RNF220 cDNA ORF clone, Manis javanica(Malayan pangolin)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF220
Entrez Gene ID	108402664
Full Name	ring finger protein 220
Gene Type	protein-coding
Organism	Manis javanica(Malayan pangolin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017669346.1	XP_017524835.1	E3 ubiquitin-protein ligase RNF220

Pathways from BioSystems
Gene Ontology

The following RNF220 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF220 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMa167332	XM_017669346.1 Latest version!	Manis javanica ring finger protein 220 (RNF220), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF220 ( XM_017669346.1 ) cDNA ORF clone, Manis javanica(Malayan pangolin) -> XP_017524835.1 Manis javanica ring finger protein 220 (RNF220), mRNA.

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CloneID	OMa167332
Clone ID Related Accession (Same CDS sequence)	XM_017669346.1
Accession Version	XM_017669346.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1698bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-28
Organism	Manis javanica(Malayan pangolin)
Product	E3 ubiquitin-protein ligase RNF220
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016537559.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Manis javanica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCGT CCTACCTCCC CAATCCTCTG 
GCATCCCCGG CACTGATGGT CTTGGCGTCC ACGGCTGAGG CCAGCCGTGA TGCTTCCATC 
CCCTGTCAGC AGCCACGACC CTTTGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCACATT 
CCCTTCACCA ACGGCTCCTA CACCTTTGCC TCTATGTACC ATCGGCAGGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCTTTACTAC ACCTCCACCC TCAGTTTGCT 
CCCCCAAATC TAGATTGCAC CCCAATCAGT ATGCTGAACC ATAGTGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTACCAGT CAGCCTTTAC GCCAGCCAAG 
CGACTTAAGA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC CCAGCTGCCA TCTAGCTCCC CTGGTTCACT TAAGGTTGAT 
GATGCTGGGA AGAAGATCTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AACCTCATCT 
AGCCCAGAGG ATCGGAATGA TAGATGTAAG AAAAAGGCAG CAGCATTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG CCAGGTCCTG CTGAGGCCCA GCGAGTTGCA GGAGCACATG 
GAGCAAGAGC TGGAACAGCT GGCCCAGCTG CCCACCAGCA AGAATTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGTACCCC CAAGTCCCTC CTGTTGTCTG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCGA CAGCATCACC ACACTCCTCC GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGTCTG CCTGTTCTGT GCTTTGTTTT TGGTTTCTCC ATGCAGCTCG GATTGGGAAA 
ATGAAACGGA GGAAGCAAGA TGAAGGGCAG AGGGAAGGCT CCTGCATGGC TGAGGATGAT 
GCCGTGGACA TCGAGCATGA GAACAGCAAC CGCTTTGAGG AGTATGAGTG GTGCGGGCAG 
AAGCGGATAC GGGCCACCAC TCTCCTGGAA GGTGGTTTCC GAGGCTCTGG CTTCGTCATG 
TGCAGCGGCA AAGAGAATCC GGACAGTGAT GCTGATTTGG ATGTGGATGG GGATGACACT 
CTGGAGTATG GGAAGCCACA ATACACGGAG GCTGACATCA TCCCGTGCAC AGGCGAGGAG 
CCTGGTGAAG CCAAGGAGAG AGAGGCCCTC CGGGGCGCAG TCCTAAATGG CGGCCCCCCC 
AGCACACGCA TCACACCTGA GTTTTCTAAA TGGGCCAGTG ATGAGATGCC ATCTACCAGC 
AATGGTGAGA GCAGCAAGCA GGAGGCCATG CAGAAGACCT GCAAGAATAG CGACATCGAA 
AAAATCACCG AAGATTCAGC TGTGACCACA TTTGAGGCCC TGAAGGCTCG GGTCAGGGAA 
CTTGAACGGC AGCTATCCCG TGGGGACCGT TACAAATGCC TCATCTGCAT GGACTCATAC 
TCGATGCCCC TAACGTCCAT CCAGTGTTGG CATGTGCACT GTGAGGAGTG CTGGCTGCGG 
ACCCTGGGTG CCAAGAAGCT CTGTCCTCAG TGCAACACCA TCACAGCGCC TGGAGACCTT 
CGGAGAATCT ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017524835.1
CDS	117..1814
Translation	MDLHRAAFKMENSSYLPNPLASPALMVLASTAEASRDASIPCQQPRPFGVPVSVDKDVHIPFTNGSYTFASMYHRQGGVPGTFANRDFPPSLLHLHPQFAPPNLDCTPISMLNHSGVGAFRPFASTEDRESYQSAFTPAKRLKNCHDTESPHLRFSDADGKEYDFGTQLPSSSPGSLKVDDAGKKIFAVSGLISDRETSSSPEDRNDRCKKKAAALFDSQAPICPICQVLLRPSELQEHMEQELEQLAQLPTSKNSLLKDAMAPGTPKSLLLSASIKREGESPTASPHSSATDDLHHSDRYQSACSVLCFWFLHAARIGKMKRRKQDEGQREGSCMAEDDAVDIEHENSNRFEEYEWCGQKRIRATTLLEGGFRGSGFVMCSGKENPDSDADLDVDGDDTLEYGKPQYTEADIIPCTGEEPGEAKEREALRGAVLNGGPPSTRITPEFSKWASDEMPSTSNGESSKQEAMQKTCKNSDIEKITEDSAVTTFEALKARVRELERQLSRGDRYKCLICMDSYSMPLTSIQCWHVHCEECWLRTLGAKKLCPQCNTITAPGDLRRIYL

Target ORF information:

RefSeq Version	XM_017669346.1
Organism	Manis javanica(Malayan pangolin)
Definition	Manis javanica ring finger protein 220 (RNF220), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017669346.1

ORF Insert Sequence:

ATGGACTTAC ACCGGGCAGC CTTCAAGATG GAGAACTCGT CCTACCTCCC CAATCCTCTG 
GCATCCCCGG CACTGATGGT CTTGGCGTCC ACGGCTGAGG CCAGCCGTGA TGCTTCCATC 
CCCTGTCAGC AGCCACGACC CTTTGGTGTA CCTGTCTCAG TAGACAAGGA CGTGCACATT 
CCCTTCACCA ACGGCTCCTA CACCTTTGCC TCTATGTACC ATCGGCAGGG TGGGGTGCCA 
GGCACTTTTG CCAATCGTGA TTTTCCCCCT TCTTTACTAC ACCTCCACCC TCAGTTTGCT 
CCCCCAAATC TAGATTGCAC CCCAATCAGT ATGCTGAACC ATAGTGGAGT GGGGGCTTTC 
CGTCCCTTTG CTTCCACTGA GGACCGGGAG AGTTACCAGT CAGCCTTTAC GCCAGCCAAG 
CGACTTAAGA ACTGCCATGA CACAGAGTCT CCCCACTTGC GCTTCTCAGA TGCAGATGGC 
AAGGAATATG ACTTTGGGAC CCAGCTGCCA TCTAGCTCCC CTGGTTCACT TAAGGTTGAT 
GATGCTGGGA AGAAGATCTT TGCTGTCTCT GGCCTCATTT CGGATCGGGA AACCTCATCT 
AGCCCAGAGG ATCGGAATGA TAGATGTAAG AAAAAGGCAG CAGCATTGTT TGACAGCCAG 
GCCCCTATTT GCCCCATCTG CCAGGTCCTG CTGAGGCCCA GCGAGTTGCA GGAGCACATG 
GAGCAAGAGC TGGAACAGCT GGCCCAGCTG CCCACCAGCA AGAATTCCCT TCTGAAGGAT 
GCCATGGCTC CAGGTACCCC CAAGTCCCTC CTGTTGTCTG CTTCCATCAA GAGGGAAGGA 
GAGTCTCCGA CAGCATCACC ACACTCCTCC GCCACCGACG ACCTGCACCA CTCAGACAGA 
TACCAGTCTG CCTGTTCTGT GCTTTGTTTT TGGTTTCTCC ATGCAGCTCG GATTGGGAAA 
ATGAAACGGA GGAAGCAAGA TGAAGGGCAG AGGGAAGGCT CCTGCATGGC TGAGGATGAT 
GCCGTGGACA TCGAGCATGA GAACAGCAAC CGCTTTGAGG AGTATGAGTG GTGCGGGCAG 
AAGCGGATAC GGGCCACCAC TCTCCTGGAA GGTGGTTTCC GAGGCTCTGG CTTCGTCATG 
TGCAGCGGCA AAGAGAATCC GGACAGTGAT GCTGATTTGG ATGTGGATGG GGATGACACT 
CTGGAGTATG GGAAGCCACA ATACACGGAG GCTGACATCA TCCCGTGCAC AGGCGAGGAG 
CCTGGTGAAG CCAAGGAGAG AGAGGCCCTC CGGGGCGCAG TCCTAAATGG CGGCCCCCCC 
AGCACACGCA TCACACCTGA GTTTTCTAAA TGGGCCAGTG ATGAGATGCC ATCTACCAGC 
AATGGTGAGA GCAGCAAGCA GGAGGCCATG CAGAAGACCT GCAAGAATAG CGACATCGAA 
AAAATCACCG AAGATTCAGC TGTGACCACA TTTGAGGCCC TGAAGGCTCG GGTCAGGGAA 
CTTGAACGGC AGCTATCCCG TGGGGACCGT TACAAATGCC TCATCTGCAT GGACTCATAC 
TCGATGCCCC TAACGTCCAT CCAGTGTTGG CATGTGCACT GTGAGGAGTG CTGGCTGCGG 
ACCCTGGGTG CCAAGAAGCT CTGTCCTCAG TGCAACACCA TCACAGCGCC TGGAGACCTT 
CGGAGAATCT ACTTGTGA