cacna1bb gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021469036.1	XP_021324711.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_017352993.2	XP_017208482.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_017352996.1	XP_017208485.1	probable voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
XM_017352993.1	XP_017208482.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_017352995.1	XP_017208484.1	probable voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
XM_021473397.1	XP_021329072.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
XM_017352994.2	XP_017208483.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_017352991.1	XP_017208480.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_017352994.1	XP_017208483.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_021473398.1	XP_021329073.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
XM_017352992.2	XP_017208481.2	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_017352992.1	XP_017208481.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
XM_021473395.1	XP_021329070.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
XM_021473396.1	XP_021329071.1	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa56945	XM_017352996.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa56944	XM_017352995.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa146096	XM_021473395.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa146097	XM_021473396.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa146098	XM_021473397.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa146099	XM_021473398.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa151030	XM_021469036.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa151031	XM_017352992.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa151032	XM_017352993.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa151033	XM_017352994.2 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa56940	XM_017352991.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa56941	XM_017352992.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa56942	XM_017352993.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa56943	XM_017352994.1 Latest version!	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

cacna1bb ( XM_017352996.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208485.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa56945
Clone ID Related Accession (Same CDS sequence)	XM_017352996.1
Accession Version	XM_017352996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable voltage-dependent N-type calcium channel subunit alpha-1B isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC 
ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC 
TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC 
ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT 
CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG 
GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT 
GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC 
TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA 
TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC 
TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG 
CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC 
AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC 
ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT 
CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA 
TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG 
GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA 
CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT 
CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT 
TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT 
GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA 
GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC 
ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC 
GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC 
TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG 
AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC 
CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC 
AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC 
AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA 
GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC 
TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT 
TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC 
ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC 
TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG 
ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG 
CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG 
CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT 
GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC 
TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC 
GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA 
GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA 
GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208485.1
CDS	180..4004
Translation	MNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKTDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352996.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352996.1

ORF Insert Sequence:

ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC 
ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC 
TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC 
ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT 
CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG 
GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT 
GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC 
TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA 
TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC 
TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG 
CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC 
AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC 
ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT 
CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA 
TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG 
GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA 
CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT 
CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT 
TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT 
GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA 
GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC 
ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC 
GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC 
TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG 
AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC 
CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC 
AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC 
AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA 
GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC 
TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT 
TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC 
ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC 
TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG 
ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG 
CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG 
CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT 
GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC 
TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC 
GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA 
GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA 
GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_017352995.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208484.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa56944
Clone ID Related Accession (Same CDS sequence)	XM_017352995.1
Accession Version	XM_017352995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4740bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable voltage-dependent N-type calcium channel subunit alpha-1B isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCAGTT TTGTAAAGCA AAATCGAGAT GCAATCTCTC GCAGGTCTTC AGTCTGCAGC 
GTTCATTCAC CTAAAGAGCA GCAGAGGTCA CTGCAGAAGT TGTCCATATG GGAGCAGCGC 
ACTAACCAAC TGCGCAGACA CAACCTGCGT AGCAGCAGCG AGGCACTTTA CAACGAATTG 
GAGCCAGAAG AGCGTCTGCG TGTCTCCTCT GCCCTGCATC TGCGGCCTGA CATGAAGACC 
CACCACGATC GGCCGCTGGT TGTGGAGCAC CGAGATTCTG TGGACAATCT CAGATCTGAC 
AAAGACGGAG ACACTTCAGA GGGTCAGCCA GCATCTAACC ACCAGAGAAA ATATCATAAA 
TGTCGAGGGG AGAACGGAGA CACCGGCGAG GGACGGCTTC ACAGACACCA CAGCCAAAAT 
CGAGAGAACC AGGGTGGCCT AGAGCGCAGC AACAGTCAAG ATGGAGGACA GAGGAACCAC 
CACAGATCTT CTGGATCTCC AGAGGAGGGC ATTGGGATTG AGGAGAGAGA ACATAGACAT 
CATCATTCTA GAAGACCCAG AGAAGTCAAC GGGAACGGGA ATGGTGTGAT TGGTAATGGA 
GGCAGGGGCG AGAGGATACG AGGTCACAGA GAGGGTGAAG GAGGGAATGG AGAGAGAAGG 
AGACACCGGC CACGGGTTAA AGCCCAGTCC ACATTGGATG GAGACGAATG TAGAGAGAAT 
GGTGGTAAAC GCAGGAGCAG ACCAACAGAA ACAGACAGCC TCACAACCAG CCCTCTCCAG 
TCTCCTTCTG AAGAGAAGCC AGAAGACAAA GATAATCTAA AAAACAGCAC TCGAGTGGGT 
CCAAGCGGAG TCAACATCCC TGTCACCATA ACTGCGCCAC CAGGAGAGAC CACTGTCATT 
CCAATGAACA ACATTGATGG TGACAGTCTG CTGCTGAATG AGGAAAAGAA GGATCTTGAT 
GAGCTAAATC AGAATGCGCC TAAACACATT TTGCCATACA GCTCCATGTT TGTGTTTGGC 
CCAACCAACC CAGTGCGACG ACTGTGTCAT TATGTGGTGA ATCTGCGGTA TTTTGAGATG 
TGCATCCTCA CAGTCATCAC CATGAGCAGT ATCGCTCTCG CTGCCGAAGA CCCCGTGCAG 
GCTCATGCCC CTCGCAATGA TGTGCTGAAA TACCTTGATT ACGTCTTCAC TGGTGTGTTC 
ACATTTGAGA TGGTTATAAA GATGATTGAT CTTGGACTGC TGCTGCATCC CGGCTCTTAC 
TTCAGGGACC TGTGGAACAT CCTGGACTTC ATCGTAGTGA GCGGCGCTCT GGTGGCTTTC 
GCCTGCTCGA GCTTCATGGG AGGAACTAAA GGAAAGGACA TAAACACCAT CAAGTCCCTG 
AGGGTTCTTC GAGTCTTGAG ACCCTTGAAG ACAATCAAAA GATTGCCCAA ACTCAAGGCC 
GTGTTTGACT GCGTGGTGAA CTCTCTGAAG AATGTGCTCA ACATCCTCAT CGTCTACATC 
CTCTTCATGT TCATCTTCGC GGTCATCGCC GTGCAGCTTT TCAAGGGGAA GTTTTTCCAC 
TGCACCGATG AGTCCAAAGC CCTGGAGAAG GACTGCAGGG GTCAGTTCTT GGAGTACGGC 
AGTGATGGAG TGGCCATGAC GCAGCCGCGC GAGTGGAAGA AGTACGACTT CCATTATGAC 
AACGTCCTGT GGGCCTTTCT GACCCTCTTC ACTGTGTCTA CAGGAGAGGG CTGGCCACTT 
GTGCTGAAAC ACTCTGTTGA TGCCACGTAT GAAGACCAGG GTCCCAGTCC AGGATATCGC 
ATGGAAACCT CCATCTTCTA TGTGGTCTAC TTCATCGTCT TTCCCTTCTT CTTTGTCAAC 
ATCTTTGTGG CTTTGATCAT CATCACATTC CAGGAGCAGG GGGATAAAGC CATGTCCGAG 
TGCAGCTTGG AAAAGAACGA GCGAGCTTGT ATTGACTTTG CCATCAATGC TAAACCTCTG 
ACTCGCTACA TGCCGCAAGA CAAGAACTCT TACCAGTACA AGATGTGGAA GTTTGTGGTG 
TCGACTCCGT TTGAGTACTC CATCCTGACC ATGATCGCCA TCAATACAGT GGTGCTCATG 
ATGAAGTTCC ATGGTGCTCC TAAACCTTAT GAGGACATGT TGAAGTGGCT GAACATCATC 
TTCACTGCAC TCTTCACACT GGAGTGTGTT CTCAAGGTCA TCGCCTTCGG TCCTCTGAAC 
TACCTGAAAG AGGCTTGGAA CATTTTTGAC TTTGTTACTG TTCTGGGAAG CATCACAGAC 
ATCCTGGTTA CTGAGATCAA GACGGATAAG AGGATTAATC TGAGTTTCCT GAGACTCTTC 
CGGGCGGCTC GTCTCATTAA ACTCCTTCGG CAAGGATACA CCATTCGGAT CCTTCTCTGG 
ACCTTTGTTC AGTCCTTTAA GGCTCTTCCA TATGTGTGTC TCCTTATCGC AATGCTTTTT 
TTCATTTACG CCATCATCGG GATGCAGGTG TTTGGTAACA TCGCGTTGAA CAATGAAGAT 
TCGGCCATCA ACATTCACAA TAACTTCCGC ACCTTTTTTC AGGCCCTCAT GTTGTTGTTC 
AGAAGTGCTA CAGGGGAGGC TTGGCACGAG ATCATGTTGG CTTGTCTGAG TGAAAGATAT 
TGCGATATAG AATCTGGCTA CAGTGGGAAA GAGTGCGGCA GCGATTTTGC ATACTTCTAC 
TTCGTCTCCT TCATCTTTCT TTGCTCGTTT CTGATGCTGA ATCTGTTTGT GGCTGTCATC 
ATGGATAATT TTGAATATCT GACACGAGAT GCTTCTATTC TGGGGCCTCA TCACCTAGAT 
GAGTTCATCC GCGTCTGGGC TGAGTACGAC CCTGCCGCCT GTGGACGCAT GTCCTACCTC 
GATATGTACG AGATGCTCCG CCACATGTCC CCTCCGCTAG GTTTGGGAAA GAAATGTCCT 
CCACGAATCG CCTACAAGCG ATTGGTGAGG ATGAACATGC CCATAGCCGA GGACAACACG 
GTTCACTTTA CCTCCACTTT AATGGCTCTC ATCCGGACTG CCCTGGAGAT CAAACTGGCA 
TCAGGAGTTC TGGCTCAGCG CATTTGTGAT GCTGAATTAA GGAAGGAAAT CAATAAAATT 
TGGCCCAGTT TGTCATCTAA AACAGTAGAC CTGCTGGTTA CACCTCATAA ACATAACGAA 
TTGACTGTCG GGAAGGTCTA TGCAGCGCTC ATGATTTTTG ACTACTATAA GCAGAACAGA 
GCCAAAAGAC TCCAACAGCA GCAAGCTGCA CCAGGCACAC AGAGTAAAGT CGGTGTTCTC 
TTCAGGCCGA TGCTTCCTCT CACACACCTG CATGACCAGG AGCCTTTAGT GCCAAAAGGC 
CCGCCTCCTT CTCATTTAGA TATCACCCCT CAGTCAGATG CCACACCCAC TTCTTTTCAC 
TTGACCAATG GGGATGCAGT TCAAAGCCAG AGCAGTGCCA TGAAAAAGTC TCCTTCGGGA 
GGCCCATCTC AGGAAGTCAC ACAGGAAACG AACAGAAGGC CTATCCAACG TGGTCAATCG 
GAAGACGTGC CAAACACTTA CAGATCTCAG GAACTAGTAG AGATGACTGA CATGGAGAAT 
GATTCGGATG TTGGCGGGTA TCATGTGCTG GAGGGTCATG GCAGAGCTGC CTCTATGCCC 
AGACTGAATG CTGGCTTCCA GCGGAGCCAC TTACGCCACA CCGCCGGAGC GTATCTAGCA 
CCAATTGCAG ATGTGAGTCC CATGCGGCGA TCCACATCCT CGCTGACTCC ACAAAGAGGT 
GCGCGAGAGG TCAACCTGAG CGAATACGAC CTGGAGAGAC CCAGGATGAG CCCGAGCCAG 
ACTTTCGCTC AGGACAGAGA GAGAGAGAGA GTCCACCATC ATCATCACCA CCATCATCAT 
CGCTGTCAGA GACGCAGAGA CAAGAAGCAC AAGTCACTGG ATCGAGCCAT CAGCGAGGAG 
CAGCCGCCAC CTGCAGCTGA TCCAAACACA GAGTCGACGC CCAGGGAACG AGCCCGGGAG 
CGAGACCGTG GCCGCTCACA CGAGAGGAAG CACCACTCAT CCTCAGCAGC AGAAAGGAAG 
CAGCGATACT ACTCTTGTGA CCGCTACGGC AGCCATGAAC ATGGCCACAG CCTGTCCGCC 
GGGCCTAGCC GATCCACCTC TCCTGGAGAA CCACATGACA GGCTTAAACA GGGAATTAAC 
GCAGCTAAAG GAAGCTCAGT GGGTCATACC TCAGGCACCA GCACACCCTG CCGCGGGCGT 
AGACAGCTTC CCCAGACACC TCTTACCCCT CGGCCTGCTG TTGCCTATAA GACTGCCAAC 
TCTTCTCCGG TCCAGCTCAA CACTGCCCAA TCTACAGGGC CCTGCCCCGC TCGCCTTAGT 
CGTGGCCTGT CAGAGCACGA TACCTTACTC AGCGGTAGCA TCCACCGCCA ATCCCCTGTG 
CCTGTCACTC GTATTGGCTC TGACCCCAGC TTAGGTCCCC TCCAGCAAGA CTCGCACTTG 
TCTGAAGCGG AAGACTTTCA CGATGCCTTG AGCACGTACG GCACCGGTCG CTCCCCTAGA 
ACTACCGCCA TCATCGCCCA CACGTCATCT GGTGCGACCG CCACTTCTAC GCTGCTGTCC 
CGAAGCCAGA GCGGAGTCCC CAACGGGTAC CACTACTCCC TCGGAGTGAA CGCGGCTCCG 
GGATTGAGTG GGAGAGCGTC TGGAAGCTAC CAGGAGACTG AGAGAGATGA CTGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208484.1
CDS	52..4791
Translation	MCSFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKTDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352995.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352995.1

ORF Insert Sequence:

ATGTGCAGTT TTGTAAAGCA AAATCGAGAT GCAATCTCTC GCAGGTCTTC AGTCTGCAGC 
GTTCATTCAC CTAAAGAGCA GCAGAGGTCA CTGCAGAAGT TGTCCATATG GGAGCAGCGC 
ACTAACCAAC TGCGCAGACA CAACCTGCGT AGCAGCAGCG AGGCACTTTA CAACGAATTG 
GAGCCAGAAG AGCGTCTGCG TGTCTCCTCT GCCCTGCATC TGCGGCCTGA CATGAAGACC 
CACCACGATC GGCCGCTGGT TGTGGAGCAC CGAGATTCTG TGGACAATCT CAGATCTGAC 
AAAGACGGAG ACACTTCAGA GGGTCAGCCA GCATCTAACC ACCAGAGAAA ATATCATAAA 
TGTCGAGGGG AGAACGGAGA CACCGGCGAG GGACGGCTTC ACAGACACCA CAGCCAAAAT 
CGAGAGAACC AGGGTGGCCT AGAGCGCAGC AACAGTCAAG ATGGAGGACA GAGGAACCAC 
CACAGATCTT CTGGATCTCC AGAGGAGGGC ATTGGGATTG AGGAGAGAGA ACATAGACAT 
CATCATTCTA GAAGACCCAG AGAAGTCAAC GGGAACGGGA ATGGTGTGAT TGGTAATGGA 
GGCAGGGGCG AGAGGATACG AGGTCACAGA GAGGGTGAAG GAGGGAATGG AGAGAGAAGG 
AGACACCGGC CACGGGTTAA AGCCCAGTCC ACATTGGATG GAGACGAATG TAGAGAGAAT 
GGTGGTAAAC GCAGGAGCAG ACCAACAGAA ACAGACAGCC TCACAACCAG CCCTCTCCAG 
TCTCCTTCTG AAGAGAAGCC AGAAGACAAA GATAATCTAA AAAACAGCAC TCGAGTGGGT 
CCAAGCGGAG TCAACATCCC TGTCACCATA ACTGCGCCAC CAGGAGAGAC CACTGTCATT 
CCAATGAACA ACATTGATGG TGACAGTCTG CTGCTGAATG AGGAAAAGAA GGATCTTGAT 
GAGCTAAATC AGAATGCGCC TAAACACATT TTGCCATACA GCTCCATGTT TGTGTTTGGC 
CCAACCAACC CAGTGCGACG ACTGTGTCAT TATGTGGTGA ATCTGCGGTA TTTTGAGATG 
TGCATCCTCA CAGTCATCAC CATGAGCAGT ATCGCTCTCG CTGCCGAAGA CCCCGTGCAG 
GCTCATGCCC CTCGCAATGA TGTGCTGAAA TACCTTGATT ACGTCTTCAC TGGTGTGTTC 
ACATTTGAGA TGGTTATAAA GATGATTGAT CTTGGACTGC TGCTGCATCC CGGCTCTTAC 
TTCAGGGACC TGTGGAACAT CCTGGACTTC ATCGTAGTGA GCGGCGCTCT GGTGGCTTTC 
GCCTGCTCGA GCTTCATGGG AGGAACTAAA GGAAAGGACA TAAACACCAT CAAGTCCCTG 
AGGGTTCTTC GAGTCTTGAG ACCCTTGAAG ACAATCAAAA GATTGCCCAA ACTCAAGGCC 
GTGTTTGACT GCGTGGTGAA CTCTCTGAAG AATGTGCTCA ACATCCTCAT CGTCTACATC 
CTCTTCATGT TCATCTTCGC GGTCATCGCC GTGCAGCTTT TCAAGGGGAA GTTTTTCCAC 
TGCACCGATG AGTCCAAAGC CCTGGAGAAG GACTGCAGGG GTCAGTTCTT GGAGTACGGC 
AGTGATGGAG TGGCCATGAC GCAGCCGCGC GAGTGGAAGA AGTACGACTT CCATTATGAC 
AACGTCCTGT GGGCCTTTCT GACCCTCTTC ACTGTGTCTA CAGGAGAGGG CTGGCCACTT 
GTGCTGAAAC ACTCTGTTGA TGCCACGTAT GAAGACCAGG GTCCCAGTCC AGGATATCGC 
ATGGAAACCT CCATCTTCTA TGTGGTCTAC TTCATCGTCT TTCCCTTCTT CTTTGTCAAC 
ATCTTTGTGG CTTTGATCAT CATCACATTC CAGGAGCAGG GGGATAAAGC CATGTCCGAG 
TGCAGCTTGG AAAAGAACGA GCGAGCTTGT ATTGACTTTG CCATCAATGC TAAACCTCTG 
ACTCGCTACA TGCCGCAAGA CAAGAACTCT TACCAGTACA AGATGTGGAA GTTTGTGGTG 
TCGACTCCGT TTGAGTACTC CATCCTGACC ATGATCGCCA TCAATACAGT GGTGCTCATG 
ATGAAGTTCC ATGGTGCTCC TAAACCTTAT GAGGACATGT TGAAGTGGCT GAACATCATC 
TTCACTGCAC TCTTCACACT GGAGTGTGTT CTCAAGGTCA TCGCCTTCGG TCCTCTGAAC 
TACCTGAAAG AGGCTTGGAA CATTTTTGAC TTTGTTACTG TTCTGGGAAG CATCACAGAC 
ATCCTGGTTA CTGAGATCAA GACGGATAAG AGGATTAATC TGAGTTTCCT GAGACTCTTC 
CGGGCGGCTC GTCTCATTAA ACTCCTTCGG CAAGGATACA CCATTCGGAT CCTTCTCTGG 
ACCTTTGTTC AGTCCTTTAA GGCTCTTCCA TATGTGTGTC TCCTTATCGC AATGCTTTTT 
TTCATTTACG CCATCATCGG GATGCAGGTG TTTGGTAACA TCGCGTTGAA CAATGAAGAT 
TCGGCCATCA ACATTCACAA TAACTTCCGC ACCTTTTTTC AGGCCCTCAT GTTGTTGTTC 
AGAAGTGCTA CAGGGGAGGC TTGGCACGAG ATCATGTTGG CTTGTCTGAG TGAAAGATAT 
TGCGATATAG AATCTGGCTA CAGTGGGAAA GAGTGCGGCA GCGATTTTGC ATACTTCTAC 
TTCGTCTCCT TCATCTTTCT TTGCTCGTTT CTGATGCTGA ATCTGTTTGT GGCTGTCATC 
ATGGATAATT TTGAATATCT GACACGAGAT GCTTCTATTC TGGGGCCTCA TCACCTAGAT 
GAGTTCATCC GCGTCTGGGC TGAGTACGAC CCTGCCGCCT GTGGACGCAT GTCCTACCTC 
GATATGTACG AGATGCTCCG CCACATGTCC CCTCCGCTAG GTTTGGGAAA GAAATGTCCT 
CCACGAATCG CCTACAAGCG ATTGGTGAGG ATGAACATGC CCATAGCCGA GGACAACACG 
GTTCACTTTA CCTCCACTTT AATGGCTCTC ATCCGGACTG CCCTGGAGAT CAAACTGGCA 
TCAGGAGTTC TGGCTCAGCG CATTTGTGAT GCTGAATTAA GGAAGGAAAT CAATAAAATT 
TGGCCCAGTT TGTCATCTAA AACAGTAGAC CTGCTGGTTA CACCTCATAA ACATAACGAA 
TTGACTGTCG GGAAGGTCTA TGCAGCGCTC ATGATTTTTG ACTACTATAA GCAGAACAGA 
GCCAAAAGAC TCCAACAGCA GCAAGCTGCA CCAGGCACAC AGAGTAAAGT CGGTGTTCTC 
TTCAGGCCGA TGCTTCCTCT CACACACCTG CATGACCAGG AGCCTTTAGT GCCAAAAGGC 
CCGCCTCCTT CTCATTTAGA TATCACCCCT CAGTCAGATG CCACACCCAC TTCTTTTCAC 
TTGACCAATG GGGATGCAGT TCAAAGCCAG AGCAGTGCCA TGAAAAAGTC TCCTTCGGGA 
GGCCCATCTC AGGAAGTCAC ACAGGAAACG AACAGAAGGC CTATCCAACG TGGTCAATCG 
GAAGACGTGC CAAACACTTA CAGATCTCAG GAACTAGTAG AGATGACTGA CATGGAGAAT 
GATTCGGATG TTGGCGGGTA TCATGTGCTG GAGGGTCATG GCAGAGCTGC CTCTATGCCC 
AGACTGAATG CTGGCTTCCA GCGGAGCCAC TTACGCCACA CCGCCGGAGC GTATCTAGCA 
CCAATTGCAG ATGTGAGTCC CATGCGGCGA TCCACATCCT CGCTGACTCC ACAAAGAGGT 
GCGCGAGAGG TCAACCTGAG CGAATACGAC CTGGAGAGAC CCAGGATGAG CCCGAGCCAG 
ACTTTCGCTC AGGACAGAGA GAGAGAGAGA GTCCACCATC ATCATCACCA CCATCATCAT 
CGCTGTCAGA GACGCAGAGA CAAGAAGCAC AAGTCACTGG ATCGAGCCAT CAGCGAGGAG 
CAGCCGCCAC CTGCAGCTGA TCCAAACACA GAGTCGACGC CCAGGGAACG AGCCCGGGAG 
CGAGACCGTG GCCGCTCACA CGAGAGGAAG CACCACTCAT CCTCAGCAGC AGAAAGGAAG 
CAGCGATACT ACTCTTGTGA CCGCTACGGC AGCCATGAAC ATGGCCACAG CCTGTCCGCC 
GGGCCTAGCC GATCCACCTC TCCTGGAGAA CCACATGACA GGCTTAAACA GGGAATTAAC 
GCAGCTAAAG GAAGCTCAGT GGGTCATACC TCAGGCACCA GCACACCCTG CCGCGGGCGT 
AGACAGCTTC CCCAGACACC TCTTACCCCT CGGCCTGCTG TTGCCTATAA GACTGCCAAC 
TCTTCTCCGG TCCAGCTCAA CACTGCCCAA TCTACAGGGC CCTGCCCCGC TCGCCTTAGT 
CGTGGCCTGT CAGAGCACGA TACCTTACTC AGCGGTAGCA TCCACCGCCA ATCCCCTGTG 
CCTGTCACTC GTATTGGCTC TGACCCCAGC TTAGGTCCCC TCCAGCAAGA CTCGCACTTG 
TCTGAAGCGG AAGACTTTCA CGATGCCTTG AGCACGTACG GCACCGGTCG CTCCCCTAGA 
ACTACCGCCA TCATCGCCCA CACGTCATCT GGTGCGACCG CCACTTCTAC GCTGCTGTCC 
CGAAGCCAGA GCGGAGTCCC CAACGGGTAC CACTACTCCC TCGGAGTGAA CGCGGCTCCG 
GGATTGAGTG GGAGAGCGTC TGGAAGCTAC CAGGAGACTG AGAGAGATGA CTGGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_021473395.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329070.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

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CloneID	ODa146096
Clone ID Related Accession (Same CDS sequence)	XM_021473395.1
Accession Version	XM_021473395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCGAGCT TCATGGGATC GCAGCAAAGC GTGACCAGAG GAACTAAAGG AAAGGACATA 
AACACCATCA AGTCCCTGAG GGTTCTTCGA GTCTTGAGAC CCTTGAAGAC AATCAAAAGA 
TTGCCCAAAC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTGAAGAA TGTGCTCAAC 
ATCCTCATCG TCTACATCCT CTTCATGTTC ATCTTCGCGG TCATCGCCGT GCAGCTTTTC 
AAGGGGAAGT TTTTCCACTG CACCGATGAG TCCAAAGCCC TGGAGAAGGA CTGCAGGGGT 
CAGTTCTTGG AGTACGGCAG TGATGGAGTG GCCATGACGC AGCCGCGCGA GTGGAAGAAG 
TACGACTTCC ATTATGACAA CGTCCTGTGG GCCTTTCTGA CCCTCTTCAC TGTGTCTACA 
GGAGAGGGCT GGCCACTTGT GCTGAAACAC TCTGTTGATG CCACGTATGA AGACCAGGGT 
CCCAGTCCAG GATATCGCAT GGAAACCTCC ATCTTCTATG TGGTCTACTT CATCGTCTTT 
CCCTTCTTCT TTGTCAACAT CTTTGTGGCT TTGATCATCA TCACATTCCA GGAGCAGGGG 
GATAAAGCCA TGTCCGAGTG CAGCTTGGAA AAGAACGAGC GAGCTTGTAT TGACTTTGCC 
ATCAATGCTA AACCTCTGAC TCGCTACATG CCGCAAGACA AGAACTCTTA CCAGTACAAG 
ATGTGGAAGT TTGTGGTGTC GACTCCGTTT GAGTACTCCA TCCTGACCAT GATCGCCATC 
AATACAGTGG TGCTCATGAT GAAGTTCCAT GGTGCTCCTA AACCTTATGA GGACATGTTG 
AAGTGGCTGA ACATCATCTT CACTGCACTC TTCACACTGG AGTGTGTTCT CAAGGTCATC 
GCCTTCGGTC CTCTGAACTA CCTGAAAGAG GCTTGGAACA TTTTTGACTT TGTTACTGTT 
CTGGGAAGCA TCACAGACAT CCTGGTTACT GAGATCAAGA CGGATAAGAG GATTAATCTG 
AGTTTCCTGA GACTCTTCCG GGCGGCTCGT CTCATTAAAC TCCTTCGGCA AGGATACACC 
ATTCGGATCC TTCTCTGGAC CTTTGTTCAG TCCTTTAAGG CTCTTCCATA TGTGTGTCTC 
CTTATCGCAA TGCTTTTTTT CATTTACGCC ATCATCGGGA TGCAGGTGTT TGGTAACATC 
GCGTTGAACA ATGAAGATTC GGCCATCAAC ATTCACAATA ACTTCCGCAC CTTTTTTCAG 
GCCCTCATGT TGTTGTTCAG AAGTGCTACA GGGGAGGCTT GGCACGAGAT CATGTTGGCT 
TGTCTGAGTG AAAGATATTG CGATATAGAA TCTGGCTACA GTGGGAAAGA GTGCGGCAGC 
GATTTTGCAT ACTTCTACTT CGTCTCCTTC ATCTTTCTTT GCTCGTTTCT GATGCTGAAT 
CTGTTTGTGG CTGTCATCAT GGATAATTTT GAATATCTGA CACGAGATGC TTCTATTCTG 
GGGCCTCATC ACCTAGATGA GTTCATCCGC GTCTGGGCTG AGTACGACCC TGCCGCCTGT 
GGACGCATGT CCTACCTCGA TATGTACGAG ATGCTCCGCC ACATGTCCCC TCCGCTAGGT 
TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGAT TGGTGAGGAT GAACATGCCC 
ATAGCCGAGG ACAACACGGT TCACTTTACC TCCACTTTAA TGGCTCTCAT CCGGACTGCC 
CTGGAGATCA AACTGGCATC AGGAGTTCTG GCTCAGCGCA TTTGTGATGC TGAATTAAGG 
AAGGAAATCA ATAAAATTTG GCCCAGTTTG TCATCTAAAA CAGTAGACCT GCTGGTTACA 
CCTCATAAAC ATAACGAATT GACTGTCGGG AAGGTCTATG CAGCGCTCAT GATTTTTGAC 
TACTATAAGC AGAACAGAGC CAAAAGACTC CAACAGCAGC AAGCTGCACC AGGCACACAG 
AGTAAAGTCG GTGTTCTCTT CAGGCCGATG CTTCCTCTCA CACACCTGCA TGACCAGGAG 
CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT CATTTAGATA TCACCCCTCA GTCAGATGCC 
ACACCCACTT CTTTTCACTT GACCAATGGG GATGCAGTTC AAAGCCAGAG CAGTGCCATG 
AAAAAGTCTC CTTCGGGAGG CCCATCTCAG GAAGTCACAC AGGAAACGAA CAGAAGGCCT 
ATCCAACGTG GTCAATCGGA AGACGTGCCA AACACTTACA GATCTCAGGA ACTAGTAGAG 
ATGACTGACA TGGAGAATGA TTCGGATGTT GGCGGGTATC ATGTGCTGGA GGGTCATGGC 
AGAGCTGCCT CTATGCCCAG ACTGAATGCT GGCTTCCAGC GGAGCCACTT ACGCCACACC 
GCCGGAGCGT ATCTAGCACC AATTGCAGAT GTGAGTCCCA TGCGGCGATC CACATCCTCG 
CTGACTCCAC AAAGAGGTGC GCGAGAGGTC AACCTGAGCG AATACGACCT GGAGAGACCC 
AGGATGAGCC CGAGCCAGAC TTTCGCTCAG GACAGAGAGA GAGAGAGAGT CCACCATCAT 
CATCACCACC ATCATCATCG CTGTCAGAGA CGCAGAGACA AGAAGCACAA GTCACTGGAT 
CGAGCCATCA GCGAGGAGCA GCCGCCACCT GCAGCTGATC CAAACACAGA GTCGACGCCC 
AGGGAACGAG CCCGGGAGCG AGACCGTGGC CGCTCACACG AGAGGAAGCA CCACTCATCC 
TCAGCAGCAG AAAGGAAGCA GCGATACTAC TCTTGTGACC GCTACGGCAG CCATGAACAT 
GGCCACAGCC TGTCCGCCGG GCCTAGCCGA TCCACCTCTC CTGGAGAACC ACATGACAGG 
CTTAAACAGG GAATTAACGC AGCTAAAGGA AGCTCAGTGG GTCATACCTC AGGCACCAGC 
ACACCCTGCC GCGGGCGTAG ACAGCTTCCC CAGACACCTC TTACCCCTCG GCCTGCTGTT 
GCCTATAAGA CTGCCAACTC TTCTCCGGTC CAGCTCAACA CTGCCCAATC TACAGGGCCC 
TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA GAGCACGATA CCTTACTCAG CGGTAGCATC 
CACCGCCAAT CCCCTGTGCC TGTCACTCGT ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC 
CAGCAAGACT CGCACTTGTC TGAAGCGGAA GACTTTCACG ATGCCTTGAG CACGTACGGC 
ACCGGTCGCT CCCCTAGAAC TACCGCCATC ATCGCCCACA CGTCATCTGG TGCGACCGCC 
ACTTCTACGC TGCTGTCCCG AAGCCAGAGC GGAGTCCCCA ACGGGTACCA CTACTCCCTC 
GGAGTGAACG CGGCTCCGGG ATTGAGTGGG AGAGCGTCTG GAAGCTACCA GGAGACTGAG 
AGAGATGACT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329070.1
CDS	565..7602
Translation	MSRMARFGDDLPTRYGGPAGAGRGGARAGGPSGGQRMYKQSMAQRARTMALYNPKQTKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYLILTTIIANCIVLALEQHLPALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSSFMGSQQSVTRGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKTDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_021473395.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473395.1

ORF Insert Sequence:

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCGAGCT TCATGGGATC GCAGCAAAGC GTGACCAGAG GAACTAAAGG AAAGGACATA 
AACACCATCA AGTCCCTGAG GGTTCTTCGA GTCTTGAGAC CCTTGAAGAC AATCAAAAGA 
TTGCCCAAAC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTGAAGAA TGTGCTCAAC 
ATCCTCATCG TCTACATCCT CTTCATGTTC ATCTTCGCGG TCATCGCCGT GCAGCTTTTC 
AAGGGGAAGT TTTTCCACTG CACCGATGAG TCCAAAGCCC TGGAGAAGGA CTGCAGGGGT 
CAGTTCTTGG AGTACGGCAG TGATGGAGTG GCCATGACGC AGCCGCGCGA GTGGAAGAAG 
TACGACTTCC ATTATGACAA CGTCCTGTGG GCCTTTCTGA CCCTCTTCAC TGTGTCTACA 
GGAGAGGGCT GGCCACTTGT GCTGAAACAC TCTGTTGATG CCACGTATGA AGACCAGGGT 
CCCAGTCCAG GATATCGCAT GGAAACCTCC ATCTTCTATG TGGTCTACTT CATCGTCTTT 
CCCTTCTTCT TTGTCAACAT CTTTGTGGCT TTGATCATCA TCACATTCCA GGAGCAGGGG 
GATAAAGCCA TGTCCGAGTG CAGCTTGGAA AAGAACGAGC GAGCTTGTAT TGACTTTGCC 
ATCAATGCTA AACCTCTGAC TCGCTACATG CCGCAAGACA AGAACTCTTA CCAGTACAAG 
ATGTGGAAGT TTGTGGTGTC GACTCCGTTT GAGTACTCCA TCCTGACCAT GATCGCCATC 
AATACAGTGG TGCTCATGAT GAAGTTCCAT GGTGCTCCTA AACCTTATGA GGACATGTTG 
AAGTGGCTGA ACATCATCTT CACTGCACTC TTCACACTGG AGTGTGTTCT CAAGGTCATC 
GCCTTCGGTC CTCTGAACTA CCTGAAAGAG GCTTGGAACA TTTTTGACTT TGTTACTGTT 
CTGGGAAGCA TCACAGACAT CCTGGTTACT GAGATCAAGA CGGATAAGAG GATTAATCTG 
AGTTTCCTGA GACTCTTCCG GGCGGCTCGT CTCATTAAAC TCCTTCGGCA AGGATACACC 
ATTCGGATCC TTCTCTGGAC CTTTGTTCAG TCCTTTAAGG CTCTTCCATA TGTGTGTCTC 
CTTATCGCAA TGCTTTTTTT CATTTACGCC ATCATCGGGA TGCAGGTGTT TGGTAACATC 
GCGTTGAACA ATGAAGATTC GGCCATCAAC ATTCACAATA ACTTCCGCAC CTTTTTTCAG 
GCCCTCATGT TGTTGTTCAG AAGTGCTACA GGGGAGGCTT GGCACGAGAT CATGTTGGCT 
TGTCTGAGTG AAAGATATTG CGATATAGAA TCTGGCTACA GTGGGAAAGA GTGCGGCAGC 
GATTTTGCAT ACTTCTACTT CGTCTCCTTC ATCTTTCTTT GCTCGTTTCT GATGCTGAAT 
CTGTTTGTGG CTGTCATCAT GGATAATTTT GAATATCTGA CACGAGATGC TTCTATTCTG 
GGGCCTCATC ACCTAGATGA GTTCATCCGC GTCTGGGCTG AGTACGACCC TGCCGCCTGT 
GGACGCATGT CCTACCTCGA TATGTACGAG ATGCTCCGCC ACATGTCCCC TCCGCTAGGT 
TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGAT TGGTGAGGAT GAACATGCCC 
ATAGCCGAGG ACAACACGGT TCACTTTACC TCCACTTTAA TGGCTCTCAT CCGGACTGCC 
CTGGAGATCA AACTGGCATC AGGAGTTCTG GCTCAGCGCA TTTGTGATGC TGAATTAAGG 
AAGGAAATCA ATAAAATTTG GCCCAGTTTG TCATCTAAAA CAGTAGACCT GCTGGTTACA 
CCTCATAAAC ATAACGAATT GACTGTCGGG AAGGTCTATG CAGCGCTCAT GATTTTTGAC 
TACTATAAGC AGAACAGAGC CAAAAGACTC CAACAGCAGC AAGCTGCACC AGGCACACAG 
AGTAAAGTCG GTGTTCTCTT CAGGCCGATG CTTCCTCTCA CACACCTGCA TGACCAGGAG 
CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT CATTTAGATA TCACCCCTCA GTCAGATGCC 
ACACCCACTT CTTTTCACTT GACCAATGGG GATGCAGTTC AAAGCCAGAG CAGTGCCATG 
AAAAAGTCTC CTTCGGGAGG CCCATCTCAG GAAGTCACAC AGGAAACGAA CAGAAGGCCT 
ATCCAACGTG GTCAATCGGA AGACGTGCCA AACACTTACA GATCTCAGGA ACTAGTAGAG 
ATGACTGACA TGGAGAATGA TTCGGATGTT GGCGGGTATC ATGTGCTGGA GGGTCATGGC 
AGAGCTGCCT CTATGCCCAG ACTGAATGCT GGCTTCCAGC GGAGCCACTT ACGCCACACC 
GCCGGAGCGT ATCTAGCACC AATTGCAGAT GTGAGTCCCA TGCGGCGATC CACATCCTCG 
CTGACTCCAC AAAGAGGTGC GCGAGAGGTC AACCTGAGCG AATACGACCT GGAGAGACCC 
AGGATGAGCC CGAGCCAGAC TTTCGCTCAG GACAGAGAGA GAGAGAGAGT CCACCATCAT 
CATCACCACC ATCATCATCG CTGTCAGAGA CGCAGAGACA AGAAGCACAA GTCACTGGAT 
CGAGCCATCA GCGAGGAGCA GCCGCCACCT GCAGCTGATC CAAACACAGA GTCGACGCCC 
AGGGAACGAG CCCGGGAGCG AGACCGTGGC CGCTCACACG AGAGGAAGCA CCACTCATCC 
TCAGCAGCAG AAAGGAAGCA GCGATACTAC TCTTGTGACC GCTACGGCAG CCATGAACAT 
GGCCACAGCC TGTCCGCCGG GCCTAGCCGA TCCACCTCTC CTGGAGAACC ACATGACAGG 
CTTAAACAGG GAATTAACGC AGCTAAAGGA AGCTCAGTGG GTCATACCTC AGGCACCAGC 
ACACCCTGCC GCGGGCGTAG ACAGCTTCCC CAGACACCTC TTACCCCTCG GCCTGCTGTT 
GCCTATAAGA CTGCCAACTC TTCTCCGGTC CAGCTCAACA CTGCCCAATC TACAGGGCCC 
TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA GAGCACGATA CCTTACTCAG CGGTAGCATC 
CACCGCCAAT CCCCTGTGCC TGTCACTCGT ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC 
CAGCAAGACT CGCACTTGTC TGAAGCGGAA GACTTTCACG ATGCCTTGAG CACGTACGGC 
ACCGGTCGCT CCCCTAGAAC TACCGCCATC ATCGCCCACA CGTCATCTGG TGCGACCGCC 
ACTTCTACGC TGCTGTCCCG AAGCCAGAGC GGAGTCCCCA ACGGGTACCA CTACTCCCTC 
GGAGTGAACG CGGCTCCGGG ATTGAGTGGG AGAGCGTCTG GAAGCTACCA GGAGACTGAG 
AGAGATGACT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_021473396.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329071.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

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CloneID	ODa146097
Clone ID Related Accession (Same CDS sequence)	XM_021473396.1
Accession Version	XM_021473396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC 
ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC 
TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC 
ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT 
CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG 
GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT 
GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC 
TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA 
TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC 
TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG 
CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC 
AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC 
ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT 
CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA 
TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG 
GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA 
CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT 
CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT 
TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT 
GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA 
GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC 
ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC 
GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC 
TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG 
AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC 
CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC 
AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC 
AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA 
GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC 
TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT 
TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC 
ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC 
TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG 
ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG 
CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG 
CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT 
GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC 
TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC 
GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA 
GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA 
GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329071.1
CDS	565..7569
Translation	MSRMARFGDDLPTRYGGPAGAGRGGARAGGPSGGQRMYKQSMAQRARTMALYNPKQTKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYLILTTIIANCIVLALEQHLPALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKTDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_021473396.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473396.1

ORF Insert Sequence:

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC 
ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC 
TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC 
ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT 
CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG 
GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT 
GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC 
TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA 
TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC 
TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG 
CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC 
AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC 
ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT 
CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA 
TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG 
GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA 
CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT 
CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT 
TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT 
GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA 
GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC 
ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC 
GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC 
TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG 
AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC 
CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC 
AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC 
AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA 
GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC 
TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT 
TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC 
ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC 
TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG 
ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG 
CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG 
CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT 
GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC 
TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC 
GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA 
GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA 
GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_021473397.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329072.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

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CloneID	ODa146098
Clone ID Related Accession (Same CDS sequence)	XM_021473397.1
Accession Version	XM_021473397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT 
AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT 
AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC 
GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC 
AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG 
GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC 
TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT 
CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT 
CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG 
GCTGAGTACG ACCCTGCCGC CTGTGGACGC ATGTCCTACC TCGATATGTA CGAGATGCTC 
CGCCACATGT CCCCTCCGCT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT 
TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG 
CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT 
AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC 
TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG 
CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT 
CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA 
GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA 
GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC 
ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT 
TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG 
TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC 
CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT 
CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG 
AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA 
GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA 
GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT 
GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA 
CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT 
GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC 
TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA 
GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA 
CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC 
AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC 
GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC 
TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT 
CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC 
CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC 
CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG 
TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329072.1
CDS	566..7567
Translation	MSRMARFGDDLPTRYGGPAGAGRGGARAGGPSGGQRMYKQSMAQRARTMALYNPKQTKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYLILTTIIANCIVLALEQHLPALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_021473397.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473397.1

ORF Insert Sequence:

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT 
AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT 
AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC 
GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC 
AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG 
GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC 
TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT 
CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT 
CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG 
GCTGAGTACG ACCCTGCCGC CTGTGGACGC ATGTCCTACC TCGATATGTA CGAGATGCTC 
CGCCACATGT CCCCTCCGCT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT 
TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG 
CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT 
AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC 
TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG 
CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT 
CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA 
GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA 
GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC 
ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT 
TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG 
TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC 
CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT 
CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG 
AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA 
GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA 
GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT 
GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA 
CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT 
GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC 
TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA 
GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA 
CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC 
AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC 
GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC 
TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT 
CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC 
CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC 
CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG 
TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_021473398.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329073.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

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CloneID	ODa146099
Clone ID Related Accession (Same CDS sequence)	XM_021473398.1
Accession Version	XM_021473398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395080.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT 
AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT 
AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC 
GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC 
AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG 
GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC 
TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT 
CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT 
CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG 
GCTGAGTACG ACCCTGCCGC CTGTGGAAGG ATTCCCTATA TGGATATGTA CAATTTGTTG 
CGTGTAATCT CACCTCCCCT TGGCTTAGGA AAGAACTGCC CTAATAGGGT GGCATACAAG 
CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT 
TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG 
CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT 
AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC 
TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG 
CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT 
CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA 
GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA 
GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC 
ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT 
TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG 
TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC 
CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT 
CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG 
AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA 
GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA 
GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT 
GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA 
CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT 
GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC 
TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA 
GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA 
CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC 
AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC 
GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC 
TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT 
CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC 
CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC 
CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG 
TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329073.1
CDS	566..7567
Translation	MSRMARFGDDLPTRYGGPAGAGRGGARAGGPSGGQRMYKQSMAQRARTMALYNPKQTKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYLILTTIIANCIVLALEQHLPALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRIPYMDMYNLLRVISPPLGLGKNCPNRVAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_021473398.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473398.1

ORF Insert Sequence:

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATCG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTTGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTCATCCCT CTCATCATCA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT 
AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT 
AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC 
GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC 
AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG 
GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC 
TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT 
CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT 
CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG 
GCTGAGTACG ACCCTGCCGC CTGTGGAAGG ATTCCCTATA TGGATATGTA CAATTTGTTG 
CGTGTAATCT CACCTCCCCT TGGCTTAGGA AAGAACTGCC CTAATAGGGT GGCATACAAG 
CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT 
TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG 
CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT 
AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC 
TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG 
CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT 
CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA 
GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA 
GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC 
ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT 
TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG 
TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC 
CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT 
CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG 
AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA 
GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA 
GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT 
GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA 
CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT 
GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC 
TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA 
GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA 
CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC 
AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC 
GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC 
TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT 
CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC 
CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC 
CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG 
TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_021469036.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324711.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

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CloneID	ODa151030
Clone ID Related Accession (Same CDS sequence)	XM_021469036.1
Accession Version	XM_021469036.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7038bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCGAGCT TCATGGGATC GCAGCAAAGC GTGACCAGAG GAACTAAAGG AAAGGACATA 
AACACCATCA AGTCCCTGAG GGTTCTTCGA GTCTTGAGAC CCTTGAAGAC AATCAAAAGA 
TTGCCCAAAC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTGAAGAA TGTGCTCAAC 
ATCCTCATCG TCTACATCCT CTTCATGTTC ATCTTCGCGG TCATCGCCGT GCAGCTTTTC 
AAGGGGAAGT TTTTCCACTG CACCGATGAG TCCAAAGCCC TGGAGAAGGA CTGCAGGGGT 
CAGTTCTTGG AGTACGGCAG TGATGGAGTG GCCATGACGC AGCCGCGCGA GTGGAAGAAG 
TACGACTTCC ATTATGACAA CGTCCTGTGG GCCTTTCTGA CCCTCTTCAC TGTGTCTACA 
GGAGAGGGCT GGCCACTTGT GCTGAAACAC TCTGTTGATG CCACGTATGA AGACCAGGGT 
CCCAGTCCAG GATATCGCAT GGAAACCTCC ATCTTCTATG TGGTCTACTT CATCGTCTTT 
CCCTTCTTCT TTGTCAACAT CTTTGTGGCT TTGATCATCA TCACATTCCA GGAGCAGGGG 
GATAAAGCCA TGTCCGAGTG CAGCTTGGAA AAGAACGAGC GAGCTTGTAT TGACTTTGCC 
ATCAATGCTA AACCTCTGAC TCGCTACATG CCGCAAGACA AGAACTCTTA CCAGTACAAG 
ATGTGGAAGT TTGTGGTGTC GACTCCGTTT GAGTACTCCA TCCTGACCAT GATCGCCATC 
AATACAGTGG TGCTCATGAT GAAGTTCCAT GGTGCTCCTA AACCTTATGA GGACATGTTG 
AAGTGGCTGA ACATCATCTT CACTGCACTC TTCACACTGG AGTGTGTTCT CAAGGTCATC 
GCCTTCGGTC CTCTGAACTA CCTGAAAGAG GCTTGGAACA TTTTTGACTT TGTTACTGTT 
CTGGGAAGCA TCACAGACAT CCTGGTTACT GAGATCAAGA CGGATAAGAG GATTAATCTG 
AGTTTCCTGA GACTCTTCCG GGCGGCTCGT CTCATTAAAC TCCTTCGGCA AGGATACACC 
ATTCGGATCC TTCTCTGGAC CTTTGTTCAG TCCTTTAAGG CTCTTCCATA TGTGTGTCTC 
CTTATCGCAA TGCTTTTTTT CATTTACGCC ATCATCGGGA TGCAGGTGTT TGGTAACATC 
GCGTTGAACA ATGAAGATTC GGCCATCAAC ATTCACAATA ACTTCCGCAC CTTTTTTCAG 
GCCCTCATGT TGTTGTTCAG AAGTGCTACA GGGGAGGCTT GGCACGAGAT CATGTTGGCT 
TGTCTGAGTG AAAGATATTG CGATATAGAA TCTGGCTACA GTGGGAAAGA GTGCGGCAGC 
GATTTTGCAT ACTTCTACTT CGTCTCCTTC ATCTTTCTTT GCTCGTTTCT GATGCTGAAT 
CTGTTTGTGG CTGTCATCAT GGATAATTTT GAATATCTGA CACGAGATGC TTCTATTCTG 
GGGCCTCATC ACCTAGATGA GTTCATCCGC GTCTGGGCTG AGTACGACCC TGCCGCCTGT 
GGACGCATGT CCTACCTCGA TATGTACGAG ATGCTCCGCC ACATGTCCCC TCCGCTAGGT 
TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGAT TGGTGAGGAT GAACATGCCC 
ATAGCCGAGG ACAACACGGT TCACTTTACC TCCACTTTAA TGGCTCTCAT CCGGACTGCC 
CTGGAGATCA AACTGGCATC AGGAGTTCTG GCTCAGCGCA TTTGTGATGC TGAATTAAGG 
AAGGAAATCA ATAAAATTTG GCCCAGTTTG TCATCTAAAA CAGTAGACCT GCTGGTTACA 
CCTCATAAAC ATAACGAATT GACTGTCGGG AAGGTCTATG CAGCGCTCAT GATTTTTGAC 
TACTATAAGC AGAACAGAGC CAAAAGACTC CAACAGCAGC AAGCTGCACC AGGCACACAG 
AGTAAAGTCG GTGTTCTCTT CAGGCCGATG CTTCCTCTCA CACACCTGCA TGACCAGGAG 
CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT CATTTAGATA TCACCCCTCA GTCAGATGCC 
ACACCCACTT CTTTTCACTT GACCAATGGG GATGCAGTTC AAAGCCAGAG CAGTGCCATG 
AAAAAGTCTC CTTCGGGAGG CCCATCTCAG GAAGTCACAC AGGAAACGAA CAGAAGGCCT 
ATCCAACGTG GTCAATCGGA AGACGTGCCA AACACTTACA GATCTCAGGA ACTAGTAGAG 
ATGACTGACA TGGAGAATGA TTCGGATGTT GGCGGGTATC ATGTGCTGGA GGGTCATGGC 
AGAGCTGCCT CTATGCCCAG ACTGAATGCT GGCTTCCAGC GGAGCCACTT ACGCCACACC 
GCCGGAGCGT ATCTAGCACC AATTGCAGAT GTGAGTCCCA TGCGGCGATC CACATCCTCG 
CTGACTCCAC AAAGAGGTGC GCGAGAGGTC AACCTGAGCG AATACGACCT GGAGAGACCC 
AGGATGAGCC CGAGCCAGAC TTTCGCTCAG GACAGAGAGA GAGAGAGAGT CCACCATCAT 
CATCACCACC ATCATCATCG CTGTCAGAGA CGCAGAGACA AGAAGCACAA GTCACTGGAT 
CGAGCCATCA GCGAGGAGCA GCCGCCACCT GCAGCTGATC CAAACACAGA GTCGACGCCC 
AGGGAACGAG CCCGGGAGCG AGACCGTGGC CGCTCACACG AGAGGAAGCA CCACTCATCC 
TCAGCAGCAG AAAGGAAGCA GCGATACTAC TCTTGTGACC GCTACGGCAG CCATGAACAT 
GGCCACAGCC TGTCCGCCGG GCCTAGCCGA TCCACCTCTC CTGGAGAACC ACATGACAGG 
CTTAAACAGG GAATTAACGC AGCTAAAGGA AGCTCAGTGG GTCATACCTC AGGCACCAGC 
ACACCCTGCC GCGGGCGTAG ACAGCTTCCC CAGACACCTC TTACCCCTCG GCCTGCTGTT 
GCCTATAAGA CTGCCAACTC TTCTCCGGTC CAGCTCAACA CTGCCCAATC TACAGGGCCC 
TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA GAGCACGATA CCTTACTCAG CGGTAGCATC 
CACCGCCAAT CCCCTGTGCC TGTCACTCGT ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC 
CAGCAAGACT CGCACTTGTC TGAAGCGGAA GACTTTCACG ATGCCTTGAG CACGTACGGC 
ACCGGTCGCT CCCCTAGAAC TACCGCCATC ATCGCCCACA CGTCATCTGG TGCGACCGCC 
ACTTCTACGC TGCTGTCCCG AAGCCAGAGC GGAGTCCCCA ACGGGTACCA CTACTCCCTC 
GGAGTGAACG CGGCTCCGGG ATTGAGTGGG AGAGCGTCTG GAAGCTACCA GGAGACTGAG 
AGAGATGACT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324711.1
CDS	556..7593
Translation	MSRMARFGDDLPTRYGGPAGAGRGGARAGGPSGGQRMYKQSMAQRARTMALYNPKQTKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYLILTTIIANCIVLALEQHLPALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSSFMGSQQSVTRGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKTDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_021469036.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469036.1

ORF Insert Sequence:

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCGAGCT TCATGGGATC GCAGCAAAGC GTGACCAGAG GAACTAAAGG AAAGGACATA 
AACACCATCA AGTCCCTGAG GGTTCTTCGA GTCTTGAGAC CCTTGAAGAC AATCAAAAGA 
TTGCCCAAAC TCAAGGCCGT GTTTGACTGC GTGGTGAACT CTCTGAAGAA TGTGCTCAAC 
ATCCTCATCG TCTACATCCT CTTCATGTTC ATCTTCGCGG TCATCGCCGT GCAGCTTTTC 
AAGGGGAAGT TTTTCCACTG CACCGATGAG TCCAAAGCCC TGGAGAAGGA CTGCAGGGGT 
CAGTTCTTGG AGTACGGCAG TGATGGAGTG GCCATGACGC AGCCGCGCGA GTGGAAGAAG 
TACGACTTCC ATTATGACAA CGTCCTGTGG GCCTTTCTGA CCCTCTTCAC TGTGTCTACA 
GGAGAGGGCT GGCCACTTGT GCTGAAACAC TCTGTTGATG CCACGTATGA AGACCAGGGT 
CCCAGTCCAG GATATCGCAT GGAAACCTCC ATCTTCTATG TGGTCTACTT CATCGTCTTT 
CCCTTCTTCT TTGTCAACAT CTTTGTGGCT TTGATCATCA TCACATTCCA GGAGCAGGGG 
GATAAAGCCA TGTCCGAGTG CAGCTTGGAA AAGAACGAGC GAGCTTGTAT TGACTTTGCC 
ATCAATGCTA AACCTCTGAC TCGCTACATG CCGCAAGACA AGAACTCTTA CCAGTACAAG 
ATGTGGAAGT TTGTGGTGTC GACTCCGTTT GAGTACTCCA TCCTGACCAT GATCGCCATC 
AATACAGTGG TGCTCATGAT GAAGTTCCAT GGTGCTCCTA AACCTTATGA GGACATGTTG 
AAGTGGCTGA ACATCATCTT CACTGCACTC TTCACACTGG AGTGTGTTCT CAAGGTCATC 
GCCTTCGGTC CTCTGAACTA CCTGAAAGAG GCTTGGAACA TTTTTGACTT TGTTACTGTT 
CTGGGAAGCA TCACAGACAT CCTGGTTACT GAGATCAAGA CGGATAAGAG GATTAATCTG 
AGTTTCCTGA GACTCTTCCG GGCGGCTCGT CTCATTAAAC TCCTTCGGCA AGGATACACC 
ATTCGGATCC TTCTCTGGAC CTTTGTTCAG TCCTTTAAGG CTCTTCCATA TGTGTGTCTC 
CTTATCGCAA TGCTTTTTTT CATTTACGCC ATCATCGGGA TGCAGGTGTT TGGTAACATC 
GCGTTGAACA ATGAAGATTC GGCCATCAAC ATTCACAATA ACTTCCGCAC CTTTTTTCAG 
GCCCTCATGT TGTTGTTCAG AAGTGCTACA GGGGAGGCTT GGCACGAGAT CATGTTGGCT 
TGTCTGAGTG AAAGATATTG CGATATAGAA TCTGGCTACA GTGGGAAAGA GTGCGGCAGC 
GATTTTGCAT ACTTCTACTT CGTCTCCTTC ATCTTTCTTT GCTCGTTTCT GATGCTGAAT 
CTGTTTGTGG CTGTCATCAT GGATAATTTT GAATATCTGA CACGAGATGC TTCTATTCTG 
GGGCCTCATC ACCTAGATGA GTTCATCCGC GTCTGGGCTG AGTACGACCC TGCCGCCTGT 
GGACGCATGT CCTACCTCGA TATGTACGAG ATGCTCCGCC ACATGTCCCC TCCGCTAGGT 
TTGGGAAAGA AATGTCCTCC ACGAATCGCC TACAAGCGAT TGGTGAGGAT GAACATGCCC 
ATAGCCGAGG ACAACACGGT TCACTTTACC TCCACTTTAA TGGCTCTCAT CCGGACTGCC 
CTGGAGATCA AACTGGCATC AGGAGTTCTG GCTCAGCGCA TTTGTGATGC TGAATTAAGG 
AAGGAAATCA ATAAAATTTG GCCCAGTTTG TCATCTAAAA CAGTAGACCT GCTGGTTACA 
CCTCATAAAC ATAACGAATT GACTGTCGGG AAGGTCTATG CAGCGCTCAT GATTTTTGAC 
TACTATAAGC AGAACAGAGC CAAAAGACTC CAACAGCAGC AAGCTGCACC AGGCACACAG 
AGTAAAGTCG GTGTTCTCTT CAGGCCGATG CTTCCTCTCA CACACCTGCA TGACCAGGAG 
CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT CATTTAGATA TCACCCCTCA GTCAGATGCC 
ACACCCACTT CTTTTCACTT GACCAATGGG GATGCAGTTC AAAGCCAGAG CAGTGCCATG 
AAAAAGTCTC CTTCGGGAGG CCCATCTCAG GAAGTCACAC AGGAAACGAA CAGAAGGCCT 
ATCCAACGTG GTCAATCGGA AGACGTGCCA AACACTTACA GATCTCAGGA ACTAGTAGAG 
ATGACTGACA TGGAGAATGA TTCGGATGTT GGCGGGTATC ATGTGCTGGA GGGTCATGGC 
AGAGCTGCCT CTATGCCCAG ACTGAATGCT GGCTTCCAGC GGAGCCACTT ACGCCACACC 
GCCGGAGCGT ATCTAGCACC AATTGCAGAT GTGAGTCCCA TGCGGCGATC CACATCCTCG 
CTGACTCCAC AAAGAGGTGC GCGAGAGGTC AACCTGAGCG AATACGACCT GGAGAGACCC 
AGGATGAGCC CGAGCCAGAC TTTCGCTCAG GACAGAGAGA GAGAGAGAGT CCACCATCAT 
CATCACCACC ATCATCATCG CTGTCAGAGA CGCAGAGACA AGAAGCACAA GTCACTGGAT 
CGAGCCATCA GCGAGGAGCA GCCGCCACCT GCAGCTGATC CAAACACAGA GTCGACGCCC 
AGGGAACGAG CCCGGGAGCG AGACCGTGGC CGCTCACACG AGAGGAAGCA CCACTCATCC 
TCAGCAGCAG AAAGGAAGCA GCGATACTAC TCTTGTGACC GCTACGGCAG CCATGAACAT 
GGCCACAGCC TGTCCGCCGG GCCTAGCCGA TCCACCTCTC CTGGAGAACC ACATGACAGG 
CTTAAACAGG GAATTAACGC AGCTAAAGGA AGCTCAGTGG GTCATACCTC AGGCACCAGC 
ACACCCTGCC GCGGGCGTAG ACAGCTTCCC CAGACACCTC TTACCCCTCG GCCTGCTGTT 
GCCTATAAGA CTGCCAACTC TTCTCCGGTC CAGCTCAACA CTGCCCAATC TACAGGGCCC 
TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA GAGCACGATA CCTTACTCAG CGGTAGCATC 
CACCGCCAAT CCCCTGTGCC TGTCACTCGT ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC 
CAGCAAGACT CGCACTTGTC TGAAGCGGAA GACTTTCACG ATGCCTTGAG CACGTACGGC 
ACCGGTCGCT CCCCTAGAAC TACCGCCATC ATCGCCCACA CGTCATCTGG TGCGACCGCC 
ACTTCTACGC TGCTGTCCCG AAGCCAGAGC GGAGTCCCCA ACGGGTACCA CTACTCCCTC 
GGAGTGAACG CGGCTCCGGG ATTGAGTGGG AGAGCGTCTG GAAGCTACCA GGAGACTGAG 
AGAGATGACT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_017352992.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208481.2 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

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CloneID	ODa151031
Clone ID Related Accession (Same CDS sequence)	XM_017352992.2
Accession Version	XM_017352992.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC 
ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC 
TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC 
ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT 
CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG 
GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT 
GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC 
TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA 
TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC 
TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG 
CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC 
AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC 
ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT 
CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA 
TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG 
GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA 
CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT 
CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT 
TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT 
GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA 
GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC 
ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC 
GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC 
TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG 
AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC 
CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC 
AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC 
AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA 
GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC 
TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT 
TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC 
ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC 
TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG 
ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG 
CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG 
CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT 
GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC 
TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC 
GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA 
GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA 
GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208481.2
CDS	556..7560
Translation	MSRMARFGDDLPTRYGGPAGAGRGGARAGGPSGGQRMYKQSMAQRARTMALYNPKQTKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYLILTTIIANCIVLALEQHLPALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKTDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352992.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352992.2

ORF Insert Sequence:

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGACGG ATAAGAGGAT TAATCTGAGT TTCCTGAGAC TCTTCCGGGC GGCTCGTCTC 
ATTAAACTCC TTCGGCAAGG ATACACCATT CGGATCCTTC TCTGGACCTT TGTTCAGTCC 
TTTAAGGCTC TTCCATATGT GTGTCTCCTT ATCGCAATGC TTTTTTTCAT TTACGCCATC 
ATCGGGATGC AGGTGTTTGG TAACATCGCG TTGAACAATG AAGATTCGGC CATCAACATT 
CACAATAACT TCCGCACCTT TTTTCAGGCC CTCATGTTGT TGTTCAGAAG TGCTACAGGG 
GAGGCTTGGC ACGAGATCAT GTTGGCTTGT CTGAGTGAAA GATATTGCGA TATAGAATCT 
GGCTACAGTG GGAAAGAGTG CGGCAGCGAT TTTGCATACT TCTACTTCGT CTCCTTCATC 
TTTCTTTGCT CGTTTCTGAT GCTGAATCTG TTTGTGGCTG TCATCATGGA TAATTTTGAA 
TATCTGACAC GAGATGCTTC TATTCTGGGG CCTCATCACC TAGATGAGTT CATCCGCGTC 
TGGGCTGAGT ACGACCCTGC CGCCTGTGGA CGCATGTCCT ACCTCGATAT GTACGAGATG 
CTCCGCCACA TGTCCCCTCC GCTAGGTTTG GGAAAGAAAT GTCCTCCACG AATCGCCTAC 
AAGCGATTGG TGAGGATGAA CATGCCCATA GCCGAGGACA ACACGGTTCA CTTTACCTCC 
ACTTTAATGG CTCTCATCCG GACTGCCCTG GAGATCAAAC TGGCATCAGG AGTTCTGGCT 
CAGCGCATTT GTGATGCTGA ATTAAGGAAG GAAATCAATA AAATTTGGCC CAGTTTGTCA 
TCTAAAACAG TAGACCTGCT GGTTACACCT CATAAACATA ACGAATTGAC TGTCGGGAAG 
GTCTATGCAG CGCTCATGAT TTTTGACTAC TATAAGCAGA ACAGAGCCAA AAGACTCCAA 
CAGCAGCAAG CTGCACCAGG CACACAGAGT AAAGTCGGTG TTCTCTTCAG GCCGATGCTT 
CCTCTCACAC ACCTGCATGA CCAGGAGCCT TTAGTGCCAA AAGGCCCGCC TCCTTCTCAT 
TTAGATATCA CCCCTCAGTC AGATGCCACA CCCACTTCTT TTCACTTGAC CAATGGGGAT 
GCAGTTCAAA GCCAGAGCAG TGCCATGAAA AAGTCTCCTT CGGGAGGCCC ATCTCAGGAA 
GTCACACAGG AAACGAACAG AAGGCCTATC CAACGTGGTC AATCGGAAGA CGTGCCAAAC 
ACTTACAGAT CTCAGGAACT AGTAGAGATG ACTGACATGG AGAATGATTC GGATGTTGGC 
GGGTATCATG TGCTGGAGGG TCATGGCAGA GCTGCCTCTA TGCCCAGACT GAATGCTGGC 
TTCCAGCGGA GCCACTTACG CCACACCGCC GGAGCGTATC TAGCACCAAT TGCAGATGTG 
AGTCCCATGC GGCGATCCAC ATCCTCGCTG ACTCCACAAA GAGGTGCGCG AGAGGTCAAC 
CTGAGCGAAT ACGACCTGGA GAGACCCAGG ATGAGCCCGA GCCAGACTTT CGCTCAGGAC 
AGAGAGAGAG AGAGAGTCCA CCATCATCAT CACCACCATC ATCATCGCTG TCAGAGACGC 
AGAGACAAGA AGCACAAGTC ACTGGATCGA GCCATCAGCG AGGAGCAGCC GCCACCTGCA 
GCTGATCCAA ACACAGAGTC GACGCCCAGG GAACGAGCCC GGGAGCGAGA CCGTGGCCGC 
TCACACGAGA GGAAGCACCA CTCATCCTCA GCAGCAGAAA GGAAGCAGCG ATACTACTCT 
TGTGACCGCT ACGGCAGCCA TGAACATGGC CACAGCCTGT CCGCCGGGCC TAGCCGATCC 
ACCTCTCCTG GAGAACCACA TGACAGGCTT AAACAGGGAA TTAACGCAGC TAAAGGAAGC 
TCAGTGGGTC ATACCTCAGG CACCAGCACA CCCTGCCGCG GGCGTAGACA GCTTCCCCAG 
ACACCTCTTA CCCCTCGGCC TGCTGTTGCC TATAAGACTG CCAACTCTTC TCCGGTCCAG 
CTCAACACTG CCCAATCTAC AGGGCCCTGC CCCGCTCGCC TTAGTCGTGG CCTGTCAGAG 
CACGATACCT TACTCAGCGG TAGCATCCAC CGCCAATCCC CTGTGCCTGT CACTCGTATT 
GGCTCTGACC CCAGCTTAGG TCCCCTCCAG CAAGACTCGC ACTTGTCTGA AGCGGAAGAC 
TTTCACGATG CCTTGAGCAC GTACGGCACC GGTCGCTCCC CTAGAACTAC CGCCATCATC 
GCCCACACGT CATCTGGTGC GACCGCCACT TCTACGCTGC TGTCCCGAAG CCAGAGCGGA 
GTCCCCAACG GGTACCACTA CTCCCTCGGA GTGAACGCGG CTCCGGGATT GAGTGGGAGA 
GCGTCTGGAA GCTACCAGGA GACTGAGAGA GATGACTGGT GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_017352993.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208482.2 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

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CloneID	ODa151032
Clone ID Related Accession (Same CDS sequence)	XM_017352993.2
Accession Version	XM_017352993.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352993.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT 
AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT 
AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC 
GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC 
AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG 
GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC 
TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT 
CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT 
CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG 
GCTGAGTACG ACCCTGCCGC CTGTGGACGC ATGTCCTACC TCGATATGTA CGAGATGCTC 
CGCCACATGT CCCCTCCGCT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT 
TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG 
CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT 
AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC 
TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG 
CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT 
CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA 
GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA 
GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC 
ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT 
TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG 
TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC 
CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT 
CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG 
AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA 
GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA 
GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT 
GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA 
CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT 
GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC 
TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA 
GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA 
CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC 
AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC 
GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC 
TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT 
CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC 
CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC 
CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG 
TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208482.2
CDS	556..7557
Translation	MSRMARFGDDLPTRYGGPAGAGRGGARAGGPSGGQRMYKQSMAQRARTMALYNPKQTKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYLILTTIIANCIVLALEQHLPALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352993.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352993.2

ORF Insert Sequence:

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT 
AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT 
AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC 
GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC 
AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG 
GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC 
TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT 
CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT 
CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG 
GCTGAGTACG ACCCTGCCGC CTGTGGACGC ATGTCCTACC TCGATATGTA CGAGATGCTC 
CGCCACATGT CCCCTCCGCT AGGTTTGGGA AAGAAATGTC CTCCACGAAT CGCCTACAAG 
CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT 
TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG 
CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT 
AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC 
TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG 
CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT 
CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA 
GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA 
GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC 
ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT 
TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG 
TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC 
CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT 
CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG 
AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA 
GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA 
GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT 
GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA 
CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT 
GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC 
TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA 
GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA 
CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC 
AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC 
GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC 
TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT 
CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC 
CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC 
CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG 
TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_017352994.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208483.2 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

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CloneID	ODa151033
Clone ID Related Accession (Same CDS sequence)	XM_017352994.2
Accession Version	XM_017352994.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT 
AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT 
AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC 
GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC 
AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG 
GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC 
TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT 
CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT 
CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG 
GCTGAGTACG ACCCTGCCGC CTGTGGAAGG ATTCCCTATA TGGATATGTA CAATTTGTTG 
CGTGTAATCT CACCTCCCCT TGGCTTAGGA AAGAACTGCC CTAATAGGGT GGCATACAAG 
CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT 
TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG 
CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT 
AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC 
TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG 
CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT 
CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA 
GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA 
GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC 
ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT 
TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG 
TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC 
CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT 
CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG 
AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA 
GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA 
GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT 
GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA 
CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT 
GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC 
TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA 
GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA 
CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC 
AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC 
GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC 
TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT 
CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC 
CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC 
CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG 
TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208483.2
CDS	556..7557
Translation	MSRMARFGDDLPTRYGGPAGAGRGGARAGGPSGGQRMYKQSMAQRARTMALYNPKQTKQNCFTVNRSLFIFSEDNVIRKYAKRITEWPPFEYLILTTIIANCIVLALEQHLPALDKTPMSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRIPYMDMYNLLRVISPPLGLGKNCPNRVAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352994.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352994.2

ORF Insert Sequence:

ATGTCTAGGA TGGCTCGCTT TGGAGACGAT CTGCCCACCC GCTATGGAGG TCCAGCGGGC 
GCCGGGAGAG GGGGAGCCCG GGCGGGAGGT CCCTCGGGCG GTCAAAGAAT GTACAAACAG 
TCGATGGCTC AGAGAGCCAG GACAATGGCT TTATATAATC CTAAACAAAC CAAACAGAAC 
TGTTTCACTG TCAACCGCTC TTTGTTCATC TTCAGCGAGG ACAATGTCAT CAGGAAATAT 
GCCAAAAGAA TAACAGAATG GCCTCCATTT GAGTACCTGA TCCTCACCAC CATCATCGCC 
AACTGCATTG TCCTGGCCCT CGAACAGCAT CTCCCAGCCT TGGACAAGAC CCCCATGTCT 
GAACGCCTGG ACGACACAGA GCCCTACTTT ATCGGAATAT TCTGTTTCGA GGCCGGCATT 
AAGATCATCG CCTTAGGCTT CGTGTTTCAC AAAGACTCTT ACCTTCGCAA CGGCTGGAAC 
ATAATGGACT TTGTGGTCGT TGTGACTGGG ATTTTGACCA CAGTGGGTTC ACAGTTCGAC 
TTGAGGACGT TGAGGGCCGT TAGAGTGCTG AGACCCCTCA AACTGGTGTC AGGAATACCA 
AGTCTTCAAG TAGTATTAAA GTCCATCATG AAGGCCATGG TTCCCCTTCT TCAGATCGGA 
TTGCTGCTCT TCTTTGCCAT CGTTATGTTT GCCATTATTG GTGTCGAGTT TTACATGGGC 
AAGTTCCACT TCACCTGCTT CAAAGTGGAT ACAGGAGAAA AGGCTGTTGA TTATCCATGT 
GGAGCTGAGC CTCCCTCAAG ATCGTGTCCC GTTGGAACAA ACTGTACGGA GTACTGGAAA 
GGACCCAATT TTGGCATCAC AAATTTCGAT AACATCCTCT TCGCCATCCT CACAGTCTTC 
CAGTGCATCA CCATGGAAGG ATGGACTGAT GTACTATACC ATACCAATGA TGCCGCGGGA 
AACACCTGGA ATTGGCTGTA CTTTATCCCT CTCATCATTA TCGGCTCTTT TTTCATGCTT 
AACCTCGTCC TGGGTGTCTT ATCAGGAGAG TTTGCCAAAG AGAGGGAGCG AGTGGAGAAG 
AGGCAGGAGT TCTTAAAGCT TCGCAGACAG CAGCAGATTG AGAGGGAGCT CACCGGCTAC 
TTGGAATGGA TCTGCAAAGC AGAGGAGGTG CTGCTGGCGG AGGAAGACCA GAACGCTGAG 
GAGAAATCCC CTCTGGATGG TGCGTGGTAC AGGCGGAAAC AGAACAACCC AGTTCTAAAA 
AGAACAAAGT CCAGAAAGGG TAGAAATGAC CTCATCAGTG CTGAAGAGGG AGACGAACAT 
TTCACAGACA TCTCCTCTGT CGCACCACCT GGATCTCCTT TTGCCAGAGC TAGCCTGAAG 
AGCAGTAAGA ACGACAGCTC CTCATATTTC CGTCGAAAGG AAAAAAGGAT TCGCTTTTTC 
ATCCGGCGGA TGGTGAAGGC ACAGAGTTTC TACTGGATCG TTTTGTGTTT GGTGGGATTG 
AACACGATGT GTGTGGCTAT TGTCCATTAT GACCAGCCGG AGTGGCTCAC CAAGGCCCTG 
TACCTGGCAG AGTTTGTATT CCTGGGTCTG TTTCTGACAG AGATGACTCT GAAGATGTAC 
GGCCTGGGGC CCAGAAACTA CTTTCACTCC TCGTTCAACT GTTTTGACTT TGGGGTAATT 
GTGGGGAGTA TATTTGAGGT GATCTGGGCA GCGATTCAGC CTGGTGCGTC TTTTGGAATC 
AGTGTTTTGA GAGCCCTGCG TCTGCTCCGT ATCTTCAAAG TAACCAAATA CTGGAACTCT 
TTGCGGAATC TCGTGGTTTC TCTGTTAAAC TCCATGAAGT CCATCATCAG CTTGCTCTTC 
CTCCTCTTCC TCTTCATCGT GGTGTTTGCT CTGCTTGGCA TGCAGCTCTT CGGGGGCCAG 
TTTAATTTTG AAGATGAAAC ACCCACTACA AATTTTGACA GCTTCCCTGC AGCCATAATG 
ACAGTCTTCC AGATTTTGAC AGGCGAGGAT TGGAACGCAG TGATGTATCA TGGCATCGAG 
TCGCAGGGTG GCGTCCGGGG TGGGATGTTC TCCTCGGTTT ACTTCATTGT TCTTACTCTC 
TTCGGAAACT ACACACTTCT CAATGTATTC TTGGCCATCG CTGTGGATAA CCTTGCCAAT 
GCGCAGGAGC TGACAAAGGA TGAAGAGCAG CAGGAGGAGG CAGCGAAGAA AAGTCTGGCT 
CTTCAGAAGG CCATGGAGGT GAAAGAGGTC AGCCCCATGT CTGCTGCCAA CATCTCCATC 
GCAGCTTTTG TAAAGCAAAA TCGAGATGCA ATCTCTCGCA GGTCTTCAGT CTGCAGCGTT 
CATTCACCTA AAGAGCAGCA GAGGTCACTG CAGAAGTTGT CCATATGGGA GCAGCGCACT 
AACCAACTGC GCAGACACAA CCTGCGTAGC AGCAGCGAGG CACTTTACAA CGAATTGGAG 
CCAGAAGAGC GTCTGCGTGT CTCCTCTGCC CTGCATCTGC GGCCTGACAT GAAGACCCAC 
CACGATCGGC CGCTGGTTGT GGAGCACCGA GATTCTGTGG ACAATCTCAG ATCTGACAAA 
GACGGAGACA CTTCAGAGGG TCAGCCAGCA TCTAACCACC AGAGAAAATA TCATAAATGT 
CGAGGGGAGA ACGGAGACAC CGGCGAGGGA CGGCTTCACA GACACCACAG CCAAAATCGA 
GAGAACCAGG GTGGCCTAGA GCGCAGCAAC AGTCAAGATG GAGGACAGAG GAACCACCAC 
AGATCTTCTG GATCTCCAGA GGAGGGCATT GGGATTGAGG AGAGAGAACA TAGACATCAT 
CATTCTAGAA GACCCAGAGA AGTCAACGGG AACGGGAATG GTGTGATTGG TAATGGAGGC 
AGGGGCGAGA GGATACGAGG TCACAGAGAG GGTGAAGGAG GGAATGGAGA GAGAAGGAGA 
CACCGGCCAC GGGTTAAAGC CCAGTCCACA TTGGATGGAG ACGAATGTAG AGAGAATGGT 
GGTAAACGCA GGAGCAGACC AACAGAAACA GACAGCCTCA CAACCAGCCC TCTCCAGTCT 
CCTTCTGAAG AGAAGCCAGA AGACAAAGAT AATCTAAAAA ACAGCACTCG AGTGGGTCCA 
AGCGGAGTCA ACATCCCTGT CACCATAACT GCGCCACCAG GAGAGACCAC TGTCATTCCA 
ATGAACAACA TTGATGGTGA CAGTCTGCTG CTGAATGAGG AAAAGAAGGA TCTTGATGAG 
CTAAATCAGA ATGCGCCTAA ACACATTTTG CCATACAGCT CCATGTTTGT GTTTGGCCCA 
ACCAACCCAG TGCGACGACT GTGTCATTAT GTGGTGAATC TGCGGTATTT TGAGATGTGC 
ATCCTCACAG TCATCACCAT GAGCAGTATC GCTCTCGCTG CCGAAGACCC CGTGCAGGCT 
CATGCCCCTC GCAATGATGT GCTGAAATAC CTTGATTACG TCTTCACTGG TGTGTTCACA 
TTTGAGATGG TTATAAAGAT GATTGATCTT GGACTGCTGC TGCATCCCGG CTCTTACTTC 
AGGGACCTGT GGAACATCCT GGACTTCATC GTAGTGAGCG GCGCTCTGGT GGCTTTCGCC 
TGCTCAGGAA CTAAAGGAAA GGACATAAAC ACCATCAAGT CCCTGAGGGT TCTTCGAGTC 
TTGAGACCCT TGAAGACAAT CAAAAGATTG CCCAAACTCA AGGCCGTGTT TGACTGCGTG 
GTGAACTCTC TGAAGAATGT GCTCAACATC CTCATCGTCT ACATCCTCTT CATGTTCATC 
TTCGCGGTCA TCGCCGTGCA GCTTTTCAAG GGGAAGTTTT TCCACTGCAC CGATGAGTCC 
AAAGCCCTGG AGAAGGACTG CAGGGGTCAG TTCTTGGAGT ACGGCAGTGA TGGAGTGGCC 
ATGACGCAGC CGCGCGAGTG GAAGAAGTAC GACTTCCATT ATGACAACGT CCTGTGGGCC 
TTTCTGACCC TCTTCACTGT GTCTACAGGA GAGGGCTGGC CACTTGTGCT GAAACACTCT 
GTTGATGCCA CGTATGAAGA CCAGGGTCCC AGTCCAGGAT ATCGCATGGA AACCTCCATC 
TTCTATGTGG TCTACTTCAT CGTCTTTCCC TTCTTCTTTG TCAACATCTT TGTGGCTTTG 
ATCATCATCA CATTCCAGGA GCAGGGGGAT AAAGCCATGT CCGAGTGCAG CTTGGAAAAG 
AACGAGCGAG CTTGTATTGA CTTTGCCATC AATGCTAAAC CTCTGACTCG CTACATGCCG 
CAAGACAAGA ACTCTTACCA GTACAAGATG TGGAAGTTTG TGGTGTCGAC TCCGTTTGAG 
TACTCCATCC TGACCATGAT CGCCATCAAT ACAGTGGTGC TCATGATGAA GTTCCATGGT 
GCTCCTAAAC CTTATGAGGA CATGTTGAAG TGGCTGAACA TCATCTTCAC TGCACTCTTC 
ACACTGGAGT GTGTTCTCAA GGTCATCGCC TTCGGTCCTC TGAACTACCT GAAAGAGGCT 
TGGAACATTT TTGACTTTGT TACTGTTCTG GGAAGCATCA CAGACATCCT GGTTACTGAG 
ATCAAGGATA AGAGGATTAA TCTGAGTTTC CTGAGACTCT TCCGGGCGGC TCGTCTCATT 
AAACTCCTTC GGCAAGGATA CACCATTCGG ATCCTTCTCT GGACCTTTGT TCAGTCCTTT 
AAGGCTCTTC CATATGTGTG TCTCCTTATC GCAATGCTTT TTTTCATTTA CGCCATCATC 
GGGATGCAGG TGTTTGGTAA CATCGCGTTG AACAATGAAG ATTCGGCCAT CAACATTCAC 
AATAACTTCC GCACCTTTTT TCAGGCCCTC ATGTTGTTGT TCAGAAGTGC TACAGGGGAG 
GCTTGGCACG AGATCATGTT GGCTTGTCTG AGTGAAAGAT ATTGCGATAT AGAATCTGGC 
TACAGTGGGA AAGAGTGCGG CAGCGATTTT GCATACTTCT ACTTCGTCTC CTTCATCTTT 
CTTTGCTCGT TTCTGATGCT GAATCTGTTT GTGGCTGTCA TCATGGATAA TTTTGAATAT 
CTGACACGAG ATGCTTCTAT TCTGGGGCCT CATCACCTAG ATGAGTTCAT CCGCGTCTGG 
GCTGAGTACG ACCCTGCCGC CTGTGGAAGG ATTCCCTATA TGGATATGTA CAATTTGTTG 
CGTGTAATCT CACCTCCCCT TGGCTTAGGA AAGAACTGCC CTAATAGGGT GGCATACAAG 
CGATTGGTGA GGATGAACAT GCCCATAGCC GAGGACAACA CGGTTCACTT TACCTCCACT 
TTAATGGCTC TCATCCGGAC TGCCCTGGAG ATCAAACTGG CATCAGGAGT TCTGGCTCAG 
CGCATTTGTG ATGCTGAATT AAGGAAGGAA ATCAATAAAA TTTGGCCCAG TTTGTCATCT 
AAAACAGTAG ACCTGCTGGT TACACCTCAT AAACATAACG AATTGACTGT CGGGAAGGTC 
TATGCAGCGC TCATGATTTT TGACTACTAT AAGCAGAACA GAGCCAAAAG ACTCCAACAG 
CAGCAAGCTG CACCAGGCAC ACAGAGTAAA GTCGGTGTTC TCTTCAGGCC GATGCTTCCT 
CTCACACACC TGCATGACCA GGAGCCTTTA GTGCCAAAAG GCCCGCCTCC TTCTCATTTA 
GATATCACCC CTCAGTCAGA TGCCACACCC ACTTCTTTTC ACTTGACCAA TGGGGATGCA 
GTTCAAAGCC AGAGCAGTGC CATGAAAAAG TCTCCTTCGG GAGGCCCATC TCAGGAAGTC 
ACACAGGAAA CGAACAGAAG GCCTATCCAA CGTGGTCAAT CGGAAGACGT GCCAAACACT 
TACAGATCTC AGGAACTAGT AGAGATGACT GACATGGAGA ATGATTCGGA TGTTGGCGGG 
TATCATGTGC TGGAGGGTCA TGGCAGAGCT GCCTCTATGC CCAGACTGAA TGCTGGCTTC 
CAGCGGAGCC ACTTACGCCA CACCGCCGGA GCGTATCTAG CACCAATTGC AGATGTGAGT 
CCCATGCGGC GATCCACATC CTCGCTGACT CCACAAAGAG GTGCGCGAGA GGTCAACCTG 
AGCGAATACG ACCTGGAGAG ACCCAGGATG AGCCCGAGCC AGACTTTCGC TCAGGACAGA 
GAGAGAGAGA GAGTCCACCA TCATCATCAC CACCATCATC ATCGCTGTCA GAGACGCAGA 
GACAAGAAGC ACAAGTCACT GGATCGAGCC ATCAGCGAGG AGCAGCCGCC ACCTGCAGCT 
GATCCAAACA CAGAGTCGAC GCCCAGGGAA CGAGCCCGGG AGCGAGACCG TGGCCGCTCA 
CACGAGAGGA AGCACCACTC ATCCTCAGCA GCAGAAAGGA AGCAGCGATA CTACTCTTGT 
GACCGCTACG GCAGCCATGA ACATGGCCAC AGCCTGTCCG CCGGGCCTAG CCGATCCACC 
TCTCCTGGAG AACCACATGA CAGGCTTAAA CAGGGAATTA ACGCAGCTAA AGGAAGCTCA 
GTGGGTCATA CCTCAGGCAC CAGCACACCC TGCCGCGGGC GTAGACAGCT TCCCCAGACA 
CCTCTTACCC CTCGGCCTGC TGTTGCCTAT AAGACTGCCA ACTCTTCTCC GGTCCAGCTC 
AACACTGCCC AATCTACAGG GCCCTGCCCC GCTCGCCTTA GTCGTGGCCT GTCAGAGCAC 
GATACCTTAC TCAGCGGTAG CATCCACCGC CAATCCCCTG TGCCTGTCAC TCGTATTGGC 
TCTGACCCCA GCTTAGGTCC CCTCCAGCAA GACTCGCACT TGTCTGAAGC GGAAGACTTT 
CACGATGCCT TGAGCACGTA CGGCACCGGT CGCTCCCCTA GAACTACCGC CATCATCGCC 
CACACGTCAT CTGGTGCGAC CGCCACTTCT ACGCTGCTGT CCCGAAGCCA GAGCGGAGTC 
CCCAACGGGT ACCACTACTC CCTCGGAGTG AACGCGGCTC CGGGATTGAG TGGGAGAGCG 
TCTGGAAGCT ACCAGGAGAC TGAGAGAGAT GACTGGTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_017352991.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208480.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

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CloneID	ODa56940
Clone ID Related Accession (Same CDS sequence)	XM_017352991.1
Accession Version	XM_017352991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC 
TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG 
TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA 
ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG 
ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC 
ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT 
CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC 
TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA 
GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC 
GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC 
ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG 
GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC 
GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC 
GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT 
CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC 
GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC 
CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC 
TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG 
GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG 
GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG 
ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG 
GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT 
GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG 
AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG 
CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG 
GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC 
ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC 
ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT 
ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT 
GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT 
CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC 
TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC 
AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG 
CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA 
TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG 
ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT 
GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT 
AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA 
AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC 
CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA 
CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT 
GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA 
AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG 
AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC 
CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG 
GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC 
ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT 
GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT 
GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG 
ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG 
CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG 
TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT 
TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT 
TTCGCCTGCT CGAGCTTCAT GGGAGGAACT AAAGGAAAGG ACATAAACAC CATCAAGTCC 
CTGAGGGTTC TTCGAGTCTT GAGACCCTTG AAGACAATCA AAAGATTGCC CAAACTCAAG 
GCCGTGTTTG ACTGCGTGGT GAACTCTCTG AAGAATGTGC TCAACATCCT CATCGTCTAC 
ATCCTCTTCA TGTTCATCTT CGCGGTCATC GCCGTGCAGC TTTTCAAGGG GAAGTTTTTC 
CACTGCACCG ATGAGTCCAA AGCCCTGGAG AAGGACTGCA GGGGTCAGTT CTTGGAGTAC 
GGCAGTGATG GAGTGGCCAT GACGCAGCCG CGCGAGTGGA AGAAGTACGA CTTCCATTAT 
GACAACGTCC TGTGGGCCTT TCTGACCCTC TTCACTGTGT CTACAGGAGA GGGCTGGCCA 
CTTGTGCTGA AACACTCTGT TGATGCCACG TATGAAGACC AGGGTCCCAG TCCAGGATAT 
CGCATGGAAA CCTCCATCTT CTATGTGGTC TACTTCATCG TCTTTCCCTT CTTCTTTGTC 
AACATCTTTG TGGCTTTGAT CATCATCACA TTCCAGGAGC AGGGGGATAA AGCCATGTCC 
GAGTGCAGCT TGGAAAAGAA CGAGCGAGCT TGTATTGACT TTGCCATCAA TGCTAAACCT 
CTGACTCGCT ACATGCCGCA AGACAAGAAC TCTTACCAGT ACAAGATGTG GAAGTTTGTG 
GTGTCGACTC CGTTTGAGTA CTCCATCCTG ACCATGATCG CCATCAATAC AGTGGTGCTC 
ATGATGAAGT TCCATGGTGC TCCTAAACCT TATGAGGACA TGTTGAAGTG GCTGAACATC 
ATCTTCACTG CACTCTTCAC ACTGGAGTGT GTTCTCAAGG TCATCGCCTT CGGTCCTCTG 
AACTACCTGA AAGAGGCTTG GAACATTTTT GACTTTGTTA CTGTTCTGGG AAGCATCACA 
GACATCCTGG TTACTGAGAT CAAGACGGAT AAGAGGATTA ATCTGAGTTT CCTGAGACTC 
TTCCGGGCGG CTCGTCTCAT TAAACTCCTT CGGCAAGGAT ACACCATTCG GATCCTTCTC 
TGGACCTTTG TTCAGTCCTT TAAGGCTCTT CCATATGTGT GTCTCCTTAT CGCAATGCTT 
TTTTTCATTT ACGCCATCAT CGGGATGCAG GTGTTTGGTA ACATCGCGTT GAACAATGAA 
GATTCGGCCA TCAACATTCA CAATAACTTC CGCACCTTTT TTCAGGCCCT CATGTTGTTG 
TTCAGAAGTG CTACAGGGGA GGCTTGGCAC GAGATCATGT TGGCTTGTCT GAGTGAAAGA 
TATTGCGATA TAGAATCTGG CTACAGTGGG AAAGAGTGCG GCAGCGATTT TGCATACTTC 
TACTTCGTCT CCTTCATCTT TCTTTGCTCG TTTCTGATGC TGAATCTGTT TGTGGCTGTC 
ATCATGGATA ATTTTGAATA TCTGACACGA GATGCTTCTA TTCTGGGGCC TCATCACCTA 
GATGAGTTCA TCCGCGTCTG GGCTGAGTAC GACCCTGCCG CCTGTGGAAG GATTCCCTAT 
ATGGATATGT ACAATTTGTT GCGTGTAATC TCACCTCCCC TTGGCTTAGG AAAGAACTGC 
CCTAATAGGG TGGCATACAA GCGATTGGTG AGGATGAACA TGCCCATAGC CGAGGACAAC 
ACGGTTCACT TTACCTCCAC TTTAATGGCT CTCATCCGGA CTGCCCTGGA GATCAAACTG 
GCATCAGGAG TTCTGGCTCA GCGCATTTGT GATGCTGAAT TAAGGAAGGA AATCAATAAA 
ATTTGGCCCA GTTTGTCATC TAAAACAGTA GACCTGCTGG TTACACCTCA TAAACATAAC 
GAATTGACTG TCGGGAAGGT CTATGCAGCG CTCATGATTT TTGACTACTA TAAGCAGAAC 
AGAGCCAAAA GACTCCAACA GCAGCAAGCT GCACCAGGCA CACAGAGTAA AGTCGGTGTT 
CTCTTCAGGC CGATGCTTCC TCTCACACAC CTGCATGACC AGGAGCCTTT AGTGCCAAAA 
GGCCCGCCTC CTTCTCATTT AGATATCACC CCTCAGTCAG ATGCCACACC CACTTCTTTT 
CACTTGACCA ATGGGGATGC AGTTCAAAGC CAGAGCAGTG CCATGAAAAA GTCTCCTTCG 
GGAGGCCCAT CTCAGGAAGT CACACAGGAA ACGAACAGAA GGCCTATCCA ACGTGGTCAA 
TCGGAAGACG TGCCAAACAC TTACAGATCT CAGGAACTAG TAGAGATGAC TGACATGGAG 
AATGATTCGG ATGTTGGCGG GTATCATGTG CTGGAGGGTC ATGGCAGAGC TGCCTCTATG 
CCCAGACTGA ATGCTGGCTT CCAGCGGAGC CACTTACGCC ACACCGCCGG AGCGTATCTA 
GCACCAATTG CAGATGTGAG TCCCATGCGG CGATCCACAT CCTCGCTGAC TCCACAAAGA 
GGTGCGCGAG AGGTCAACCT GAGCGAATAC GACCTGGAGA GACCCAGGAT GAGCCCGAGC 
CAGACTTTCG CTCAGGACAG AGAGAGAGAG AGAGTCCACC ATCATCATCA CCACCATCAT 
CATCGCTGTC AGAGACGCAG AGACAAGAAG CACAAGTCAC TGGATCGAGC CATCAGCGAG 
GAGCAGCCGC CACCTGCAGC TGATCCAAAC ACAGAGTCGA CGCCCAGGGA ACGAGCCCGG 
GAGCGAGACC GTGGCCGCTC ACACGAGAGG AAGCACCACT CATCCTCAGC AGCAGAAAGG 
AAGCAGCGAT ACTACTCTTG TGACCGCTAC GGCAGCCATG AACATGGCCA CAGCCTGTCC 
GCCGGGCCTA GCCGATCCAC CTCTCCTGGA GAACCACATG ACAGGCTTAA ACAGGGAATT 
AACGCAGCTA AAGGAAGCTC AGTGGGTCAT ACCTCAGGCA CCAGCACACC CTGCCGCGGG 
CGTAGACAGC TTCCCCAGAC ACCTCTTACC CCTCGGCCTG CTGTTGCCTA TAAGACTGCC 
AACTCTTCTC CGGTCCAGCT CAACACTGCC CAATCTACAG GGCCCTGCCC CGCTCGCCTT 
AGTCGTGGCC TGTCAGAGCA CGATACCTTA CTCAGCGGTA GCATCCACCG CCAATCCCCT 
GTGCCTGTCA CTCGTATTGG CTCTGACCCC AGCTTAGGTC CCCTCCAGCA AGACTCGCAC 
TTGTCTGAAG CGGAAGACTT TCACGATGCC TTGAGCACGT ACGGCACCGG TCGCTCCCCT 
AGAACTACCG CCATCATCGC CCACACGTCA TCTGGTGCGA CCGCCACTTC TACGCTGCTG 
TCCCGAAGCC AGAGCGGAGT CCCCAACGGG TACCACTACT CCCTCGGAGT GAACGCGGCT 
CCGGGATTGA GTGGGAGAGC GTCTGGAAGC TACCAGGAGA CTGAGAGAGA TGACTGGTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208480.1
CDS	87..6749
Translation	MSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSSFMGGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKTDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRIPYMDMYNLLRVISPPLGLGKNCPNRVAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352991.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352991.1

ORF Insert Sequence:

ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC 
TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG 
TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA 
ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG 
ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC 
ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT 
CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC 
TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA 
GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC 
GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC 
ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG 
GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC 
GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC 
GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT 
CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC 
GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC 
CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC 
TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG 
GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG 
GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG 
ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG 
GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT 
GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG 
AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG 
CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG 
GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC 
ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC 
ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT 
ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT 
GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT 
CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC 
TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC 
AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG 
CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA 
TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG 
ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT 
GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT 
AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA 
AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC 
CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA 
CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT 
GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA 
AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG 
AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC 
CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG 
GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC 
ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT 
GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT 
GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG 
ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG 
CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG 
TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT 
TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT 
TTCGCCTGCT CGAGCTTCAT GGGAGGAACT AAAGGAAAGG ACATAAACAC CATCAAGTCC 
CTGAGGGTTC TTCGAGTCTT GAGACCCTTG AAGACAATCA AAAGATTGCC CAAACTCAAG 
GCCGTGTTTG ACTGCGTGGT GAACTCTCTG AAGAATGTGC TCAACATCCT CATCGTCTAC 
ATCCTCTTCA TGTTCATCTT CGCGGTCATC GCCGTGCAGC TTTTCAAGGG GAAGTTTTTC 
CACTGCACCG ATGAGTCCAA AGCCCTGGAG AAGGACTGCA GGGGTCAGTT CTTGGAGTAC 
GGCAGTGATG GAGTGGCCAT GACGCAGCCG CGCGAGTGGA AGAAGTACGA CTTCCATTAT 
GACAACGTCC TGTGGGCCTT TCTGACCCTC TTCACTGTGT CTACAGGAGA GGGCTGGCCA 
CTTGTGCTGA AACACTCTGT TGATGCCACG TATGAAGACC AGGGTCCCAG TCCAGGATAT 
CGCATGGAAA CCTCCATCTT CTATGTGGTC TACTTCATCG TCTTTCCCTT CTTCTTTGTC 
AACATCTTTG TGGCTTTGAT CATCATCACA TTCCAGGAGC AGGGGGATAA AGCCATGTCC 
GAGTGCAGCT TGGAAAAGAA CGAGCGAGCT TGTATTGACT TTGCCATCAA TGCTAAACCT 
CTGACTCGCT ACATGCCGCA AGACAAGAAC TCTTACCAGT ACAAGATGTG GAAGTTTGTG 
GTGTCGACTC CGTTTGAGTA CTCCATCCTG ACCATGATCG CCATCAATAC AGTGGTGCTC 
ATGATGAAGT TCCATGGTGC TCCTAAACCT TATGAGGACA TGTTGAAGTG GCTGAACATC 
ATCTTCACTG CACTCTTCAC ACTGGAGTGT GTTCTCAAGG TCATCGCCTT CGGTCCTCTG 
AACTACCTGA AAGAGGCTTG GAACATTTTT GACTTTGTTA CTGTTCTGGG AAGCATCACA 
GACATCCTGG TTACTGAGAT CAAGACGGAT AAGAGGATTA ATCTGAGTTT CCTGAGACTC 
TTCCGGGCGG CTCGTCTCAT TAAACTCCTT CGGCAAGGAT ACACCATTCG GATCCTTCTC 
TGGACCTTTG TTCAGTCCTT TAAGGCTCTT CCATATGTGT GTCTCCTTAT CGCAATGCTT 
TTTTTCATTT ACGCCATCAT CGGGATGCAG GTGTTTGGTA ACATCGCGTT GAACAATGAA 
GATTCGGCCA TCAACATTCA CAATAACTTC CGCACCTTTT TTCAGGCCCT CATGTTGTTG 
TTCAGAAGTG CTACAGGGGA GGCTTGGCAC GAGATCATGT TGGCTTGTCT GAGTGAAAGA 
TATTGCGATA TAGAATCTGG CTACAGTGGG AAAGAGTGCG GCAGCGATTT TGCATACTTC 
TACTTCGTCT CCTTCATCTT TCTTTGCTCG TTTCTGATGC TGAATCTGTT TGTGGCTGTC 
ATCATGGATA ATTTTGAATA TCTGACACGA GATGCTTCTA TTCTGGGGCC TCATCACCTA 
GATGAGTTCA TCCGCGTCTG GGCTGAGTAC GACCCTGCCG CCTGTGGAAG GATTCCCTAT 
ATGGATATGT ACAATTTGTT GCGTGTAATC TCACCTCCCC TTGGCTTAGG AAAGAACTGC 
CCTAATAGGG TGGCATACAA GCGATTGGTG AGGATGAACA TGCCCATAGC CGAGGACAAC 
ACGGTTCACT TTACCTCCAC TTTAATGGCT CTCATCCGGA CTGCCCTGGA GATCAAACTG 
GCATCAGGAG TTCTGGCTCA GCGCATTTGT GATGCTGAAT TAAGGAAGGA AATCAATAAA 
ATTTGGCCCA GTTTGTCATC TAAAACAGTA GACCTGCTGG TTACACCTCA TAAACATAAC 
GAATTGACTG TCGGGAAGGT CTATGCAGCG CTCATGATTT TTGACTACTA TAAGCAGAAC 
AGAGCCAAAA GACTCCAACA GCAGCAAGCT GCACCAGGCA CACAGAGTAA AGTCGGTGTT 
CTCTTCAGGC CGATGCTTCC TCTCACACAC CTGCATGACC AGGAGCCTTT AGTGCCAAAA 
GGCCCGCCTC CTTCTCATTT AGATATCACC CCTCAGTCAG ATGCCACACC CACTTCTTTT 
CACTTGACCA ATGGGGATGC AGTTCAAAGC CAGAGCAGTG CCATGAAAAA GTCTCCTTCG 
GGAGGCCCAT CTCAGGAAGT CACACAGGAA ACGAACAGAA GGCCTATCCA ACGTGGTCAA 
TCGGAAGACG TGCCAAACAC TTACAGATCT CAGGAACTAG TAGAGATGAC TGACATGGAG 
AATGATTCGG ATGTTGGCGG GTATCATGTG CTGGAGGGTC ATGGCAGAGC TGCCTCTATG 
CCCAGACTGA ATGCTGGCTT CCAGCGGAGC CACTTACGCC ACACCGCCGG AGCGTATCTA 
GCACCAATTG CAGATGTGAG TCCCATGCGG CGATCCACAT CCTCGCTGAC TCCACAAAGA 
GGTGCGCGAG AGGTCAACCT GAGCGAATAC GACCTGGAGA GACCCAGGAT GAGCCCGAGC 
CAGACTTTCG CTCAGGACAG AGAGAGAGAG AGAGTCCACC ATCATCATCA CCACCATCAT 
CATCGCTGTC AGAGACGCAG AGACAAGAAG CACAAGTCAC TGGATCGAGC CATCAGCGAG 
GAGCAGCCGC CACCTGCAGC TGATCCAAAC ACAGAGTCGA CGCCCAGGGA ACGAGCCCGG 
GAGCGAGACC GTGGCCGCTC ACACGAGAGG AAGCACCACT CATCCTCAGC AGCAGAAAGG 
AAGCAGCGAT ACTACTCTTG TGACCGCTAC GGCAGCCATG AACATGGCCA CAGCCTGTCC 
GCCGGGCCTA GCCGATCCAC CTCTCCTGGA GAACCACATG ACAGGCTTAA ACAGGGAATT 
AACGCAGCTA AAGGAAGCTC AGTGGGTCAT ACCTCAGGCA CCAGCACACC CTGCCGCGGG 
CGTAGACAGC TTCCCCAGAC ACCTCTTACC CCTCGGCCTG CTGTTGCCTA TAAGACTGCC 
AACTCTTCTC CGGTCCAGCT CAACACTGCC CAATCTACAG GGCCCTGCCC CGCTCGCCTT 
AGTCGTGGCC TGTCAGAGCA CGATACCTTA CTCAGCGGTA GCATCCACCG CCAATCCCCT 
GTGCCTGTCA CTCGTATTGG CTCTGACCCC AGCTTAGGTC CCCTCCAGCA AGACTCGCAC 
TTGTCTGAAG CGGAAGACTT TCACGATGCC TTGAGCACGT ACGGCACCGG TCGCTCCCCT 
AGAACTACCG CCATCATCGC CCACACGTCA TCTGGTGCGA CCGCCACTTC TACGCTGCTG 
TCCCGAAGCC AGAGCGGAGT CCCCAACGGG TACCACTACT CCCTCGGAGT GAACGCGGCT 
CCGGGATTGA GTGGGAGAGC GTCTGGAAGC TACCAGGAGA CTGAGAGAGA TGACTGGTGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_017352992.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208481.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

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CloneID	ODa56941
Clone ID Related Accession (Same CDS sequence)	XM_017352992.1
Accession Version	XM_017352992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC 
TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG 
TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA 
ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG 
ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC 
ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT 
CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC 
TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA 
GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC 
GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC 
ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG 
GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC 
GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC 
GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT 
CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC 
GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC 
CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC 
TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG 
GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG 
GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG 
ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG 
GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT 
GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG 
AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG 
CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG 
GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC 
ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC 
ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT 
ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT 
GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT 
CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC 
TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC 
AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG 
CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA 
TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG 
ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT 
GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT 
AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA 
AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC 
CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA 
CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT 
GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA 
AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG 
AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC 
CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG 
GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC 
ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT 
GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT 
GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG 
ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG 
CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG 
TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT 
TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT 
TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT 
CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC 
TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG 
TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT 
GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA 
GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG 
TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA 
CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC 
TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG 
GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG 
GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC 
ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG 
TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC 
CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA 
CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA 
GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT 
ACTGAGATCA AGACGGATAA GAGGATTAAT CTGAGTTTCC TGAGACTCTT CCGGGCGGCT 
CGTCTCATTA AACTCCTTCG GCAAGGATAC ACCATTCGGA TCCTTCTCTG GACCTTTGTT 
CAGTCCTTTA AGGCTCTTCC ATATGTGTGT CTCCTTATCG CAATGCTTTT TTTCATTTAC 
GCCATCATCG GGATGCAGGT GTTTGGTAAC ATCGCGTTGA ACAATGAAGA TTCGGCCATC 
AACATTCACA ATAACTTCCG CACCTTTTTT CAGGCCCTCA TGTTGTTGTT CAGAAGTGCT 
ACAGGGGAGG CTTGGCACGA GATCATGTTG GCTTGTCTGA GTGAAAGATA TTGCGATATA 
GAATCTGGCT ACAGTGGGAA AGAGTGCGGC AGCGATTTTG CATACTTCTA CTTCGTCTCC 
TTCATCTTTC TTTGCTCGTT TCTGATGCTG AATCTGTTTG TGGCTGTCAT CATGGATAAT 
TTTGAATATC TGACACGAGA TGCTTCTATT CTGGGGCCTC ATCACCTAGA TGAGTTCATC 
CGCGTCTGGG CTGAGTACGA CCCTGCCGCC TGTGGACGCA TGTCCTACCT CGATATGTAC 
GAGATGCTCC GCCACATGTC CCCTCCGCTA GGTTTGGGAA AGAAATGTCC TCCACGAATC 
GCCTACAAGC GATTGGTGAG GATGAACATG CCCATAGCCG AGGACAACAC GGTTCACTTT 
ACCTCCACTT TAATGGCTCT CATCCGGACT GCCCTGGAGA TCAAACTGGC ATCAGGAGTT 
CTGGCTCAGC GCATTTGTGA TGCTGAATTA AGGAAGGAAA TCAATAAAAT TTGGCCCAGT 
TTGTCATCTA AAACAGTAGA CCTGCTGGTT ACACCTCATA AACATAACGA ATTGACTGTC 
GGGAAGGTCT ATGCAGCGCT CATGATTTTT GACTACTATA AGCAGAACAG AGCCAAAAGA 
CTCCAACAGC AGCAAGCTGC ACCAGGCACA CAGAGTAAAG TCGGTGTTCT CTTCAGGCCG 
ATGCTTCCTC TCACACACCT GCATGACCAG GAGCCTTTAG TGCCAAAAGG CCCGCCTCCT 
TCTCATTTAG ATATCACCCC TCAGTCAGAT GCCACACCCA CTTCTTTTCA CTTGACCAAT 
GGGGATGCAG TTCAAAGCCA GAGCAGTGCC ATGAAAAAGT CTCCTTCGGG AGGCCCATCT 
CAGGAAGTCA CACAGGAAAC GAACAGAAGG CCTATCCAAC GTGGTCAATC GGAAGACGTG 
CCAAACACTT ACAGATCTCA GGAACTAGTA GAGATGACTG ACATGGAGAA TGATTCGGAT 
GTTGGCGGGT ATCATGTGCT GGAGGGTCAT GGCAGAGCTG CCTCTATGCC CAGACTGAAT 
GCTGGCTTCC AGCGGAGCCA CTTACGCCAC ACCGCCGGAG CGTATCTAGC ACCAATTGCA 
GATGTGAGTC CCATGCGGCG ATCCACATCC TCGCTGACTC CACAAAGAGG TGCGCGAGAG 
GTCAACCTGA GCGAATACGA CCTGGAGAGA CCCAGGATGA GCCCGAGCCA GACTTTCGCT 
CAGGACAGAG AGAGAGAGAG AGTCCACCAT CATCATCACC ACCATCATCA TCGCTGTCAG 
AGACGCAGAG ACAAGAAGCA CAAGTCACTG GATCGAGCCA TCAGCGAGGA GCAGCCGCCA 
CCTGCAGCTG ATCCAAACAC AGAGTCGACG CCCAGGGAAC GAGCCCGGGA GCGAGACCGT 
GGCCGCTCAC ACGAGAGGAA GCACCACTCA TCCTCAGCAG CAGAAAGGAA GCAGCGATAC 
TACTCTTGTG ACCGCTACGG CAGCCATGAA CATGGCCACA GCCTGTCCGC CGGGCCTAGC 
CGATCCACCT CTCCTGGAGA ACCACATGAC AGGCTTAAAC AGGGAATTAA CGCAGCTAAA 
GGAAGCTCAG TGGGTCATAC CTCAGGCACC AGCACACCCT GCCGCGGGCG TAGACAGCTT 
CCCCAGACAC CTCTTACCCC TCGGCCTGCT GTTGCCTATA AGACTGCCAA CTCTTCTCCG 
GTCCAGCTCA ACACTGCCCA ATCTACAGGG CCCTGCCCCG CTCGCCTTAG TCGTGGCCTG 
TCAGAGCACG ATACCTTACT CAGCGGTAGC ATCCACCGCC AATCCCCTGT GCCTGTCACT 
CGTATTGGCT CTGACCCCAG CTTAGGTCCC CTCCAGCAAG ACTCGCACTT GTCTGAAGCG 
GAAGACTTTC ACGATGCCTT GAGCACGTAC GGCACCGGTC GCTCCCCTAG AACTACCGCC 
ATCATCGCCC ACACGTCATC TGGTGCGACC GCCACTTCTA CGCTGCTGTC CCGAAGCCAG 
AGCGGAGTCC CCAACGGGTA CCACTACTCC CTCGGAGTGA ACGCGGCTCC GGGATTGAGT 
GGGAGAGCGT CTGGAAGCTA CCAGGAGACT GAGAGAGATG ACTGGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208481.1
CDS	87..6737
Translation	MSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKTDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352992.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352992.1

ORF Insert Sequence:

ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC 
TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG 
TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA 
ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG 
ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC 
ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT 
CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC 
TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA 
GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC 
GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC 
ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG 
GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC 
GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC 
GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT 
CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC 
GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC 
CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC 
TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG 
GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG 
GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG 
ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG 
GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT 
GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG 
AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG 
CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG 
GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC 
ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC 
ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT 
ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT 
GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT 
CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC 
TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC 
AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG 
CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA 
TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG 
ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT 
GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT 
AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA 
AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC 
CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA 
CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT 
GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA 
AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG 
AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC 
CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG 
GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC 
ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT 
GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT 
GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG 
ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG 
CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG 
TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT 
TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT 
TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT 
CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC 
TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG 
TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT 
GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA 
GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG 
TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA 
CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC 
TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG 
GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG 
GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC 
ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG 
TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC 
CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA 
CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA 
GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT 
ACTGAGATCA AGACGGATAA GAGGATTAAT CTGAGTTTCC TGAGACTCTT CCGGGCGGCT 
CGTCTCATTA AACTCCTTCG GCAAGGATAC ACCATTCGGA TCCTTCTCTG GACCTTTGTT 
CAGTCCTTTA AGGCTCTTCC ATATGTGTGT CTCCTTATCG CAATGCTTTT TTTCATTTAC 
GCCATCATCG GGATGCAGGT GTTTGGTAAC ATCGCGTTGA ACAATGAAGA TTCGGCCATC 
AACATTCACA ATAACTTCCG CACCTTTTTT CAGGCCCTCA TGTTGTTGTT CAGAAGTGCT 
ACAGGGGAGG CTTGGCACGA GATCATGTTG GCTTGTCTGA GTGAAAGATA TTGCGATATA 
GAATCTGGCT ACAGTGGGAA AGAGTGCGGC AGCGATTTTG CATACTTCTA CTTCGTCTCC 
TTCATCTTTC TTTGCTCGTT TCTGATGCTG AATCTGTTTG TGGCTGTCAT CATGGATAAT 
TTTGAATATC TGACACGAGA TGCTTCTATT CTGGGGCCTC ATCACCTAGA TGAGTTCATC 
CGCGTCTGGG CTGAGTACGA CCCTGCCGCC TGTGGACGCA TGTCCTACCT CGATATGTAC 
GAGATGCTCC GCCACATGTC CCCTCCGCTA GGTTTGGGAA AGAAATGTCC TCCACGAATC 
GCCTACAAGC GATTGGTGAG GATGAACATG CCCATAGCCG AGGACAACAC GGTTCACTTT 
ACCTCCACTT TAATGGCTCT CATCCGGACT GCCCTGGAGA TCAAACTGGC ATCAGGAGTT 
CTGGCTCAGC GCATTTGTGA TGCTGAATTA AGGAAGGAAA TCAATAAAAT TTGGCCCAGT 
TTGTCATCTA AAACAGTAGA CCTGCTGGTT ACACCTCATA AACATAACGA ATTGACTGTC 
GGGAAGGTCT ATGCAGCGCT CATGATTTTT GACTACTATA AGCAGAACAG AGCCAAAAGA 
CTCCAACAGC AGCAAGCTGC ACCAGGCACA CAGAGTAAAG TCGGTGTTCT CTTCAGGCCG 
ATGCTTCCTC TCACACACCT GCATGACCAG GAGCCTTTAG TGCCAAAAGG CCCGCCTCCT 
TCTCATTTAG ATATCACCCC TCAGTCAGAT GCCACACCCA CTTCTTTTCA CTTGACCAAT 
GGGGATGCAG TTCAAAGCCA GAGCAGTGCC ATGAAAAAGT CTCCTTCGGG AGGCCCATCT 
CAGGAAGTCA CACAGGAAAC GAACAGAAGG CCTATCCAAC GTGGTCAATC GGAAGACGTG 
CCAAACACTT ACAGATCTCA GGAACTAGTA GAGATGACTG ACATGGAGAA TGATTCGGAT 
GTTGGCGGGT ATCATGTGCT GGAGGGTCAT GGCAGAGCTG CCTCTATGCC CAGACTGAAT 
GCTGGCTTCC AGCGGAGCCA CTTACGCCAC ACCGCCGGAG CGTATCTAGC ACCAATTGCA 
GATGTGAGTC CCATGCGGCG ATCCACATCC TCGCTGACTC CACAAAGAGG TGCGCGAGAG 
GTCAACCTGA GCGAATACGA CCTGGAGAGA CCCAGGATGA GCCCGAGCCA GACTTTCGCT 
CAGGACAGAG AGAGAGAGAG AGTCCACCAT CATCATCACC ACCATCATCA TCGCTGTCAG 
AGACGCAGAG ACAAGAAGCA CAAGTCACTG GATCGAGCCA TCAGCGAGGA GCAGCCGCCA 
CCTGCAGCTG ATCCAAACAC AGAGTCGACG CCCAGGGAAC GAGCCCGGGA GCGAGACCGT 
GGCCGCTCAC ACGAGAGGAA GCACCACTCA TCCTCAGCAG CAGAAAGGAA GCAGCGATAC 
TACTCTTGTG ACCGCTACGG CAGCCATGAA CATGGCCACA GCCTGTCCGC CGGGCCTAGC 
CGATCCACCT CTCCTGGAGA ACCACATGAC AGGCTTAAAC AGGGAATTAA CGCAGCTAAA 
GGAAGCTCAG TGGGTCATAC CTCAGGCACC AGCACACCCT GCCGCGGGCG TAGACAGCTT 
CCCCAGACAC CTCTTACCCC TCGGCCTGCT GTTGCCTATA AGACTGCCAA CTCTTCTCCG 
GTCCAGCTCA ACACTGCCCA ATCTACAGGG CCCTGCCCCG CTCGCCTTAG TCGTGGCCTG 
TCAGAGCACG ATACCTTACT CAGCGGTAGC ATCCACCGCC AATCCCCTGT GCCTGTCACT 
CGTATTGGCT CTGACCCCAG CTTAGGTCCC CTCCAGCAAG ACTCGCACTT GTCTGAAGCG 
GAAGACTTTC ACGATGCCTT GAGCACGTAC GGCACCGGTC GCTCCCCTAG AACTACCGCC 
ATCATCGCCC ACACGTCATC TGGTGCGACC GCCACTTCTA CGCTGCTGTC CCGAAGCCAG 
AGCGGAGTCC CCAACGGGTA CCACTACTCC CTCGGAGTGA ACGCGGCTCC GGGATTGAGT 
GGGAGAGCGT CTGGAAGCTA CCAGGAGACT GAGAGAGATG ACTGGTGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_017352993.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208482.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

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CloneID	ODa56942
Clone ID Related Accession (Same CDS sequence)	XM_017352993.1
Accession Version	XM_017352993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC 
TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG 
TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA 
ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG 
ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC 
ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT 
CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC 
TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA 
GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC 
GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC 
ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG 
GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC 
GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC 
GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT 
CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC 
GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC 
CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC 
TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG 
GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG 
GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG 
ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG 
GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT 
GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG 
AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG 
CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG 
GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC 
ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC 
ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT 
ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT 
GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT 
CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC 
TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC 
AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG 
CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA 
TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG 
ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT 
GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT 
AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA 
AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC 
CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA 
CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT 
GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA 
AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG 
AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC 
CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG 
GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC 
ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT 
GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT 
GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG 
ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG 
CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG 
TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT 
TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT 
TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT 
CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC 
TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG 
TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT 
GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA 
GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG 
TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA 
CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC 
TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG 
GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG 
GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC 
ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG 
TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC 
CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA 
CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA 
GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT 
ACTGAGATCA AGGATAAGAG GATTAATCTG AGTTTCCTGA GACTCTTCCG GGCGGCTCGT 
CTCATTAAAC TCCTTCGGCA AGGATACACC ATTCGGATCC TTCTCTGGAC CTTTGTTCAG 
TCCTTTAAGG CTCTTCCATA TGTGTGTCTC CTTATCGCAA TGCTTTTTTT CATTTACGCC 
ATCATCGGGA TGCAGGTGTT TGGTAACATC GCGTTGAACA ATGAAGATTC GGCCATCAAC 
ATTCACAATA ACTTCCGCAC CTTTTTTCAG GCCCTCATGT TGTTGTTCAG AAGTGCTACA 
GGGGAGGCTT GGCACGAGAT CATGTTGGCT TGTCTGAGTG AAAGATATTG CGATATAGAA 
TCTGGCTACA GTGGGAAAGA GTGCGGCAGC GATTTTGCAT ACTTCTACTT CGTCTCCTTC 
ATCTTTCTTT GCTCGTTTCT GATGCTGAAT CTGTTTGTGG CTGTCATCAT GGATAATTTT 
GAATATCTGA CACGAGATGC TTCTATTCTG GGGCCTCATC ACCTAGATGA GTTCATCCGC 
GTCTGGGCTG AGTACGACCC TGCCGCCTGT GGACGCATGT CCTACCTCGA TATGTACGAG 
ATGCTCCGCC ACATGTCCCC TCCGCTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC 
TACAAGCGAT TGGTGAGGAT GAACATGCCC ATAGCCGAGG ACAACACGGT TCACTTTACC 
TCCACTTTAA TGGCTCTCAT CCGGACTGCC CTGGAGATCA AACTGGCATC AGGAGTTCTG 
GCTCAGCGCA TTTGTGATGC TGAATTAAGG AAGGAAATCA ATAAAATTTG GCCCAGTTTG 
TCATCTAAAA CAGTAGACCT GCTGGTTACA CCTCATAAAC ATAACGAATT GACTGTCGGG 
AAGGTCTATG CAGCGCTCAT GATTTTTGAC TACTATAAGC AGAACAGAGC CAAAAGACTC 
CAACAGCAGC AAGCTGCACC AGGCACACAG AGTAAAGTCG GTGTTCTCTT CAGGCCGATG 
CTTCCTCTCA CACACCTGCA TGACCAGGAG CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT 
CATTTAGATA TCACCCCTCA GTCAGATGCC ACACCCACTT CTTTTCACTT GACCAATGGG 
GATGCAGTTC AAAGCCAGAG CAGTGCCATG AAAAAGTCTC CTTCGGGAGG CCCATCTCAG 
GAAGTCACAC AGGAAACGAA CAGAAGGCCT ATCCAACGTG GTCAATCGGA AGACGTGCCA 
AACACTTACA GATCTCAGGA ACTAGTAGAG ATGACTGACA TGGAGAATGA TTCGGATGTT 
GGCGGGTATC ATGTGCTGGA GGGTCATGGC AGAGCTGCCT CTATGCCCAG ACTGAATGCT 
GGCTTCCAGC GGAGCCACTT ACGCCACACC GCCGGAGCGT ATCTAGCACC AATTGCAGAT 
GTGAGTCCCA TGCGGCGATC CACATCCTCG CTGACTCCAC AAAGAGGTGC GCGAGAGGTC 
AACCTGAGCG AATACGACCT GGAGAGACCC AGGATGAGCC CGAGCCAGAC TTTCGCTCAG 
GACAGAGAGA GAGAGAGAGT CCACCATCAT CATCACCACC ATCATCATCG CTGTCAGAGA 
CGCAGAGACA AGAAGCACAA GTCACTGGAT CGAGCCATCA GCGAGGAGCA GCCGCCACCT 
GCAGCTGATC CAAACACAGA GTCGACGCCC AGGGAACGAG CCCGGGAGCG AGACCGTGGC 
CGCTCACACG AGAGGAAGCA CCACTCATCC TCAGCAGCAG AAAGGAAGCA GCGATACTAC 
TCTTGTGACC GCTACGGCAG CCATGAACAT GGCCACAGCC TGTCCGCCGG GCCTAGCCGA 
TCCACCTCTC CTGGAGAACC ACATGACAGG CTTAAACAGG GAATTAACGC AGCTAAAGGA 
AGCTCAGTGG GTCATACCTC AGGCACCAGC ACACCCTGCC GCGGGCGTAG ACAGCTTCCC 
CAGACACCTC TTACCCCTCG GCCTGCTGTT GCCTATAAGA CTGCCAACTC TTCTCCGGTC 
CAGCTCAACA CTGCCCAATC TACAGGGCCC TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA 
GAGCACGATA CCTTACTCAG CGGTAGCATC CACCGCCAAT CCCCTGTGCC TGTCACTCGT 
ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC CAGCAAGACT CGCACTTGTC TGAAGCGGAA 
GACTTTCACG ATGCCTTGAG CACGTACGGC ACCGGTCGCT CCCCTAGAAC TACCGCCATC 
ATCGCCCACA CGTCATCTGG TGCGACCGCC ACTTCTACGC TGCTGTCCCG AAGCCAGAGC 
GGAGTCCCCA ACGGGTACCA CTACTCCCTC GGAGTGAACG CGGCTCCGGG ATTGAGTGGG 
AGAGCGTCTG GAAGCTACCA GGAGACTGAG AGAGATGACT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208482.1
CDS	87..6734
Translation	MSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRMSYLDMYEMLRHMSPPLGLGKKCPPRIAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352993.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352993.1

ORF Insert Sequence:

ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC 
TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG 
TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA 
ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG 
ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC 
ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT 
CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC 
TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA 
GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC 
GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC 
ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG 
GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC 
GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC 
GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT 
CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC 
GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC 
CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC 
TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG 
GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG 
GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG 
ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG 
GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT 
GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG 
AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG 
CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG 
GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC 
ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC 
ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT 
ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT 
GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT 
CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC 
TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC 
AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG 
CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA 
TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG 
ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT 
GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT 
AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA 
AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC 
CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA 
CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT 
GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA 
AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG 
AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC 
CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG 
GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC 
ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT 
GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT 
GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG 
ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG 
CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG 
TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT 
TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT 
TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT 
CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC 
TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG 
TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT 
GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA 
GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG 
TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA 
CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC 
TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG 
GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG 
GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC 
ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG 
TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC 
CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA 
CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA 
GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT 
ACTGAGATCA AGGATAAGAG GATTAATCTG AGTTTCCTGA GACTCTTCCG GGCGGCTCGT 
CTCATTAAAC TCCTTCGGCA AGGATACACC ATTCGGATCC TTCTCTGGAC CTTTGTTCAG 
TCCTTTAAGG CTCTTCCATA TGTGTGTCTC CTTATCGCAA TGCTTTTTTT CATTTACGCC 
ATCATCGGGA TGCAGGTGTT TGGTAACATC GCGTTGAACA ATGAAGATTC GGCCATCAAC 
ATTCACAATA ACTTCCGCAC CTTTTTTCAG GCCCTCATGT TGTTGTTCAG AAGTGCTACA 
GGGGAGGCTT GGCACGAGAT CATGTTGGCT TGTCTGAGTG AAAGATATTG CGATATAGAA 
TCTGGCTACA GTGGGAAAGA GTGCGGCAGC GATTTTGCAT ACTTCTACTT CGTCTCCTTC 
ATCTTTCTTT GCTCGTTTCT GATGCTGAAT CTGTTTGTGG CTGTCATCAT GGATAATTTT 
GAATATCTGA CACGAGATGC TTCTATTCTG GGGCCTCATC ACCTAGATGA GTTCATCCGC 
GTCTGGGCTG AGTACGACCC TGCCGCCTGT GGACGCATGT CCTACCTCGA TATGTACGAG 
ATGCTCCGCC ACATGTCCCC TCCGCTAGGT TTGGGAAAGA AATGTCCTCC ACGAATCGCC 
TACAAGCGAT TGGTGAGGAT GAACATGCCC ATAGCCGAGG ACAACACGGT TCACTTTACC 
TCCACTTTAA TGGCTCTCAT CCGGACTGCC CTGGAGATCA AACTGGCATC AGGAGTTCTG 
GCTCAGCGCA TTTGTGATGC TGAATTAAGG AAGGAAATCA ATAAAATTTG GCCCAGTTTG 
TCATCTAAAA CAGTAGACCT GCTGGTTACA CCTCATAAAC ATAACGAATT GACTGTCGGG 
AAGGTCTATG CAGCGCTCAT GATTTTTGAC TACTATAAGC AGAACAGAGC CAAAAGACTC 
CAACAGCAGC AAGCTGCACC AGGCACACAG AGTAAAGTCG GTGTTCTCTT CAGGCCGATG 
CTTCCTCTCA CACACCTGCA TGACCAGGAG CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT 
CATTTAGATA TCACCCCTCA GTCAGATGCC ACACCCACTT CTTTTCACTT GACCAATGGG 
GATGCAGTTC AAAGCCAGAG CAGTGCCATG AAAAAGTCTC CTTCGGGAGG CCCATCTCAG 
GAAGTCACAC AGGAAACGAA CAGAAGGCCT ATCCAACGTG GTCAATCGGA AGACGTGCCA 
AACACTTACA GATCTCAGGA ACTAGTAGAG ATGACTGACA TGGAGAATGA TTCGGATGTT 
GGCGGGTATC ATGTGCTGGA GGGTCATGGC AGAGCTGCCT CTATGCCCAG ACTGAATGCT 
GGCTTCCAGC GGAGCCACTT ACGCCACACC GCCGGAGCGT ATCTAGCACC AATTGCAGAT 
GTGAGTCCCA TGCGGCGATC CACATCCTCG CTGACTCCAC AAAGAGGTGC GCGAGAGGTC 
AACCTGAGCG AATACGACCT GGAGAGACCC AGGATGAGCC CGAGCCAGAC TTTCGCTCAG 
GACAGAGAGA GAGAGAGAGT CCACCATCAT CATCACCACC ATCATCATCG CTGTCAGAGA 
CGCAGAGACA AGAAGCACAA GTCACTGGAT CGAGCCATCA GCGAGGAGCA GCCGCCACCT 
GCAGCTGATC CAAACACAGA GTCGACGCCC AGGGAACGAG CCCGGGAGCG AGACCGTGGC 
CGCTCACACG AGAGGAAGCA CCACTCATCC TCAGCAGCAG AAAGGAAGCA GCGATACTAC 
TCTTGTGACC GCTACGGCAG CCATGAACAT GGCCACAGCC TGTCCGCCGG GCCTAGCCGA 
TCCACCTCTC CTGGAGAACC ACATGACAGG CTTAAACAGG GAATTAACGC AGCTAAAGGA 
AGCTCAGTGG GTCATACCTC AGGCACCAGC ACACCCTGCC GCGGGCGTAG ACAGCTTCCC 
CAGACACCTC TTACCCCTCG GCCTGCTGTT GCCTATAAGA CTGCCAACTC TTCTCCGGTC 
CAGCTCAACA CTGCCCAATC TACAGGGCCC TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA 
GAGCACGATA CCTTACTCAG CGGTAGCATC CACCGCCAAT CCCCTGTGCC TGTCACTCGT 
ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC CAGCAAGACT CGCACTTGTC TGAAGCGGAA 
GACTTTCACG ATGCCTTGAG CACGTACGGC ACCGGTCGCT CCCCTAGAAC TACCGCCATC 
ATCGCCCACA CGTCATCTGG TGCGACCGCC ACTTCTACGC TGCTGTCCCG AAGCCAGAGC 
GGAGTCCCCA ACGGGTACCA CTACTCCCTC GGAGTGAACG CGGCTCCGGG ATTGAGTGGG 
AGAGCGTCTG GAAGCTACCA GGAGACTGAG AGAGATGACT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna1bb ( XM_017352994.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208483.1 Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

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CloneID	ODa56943
Clone ID Related Accession (Same CDS sequence)	XM_017352994.1
Accession Version	XM_017352994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent N-type calcium channel subunit alpha-1B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC 
TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG 
TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA 
ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG 
ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC 
ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT 
CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC 
TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA 
GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC 
GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC 
ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG 
GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC 
GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC 
GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT 
CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC 
GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC 
CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC 
TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG 
GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG 
GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG 
ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG 
GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT 
GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG 
AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG 
CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG 
GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC 
ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC 
ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT 
ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT 
GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT 
CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC 
TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC 
AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG 
CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA 
TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG 
ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT 
GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT 
AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA 
AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC 
CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA 
CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT 
GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA 
AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG 
AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC 
CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG 
GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC 
ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT 
GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT 
GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG 
ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG 
CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG 
TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT 
TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT 
TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT 
CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC 
TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG 
TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT 
GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA 
GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG 
TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA 
CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC 
TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG 
GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG 
GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC 
ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG 
TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC 
CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA 
CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA 
GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT 
ACTGAGATCA AGGATAAGAG GATTAATCTG AGTTTCCTGA GACTCTTCCG GGCGGCTCGT 
CTCATTAAAC TCCTTCGGCA AGGATACACC ATTCGGATCC TTCTCTGGAC CTTTGTTCAG 
TCCTTTAAGG CTCTTCCATA TGTGTGTCTC CTTATCGCAA TGCTTTTTTT CATTTACGCC 
ATCATCGGGA TGCAGGTGTT TGGTAACATC GCGTTGAACA ATGAAGATTC GGCCATCAAC 
ATTCACAATA ACTTCCGCAC CTTTTTTCAG GCCCTCATGT TGTTGTTCAG AAGTGCTACA 
GGGGAGGCTT GGCACGAGAT CATGTTGGCT TGTCTGAGTG AAAGATATTG CGATATAGAA 
TCTGGCTACA GTGGGAAAGA GTGCGGCAGC GATTTTGCAT ACTTCTACTT CGTCTCCTTC 
ATCTTTCTTT GCTCGTTTCT GATGCTGAAT CTGTTTGTGG CTGTCATCAT GGATAATTTT 
GAATATCTGA CACGAGATGC TTCTATTCTG GGGCCTCATC ACCTAGATGA GTTCATCCGC 
GTCTGGGCTG AGTACGACCC TGCCGCCTGT GGAAGGATTC CCTATATGGA TATGTACAAT 
TTGTTGCGTG TAATCTCACC TCCCCTTGGC TTAGGAAAGA ACTGCCCTAA TAGGGTGGCA 
TACAAGCGAT TGGTGAGGAT GAACATGCCC ATAGCCGAGG ACAACACGGT TCACTTTACC 
TCCACTTTAA TGGCTCTCAT CCGGACTGCC CTGGAGATCA AACTGGCATC AGGAGTTCTG 
GCTCAGCGCA TTTGTGATGC TGAATTAAGG AAGGAAATCA ATAAAATTTG GCCCAGTTTG 
TCATCTAAAA CAGTAGACCT GCTGGTTACA CCTCATAAAC ATAACGAATT GACTGTCGGG 
AAGGTCTATG CAGCGCTCAT GATTTTTGAC TACTATAAGC AGAACAGAGC CAAAAGACTC 
CAACAGCAGC AAGCTGCACC AGGCACACAG AGTAAAGTCG GTGTTCTCTT CAGGCCGATG 
CTTCCTCTCA CACACCTGCA TGACCAGGAG CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT 
CATTTAGATA TCACCCCTCA GTCAGATGCC ACACCCACTT CTTTTCACTT GACCAATGGG 
GATGCAGTTC AAAGCCAGAG CAGTGCCATG AAAAAGTCTC CTTCGGGAGG CCCATCTCAG 
GAAGTCACAC AGGAAACGAA CAGAAGGCCT ATCCAACGTG GTCAATCGGA AGACGTGCCA 
AACACTTACA GATCTCAGGA ACTAGTAGAG ATGACTGACA TGGAGAATGA TTCGGATGTT 
GGCGGGTATC ATGTGCTGGA GGGTCATGGC AGAGCTGCCT CTATGCCCAG ACTGAATGCT 
GGCTTCCAGC GGAGCCACTT ACGCCACACC GCCGGAGCGT ATCTAGCACC AATTGCAGAT 
GTGAGTCCCA TGCGGCGATC CACATCCTCG CTGACTCCAC AAAGAGGTGC GCGAGAGGTC 
AACCTGAGCG AATACGACCT GGAGAGACCC AGGATGAGCC CGAGCCAGAC TTTCGCTCAG 
GACAGAGAGA GAGAGAGAGT CCACCATCAT CATCACCACC ATCATCATCG CTGTCAGAGA 
CGCAGAGACA AGAAGCACAA GTCACTGGAT CGAGCCATCA GCGAGGAGCA GCCGCCACCT 
GCAGCTGATC CAAACACAGA GTCGACGCCC AGGGAACGAG CCCGGGAGCG AGACCGTGGC 
CGCTCACACG AGAGGAAGCA CCACTCATCC TCAGCAGCAG AAAGGAAGCA GCGATACTAC 
TCTTGTGACC GCTACGGCAG CCATGAACAT GGCCACAGCC TGTCCGCCGG GCCTAGCCGA 
TCCACCTCTC CTGGAGAACC ACATGACAGG CTTAAACAGG GAATTAACGC AGCTAAAGGA 
AGCTCAGTGG GTCATACCTC AGGCACCAGC ACACCCTGCC GCGGGCGTAG ACAGCTTCCC 
CAGACACCTC TTACCCCTCG GCCTGCTGTT GCCTATAAGA CTGCCAACTC TTCTCCGGTC 
CAGCTCAACA CTGCCCAATC TACAGGGCCC TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA 
GAGCACGATA CCTTACTCAG CGGTAGCATC CACCGCCAAT CCCCTGTGCC TGTCACTCGT 
ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC CAGCAAGACT CGCACTTGTC TGAAGCGGAA 
GACTTTCACG ATGCCTTGAG CACGTACGGC ACCGGTCGCT CCCCTAGAAC TACCGCCATC 
ATCGCCCACA CGTCATCTGG TGCGACCGCC ACTTCTACGC TGCTGTCCCG AAGCCAGAGC 
GGAGTCCCCA ACGGGTACCA CTACTCCCTC GGAGTGAACG CGGCTCCGGG ATTGAGTGGG 
AGAGCGTCTG GAAGCTACCA GGAGACTGAG AGAGATGACT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208483.1
CDS	87..6734
Translation	MSERLDDTEPYFIGIFCFEAGIKIIALGFVFHKDSYLRNGWNIMDFVVVVTGILTTVGSQFDLRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAIVMFAIIGVEFYMGKFHFTCFKVDTGEKAVDYPCGAEPPSRSCPVGTNCTEYWKGPNFGITNFDNILFAILTVFQCITMEGWTDVLYHTNDAAGNTWNWLYFIPLIIIGSFFMLNLVLGVLSGEFAKERERVEKRQEFLKLRRQQQIERELTGYLEWICKAEEVLLAEEDQNAEEKSPLDGAWYRRKQNNPVLKRTKSRKGRNDLISAEEGDEHFTDISSVAPPGSPFARASLKSSKNDSSSYFRRKEKRIRFFIRRMVKAQSFYWIVLCLVGLNTMCVAIVHYDQPEWLTKALYLAEFVFLGLFLTEMTLKMYGLGPRNYFHSSFNCFDFGVIVGSIFEVIWAAIQPGASFGISVLRALRLLRIFKVTKYWNSLRNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFEDETPTTNFDSFPAAIMTVFQILTGEDWNAVMYHGIESQGGVRGGMFSSVYFIVLTLFGNYTLLNVFLAIAVDNLANAQELTKDEEQQEEAAKKSLALQKAMEVKEVSPMSAANISIAAFVKQNRDAISRRSSVCSVHSPKEQQRSLQKLSIWEQRTNQLRRHNLRSSSEALYNELEPEERLRVSSALHLRPDMKTHHDRPLVVEHRDSVDNLRSDKDGDTSEGQPASNHQRKYHKCRGENGDTGEGRLHRHHSQNRENQGGLERSNSQDGGQRNHHRSSGSPEEGIGIEEREHRHHHSRRPREVNGNGNGVIGNGGRGERIRGHREGEGGNGERRRHRPRVKAQSTLDGDECRENGGKRRSRPTETDSLTTSPLQSPSEEKPEDKDNLKNSTRVGPSGVNIPVTITAPPGETTVIPMNNIDGDSLLLNEEKKDLDELNQNAPKHILPYSSMFVFGPTNPVRRLCHYVVNLRYFEMCILTVITMSSIALAAEDPVQAHAPRNDVLKYLDYVFTGVFTFEMVIKMIDLGLLLHPGSYFRDLWNILDFIVVSGALVAFACSGTKGKDINTIKSLRVLRVLRPLKTIKRLPKLKAVFDCVVNSLKNVLNILIVYILFMFIFAVIAVQLFKGKFFHCTDESKALEKDCRGQFLEYGSDGVAMTQPREWKKYDFHYDNVLWAFLTLFTVSTGEGWPLVLKHSVDATYEDQGPSPGYRMETSIFYVVYFIVFPFFFVNIFVALIIITFQEQGDKAMSECSLEKNERACIDFAINAKPLTRYMPQDKNSYQYKMWKFVVSTPFEYSILTMIAINTVVLMMKFHGAPKPYEDMLKWLNIIFTALFTLECVLKVIAFGPLNYLKEAWNIFDFVTVLGSITDILVTEIKDKRINLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIYAIIGMQVFGNIALNNEDSAINIHNNFRTFFQALMLLFRSATGEAWHEIMLACLSERYCDIESGYSGKECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNFEYLTRDASILGPHHLDEFIRVWAEYDPAACGRIPYMDMYNLLRVISPPLGLGKNCPNRVAYKRLVRMNMPIAEDNTVHFTSTLMALIRTALEIKLASGVLAQRICDAELRKEINKIWPSLSSKTVDLLVTPHKHNELTVGKVYAALMIFDYYKQNRAKRLQQQQAAPGTQSKVGVLFRPMLPLTHLHDQEPLVPKGPPPSHLDITPQSDATPTSFHLTNGDAVQSQSSAMKKSPSGGPSQEVTQETNRRPIQRGQSEDVPNTYRSQELVEMTDMENDSDVGGYHVLEGHGRAASMPRLNAGFQRSHLRHTAGAYLAPIADVSPMRRSTSSLTPQRGAREVNLSEYDLERPRMSPSQTFAQDRERERVHHHHHHHHHRCQRRRDKKHKSLDRAISEEQPPPAADPNTESTPRERARERDRGRSHERKHHSSSAAERKQRYYSCDRYGSHEHGHSLSAGPSRSTSPGEPHDRLKQGINAAKGSSVGHTSGTSTPCRGRRQLPQTPLTPRPAVAYKTANSSPVQLNTAQSTGPCPARLSRGLSEHDTLLSGSIHRQSPVPVTRIGSDPSLGPLQQDSHLSEAEDFHDALSTYGTGRSPRTTAIIAHTSSGATATSTLLSRSQSGVPNGYHYSLGVNAAPGLSGRASGSYQETERDDWC

Target ORF information:

RefSeq Version	XM_017352994.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, N type, alpha 1B subunit, b (cacna1bb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352994.1

ORF Insert Sequence:

ATGTCTGAAC GCCTGGACGA CACAGAGCCC TACTTTATCG GAATATTCTG TTTCGAGGCC 
GGCATTAAGA TCATCGCCTT AGGCTTCGTG TTTCACAAAG ACTCTTACCT TCGCAACGGC 
TGGAACATAA TGGACTTTGT GGTCGTTGTG ACTGGGATTT TGACCACAGT GGGTTCACAG 
TTCGACTTGA GGACGTTGAG GGCCGTTAGA GTGCTGAGAC CCCTCAAACT GGTGTCAGGA 
ATACCAAGTC TTCAAGTAGT ATTAAAGTCC ATCATGAAGG CCATGGTTCC CCTTCTTCAG 
ATCGGATTGC TGCTCTTCTT TGCCATCGTT ATGTTTGCCA TTATTGGTGT CGAGTTTTAC 
ATGGGCAAGT TCCACTTCAC CTGCTTCAAA GTGGATACAG GAGAAAAGGC TGTTGATTAT 
CCATGTGGAG CTGAGCCTCC CTCAAGATCG TGTCCCGTTG GAACAAACTG TACGGAGTAC 
TGGAAAGGAC CCAATTTTGG CATCACAAAT TTCGATAACA TCCTCTTCGC CATCCTCACA 
GTCTTCCAGT GCATCACCAT GGAAGGATGG ACTGATGTAC TATACCATAC CAATGATGCC 
GCGGGAAACA CCTGGAATTG GCTGTACTTT ATCCCTCTCA TCATTATCGG CTCTTTTTTC 
ATGCTTAACC TCGTCCTGGG TGTCTTATCA GGAGAGTTTG CCAAAGAGAG GGAGCGAGTG 
GAGAAGAGGC AGGAGTTCTT AAAGCTTCGC AGACAGCAGC AGATTGAGAG GGAGCTCACC 
GGCTACTTGG AATGGATCTG CAAAGCAGAG GAGGTGCTGC TGGCGGAGGA AGACCAGAAC 
GCTGAGGAGA AATCCCCTCT GGATGGTGCG TGGTACAGGC GGAAACAGAA CAACCCAGTT 
CTAAAAAGAA CAAAGTCCAG AAAGGGTAGA AATGACCTCA TCAGTGCTGA AGAGGGAGAC 
GAACATTTCA CAGACATCTC CTCTGTCGCA CCACCTGGAT CTCCTTTTGC CAGAGCTAGC 
CTGAAGAGCA GTAAGAACGA CAGCTCCTCA TATTTCCGTC GAAAGGAAAA AAGGATTCGC 
TTTTTCATCC GGCGGATGGT GAAGGCACAG AGTTTCTACT GGATCGTTTT GTGTTTGGTG 
GGATTGAACA CGATGTGTGT GGCTATTGTC CATTATGACC AGCCGGAGTG GCTCACCAAG 
GCCCTGTACC TGGCAGAGTT TGTATTCCTG GGTCTGTTTC TGACAGAGAT GACTCTGAAG 
ATGTACGGCC TGGGGCCCAG AAACTACTTT CACTCCTCGT TCAACTGTTT TGACTTTGGG 
GTAATTGTGG GGAGTATATT TGAGGTGATC TGGGCAGCGA TTCAGCCTGG TGCGTCTTTT 
GGAATCAGTG TTTTGAGAGC CCTGCGTCTG CTCCGTATCT TCAAAGTAAC CAAATACTGG 
AACTCTTTGC GGAATCTCGT GGTTTCTCTG TTAAACTCCA TGAAGTCCAT CATCAGCTTG 
CTCTTCCTCC TCTTCCTCTT CATCGTGGTG TTTGCTCTGC TTGGCATGCA GCTCTTCGGG 
GGCCAGTTTA ATTTTGAAGA TGAAACACCC ACTACAAATT TTGACAGCTT CCCTGCAGCC 
ATAATGACAG TCTTCCAGAT TTTGACAGGC GAGGATTGGA ACGCAGTGAT GTATCATGGC 
ATCGAGTCGC AGGGTGGCGT CCGGGGTGGG ATGTTCTCCT CGGTTTACTT CATTGTTCTT 
ACTCTCTTCG GAAACTACAC ACTTCTCAAT GTATTCTTGG CCATCGCTGT GGATAACCTT 
GCCAATGCGC AGGAGCTGAC AAAGGATGAA GAGCAGCAGG AGGAGGCAGC GAAGAAAAGT 
CTGGCTCTTC AGAAGGCCAT GGAGGTGAAA GAGGTCAGCC CCATGTCTGC TGCCAACATC 
TCCATCGCAG CTTTTGTAAA GCAAAATCGA GATGCAATCT CTCGCAGGTC TTCAGTCTGC 
AGCGTTCATT CACCTAAAGA GCAGCAGAGG TCACTGCAGA AGTTGTCCAT ATGGGAGCAG 
CGCACTAACC AACTGCGCAG ACACAACCTG CGTAGCAGCA GCGAGGCACT TTACAACGAA 
TTGGAGCCAG AAGAGCGTCT GCGTGTCTCC TCTGCCCTGC ATCTGCGGCC TGACATGAAG 
ACCCACCACG ATCGGCCGCT GGTTGTGGAG CACCGAGATT CTGTGGACAA TCTCAGATCT 
GACAAAGACG GAGACACTTC AGAGGGTCAG CCAGCATCTA ACCACCAGAG AAAATATCAT 
AAATGTCGAG GGGAGAACGG AGACACCGGC GAGGGACGGC TTCACAGACA CCACAGCCAA 
AATCGAGAGA ACCAGGGTGG CCTAGAGCGC AGCAACAGTC AAGATGGAGG ACAGAGGAAC 
CACCACAGAT CTTCTGGATC TCCAGAGGAG GGCATTGGGA TTGAGGAGAG AGAACATAGA 
CATCATCATT CTAGAAGACC CAGAGAAGTC AACGGGAACG GGAATGGTGT GATTGGTAAT 
GGAGGCAGGG GCGAGAGGAT ACGAGGTCAC AGAGAGGGTG AAGGAGGGAA TGGAGAGAGA 
AGGAGACACC GGCCACGGGT TAAAGCCCAG TCCACATTGG ATGGAGACGA ATGTAGAGAG 
AATGGTGGTA AACGCAGGAG CAGACCAACA GAAACAGACA GCCTCACAAC CAGCCCTCTC 
CAGTCTCCTT CTGAAGAGAA GCCAGAAGAC AAAGATAATC TAAAAAACAG CACTCGAGTG 
GGTCCAAGCG GAGTCAACAT CCCTGTCACC ATAACTGCGC CACCAGGAGA GACCACTGTC 
ATTCCAATGA ACAACATTGA TGGTGACAGT CTGCTGCTGA ATGAGGAAAA GAAGGATCTT 
GATGAGCTAA ATCAGAATGC GCCTAAACAC ATTTTGCCAT ACAGCTCCAT GTTTGTGTTT 
GGCCCAACCA ACCCAGTGCG ACGACTGTGT CATTATGTGG TGAATCTGCG GTATTTTGAG 
ATGTGCATCC TCACAGTCAT CACCATGAGC AGTATCGCTC TCGCTGCCGA AGACCCCGTG 
CAGGCTCATG CCCCTCGCAA TGATGTGCTG AAATACCTTG ATTACGTCTT CACTGGTGTG 
TTCACATTTG AGATGGTTAT AAAGATGATT GATCTTGGAC TGCTGCTGCA TCCCGGCTCT 
TACTTCAGGG ACCTGTGGAA CATCCTGGAC TTCATCGTAG TGAGCGGCGC TCTGGTGGCT 
TTCGCCTGCT CAGGAACTAA AGGAAAGGAC ATAAACACCA TCAAGTCCCT GAGGGTTCTT 
CGAGTCTTGA GACCCTTGAA GACAATCAAA AGATTGCCCA AACTCAAGGC CGTGTTTGAC 
TGCGTGGTGA ACTCTCTGAA GAATGTGCTC AACATCCTCA TCGTCTACAT CCTCTTCATG 
TTCATCTTCG CGGTCATCGC CGTGCAGCTT TTCAAGGGGA AGTTTTTCCA CTGCACCGAT 
GAGTCCAAAG CCCTGGAGAA GGACTGCAGG GGTCAGTTCT TGGAGTACGG CAGTGATGGA 
GTGGCCATGA CGCAGCCGCG CGAGTGGAAG AAGTACGACT TCCATTATGA CAACGTCCTG 
TGGGCCTTTC TGACCCTCTT CACTGTGTCT ACAGGAGAGG GCTGGCCACT TGTGCTGAAA 
CACTCTGTTG ATGCCACGTA TGAAGACCAG GGTCCCAGTC CAGGATATCG CATGGAAACC 
TCCATCTTCT ATGTGGTCTA CTTCATCGTC TTTCCCTTCT TCTTTGTCAA CATCTTTGTG 
GCTTTGATCA TCATCACATT CCAGGAGCAG GGGGATAAAG CCATGTCCGA GTGCAGCTTG 
GAAAAGAACG AGCGAGCTTG TATTGACTTT GCCATCAATG CTAAACCTCT GACTCGCTAC 
ATGCCGCAAG ACAAGAACTC TTACCAGTAC AAGATGTGGA AGTTTGTGGT GTCGACTCCG 
TTTGAGTACT CCATCCTGAC CATGATCGCC ATCAATACAG TGGTGCTCAT GATGAAGTTC 
CATGGTGCTC CTAAACCTTA TGAGGACATG TTGAAGTGGC TGAACATCAT CTTCACTGCA 
CTCTTCACAC TGGAGTGTGT TCTCAAGGTC ATCGCCTTCG GTCCTCTGAA CTACCTGAAA 
GAGGCTTGGA ACATTTTTGA CTTTGTTACT GTTCTGGGAA GCATCACAGA CATCCTGGTT 
ACTGAGATCA AGGATAAGAG GATTAATCTG AGTTTCCTGA GACTCTTCCG GGCGGCTCGT 
CTCATTAAAC TCCTTCGGCA AGGATACACC ATTCGGATCC TTCTCTGGAC CTTTGTTCAG 
TCCTTTAAGG CTCTTCCATA TGTGTGTCTC CTTATCGCAA TGCTTTTTTT CATTTACGCC 
ATCATCGGGA TGCAGGTGTT TGGTAACATC GCGTTGAACA ATGAAGATTC GGCCATCAAC 
ATTCACAATA ACTTCCGCAC CTTTTTTCAG GCCCTCATGT TGTTGTTCAG AAGTGCTACA 
GGGGAGGCTT GGCACGAGAT CATGTTGGCT TGTCTGAGTG AAAGATATTG CGATATAGAA 
TCTGGCTACA GTGGGAAAGA GTGCGGCAGC GATTTTGCAT ACTTCTACTT CGTCTCCTTC 
ATCTTTCTTT GCTCGTTTCT GATGCTGAAT CTGTTTGTGG CTGTCATCAT GGATAATTTT 
GAATATCTGA CACGAGATGC TTCTATTCTG GGGCCTCATC ACCTAGATGA GTTCATCCGC 
GTCTGGGCTG AGTACGACCC TGCCGCCTGT GGAAGGATTC CCTATATGGA TATGTACAAT 
TTGTTGCGTG TAATCTCACC TCCCCTTGGC TTAGGAAAGA ACTGCCCTAA TAGGGTGGCA 
TACAAGCGAT TGGTGAGGAT GAACATGCCC ATAGCCGAGG ACAACACGGT TCACTTTACC 
TCCACTTTAA TGGCTCTCAT CCGGACTGCC CTGGAGATCA AACTGGCATC AGGAGTTCTG 
GCTCAGCGCA TTTGTGATGC TGAATTAAGG AAGGAAATCA ATAAAATTTG GCCCAGTTTG 
TCATCTAAAA CAGTAGACCT GCTGGTTACA CCTCATAAAC ATAACGAATT GACTGTCGGG 
AAGGTCTATG CAGCGCTCAT GATTTTTGAC TACTATAAGC AGAACAGAGC CAAAAGACTC 
CAACAGCAGC AAGCTGCACC AGGCACACAG AGTAAAGTCG GTGTTCTCTT CAGGCCGATG 
CTTCCTCTCA CACACCTGCA TGACCAGGAG CCTTTAGTGC CAAAAGGCCC GCCTCCTTCT 
CATTTAGATA TCACCCCTCA GTCAGATGCC ACACCCACTT CTTTTCACTT GACCAATGGG 
GATGCAGTTC AAAGCCAGAG CAGTGCCATG AAAAAGTCTC CTTCGGGAGG CCCATCTCAG 
GAAGTCACAC AGGAAACGAA CAGAAGGCCT ATCCAACGTG GTCAATCGGA AGACGTGCCA 
AACACTTACA GATCTCAGGA ACTAGTAGAG ATGACTGACA TGGAGAATGA TTCGGATGTT 
GGCGGGTATC ATGTGCTGGA GGGTCATGGC AGAGCTGCCT CTATGCCCAG ACTGAATGCT 
GGCTTCCAGC GGAGCCACTT ACGCCACACC GCCGGAGCGT ATCTAGCACC AATTGCAGAT 
GTGAGTCCCA TGCGGCGATC CACATCCTCG CTGACTCCAC AAAGAGGTGC GCGAGAGGTC 
AACCTGAGCG AATACGACCT GGAGAGACCC AGGATGAGCC CGAGCCAGAC TTTCGCTCAG 
GACAGAGAGA GAGAGAGAGT CCACCATCAT CATCACCACC ATCATCATCG CTGTCAGAGA 
CGCAGAGACA AGAAGCACAA GTCACTGGAT CGAGCCATCA GCGAGGAGCA GCCGCCACCT 
GCAGCTGATC CAAACACAGA GTCGACGCCC AGGGAACGAG CCCGGGAGCG AGACCGTGGC 
CGCTCACACG AGAGGAAGCA CCACTCATCC TCAGCAGCAG AAAGGAAGCA GCGATACTAC 
TCTTGTGACC GCTACGGCAG CCATGAACAT GGCCACAGCC TGTCCGCCGG GCCTAGCCGA 
TCCACCTCTC CTGGAGAACC ACATGACAGG CTTAAACAGG GAATTAACGC AGCTAAAGGA 
AGCTCAGTGG GTCATACCTC AGGCACCAGC ACACCCTGCC GCGGGCGTAG ACAGCTTCCC 
CAGACACCTC TTACCCCTCG GCCTGCTGTT GCCTATAAGA CTGCCAACTC TTCTCCGGTC 
CAGCTCAACA CTGCCCAATC TACAGGGCCC TGCCCCGCTC GCCTTAGTCG TGGCCTGTCA 
GAGCACGATA CCTTACTCAG CGGTAGCATC CACCGCCAAT CCCCTGTGCC TGTCACTCGT 
ATTGGCTCTG ACCCCAGCTT AGGTCCCCTC CAGCAAGACT CGCACTTGTC TGAAGCGGAA 
GACTTTCACG ATGCCTTGAG CACGTACGGC ACCGGTCGCT CCCCTAGAAC TACCGCCATC 
ATCGCCCACA CGTCATCTGG TGCGACCGCC ACTTCTACGC TGCTGTCCCG AAGCCAGAGC 
GGAGTCCCCA ACGGGTACCA CTACTCCCTC GGAGTGAACG CGGCTCCGGG ATTGAGTGGG 
AGAGCGTCTG GAAGCTACCA GGAGACTGAG AGAGATGACT GGTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	cacna1bb
Entrez Gene ID	562796
Full Name	calcium channel, voltage-dependent, N type, alpha 1B subunit, b
Synonyms	si:dkeyp-23f6.1,si:dkeyp-23f6.2
General protein information	Preferred Names calcium channel, voltage-dependent, N type, alpha 1B subunit, b Names voltage-dependent N-type calcium channel subunit alpha-1B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Pan troglodytes (chimpanzee)	CACNA1B	XP_520396.4
Canis lupus familiaris (dog)	CACNA1B	XP_005625483.1
Rattus norvegicus (Norway rat)	Cacna1b	NP_671482.1
Danio rerio (zebrafish)	cacna1ba	NP_001108020.1
Caenorhabditis elegans (roundworm)	unc-2	NP_001123176.1
Gallus gallus (chicken)	CACNA1B	NP_989624.1
Drosophila melanogaster (fruit fly)	cac	NP_001245636.1
Homo sapiens (human)	CACNA1B	NP_000709.1
Bos taurus (cattle)	CACNA1B	NP_777057.1
Mus musculus (house mouse)	Cacna1b	NP_001035993.1
Danio rerio (zebrafish)	cacna1bb	XP_002665969.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002578	XP_312358.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

cacna1bb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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