BTD cDNA ORF clone, Nestor notabilis(Kea)

Gene
Clones
gRNAs

Summary

Gene Symbol	BTD
Entrez Gene ID	104411675
Full Name	biotinidase
Gene Type	protein-coding
Organism	Nestor notabilis(Kea)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010022161.1	XP_010020463.1	biotinidase

Pathways from BioSystems
Gene Ontology

The following BTD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BTD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ONe117921	XM_010022161.1 Latest version!	Nestor notabilis biotinidase (BTD), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BTD ( XM_010022161.1 ) cDNA ORF clone, Nestor notabilis(Kea) -> XP_010020463.1 Nestor notabilis biotinidase (BTD), mRNA.

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CloneID	ONe117921
Clone ID Related Accession (Same CDS sequence)	XM_010022161.1
Accession Version	XM_010022161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Nestor notabilis(Kea)
Product	biotinidase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009943915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Nestor notabilis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATC TTTGCTGGAA GCTGTCCTTC TGCTTTTTTT GCTATCAAGT TGTCTCAGGA 
AGAGTTAAGA GGGAGGGGCA TTATATTGCT GCTGTGTACG AACATGAGTC CATACTGAGT 
CCTAACCCAA AAGCCCTGGT TGATCGACAG TCTGCGCTGG AGCTCATGGG CAGGAACCTA 
GACATCTATG AACAGCAAGC AGTGGCTGCT GCAAGACAGG GGGCACAGAT CATTGTTTTT 
CCTGAAGATG GAATCCATGG CTTCAACTTC ACCAGAAGCT CCATTTATCC TTACTTGGAT 
TTCGCTCTGC ATTCACAGTC TGTGAAGTGG AATCCGTGCA GAGAGCCGTA TTTGTTCAAT 
GACACAGAGG TTCTTCAGCG TCTGAGCTGC ATGGCACTGA AGAACAAAAT ATTCCTTGTG 
GCAAACTTGG GAACTAAGCA GCCCTGTGAG CACATAGATC CTCACTGTCC CTCTGATGGA 
AGATACCAGT TCAATACCAA CGTGGCGTTC AATGCTGATG GCATGCTGGT AGCCACGTAC 
CGCAAACACA ATCTGTACTT TGAATATGCT TTTGATACCC CACCAGAGCC TGACTATAAA 
GTCTTTGATA CCCCTTTTGC TGGCAAGTTT GGTATGTTCA CTTGCTTTGA TATACTTTTT 
TTTGAGCCTG CAGTGAACCT CATTAGAAAA TACAATTTGA AACAAGTTGT ATATCCAACT 
GCCTGGATGA ACCAGCTCCC GCTCCTTTCT GCTGTAGAGT TTCAACAAGC TTTTGCAACA 
GCTTTCAACA TCAATATTTT AGCAGCTAAT ATCCACCACC CTACCTTGGG CATGACAGGA 
AGTGGCATAT ACACGCCAGT CAAATCGTTC GTCTACCACA ACATGGAAAG TTACGGTGGC 
AAGCTCATAG TAGCAGAAAT TCCTGTGATT ACTACAGATC ATGAAACCAA TTTGGAGAGT 
AATGAAAAAG TCAGAGAGAA TTTGCATCAC TCATGCAAGA CTTCTATAGG CACCTCACTG 
GATGATCAAG TTTGCTTTAG AGAGGGAGAA GAGACCCCCT GCAGCGTGTC AGAAAAAGGA 
AACGAACAGT TACTTCCCGT ATTTTATTCG GAAATGATGT ATGACAACTT CACTTTTGTT 
CCTGTATGGG GGGAAAAAGG AGAGCTCCAA GTTTGTGCCA ATACCCTTTG TTGTTACTTA 
AATTATCAGA GAGCTGGTTT AACTGATGAA TTATATGCCC TGGGAGTTTT TGATGGGCTC 
CATACAGTGC ATGGCACGTA CTATGTCCAG GCCTGTGCGT TAGTGAAGTG TGGTGGTCTC 
AACTTCAGCA CTTGTGGAGA GGAGGTTACA CATGCCACTG CTCTGATAGA TTTCCAGCTA 
TGGGGAAATA TGAGTACCCG TTATATCTTT CCTTTACTGC TGACATCTGG TATTACCTTG 
GACTTTGCTG ATCACATGGG CTGGAAAAAC AACCACTATT TCATAAGCAA AAATAGAACA 
TCTTCTGGCC TACTGACAGC TGCTCTATAT GGACGATGGT ATGAAAAAGA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010020463.1
CDS	1..1554
Translation	MVDLCWKLSFCFFCYQVVSGRVKREGHYIAAVYEHESILSPNPKALVDRQSALELMGRNLDIYEQQAVAAARQGAQIIVFPEDGIHGFNFTRSSIYPYLDFALHSQSVKWNPCREPYLFNDTEVLQRLSCMALKNKIFLVANLGTKQPCEHIDPHCPSDGRYQFNTNVAFNADGMLVATYRKHNLYFEYAFDTPPEPDYKVFDTPFAGKFGMFTCFDILFFEPAVNLIRKYNLKQVVYPTAWMNQLPLLSAVEFQQAFATAFNINILAANIHHPTLGMTGSGIYTPVKSFVYHNMESYGGKLIVAEIPVITTDHETNLESNEKVRENLHHSCKTSIGTSLDDQVCFREGEETPCSVSEKGNEQLLPVFYSEMMYDNFTFVPVWGEKGELQVCANTLCCYLNYQRAGLTDELYALGVFDGLHTVHGTYYVQACALVKCGGLNFSTCGEEVTHATALIDFQLWGNMSTRYIFPLLLTSGITLDFADHMGWKNNHYFISKNRTSSGLLTAALYGRWYEKD

Target ORF information:

RefSeq Version	XM_010022161.1
Organism	Nestor notabilis(Kea)
Definition	Nestor notabilis biotinidase (BTD), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010022161.1

ORF Insert Sequence:

ATGGTGGATC TTTGCTGGAA GCTGTCCTTC TGCTTTTTTT GCTATCAAGT TGTCTCAGGA 
AGAGTTAAGA GGGAGGGGCA TTATATTGCT GCTGTGTACG AACATGAGTC CATACTGAGT 
CCTAACCCAA AAGCCCTGGT TGATCGACAG TCTGCGCTGG AGCTCATGGG CAGGAACCTA 
GACATCTATG AACAGCAAGC AGTGGCTGCT GCAAGACAGG GGGCACAGAT CATTGTTTTT 
CCTGAAGATG GAATCCATGG CTTCAACTTC ACCAGAAGCT CCATTTATCC TTACTTGGAT 
TTCGCTCTGC ATTCACAGTC TGTGAAGTGG AATCCGTGCA GAGAGCCGTA TTTGTTCAAT 
GACACAGAGG TTCTTCAGCG TCTGAGCTGC ATGGCACTGA AGAACAAAAT ATTCCTTGTG 
GCAAACTTGG GAACTAAGCA GCCCTGTGAG CACATAGATC CTCACTGTCC CTCTGATGGA 
AGATACCAGT TCAATACCAA CGTGGCGTTC AATGCTGATG GCATGCTGGT AGCCACGTAC 
CGCAAACACA ATCTGTACTT TGAATATGCT TTTGATACCC CACCAGAGCC TGACTATAAA 
GTCTTTGATA CCCCTTTTGC TGGCAAGTTT GGTATGTTCA CTTGCTTTGA TATACTTTTT 
TTTGAGCCTG CAGTGAACCT CATTAGAAAA TACAATTTGA AACAAGTTGT ATATCCAACT 
GCCTGGATGA ACCAGCTCCC GCTCCTTTCT GCTGTAGAGT TTCAACAAGC TTTTGCAACA 
GCTTTCAACA TCAATATTTT AGCAGCTAAT ATCCACCACC CTACCTTGGG CATGACAGGA 
AGTGGCATAT ACACGCCAGT CAAATCGTTC GTCTACCACA ACATGGAAAG TTACGGTGGC 
AAGCTCATAG TAGCAGAAAT TCCTGTGATT ACTACAGATC ATGAAACCAA TTTGGAGAGT 
AATGAAAAAG TCAGAGAGAA TTTGCATCAC TCATGCAAGA CTTCTATAGG CACCTCACTG 
GATGATCAAG TTTGCTTTAG AGAGGGAGAA GAGACCCCCT GCAGCGTGTC AGAAAAAGGA 
AACGAACAGT TACTTCCCGT ATTTTATTCG GAAATGATGT ATGACAACTT CACTTTTGTT 
CCTGTATGGG GGGAAAAAGG AGAGCTCCAA GTTTGTGCCA ATACCCTTTG TTGTTACTTA 
AATTATCAGA GAGCTGGTTT AACTGATGAA TTATATGCCC TGGGAGTTTT TGATGGGCTC 
CATACAGTGC ATGGCACGTA CTATGTCCAG GCCTGTGCGT TAGTGAAGTG TGGTGGTCTC 
AACTTCAGCA CTTGTGGAGA GGAGGTTACA CATGCCACTG CTCTGATAGA TTTCCAGCTA 
TGGGGAAATA TGAGTACCCG TTATATCTTT CCTTTACTGC TGACATCTGG TATTACCTTG 
GACTTTGCTG ATCACATGGG CTGGAAAAAC AACCACTATT TCATAAGCAA AAATAGAACA 
TCTTCTGGCC TACTGACAGC TGCTCTATAT GGACGATGGT ATGAAAAAGA CTAG