGGAGGAA GACCAGGTGG ATCATGATCC ACAGAGCGGA 
GAGCGGCTGG AGGCCGATCG ACCTGACCAA GGCCTGCAGT TCCACCTCCC ACTTTCATTC 
CGCACCAAAC CCCCCCGCAT AGCTTTGCAG GCCGTTAAAG ACAGCGTTTC TAGTTTTGTC 
AAGAAGACGA CTGAGAAGAT CAACACCCTC CAGATGAACT CTGAACTCCG ACATCGGCCT 
GAATTTAAAG AGAGCGCACA GCCCGACACG TTAGCCGGCA CGTTATCGTA CTCAGACGAA 
ATGGAGAACG AAGTTTATAA TGAAATATCA AACGCAGAGG CTGATATGGA TGAACTTCGT 
TCAGCAACAG AACAAGCTGT CTCACAGATA CAAGATCCTT TGGTGCTCCT GGATCCAGTC 
TGCCTTAGAG AAGCTCTGAT GGAGTGGCTC CCTGTGTTGG AGCGAATCCT GGGCCCTGCT 
GTTCTCCTGA CAGCTTCTGT AACCAATTCC AACCTGGATG GATCTGGAGA AGAGAAATGG 
GACAGGGAAT ATCTGAGCCC ATGCACAGAG CAACAAAGAG AATCATCAGA AAGTGTTGCA 
GAGGAGGCGG AGCTCGGAGA AAAAGAAGTC CTCTGTGAGA AACAGCCGAT GGTTGACACG 
ACTGAAAATG CCGCTTCTCT GGAGAATCAT CCGGAGCCCA TCCGAGTGGC GTCTCCTACG 
CCTTTGCCTT CAGATCTGAA AGCTAATCTC AGCGAGCTGG CTTCACTTTA CACAGAGCTC 
AGCTGCTTCA GGCACCAGCA GGAGGTGCTG GAAAGCGCAA CTTTCCTGCG TCGTTACTTT 
TTCCTTCTGG ACCAGGAGCG AGTGAGAAGA ATGTGCCTTC TTTGTTATCA GCAGCGACCC 
GAGATGATGA GCTCGTTCTC TGAGGCCATG CTAGCTCTCA CTCAGTCCAG CAAGGTGGTT 
GAAATTATTC AGAGAGGAGA TTTGCTGAAA TCTCTGCGAA GCCTCAGAGA GCTGCAGCCT 
TGGGATGCGG CGCATCTCCT CGCTCACTTC CACAGGCTAT ACGAGAAGCA TGGTGAGGCA 
GCTGTACGCT CGTTTGCCCA GTTCTATCCC ATTATGACTC CTGCTGACGT GGAGACGGTG 
GCTCAGCGAA GCCACTTCCT GGCGTACCTG GATAATCTTG TACATTCAAG AGCTGAGGAG 
CACAAGTTGT CATTTCTTCA GTCGCTCCTT GAACCTGAAT CACTGAGACA GGATTGGCTG 
GAGCTGGCCC TGACTCACGA TGCCCCTCAA CACGCCGATA CTCTGACCTC TGATGGAAGG 
CCCAGGTGGC GCTCACACCG TTTCACCTGG GGCTACGGAC GTCTTTTGTC TTTGCTCATT 
CGACTTCCTG CAGACATTTC ATCCAAAGAG AAGATGAATG AGATGTGCCA CAGTCACGGG 
TACTGGACGG GTTACCTCTA CCTCTGCAAA GAGCTGCAGC GTTGCTCTGA AGCATTTTCC 
ACCATCTGTC AGCTGGACGA CCTCAGCCTC CTGGAGGATC CTGATGGTGT TGAACCTCAG 
AACCTGGACG AGTGGAAGCT GCTGATCCAG CTCTCTCAGC AGTATTGCCG TGTGGCAGAC 
CCACCGCCGG CCACAGATGT GAACGGGAGC AGCTGGTCCA ACGGCGCCAC GGACTGCGAA 
GGACGGATCA ACCCGGAGAG TCTGACCCTC CTGCTGGCTC GGACGCTGGG GCCTGACCGA 
GCCCTGGAGC TGCTGCGCGA ATGTGGAGTG CAGGTGGTCC TTTCAGCCCA CTCCAAGCTG 
GTGTGTGACC TGCTGAGAGT CGCAGAGAGG AGGCAGAGGG CGATGATTCA GACCATGCTG 
GAGCGCTGCG ATCGGTTCCT CTGGTCTCAG CATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008410310.1
CDS	239..3577
Translation	MPVIPVVPENHSHLLAEFDCLDPLLSALRFDSGRLKCTCLAVSRKWLALGTSAGGLHLIQKEGWKQRLILTHKEGSITQVACCPHDEDFIAVATSQGLVVVWELQLERRGRPERVSVSSEHRGHNITALCWDTSTLRVFVGDSVGKVSCLRAGSSKLGKGSAFVIFPVQTVTTVDSRVVQLGYQDGRLLVSSLSRCYLCDTEKEKFWRVGNKERDGEYGACFLPQGRGLLVGQPPLLYCARPGSRIWEANFNGEVLSTHQFKKLLACPPLPLITYRNDPNYNPAQSSPQSLGFPKLLYFGDQNLLTWTDSAVYIFTPQNGQVLLWTEVKDVADIAVHRSELFCLHGSGRLSHLSLLSAERCVERLLRRESWTLAATVCCMFQHTITTSRARKSIPIDRLEHLKSQLSSSAQTELINQLEEVILKLEPLDSASSSRRSSISSHESFNVLDCGIYRVISRRGSQSDEETSSLMNQSASEEERLKEFSFVQEEDQVDHDPQSGERLEADRPDQGLQFHLPLSFRTKPPRIALQAVKDSVSSFVKKTTEKINTLQMNSELRHRPEFKESAQPDTLAGTLSYSDEMENEVYNEISNAEADMDELRSATEQAVSQIQDPLVLLDPVCLREALMEWLPVLERILGPAVLLTASVTNSNLDGSGEEKWDREYLSPCTEQQRESSESVAEEAELGEKEVLCEKQPMVDTTENAASLENHPEPIRVASPTPLPSDLKANLSELASLYTELSCFRHQQEVLESATFLRRYFFLLDQERVRRMCLLCYQQRPEMMSSFSEAMLALTQSSKVVEIIQRGDLLKSLRSLRELQPWDAAHLLAHFHRLYEKHGEAAVRSFAQFYPIMTPADVETVAQRSHFLAYLDNLVHSRAEEHKLSFLQSLLEPESLRQDWLELALTHDAPQHADTLTSDGRPRWRSHRFTWGYGRLLSLLIRLPADISSKEKMNEMCHSHGYWTGYLYLCKELQRCSEAFSTICQLDDLSLLEDPDGVEPQNLDEWKLLIQLSQQYCRVADPPPATDVNGSSWSNGATDCEGRINPESLTLLLARTLGPDRALELLRECGVQVVLSAHSKLVCDLLRVAERRQRAMIQTMLERCDRFLWSQHA

Target ORF information:

RefSeq Version	XM_008412088.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 (hps5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008412088.2

ORF Insert Sequence:

ATGCCTGTGA TACCGGTTGT GCCTGAGAAC CACAGTCACC TCCTGGCAGA GTTCGACTGC 
CTGGATCCGC TCCTCTCTGC TCTGCGTTTT GACTCAGGCA GGCTGAAGTG TACCTGTCTG 
GCGGTGTCCA GGAAGTGGCT CGCGTTGGGA ACGTCAGCAG GAGGGTTGCA CCTCATCCAG 
AAGGAGGGCT GGAAACAGAG ACTCATTCTG ACTCACAAGG AGGGATCTAT CACACAAGTG 
GCATGTTGTC CTCATGATGA AGATTTCATT GCAGTTGCAA CAAGTCAGGG TCTGGTGGTG 
GTGTGGGAGC TGCAGCTGGA GCGGCGAGGT CGTCCGGAGC GCGTCAGCGT GTCGTCGGAG 
CACAGAGGAC ACAACATCAC CGCCCTCTGC TGGGATACAA GCACACTTAG GGTTTTTGTT 
GGGGACTCTG TAGGCAAGGT GTCGTGTCTG CGGGCGGGAT CATCTAAACT GGGCAAGGGG 
TCAGCATTTG TTATTTTTCC TGTCCAAACT GTTACTACTG TTGACTCCAG AGTAGTTCAG 
CTTGGATATC AAGACGGGCG CCTCCTAGTG TCTTCTCTCA GCCGCTGCTA CCTCTGTGAC 
ACAGAGAAGG AGAAATTCTG GCGTGTTGGG AATAAGGAGC GTGACGGGGA ATACGGAGCC 
TGCTTCTTGC CCCAGGGTAG GGGGTTATTA GTGGGACAGC CCCCGCTGCT GTACTGCGCC 
CGGCCGGGGT CCCGAATATG GGAGGCTAAT TTTAACGGCG AGGTTCTGAG TACTCATCAA 
TTCAAAAAGC TGCTCGCCTG CCCACCACTC CCTTTAATCA CTTACAGAAA CGATCCAAAT 
TACAATCCAG CACAGAGTAG TCCGCAGTCT CTCGGCTTCC CTAAACTGCT TTATTTTGGA 
GATCAAAATC TGCTTACTTG GACCGATTCG GCCGTTTATA TCTTCACACC TCAGAATGGA 
CAGGTCCTGC TGTGGACTGA AGTTAAAGAC GTGGCTGACA TTGCAGTCCA CCGCAGCGAG 
CTGTTCTGCC TCCACGGCAG TGGCCGTCTG TCCCATCTGT CTTTGCTGTC TGCGGAGCGC 
TGCGTGGAAC GCCTGCTGCG TAGAGAGTCC TGGACTCTCG CTGCCACCGT CTGCTGCATG 
TTCCAGCACA CGATCACGAC GAGCCGGGCC AGGAAGTCCA TTCCCATCGA TCGCCTGGAA 
CACCTGAAGT CTCAGCTCAG TTCCAGCGCA CAGACTGAGC TGATTAACCA GCTGGAGGAA 
GTGATCCTGA AGCTGGAGCC ACTGGATTCT GCATCTAGCA GCCGCAGGAG CAGCATCTCT 
TCACATGAGA GTTTTAACGT CCTGGACTGT GGGATCTACC GGGTTATCAG CCGCAGAGGA 
AGCCAGTCGG ACGAGGAGAC CAGCTCCCTC ATGAATCAGT CTGCGTCAGA GGAGGAGCGA 
CTGAAAGAGT TCAGCTTTGT CCAGGAGGAA GACCAGGTGG ATCATGATCC ACAGAGCGGA 
GAGCGGCTGG AGGCCGATCG ACCTGACCAA GGCCTGCAGT TCCACCTCCC ACTTTCATTC 
CGCACCAAAC CCCCCCGCAT AGCTTTGCAG GCCGTTAAAG ACAGCGTTTC TAGTTTTGTC 
AAGAAGACGA CTGAGAAGAT CAACACCCTC CAGATGAACT CTGAACTCCG ACATCGGCCT 
GAATTTAAAG AGAGCGCACA GCCCGACACG TTAGCCGGCA CGTTATCGTA CTCAGACGAA 
ATGGAGAACG AAGTTTATAA TGAAATATCA AACGCAGAGG CTGATATGGA TGAACTTCGT 
TCAGCAACAG AACAAGCTGT CTCACAGATA CAAGATCCTT TGGTGCTCCT GGATCCAGTC 
TGCCTTAGAG AAGCTCTGAT GGAGTGGCTC CCTGTGTTGG AGCGAATCCT GGGCCCTGCT 
GTTCTCCTGA CAGCTTCTGT AACCAATTCC AACCTGGATG GATCTGGAGA AGAGAAATGG 
GACAGGGAAT ATCTGAGCCC ATGCACAGAG CAACAAAGAG AATCATCAGA AAGTGTTGCA 
GAGGAGGCGG AGCTCGGAGA AAAAGAAGTC CTCTGTGAGA AACAGCCGAT GGTTGACACG 
ACTGAAAATG CCGCTTCTCT GGAGAATCAT CCGGAGCCCA TCCGAGTGGC GTCTCCTACG 
CCTTTGCCTT CAGATCTGAA AGCTAATCTC AGCGAGCTGG CTTCACTTTA CACAGAGCTC 
AGCTGCTTCA GGCACCAGCA GGAGGTGCTG GAAAGCGCAA CTTTCCTGCG TCGTTACTTT 
TTCCTTCTGG ACCAGGAGCG AGTGAGAAGA ATGTGCCTTC TTTGTTATCA GCAGCGACCC 
GAGATGATGA GCTCGTTCTC TGAGGCCATG CTAGCTCTCA CTCAGTCCAG CAAGGTGGTT 
GAAATTATTC AGAGAGGAGA TTTGCTGAAA TCTCTGCGAA GCCTCAGAGA GCTGCAGCCT 
TGGGATGCGG CGCATCTCCT CGCTCACTTC CACAGGCTAT ACGAGAAGCA TGGTGAGGCA 
GCTGTACGCT CGTTTGCCCA GTTCTATCCC ATTATGACTC CTGCTGACGT GGAGACGGTG 
GCTCAGCGAA GCCACTTCCT GGCGTACCTG GATAATCTTG TACATTCAAG AGCTGAGGAG 
CACAAGTTGT CATTTCTTCA GTCGCTCCTT GAACCTGAAT CACTGAGACA GGATTGGCTG 
GAGCTGGCCC TGACTCACGA TGCCCCTCAA CACGCCGATA CTCTGACCTC TGATGGAAGG 
CCCAGGTGGC GCTCACACCG TTTCACCTGG GGCTACGGAC GTCTTTTGTC TTTGCTCATT 
CGACTTCCTG CAGACATTTC ATCCAAAGAG AAGATGAATG AGATGTGCCA CAGTCACGGG 
TACTGGACGG GTTACCTCTA CCTCTGCAAA GAGCTGCAGC GTTGCTCTGA AGCATTTTCC 
ACCATCTGTC AGCTGGACGA CCTCAGCCTC CTGGAGGATC CTGATGGTGT TGAACCTCAG 
AACCTGGACG AGTGGAAGCT GCTGATCCAG CTCTCTCAGC AGTATTGCCG TGTGGCAGAC 
CCACCGCCGG CCACAGATGT GAACGGGAGC AGCTGGTCCA ACGGCGCCAC GGACTGCGAA 
GGACGGATCA ACCCGGAGAG TCTGACCCTC CTGCTGGCTC GGACGCTGGG GCCTGACCGA 
GCCCTGGAGC TGCTGCGCGA ATGTGGAGTG CAGGTGGTCC TTTCAGCCCA CTCCAAGCTG 
GTGTGTGACC TGCTGAGAGT CGCAGAGAGG AGGCAGAGGG CGATGATTCA GACCATGCTG 
GAGCGCTGCG ATCGGTTCCT CTGGTCTCAG CATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hps5 ( XM_008412089.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008410311.1 Poecilia reticulata HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 (hps5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo197298
Clone ID Related Accession (Same CDS sequence)	XM_008412088.2 , XM_008412089.2
Accession Version	XM_008412089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	Hermansky-Pudlak syndrome 5 protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008412089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTGA TACCGGTTGT GCCTGAGAAC CACAGTCACC TCCTGGCAGA GTTCGACTGC 
CTGGATCCGC TCCTCTCTGC TCTGCGTTTT GACTCAGGCA GGCTGAAGTG TACCTGTCTG 
GCGGTGTCCA GGAAGTGGCT CGCGTTGGGA ACGTCAGCAG GAGGGTTGCA CCTCATCCAG 
AAGGAGGGCT GGAAACAGAG ACTCATTCTG ACTCACAAGG AGGGATCTAT CACACAAGTG 
GCATGTTGTC CTCATGATGA AGATTTCATT GCAGTTGCAA CAAGTCAGGG TCTGGTGGTG 
GTGTGGGAGC TGCAGCTGGA GCGGCGAGGT CGTCCGGAGC GCGTCAGCGT GTCGTCGGAG 
CACAGAGGAC ACAACATCAC CGCCCTCTGC TGGGATACAA GCACACTTAG GGTTTTTGTT 
GGGGACTCTG TAGGCAAGGT GTCGTGTCTG CGGGCGGGAT CATCTAAACT GGGCAAGGGG 
TCAGCATTTG TTATTTTTCC TGTCCAAACT GTTACTACTG TTGACTCCAG AGTAGTTCAG 
CTTGGATATC AAGACGGGCG CCTCCTAGTG TCTTCTCTCA GCCGCTGCTA CCTCTGTGAC 
ACAGAGAAGG AGAAATTCTG GCGTGTTGGG AATAAGGAGC GTGACGGGGA ATACGGAGCC 
TGCTTCTTGC CCCAGGGTAG GGGGTTATTA GTGGGACAGC CCCCGCTGCT GTACTGCGCC 
CGGCCGGGGT CCCGAATATG GGAGGCTAAT TTTAACGGCG AGGTTCTGAG TACTCATCAA 
TTCAAAAAGC TGCTCGCCTG CCCACCACTC CCTTTAATCA CTTACAGAAA CGATCCAAAT 
TACAATCCAG CACAGAGTAG TCCGCAGTCT CTCGGCTTCC CTAAACTGCT TTATTTTGGA 
GATCAAAATC TGCTTACTTG GACCGATTCG GCCGTTTATA TCTTCACACC TCAGAATGGA 
CAGGTCCTGC TGTGGACTGA AGTTAAAGAC GTGGCTGACA TTGCAGTCCA CCGCAGCGAG 
CTGTTCTGCC TCCACGGCAG TGGCCGTCTG TCCCATCTGT CTTTGCTGTC TGCGGAGCGC 
TGCGTGGAAC GCCTGCTGCG TAGAGAGTCC TGGACTCTCG CTGCCACCGT CTGCTGCATG 
TTCCAGCACA CGATCACGAC GAGCCGGGCC AGGAAGTCCA TTCCCATCGA TCGCCTGGAA 
CACCTGAAGT CTCAGCTCAG TTCCAGCGCA CAGACTGAGC TGATTAACCA GCTGGAGGAA 
GTGATCCTGA AGCTGGAGCC ACTGGATTCT GCATCTAGCA GCCGCAGGAG CAGCATCTCT 
TCACATGAGA GTTTTAACGT CCTGGACTGT GGGATCTACC GGGTTATCAG CCGCAGAGGA 
AGCCAGTCGG ACGAGGAGAC CAGCTCCCTC ATGAATCAGT CTGCGTCAGA GGAGGAGCGA 
CTGAAAGAGT TCAGCTTTGT CCAGGAGGAA GACCAGGTGG ATCATGATCC ACAGAGCGGA 
GAGCGGCTGG AGGCCGATCG ACCTGACCAA GGCCTGCAGT TCCACCTCCC ACTTTCATTC 
CGCACCAAAC CCCCCCGCAT AGCTTTGCAG GCCGTTAAAG ACAGCGTTTC TAGTTTTGTC 
AAGAAGACGA CTGAGAAGAT CAACACCCTC CAGATGAACT CTGAACTCCG ACATCGGCCT 
GAATTTAAAG AGAGCGCACA GCCCGACACG TTAGCCGGCA CGTTATCGTA CTCAGACGAA 
ATGGAGAACG AAGTTTATAA TGAAATATCA AACGCAGAGG CTGATATGGA TGAACTTCGT 
TCAGCAACAG AACAAGCTGT CTCACAGATA CAAGATCCTT TGGTGCTCCT GGATCCAGTC 
TGCCTTAGAG AAGCTCTGAT GGAGTGGCTC CCTGTGTTGG AGCGAATCCT GGGCCCTGCT 
GTTCTCCTGA CAGCTTCTGT AACCAATTCC AACCTGGATG GATCTGGAGA AGAGAAATGG 
GACAGGGAAT ATCTGAGCCC ATGCACAGAG CAACAAAGAG AATCATCAGA AAGTGTTGCA 
GAGGAGGCGG AGCTCGGAGA AAAAGAAGTC CTCTGTGAGA AACAGCCGAT GGTTGACACG 
ACTGAAAATG CCGCTTCTCT GGAGAATCAT CCGGAGCCCA TCCGAGTGGC GTCTCCTACG 
CCTTTGCCTT CAGATCTGAA AGCTAATCTC AGCGAGCTGG CTTCACTTTA CACAGAGCTC 
AGCTGCTTCA GGCACCAGCA GGAGGTGCTG GAAAGCGCAA CTTTCCTGCG TCGTTACTTT 
TTCCTTCTGG ACCAGGAGCG AGTGAGAAGA ATGTGCCTTC TTTGTTATCA GCAGCGACCC 
GAGATGATGA GCTCGTTCTC TGAGGCCATG CTAGCTCTCA CTCAGTCCAG CAAGGTGGTT 
GAAATTATTC AGAGAGGAGA TTTGCTGAAA TCTCTGCGAA GCCTCAGAGA GCTGCAGCCT 
TGGGATGCGG CGCATCTCCT CGCTCACTTC CACAGGCTAT ACGAGAAGCA TGGTGAGGCA 
GCTGTACGCT CGTTTGCCCA GTTCTATCCC ATTATGACTC CTGCTGACGT GGAGACGGTG 
GCTCAGCGAA GCCACTTCCT GGCGTACCTG GATAATCTTG TACATTCAAG AGCTGAGGAG 
CACAAGTTGT CATTTCTTCA GTCGCTCCTT GAACCTGAAT CACTGAGACA GGATTGGCTG 
GAGCTGGCCC TGACTCACGA TGCCCCTCAA CACGCCGATA CTCTGACCTC TGATGGAAGG 
CCCAGGTGGC GCTCACACCG TTTCACCTGG GGCTACGGAC GTCTTTTGTC TTTGCTCATT 
CGACTTCCTG CAGACATTTC ATCCAAAGAG AAGATGAATG AGATGTGCCA CAGTCACGGG 
TACTGGACGG GTTACCTCTA CCTCTGCAAA GAGCTGCAGC GTTGCTCTGA AGCATTTTCC 
ACCATCTGTC AGCTGGACGA CCTCAGCCTC CTGGAGGATC CTGATGGTGT TGAACCTCAG 
AACCTGGACG AGTGGAAGCT GCTGATCCAG CTCTCTCAGC AGTATTGCCG TGTGGCAGAC 
CCACCGCCGG CCACAGATGT GAACGGGAGC AGCTGGTCCA ACGGCGCCAC GGACTGCGAA 
GGACGGATCA ACCCGGAGAG TCTGACCCTC CTGCTGGCTC GGACGCTGGG GCCTGACCGA 
GCCCTGGAGC TGCTGCGCGA ATGTGGAGTG CAGGTGGTCC TTTCAGCCCA CTCCAAGCTG 
GTGTGTGACC TGCTGAGAGT CGCAGAGAGG AGGCAGAGGG CGATGATTCA GACCATGCTG 
GAGCGCTGCG ATCGGTTCCT CTGGTCTCAG CATGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008410311.1
CDS	209..3547
Translation	MPVIPVVPENHSHLLAEFDCLDPLLSALRFDSGRLKCTCLAVSRKWLALGTSAGGLHLIQKEGWKQRLILTHKEGSITQVACCPHDEDFIAVATSQGLVVVWELQLERRGRPERVSVSSEHRGHNITALCWDTSTLRVFVGDSVGKVSCLRAGSSKLGKGSAFVIFPVQTVTTVDSRVVQLGYQDGRLLVSSLSRCYLCDTEKEKFWRVGNKERDGEYGACFLPQGRGLLVGQPPLLYCARPGSRIWEANFNGEVLSTHQFKKLLACPPLPLITYRNDPNYNPAQSSPQSLGFPKLLYFGDQNLLTWTDSAVYIFTPQNGQVLLWTEVKDVADIAVHRSELFCLHGSGRLSHLSLLSAERCVERLLRRESWTLAATVCCMFQHTITTSRARKSIPIDRLEHLKSQLSSSAQTELINQLEEVILKLEPLDSASSSRRSSISSHESFNVLDCGIYRVISRRGSQSDEETSSLMNQSASEEERLKEFSFVQEEDQVDHDPQSGERLEADRPDQGLQFHLPLSFRTKPPRIALQAVKDSVSSFVKKTTEKINTLQMNSELRHRPEFKESAQPDTLAGTLSYSDEMENEVYNEISNAEADMDELRSATEQAVSQIQDPLVLLDPVCLREALMEWLPVLERILGPAVLLTASVTNSNLDGSGEEKWDREYLSPCTEQQRESSESVAEEAELGEKEVLCEKQPMVDTTENAASLENHPEPIRVASPTPLPSDLKANLSELASLYTELSCFRHQQEVLESATFLRRYFFLLDQERVRRMCLLCYQQRPEMMSSFSEAMLALTQSSKVVEIIQRGDLLKSLRSLRELQPWDAAHLLAHFHRLYEKHGEAAVRSFAQFYPIMTPADVETVAQRSHFLAYLDNLVHSRAEEHKLSFLQSLLEPESLRQDWLELALTHDAPQHADTLTSDGRPRWRSHRFTWGYGRLLSLLIRLPADISSKEKMNEMCHSHGYWTGYLYLCKELQRCSEAFSTICQLDDLSLLEDPDGVEPQNLDEWKLLIQLSQQYCRVADPPPATDVNGSSWSNGATDCEGRINPESLTLLLARTLGPDRALELLRECGVQVVLSAHSKLVCDLLRVAERRQRAMIQTMLERCDRFLWSQHA

Target ORF information:

RefSeq Version	XM_008412089.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata HPS5, biogenesis of lysosomal organelles complex 2 subunit 2 (hps5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008412089.2

ORF Insert Sequence:

ATGCCTGTGA TACCGGTTGT GCCTGAGAAC CACAGTCACC TCCTGGCAGA GTTCGACTGC 
CTGGATCCGC TCCTCTCTGC TCTGCGTTTT GACTCAGGCA GGCTGAAGTG TACCTGTCTG 
GCGGTGTCCA GGAAGTGGCT CGCGTTGGGA ACGTCAGCAG GAGGGTTGCA CCTCATCCAG 
AAGGAGGGCT GGAAACAGAG ACTCATTCTG ACTCACAAGG AGGGATCTAT CACACAAGTG 
GCATGTTGTC CTCATGATGA AGATTTCATT GCAGTTGCAA CAAGTCAGGG TCTGGTGGTG 
GTGTGGGAGC TGCAGCTGGA GCGGCGAGGT CGTCCGGAGC GCGTCAGCGT GTCGTCGGAG 
CACAGAGGAC ACAACATCAC CGCCCTCTGC TGGGATACAA GCACACTTAG GGTTTTTGTT 
GGGGACTCTG TAGGCAAGGT GTCGTGTCTG CGGGCGGGAT CATCTAAACT GGGCAAGGGG 
TCAGCATTTG TTATTTTTCC TGTCCAAACT GTTACTACTG TTGACTCCAG AGTAGTTCAG 
CTTGGATATC AAGACGGGCG CCTCCTAGTG TCTTCTCTCA GCCGCTGCTA CCTCTGTGAC 
ACAGAGAAGG AGAAATTCTG GCGTGTTGGG AATAAGGAGC GTGACGGGGA ATACGGAGCC 
TGCTTCTTGC CCCAGGGTAG GGGGTTATTA GTGGGACAGC CCCCGCTGCT GTACTGCGCC 
CGGCCGGGGT CCCGAATATG GGAGGCTAAT TTTAACGGCG AGGTTCTGAG TACTCATCAA 
TTCAAAAAGC TGCTCGCCTG CCCACCACTC CCTTTAATCA CTTACAGAAA CGATCCAAAT 
TACAATCCAG CACAGAGTAG TCCGCAGTCT CTCGGCTTCC CTAAACTGCT TTATTTTGGA 
GATCAAAATC TGCTTACTTG GACCGATTCG GCCGTTTATA TCTTCACACC TCAGAATGGA 
CAGGTCCTGC TGTGGACTGA AGTTAAAGAC GTGGCTGACA TTGCAGTCCA CCGCAGCGAG 
CTGTTCTGCC TCCACGGCAG TGGCCGTCTG TCCCATCTGT CTTTGCTGTC TGCGGAGCGC 
TGCGTGGAAC GCCTGCTGCG TAGAGAGTCC TGGACTCTCG CTGCCACCGT CTGCTGCATG 
TTCCAGCACA CGATCACGAC GAGCCGGGCC AGGAAGTCCA TTCCCATCGA TCGCCTGGAA 
CACCTGAAGT CTCAGCTCAG TTCCAGCGCA CAGACTGAGC TGATTAACCA GCTGGAGGAA 
GTGATCCTGA AGCTGGAGCC ACTGGATTCT GCATCTAGCA GCCGCAGGAG CAGCATCTCT 
TCACATGAGA GTTTTAACGT CCTGGACTGT GGGATCTACC GGGTTATCAG CCGCAGAGGA 
AGCCAGTCGG ACGAGGAGAC CAGCTCCCTC ATGAATCAGT CTGCGTCAGA GGAGGAGCGA 
CTGAAAGAGT TCAGCTTTGT CCAGGAGGAA GACCAGGTGG ATCATGATCC ACAGAGCGGA 
GAGCGGCTGG AGGCCGATCG ACCTGACCAA GGCCTGCAGT TCCACCTCCC ACTTTCATTC 
CGCACCAAAC CCCCCCGCAT AGCTTTGCAG GCCGTTAAAG ACAGCGTTTC TAGTTTTGTC 
AAGAAGACGA CTGAGAAGAT CAACACCCTC CAGATGAACT CTGAACTCCG ACATCGGCCT 
GAATTTAAAG AGAGCGCACA GCCCGACACG TTAGCCGGCA CGTTATCGTA CTCAGACGAA 
ATGGAGAACG AAGTTTATAA TGAAATATCA AACGCAGAGG CTGATATGGA TGAACTTCGT 
TCAGCAACAG AACAAGCTGT CTCACAGATA CAAGATCCTT TGGTGCTCCT GGATCCAGTC 
TGCCTTAGAG AAGCTCTGAT GGAGTGGCTC CCTGTGTTGG AGCGAATCCT GGGCCCTGCT 
GTTCTCCTGA CAGCTTCTGT AACCAATTCC AACCTGGATG GATCTGGAGA AGAGAAATGG 
GACAGGGAAT ATCTGAGCCC ATGCACAGAG CAACAAAGAG AATCATCAGA AAGTGTTGCA 
GAGGAGGCGG AGCTCGGAGA AAAAGAAGTC CTCTGTGAGA AACAGCCGAT GGTTGACACG 
ACTGAAAATG CCGCTTCTCT GGAGAATCAT CCGGAGCCCA TCCGAGTGGC GTCTCCTACG 
CCTTTGCCTT CAGATCTGAA AGCTAATCTC AGCGAGCTGG CTTCACTTTA CACAGAGCTC 
AGCTGCTTCA GGCACCAGCA GGAGGTGCTG GAAAGCGCAA CTTTCCTGCG TCGTTACTTT 
TTCCTTCTGG ACCAGGAGCG AGTGAGAAGA ATGTGCCTTC TTTGTTATCA GCAGCGACCC 
GAGATGATGA GCTCGTTCTC TGAGGCCATG CTAGCTCTCA CTCAGTCCAG CAAGGTGGTT 
GAAATTATTC AGAGAGGAGA TTTGCTGAAA TCTCTGCGAA GCCTCAGAGA GCTGCAGCCT 
TGGGATGCGG CGCATCTCCT CGCTCACTTC CACAGGCTAT ACGAGAAGCA TGGTGAGGCA 
GCTGTACGCT CGTTTGCCCA GTTCTATCCC ATTATGACTC CTGCTGACGT GGAGACGGTG 
GCTCAGCGAA GCCACTTCCT GGCGTACCTG GATAATCTTG TACATTCAAG AGCTGAGGAG 
CACAAGTTGT CATTTCTTCA GTCGCTCCTT GAACCTGAAT CACTGAGACA GGATTGGCTG 
GAGCTGGCCC TGACTCACGA TGCCCCTCAA CACGCCGATA CTCTGACCTC TGATGGAAGG 
CCCAGGTGGC GCTCACACCG TTTCACCTGG GGCTACGGAC GTCTTTTGTC TTTGCTCATT 
CGACTTCCTG CAGACATTTC ATCCAAAGAG AAGATGAATG AGATGTGCCA CAGTCACGGG 
TACTGGACGG GTTACCTCTA CCTCTGCAAA GAGCTGCAGC GTTGCTCTGA AGCATTTTCC 
ACCATCTGTC AGCTGGACGA CCTCAGCCTC CTGGAGGATC CTGATGGTGT TGAACCTCAG 
AACCTGGACG AGTGGAAGCT GCTGATCCAG CTCTCTCAGC AGTATTGCCG TGTGGCAGAC 
CCACCGCCGG CCACAGATGT GAACGGGAGC AGCTGGTCCA ACGGCGCCAC GGACTGCGAA 
GGACGGATCA ACCCGGAGAG TCTGACCCTC CTGCTGGCTC GGACGCTGGG GCCTGACCGA 
GCCCTGGAGC TGCTGCGCGA ATGTGGAGTG CAGGTGGTCC TTTCAGCCCA CTCCAAGCTG 
GTGTGTGACC TGCTGAGAGT CGCAGAGAGG AGGCAGAGGG CGATGATTCA GACCATGCTG 
GAGCGCTGCG ATCGGTTCCT CTGGTCTCAG CATGCCTAA