LOC107596929 cDNA ORF clone, Sinocyclocheilus grahami

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107596929
Entrez Gene ID	107596929
Full Name	membrane-bound transcription factor site-1 protease-like
Gene Type	protein-coding
Organism	Sinocyclocheilus grahami

mRNA and Protein(s)

mRNA	Protein	Name
XM_016287791.1	XP_016143277.1	membrane-bound transcription factor site-1 protease-like isoform X1
XM_016287792.1	XP_016143278.1	membrane-bound transcription factor site-1 protease-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC107596929 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107596929 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi28280	XM_016287792.1 Latest version!	Sinocyclocheilus grahami membrane-bound transcription factor site-1 protease-like (LOC107596929), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSi28280	XM_016287791.1 Latest version!	Sinocyclocheilus grahami membrane-bound transcription factor site-1 protease-like (LOC107596929), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107596929 ( XM_016287791.1 ) cDNA ORF clone, Sinocyclocheilus grahami -> XP_016143277.1 Sinocyclocheilus grahami membrane-bound transcription factor site-1 protease-like (LOC107596929), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSi28280
Clone ID Related Accession (Same CDS sequence)	XM_016287791.1 , XM_016287792.1
Accession Version	XM_016287791.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-18
Organism	Sinocyclocheilus grahami
Product	membrane-bound transcription factor site-1 protease-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015505404.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus grahami Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG ACCAGAAACG GACCGCAGGG ACCATGATTG TACGGCTGTG CTTGCTTGTG 
GCCTTTTTAG GGTGTCGATT GCCTGTGGTG GGCACAGAGG AAGACAGTGG ACCGTTCTCA 
ACACCCTTTC CTTCTCCTCT AAATGCTGAT GCACTCTTAA GCACAAACTG CTCGCAGCTC 
ACACTGAAAC TGGACTTTTC TACCAAAGTG GTCAAGCATG AGTACATTGT TGCATTTAAT 
GGATACTTCA CAGCCAAAGC ACGCAGCGAT TTCATCAGCA GCGCCCTGCG AGATGTGGAT 
GCGGTGAACT GGCGCATCGT ACGGCGGGAC AATCCTGCCA GTGATTACCC CAGTGACTTT 
GAGGTGGTGG AGATCCGGCA GGACACCCGC AGCAGCCTGC TCACTCTGCA GGACCACCCC 
TACATCAAAC GAGTGACGCC CCAGCGCATG GTCCTCCGCA GCCTCAAACT GACAGACCCG 
GGCACTGATG GGGCATCACC TTGCAATGAC ATACGCTGGG TGCAGAAGTG GCAGTCATCA 
AGGCCGCTCA GGAGAACCAG CCTTTCTCTG GGCTCTGGAT TCTGGCATGC TACAGGAAGA 
CACTCCAGTC GCCGTCTCCT ACGGGCCATC CCCAGACATG TTGCCCAGAT ACTGCAAGCA 
GATGTGCTCT GGCAGATGGG ACACACTGGC TCTGGGGTGA AGGTAGCAGT ATTTGACACT 
GGGCTGAGTG AGAAGCACCC TCACTTTAAG AATGTTAAAG AAAGAACCAA CTGGACCAAT 
GAGAAAACAC TGGATGATGG GTTGGGTCAT GGTACCTTTG TTGCTGGGGT TATTGCCAGC 
ATGAGAGAGT GCCAGGGATT CGCTCCTGAT TCTGAGCTTC ATATCTTCAG GGTTTTTACT 
AATAACCAGG TGTCCTACAC CTCTTGGTTC TTAGATGCCT TTAACTATGC CATCCTGAAG 
AAGATAGACG TCTTAAATCT CAGTATAGGG GGACCAGACT TTATGGACCA TCCATTTGTT 
GATAAGGTTT GGGAGCTCAC AGCCAACAAG GTGATTATGG TATCTGCTAT TGGCAATGAT 
GGCCCACTCT ATGGAACTCT GAACAATCCA GCAGATCAGA TGGATGTTAT TGGTGTTGGA 
GGGATTGATT TTGAGGACAA CATTGCCAGA TTCTCATCCC GAGGCATGAC AACATGGGAG 
CTGCCAGGTG GCTATGGCAG GGTGAAGCCT GACATTGTGA CATACGGCTC TGGTGTGCGT 
GGCTCAGGAT TGAAGGAGGG CTGTCGTTCT CTCTCTGGCA CCAGTGTTGC ATCACCTGTA 
GTGGCCGGGG CTGTCACACT ACTGGCCAGC ACTGTGTTGA ACCGAGAGTT GGTGAACCCG 
GCCAGTATGA AGCAGGCCCT GATCGCCTCA GCTCGCAGGC TGCCTGGCGT CAACATGTTT 
GAGCAGGGCC ATGGCAAACT TGATTTACTC CGAGCCTACC AGATCCTTAA CAGCTACAAG 
CCACAAGCCA GCCTGAGTCC GAGCTACATA GATCTGACCG AGTGCCCCTA TATGTGGCCC 
TACTGCTCTC AGCCTATTTA CTACGGTGGC ATGCCGACGA TTGTCAATGT GACCATTCTT 
AATGGCATGG GTGTGACTGG AAGGATTGTG GACAAGCCCA TCTGGCAACC CTACCTTCCT 
CAGAATGGGG ATTATGTGGA CGTGGCGGTG TCTTACTCGC CTGTACTCTG GCCCTGGGCC 
GGATACCTTG CTATTTCCGT ATCTGTGGCC AAGAAAGCTG CATCGTGGGA AGGCATCGCT 
CAGGGTCATG TCATGGTTAC AGTGGCCTCA CCAGCAGAGA ATGATTCAGC AATAGCTGGT 
GAGATGACCT CTACAGTGAA GTTACCAGTG AAGGTAAAGA TTGTCCCCAC TCCTCCACGT 
AGTAAGAGGA TTCTGTGGGA TCAGTACCAC AACCTACGCT ACCCACCTGG CTACTTCCCT 
CGAGACAACC TGCGCATGAA GAACGATCCG CTTGACTGGA ATGGTGATCA TATTCACACA 
AACTTTAGAG ACATGTATCA GCACCTTCGC AGCATGGGCT ACTTTGTCGA AGTCTTGGGT 
GCACCAATCA CATGCTTTGA TGCCAGTCAG TATGGGACCT TGCTGATGGT GGACAGTGAG 
GAAGAGTATT TTCCAGAGGA AATCACCAAG TTGAGGAGAG ACATAGATAA CGGCCTGTCT 
CTTATCATCT TTAGTGACTG GTACAATACA TCAGTCATGA GAAAAGTGAA GTTCTATGAT 
GAGAACACCA GGCAATGGTG GATGCCGGAC ACAGGGGGCG CTAATGTGCC AGCCTTGAAT 
GACCTGATTG CAGTGTGGGG TATGGCCTTC AGTGACGGTC TGTATGAGGG AGACTTCACA 
ATGGCAGATC ATGACATGTA TTATGCCTCG GGGTGCAGCA TCGCTCGTTT TCCTGAGGAT 
GGGATTGTCG TCGCTCAGAC CTTAAAGGAT CAAGGTCTGG AGGTCTTGAA ACAAGAGACT 
GCAGTGGTGG AAAATGTGCC TGTGCTTGGA CTGTACCAAA CACCCTCTGA GGGGGGAGGC 
CGTATAGCAC TCTACGGAGA CTCCAACTGC ATTGATGACA GTCATAGGCA GAAAGACTGT 
TTTTGGCTTC TGGATGCTCT GCTACAGTAC ACTTCCTATA GCATGACTCC ACCCAGCCTG 
ACCCACTCTA AAAACAGAGT AGTGCCCCCT ACAGGCACCG ACAGGCCATT ACCACAGAGA 
TTGGAAGGTA ATCACCTCTA CCGTTACTCC AAAGTGCTGG AGGCTCATTT GGGTGACCCT 
AAGCCTCGTC CTCTACCTGC ATGTCCTCAT CTGTCTTGGG CTAAACCCCA GCCGCTTAAT 
GAGACCGCTC CTAGCAATCT GTGGAAGCAC CAGAAGCTGC TGTCAGTGGA CATGGACAAG 
GTTGTTTTGC CTAATGTGAG GCTATATCGG CCTCAGGTTC GACCCCTGTC TCCTGGAGAA 
AGTGGAGCCT GGGACATCCC TGGAGGAATA ATGCCCGGCC GCTATAATCA AGAGGTGGGC 
CAAACCATCC CAATGTTTGC CTTTCTTGGT GCCATGGTTG TTCTTTCCTT CTTTGTGGTG 
CAATTGACCA AGGCCAAGAG CAAGCCCAAG CGTAGGAAAC CCAGAGTGAA ACGACCACTT 
TACCTTCAAC AACAGCAACA ACAAACCCCT CACTCAGGCA AAAACCCCAC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016143277.1
CDS	300..3536
Translation	MGNDQKRTAGTMIVRLCLLVAFLGCRLPVVGTEEDSGPFSTPFPSPLNADALLSTNCSQLTLKLDFSTKVVKHEYIVAFNGYFTAKARSDFISSALRDVDAVNWRIVRRDNPASDYPSDFEVVEIRQDTRSSLLTLQDHPYIKRVTPQRMVLRSLKLTDPGTDGASPCNDIRWVQKWQSSRPLRRTSLSLGSGFWHATGRHSSRRLLRAIPRHVAQILQADVLWQMGHTGSGVKVAVFDTGLSEKHPHFKNVKERTNWTNEKTLDDGLGHGTFVAGVIASMRECQGFAPDSELHIFRVFTNNQVSYTSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANKVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGSGVRGSGLKEGCRSLSGTSVASPVVAGAVTLLASTVLNRELVNPASMKQALIASARRLPGVNMFEQGHGKLDLLRAYQILNSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPIWQPYLPQNGDYVDVAVSYSPVLWPWAGYLAISVSVAKKAASWEGIAQGHVMVTVASPAENDSAIAGEMTSTVKLPVKVKIVPTPPRSKRILWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHIHTNFRDMYQHLRSMGYFVEVLGAPITCFDASQYGTLLMVDSEEEYFPEEITKLRRDIDNGLSLIIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNDLIAVWGMAFSDGLYEGDFTMADHDMYYASGCSIARFPEDGIVVAQTLKDQGLEVLKQETAVVENVPVLGLYQTPSEGGGRIALYGDSNCIDDSHRQKDCFWLLDALLQYTSYSMTPPSLTHSKNRVVPPTGTDRPLPQRLEGNHLYRYSKVLEAHLGDPKPRPLPACPHLSWAKPQPLNETAPSNLWKHQKLLSVDMDKVVLPNVRLYRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPMFAFLGAMVVLSFFVVQLTKAKSKPKRRKPRVKRPLYLQQQQQQTPHSGKNPTV

Target ORF information:

RefSeq Version	XM_016287791.1
Organism	Sinocyclocheilus grahami
Definition	Sinocyclocheilus grahami membrane-bound transcription factor site-1 protease-like (LOC107596929), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016287791.1

ORF Insert Sequence:

ATGGGCAATG ACCAGAAACG GACCGCAGGG ACCATGATTG TACGGCTGTG CTTGCTTGTG 
GCCTTTTTAG GGTGTCGATT GCCTGTGGTG GGCACAGAGG AAGACAGTGG ACCGTTCTCA 
ACACCCTTTC CTTCTCCTCT AAATGCTGAT GCACTCTTAA GCACAAACTG CTCGCAGCTC 
ACACTGAAAC TGGACTTTTC TACCAAAGTG GTCAAGCATG AGTACATTGT TGCATTTAAT 
GGATACTTCA CAGCCAAAGC ACGCAGCGAT TTCATCAGCA GCGCCCTGCG AGATGTGGAT 
GCGGTGAACT GGCGCATCGT ACGGCGGGAC AATCCTGCCA GTGATTACCC CAGTGACTTT 
GAGGTGGTGG AGATCCGGCA GGACACCCGC AGCAGCCTGC TCACTCTGCA GGACCACCCC 
TACATCAAAC GAGTGACGCC CCAGCGCATG GTCCTCCGCA GCCTCAAACT GACAGACCCG 
GGCACTGATG GGGCATCACC TTGCAATGAC ATACGCTGGG TGCAGAAGTG GCAGTCATCA 
AGGCCGCTCA GGAGAACCAG CCTTTCTCTG GGCTCTGGAT TCTGGCATGC TACAGGAAGA 
CACTCCAGTC GCCGTCTCCT ACGGGCCATC CCCAGACATG TTGCCCAGAT ACTGCAAGCA 
GATGTGCTCT GGCAGATGGG ACACACTGGC TCTGGGGTGA AGGTAGCAGT ATTTGACACT 
GGGCTGAGTG AGAAGCACCC TCACTTTAAG AATGTTAAAG AAAGAACCAA CTGGACCAAT 
GAGAAAACAC TGGATGATGG GTTGGGTCAT GGTACCTTTG TTGCTGGGGT TATTGCCAGC 
ATGAGAGAGT GCCAGGGATT CGCTCCTGAT TCTGAGCTTC ATATCTTCAG GGTTTTTACT 
AATAACCAGG TGTCCTACAC CTCTTGGTTC TTAGATGCCT TTAACTATGC CATCCTGAAG 
AAGATAGACG TCTTAAATCT CAGTATAGGG GGACCAGACT TTATGGACCA TCCATTTGTT 
GATAAGGTTT GGGAGCTCAC AGCCAACAAG GTGATTATGG TATCTGCTAT TGGCAATGAT 
GGCCCACTCT ATGGAACTCT GAACAATCCA GCAGATCAGA TGGATGTTAT TGGTGTTGGA 
GGGATTGATT TTGAGGACAA CATTGCCAGA TTCTCATCCC GAGGCATGAC AACATGGGAG 
CTGCCAGGTG GCTATGGCAG GGTGAAGCCT GACATTGTGA CATACGGCTC TGGTGTGCGT 
GGCTCAGGAT TGAAGGAGGG CTGTCGTTCT CTCTCTGGCA CCAGTGTTGC ATCACCTGTA 
GTGGCCGGGG CTGTCACACT ACTGGCCAGC ACTGTGTTGA ACCGAGAGTT GGTGAACCCG 
GCCAGTATGA AGCAGGCCCT GATCGCCTCA GCTCGCAGGC TGCCTGGCGT CAACATGTTT 
GAGCAGGGCC ATGGCAAACT TGATTTACTC CGAGCCTACC AGATCCTTAA CAGCTACAAG 
CCACAAGCCA GCCTGAGTCC GAGCTACATA GATCTGACCG AGTGCCCCTA TATGTGGCCC 
TACTGCTCTC AGCCTATTTA CTACGGTGGC ATGCCGACGA TTGTCAATGT GACCATTCTT 
AATGGCATGG GTGTGACTGG AAGGATTGTG GACAAGCCCA TCTGGCAACC CTACCTTCCT 
CAGAATGGGG ATTATGTGGA CGTGGCGGTG TCTTACTCGC CTGTACTCTG GCCCTGGGCC 
GGATACCTTG CTATTTCCGT ATCTGTGGCC AAGAAAGCTG CATCGTGGGA AGGCATCGCT 
CAGGGTCATG TCATGGTTAC AGTGGCCTCA CCAGCAGAGA ATGATTCAGC AATAGCTGGT 
GAGATGACCT CTACAGTGAA GTTACCAGTG AAGGTAAAGA TTGTCCCCAC TCCTCCACGT 
AGTAAGAGGA TTCTGTGGGA TCAGTACCAC AACCTACGCT ACCCACCTGG CTACTTCCCT 
CGAGACAACC TGCGCATGAA GAACGATCCG CTTGACTGGA ATGGTGATCA TATTCACACA 
AACTTTAGAG ACATGTATCA GCACCTTCGC AGCATGGGCT ACTTTGTCGA AGTCTTGGGT 
GCACCAATCA CATGCTTTGA TGCCAGTCAG TATGGGACCT TGCTGATGGT GGACAGTGAG 
GAAGAGTATT TTCCAGAGGA AATCACCAAG TTGAGGAGAG ACATAGATAA CGGCCTGTCT 
CTTATCATCT TTAGTGACTG GTACAATACA TCAGTCATGA GAAAAGTGAA GTTCTATGAT 
GAGAACACCA GGCAATGGTG GATGCCGGAC ACAGGGGGCG CTAATGTGCC AGCCTTGAAT 
GACCTGATTG CAGTGTGGGG TATGGCCTTC AGTGACGGTC TGTATGAGGG AGACTTCACA 
ATGGCAGATC ATGACATGTA TTATGCCTCG GGGTGCAGCA TCGCTCGTTT TCCTGAGGAT 
GGGATTGTCG TCGCTCAGAC CTTAAAGGAT CAAGGTCTGG AGGTCTTGAA ACAAGAGACT 
GCAGTGGTGG AAAATGTGCC TGTGCTTGGA CTGTACCAAA CACCCTCTGA GGGGGGAGGC 
CGTATAGCAC TCTACGGAGA CTCCAACTGC ATTGATGACA GTCATAGGCA GAAAGACTGT 
TTTTGGCTTC TGGATGCTCT GCTACAGTAC ACTTCCTATA GCATGACTCC ACCCAGCCTG 
ACCCACTCTA AAAACAGAGT AGTGCCCCCT ACAGGCACCG ACAGGCCATT ACCACAGAGA 
TTGGAAGGTA ATCACCTCTA CCGTTACTCC AAAGTGCTGG AGGCTCATTT GGGTGACCCT 
AAGCCTCGTC CTCTACCTGC ATGTCCTCAT CTGTCTTGGG CTAAACCCCA GCCGCTTAAT 
GAGACCGCTC CTAGCAATCT GTGGAAGCAC CAGAAGCTGC TGTCAGTGGA CATGGACAAG 
GTTGTTTTGC CTAATGTGAG GCTATATCGG CCTCAGGTTC GACCCCTGTC TCCTGGAGAA 
AGTGGAGCCT GGGACATCCC TGGAGGAATA ATGCCCGGCC GCTATAATCA AGAGGTGGGC 
CAAACCATCC CAATGTTTGC CTTTCTTGGT GCCATGGTTG TTCTTTCCTT CTTTGTGGTG 
CAATTGACCA AGGCCAAGAG CAAGCCCAAG CGTAGGAAAC CCAGAGTGAA ACGACCACTT 
TACCTTCAAC AACAGCAACA ACAAACCCCT CACTCAGGCA AAAACCCCAC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107596929 ( XM_016287792.1 ) cDNA ORF clone, Sinocyclocheilus grahami -> XP_016143278.1 Sinocyclocheilus grahami membrane-bound transcription factor site-1 protease-like (LOC107596929), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSi28280
Clone ID Related Accession (Same CDS sequence)	XM_016287791.1 , XM_016287792.1
Accession Version	XM_016287792.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-18
Organism	Sinocyclocheilus grahami
Product	membrane-bound transcription factor site-1 protease-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015505404.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus grahami Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCAATG ACCAGAAACG GACCGCAGGG ACCATGATTG TACGGCTGTG CTTGCTTGTG 
GCCTTTTTAG GGTGTCGATT GCCTGTGGTG GGCACAGAGG AAGACAGTGG ACCGTTCTCA 
ACACCCTTTC CTTCTCCTCT AAATGCTGAT GCACTCTTAA GCACAAACTG CTCGCAGCTC 
ACACTGAAAC TGGACTTTTC TACCAAAGTG GTCAAGCATG AGTACATTGT TGCATTTAAT 
GGATACTTCA CAGCCAAAGC ACGCAGCGAT TTCATCAGCA GCGCCCTGCG AGATGTGGAT 
GCGGTGAACT GGCGCATCGT ACGGCGGGAC AATCCTGCCA GTGATTACCC CAGTGACTTT 
GAGGTGGTGG AGATCCGGCA GGACACCCGC AGCAGCCTGC TCACTCTGCA GGACCACCCC 
TACATCAAAC GAGTGACGCC CCAGCGCATG GTCCTCCGCA GCCTCAAACT GACAGACCCG 
GGCACTGATG GGGCATCACC TTGCAATGAC ATACGCTGGG TGCAGAAGTG GCAGTCATCA 
AGGCCGCTCA GGAGAACCAG CCTTTCTCTG GGCTCTGGAT TCTGGCATGC TACAGGAAGA 
CACTCCAGTC GCCGTCTCCT ACGGGCCATC CCCAGACATG TTGCCCAGAT ACTGCAAGCA 
GATGTGCTCT GGCAGATGGG ACACACTGGC TCTGGGGTGA AGGTAGCAGT ATTTGACACT 
GGGCTGAGTG AGAAGCACCC TCACTTTAAG AATGTTAAAG AAAGAACCAA CTGGACCAAT 
GAGAAAACAC TGGATGATGG GTTGGGTCAT GGTACCTTTG TTGCTGGGGT TATTGCCAGC 
ATGAGAGAGT GCCAGGGATT CGCTCCTGAT TCTGAGCTTC ATATCTTCAG GGTTTTTACT 
AATAACCAGG TGTCCTACAC CTCTTGGTTC TTAGATGCCT TTAACTATGC CATCCTGAAG 
AAGATAGACG TCTTAAATCT CAGTATAGGG GGACCAGACT TTATGGACCA TCCATTTGTT 
GATAAGGTTT GGGAGCTCAC AGCCAACAAG GTGATTATGG TATCTGCTAT TGGCAATGAT 
GGCCCACTCT ATGGAACTCT GAACAATCCA GCAGATCAGA TGGATGTTAT TGGTGTTGGA 
GGGATTGATT TTGAGGACAA CATTGCCAGA TTCTCATCCC GAGGCATGAC AACATGGGAG 
CTGCCAGGTG GCTATGGCAG GGTGAAGCCT GACATTGTGA CATACGGCTC TGGTGTGCGT 
GGCTCAGGAT TGAAGGAGGG CTGTCGTTCT CTCTCTGGCA CCAGTGTTGC ATCACCTGTA 
GTGGCCGGGG CTGTCACACT ACTGGCCAGC ACTGTGTTGA ACCGAGAGTT GGTGAACCCG 
GCCAGTATGA AGCAGGCCCT GATCGCCTCA GCTCGCAGGC TGCCTGGCGT CAACATGTTT 
GAGCAGGGCC ATGGCAAACT TGATTTACTC CGAGCCTACC AGATCCTTAA CAGCTACAAG 
CCACAAGCCA GCCTGAGTCC GAGCTACATA GATCTGACCG AGTGCCCCTA TATGTGGCCC 
TACTGCTCTC AGCCTATTTA CTACGGTGGC ATGCCGACGA TTGTCAATGT GACCATTCTT 
AATGGCATGG GTGTGACTGG AAGGATTGTG GACAAGCCCA TCTGGCAACC CTACCTTCCT 
CAGAATGGGG ATTATGTGGA CGTGGCGGTG TCTTACTCGC CTGTACTCTG GCCCTGGGCC 
GGATACCTTG CTATTTCCGT ATCTGTGGCC AAGAAAGCTG CATCGTGGGA AGGCATCGCT 
CAGGGTCATG TCATGGTTAC AGTGGCCTCA CCAGCAGAGA ATGATTCAGC AATAGCTGGT 
GAGATGACCT CTACAGTGAA GTTACCAGTG AAGGTAAAGA TTGTCCCCAC TCCTCCACGT 
AGTAAGAGGA TTCTGTGGGA TCAGTACCAC AACCTACGCT ACCCACCTGG CTACTTCCCT 
CGAGACAACC TGCGCATGAA GAACGATCCG CTTGACTGGA ATGGTGATCA TATTCACACA 
AACTTTAGAG ACATGTATCA GCACCTTCGC AGCATGGGCT ACTTTGTCGA AGTCTTGGGT 
GCACCAATCA CATGCTTTGA TGCCAGTCAG TATGGGACCT TGCTGATGGT GGACAGTGAG 
GAAGAGTATT TTCCAGAGGA AATCACCAAG TTGAGGAGAG ACATAGATAA CGGCCTGTCT 
CTTATCATCT TTAGTGACTG GTACAATACA TCAGTCATGA GAAAAGTGAA GTTCTATGAT 
GAGAACACCA GGCAATGGTG GATGCCGGAC ACAGGGGGCG CTAATGTGCC AGCCTTGAAT 
GACCTGATTG CAGTGTGGGG TATGGCCTTC AGTGACGGTC TGTATGAGGG AGACTTCACA 
ATGGCAGATC ATGACATGTA TTATGCCTCG GGGTGCAGCA TCGCTCGTTT TCCTGAGGAT 
GGGATTGTCG TCGCTCAGAC CTTAAAGGAT CAAGGTCTGG AGGTCTTGAA ACAAGAGACT 
GCAGTGGTGG AAAATGTGCC TGTGCTTGGA CTGTACCAAA CACCCTCTGA GGGGGGAGGC 
CGTATAGCAC TCTACGGAGA CTCCAACTGC ATTGATGACA GTCATAGGCA GAAAGACTGT 
TTTTGGCTTC TGGATGCTCT GCTACAGTAC ACTTCCTATA GCATGACTCC ACCCAGCCTG 
ACCCACTCTA AAAACAGAGT AGTGCCCCCT ACAGGCACCG ACAGGCCATT ACCACAGAGA 
TTGGAAGGTA ATCACCTCTA CCGTTACTCC AAAGTGCTGG AGGCTCATTT GGGTGACCCT 
AAGCCTCGTC CTCTACCTGC ATGTCCTCAT CTGTCTTGGG CTAAACCCCA GCCGCTTAAT 
GAGACCGCTC CTAGCAATCT GTGGAAGCAC CAGAAGCTGC TGTCAGTGGA CATGGACAAG 
GTTGTTTTGC CTAATGTGAG GCTATATCGG CCTCAGGTTC GACCCCTGTC TCCTGGAGAA 
AGTGGAGCCT GGGACATCCC TGGAGGAATA ATGCCCGGCC GCTATAATCA AGAGGTGGGC 
CAAACCATCC CAATGTTTGC CTTTCTTGGT GCCATGGTTG TTCTTTCCTT CTTTGTGGTG 
CAATTGACCA AGGCCAAGAG CAAGCCCAAG CGTAGGAAAC CCAGAGTGAA ACGACCACTT 
TACCTTCAAC AACAGCAACA ACAAACCCCT CACTCAGGCA AAAACCCCAC CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016143278.1
CDS	461..3697
Translation	MGNDQKRTAGTMIVRLCLLVAFLGCRLPVVGTEEDSGPFSTPFPSPLNADALLSTNCSQLTLKLDFSTKVVKHEYIVAFNGYFTAKARSDFISSALRDVDAVNWRIVRRDNPASDYPSDFEVVEIRQDTRSSLLTLQDHPYIKRVTPQRMVLRSLKLTDPGTDGASPCNDIRWVQKWQSSRPLRRTSLSLGSGFWHATGRHSSRRLLRAIPRHVAQILQADVLWQMGHTGSGVKVAVFDTGLSEKHPHFKNVKERTNWTNEKTLDDGLGHGTFVAGVIASMRECQGFAPDSELHIFRVFTNNQVSYTSWFLDAFNYAILKKIDVLNLSIGGPDFMDHPFVDKVWELTANKVIMVSAIGNDGPLYGTLNNPADQMDVIGVGGIDFEDNIARFSSRGMTTWELPGGYGRVKPDIVTYGSGVRGSGLKEGCRSLSGTSVASPVVAGAVTLLASTVLNRELVNPASMKQALIASARRLPGVNMFEQGHGKLDLLRAYQILNSYKPQASLSPSYIDLTECPYMWPYCSQPIYYGGMPTIVNVTILNGMGVTGRIVDKPIWQPYLPQNGDYVDVAVSYSPVLWPWAGYLAISVSVAKKAASWEGIAQGHVMVTVASPAENDSAIAGEMTSTVKLPVKVKIVPTPPRSKRILWDQYHNLRYPPGYFPRDNLRMKNDPLDWNGDHIHTNFRDMYQHLRSMGYFVEVLGAPITCFDASQYGTLLMVDSEEEYFPEEITKLRRDIDNGLSLIIFSDWYNTSVMRKVKFYDENTRQWWMPDTGGANVPALNDLIAVWGMAFSDGLYEGDFTMADHDMYYASGCSIARFPEDGIVVAQTLKDQGLEVLKQETAVVENVPVLGLYQTPSEGGGRIALYGDSNCIDDSHRQKDCFWLLDALLQYTSYSMTPPSLTHSKNRVVPPTGTDRPLPQRLEGNHLYRYSKVLEAHLGDPKPRPLPACPHLSWAKPQPLNETAPSNLWKHQKLLSVDMDKVVLPNVRLYRPQVRPLSPGESGAWDIPGGIMPGRYNQEVGQTIPMFAFLGAMVVLSFFVVQLTKAKSKPKRRKPRVKRPLYLQQQQQQTPHSGKNPTV

Target ORF information:

RefSeq Version	XM_016287792.1
Organism	Sinocyclocheilus grahami
Definition	Sinocyclocheilus grahami membrane-bound transcription factor site-1 protease-like (LOC107596929), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016287792.1

ORF Insert Sequence:

ATGGGCAATG ACCAGAAACG GACCGCAGGG ACCATGATTG TACGGCTGTG CTTGCTTGTG 
GCCTTTTTAG GGTGTCGATT GCCTGTGGTG GGCACAGAGG AAGACAGTGG ACCGTTCTCA 
ACACCCTTTC CTTCTCCTCT AAATGCTGAT GCACTCTTAA GCACAAACTG CTCGCAGCTC 
ACACTGAAAC TGGACTTTTC TACCAAAGTG GTCAAGCATG AGTACATTGT TGCATTTAAT 
GGATACTTCA CAGCCAAAGC ACGCAGCGAT TTCATCAGCA GCGCCCTGCG AGATGTGGAT 
GCGGTGAACT GGCGCATCGT ACGGCGGGAC AATCCTGCCA GTGATTACCC CAGTGACTTT 
GAGGTGGTGG AGATCCGGCA GGACACCCGC AGCAGCCTGC TCACTCTGCA GGACCACCCC 
TACATCAAAC GAGTGACGCC CCAGCGCATG GTCCTCCGCA GCCTCAAACT GACAGACCCG 
GGCACTGATG GGGCATCACC TTGCAATGAC ATACGCTGGG TGCAGAAGTG GCAGTCATCA 
AGGCCGCTCA GGAGAACCAG CCTTTCTCTG GGCTCTGGAT TCTGGCATGC TACAGGAAGA 
CACTCCAGTC GCCGTCTCCT ACGGGCCATC CCCAGACATG TTGCCCAGAT ACTGCAAGCA 
GATGTGCTCT GGCAGATGGG ACACACTGGC TCTGGGGTGA AGGTAGCAGT ATTTGACACT 
GGGCTGAGTG AGAAGCACCC TCACTTTAAG AATGTTAAAG AAAGAACCAA CTGGACCAAT 
GAGAAAACAC TGGATGATGG GTTGGGTCAT GGTACCTTTG TTGCTGGGGT TATTGCCAGC 
ATGAGAGAGT GCCAGGGATT CGCTCCTGAT TCTGAGCTTC ATATCTTCAG GGTTTTTACT 
AATAACCAGG TGTCCTACAC CTCTTGGTTC TTAGATGCCT TTAACTATGC CATCCTGAAG 
AAGATAGACG TCTTAAATCT CAGTATAGGG GGACCAGACT TTATGGACCA TCCATTTGTT 
GATAAGGTTT GGGAGCTCAC AGCCAACAAG GTGATTATGG TATCTGCTAT TGGCAATGAT 
GGCCCACTCT ATGGAACTCT GAACAATCCA GCAGATCAGA TGGATGTTAT TGGTGTTGGA 
GGGATTGATT TTGAGGACAA CATTGCCAGA TTCTCATCCC GAGGCATGAC AACATGGGAG 
CTGCCAGGTG GCTATGGCAG GGTGAAGCCT GACATTGTGA CATACGGCTC TGGTGTGCGT 
GGCTCAGGAT TGAAGGAGGG CTGTCGTTCT CTCTCTGGCA CCAGTGTTGC ATCACCTGTA 
GTGGCCGGGG CTGTCACACT ACTGGCCAGC ACTGTGTTGA ACCGAGAGTT GGTGAACCCG 
GCCAGTATGA AGCAGGCCCT GATCGCCTCA GCTCGCAGGC TGCCTGGCGT CAACATGTTT 
GAGCAGGGCC ATGGCAAACT TGATTTACTC CGAGCCTACC AGATCCTTAA CAGCTACAAG 
CCACAAGCCA GCCTGAGTCC GAGCTACATA GATCTGACCG AGTGCCCCTA TATGTGGCCC 
TACTGCTCTC AGCCTATTTA CTACGGTGGC ATGCCGACGA TTGTCAATGT GACCATTCTT 
AATGGCATGG GTGTGACTGG AAGGATTGTG GACAAGCCCA TCTGGCAACC CTACCTTCCT 
CAGAATGGGG ATTATGTGGA CGTGGCGGTG TCTTACTCGC CTGTACTCTG GCCCTGGGCC 
GGATACCTTG CTATTTCCGT ATCTGTGGCC AAGAAAGCTG CATCGTGGGA AGGCATCGCT 
CAGGGTCATG TCATGGTTAC AGTGGCCTCA CCAGCAGAGA ATGATTCAGC AATAGCTGGT 
GAGATGACCT CTACAGTGAA GTTACCAGTG AAGGTAAAGA TTGTCCCCAC TCCTCCACGT 
AGTAAGAGGA TTCTGTGGGA TCAGTACCAC AACCTACGCT ACCCACCTGG CTACTTCCCT 
CGAGACAACC TGCGCATGAA GAACGATCCG CTTGACTGGA ATGGTGATCA TATTCACACA 
AACTTTAGAG ACATGTATCA GCACCTTCGC AGCATGGGCT ACTTTGTCGA AGTCTTGGGT 
GCACCAATCA CATGCTTTGA TGCCAGTCAG TATGGGACCT TGCTGATGGT GGACAGTGAG 
GAAGAGTATT TTCCAGAGGA AATCACCAAG TTGAGGAGAG ACATAGATAA CGGCCTGTCT 
CTTATCATCT TTAGTGACTG GTACAATACA TCAGTCATGA GAAAAGTGAA GTTCTATGAT 
GAGAACACCA GGCAATGGTG GATGCCGGAC ACAGGGGGCG CTAATGTGCC AGCCTTGAAT 
GACCTGATTG CAGTGTGGGG TATGGCCTTC AGTGACGGTC TGTATGAGGG AGACTTCACA 
ATGGCAGATC ATGACATGTA TTATGCCTCG GGGTGCAGCA TCGCTCGTTT TCCTGAGGAT 
GGGATTGTCG TCGCTCAGAC CTTAAAGGAT CAAGGTCTGG AGGTCTTGAA ACAAGAGACT 
GCAGTGGTGG AAAATGTGCC TGTGCTTGGA CTGTACCAAA CACCCTCTGA GGGGGGAGGC 
CGTATAGCAC TCTACGGAGA CTCCAACTGC ATTGATGACA GTCATAGGCA GAAAGACTGT 
TTTTGGCTTC TGGATGCTCT GCTACAGTAC ACTTCCTATA GCATGACTCC ACCCAGCCTG 
ACCCACTCTA AAAACAGAGT AGTGCCCCCT ACAGGCACCG ACAGGCCATT ACCACAGAGA 
TTGGAAGGTA ATCACCTCTA CCGTTACTCC AAAGTGCTGG AGGCTCATTT GGGTGACCCT 
AAGCCTCGTC CTCTACCTGC ATGTCCTCAT CTGTCTTGGG CTAAACCCCA GCCGCTTAAT 
GAGACCGCTC CTAGCAATCT GTGGAAGCAC CAGAAGCTGC TGTCAGTGGA CATGGACAAG 
GTTGTTTTGC CTAATGTGAG GCTATATCGG CCTCAGGTTC GACCCCTGTC TCCTGGAGAA 
AGTGGAGCCT GGGACATCCC TGGAGGAATA ATGCCCGGCC GCTATAATCA AGAGGTGGGC 
CAAACCATCC CAATGTTTGC CTTTCTTGGT GCCATGGTTG TTCTTTCCTT CTTTGTGGTG 
CAATTGACCA AGGCCAAGAG CAAGCCCAAG CGTAGGAAAC CCAGAGTGAA ACGACCACTT 
TACCTTCAAC AACAGCAACA ACAAACCCCT CACTCAGGCA AAAACCCCAC CGTATGA