Mid2 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006528524.4	XP_006528587.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
NM_001358367.1	NP_001345296.1	probable E3 ubiquitin-protein ligase MID2 isoform 1
XM_006528523.3	XP_006528586.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
NM_001358366.1	NP_001345295.1	probable E3 ubiquitin-protein ligase MID2 isoform 2
NM_011845.3	NP_035975.1	probable E3 ubiquitin-protein ligase MID2 isoform 3
XM_017318482.1	XP_017173971.1	probable E3 ubiquitin-protein ligase MID2 isoform X3
NM_011845.2	NP_035975.1	probable E3 ubiquitin-protein ligase MID2
XM_006528522.3	XP_006528585.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_006528524.3	XP_006528587.1	probable E3 ubiquitin-protein ligase MID2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu08971	NM_011845.3 Latest version!	Mus musculus midline 2 (Mid2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu56503	NM_001358367.1 Latest version!	Mus musculus midline 2 (Mid2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu56504	NM_001358366.1 Latest version!	Mus musculus midline 2 (Mid2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu08971	XM_017318482.1 Latest version!	Mus musculus midline 2 (Mid2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu56503	XM_006528522.3 Latest version!	Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu56504	XM_006528523.3 Latest version!	Mus musculus midline 2 (Mid2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu56505	XM_006528524.4 Latest version!	Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu08971	NM_011845.2	Mus musculus midline 2 (Mid2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu56505	XM_006528524.3	Mus musculus midline 2 (Mid2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

Mid2 ( NM_011845.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035975.1 Mus musculus midline 2 (Mid2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu08971
Clone ID Related Accession (Same CDS sequence)	NM_011845.3 , XM_017318482.1 , NM_011845.2
Accession Version	NM_011845.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase MID2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL683809.7, AF196480.1 and BX470203.6. On or before Dec 1, 2017 this sequence version replaced XM_017318482.1, NM_011845.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660811.231121.1, SRR1660821.60243.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035975.1
CDS	188..2245
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	NM_011845.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus midline 2 (Mid2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011845.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( NM_011845.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035975.1 Mus musculus midline 2 (Mid2), mRNA.

Price & Availability↑

CloneID	OMu08971
Clone ID Related Accession (Same CDS sequence)	NM_011845.3 , XM_017318482.1 , NM_011845.2
Accession Version	NM_011845.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-28
Organism	Mus musculus(House mouse)
Product	probable E3 ubiquitin-protein ligase MID2
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF196480.1 and BX470203.6. On Mar 22, 2007 this sequence version replaced NM_011845.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by mRNA and EST alignments. ##Evidence-Data-START## Transcript exon combination :: AF196480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035975.1
CDS	25..2082
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	NM_011845.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus midline 2 (Mid2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011845.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( NM_001358367.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345296.1 Mus musculus midline 2 (Mid2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu56503
Clone ID Related Accession (Same CDS sequence)	NM_001358367.1 , XM_006528522.3
Accession Version	NM_001358367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase MID2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL683809.7 and BX470203.6. On Dec 1, 2017 this sequence version replaced XM_006528522.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345296.1
CDS	320..2527
Translation	MGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	NM_001358367.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus midline 2 (Mid2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358367.1

ORF Insert Sequence:

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( NM_001358366.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001345295.1 Mus musculus midline 2 (Mid2), transcript variant 2, mRNA.

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CloneID	OMu56504
Clone ID Related Accession (Same CDS sequence)	XM_006528523.3 , NM_001358366.1
Accession Version	NM_001358366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase MID2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL683809.7 and BX470203.6. On Dec 1, 2017 this sequence version replaced XM_006528523.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: Y18881.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001345295.1
CDS	320..2437
Translation	MGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	NM_001358366.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus midline 2 (Mid2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001358366.1

ORF Insert Sequence:

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( XM_017318482.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173971.1 Mus musculus midline 2 (Mid2), transcript variant X3, mRNA.

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CloneID	OMu08971
Clone ID Related Accession (Same CDS sequence)	NM_011845.3 , XM_017318482.1 , NM_011845.2
Accession Version	XM_017318482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173971.1
CDS	396..2453
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_017318482.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus midline 2 (Mid2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318482.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( XM_006528522.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528585.1 Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.

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CloneID	OMu56503
Clone ID Related Accession (Same CDS sequence)	NM_001358367.1 , XM_006528522.3
Accession Version	XM_006528522.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528522.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528585.1
CDS	602..2809
Translation	MGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_006528522.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528522.3

ORF Insert Sequence:

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( XM_006528523.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528586.1 Mus musculus midline 2 (Mid2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu56504
Clone ID Related Accession (Same CDS sequence)	XM_006528523.3 , NM_001358366.1
Accession Version	XM_006528523.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(House mouse)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528586.1
CDS	600..2717
Translation	MGESPASAVLNASAGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_006528523.3
Organism	Mus musculus(House mouse)
Definition	Mus musculus midline 2 (Mid2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528523.3

ORF Insert Sequence:

ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGATAGCTG GATGATTGTG 
CCCAACATTA AGCAGAACCA TTACACAGTT CATGGACTCC AGAGTGGGAC ACGCTACATC 
TTTATCGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GCGAACCCAC CAGACTAAAA 
ACGAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG 
AGATTTAGTG GCACAGGGTG TTATGGGGCA GCAGGAAATA TATTCATCGA CAGTGGCTGC 
CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CTATTGGTAT TGCCTACAAA 
TCAGCTCCAA AGAATGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC 
AACAGTAACT TCGTGGTTCG GCACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG 
TTGAAGCGTC TAGGTGTCCT TCTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA 
TTCACAATCT GGAACAAATC CCTAATGATT CTTTCTGGCT TGCCTGCCCC TGATTTTATT 
GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTGTCAGGG 
ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( XM_006528524.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528587.1 Mus musculus midline 2 (Mid2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu56505
Clone ID Related Accession (Same CDS sequence)	XM_006528524.4 , XM_006528524.3
Accession Version	XM_006528524.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006528524.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528587.1
CDS	252..2399
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_006528524.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus midline 2 (Mid2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528524.3

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mid2 ( XM_006528524.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006528587.1 Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.

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CloneID	OMu56505
Clone ID Related Accession (Same CDS sequence)	XM_006528524.4 , XM_006528524.3
Accession Version	XM_006528524.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006528524.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528587.1
CDS	396..2543
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPSSAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVSDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPQLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKACH

Target ORF information:

RefSeq Version	XM_006528524.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus midline 2 (Mid2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528524.4

ORF Insert Sequence:

ATGGAAACAC TGGAGTCAGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
CTTCTGCTTC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CCCACCGCAT CTTGGTCTCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACATGCAGG 
TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC 
CGCCGGGAGA GGACTTACAG GCCTAGCTCC GCCATGTCGA GTGAGAGAAT TGCATGTCAA 
TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACGT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC CAGCCATCGC 
CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGCCTGGA CCACGAGAAT 
GAGAAGGTGA ACATGTACTG TGTATCTGAT GATCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG 
CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGTGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA GCGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTA 
AAGGAAAATG ACCAAGCACG GTTTCTGCAG TCTGCAAAAA ACATTGCCGA GAGGGTCGCT 
ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA 
GCTCCAAACC CACCATCTAT CCGAGAAGAG CTCTGTACTG CTTCCCATGA CACCATTACA 
GTCCACTGGA TCTCAGACGA CGAATTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGTGCGCC ACGAGGCCTG 
TACAATTCAG TGGATAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTT 
CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATCGTGA AAGCCATAAA TCAAGCAGGC 
AGCCGGAACA GCGAACCCAC CAGACTAAAA ACGAACAGCC AACCATTTAA ACTAGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAG 
AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACAGGGTG TTATGGGGCA 
GCAGGAAATA TATTCATCGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA 
ACATGGTATG CTATTGGTAT TGCCTACAAA TCAGCTCCAA AGAATGAGTG GATTGGCAAG 
AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTAACT TCGTGGTTCG GCACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGTGTCCT TCTGGATTAT 
GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATT 
CTTTCTGGCT TGCCTGCCCC TGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTGTCAGGG ATGAAAGCTT GCCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Mid2
Entrez Gene ID	23947
Full Name	midline 2
Synonyms	FXY2,Trim1
General protein information	Preferred Names midline 2 Names probable E3 ubiquitin-protein ligase MID2 RING-type E3 ubiquitin transferase MID2 midline defect 2 tripartite motif-containing protein 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X F1\|X 61.35 cM

Pan troglodytes (chimpanzee)	MID2	XP_521213.3
Gallus gallus (chicken)	MID2	XP_004940642.1
Rattus norvegicus (Norway rat)	Mid2	NP_001178818.1
Homo sapiens (human)	MID2	NP_036348.2
Canis lupus familiaris (dog)	MID2	XP_005641704.1
Mus musculus (house mouse)	Mid2	NP_035975.1
Xenopus tropicalis (tropical clawed frog)	LOC100497646	XP_002936823.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Mid2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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