Smg5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smg5
Entrez Gene ID	681012
Full Name	SMG5 nonsense mediated mRNA decay factor
General protein information	Preferred Names SMG5 nonsense mediated mRNA decay factor Names protein SMG5 Smg-5 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_001059906.6	XP_001059906.3	protein SMG5
XM_002725989.5	XP_002726035.1	protein SMG5

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMG5	XP_513880.3
	SMG5	XP_002801899.1
Canis lupus familiaris (dog)	SMG5	XP_547535.3
Bos taurus (cattle)	SMG5	NP_001077148.1
Rattus norvegicus (Norway rat)	Smg5	XP_001059906.3
Gallus gallus (chicken)	SMG5	XP_423228.4
Xenopus tropicalis (tropical clawed frog)	smg5	NP_001072622.1
Homo sapiens (human)	SMG5	NP_056142.2
Mus musculus (house mouse)	Smg5	NP_839977.2

Gene Ontology

The following Smg5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smg5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa15283	XM_001059906.6 Latest version!	Rattus norvegicus SMG5 nonsense mediated mRNA decay factor (Smg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa15283	XM_002725989.5 Latest version!	Rattus norvegicus SMG5 nonsense mediated mRNA decay factor (Smg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smg5 ( XM_001059906.6 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001059906.3 Rattus norvegicus SMG5 nonsense mediated mRNA decay factor (Smg5), mRNA.

Price & Availability↑

CloneID	ORa15283
Clone ID Related Accession (Same CDS sequence)	XM_001059906.6 , XM_002725989.5
Accession Version	XM_001059906.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001059906.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCTCCTCC AGTGGAGAGC AGCGAACCTG AAGCAAAGGT CCTCCACACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGGC TAGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAGGAAGT GTTTAAACCA GAGAACGTTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGTG TGAAGCTTAT GTTCTTGCAC CCAGTAGACT ATGGGAGGAA GGCTGAGGAA 
CTGCTGTGGA GAAAGGTATA CTATGAAGTC ATCCAACTCA TCAAGACTAA CAAAAAGCAC 
ATCCACAGTC GGAGCACCTT GGAATGTGCC TACAGGACTC ACCTGGTCGC CGGCATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAA TCCCACTACC AGCTGGAACT ACAGTGCTGC 
ATTGACTGGA CTCATGTCAC TGATCCCCTC ATAGGATGTA AGAAGCCAGT ATCTGCTTCA 
GGAAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
CTGTCACGCT ATCAGAATGA ACTGGCTGGT GTGGACACTG AGCTGCTAGC TGAGAGATTC 
TACTATCAAG CCTTGTCAGT CGCGCCCCAG ATTGGAATGC CCTTCAACCA GCTGGGCACT 
CTTGCAGGCA GCAAGTATTA CAGTGTGGAA GCCATGTACT GTTACTTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGA GACTCTATGA CAAGGCAGCA 
AAAATGTACC ACCAGTTGAA GAAGTCTGAG ACCAGGAAGC TCTCCCCTAG CAAAAAGCGA 
TGCAAAGACA TTAAGAGGTT GTTGGTGAAC TTCATGTACC TACAAAGCCT GTTACAGCCC 
AAAAGTAGCT CTGTGGACTC AGAACTGACC TCACTTTGCC AGTCAGTCCT AGAGGATTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAGCCTTG GTCTGACGAA CGAGGATGAG 
GAGGAGTGTG AGAGTGGATA TGCTTTCCTG CCCGACCTGC TCATCTTTCA GATGGCCATC 
ATCTGCCTTA TGGGTGTGCA CAGTTTAAAA AGAGCAGGAT CCAAGCAGTA TAGTGCAGCC 
ATTGCTTTCA CCCTGGCCCT CTTCTCCCAC CTTATTAATC ATGTCAACAT ACGACTGCAG 
GCAGAGCTGG AAGAGGGCGA GAACCCTGTC TCAGCTTTTC AGAGTGATGG CACAGATGAA 
CCAGAGTCAA AAGAAGCACT AGAAAAAGAG GAGCCAGAGC CAGAGCCTCC CACTGTGGTG 
CCCCAAGCCG ATGAGGGTAG AAAGAGTCGT AAGCACTCCC GACTCTCTTG TCTTCGTCGC 
CGCCGCCGCC ACCACCCTCC TAAGGCTGGT GATGACAGTG ACCTGAGTGA GGGTTTTGAG 
TCAGACTCTA GCCATGACTC TGCCCAGGCC AGTGATGGAT CAGACAGTGG CTCTGACAAG 
AGCCTGGAAG GCAGGGGCAC TGCTTTTGAT GCAGAAACAG ACTCAGAGAT GAACAGCCAG 
GAGTCCCGGT CAGACCTGGA AGATATAGAG GATGAAGAGG GGACACGGTC TCCAGCCCAG 
GAGCCCCCTC AGACCAGATC AGAGGTTCCA GATTCCCTCA ATGGCCCCTT GGGCCCTAGT 
GAGGCAAGCA TTGCCAGCAA TTTACAAGCC ATGTCCACCC AGATGTTCCA GACCAAGCGC 
TGCTTCCGAC TGGCTCCCAC TTTCAGCAAC CTGCTCCTGC AGCCCACCGC AGAACCTAAC 
AGTGTGGCCA GTCATAGGCC TTGTGTCAAC GGGGATATGG AGAAACCTTT AGAACCAGCC 
TCTGAGGATG GCTCTGAGTC AGAGGGGAGC GAGTCCAGCA GCCGTTCTTG TCGCAACGAG 
CGCAGCCTTC AGGAGAAGCT GCAGGCCCTG ATGGCTGAGG GCCTCCTTCC TGCTGTGAAG 
GTCTTCCTGG ACTGGCTTCG AACCAACCCT GACCTCATCA TTGTGTGTGC GCAGAGCTCT 
CAAAGTTTGT GGAACCGCCT GTCTGTGTTG CTGAATCTGT TGCCAGCATC TGGTGAGCTC 
CAGGATTCTG GCCTGGCTCT GTGTTCTGAA GTCCAAGGTC TCCTTGAAGG CTGCGAGCTG 
CCTGACCTCC CTGCTAGCCT GTTGCTCCCA GAGGACATGG CACTGCGTAA CCTACCTCCT 
CTCCGGGCTG CTCATAGACG CTTTAACTTC GATGCAGATC GGCCCCTGCT CAGTGCTTTA 
GAGGAGTCGG TGGTGCGCAT CTGCTATATC CGCAGCTTCG GGCATTTTGT TGCTCGCTTA 
CAAGGCAGCA TCCTGCAGTT CAATCCAGAG GTTGGCATCT TCGTCAGCAT TGCTCAGTCT 
GAGCAGGAGA GCCTGCTGCA GCAGGCTCAG GCACAGTTCC GTATGGCAGA GGAGGAAGCT 
CGGCGGAACA GGCTCATGAG AGACATGGCT CAGCTACGAT TACAGCTCGA GGTCTCCCAA 
CTGGAAGGGA GCCTGCAGCA GCCCAAAGCC CAGTCAGCCA TGTCTCCCTA CCTCATCCCT 
GACACCCAGG CCCTCTGCTA CCACCTCCCT GTCATCCGTC AGCTCGCCAC CAGCGGCCGC 
TTCATCATCA TCATTCCAAG GACAGTGATT GATGGTCTGG ATTTGCTGAA GAAAGAACAG 
CCAGGGGCCC GGGACGGGAT CCGGTACCTG GAGGCAGAGT TTAAAAAGGG AAACAGATAC 
ATTCGCTGCC AGAAGGAGGT GGGAAAGAGC TTTGAGCGGC ATAAGCTGAA GCGGCAGGAT 
GCAGATGCCT GGACTCTCTA TAAGATCCTG GACAGCTGCA GACAGCTAAC CCTAGCCCAG 
GGGGCAGGGG AGGAGGACCC AAGTGGCATG GTGACCATTA TCACAGGCCT TCATTTGGAC 
AACCCCAGTG CACTCTCTGG GCCCATGCAG GCTGCCCTAC AAGCTGCTGC CCATGCCAGT 
GTGGACGTCA AGAATGTTCT AGACTTCTAC AGGCAGTGGA AGGAGATTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001059906.3
CDS	141..3194
Translation	MSQGPPPVESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENVSLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYSVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKSETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPSLGLTNEDEEECESGYAFLPDLLIFQMAIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVSAFQSDGTDEPESKEALEKEEPEPEPPTVVPQADEGRKSRKHSRLSCLRRRRRHHPPKAGDDSDLSEGFESDSSHDSAQASDGSDSGSDKSLEGRGTAFDAETDSEMNSQESRSDLEDIEDEEGTRSPAQEPPQTRSEVPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTAEPNSVASHRPCVNGDMEKPLEPASEDGSESEGSESSSRSCRNERSLQEKLQALMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPASGELQDSGLALCSEVQGLLEGCELPDLPASLLLPEDMALRNLPPLRAAHRRFNFDADRPLLSALEESVVRICYIRSFGHFVARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAEEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLIPDTQALCYHLPVIRQLATSGRFIIIIPRTVIDGLDLLKKEQPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCRQLTLAQGAGEEDPSGMVTIITGLHLDNPSALSGPMQAALQAAAHASVDVKNVLDFYRQWKEIG

Target ORF information:

RefSeq Version	XM_001059906.6
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG5 nonsense mediated mRNA decay factor (Smg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001059906.6

ORF Insert Sequence:

ATGAGCCAAG GCCCTCCTCC AGTGGAGAGC AGCGAACCTG AAGCAAAGGT CCTCCACACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGGC TAGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAGGAAGT GTTTAAACCA GAGAACGTTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGTG TGAAGCTTAT GTTCTTGCAC CCAGTAGACT ATGGGAGGAA GGCTGAGGAA 
CTGCTGTGGA GAAAGGTATA CTATGAAGTC ATCCAACTCA TCAAGACTAA CAAAAAGCAC 
ATCCACAGTC GGAGCACCTT GGAATGTGCC TACAGGACTC ACCTGGTCGC CGGCATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAA TCCCACTACC AGCTGGAACT ACAGTGCTGC 
ATTGACTGGA CTCATGTCAC TGATCCCCTC ATAGGATGTA AGAAGCCAGT ATCTGCTTCA 
GGAAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
CTGTCACGCT ATCAGAATGA ACTGGCTGGT GTGGACACTG AGCTGCTAGC TGAGAGATTC 
TACTATCAAG CCTTGTCAGT CGCGCCCCAG ATTGGAATGC CCTTCAACCA GCTGGGCACT 
CTTGCAGGCA GCAAGTATTA CAGTGTGGAA GCCATGTACT GTTACTTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGA GACTCTATGA CAAGGCAGCA 
AAAATGTACC ACCAGTTGAA GAAGTCTGAG ACCAGGAAGC TCTCCCCTAG CAAAAAGCGA 
TGCAAAGACA TTAAGAGGTT GTTGGTGAAC TTCATGTACC TACAAAGCCT GTTACAGCCC 
AAAAGTAGCT CTGTGGACTC AGAACTGACC TCACTTTGCC AGTCAGTCCT AGAGGATTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAGCCTTG GTCTGACGAA CGAGGATGAG 
GAGGAGTGTG AGAGTGGATA TGCTTTCCTG CCCGACCTGC TCATCTTTCA GATGGCCATC 
ATCTGCCTTA TGGGTGTGCA CAGTTTAAAA AGAGCAGGAT CCAAGCAGTA TAGTGCAGCC 
ATTGCTTTCA CCCTGGCCCT CTTCTCCCAC CTTATTAATC ATGTCAACAT ACGACTGCAG 
GCAGAGCTGG AAGAGGGCGA GAACCCTGTC TCAGCTTTTC AGAGTGATGG CACAGATGAA 
CCAGAGTCAA AAGAAGCACT AGAAAAAGAG GAGCCAGAGC CAGAGCCTCC CACTGTGGTG 
CCCCAAGCCG ATGAGGGTAG AAAGAGTCGT AAGCACTCCC GACTCTCTTG TCTTCGTCGC 
CGCCGCCGCC ACCACCCTCC TAAGGCTGGT GATGACAGTG ACCTGAGTGA GGGTTTTGAG 
TCAGACTCTA GCCATGACTC TGCCCAGGCC AGTGATGGAT CAGACAGTGG CTCTGACAAG 
AGCCTGGAAG GCAGGGGCAC TGCTTTTGAT GCAGAAACAG ACTCAGAGAT GAACAGCCAG 
GAGTCCCGGT CAGACCTGGA AGATATAGAG GATGAAGAGG GGACACGGTC TCCAGCCCAG 
GAGCCCCCTC AGACCAGATC AGAGGTTCCA GATTCCCTCA ATGGCCCCTT GGGCCCTAGT 
GAGGCAAGCA TTGCCAGCAA TTTACAAGCC ATGTCCACCC AGATGTTCCA GACCAAGCGC 
TGCTTCCGAC TGGCTCCCAC TTTCAGCAAC CTGCTCCTGC AGCCCACCGC AGAACCTAAC 
AGTGTGGCCA GTCATAGGCC TTGTGTCAAC GGGGATATGG AGAAACCTTT AGAACCAGCC 
TCTGAGGATG GCTCTGAGTC AGAGGGGAGC GAGTCCAGCA GCCGTTCTTG TCGCAACGAG 
CGCAGCCTTC AGGAGAAGCT GCAGGCCCTG ATGGCTGAGG GCCTCCTTCC TGCTGTGAAG 
GTCTTCCTGG ACTGGCTTCG AACCAACCCT GACCTCATCA TTGTGTGTGC GCAGAGCTCT 
CAAAGTTTGT GGAACCGCCT GTCTGTGTTG CTGAATCTGT TGCCAGCATC TGGTGAGCTC 
CAGGATTCTG GCCTGGCTCT GTGTTCTGAA GTCCAAGGTC TCCTTGAAGG CTGCGAGCTG 
CCTGACCTCC CTGCTAGCCT GTTGCTCCCA GAGGACATGG CACTGCGTAA CCTACCTCCT 
CTCCGGGCTG CTCATAGACG CTTTAACTTC GATGCAGATC GGCCCCTGCT CAGTGCTTTA 
GAGGAGTCGG TGGTGCGCAT CTGCTATATC CGCAGCTTCG GGCATTTTGT TGCTCGCTTA 
CAAGGCAGCA TCCTGCAGTT CAATCCAGAG GTTGGCATCT TCGTCAGCAT TGCTCAGTCT 
GAGCAGGAGA GCCTGCTGCA GCAGGCTCAG GCACAGTTCC GTATGGCAGA GGAGGAAGCT 
CGGCGGAACA GGCTCATGAG AGACATGGCT CAGCTACGAT TACAGCTCGA GGTCTCCCAA 
CTGGAAGGGA GCCTGCAGCA GCCCAAAGCC CAGTCAGCCA TGTCTCCCTA CCTCATCCCT 
GACACCCAGG CCCTCTGCTA CCACCTCCCT GTCATCCGTC AGCTCGCCAC CAGCGGCCGC 
TTCATCATCA TCATTCCAAG GACAGTGATT GATGGTCTGG ATTTGCTGAA GAAAGAACAG 
CCAGGGGCCC GGGACGGGAT CCGGTACCTG GAGGCAGAGT TTAAAAAGGG AAACAGATAC 
ATTCGCTGCC AGAAGGAGGT GGGAAAGAGC TTTGAGCGGC ATAAGCTGAA GCGGCAGGAT 
GCAGATGCCT GGACTCTCTA TAAGATCCTG GACAGCTGCA GACAGCTAAC CCTAGCCCAG 
GGGGCAGGGG AGGAGGACCC AAGTGGCATG GTGACCATTA TCACAGGCCT TCATTTGGAC 
AACCCCAGTG CACTCTCTGG GCCCATGCAG GCTGCCCTAC AAGCTGCTGC CCATGCCAGT 
GTGGACGTCA AGAATGTTCT AGACTTCTAC AGGCAGTGGA AGGAGATTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Smg5 ( XM_002725989.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_002726035.1 Rattus norvegicus SMG5 nonsense mediated mRNA decay factor (Smg5), mRNA.

Price & Availability↑

CloneID	ORa15283
Clone ID Related Accession (Same CDS sequence)	XM_001059906.6 , XM_002725989.5
Accession Version	XM_002725989.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein SMG5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000070.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_002725989.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCAAG GCCCTCCTCC AGTGGAGAGC AGCGAACCTG AAGCAAAGGT CCTCCACACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGGC TAGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAGGAAGT GTTTAAACCA GAGAACGTTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGTG TGAAGCTTAT GTTCTTGCAC CCAGTAGACT ATGGGAGGAA GGCTGAGGAA 
CTGCTGTGGA GAAAGGTATA CTATGAAGTC ATCCAACTCA TCAAGACTAA CAAAAAGCAC 
ATCCACAGTC GGAGCACCTT GGAATGTGCC TACAGGACTC ACCTGGTCGC CGGCATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAA TCCCACTACC AGCTGGAACT ACAGTGCTGC 
ATTGACTGGA CTCATGTCAC TGATCCCCTC ATAGGATGTA AGAAGCCAGT ATCTGCTTCA 
GGAAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
CTGTCACGCT ATCAGAATGA ACTGGCTGGT GTGGACACTG AGCTGCTAGC TGAGAGATTC 
TACTATCAAG CCTTGTCAGT CGCGCCCCAG ATTGGAATGC CCTTCAACCA GCTGGGCACT 
CTTGCAGGCA GCAAGTATTA CAGTGTGGAA GCCATGTACT GTTACTTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGA GACTCTATGA CAAGGCAGCA 
AAAATGTACC ACCAGTTGAA GAAGTCTGAG ACCAGGAAGC TCTCCCCTAG CAAAAAGCGA 
TGCAAAGACA TTAAGAGGTT GTTGGTGAAC TTCATGTACC TACAAAGCCT GTTACAGCCC 
AAAAGTAGCT CTGTGGACTC AGAACTGACC TCACTTTGCC AGTCAGTCCT AGAGGATTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAGCCTTG GTCTGACGAA CGAGGATGAG 
GAGGAGTGTG AGAGTGGATA TGCTTTCCTG CCCGACCTGC TCATCTTTCA GATGGCCATC 
ATCTGCCTTA TGGGTGTGCA CAGTTTAAAA AGAGCAGGAT CCAAGCAGTA TAGTGCAGCC 
ATTGCTTTCA CCCTGGCCCT CTTCTCCCAC CTTATTAATC ATGTCAACAT ACGACTGCAG 
GCAGAGCTGG AAGAGGGCGA GAACCCTGTC TCAGCTTTTC AGAGTGATGG CACAGATGAA 
CCAGAGTCAA AAGAAGCACT AGAAAAAGAG GAGCCAGAGC CAGAGCCTCC CACTGTGGTG 
CCCCAAGCCG ATGAGGGTAG AAAGAGTCGT AAGCACTCCC GACTCTCTTG TCTTCGTCGC 
CGCCGCCGCC ACCACCCTCC TAAGGCTGGT GATGACAGTG ACCTGAGTGA GGGTTTTGAG 
TCAGACTCTA GCCATGACTC TGCCCAGGCC AGTGATGGAT CAGACAGTGG CTCTGACAAG 
AGCCTGGAAG GCAGGGGCAC TGCTTTTGAT GCAGAAACAG ACTCAGAGAT GAACAGCCAG 
GAGTCCCGGT CAGACCTGGA AGATATAGAG GATGAAGAGG GGACACGGTC TCCAGCCCAG 
GAGCCCCCTC AGACCAGATC AGAGGTTCCA GATTCCCTCA ATGGCCCCTT GGGCCCTAGT 
GAGGCAAGCA TTGCCAGCAA TTTACAAGCC ATGTCCACCC AGATGTTCCA GACCAAGCGC 
TGCTTCCGAC TGGCTCCCAC TTTCAGCAAC CTGCTCCTGC AGCCCACCGC AGAACCTAAC 
AGTGTGGCCA GTCATAGGCC TTGTGTCAAC GGGGATATGG AGAAACCTTT AGAACCAGCC 
TCTGAGGATG GCTCTGAGTC AGAGGGGAGC GAGTCCAGCA GCCGTTCTTG TCGCAACGAG 
CGCAGCCTTC AGGAGAAGCT GCAGGCCCTG ATGGCTGAGG GCCTCCTTCC TGCTGTGAAG 
GTCTTCCTGG ACTGGCTTCG AACCAACCCT GACCTCATCA TTGTGTGTGC GCAGAGCTCT 
CAAAGTTTGT GGAACCGCCT GTCTGTGTTG CTGAATCTGT TGCCAGCATC TGGTGAGCTC 
CAGGATTCTG GCCTGGCTCT GTGTTCTGAA GTCCAAGGTC TCCTTGAAGG CTGCGAGCTG 
CCTGACCTCC CTGCTAGCCT GTTGCTCCCA GAGGACATGG CACTGCGTAA CCTACCTCCT 
CTCCGGGCTG CTCATAGACG CTTTAACTTC GATGCAGATC GGCCCCTGCT CAGTGCTTTA 
GAGGAGTCGG TGGTGCGCAT CTGCTATATC CGCAGCTTCG GGCATTTTGT TGCTCGCTTA 
CAAGGCAGCA TCCTGCAGTT CAATCCAGAG GTTGGCATCT TCGTCAGCAT TGCTCAGTCT 
GAGCAGGAGA GCCTGCTGCA GCAGGCTCAG GCACAGTTCC GTATGGCAGA GGAGGAAGCT 
CGGCGGAACA GGCTCATGAG AGACATGGCT CAGCTACGAT TACAGCTCGA GGTCTCCCAA 
CTGGAAGGGA GCCTGCAGCA GCCCAAAGCC CAGTCAGCCA TGTCTCCCTA CCTCATCCCT 
GACACCCAGG CCCTCTGCTA CCACCTCCCT GTCATCCGTC AGCTCGCCAC CAGCGGCCGC 
TTCATCATCA TCATTCCAAG GACAGTGATT GATGGTCTGG ATTTGCTGAA GAAAGAACAG 
CCAGGGGCCC GGGACGGGAT CCGGTACCTG GAGGCAGAGT TTAAAAAGGG AAACAGATAC 
ATTCGCTGCC AGAAGGAGGT GGGAAAGAGC TTTGAGCGGC ATAAGCTGAA GCGGCAGGAT 
GCAGATGCCT GGACTCTCTA TAAGATCCTG GACAGCTGCA GACAGCTAAC CCTAGCCCAG 
GGGGCAGGGG AGGAGGACCC AAGTGGCATG GTGACCATTA TCACAGGCCT TCATTTGGAC 
AACCCCAGTG CACTCTCTGG GCCCATGCAG GCTGCCCTAC AAGCTGCTGC CCATGCCAGT 
GTGGACGTCA AGAATGTTCT AGACTTCTAC AGGCAGTGGA AGGAGATTGG TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002726035.1
CDS	141..3194
Translation	MSQGPPPVESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENVSLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYSVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKSETRKLSPSKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPSLGLTNEDEEECESGYAFLPDLLIFQMAIICLMGVHSLKRAGSKQYSAAIAFTLALFSHLINHVNIRLQAELEEGENPVSAFQSDGTDEPESKEALEKEEPEPEPPTVVPQADEGRKSRKHSRLSCLRRRRRHHPPKAGDDSDLSEGFESDSSHDSAQASDGSDSGSDKSLEGRGTAFDAETDSEMNSQESRSDLEDIEDEEGTRSPAQEPPQTRSEVPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTAEPNSVASHRPCVNGDMEKPLEPASEDGSESEGSESSSRSCRNERSLQEKLQALMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPASGELQDSGLALCSEVQGLLEGCELPDLPASLLLPEDMALRNLPPLRAAHRRFNFDADRPLLSALEESVVRICYIRSFGHFVARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAEEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLIPDTQALCYHLPVIRQLATSGRFIIIIPRTVIDGLDLLKKEQPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCRQLTLAQGAGEEDPSGMVTIITGLHLDNPSALSGPMQAALQAAAHASVDVKNVLDFYRQWKEIG

Target ORF information:

RefSeq Version	XM_002725989.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SMG5 nonsense mediated mRNA decay factor (Smg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002725989.5

ORF Insert Sequence:

ATGAGCCAAG GCCCTCCTCC AGTGGAGAGC AGCGAACCTG AAGCAAAGGT CCTCCACACC 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGGC TAGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAGGAAGT GTTTAAACCA GAGAACGTTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGTG TGAAGCTTAT GTTCTTGCAC CCAGTAGACT ATGGGAGGAA GGCTGAGGAA 
CTGCTGTGGA GAAAGGTATA CTATGAAGTC ATCCAACTCA TCAAGACTAA CAAAAAGCAC 
ATCCACAGTC GGAGCACCTT GGAATGTGCC TACAGGACTC ACCTGGTCGC CGGCATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAA TCCCACTACC AGCTGGAACT ACAGTGCTGC 
ATTGACTGGA CTCATGTCAC TGATCCCCTC ATAGGATGTA AGAAGCCAGT ATCTGCTTCA 
GGAAAGGAGA TGGATTGGGC ACAGATGGCA TGCCACCGAT GTCTGGTGTA CCTGGGGGAT 
CTGTCACGCT ATCAGAATGA ACTGGCTGGT GTGGACACTG AGCTGCTAGC TGAGAGATTC 
TACTATCAAG CCTTGTCAGT CGCGCCCCAG ATTGGAATGC CCTTCAACCA GCTGGGCACT 
CTTGCAGGCA GCAAGTATTA CAGTGTGGAA GCCATGTACT GTTACTTGCG CTGCATCCAG 
TCGGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGA GACTCTATGA CAAGGCAGCA 
AAAATGTACC ACCAGTTGAA GAAGTCTGAG ACCAGGAAGC TCTCCCCTAG CAAAAAGCGA 
TGCAAAGACA TTAAGAGGTT GTTGGTGAAC TTCATGTACC TACAAAGCCT GTTACAGCCC 
AAAAGTAGCT CTGTGGACTC AGAACTGACC TCACTTTGCC AGTCAGTCCT AGAGGATTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAGCCTTG GTCTGACGAA CGAGGATGAG 
GAGGAGTGTG AGAGTGGATA TGCTTTCCTG CCCGACCTGC TCATCTTTCA GATGGCCATC 
ATCTGCCTTA TGGGTGTGCA CAGTTTAAAA AGAGCAGGAT CCAAGCAGTA TAGTGCAGCC 
ATTGCTTTCA CCCTGGCCCT CTTCTCCCAC CTTATTAATC ATGTCAACAT ACGACTGCAG 
GCAGAGCTGG AAGAGGGCGA GAACCCTGTC TCAGCTTTTC AGAGTGATGG CACAGATGAA 
CCAGAGTCAA AAGAAGCACT AGAAAAAGAG GAGCCAGAGC CAGAGCCTCC CACTGTGGTG 
CCCCAAGCCG ATGAGGGTAG AAAGAGTCGT AAGCACTCCC GACTCTCTTG TCTTCGTCGC 
CGCCGCCGCC ACCACCCTCC TAAGGCTGGT GATGACAGTG ACCTGAGTGA GGGTTTTGAG 
TCAGACTCTA GCCATGACTC TGCCCAGGCC AGTGATGGAT CAGACAGTGG CTCTGACAAG 
AGCCTGGAAG GCAGGGGCAC TGCTTTTGAT GCAGAAACAG ACTCAGAGAT GAACAGCCAG 
GAGTCCCGGT CAGACCTGGA AGATATAGAG GATGAAGAGG GGACACGGTC TCCAGCCCAG 
GAGCCCCCTC AGACCAGATC AGAGGTTCCA GATTCCCTCA ATGGCCCCTT GGGCCCTAGT 
GAGGCAAGCA TTGCCAGCAA TTTACAAGCC ATGTCCACCC AGATGTTCCA GACCAAGCGC 
TGCTTCCGAC TGGCTCCCAC TTTCAGCAAC CTGCTCCTGC AGCCCACCGC AGAACCTAAC 
AGTGTGGCCA GTCATAGGCC TTGTGTCAAC GGGGATATGG AGAAACCTTT AGAACCAGCC 
TCTGAGGATG GCTCTGAGTC AGAGGGGAGC GAGTCCAGCA GCCGTTCTTG TCGCAACGAG 
CGCAGCCTTC AGGAGAAGCT GCAGGCCCTG ATGGCTGAGG GCCTCCTTCC TGCTGTGAAG 
GTCTTCCTGG ACTGGCTTCG AACCAACCCT GACCTCATCA TTGTGTGTGC GCAGAGCTCT 
CAAAGTTTGT GGAACCGCCT GTCTGTGTTG CTGAATCTGT TGCCAGCATC TGGTGAGCTC 
CAGGATTCTG GCCTGGCTCT GTGTTCTGAA GTCCAAGGTC TCCTTGAAGG CTGCGAGCTG 
CCTGACCTCC CTGCTAGCCT GTTGCTCCCA GAGGACATGG CACTGCGTAA CCTACCTCCT 
CTCCGGGCTG CTCATAGACG CTTTAACTTC GATGCAGATC GGCCCCTGCT CAGTGCTTTA 
GAGGAGTCGG TGGTGCGCAT CTGCTATATC CGCAGCTTCG GGCATTTTGT TGCTCGCTTA 
CAAGGCAGCA TCCTGCAGTT CAATCCAGAG GTTGGCATCT TCGTCAGCAT TGCTCAGTCT 
GAGCAGGAGA GCCTGCTGCA GCAGGCTCAG GCACAGTTCC GTATGGCAGA GGAGGAAGCT 
CGGCGGAACA GGCTCATGAG AGACATGGCT CAGCTACGAT TACAGCTCGA GGTCTCCCAA 
CTGGAAGGGA GCCTGCAGCA GCCCAAAGCC CAGTCAGCCA TGTCTCCCTA CCTCATCCCT 
GACACCCAGG CCCTCTGCTA CCACCTCCCT GTCATCCGTC AGCTCGCCAC CAGCGGCCGC 
TTCATCATCA TCATTCCAAG GACAGTGATT GATGGTCTGG ATTTGCTGAA GAAAGAACAG 
CCAGGGGCCC GGGACGGGAT CCGGTACCTG GAGGCAGAGT TTAAAAAGGG AAACAGATAC 
ATTCGCTGCC AGAAGGAGGT GGGAAAGAGC TTTGAGCGGC ATAAGCTGAA GCGGCAGGAT 
GCAGATGCCT GGACTCTCTA TAAGATCCTG GACAGCTGCA GACAGCTAAC CCTAGCCCAG 
GGGGCAGGGG AGGAGGACCC AAGTGGCATG GTGACCATTA TCACAGGCCT TCATTTGGAC 
AACCCCAGTG CACTCTCTGG GCCCATGCAG GCTGCCCTAC AAGCTGCTGC CCATGCCAGT 
GTGGACGTCA AGAATGTTCT AGACTTCTAC AGGCAGTGGA AGGAGATTGG TTGA