Mid2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mid2
Entrez Gene ID	363502
Full Name	midline 2
Synonyms	RGD1565674
General protein information	Preferred Names midline 2 Names probable E3 ubiquitin-protein ligase MID2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq33

mRNA and Protein(s)

mRNA	Protein	Name
XM_006234247.3	XP_006234309.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_006234248.3	XP_006234310.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
NM_001191889.1	NP_001178818.1	probable E3 ubiquitin-protein ligase MID2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MID2	XP_521213.3
Gallus gallus (chicken)	MID2	XP_004940642.1
Rattus norvegicus (Norway rat)	Mid2	NP_001178818.1
Homo sapiens (human)	MID2	NP_036348.2
Canis lupus familiaris (dog)	MID2	XP_005641704.1
Mus musculus (house mouse)	Mid2	NP_035975.1
Xenopus tropicalis (tropical clawed frog)	LOC100497646	XP_002936823.1

Gene Ontology
Bibliography

Related articles in PubMed

TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity.
Kimura T, Jain A, Choi SW, Mandell MA, Schroder K, Johansen T, Deretic V
The Journal of cell biology210(6)973-89(2015 Sep)

TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.
Uchil PD, Hinz A, Siegel S, Coenen-Stass A, Pertel T, Luban J, Mothes W
Journal of virology87(1)257-72(2013 Jan)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
Uchil PD, Quinlan BD, Chan WT, Luna JM, Mothes W
PLoS pathogens4(2)e16(2008 Feb)

MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders.
Short KM, Hopwood B, Yi Z, Cox TC
BMC cell biology31(2002)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mid2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mid2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07935	NM_001191889.1 Latest version!	Rattus norvegicus midline 2 (Mid2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa25887	XM_006234247.3 Latest version!	Rattus norvegicus midline 2 (Mid2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa25888	XM_006234248.3 Latest version!	Rattus norvegicus midline 2 (Mid2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mid2 ( NM_001191889.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178818.1 Rattus norvegicus midline 2 (Mid2), mRNA.

Price & Availability↑

CloneID	ORa07935
Clone ID Related Accession (Same CDS sequence)	NM_001191889.1
Accession Version	NM_001191889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Rattus norvegicus(Norway rat)
Product	probable E3 ubiquitin-protein ligase MID2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07040845.1, AABR07040846.1 and AABR07040847.1. On Jul 17, 2010 this sequence version replaced XM_343825.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA 
AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA 
AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG 
GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGACTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGACAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTC 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATTGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CAGGCTAAAA ACAAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGACGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACGGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CAATCGGTAT TGCCTACAAA TCAGCTCCAA AGAACGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTCACT TCGTGGTGCG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGCGTCCT CTTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCGTA TGTGTCGGGG ATGAAAGCTT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178818.1
CDS	165..2372
Translation	MGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPGSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSHFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	NM_001191889.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus midline 2 (Mid2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191889.1

ORF Insert Sequence:

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA 
AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA 
AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG 
GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGACTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGACAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTC 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATTGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CAGGCTAAAA ACAAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGACGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACGGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CAATCGGTAT TGCCTACAAA TCAGCTCCAA AGAACGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTCACT TCGTGGTGCG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGCGTCCT CTTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCGTA TGTGTCGGGG ATGAAAGCTT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( XM_006234247.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234309.1 Rattus norvegicus midline 2 (Mid2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25887
Clone ID Related Accession (Same CDS sequence)	XM_006234247.3
Accession Version	XM_006234247.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA 
AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA 
AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG 
GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGACAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTC CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATTGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GTGAACCCAC CAGGCTAAAA 
ACAAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGACGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACGGGGTG TTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CAATCGGTAT TGCCTACAAA 
TCAGCTCCAA AGAACGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTCACT TCGTGGTGCG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGCGTCCT CTTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCGTA TGTGTCGGGG 
ATGAAAGCTT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234309.1
CDS	588..2705
Translation	MGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPGSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSHFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_006234247.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus midline 2 (Mid2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234247.3

ORF Insert Sequence:

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA 
AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA 
AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG 
GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGACAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTC CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATTGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GTGAACCCAC CAGGCTAAAA 
ACAAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGACGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACGGGGTG TTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CAATCGGTAT TGCCTACAAA 
TCAGCTCCAA AGAACGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTCACT TCGTGGTGCG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGCGTCCT CTTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCGTA TGTGTCGGGG 
ATGAAAGCTT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( XM_006234248.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234310.1 Rattus norvegicus midline 2 (Mid2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa25888
Clone ID Related Accession (Same CDS sequence)	XM_006234248.3
Accession Version	XM_006234248.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234248.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA 
AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA 
AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG 
GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGACTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGACAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTC 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATTGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CAGGCTAAAA ACAAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGACGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACGGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CAATCGGTAT TGCCTACAAA TCAGCTCCAA AGAACGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTCACT TCGTGGTGCG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGCGTCCT CTTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCGTA TGTGTCGGGG ATGAAAGCTT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234310.1
CDS	256..2403
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPGSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSHFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_006234248.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus midline 2 (Mid2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234248.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA 
AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA 
AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA 
AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG 
GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGACTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGACAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTC 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATTGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GTGAACCCAC CAGGCTAAAA ACAAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGACGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACGGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CAATCGGTAT TGCCTACAAA TCAGCTCCAA AGAACGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTCACT TCGTGGTGCG ACATAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGCGTCCT CTTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCGTA TGTGTCGGGG ATGAAAGCTT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity.
The Journal of cell biology210(6)973-89(2015 Sep)
Kimura T,Jain A,Choi SW,Mandell MA,Schroder K,Johansen T,Deretic V

TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.
Journal of virology87(1)257-72(2013 Jan)
Uchil PD,Hinz A,Siegel S,Coenen-Stass A,Pertel T,Luban J,Mothes W

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

TRIM E3 ligases interfere with early and late stages of the retroviral life cycle.
PLoS pathogens4(2)e16(2008 Feb)
Uchil PD,Quinlan BD,Chan WT,Luna JM,Mothes W

MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders.
BMC cell biology31(2002)
Short KM,Hopwood B,Yi Z,Cox TC