sp5a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sp5a
Entrez Gene ID	353154
Full Name	Sp5 transcription factor a
Synonyms	bts1,fc39b01,sp5,wu:fc39b01
General protein information	Preferred Names Sp5 transcription factor a Names transcription factor Sp5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_180973.3	NP_851304.2	transcription factor Sp5

Pathways from BioSystems

Homologies

Homo sapiens (human)	SP5	NP_001003845.1
Canis lupus familiaris (dog)	SP5	XP_851518.2
Pan troglodytes (chimpanzee)	SP5	XP_001135769.2
Rattus norvegicus (Norway rat)	Sp5	NP_001100022.1
	SP5	XP_001082704.1
Danio rerio (zebrafish)	sp5a	NP_851304.1
Bos taurus (cattle)	SP5	XP_005202450.1
Mus musculus (house mouse)	Sp5	NP_071880.1
Gallus gallus (chicken)	SP5	NP_001038149.1
Xenopus tropicalis (tropical clawed frog)	sp5	NP_989092.1

Gene Ontology
Bibliography

Related articles in PubMed

Lef1-dependent hypothalamic neurogenesis inhibits anxiety.
Xie Y, Kaufmann D, Moulton MJ, Panahi S, Gaynes JA, Watters HN, Zhou D, Xue HH, Fung CM, Levine EM, Letsou A, Brennan KC, Dorsky RI
PLoS biology15(8)e2002257(2017 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry.
Superina S, Borovina A, Ciruna B
Developmental biology387(2)154-66(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

sp5 transcription factors mediate the downstream responses to Wnt/beta-catenin signaling in several developmental processes in zebrafish.
Title: The Sp1-related transcription factors sp5 and sp5-like act downstream of Wnt/beta-catenin signaling in mesoderm and neuroectoderm patterning.

The following sp5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sp5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa40144	NM_180973.3 Latest version!	Danio rerio Sp5 transcription factor a (sp5a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sp5a ( NM_180973.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_851304.2 Danio rerio Sp5 transcription factor a (sp5a), mRNA.

Price & Availability↑

CloneID	ODa40144
Clone ID Related Accession (Same CDS sequence)	NM_180973.3
Accession Version	NM_180973.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Danio rerio(zebrafish)
Product	transcription factor Sp5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR394567.8. On Jun 12, 2015 this sequence version replaced NM_180973.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF388363.1, BC162645.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA3505370 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCTG TGGCTGTGCT GAGGAACGAC ACACTACAGG CGTTTCTGCA GGATCGCACT 
CCGAACTCCT CTCCGGAAAA CAGCAAACAT TCTCCTCTGG CACTTTTAGC AGCGACGTGT 
AACCGGATCG GTCACCACCA CGGATCCACT CCCACGGACT TTATTCAAGT TCCCTACGAC 
ACCACCCTCG GCTCCCCGTC ACGGATTTTC CACCCATGGA GTAATGAGGC CAATCATCAA 
TCCACGCTTT CCAGCAACCC TTCCTTTGGA CTATCCTCTA AATCTCACCT GCAGAGCTCC 
TACGCGTCCC ACCATGAGCT CCCACTGACC CCTCCGGCAG ATCCTACATA TCCGTATGAT 
TTCTCTCCGG TTAAGATGTT GCCATGTTCA ATGCAATCTT TACAGTCCAC CTGCCCGCCG 
ACTTATGTAC CTGCGGTTAC CTACGCAGCA CCTGCCCCCA TCCCTCCAGC CATGCCTAGC 
TTTGTCCCGG GACACTCAGG CCTGGTGCAT CAGCAGCAGA GACAGCTATC CCCAAATCCA 
GGGGAGGATA TACCATGGTG GAGCCTTCAG CAAGGGAATC CTGTAGCCCA TTCGGTGCAC 
CCACACCGCT TCCCCATCCA AAGAGGCTTA GTGTTGGGAC ACACAGATTT CGCACAGTAC 
CAAACTCAAA TCGCAGCCTT GCTGCACACC AAATCTCCGC TGGCTACCGC CCGGCGCTGC 
CGGAGATGCA GATGTCCGAA CTGTCAGTCG TCCAGCTCTA GTGATGAGCC CGGAAAGAAG 
AAACAGCACA TCTGCCACAT CCCCGGATGC GGGAAGGTTT ACGGTAAAAC ATCCCACCTG 
AAGGCGCACC TGCGCTGGCA CTCAGGAGAG AGACCCTTCG TGTGTAACTG GCTCTTCTGC 
GGGAAGAGTT TCACCCGGTC AGATGAGTTA CAGCGGCACC TGCGGACACA CACCGGCGAA 
AAACGCTTTG TGTGTCCGGA CTGCTGTAAA AGGTTCATGA GGAGCGACCA TTTGGCCAAA 
CACGTCAAGA CGCACCAGAA CAAGAAGAGC AAAAGTCACG ACAAAACCTC CGATTATGTG 
AAAAGAGAAG ATCTGAGACC CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851304.2
CDS	145..1251
Translation	MAAVAVLRNDTLQAFLQDRTPNSSPENSKHSPLALLAATCNRIGHHHGSTPTDFIQVPYDTTLGSPSRIFHPWSNEANHQSTLSSNPSFGLSSKSHLQSSYASHHELPLTPPADPTYPYDFSPVKMLPCSMQSLQSTCPPTYVPAVTYAAPAPIPPAMPSFVPGHSGLVHQQQRQLSPNPGEDIPWWSLQQGNPVAHSVHPHRFPIQRGLVLGHTDFAQYQTQIAALLHTKSPLATARRCRRCRCPNCQSSSSSDEPGKKKQHICHIPGCGKVYGKTSHLKAHLRWHSGERPFVCNWLFCGKSFTRSDELQRHLRTHTGEKRFVCPDCCKRFMRSDHLAKHVKTHQNKKSKSHDKTSDYVKREDLRPV

Target ORF information:

RefSeq Version	NM_180973.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Sp5 transcription factor a (sp5a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_180973.3

ORF Insert Sequence:

ATGGCAGCTG TGGCTGTGCT GAGGAACGAC ACACTACAGG CGTTTCTGCA GGATCGCACT 
CCGAACTCCT CTCCGGAAAA CAGCAAACAT TCTCCTCTGG CACTTTTAGC AGCGACGTGT 
AACCGGATCG GTCACCACCA CGGATCCACT CCCACGGACT TTATTCAAGT TCCCTACGAC 
ACCACCCTCG GCTCCCCGTC ACGGATTTTC CACCCATGGA GTAATGAGGC CAATCATCAA 
TCCACGCTTT CCAGCAACCC TTCCTTTGGA CTATCCTCTA AATCTCACCT GCAGAGCTCC 
TACGCGTCCC ACCATGAGCT CCCACTGACC CCTCCGGCAG ATCCTACATA TCCGTATGAT 
TTCTCTCCGG TTAAGATGTT GCCATGTTCA ATGCAATCTT TACAGTCCAC CTGCCCGCCG 
ACTTATGTAC CTGCGGTTAC CTACGCAGCA CCTGCCCCCA TCCCTCCAGC CATGCCTAGC 
TTTGTCCCGG GACACTCAGG CCTGGTGCAT CAGCAGCAGA GACAGCTATC CCCAAATCCA 
GGGGAGGATA TACCATGGTG GAGCCTTCAG CAAGGGAATC CTGTAGCCCA TTCGGTGCAC 
CCACACCGCT TCCCCATCCA AAGAGGCTTA GTGTTGGGAC ACACAGATTT CGCACAGTAC 
CAAACTCAAA TCGCAGCCTT GCTGCACACC AAATCTCCGC TGGCTACCGC CCGGCGCTGC 
CGGAGATGCA GATGTCCGAA CTGTCAGTCG TCCAGCTCTA GTGATGAGCC CGGAAAGAAG 
AAACAGCACA TCTGCCACAT CCCCGGATGC GGGAAGGTTT ACGGTAAAAC ATCCCACCTG 
AAGGCGCACC TGCGCTGGCA CTCAGGAGAG AGACCCTTCG TGTGTAACTG GCTCTTCTGC 
GGGAAGAGTT TCACCCGGTC AGATGAGTTA CAGCGGCACC TGCGGACACA CACCGGCGAA 
AAACGCTTTG TGTGTCCGGA CTGCTGTAAA AGGTTCATGA GGAGCGACCA TTTGGCCAAA 
CACGTCAAGA CGCACCAGAA CAAGAAGAGC AAAAGTCACG ACAAAACCTC CGATTATGTG 
AAAAGAGAAG ATCTGAGACC CGTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lef1-dependent hypothalamic neurogenesis inhibits anxiety.
PLoS biology15(8)e2002257(2017 Aug)
Xie Y,Kaufmann D,Moulton MJ,Panahi S,Gaynes JA,Watters HN,Zhou D,Xue HH,Fung CM,Levine EM,Letsou A,Brennan KC,Dorsky RI

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Analysis of maternal-zygotic ugdh mutants reveals divergent roles for HSPGs in vertebrate embryogenesis and provides new insight into the initiation of left-right asymmetry.
Developmental biology387(2)154-66(2014 Mar)
Superina S,Borovina A,Ciruna B