SMG5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMG5
Entrez Gene ID	23381
Full Name	SMG5, nonsense mediated mRNA decay factor
Synonyms	EST1B,LPTS-RP1,LPTSRP1,SMG-5
General protein information	Preferred Names SMG5, nonsense mediated mRNA decay factor Names protein SMG5 EST1 telomerase component homolog B EST1-like protein B Est1p-like protein B LPTS interacting protein ever shorter telomeres 1B smg-5 homolog, nonsense mediated mRNA decay factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q22
Summary	SMG5 is involved in nonsense-mediated mRNA decay (Ohnishi et al., 2003 [PubMed 14636577]).[supplied by OMIM, Mar 2008].
Disorder MIM:	610962

mRNA and Protein(s)

mRNA	Protein	Name
XM_017000842.1	XP_016856331.1	protein SMG5 isoform X1
NM_001323614.1	NP_001310543.1	protein SMG5 isoform 2
XM_017000843.1	XP_016856332.1	protein SMG5 isoform X2
NM_015327.2	NP_056142.2	protein SMG5 isoform 1
NM_001323617.1	NP_001310546.1	protein SMG5 isoform 4
NM_015327.3	NP_056142.2	protein SMG5 isoform 1
NM_001323615.1	NP_001310544.1	protein SMG5 isoform 3
NM_001323616.1	NP_001310545.1	protein SMG5 isoform 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMG5	XP_513880.3
	SMG5	XP_002801899.1
Canis lupus familiaris (dog)	SMG5	XP_547535.3
Bos taurus (cattle)	SMG5	NP_001077148.1
Rattus norvegicus (Norway rat)	Smg5	XP_001059906.3
Gallus gallus (chicken)	SMG5	XP_423228.4
Xenopus tropicalis (tropical clawed frog)	smg5	NP_001072622.1
Homo sapiens (human)	SMG5	NP_056142.2
Mus musculus (house mouse)	Smg5	NP_839977.2

Gene Ontology
Bibliography

Related articles in PubMed

Dissecting the functions of SMG5, SMG7 and PNRC2 in nonsense-mediated mRNA decay of human cells.
Nicholson P, Gkratsou A, Josi C, Colombo M, M?hlemann O
RNA (New York, N.Y.)(2018 Jan)

MicroRNA 433 regulates nonsense-mediated mRNA decay by targeting SMG5 mRNA.
Jin Y, Zhang F, Ma Z, Ren Z
BMC molecular biology17(1)17(2016 07)

The host nonsense-mediated mRNA decay pathway restricts Mammalian RNA virus replication.
Balistreri G, Horvath P, Schweingruber C, Z?nd D, McInerney G, Merits A, M?hlemann O, Azzalin C, Helenius A
Cell host & microbe16(3)403-11(2014 Sep)

Phospho-dependent and phospho-independent interactions of the helicase UPF1 with the NMD factors SMG5-SMG7 and SMG6.
Chakrabarti S, Bonneau F, Sch?ssler S, Eppinger E, Conti E
Nucleic acids research42(14)9447-60(2014 Aug)

Multiple nonglycemic genomic loci are newly associated with blood level of glycated hemoglobin in East Asians.
Chen P, Takeuchi F, Lee JY, Li H, Wu JY, Liang J, Long J, Tabara Y, Goodarzi MO, Pereira MA, Kim YJ, Go MJ, Stram DO, Vithana E, Khor CC, Liu J, Liao J, Ye X, Wang Y, Lu L, Young TL, Lee J, Thai AC, Cheng CY, van Dam RM, Friedlander Y, Heng CK, Koh WP, Chen CH, Chang LC, Pan WH, Qi Q, Isono M, Zheng W, Cai Q, Gao Y, Yamamoto K, Ohnaka K, Takayanagi R, Kita Y, Ueshima H, Hsiung CA, Cui J, Sheu WH, Rotter JI, Chen YD, Hsu C, Okada Y, Kubo M, Takahashi A, Tanaka T, van Rooij FJ, Ganesh SK, Huang J, Huang T, Yuan J, Hwang JY, , Gross MD, Assimes TL, Miki T, Shu XO, Qi L, Chen YT, Lin X, Aung T, Wong TY, Teo YY, Kim BJ, Kato N, Tai ES
Diabetes63(7)2551-62(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study highlights that miR-433 represses nonsense mediated mRNA decay. The miR-433 targets 3'-UTR of SMG5 and represses the expression of SMG5, whereas nonsense-mediated mRNA decay activity is decreased when SMG5 is decreased.
Title: MicroRNA 433 regulates nonsense-mediated mRNA decay by targeting SMG5 mRNA.

Depletion of nonsense-mediated mRNA decay pathway components Upf1, Smg5, and Smg7 led to increased levels of viral proteins and and virus release.
Title: The host nonsense-mediated mRNA decay pathway restricts Mammalian RNA virus replication.

The study demonstrates that SMG5-SMG7 and SMG6 exhibit different and non-overlapping modes of UPF1 recognition, thus pointing at distinguished roles in integrating the complex nonsense-mediated mRNA decay interaction network.
Title: Phospho-dependent and phospho-independent interactions of the helicase UPF1 with the NMD factors SMG5-SMG7 and SMG6.

HDAC8 regulation of hEST1B protein stability modulates total telomerase enzymatic activity
Title: Histone deacetylase 8 safeguards the human ever-shorter telomeres 1B (hEST1B) protein from ubiquitin-mediated degradation.

Data show that phosphorylated hUPF1, the human ortholog of UPF1/SMG-2, forms a complex with human orthologs of the Caenorhabditis elegans proteins SMG-5 and SMG-7.
Title: Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7.

The following SMG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu34276	NM_001323615.1 Latest version!	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu34277	NM_001323617.1 Latest version!	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu34277	NM_001323616.1 Latest version!	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu66116	NM_001323614.1 Latest version!	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu87131	XM_017000842.1 Latest version!	Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87132	XM_017000843.1 Latest version!	Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu15477	NM_015327.3 Latest version!	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
View Old Accession Versions >>
OHu15477	NM_015327.2	Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMG5 ( NM_001323615.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310544.1 Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu34276
Clone ID Related Accession (Same CDS sequence)	NM_001323615.1
Accession Version	NM_001323615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	protein SMG5 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL589685.17 and AL135927.14. On Apr 22, 2016 this sequence version replaced XM_005245049.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.215702.1, SRR1803617.9959.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCCG AAGCAAAAGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT ATTCAAACCA GAAAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTGGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTATA CTATGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAC 
ATCCACAGCC GGAGCACTTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCCCACTACC AGCTGGAACT GCAGTGCTGC 
ATCGACTGGA CCCATGTCAC TGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGTCACCGAT GTCTGGTGTA TCTGGGGGAT 
TTGTCCCGAT ATCAGAATGA ATTAGCTGGC GTAGATACCG AGCTGCTAGC CGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAATCA GCTGGGCACC 
CTGGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATCCAG 
TCAGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAAATGTACC ACCAACTGAA GAAGTGTGAG ACTCGGAAAC TGTCTCCTGG CAAAAAGCGA 
TGTAAAGACA TTAAAAGGTT GCTAGTGAAC TTTATGTATC TGCAAAGCCT CCTACAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACC TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGTGTGTGCA CAGCTTGGAG AGAGCAGATG AACCAGAGTC CAAGGAACCT 
GTGGAGAAAG AGGAGGAGCC AGATCCTGAG CCTCCTCCTG TAACACCCCA AGTGGGTGAG 
GGCAGAAAGA GCCGTAAGTT CTCTCGCCTC TCCTGTCTCC GCCGTCGCCG CCACCCACCC 
AAAGTTGGTG ATGACAGTGA CCTGAGTGAA GGCTTTGAAT CGGACTCAAG CCATGACTCA 
GCCCGGGCCA GTGAGGGCTC AGACAGTGGC TCTGACAAGA GTCTTGAAGG TGGGGGAACG 
GCCTTTGATG CTGAAACAGA CTCGGAAATG AATAGCCAGG AGTCCCGATC AGACTTGGAA 
GATATGGAGG AAGAGGAGGG GACACGGTCA CCAACCCTGG AGCCCCCTCG GGGCAGATCA 
GAGGCTCCCG ATTCCCTCAA TGGCCCACTG GGCCCCAGTG AGGCTAGCAT TGCCAGCAAT 
CTACAAGCCA TGTCCACCCA GATGTTCCAG ACTAAGCGCT GCTTCCGACT GGCCCCCACC 
TTTAGCAACC TGCTCCTCCA GCCCACCACC AACCCTCATA CCTCGGCCAG CCACAGGCCT 
TGCGTCAATG GGGATGTAGA CAAGCCTTCA GAGCCAGCCT CTGAGGAGGG CTCTGAGTCG 
GAGGGGAGTG AGTCCAGTGG ACGCTCCTGT CGGAATGAGC GCAGCATCCA GGAGAAGCTT 
CAGGTCCTGA TGGCCGAAGG TCTGCTTCCT GCTGTGAAAG TCTTCCTGGA CTGGCTTCGG 
ACCAACCCCG ACCTCATCAT CGTGTGTGCG CAGAGCTCTC AAAGTCTGTG GAACCGCCTG 
TCTGTGTTGC TGAATCTGTT GCCTGCTGCT GGTGAACTCC AGGAGTCTGG CCTGGCCTTG 
TGTCCTGAGG TCCAAGATCT TCTTGAAGGT TGTGAACTGC CTGACCTCCC CTCTAGCCTT 
CTGCTCCCAG AGGACATGGC TCTTCGTAAC CTGCCCCCGC TCCGAGCTGC CCACAGACGC 
TTTAACTTTG ACACGGATCG GCCCCTGCTC AGCACCTTAG AGGAGTCAGT GGTGCGCATC 
TGCTGCATCC GCAGCTTTGG TCATTTCATC GCCCGCCTGC AAGGCAGCAT CCTGCAGTTC 
AACCCAGAGG TTGGCATCTT CGTCAGCATT GCCCAGTCTG AGCAGGAGAG CCTGCTGCAG 
CAGGCCCAGG CACAGTTCCG AATGGCACAG GAGGAAGCTC GTCGGAACAG GCTCATGAGA 
GACATGGCTC AGCTACGACT TCAGCTCGAA GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG 
CCCAAGGCCC AGTCAGCCAT GTCTCCCTAC CTCGTCCCTG ACACCCAGGC CCTCTGCCAC 
CATCTCCCTG TCATCCGCCA ACTGGCCACC AGTGGCCGCT TCATTGTCAT CATCCCAAGG 
ACAGTGATCG ATGGCCTGGA TTTGCTGAAG AAGGAACACC CAGGGGCCCG GGATGGGATT 
CGGTACCTGG AGGCAGAGTT TAAAAAAGGA AACAGGTACA TTCGCTGCCA GAAAGAGGTG 
GGAAAGAGCT TTGAGCGGCA TAAGCTGAAG AGGCAGGATG CAGATGCCTG GACTCTCTAT 
AAGATCCTAG ACAGCTGCAA ACAGCTGACT CTGGCCCAGG GGGCAGGTGA GGAGGATCCG 
AGTGGCATGG TGACCATCAT CACAGGCCTT CCACTGGACA ACCCCAGCGT GCTTTCAGGC 
CCCATGCAGG CAGCCCTGCA GGCCGCTGCC CACGCCAGTG TGGACATCAA GAATGTTCTG 
GACTTCTACA AGCAGTGGAA GGAAATTGGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310544.1
CDS	211..3123
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERADEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	NM_001323615.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323615.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCCG AAGCAAAAGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT ATTCAAACCA GAAAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTGGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTATA CTATGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAC 
ATCCACAGCC GGAGCACTTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCCCACTACC AGCTGGAACT GCAGTGCTGC 
ATCGACTGGA CCCATGTCAC TGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGTCACCGAT GTCTGGTGTA TCTGGGGGAT 
TTGTCCCGAT ATCAGAATGA ATTAGCTGGC GTAGATACCG AGCTGCTAGC CGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAATCA GCTGGGCACC 
CTGGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATCCAG 
TCAGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAAATGTACC ACCAACTGAA GAAGTGTGAG ACTCGGAAAC TGTCTCCTGG CAAAAAGCGA 
TGTAAAGACA TTAAAAGGTT GCTAGTGAAC TTTATGTATC TGCAAAGCCT CCTACAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACC TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGTGTGTGCA CAGCTTGGAG AGAGCAGATG AACCAGAGTC CAAGGAACCT 
GTGGAGAAAG AGGAGGAGCC AGATCCTGAG CCTCCTCCTG TAACACCCCA AGTGGGTGAG 
GGCAGAAAGA GCCGTAAGTT CTCTCGCCTC TCCTGTCTCC GCCGTCGCCG CCACCCACCC 
AAAGTTGGTG ATGACAGTGA CCTGAGTGAA GGCTTTGAAT CGGACTCAAG CCATGACTCA 
GCCCGGGCCA GTGAGGGCTC AGACAGTGGC TCTGACAAGA GTCTTGAAGG TGGGGGAACG 
GCCTTTGATG CTGAAACAGA CTCGGAAATG AATAGCCAGG AGTCCCGATC AGACTTGGAA 
GATATGGAGG AAGAGGAGGG GACACGGTCA CCAACCCTGG AGCCCCCTCG GGGCAGATCA 
GAGGCTCCCG ATTCCCTCAA TGGCCCACTG GGCCCCAGTG AGGCTAGCAT TGCCAGCAAT 
CTACAAGCCA TGTCCACCCA GATGTTCCAG ACTAAGCGCT GCTTCCGACT GGCCCCCACC 
TTTAGCAACC TGCTCCTCCA GCCCACCACC AACCCTCATA CCTCGGCCAG CCACAGGCCT 
TGCGTCAATG GGGATGTAGA CAAGCCTTCA GAGCCAGCCT CTGAGGAGGG CTCTGAGTCG 
GAGGGGAGTG AGTCCAGTGG ACGCTCCTGT CGGAATGAGC GCAGCATCCA GGAGAAGCTT 
CAGGTCCTGA TGGCCGAAGG TCTGCTTCCT GCTGTGAAAG TCTTCCTGGA CTGGCTTCGG 
ACCAACCCCG ACCTCATCAT CGTGTGTGCG CAGAGCTCTC AAAGTCTGTG GAACCGCCTG 
TCTGTGTTGC TGAATCTGTT GCCTGCTGCT GGTGAACTCC AGGAGTCTGG CCTGGCCTTG 
TGTCCTGAGG TCCAAGATCT TCTTGAAGGT TGTGAACTGC CTGACCTCCC CTCTAGCCTT 
CTGCTCCCAG AGGACATGGC TCTTCGTAAC CTGCCCCCGC TCCGAGCTGC CCACAGACGC 
TTTAACTTTG ACACGGATCG GCCCCTGCTC AGCACCTTAG AGGAGTCAGT GGTGCGCATC 
TGCTGCATCC GCAGCTTTGG TCATTTCATC GCCCGCCTGC AAGGCAGCAT CCTGCAGTTC 
AACCCAGAGG TTGGCATCTT CGTCAGCATT GCCCAGTCTG AGCAGGAGAG CCTGCTGCAG 
CAGGCCCAGG CACAGTTCCG AATGGCACAG GAGGAAGCTC GTCGGAACAG GCTCATGAGA 
GACATGGCTC AGCTACGACT TCAGCTCGAA GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG 
CCCAAGGCCC AGTCAGCCAT GTCTCCCTAC CTCGTCCCTG ACACCCAGGC CCTCTGCCAC 
CATCTCCCTG TCATCCGCCA ACTGGCCACC AGTGGCCGCT TCATTGTCAT CATCCCAAGG 
ACAGTGATCG ATGGCCTGGA TTTGCTGAAG AAGGAACACC CAGGGGCCCG GGATGGGATT 
CGGTACCTGG AGGCAGAGTT TAAAAAAGGA AACAGGTACA TTCGCTGCCA GAAAGAGGTG 
GGAAAGAGCT TTGAGCGGCA TAAGCTGAAG AGGCAGGATG CAGATGCCTG GACTCTCTAT 
AAGATCCTAG ACAGCTGCAA ACAGCTGACT CTGGCCCAGG GGGCAGGTGA GGAGGATCCG 
AGTGGCATGG TGACCATCAT CACAGGCCTT CCACTGGACA ACCCCAGCGT GCTTTCAGGC 
CCCATGCAGG CAGCCCTGCA GGCCGCTGCC CACGCCAGTG TGGACATCAA GAATGTTCTG 
GACTTCTACA AGCAGTGGAA GGAAATTGGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( NM_001323617.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310546.1 Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu34277
Clone ID Related Accession (Same CDS sequence)	NM_001323617.1 , NM_001323616.1
Accession Version	NM_001323617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	protein SMG5 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL589685.17 and AL135927.14. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.154274.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTGC ACCCAGTGGA CTATGGGAGA AAGGCTGAGG AGCTGCTGTG GAGAAAGGTA 
TACTATGAAG TTATCCAGCT TATCAAGACT AACAAAAAGC ACATCCACAG CCGGAGCACT 
TTGGAATGTG CCTACAGGAC GCACCTGGTT GCTGGTATTG GCTTCTACCA GCATCTCCTT 
CTCTATATCC AGTCCCACTA CCAGCTGGAA CTGCAGTGCT GCATCGACTG GACCCATGTC 
ACTGACCCCC TCATAGGATG CAAGAAGCCA GTGTCTGCCT CAGGGAAGGA GATGGATTGG 
GCACAGATGG CATGTCACCG ATGTCTGGTG TATCTGGGGG ATTTGTCCCG ATATCAGAAT 
GAATTAGCTG GCGTAGATAC CGAGCTGCTA GCCGAGAGAT TTTACTACCA AGCCCTGTCA 
GTAGCTCCTC AGATTGGAAT GCCCTTCAAT CAGCTGGGCA CCCTGGCAGG CAGCAAGTAC 
TATAATGTGG AAGCCATGTA TTGCTACCTG CGCTGCATCC AGTCAGAAGT GTCCTTTGAG 
GGAGCCTATG GGAACCTCAA GCGGCTGTAT GACAAGGCAG CCAAAATGTA CCACCAACTG 
AAGAAGTGTG AGACTCGGAA ACTGTCTCCT GGCAAAAAGC GATGTAAAGA CATTAAAAGG 
TTGCTAGTGA ACTTTATGTA TCTGCAAAGC CTCCTACAGC CCAAAAGCAG CTCCGTGGAC 
TCAGAGCTGA CCTCACTTTG CCAGTCAGTC CTGGAGGACT TCAACCTCTG CCTCTTCTAC 
CTGCCCTCCT CACCCAACCT CAGCCTGGCC AGTGAGGATG AGGAGGAGTA TGAGAGTGGA 
TATGCTTTCC TCCCGGACCT TCTCATCTTT CAAATGGTCA TCATCTGCCT TATGTGTGTG 
CACAGCTTGG AGAGAGCAGG ATCCAAGCAG TACAGTGCAG CCATTGCCTT CACCCTGGCC 
CTCTTTTCCC ACCTCGTCAA TCATGTCAAC ATACGGCTGC AGGCTGAGCT GGAAGAGGGC 
GAGAATCCCG TCCCGGCATT CCAGAGTGAT GGCACAGATG AACCAGAGTC CAAGGAACCT 
GTGGAGAAAG AGGAGGAGCC AGATCCTGAG CCTCCTCCTG TAACACCCCA AGTGGGTGAG 
GGCAGAAAGA GCCGTAAGTT CTCTCGCCTC TCCTGTCTCC GCCGTCGCCG CCACCCACCC 
AAAGTTGGTG ATGACAGTGA CCTGAGTGAA GGCTTTGAAT CGGACTCAAG CCATGACTCA 
GCCCGGGCCA GTGAGGGCTC AGACAGTGGC TCTGACAAGA GTCTTGAAGG TGGGGGAACG 
GCCTTTGATG CTGAAACAGA CTCGGAAATG AATAGCCAGG AGTCCCGATC AGACTTGGAA 
GATATGGAGG AAGAGGAGGG GACACGGTCA CCAACCCTGG AGCCCCCTCG GGGCAGATCA 
GAGGCTCCCG ATTCCCTCAA TGGCCCACTG GGCCCCAGTG AGGCTAGCAT TGCCAGCAAT 
CTACAAGCCA TGTCCACCCA GATGTTCCAG ACTAAGCGCT GCTTCCGACT GGCCCCCACC 
TTTAGCAACC TGCTCCTCCA GCCCACCACC AACCCTCATA CCTCGGCCAG CCACAGGCCT 
TGCGTCAATG GGGATGTAGA CAAGCCTTCA GAGCCAGCCT CTGAGGAGGG CTCTGAGTCG 
GAGGGGAGTG AGTCCAGTGG ACGCTCCTGT CGGAATGAGC GCAGCATCCA GGAGAAGCTT 
CAGGTCCTGA TGGCCGAAGG TCTGCTTCCT GCTGTGAAAG TCTTCCTGGA CTGGCTTCGG 
ACCAACCCCG ACCTCATCAT CGTGTGTGCG CAGAGCTCTC AAAGTCTGTG GAACCGCCTG 
TCTGTGTTGC TGAATCTGTT GCCTGCTGCT GGTGAACTCC AGGAGTCTGG CCTGGCCTTG 
TGTCCTGAGG TCCAAGATCT TCTTGAAGGT TGTGAACTGC CTGACCTCCC CTCTAGCCTT 
CTGCTCCCAG AGGACATGGC TCTTCGTAAC CTGCCCCCGC TCCGAGCTGC CCACAGACGC 
TTTAACTTTG ACACGGATCG GCCCCTGCTC AGCACCTTAG AGGAGTCAGT GGTGCGCATC 
TGCTGCATCC GCAGCTTTGG TCATTTCATC GCCCGCCTGC AAGGCAGCAT CCTGCAGTTC 
AACCCAGAGG TTGGCATCTT CGTCAGCATT GCCCAGTCTG AGCAGGAGAG CCTGCTGCAG 
CAGGCCCAGG CACAGTTCCG AATGGCACAG GAGGAAGCTC GTCGGAACAG GCTCATGAGA 
GACATGGCTC AGCTACGACT TCAGCTCGAA GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG 
CCCAAGGCCC AGTCAGCCAT GTCTCCCTAC CTCGTCCCTG ACACCCAGGC CCTCTGCCAC 
CATCTCCCTG TCATCCGCCA ACTGGCCACC AGTGGCCGCT TCATTGTCAT CATCCCAAGG 
ACAGTGATCG ATGGCCTGGA TTTGCTGAAG AAGGAACACC CAGGGGCCCG GGATGGGATT 
CGGTACCTGG AGGCAGAGTT TAAAAAAGGA AACAGGTACA TTCGCTGCCA GAAAGAGGTG 
GGAAAGAGCT TTGAGCGGCA TAAGCTGAAG AGGCAGGATG CAGATGCCTG GACTCTCTAT 
AAGATCCTAG ACAGCTGCAA ACAGCTGACT CTGGCCCAGG GGGCAGGTGA GGAGGATCCG 
AGTGGCATGG TGACCATCAT CACAGGCCTT CCACTGGACA ACCCCAGCGT GCTTTCAGGC 
CCCATGCAGG CAGCCCTGCA GGCCGCTGCC CACGCCAGTG TGGACATCAA GAATGTTCTG 
GACTTCTACA AGCAGTGGAA GGAAATTGGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310546.1
CDS	376..3228
Translation	MFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	NM_001323617.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323617.1

ORF Insert Sequence:

ATGTTCCTGC ACCCAGTGGA CTATGGGAGA AAGGCTGAGG AGCTGCTGTG GAGAAAGGTA 
TACTATGAAG TTATCCAGCT TATCAAGACT AACAAAAAGC ACATCCACAG CCGGAGCACT 
TTGGAATGTG CCTACAGGAC GCACCTGGTT GCTGGTATTG GCTTCTACCA GCATCTCCTT 
CTCTATATCC AGTCCCACTA CCAGCTGGAA CTGCAGTGCT GCATCGACTG GACCCATGTC 
ACTGACCCCC TCATAGGATG CAAGAAGCCA GTGTCTGCCT CAGGGAAGGA GATGGATTGG 
GCACAGATGG CATGTCACCG ATGTCTGGTG TATCTGGGGG ATTTGTCCCG ATATCAGAAT 
GAATTAGCTG GCGTAGATAC CGAGCTGCTA GCCGAGAGAT TTTACTACCA AGCCCTGTCA 
GTAGCTCCTC AGATTGGAAT GCCCTTCAAT CAGCTGGGCA CCCTGGCAGG CAGCAAGTAC 
TATAATGTGG AAGCCATGTA TTGCTACCTG CGCTGCATCC AGTCAGAAGT GTCCTTTGAG 
GGAGCCTATG GGAACCTCAA GCGGCTGTAT GACAAGGCAG CCAAAATGTA CCACCAACTG 
AAGAAGTGTG AGACTCGGAA ACTGTCTCCT GGCAAAAAGC GATGTAAAGA CATTAAAAGG 
TTGCTAGTGA ACTTTATGTA TCTGCAAAGC CTCCTACAGC CCAAAAGCAG CTCCGTGGAC 
TCAGAGCTGA CCTCACTTTG CCAGTCAGTC CTGGAGGACT TCAACCTCTG CCTCTTCTAC 
CTGCCCTCCT CACCCAACCT CAGCCTGGCC AGTGAGGATG AGGAGGAGTA TGAGAGTGGA 
TATGCTTTCC TCCCGGACCT TCTCATCTTT CAAATGGTCA TCATCTGCCT TATGTGTGTG 
CACAGCTTGG AGAGAGCAGG ATCCAAGCAG TACAGTGCAG CCATTGCCTT CACCCTGGCC 
CTCTTTTCCC ACCTCGTCAA TCATGTCAAC ATACGGCTGC AGGCTGAGCT GGAAGAGGGC 
GAGAATCCCG TCCCGGCATT CCAGAGTGAT GGCACAGATG AACCAGAGTC CAAGGAACCT 
GTGGAGAAAG AGGAGGAGCC AGATCCTGAG CCTCCTCCTG TAACACCCCA AGTGGGTGAG 
GGCAGAAAGA GCCGTAAGTT CTCTCGCCTC TCCTGTCTCC GCCGTCGCCG CCACCCACCC 
AAAGTTGGTG ATGACAGTGA CCTGAGTGAA GGCTTTGAAT CGGACTCAAG CCATGACTCA 
GCCCGGGCCA GTGAGGGCTC AGACAGTGGC TCTGACAAGA GTCTTGAAGG TGGGGGAACG 
GCCTTTGATG CTGAAACAGA CTCGGAAATG AATAGCCAGG AGTCCCGATC AGACTTGGAA 
GATATGGAGG AAGAGGAGGG GACACGGTCA CCAACCCTGG AGCCCCCTCG GGGCAGATCA 
GAGGCTCCCG ATTCCCTCAA TGGCCCACTG GGCCCCAGTG AGGCTAGCAT TGCCAGCAAT 
CTACAAGCCA TGTCCACCCA GATGTTCCAG ACTAAGCGCT GCTTCCGACT GGCCCCCACC 
TTTAGCAACC TGCTCCTCCA GCCCACCACC AACCCTCATA CCTCGGCCAG CCACAGGCCT 
TGCGTCAATG GGGATGTAGA CAAGCCTTCA GAGCCAGCCT CTGAGGAGGG CTCTGAGTCG 
GAGGGGAGTG AGTCCAGTGG ACGCTCCTGT CGGAATGAGC GCAGCATCCA GGAGAAGCTT 
CAGGTCCTGA TGGCCGAAGG TCTGCTTCCT GCTGTGAAAG TCTTCCTGGA CTGGCTTCGG 
ACCAACCCCG ACCTCATCAT CGTGTGTGCG CAGAGCTCTC AAAGTCTGTG GAACCGCCTG 
TCTGTGTTGC TGAATCTGTT GCCTGCTGCT GGTGAACTCC AGGAGTCTGG CCTGGCCTTG 
TGTCCTGAGG TCCAAGATCT TCTTGAAGGT TGTGAACTGC CTGACCTCCC CTCTAGCCTT 
CTGCTCCCAG AGGACATGGC TCTTCGTAAC CTGCCCCCGC TCCGAGCTGC CCACAGACGC 
TTTAACTTTG ACACGGATCG GCCCCTGCTC AGCACCTTAG AGGAGTCAGT GGTGCGCATC 
TGCTGCATCC GCAGCTTTGG TCATTTCATC GCCCGCCTGC AAGGCAGCAT CCTGCAGTTC 
AACCCAGAGG TTGGCATCTT CGTCAGCATT GCCCAGTCTG AGCAGGAGAG CCTGCTGCAG 
CAGGCCCAGG CACAGTTCCG AATGGCACAG GAGGAAGCTC GTCGGAACAG GCTCATGAGA 
GACATGGCTC AGCTACGACT TCAGCTCGAA GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG 
CCCAAGGCCC AGTCAGCCAT GTCTCCCTAC CTCGTCCCTG ACACCCAGGC CCTCTGCCAC 
CATCTCCCTG TCATCCGCCA ACTGGCCACC AGTGGCCGCT TCATTGTCAT CATCCCAAGG 
ACAGTGATCG ATGGCCTGGA TTTGCTGAAG AAGGAACACC CAGGGGCCCG GGATGGGATT 
CGGTACCTGG AGGCAGAGTT TAAAAAAGGA AACAGGTACA TTCGCTGCCA GAAAGAGGTG 
GGAAAGAGCT TTGAGCGGCA TAAGCTGAAG AGGCAGGATG CAGATGCCTG GACTCTCTAT 
AAGATCCTAG ACAGCTGCAA ACAGCTGACT CTGGCCCAGG GGGCAGGTGA GGAGGATCCG 
AGTGGCATGG TGACCATCAT CACAGGCCTT CCACTGGACA ACCCCAGCGT GCTTTCAGGC 
CCCATGCAGG CAGCCCTGCA GGCCGCTGCC CACGCCAGTG TGGACATCAA GAATGTTCTG 
GACTTCTACA AGCAGTGGAA GGAAATTGGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( NM_001323616.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310545.1 Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu34277
Clone ID Related Accession (Same CDS sequence)	NM_001323617.1 , NM_001323616.1
Accession Version	NM_001323616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	protein SMG5 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL589685.17 and AL135927.14. On Apr 22, 2016 this sequence version replaced XM_011509362.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.108478.1, SRR1803612.67373.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCCTGC ACCCAGTGGA CTATGGGAGA AAGGCTGAGG AGCTGCTGTG GAGAAAGGTA 
TACTATGAAG TTATCCAGCT TATCAAGACT AACAAAAAGC ACATCCACAG CCGGAGCACT 
TTGGAATGTG CCTACAGGAC GCACCTGGTT GCTGGTATTG GCTTCTACCA GCATCTCCTT 
CTCTATATCC AGTCCCACTA CCAGCTGGAA CTGCAGTGCT GCATCGACTG GACCCATGTC 
ACTGACCCCC TCATAGGATG CAAGAAGCCA GTGTCTGCCT CAGGGAAGGA GATGGATTGG 
GCACAGATGG CATGTCACCG ATGTCTGGTG TATCTGGGGG ATTTGTCCCG ATATCAGAAT 
GAATTAGCTG GCGTAGATAC CGAGCTGCTA GCCGAGAGAT TTTACTACCA AGCCCTGTCA 
GTAGCTCCTC AGATTGGAAT GCCCTTCAAT CAGCTGGGCA CCCTGGCAGG CAGCAAGTAC 
TATAATGTGG AAGCCATGTA TTGCTACCTG CGCTGCATCC AGTCAGAAGT GTCCTTTGAG 
GGAGCCTATG GGAACCTCAA GCGGCTGTAT GACAAGGCAG CCAAAATGTA CCACCAACTG 
AAGAAGTGTG AGACTCGGAA ACTGTCTCCT GGCAAAAAGC GATGTAAAGA CATTAAAAGG 
TTGCTAGTGA ACTTTATGTA TCTGCAAAGC CTCCTACAGC CCAAAAGCAG CTCCGTGGAC 
TCAGAGCTGA CCTCACTTTG CCAGTCAGTC CTGGAGGACT TCAACCTCTG CCTCTTCTAC 
CTGCCCTCCT CACCCAACCT CAGCCTGGCC AGTGAGGATG AGGAGGAGTA TGAGAGTGGA 
TATGCTTTCC TCCCGGACCT TCTCATCTTT CAAATGGTCA TCATCTGCCT TATGTGTGTG 
CACAGCTTGG AGAGAGCAGG ATCCAAGCAG TACAGTGCAG CCATTGCCTT CACCCTGGCC 
CTCTTTTCCC ACCTCGTCAA TCATGTCAAC ATACGGCTGC AGGCTGAGCT GGAAGAGGGC 
GAGAATCCCG TCCCGGCATT CCAGAGTGAT GGCACAGATG AACCAGAGTC CAAGGAACCT 
GTGGAGAAAG AGGAGGAGCC AGATCCTGAG CCTCCTCCTG TAACACCCCA AGTGGGTGAG 
GGCAGAAAGA GCCGTAAGTT CTCTCGCCTC TCCTGTCTCC GCCGTCGCCG CCACCCACCC 
AAAGTTGGTG ATGACAGTGA CCTGAGTGAA GGCTTTGAAT CGGACTCAAG CCATGACTCA 
GCCCGGGCCA GTGAGGGCTC AGACAGTGGC TCTGACAAGA GTCTTGAAGG TGGGGGAACG 
GCCTTTGATG CTGAAACAGA CTCGGAAATG AATAGCCAGG AGTCCCGATC AGACTTGGAA 
GATATGGAGG AAGAGGAGGG GACACGGTCA CCAACCCTGG AGCCCCCTCG GGGCAGATCA 
GAGGCTCCCG ATTCCCTCAA TGGCCCACTG GGCCCCAGTG AGGCTAGCAT TGCCAGCAAT 
CTACAAGCCA TGTCCACCCA GATGTTCCAG ACTAAGCGCT GCTTCCGACT GGCCCCCACC 
TTTAGCAACC TGCTCCTCCA GCCCACCACC AACCCTCATA CCTCGGCCAG CCACAGGCCT 
TGCGTCAATG GGGATGTAGA CAAGCCTTCA GAGCCAGCCT CTGAGGAGGG CTCTGAGTCG 
GAGGGGAGTG AGTCCAGTGG ACGCTCCTGT CGGAATGAGC GCAGCATCCA GGAGAAGCTT 
CAGGTCCTGA TGGCCGAAGG TCTGCTTCCT GCTGTGAAAG TCTTCCTGGA CTGGCTTCGG 
ACCAACCCCG ACCTCATCAT CGTGTGTGCG CAGAGCTCTC AAAGTCTGTG GAACCGCCTG 
TCTGTGTTGC TGAATCTGTT GCCTGCTGCT GGTGAACTCC AGGAGTCTGG CCTGGCCTTG 
TGTCCTGAGG TCCAAGATCT TCTTGAAGGT TGTGAACTGC CTGACCTCCC CTCTAGCCTT 
CTGCTCCCAG AGGACATGGC TCTTCGTAAC CTGCCCCCGC TCCGAGCTGC CCACAGACGC 
TTTAACTTTG ACACGGATCG GCCCCTGCTC AGCACCTTAG AGGAGTCAGT GGTGCGCATC 
TGCTGCATCC GCAGCTTTGG TCATTTCATC GCCCGCCTGC AAGGCAGCAT CCTGCAGTTC 
AACCCAGAGG TTGGCATCTT CGTCAGCATT GCCCAGTCTG AGCAGGAGAG CCTGCTGCAG 
CAGGCCCAGG CACAGTTCCG AATGGCACAG GAGGAAGCTC GTCGGAACAG GCTCATGAGA 
GACATGGCTC AGCTACGACT TCAGCTCGAA GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG 
CCCAAGGCCC AGTCAGCCAT GTCTCCCTAC CTCGTCCCTG ACACCCAGGC CCTCTGCCAC 
CATCTCCCTG TCATCCGCCA ACTGGCCACC AGTGGCCGCT TCATTGTCAT CATCCCAAGG 
ACAGTGATCG ATGGCCTGGA TTTGCTGAAG AAGGAACACC CAGGGGCCCG GGATGGGATT 
CGGTACCTGG AGGCAGAGTT TAAAAAAGGA AACAGGTACA TTCGCTGCCA GAAAGAGGTG 
GGAAAGAGCT TTGAGCGGCA TAAGCTGAAG AGGCAGGATG CAGATGCCTG GACTCTCTAT 
AAGATCCTAG ACAGCTGCAA ACAGCTGACT CTGGCCCAGG GGGCAGGTGA GGAGGATCCG 
AGTGGCATGG TGACCATCAT CACAGGCCTT CCACTGGACA ACCCCAGCGT GCTTTCAGGC 
CCCATGCAGG CAGCCCTGCA GGCCGCTGCC CACGCCAGTG TGGACATCAA GAATGTTCTG 
GACTTCTACA AGCAGTGGAA GGAAATTGGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310545.1
CDS	541..3393
Translation	MFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	NM_001323616.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323616.1

ORF Insert Sequence:

ATGTTCCTGC ACCCAGTGGA CTATGGGAGA AAGGCTGAGG AGCTGCTGTG GAGAAAGGTA 
TACTATGAAG TTATCCAGCT TATCAAGACT AACAAAAAGC ACATCCACAG CCGGAGCACT 
TTGGAATGTG CCTACAGGAC GCACCTGGTT GCTGGTATTG GCTTCTACCA GCATCTCCTT 
CTCTATATCC AGTCCCACTA CCAGCTGGAA CTGCAGTGCT GCATCGACTG GACCCATGTC 
ACTGACCCCC TCATAGGATG CAAGAAGCCA GTGTCTGCCT CAGGGAAGGA GATGGATTGG 
GCACAGATGG CATGTCACCG ATGTCTGGTG TATCTGGGGG ATTTGTCCCG ATATCAGAAT 
GAATTAGCTG GCGTAGATAC CGAGCTGCTA GCCGAGAGAT TTTACTACCA AGCCCTGTCA 
GTAGCTCCTC AGATTGGAAT GCCCTTCAAT CAGCTGGGCA CCCTGGCAGG CAGCAAGTAC 
TATAATGTGG AAGCCATGTA TTGCTACCTG CGCTGCATCC AGTCAGAAGT GTCCTTTGAG 
GGAGCCTATG GGAACCTCAA GCGGCTGTAT GACAAGGCAG CCAAAATGTA CCACCAACTG 
AAGAAGTGTG AGACTCGGAA ACTGTCTCCT GGCAAAAAGC GATGTAAAGA CATTAAAAGG 
TTGCTAGTGA ACTTTATGTA TCTGCAAAGC CTCCTACAGC CCAAAAGCAG CTCCGTGGAC 
TCAGAGCTGA CCTCACTTTG CCAGTCAGTC CTGGAGGACT TCAACCTCTG CCTCTTCTAC 
CTGCCCTCCT CACCCAACCT CAGCCTGGCC AGTGAGGATG AGGAGGAGTA TGAGAGTGGA 
TATGCTTTCC TCCCGGACCT TCTCATCTTT CAAATGGTCA TCATCTGCCT TATGTGTGTG 
CACAGCTTGG AGAGAGCAGG ATCCAAGCAG TACAGTGCAG CCATTGCCTT CACCCTGGCC 
CTCTTTTCCC ACCTCGTCAA TCATGTCAAC ATACGGCTGC AGGCTGAGCT GGAAGAGGGC 
GAGAATCCCG TCCCGGCATT CCAGAGTGAT GGCACAGATG AACCAGAGTC CAAGGAACCT 
GTGGAGAAAG AGGAGGAGCC AGATCCTGAG CCTCCTCCTG TAACACCCCA AGTGGGTGAG 
GGCAGAAAGA GCCGTAAGTT CTCTCGCCTC TCCTGTCTCC GCCGTCGCCG CCACCCACCC 
AAAGTTGGTG ATGACAGTGA CCTGAGTGAA GGCTTTGAAT CGGACTCAAG CCATGACTCA 
GCCCGGGCCA GTGAGGGCTC AGACAGTGGC TCTGACAAGA GTCTTGAAGG TGGGGGAACG 
GCCTTTGATG CTGAAACAGA CTCGGAAATG AATAGCCAGG AGTCCCGATC AGACTTGGAA 
GATATGGAGG AAGAGGAGGG GACACGGTCA CCAACCCTGG AGCCCCCTCG GGGCAGATCA 
GAGGCTCCCG ATTCCCTCAA TGGCCCACTG GGCCCCAGTG AGGCTAGCAT TGCCAGCAAT 
CTACAAGCCA TGTCCACCCA GATGTTCCAG ACTAAGCGCT GCTTCCGACT GGCCCCCACC 
TTTAGCAACC TGCTCCTCCA GCCCACCACC AACCCTCATA CCTCGGCCAG CCACAGGCCT 
TGCGTCAATG GGGATGTAGA CAAGCCTTCA GAGCCAGCCT CTGAGGAGGG CTCTGAGTCG 
GAGGGGAGTG AGTCCAGTGG ACGCTCCTGT CGGAATGAGC GCAGCATCCA GGAGAAGCTT 
CAGGTCCTGA TGGCCGAAGG TCTGCTTCCT GCTGTGAAAG TCTTCCTGGA CTGGCTTCGG 
ACCAACCCCG ACCTCATCAT CGTGTGTGCG CAGAGCTCTC AAAGTCTGTG GAACCGCCTG 
TCTGTGTTGC TGAATCTGTT GCCTGCTGCT GGTGAACTCC AGGAGTCTGG CCTGGCCTTG 
TGTCCTGAGG TCCAAGATCT TCTTGAAGGT TGTGAACTGC CTGACCTCCC CTCTAGCCTT 
CTGCTCCCAG AGGACATGGC TCTTCGTAAC CTGCCCCCGC TCCGAGCTGC CCACAGACGC 
TTTAACTTTG ACACGGATCG GCCCCTGCTC AGCACCTTAG AGGAGTCAGT GGTGCGCATC 
TGCTGCATCC GCAGCTTTGG TCATTTCATC GCCCGCCTGC AAGGCAGCAT CCTGCAGTTC 
AACCCAGAGG TTGGCATCTT CGTCAGCATT GCCCAGTCTG AGCAGGAGAG CCTGCTGCAG 
CAGGCCCAGG CACAGTTCCG AATGGCACAG GAGGAAGCTC GTCGGAACAG GCTCATGAGA 
GACATGGCTC AGCTACGACT TCAGCTCGAA GTGTCTCAGC TGGAGGGCAG CCTGCAGCAG 
CCCAAGGCCC AGTCAGCCAT GTCTCCCTAC CTCGTCCCTG ACACCCAGGC CCTCTGCCAC 
CATCTCCCTG TCATCCGCCA ACTGGCCACC AGTGGCCGCT TCATTGTCAT CATCCCAAGG 
ACAGTGATCG ATGGCCTGGA TTTGCTGAAG AAGGAACACC CAGGGGCCCG GGATGGGATT 
CGGTACCTGG AGGCAGAGTT TAAAAAAGGA AACAGGTACA TTCGCTGCCA GAAAGAGGTG 
GGAAAGAGCT TTGAGCGGCA TAAGCTGAAG AGGCAGGATG CAGATGCCTG GACTCTCTAT 
AAGATCCTAG ACAGCTGCAA ACAGCTGACT CTGGCCCAGG GGGCAGGTGA GGAGGATCCG 
AGTGGCATGG TGACCATCAT CACAGGCCTT CCACTGGACA ACCCCAGCGT GCTTTCAGGC 
CCCATGCAGG CAGCCCTGCA GGCCGCTGCC CACGCCAGTG TGGACATCAA GAATGTTCTG 
GACTTCTACA AGCAGTGGAA GGAAATTGGT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( NM_001323614.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310543.1 Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu66116
Clone ID Related Accession (Same CDS sequence)	NM_001323614.1
Accession Version	NM_001323614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	protein SMG5 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL589685.17 and AL135927.14. On Apr 22, 2016 this sequence version replaced XM_011509361.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.133653.1, SRR1803613.226320.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCCG AAGCAAAAGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT ATTCAAACCA GAAAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTGGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTATA CTATGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAC 
ATCCACAGCC GGAGCACTTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCCCACTACC AGCTGGAACT GCAGTGCTGC 
ATCGACTGGA CCCATGTCAC TGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGTCACCGAT GTCTGGTGTA TCTGGGGGAT 
TTGTCCCGAT ATCAGAATGA ATTAGCTGGC GTAGATACCG AGCTGCTAGC CGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAATCA GCTGGGCACC 
CTGGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATCCAG 
TCAGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAAATGTACC ACCAACTGAA GAAGTGTGAG ACTCGGAAAC TGTCTCCTGG CAAAAAGCGA 
TGTAAAGACA TTAAAAGGTT GCTAGTGAAC TTTATGTATC TGCAAAGCCT CCTACAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACC TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGTGTGTGCA CAGCTTGGAG AGAGCAGGAT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTTTCCCAC CTCGTCAATC ATGTCAACAT ACGGCTGCAG 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCATTCC AGAGTGATGG CACAGATGAA 
CCAGAGTCCA AGGAACCTGT GGAGAAAGAG GAGGAGCCAG ATCCTGAGCC TCCTCCTGTA 
ACACCCCAAG TGGGTGAGGG CAGAAAGAGC CGTAAGTTCT CTCGCCTCTC CTGTCTCCGC 
CGTCGCCGCC ACCCACCCAA AGTTGGTGAT GACAGTGACC TGAGTGAAGG CTTTGAATCG 
GACTCAAGCC ATGACTCAGC CCGGGCCAGT GAGGGCTCAG ACAGTGGCTC TGACAAGAGT 
CTTGAAGGTG GGGGAACGGC CTTTGATGCT GAAACAGACT CGGAAATGAA TAGCCAGGAG 
TCCCGATCAG ACTTGGAAGA TATGGAGGAA GAGGAGGGGA CACGGTCACC AACCCTGGAG 
CCCCCTCGGG GCAGATCAGA GGCTCCCGAT TCCCTCAATG GCCCACTGGG CCCCAGTGAG 
GCTAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC TAAGCGCTGC 
TTCCGACTGG CCCCCACCTT TAGCAACCTG CTCCTCCAGC CCACCACCAA CCCTCATACC 
TCGGCCAGCC ACAGGCCTTG CGTCAATGGG GATGTAGACA AGCCTTCAGA GCCAGCCTCT 
GAGGAGGGCT CTGAGTCGGA GGGGAGTGAG TCCAGTGGAC GCTCCTGTCG GAATGAGCGC 
AGCATCCAGG AGAAGCTTCA GGTCCTGATG GCCGAAGGTC TGCTTCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTTCGGAC CAACCCCGAC CTCATCATCG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGCTG AATCTGTTGC CTGCTGCTGG TGAACTCCAG 
GAGTCTGGCC TGGCCTTGTG TCCTGAGGTC CAAGATCTTC TTGAAGGTTG TGAACTGCCT 
GACCTCCCCT CTAGCCTTCT GCTCCCAGAG GACATGGCTC TTCGTAACCT GCCCCCGCTC 
CGAGCTGCCC ACAGACGCTT TAACTTTGAC ACGGATCGGC CCCTGCTCAG CACCTTAGAG 
GAGGCACAGG AGGAAGCTCG TCGGAACAGG CTCATGAGAG ACATGGCTCA GCTACGACTT 
CAGCTCGAAG TGTCTCAGCT GGAGGGCAGC CTGCAGCAGC CCAAGGCCCA GTCAGCCATG 
TCTCCCTACC TCGTCCCTGA CACCCAGGCC CTCTGCCACC ATCTCCCTGT CATCCGCCAA 
CTGGCCACCA GTGGCCGCTT CATTGTCATC ATCCCAAGGA CAGTGATCGA TGGCCTGGAT 
TTGCTGAAGA AGGAACACCC AGGGGCCCGG GATGGGATTC GGTACCTGGA GGCAGAGTTT 
AAAAAAGGAA ACAGGTACAT TCGCTGCCAG AAAGAGGTGG GAAAGAGCTT TGAGCGGCAT 
AAGCTGAAGA GGCAGGATGC AGATGCCTGG ACTCTCTATA AGATCCTAGA CAGCTGCAAA 
CAGCTGACTC TGGCCCAGGG GGCAGGTGAG GAGGATCCGA GTGGCATGGT GACCATCATC 
ACAGGCCTTC CACTGGACAA CCCCAGCGTG CTTTCAGGCC CCATGCAGGC AGCCCTGCAG 
GCCGCTGCCC ACGCCAGTGT GGACATCAAG AATGTTCTGG ACTTCTACAA GCAGTGGAAG 
GAAATTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310543.1
CDS	211..3102
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEEAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	NM_001323614.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001323614.1

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCCG AAGCAAAAGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT ATTCAAACCA GAAAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTGGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTATA CTATGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAC 
ATCCACAGCC GGAGCACTTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCCCACTACC AGCTGGAACT GCAGTGCTGC 
ATCGACTGGA CCCATGTCAC TGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGTCACCGAT GTCTGGTGTA TCTGGGGGAT 
TTGTCCCGAT ATCAGAATGA ATTAGCTGGC GTAGATACCG AGCTGCTAGC CGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAATCA GCTGGGCACC 
CTGGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATCCAG 
TCAGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAAATGTACC ACCAACTGAA GAAGTGTGAG ACTCGGAAAC TGTCTCCTGG CAAAAAGCGA 
TGTAAAGACA TTAAAAGGTT GCTAGTGAAC TTTATGTATC TGCAAAGCCT CCTACAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACC TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGTGTGTGCA CAGCTTGGAG AGAGCAGGAT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTTTCCCAC CTCGTCAATC ATGTCAACAT ACGGCTGCAG 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCATTCC AGAGTGATGG CACAGATGAA 
CCAGAGTCCA AGGAACCTGT GGAGAAAGAG GAGGAGCCAG ATCCTGAGCC TCCTCCTGTA 
ACACCCCAAG TGGGTGAGGG CAGAAAGAGC CGTAAGTTCT CTCGCCTCTC CTGTCTCCGC 
CGTCGCCGCC ACCCACCCAA AGTTGGTGAT GACAGTGACC TGAGTGAAGG CTTTGAATCG 
GACTCAAGCC ATGACTCAGC CCGGGCCAGT GAGGGCTCAG ACAGTGGCTC TGACAAGAGT 
CTTGAAGGTG GGGGAACGGC CTTTGATGCT GAAACAGACT CGGAAATGAA TAGCCAGGAG 
TCCCGATCAG ACTTGGAAGA TATGGAGGAA GAGGAGGGGA CACGGTCACC AACCCTGGAG 
CCCCCTCGGG GCAGATCAGA GGCTCCCGAT TCCCTCAATG GCCCACTGGG CCCCAGTGAG 
GCTAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC TAAGCGCTGC 
TTCCGACTGG CCCCCACCTT TAGCAACCTG CTCCTCCAGC CCACCACCAA CCCTCATACC 
TCGGCCAGCC ACAGGCCTTG CGTCAATGGG GATGTAGACA AGCCTTCAGA GCCAGCCTCT 
GAGGAGGGCT CTGAGTCGGA GGGGAGTGAG TCCAGTGGAC GCTCCTGTCG GAATGAGCGC 
AGCATCCAGG AGAAGCTTCA GGTCCTGATG GCCGAAGGTC TGCTTCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTTCGGAC CAACCCCGAC CTCATCATCG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGCTG AATCTGTTGC CTGCTGCTGG TGAACTCCAG 
GAGTCTGGCC TGGCCTTGTG TCCTGAGGTC CAAGATCTTC TTGAAGGTTG TGAACTGCCT 
GACCTCCCCT CTAGCCTTCT GCTCCCAGAG GACATGGCTC TTCGTAACCT GCCCCCGCTC 
CGAGCTGCCC ACAGACGCTT TAACTTTGAC ACGGATCGGC CCCTGCTCAG CACCTTAGAG 
GAGGCACAGG AGGAAGCTCG TCGGAACAGG CTCATGAGAG ACATGGCTCA GCTACGACTT 
CAGCTCGAAG TGTCTCAGCT GGAGGGCAGC CTGCAGCAGC CCAAGGCCCA GTCAGCCATG 
TCTCCCTACC TCGTCCCTGA CACCCAGGCC CTCTGCCACC ATCTCCCTGT CATCCGCCAA 
CTGGCCACCA GTGGCCGCTT CATTGTCATC ATCCCAAGGA CAGTGATCGA TGGCCTGGAT 
TTGCTGAAGA AGGAACACCC AGGGGCCCGG GATGGGATTC GGTACCTGGA GGCAGAGTTT 
AAAAAAGGAA ACAGGTACAT TCGCTGCCAG AAAGAGGTGG GAAAGAGCTT TGAGCGGCAT 
AAGCTGAAGA GGCAGGATGC AGATGCCTGG ACTCTCTATA AGATCCTAGA CAGCTGCAAA 
CAGCTGACTC TGGCCCAGGG GGCAGGTGAG GAGGATCCGA GTGGCATGGT GACCATCATC 
ACAGGCCTTC CACTGGACAA CCCCAGCGTG CTTTCAGGCC CCATGCAGGC AGCCCTGCAG 
GCCGCTGCCC ACGCCAGTGT GGACATCAAG AATGTTCTGG ACTTCTACAA GCAGTGGAAG 
GAAATTGGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_017000842.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856331.1 Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

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CloneID	OHu87131
Clone ID Related Accession (Same CDS sequence)	XM_017000842.1
Accession Version	XM_017000842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCA ATCAGCTGGG CACCCTGGCA GGCAGCAAGT ACTATAATGT GGAAGCCATG 
TATTGCTACC TGCGCTGCAT CCAGTCAGAA GTGTCCTTTG AGGGAGCCTA TGGGAACCTC 
AAGCGGCTGT ATGACAAGGC AGCCAAAATG TACCACCAAC TGAAGAAGTG TGAGACTCGG 
AAACTGTCTC CTGGCAAAAA GCGATGTAAA GACATTAAAA GGTTGCTAGT GAACTTTATG 
TATCTGCAAA GCCTCCTACA GCCCAAAAGC AGCTCCGTGG ACTCAGAGCT GACCTCACTT 
TGCCAGTCAG TCCTGGAGGA CTTCAACCTC TGCCTCTTCT ACCTGCCCTC CTCACCCAAC 
CTCAGCCTGG CCAGTGAGGA TGAGGAGGAG TATGAGAGTG GATATGCTTT CCTCCCGGAC 
CTTCTCATCT TTCAAATGGT CATCATCTGC CTTATGTGTG TGCACAGCTT GGAGAGAGCA 
GGATCCAAGC AGTACAGTGC AGCCATTGCC TTCACCCTGG CCCTCTTTTC CCACCTCGTC 
AATCATGTCA ACATACGGCT GCAGGCTGAG CTGGAAGAGG GCGAGAATCC CGTCCCGGCA 
TTCCAGAGTG ATGGCACAGA TGAACCAGAG TCCAAGGAAC CTGTGGAGAA AGAGGAGGAG 
CCAGATCCTG AGCCTCCTCC TGTAACACCC CAAGTGGGTG AGGGCAGAAA GAGCCGTAAG 
TTCTCTCGCC TCTCCTGTCT CCGCCGTCGC CGCCACCCAC CCAAAGTTGG TGATGACAGT 
GACCTGAGTG AAGGCTTTGA ATCGGACTCA AGCCATGACT CAGCCCGGGC CAGTGAGGGC 
TCAGACAGTG GCTCTGACAA GAGTCTTGAA GGTGGGGGAA CGGCCTTTGA TGCTGAAACA 
GACTCGGAAA TGAATAGCCA GGAGTCCCGA TCAGACTTGG AAGATATGGA GGAAGAGGAG 
GGGACACGGT CACCAACCCT GGAGCCCCCT CGGGGCAGAT CAGAGGCTCC CGATTCCCTC 
AATGGCCCAC TGGGCCCCAG TGAGGCTAGC ATTGCCAGCA ATCTACAAGC CATGTCCACC 
CAGATGTTCC AGACTAAGCG CTGCTTCCGA CTGGCCCCCA CCTTTAGCAA CCTGCTCCTC 
CAGCCCACCA CCAACCCTCA TACCTCGGCC AGCCACAGGC CTTGCGTCAA TGGGGATGTA 
GACAAGCCTT CAGAGCCAGC CTCTGAGGAG GGCTCTGAGT CGGAGGGGAG TGAGTCCAGT 
GGACGCTCCT GTCGGAATGA GCGCAGCATC CAGGAGAAGC TTCAGGTCCT GATGGCCGAA 
GGTCTGCTTC CTGCTGTGAA AGTCTTCCTG GACTGGCTTC GGACCAACCC CGACCTCATC 
ATCGTGTGTG CGCAGAGCTC TCAAAGTCTG TGGAACCGCC TGTCTGTGTT GCTGAATCTG 
TTGCCTGCTG CTGGTGAACT CCAGGAGTCT GGCCTGGCCT TGTGTCCTGA GGTCCAAGAT 
CTTCTTGAAG GTTGTGAACT GCCTGACCTC CCCTCTAGCC TTCTGCTCCC AGAGGACATG 
GCTCTTCGTA ACCTGCCCCC GCTCCGAGCT GCCCACAGAC GCTTTAACTT TGACACGGAT 
CGGCCCCTGC TCAGCACCTT AGAGGAGTCA GTGGTGCGCA TCTGCTGCAT CCGCAGCTTT 
GGTCATTTCA TCGCCCGCCT GCAAGGCAGC ATCCTGCAGT TCAACCCAGA GGTTGGCATC 
TTCGTCAGCA TTGCCCAGTC TGAGCAGGAG AGCCTGCTGC AGCAGGCCCA GGCACAGTTC 
CGAATGGCAC AGGAGGAAGC TCGTCGGAAC AGGCTCATGA GAGACATGGC TCAGCTACGA 
CTTCAGCTCG AAGTGTCTCA GCTGGAGGGC AGCCTGCAGC AGCCCAAGGC CCAGTCAGCC 
ATGTCTCCCT ACCTCGTCCC TGACACCCAG GCCCTCTGCC ACCATCTCCC TGTCATCCGC 
CAACTGGCCA CCAGTGGCCG CTTCATTGTC ATCATCCCAA GGACAGTGAT CGATGGCCTG 
GATTTGCTGA AGAAGGAACA CCCAGGGGCC CGGGATGGGA TTCGGTACCT GGAGGCAGAG 
TTTAAAAAAG GAAACAGGTA CATTCGCTGC CAGAAAGAGG TGGGAAAGAG CTTTGAGCGG 
CATAAGCTGA AGAGGCAGGA TGCAGATGCC TGGACTCTCT ATAAGATCCT AGACAGCTGC 
AAACAGCTGA CTCTGGCCCA GGGGGCAGGT GAGGAGGATC CGAGTGGCAT GGTGACCATC 
ATCACAGGCC TTCCACTGGA CAACCCCAGC GTGCTTTCAG GCCCCATGCA GGCAGCCCTG 
CAGGCCGCTG CCCACGCCAG TGTGGACATC AAGAATGTTC TGGACTTCTA CAAGCAGTGG 
AAGGAAATTG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856331.1
CDS	732..3146
Translation	MPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_017000842.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000842.1

ORF Insert Sequence:

ATGCCCTTCA ATCAGCTGGG CACCCTGGCA GGCAGCAAGT ACTATAATGT GGAAGCCATG 
TATTGCTACC TGCGCTGCAT CCAGTCAGAA GTGTCCTTTG AGGGAGCCTA TGGGAACCTC 
AAGCGGCTGT ATGACAAGGC AGCCAAAATG TACCACCAAC TGAAGAAGTG TGAGACTCGG 
AAACTGTCTC CTGGCAAAAA GCGATGTAAA GACATTAAAA GGTTGCTAGT GAACTTTATG 
TATCTGCAAA GCCTCCTACA GCCCAAAAGC AGCTCCGTGG ACTCAGAGCT GACCTCACTT 
TGCCAGTCAG TCCTGGAGGA CTTCAACCTC TGCCTCTTCT ACCTGCCCTC CTCACCCAAC 
CTCAGCCTGG CCAGTGAGGA TGAGGAGGAG TATGAGAGTG GATATGCTTT CCTCCCGGAC 
CTTCTCATCT TTCAAATGGT CATCATCTGC CTTATGTGTG TGCACAGCTT GGAGAGAGCA 
GGATCCAAGC AGTACAGTGC AGCCATTGCC TTCACCCTGG CCCTCTTTTC CCACCTCGTC 
AATCATGTCA ACATACGGCT GCAGGCTGAG CTGGAAGAGG GCGAGAATCC CGTCCCGGCA 
TTCCAGAGTG ATGGCACAGA TGAACCAGAG TCCAAGGAAC CTGTGGAGAA AGAGGAGGAG 
CCAGATCCTG AGCCTCCTCC TGTAACACCC CAAGTGGGTG AGGGCAGAAA GAGCCGTAAG 
TTCTCTCGCC TCTCCTGTCT CCGCCGTCGC CGCCACCCAC CCAAAGTTGG TGATGACAGT 
GACCTGAGTG AAGGCTTTGA ATCGGACTCA AGCCATGACT CAGCCCGGGC CAGTGAGGGC 
TCAGACAGTG GCTCTGACAA GAGTCTTGAA GGTGGGGGAA CGGCCTTTGA TGCTGAAACA 
GACTCGGAAA TGAATAGCCA GGAGTCCCGA TCAGACTTGG AAGATATGGA GGAAGAGGAG 
GGGACACGGT CACCAACCCT GGAGCCCCCT CGGGGCAGAT CAGAGGCTCC CGATTCCCTC 
AATGGCCCAC TGGGCCCCAG TGAGGCTAGC ATTGCCAGCA ATCTACAAGC CATGTCCACC 
CAGATGTTCC AGACTAAGCG CTGCTTCCGA CTGGCCCCCA CCTTTAGCAA CCTGCTCCTC 
CAGCCCACCA CCAACCCTCA TACCTCGGCC AGCCACAGGC CTTGCGTCAA TGGGGATGTA 
GACAAGCCTT CAGAGCCAGC CTCTGAGGAG GGCTCTGAGT CGGAGGGGAG TGAGTCCAGT 
GGACGCTCCT GTCGGAATGA GCGCAGCATC CAGGAGAAGC TTCAGGTCCT GATGGCCGAA 
GGTCTGCTTC CTGCTGTGAA AGTCTTCCTG GACTGGCTTC GGACCAACCC CGACCTCATC 
ATCGTGTGTG CGCAGAGCTC TCAAAGTCTG TGGAACCGCC TGTCTGTGTT GCTGAATCTG 
TTGCCTGCTG CTGGTGAACT CCAGGAGTCT GGCCTGGCCT TGTGTCCTGA GGTCCAAGAT 
CTTCTTGAAG GTTGTGAACT GCCTGACCTC CCCTCTAGCC TTCTGCTCCC AGAGGACATG 
GCTCTTCGTA ACCTGCCCCC GCTCCGAGCT GCCCACAGAC GCTTTAACTT TGACACGGAT 
CGGCCCCTGC TCAGCACCTT AGAGGAGTCA GTGGTGCGCA TCTGCTGCAT CCGCAGCTTT 
GGTCATTTCA TCGCCCGCCT GCAAGGCAGC ATCCTGCAGT TCAACCCAGA GGTTGGCATC 
TTCGTCAGCA TTGCCCAGTC TGAGCAGGAG AGCCTGCTGC AGCAGGCCCA GGCACAGTTC 
CGAATGGCAC AGGAGGAAGC TCGTCGGAAC AGGCTCATGA GAGACATGGC TCAGCTACGA 
CTTCAGCTCG AAGTGTCTCA GCTGGAGGGC AGCCTGCAGC AGCCCAAGGC CCAGTCAGCC 
ATGTCTCCCT ACCTCGTCCC TGACACCCAG GCCCTCTGCC ACCATCTCCC TGTCATCCGC 
CAACTGGCCA CCAGTGGCCG CTTCATTGTC ATCATCCCAA GGACAGTGAT CGATGGCCTG 
GATTTGCTGA AGAAGGAACA CCCAGGGGCC CGGGATGGGA TTCGGTACCT GGAGGCAGAG 
TTTAAAAAAG GAAACAGGTA CATTCGCTGC CAGAAAGAGG TGGGAAAGAG CTTTGAGCGG 
CATAAGCTGA AGAGGCAGGA TGCAGATGCC TGGACTCTCT ATAAGATCCT AGACAGCTGC 
AAACAGCTGA CTCTGGCCCA GGGGGCAGGT GAGGAGGATC CGAGTGGCAT GGTGACCATC 
ATCACAGGCC TTCCACTGGA CAACCCCAGC GTGCTTTCAG GCCCCATGCA GGCAGCCCTG 
CAGGCCGCTG CCCACGCCAG TGTGGACATC AAGAATGTTC TGGACTTCTA CAAGCAGTGG 
AAGGAAATTG GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( XM_017000843.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856332.1 Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu87132
Clone ID Related Accession (Same CDS sequence)	XM_017000843.1
Accession Version	XM_017000843.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein SMG5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCACC AACTGAAGAA GTGTGAGACT CGGAAACTGT CTCCTGGCAA AAAGCGATGT 
AAAGACATTA AAAGGTTGCT AGTGAACTTT ATGTATCTGC AAAGCCTCCT ACAGCCCAAA 
AGCAGCTCCG TGGACTCAGA GCTGACCTCA CTTTGCCAGT CAGTCCTGGA GGACTTCAAC 
CTCTGCCTCT TCTACCTGCC CTCCTCACCC AACCTCAGCC TGGCCAGTGA GGATGAGGAG 
GAGTATGAGA GTGGATATGC TTTCCTCCCG GACCTTCTCA TCTTTCAAAT GGTCATCATC 
TGCCTTATGT GTGTGCACAG CTTGGAGAGA GCAGGATCCA AGCAGTACAG TGCAGCCATT 
GCCTTCACCC TGGCCCTCTT TTCCCACCTC GTCAATCATG TCAACATACG GCTGCAGGCT 
GAGCTGGAAG AGGGCGAGAA TCCCGTCCCG GCATTCCAGA GTGATGGCAC AGATGAACCA 
GAGTCCAAGG AACCTGTGGA GAAAGAGGAG GAGCCAGATC CTGAGCCTCC TCCTGTAACA 
CCCCAAGTGG GTGAGGGCAG AAAGAGCCGT AAGTTCTCTC GCCTCTCCTG TCTCCGCCGT 
CGCCGCCACC CACCCAAAGT TGGTGATGAC AGTGACCTGA GTGAAGGCTT TGAATCGGAC 
TCAAGCCATG ACTCAGCCCG GGCCAGTGAG GGCTCAGACA GTGGCTCTGA CAAGAGTCTT 
GAAGGTGGGG GAACGGCCTT TGATGCTGAA ACAGACTCGG AAATGAATAG CCAGGAGTCC 
CGATCAGACT TGGAAGATAT GGAGGAAGAG GAGGGGACAC GGTCACCAAC CCTGGAGCCC 
CCTCGGGGCA GATCAGAGGC TCCCGATTCC CTCAATGGCC CACTGGGCCC CAGTGAGGCT 
AGCATTGCCA GCAATCTACA AGCCATGTCC ACCCAGATGT TCCAGACTAA GCGCTGCTTC 
CGACTGGCCC CCACCTTTAG CAACCTGCTC CTCCAGCCCA CCACCAACCC TCATACCTCG 
GCCAGCCACA GGCCTTGCGT CAATGGGGAT GTAGACAAGC CTTCAGAGCC AGCCTCTGAG 
GAGGGCTCTG AGTCGGAGGG GAGTGAGTCC AGTGGACGCT CCTGTCGGAA TGAGCGCAGC 
ATCCAGGAGA AGCTTCAGGT CCTGATGGCC GAAGGTCTGC TTCCTGCTGT GAAAGTCTTC 
CTGGACTGGC TTCGGACCAA CCCCGACCTC ATCATCGTGT GTGCGCAGAG CTCTCAAAGT 
CTGTGGAACC GCCTGTCTGT GTTGCTGAAT CTGTTGCCTG CTGCTGGTGA ACTCCAGGAG 
TCTGGCCTGG CCTTGTGTCC TGAGGTCCAA GATCTTCTTG AAGGTTGTGA ACTGCCTGAC 
CTCCCCTCTA GCCTTCTGCT CCCAGAGGAC ATGGCTCTTC GTAACCTGCC CCCGCTCCGA 
GCTGCCCACA GACGCTTTAA CTTTGACACG GATCGGCCCC TGCTCAGCAC CTTAGAGGAG 
TCAGTGGTGC GCATCTGCTG CATCCGCAGC TTTGGTCATT TCATCGCCCG CCTGCAAGGC 
AGCATCCTGC AGTTCAACCC AGAGGTTGGC ATCTTCGTCA GCATTGCCCA GTCTGAGCAG 
GAGAGCCTGC TGCAGCAGGC CCAGGCACAG TTCCGAATGG CACAGGAGGA AGCTCGTCGG 
AACAGGCTCA TGAGAGACAT GGCTCAGCTA CGACTTCAGC TCGAAGTGTC TCAGCTGGAG 
GGCAGCCTGC AGCAGCCCAA GGCCCAGTCA GCCATGTCTC CCTACCTCGT CCCTGACACC 
CAGGCCCTCT GCCACCATCT CCCTGTCATC CGCCAACTGG CCACCAGTGG CCGCTTCATT 
GTCATCATCC CAAGGACAGT GATCGATGGC CTGGATTTGC TGAAGAAGGA ACACCCAGGG 
GCCCGGGATG GGATTCGGTA CCTGGAGGCA GAGTTTAAAA AAGGAAACAG GTACATTCGC 
TGCCAGAAAG AGGTGGGAAA GAGCTTTGAG CGGCATAAGC TGAAGAGGCA GGATGCAGAT 
GCCTGGACTC TCTATAAGAT CCTAGACAGC TGCAAACAGC TGACTCTGGC CCAGGGGGCA 
GGTGAGGAGG ATCCGAGTGG CATGGTGACC ATCATCACAG GCCTTCCACT GGACAACCCC 
AGCGTGCTTT CAGGCCCCAT GCAGGCAGCC CTGCAGGCCG CTGCCCACGC CAGTGTGGAC 
ATCAAGAATG TTCTGGACTT CTACAAGCAG TGGAAGGAAA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856332.1
CDS	861..3128
Translation	MYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	XM_017000843.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000843.1

ORF Insert Sequence:

ATGTACCACC AACTGAAGAA GTGTGAGACT CGGAAACTGT CTCCTGGCAA AAAGCGATGT 
AAAGACATTA AAAGGTTGCT AGTGAACTTT ATGTATCTGC AAAGCCTCCT ACAGCCCAAA 
AGCAGCTCCG TGGACTCAGA GCTGACCTCA CTTTGCCAGT CAGTCCTGGA GGACTTCAAC 
CTCTGCCTCT TCTACCTGCC CTCCTCACCC AACCTCAGCC TGGCCAGTGA GGATGAGGAG 
GAGTATGAGA GTGGATATGC TTTCCTCCCG GACCTTCTCA TCTTTCAAAT GGTCATCATC 
TGCCTTATGT GTGTGCACAG CTTGGAGAGA GCAGGATCCA AGCAGTACAG TGCAGCCATT 
GCCTTCACCC TGGCCCTCTT TTCCCACCTC GTCAATCATG TCAACATACG GCTGCAGGCT 
GAGCTGGAAG AGGGCGAGAA TCCCGTCCCG GCATTCCAGA GTGATGGCAC AGATGAACCA 
GAGTCCAAGG AACCTGTGGA GAAAGAGGAG GAGCCAGATC CTGAGCCTCC TCCTGTAACA 
CCCCAAGTGG GTGAGGGCAG AAAGAGCCGT AAGTTCTCTC GCCTCTCCTG TCTCCGCCGT 
CGCCGCCACC CACCCAAAGT TGGTGATGAC AGTGACCTGA GTGAAGGCTT TGAATCGGAC 
TCAAGCCATG ACTCAGCCCG GGCCAGTGAG GGCTCAGACA GTGGCTCTGA CAAGAGTCTT 
GAAGGTGGGG GAACGGCCTT TGATGCTGAA ACAGACTCGG AAATGAATAG CCAGGAGTCC 
CGATCAGACT TGGAAGATAT GGAGGAAGAG GAGGGGACAC GGTCACCAAC CCTGGAGCCC 
CCTCGGGGCA GATCAGAGGC TCCCGATTCC CTCAATGGCC CACTGGGCCC CAGTGAGGCT 
AGCATTGCCA GCAATCTACA AGCCATGTCC ACCCAGATGT TCCAGACTAA GCGCTGCTTC 
CGACTGGCCC CCACCTTTAG CAACCTGCTC CTCCAGCCCA CCACCAACCC TCATACCTCG 
GCCAGCCACA GGCCTTGCGT CAATGGGGAT GTAGACAAGC CTTCAGAGCC AGCCTCTGAG 
GAGGGCTCTG AGTCGGAGGG GAGTGAGTCC AGTGGACGCT CCTGTCGGAA TGAGCGCAGC 
ATCCAGGAGA AGCTTCAGGT CCTGATGGCC GAAGGTCTGC TTCCTGCTGT GAAAGTCTTC 
CTGGACTGGC TTCGGACCAA CCCCGACCTC ATCATCGTGT GTGCGCAGAG CTCTCAAAGT 
CTGTGGAACC GCCTGTCTGT GTTGCTGAAT CTGTTGCCTG CTGCTGGTGA ACTCCAGGAG 
TCTGGCCTGG CCTTGTGTCC TGAGGTCCAA GATCTTCTTG AAGGTTGTGA ACTGCCTGAC 
CTCCCCTCTA GCCTTCTGCT CCCAGAGGAC ATGGCTCTTC GTAACCTGCC CCCGCTCCGA 
GCTGCCCACA GACGCTTTAA CTTTGACACG GATCGGCCCC TGCTCAGCAC CTTAGAGGAG 
TCAGTGGTGC GCATCTGCTG CATCCGCAGC TTTGGTCATT TCATCGCCCG CCTGCAAGGC 
AGCATCCTGC AGTTCAACCC AGAGGTTGGC ATCTTCGTCA GCATTGCCCA GTCTGAGCAG 
GAGAGCCTGC TGCAGCAGGC CCAGGCACAG TTCCGAATGG CACAGGAGGA AGCTCGTCGG 
AACAGGCTCA TGAGAGACAT GGCTCAGCTA CGACTTCAGC TCGAAGTGTC TCAGCTGGAG 
GGCAGCCTGC AGCAGCCCAA GGCCCAGTCA GCCATGTCTC CCTACCTCGT CCCTGACACC 
CAGGCCCTCT GCCACCATCT CCCTGTCATC CGCCAACTGG CCACCAGTGG CCGCTTCATT 
GTCATCATCC CAAGGACAGT GATCGATGGC CTGGATTTGC TGAAGAAGGA ACACCCAGGG 
GCCCGGGATG GGATTCGGTA CCTGGAGGCA GAGTTTAAAA AAGGAAACAG GTACATTCGC 
TGCCAGAAAG AGGTGGGAAA GAGCTTTGAG CGGCATAAGC TGAAGAGGCA GGATGCAGAT 
GCCTGGACTC TCTATAAGAT CCTAGACAGC TGCAAACAGC TGACTCTGGC CCAGGGGGCA 
GGTGAGGAGG ATCCGAGTGG CATGGTGACC ATCATCACAG GCCTTCCACT GGACAACCCC 
AGCGTGCTTT CAGGCCCCAT GCAGGCAGCC CTGCAGGCCG CTGCCCACGC CAGTGTGGAC 
ATCAAGAATG TTCTGGACTT CTACAAGCAG TGGAAGGAAA TTGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( NM_015327.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056142.2 Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15477
Clone ID Related Accession (Same CDS sequence)	NM_015327.2 , NM_015327.3
Accession Version	NM_015327.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	protein SMG5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT000143.1, AB029012.2 and BC038296.1. On May 13, 2008 this sequence version replaced NM_015327.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038296.1, SRR1803613.61129.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCCG AAGCAAAAGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT ATTCAAACCA GAAAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTGGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTATA CTATGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAC 
ATCCACAGCC GGAGCACTTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCCCACTACC AGCTGGAACT GCAGTGCTGC 
ATCGACTGGA CCCATGTCAC TGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGTCACCGAT GTCTGGTGTA TCTGGGGGAT 
TTGTCCCGAT ATCAGAATGA ATTAGCTGGC GTAGATACCG AGCTGCTAGC CGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAATCA GCTGGGCACC 
CTGGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATCCAG 
TCAGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAAATGTACC ACCAACTGAA GAAGTGTGAG ACTCGGAAAC TGTCTCCTGG CAAAAAGCGA 
TGTAAAGACA TTAAAAGGTT GCTAGTGAAC TTTATGTATC TGCAAAGCCT CCTACAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACC TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGTGTGTGCA CAGCTTGGAG AGAGCAGGAT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTTTCCCAC CTCGTCAATC ATGTCAACAT ACGGCTGCAG 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCATTCC AGAGTGATGG CACAGATGAA 
CCAGAGTCCA AGGAACCTGT GGAGAAAGAG GAGGAGCCAG ATCCTGAGCC TCCTCCTGTA 
ACACCCCAAG TGGGTGAGGG CAGAAAGAGC CGTAAGTTCT CTCGCCTCTC CTGTCTCCGC 
CGTCGCCGCC ACCCACCCAA AGTTGGTGAT GACAGTGACC TGAGTGAAGG CTTTGAATCG 
GACTCAAGCC ATGACTCAGC CCGGGCCAGT GAGGGCTCAG ACAGTGGCTC TGACAAGAGT 
CTTGAAGGTG GGGGAACGGC CTTTGATGCT GAAACAGACT CGGAAATGAA TAGCCAGGAG 
TCCCGATCAG ACTTGGAAGA TATGGAGGAA GAGGAGGGGA CACGGTCACC AACCCTGGAG 
CCCCCTCGGG GCAGATCAGA GGCTCCCGAT TCCCTCAATG GCCCACTGGG CCCCAGTGAG 
GCTAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC TAAGCGCTGC 
TTCCGACTGG CCCCCACCTT TAGCAACCTG CTCCTCCAGC CCACCACCAA CCCTCATACC 
TCGGCCAGCC ACAGGCCTTG CGTCAATGGG GATGTAGACA AGCCTTCAGA GCCAGCCTCT 
GAGGAGGGCT CTGAGTCGGA GGGGAGTGAG TCCAGTGGAC GCTCCTGTCG GAATGAGCGC 
AGCATCCAGG AGAAGCTTCA GGTCCTGATG GCCGAAGGTC TGCTTCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTTCGGAC CAACCCCGAC CTCATCATCG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGCTG AATCTGTTGC CTGCTGCTGG TGAACTCCAG 
GAGTCTGGCC TGGCCTTGTG TCCTGAGGTC CAAGATCTTC TTGAAGGTTG TGAACTGCCT 
GACCTCCCCT CTAGCCTTCT GCTCCCAGAG GACATGGCTC TTCGTAACCT GCCCCCGCTC 
CGAGCTGCCC ACAGACGCTT TAACTTTGAC ACGGATCGGC CCCTGCTCAG CACCTTAGAG 
GAGTCAGTGG TGCGCATCTG CTGCATCCGC AGCTTTGGTC ATTTCATCGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA CCCAGAGGTT GGCATCTTCG TCAGCATTGC CCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCA CAGTTCCGAA TGGCACAGGA GGAAGCTCGT 
CGGAACAGGC TCATGAGAGA CATGGCTCAG CTACGACTTC AGCTCGAAGT GTCTCAGCTG 
GAGGGCAGCC TGCAGCAGCC CAAGGCCCAG TCAGCCATGT CTCCCTACCT CGTCCCTGAC 
ACCCAGGCCC TCTGCCACCA TCTCCCTGTC ATCCGCCAAC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATTCG GTACCTGGAG GCAGAGTTTA AAAAAGGAAA CAGGTACATT 
CGCTGCCAGA AAGAGGTGGG AAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCA 
GATGCCTGGA CTCTCTATAA GATCCTAGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGG 
GCAGGTGAGG AGGATCCGAG TGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAC 
CCCAGCGTGC TTTCAGGCCC CATGCAGGCA GCCCTGCAGG CCGCTGCCCA CGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AAATTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056142.2
CDS	150..3200
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	NM_015327.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG5, nonsense mediated mRNA decay factor (SMG5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015327.2

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCCG AAGCAAAAGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT ATTCAAACCA GAAAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTGGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTATA CTATGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAC 
ATCCACAGCC GGAGCACTTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCCCACTACC AGCTGGAACT GCAGTGCTGC 
ATCGACTGGA CCCATGTCAC TGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGTCACCGAT GTCTGGTGTA TCTGGGGGAT 
TTGTCCCGAT ATCAGAATGA ATTAGCTGGC GTAGATACCG AGCTGCTAGC CGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAATCA GCTGGGCACC 
CTGGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATCCAG 
TCAGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAAATGTACC ACCAACTGAA GAAGTGTGAG ACTCGGAAAC TGTCTCCTGG CAAAAAGCGA 
TGTAAAGACA TTAAAAGGTT GCTAGTGAAC TTTATGTATC TGCAAAGCCT CCTACAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACC TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGTGTGTGCA CAGCTTGGAG AGAGCAGGAT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTTTCCCAC CTCGTCAATC ATGTCAACAT ACGGCTGCAG 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCATTCC AGAGTGATGG CACAGATGAA 
CCAGAGTCCA AGGAACCTGT GGAGAAAGAG GAGGAGCCAG ATCCTGAGCC TCCTCCTGTA 
ACACCCCAAG TGGGTGAGGG CAGAAAGAGC CGTAAGTTCT CTCGCCTCTC CTGTCTCCGC 
CGTCGCCGCC ACCCACCCAA AGTTGGTGAT GACAGTGACC TGAGTGAAGG CTTTGAATCG 
GACTCAAGCC ATGACTCAGC CCGGGCCAGT GAGGGCTCAG ACAGTGGCTC TGACAAGAGT 
CTTGAAGGTG GGGGAACGGC CTTTGATGCT GAAACAGACT CGGAAATGAA TAGCCAGGAG 
TCCCGATCAG ACTTGGAAGA TATGGAGGAA GAGGAGGGGA CACGGTCACC AACCCTGGAG 
CCCCCTCGGG GCAGATCAGA GGCTCCCGAT TCCCTCAATG GCCCACTGGG CCCCAGTGAG 
GCTAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC TAAGCGCTGC 
TTCCGACTGG CCCCCACCTT TAGCAACCTG CTCCTCCAGC CCACCACCAA CCCTCATACC 
TCGGCCAGCC ACAGGCCTTG CGTCAATGGG GATGTAGACA AGCCTTCAGA GCCAGCCTCT 
GAGGAGGGCT CTGAGTCGGA GGGGAGTGAG TCCAGTGGAC GCTCCTGTCG GAATGAGCGC 
AGCATCCAGG AGAAGCTTCA GGTCCTGATG GCCGAAGGTC TGCTTCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTTCGGAC CAACCCCGAC CTCATCATCG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGCTG AATCTGTTGC CTGCTGCTGG TGAACTCCAG 
GAGTCTGGCC TGGCCTTGTG TCCTGAGGTC CAAGATCTTC TTGAAGGTTG TGAACTGCCT 
GACCTCCCCT CTAGCCTTCT GCTCCCAGAG GACATGGCTC TTCGTAACCT GCCCCCGCTC 
CGAGCTGCCC ACAGACGCTT TAACTTTGAC ACGGATCGGC CCCTGCTCAG CACCTTAGAG 
GAGTCAGTGG TGCGCATCTG CTGCATCCGC AGCTTTGGTC ATTTCATCGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA CCCAGAGGTT GGCATCTTCG TCAGCATTGC CCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCA CAGTTCCGAA TGGCACAGGA GGAAGCTCGT 
CGGAACAGGC TCATGAGAGA CATGGCTCAG CTACGACTTC AGCTCGAAGT GTCTCAGCTG 
GAGGGCAGCC TGCAGCAGCC CAAGGCCCAG TCAGCCATGT CTCCCTACCT CGTCCCTGAC 
ACCCAGGCCC TCTGCCACCA TCTCCCTGTC ATCCGCCAAC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATTCG GTACCTGGAG GCAGAGTTTA AAAAAGGAAA CAGGTACATT 
CGCTGCCAGA AAGAGGTGGG AAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCA 
GATGCCTGGA CTCTCTATAA GATCCTAGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGG 
GCAGGTGAGG AGGATCCGAG TGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAC 
CCCAGCGTGC TTTCAGGCCC CATGCAGGCA GCCCTGCAGG CCGCTGCCCA CGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AAATTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMG5 ( NM_015327.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056142.2 Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15477
Clone ID Related Accession (Same CDS sequence)	NM_015327.2 , NM_015327.3
Accession Version	NM_015327.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	protein SMG5 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT000143.1, AB029012.2 and BC038296.1. On Nov 22, 2018 this sequence version replaced NM_015327.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038296.1, SRR1803612.64849.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361813.5/ ENSP00000355261.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCCG AAGCAAAAGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT ATTCAAACCA GAAAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTGGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTATA CTATGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAC 
ATCCACAGCC GGAGCACTTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCCCACTACC AGCTGGAACT GCAGTGCTGC 
ATCGACTGGA CCCATGTCAC TGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGTCACCGAT GTCTGGTGTA TCTGGGGGAT 
TTGTCCCGAT ATCAGAATGA ATTAGCTGGC GTAGATACCG AGCTGCTAGC CGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAATCA GCTGGGCACC 
CTGGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATCCAG 
TCAGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAAATGTACC ACCAACTGAA GAAGTGTGAG ACTCGGAAAC TGTCTCCTGG CAAAAAGCGA 
TGTAAAGACA TTAAAAGGTT GCTAGTGAAC TTTATGTATC TGCAAAGCCT CCTACAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACC TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGTGTGTGCA CAGCTTGGAG AGAGCAGGAT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTTTCCCAC CTCGTCAATC ATGTCAACAT ACGGCTGCAG 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCATTCC AGAGTGATGG CACAGATGAA 
CCAGAGTCCA AGGAACCTGT GGAGAAAGAG GAGGAGCCAG ATCCTGAGCC TCCTCCTGTA 
ACACCCCAAG TGGGTGAGGG CAGAAAGAGC CGTAAGTTCT CTCGCCTCTC CTGTCTCCGC 
CGTCGCCGCC ACCCACCCAA AGTTGGTGAT GACAGTGACC TGAGTGAAGG CTTTGAATCG 
GACTCAAGCC ATGACTCAGC CCGGGCCAGT GAGGGCTCAG ACAGTGGCTC TGACAAGAGT 
CTTGAAGGTG GGGGAACGGC CTTTGATGCT GAAACAGACT CGGAAATGAA TAGCCAGGAG 
TCCCGATCAG ACTTGGAAGA TATGGAGGAA GAGGAGGGGA CACGGTCACC AACCCTGGAG 
CCCCCTCGGG GCAGATCAGA GGCTCCCGAT TCCCTCAATG GCCCACTGGG CCCCAGTGAG 
GCTAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC TAAGCGCTGC 
TTCCGACTGG CCCCCACCTT TAGCAACCTG CTCCTCCAGC CCACCACCAA CCCTCATACC 
TCGGCCAGCC ACAGGCCTTG CGTCAATGGG GATGTAGACA AGCCTTCAGA GCCAGCCTCT 
GAGGAGGGCT CTGAGTCGGA GGGGAGTGAG TCCAGTGGAC GCTCCTGTCG GAATGAGCGC 
AGCATCCAGG AGAAGCTTCA GGTCCTGATG GCCGAAGGTC TGCTTCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTTCGGAC CAACCCCGAC CTCATCATCG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGCTG AATCTGTTGC CTGCTGCTGG TGAACTCCAG 
GAGTCTGGCC TGGCCTTGTG TCCTGAGGTC CAAGATCTTC TTGAAGGTTG TGAACTGCCT 
GACCTCCCCT CTAGCCTTCT GCTCCCAGAG GACATGGCTC TTCGTAACCT GCCCCCGCTC 
CGAGCTGCCC ACAGACGCTT TAACTTTGAC ACGGATCGGC CCCTGCTCAG CACCTTAGAG 
GAGTCAGTGG TGCGCATCTG CTGCATCCGC AGCTTTGGTC ATTTCATCGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA CCCAGAGGTT GGCATCTTCG TCAGCATTGC CCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCA CAGTTCCGAA TGGCACAGGA GGAAGCTCGT 
CGGAACAGGC TCATGAGAGA CATGGCTCAG CTACGACTTC AGCTCGAAGT GTCTCAGCTG 
GAGGGCAGCC TGCAGCAGCC CAAGGCCCAG TCAGCCATGT CTCCCTACCT CGTCCCTGAC 
ACCCAGGCCC TCTGCCACCA TCTCCCTGTC ATCCGCCAAC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATTCG GTACCTGGAG GCAGAGTTTA AAAAAGGAAA CAGGTACATT 
CGCTGCCAGA AAGAGGTGGG AAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCA 
GATGCCTGGA CTCTCTATAA GATCCTAGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGG 
GCAGGTGAGG AGGATCCGAG TGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAC 
CCCAGCGTGC TTTCAGGCCC CATGCAGGCA GCCCTGCAGG CCGCTGCCCA CGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AAATTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056142.2
CDS	146..3196
Translation	MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG

Target ORF information:

RefSeq Version	NM_015327.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SMG5 nonsense mediated mRNA decay factor (SMG5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015327.3

ORF Insert Sequence:

ATGAGCCAAG GCCCCCCCAC AGGGGAGAGC AGCGAGCCCG AAGCAAAAGT CCTCCACACT 
AAGCGGCTTT ACCGGGCTGT GGTGGAGGCT GTGCATCGAC TTGACCTCAT CCTTTGCAAC 
AAAACTGCTT ATCAAGAAGT ATTCAAACCA GAAAACATTA GCCTGAGGAA CAAGCTGCGT 
GAGCTCTGCG TCAAGCTTAT GTTCCTGCAC CCAGTGGACT ATGGGAGAAA GGCTGAGGAG 
CTGCTGTGGA GAAAGGTATA CTATGAAGTT ATCCAGCTTA TCAAGACTAA CAAAAAGCAC 
ATCCACAGCC GGAGCACTTT GGAATGTGCC TACAGGACGC ACCTGGTTGC TGGTATTGGC 
TTCTACCAGC ATCTCCTTCT CTATATCCAG TCCCACTACC AGCTGGAACT GCAGTGCTGC 
ATCGACTGGA CCCATGTCAC TGACCCCCTC ATAGGATGCA AGAAGCCAGT GTCTGCCTCA 
GGGAAGGAGA TGGATTGGGC ACAGATGGCA TGTCACCGAT GTCTGGTGTA TCTGGGGGAT 
TTGTCCCGAT ATCAGAATGA ATTAGCTGGC GTAGATACCG AGCTGCTAGC CGAGAGATTT 
TACTACCAAG CCCTGTCAGT AGCTCCTCAG ATTGGAATGC CCTTCAATCA GCTGGGCACC 
CTGGCAGGCA GCAAGTACTA TAATGTGGAA GCCATGTATT GCTACCTGCG CTGCATCCAG 
TCAGAAGTGT CCTTTGAGGG AGCCTATGGG AACCTCAAGC GGCTGTATGA CAAGGCAGCC 
AAAATGTACC ACCAACTGAA GAAGTGTGAG ACTCGGAAAC TGTCTCCTGG CAAAAAGCGA 
TGTAAAGACA TTAAAAGGTT GCTAGTGAAC TTTATGTATC TGCAAAGCCT CCTACAGCCC 
AAAAGCAGCT CCGTGGACTC AGAGCTGACC TCACTTTGCC AGTCAGTCCT GGAGGACTTC 
AACCTCTGCC TCTTCTACCT GCCCTCCTCA CCCAACCTCA GCCTGGCCAG TGAGGATGAG 
GAGGAGTATG AGAGTGGATA TGCTTTCCTC CCGGACCTTC TCATCTTTCA AATGGTCATC 
ATCTGCCTTA TGTGTGTGCA CAGCTTGGAG AGAGCAGGAT CCAAGCAGTA CAGTGCAGCC 
ATTGCCTTCA CCCTGGCCCT CTTTTCCCAC CTCGTCAATC ATGTCAACAT ACGGCTGCAG 
GCTGAGCTGG AAGAGGGCGA GAATCCCGTC CCGGCATTCC AGAGTGATGG CACAGATGAA 
CCAGAGTCCA AGGAACCTGT GGAGAAAGAG GAGGAGCCAG ATCCTGAGCC TCCTCCTGTA 
ACACCCCAAG TGGGTGAGGG CAGAAAGAGC CGTAAGTTCT CTCGCCTCTC CTGTCTCCGC 
CGTCGCCGCC ACCCACCCAA AGTTGGTGAT GACAGTGACC TGAGTGAAGG CTTTGAATCG 
GACTCAAGCC ATGACTCAGC CCGGGCCAGT GAGGGCTCAG ACAGTGGCTC TGACAAGAGT 
CTTGAAGGTG GGGGAACGGC CTTTGATGCT GAAACAGACT CGGAAATGAA TAGCCAGGAG 
TCCCGATCAG ACTTGGAAGA TATGGAGGAA GAGGAGGGGA CACGGTCACC AACCCTGGAG 
CCCCCTCGGG GCAGATCAGA GGCTCCCGAT TCCCTCAATG GCCCACTGGG CCCCAGTGAG 
GCTAGCATTG CCAGCAATCT ACAAGCCATG TCCACCCAGA TGTTCCAGAC TAAGCGCTGC 
TTCCGACTGG CCCCCACCTT TAGCAACCTG CTCCTCCAGC CCACCACCAA CCCTCATACC 
TCGGCCAGCC ACAGGCCTTG CGTCAATGGG GATGTAGACA AGCCTTCAGA GCCAGCCTCT 
GAGGAGGGCT CTGAGTCGGA GGGGAGTGAG TCCAGTGGAC GCTCCTGTCG GAATGAGCGC 
AGCATCCAGG AGAAGCTTCA GGTCCTGATG GCCGAAGGTC TGCTTCCTGC TGTGAAAGTC 
TTCCTGGACT GGCTTCGGAC CAACCCCGAC CTCATCATCG TGTGTGCGCA GAGCTCTCAA 
AGTCTGTGGA ACCGCCTGTC TGTGTTGCTG AATCTGTTGC CTGCTGCTGG TGAACTCCAG 
GAGTCTGGCC TGGCCTTGTG TCCTGAGGTC CAAGATCTTC TTGAAGGTTG TGAACTGCCT 
GACCTCCCCT CTAGCCTTCT GCTCCCAGAG GACATGGCTC TTCGTAACCT GCCCCCGCTC 
CGAGCTGCCC ACAGACGCTT TAACTTTGAC ACGGATCGGC CCCTGCTCAG CACCTTAGAG 
GAGTCAGTGG TGCGCATCTG CTGCATCCGC AGCTTTGGTC ATTTCATCGC CCGCCTGCAA 
GGCAGCATCC TGCAGTTCAA CCCAGAGGTT GGCATCTTCG TCAGCATTGC CCAGTCTGAG 
CAGGAGAGCC TGCTGCAGCA GGCCCAGGCA CAGTTCCGAA TGGCACAGGA GGAAGCTCGT 
CGGAACAGGC TCATGAGAGA CATGGCTCAG CTACGACTTC AGCTCGAAGT GTCTCAGCTG 
GAGGGCAGCC TGCAGCAGCC CAAGGCCCAG TCAGCCATGT CTCCCTACCT CGTCCCTGAC 
ACCCAGGCCC TCTGCCACCA TCTCCCTGTC ATCCGCCAAC TGGCCACCAG TGGCCGCTTC 
ATTGTCATCA TCCCAAGGAC AGTGATCGAT GGCCTGGATT TGCTGAAGAA GGAACACCCA 
GGGGCCCGGG ATGGGATTCG GTACCTGGAG GCAGAGTTTA AAAAAGGAAA CAGGTACATT 
CGCTGCCAGA AAGAGGTGGG AAAGAGCTTT GAGCGGCATA AGCTGAAGAG GCAGGATGCA 
GATGCCTGGA CTCTCTATAA GATCCTAGAC AGCTGCAAAC AGCTGACTCT GGCCCAGGGG 
GCAGGTGAGG AGGATCCGAG TGGCATGGTG ACCATCATCA CAGGCCTTCC ACTGGACAAC 
CCCAGCGTGC TTTCAGGCCC CATGCAGGCA GCCCTGCAGG CCGCTGCCCA CGCCAGTGTG 
GACATCAAGA ATGTTCTGGA CTTCTACAAG CAGTGGAAGG AAATTGGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dissecting the functions of SMG5, SMG7 and PNRC2 in nonsense-mediated mRNA decay of human cells.
RNA (New York, N.Y.)(2018 Jan)
Nicholson P,Gkratsou A,Josi C,Colombo M,M?hlemann O

MicroRNA 433 regulates nonsense-mediated mRNA decay by targeting SMG5 mRNA.
BMC molecular biology17(1)17(2016 07)
Jin Y,Zhang F,Ma Z,Ren Z

The host nonsense-mediated mRNA decay pathway restricts Mammalian RNA virus replication.
Cell host & microbe16(3)403-11(2014 Sep)
Balistreri G,Horvath P,Schweingruber C,Z?nd D,McInerney G,Merits A,M?hlemann O,Azzalin C,Helenius A

Phospho-dependent and phospho-independent interactions of the helicase UPF1 with the NMD factors SMG5-SMG7 and SMG6.
Nucleic acids research42(14)9447-60(2014 Aug)
Chakrabarti S,Bonneau F,Sch?ssler S,Eppinger E,Conti E

Multiple nonglycemic genomic loci are newly associated with blood level of glycated hemoglobin in East Asians.
Diabetes63(7)2551-62(2014 Jul)
Chen P,Takeuchi F,Lee JY,Li H,Wu JY,Liang J,Long J,Tabara Y,Goodarzi MO,Pereira MA,Kim YJ,Go MJ,Stram DO,Vithana E,Khor CC,Liu J,Liao J,Ye X,Wang Y,Lu L,Young TL,Lee J,Thai AC,Cheng CY,van Dam RM,Friedlander Y,Heng CK,Koh WP,Chen CH,Chang LC,Pan WH,Qi Q,Isono M,Zheng W,Cai Q,Gao Y,Yamamoto K,Ohnaka K,Takayanagi R,Kita Y,Ueshima H,Hsiung CA,Cui J,Sheu WH,Rotter JI,Chen YD,Hsu C,Okada Y,Kubo M,Takahashi A,Tanaka T,van Rooij FJ,Ganesh SK,Huang J,Huang T,Yuan J,Hwang JY,,Gross MD,Assimes TL,Miki T,Shu XO,Qi L,Chen YT,Lin X,Aung T,Wong TY,Teo YY,Kim BJ,Kato N,Tai ES

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SMG5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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