Ufl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ufl1
Entrez Gene ID	313115
Full Name	Ufm1-specific ligase 1
Synonyms	Maxer,RGD1309308
General protein information	Preferred Names Ufm1-specific ligase 1 Names E3 UFM1-protein ligase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_006237959.3	XP_006238021.1	E3 UFM1-protein ligase 1 isoform X1
NM_001126279.1	NP_001119751.1	E3 UFM1-protein ligase 1

Pathways from BioSystems

Homologies

	UFL1	XP_001100312.1
Canis lupus familiaris (dog)	UFL1	XP_532236.4
Bos taurus (cattle)	UFL1	NP_001073745.1
Anopheles gambiae (African malaria mosquito)	UFL1_ANOGA	XP_317778.3
Arabidopsis thaliana (thale cress)	AT3G46220	NP_566883.4
Homo sapiens (human)	UFL1	NP_056138.1
Gallus gallus (chicken)	UFL1	NP_001096671.1
Mus musculus (house mouse)	Ufl1	NP_080470.2
Rattus norvegicus (Norway rat)	Ufl1	NP_001119751.1
Drosophila melanogaster (fruit fly)	CG1104	NP_001246957.1
Oryza sativa (rice)	Os05g0117600	NP_001174184.1
Xenopus tropicalis (tropical clawed frog)	ufl1	XP_002934919.2
Pan troglodytes (chimpanzee)	UFL1	XP_518643.2
Danio rerio (zebrafish)	ufl1	NP_956900.1

Gene Ontology
Bibliography

Related articles in PubMed

Modification of ASC1 by UFM1 is crucial for ER? transactivation and breast cancer development.
Yoo HM, Kang SH, Kim JY, Lee JE, Seong MW, Lee SW, Ka SH, Sou YS, Komatsu M, Tanaka K, Lee ST, Noh DY, Baek SH, Jeon YJ, Chung CH
Molecular cell56(2)261-74(2014 Oct)

Transcriptional regulation of the Ufm1 conjugation system in response to disturbance of the endoplasmic reticulum homeostasis and inhibition of vesicle trafficking.
Zhang Y, Zhang M, Wu J, Lei G, Li H
PloS one7(11)e48587(2012)

Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis.
Lemaire K, Moura RF, Granvik M, Igoillo-Esteve M, Hohmeier HE, Hendrickx N, Newgard CB, Waelkens E, Cnop M, Schuit F
PloS one6(4)e18517(2011 Apr)

Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity.
Shiwaku H, Yoshimura N, Tamura T, Sone M, Ogishima S, Watase K, Tagawa K, Okazawa H
The EMBO journal29(14)2446-60(2010 Jul)

A novel C53/LZAP-interacting protein regulates stability of C53/LZAP and DDRGK domain-containing Protein 1 (DDRGK1) and modulates NF-kappaB signaling.
Wu J, Lei G, Mei M, Tang Y, Li H
The Journal of biological chemistry285(20)15126-36(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ufl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ufl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12568	NM_001126279.1 Latest version!	Rattus norvegicus Ufm1-specific ligase 1 (Ufl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa28080	XM_006237959.3 Latest version!	Rattus norvegicus Ufm1-specific ligase 1 (Ufl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ufl1 ( NM_001126279.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001119751.1 Rattus norvegicus Ufm1-specific ligase 1 (Ufl1), mRNA.

Price & Availability↑

CloneID	ORa12568
Clone ID Related Accession (Same CDS sequence)	NM_001126279.1
Accession Version	NM_001126279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-08
Organism	Rattus norvegicus(Norway rat)
Product	E3 UFM1-protein ligase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC166498.1. On or before May 7, 2008 this sequence version replaced XM_232850.4, XM_001061009.1. ##Evidence-Data-START## Transcript exon combination :: BC166498.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGACG CCTGGGAGGA GATCCGGCGT CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCTGAGTCCA CGCAAAGGCT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTTCATACG CTTGATGGGA AGGAATATAT CACTCCAGCC 
CAGATCAGTA AAGAAATGAG AGATGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTC 
GACTTACAAC AGGTTATTAA CGTGGACCTG ACTCATATTG AAAATAGAGT CGGCGACATT 
ATTAAGTCGG AGAAGCATGT GCAGATGGTA CTGGGACAGC TGGTCGATGA GAACTATCTG 
GATCGGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACCGTCTCA 
GAGCTGTGTA AAACCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTTGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTGG CTCGCCATAA AGCACGCATC CGTGGACTCT TCAGTGCTAT TACCCGGCCT 
ACAGCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTTGAGGAAC TTGTTAGCAC TGGCCGCCTA CGAGGCACGG TGGTTGGCGG GCGACAGGAC 
AAAGCAGTGT TTGTCCCTGA CATCTATTCC AGGACACAGA GTACTTGGGT GGATTCCTTC 
TTCAGGCAGA ACGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCCACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GGCTCGTGGA CCAGGTGGAA GCATCGGTAG AGGAGGCCAT CAGCTCAGGA 
ACATGGGTTG ACGTTTCGCC TCTGCTGCCC AGTTCTTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTTCAGC AGGTGATGAG ACCATTTGGC AAGCATGCGT CAGCCACAGT CTTTAGTGAC 
ACTGTTGTGG TCAGCGAGAA ATTCATCAAT GACTGTACAA AACTGTTCAG CGAACGAATG 
CACCAAAAAG CTGAAAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC AGAAGAAGAT 
CTGAAACAAA TATCCATTTT AGAAAGTGTT AATACAAATA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGTTCTGGA AGTGTAAGAG GCGGAGGAGG GGGCAATGCC 
AGAGAATACA AGATTAAGAA AGTCAAGAAG AAGGGAAGAA AAGACGAGGA CAGTGATGAT 
GAGTCTCAGT CATCGCATGC TGGAAAGAAG AAGCCAGACA TCACCTTCAT GTTCCAGGAT 
GAAATTGAAG GTTGCTTAAG GAAACACATT CCAGATGCCC CTGAGGAGTT TATTTCAGAA 
CTTGCAGAAC ACTTAATAAA GCCTCTGAAT AAAATGTATC TTGAGGTGGT ACGTTCAGTC 
TTCATGTCGT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACGATCAA GGACTTACAA 
GAAGAGGTTT CAAATCTATA CAATAATATC AGATTATTTG AAAAGGGGAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTGACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GACTTGATGA TGGCGGTGGA GGACCCTGCA 
ACCATTACAA GTGATATGAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACTAAAGTA 
GCTCTCACAA AACTCCACAA CTCCCTGAAT GAAAAAAGCA TAGAAGACTT CCTTTCTTGT 
CTGGATTCTG CAACAGAAGC TTGTGACATT ATGGTGAAAA AGGGAGACAA AAAAAGGGAA 
AGGCAAATCC TTTTCCAGCA TCGGCAGGCT CTGGCTGACC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACATCT CACATCAGTC CTGTTATTTC AGTTCTCAAC CCACAGCATG 
CTCCATGCAC CTGGAAGATG TGTCCCACAG ATCATCGCCT TTCTTCACAA CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGATTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGAGTGGACA GGGAGAGGAT CCGTCAAGCG ATGACTTAGA CAAGGAACAA 
CATGACGTCA CCAATACTAC TCGGAAGGAG CTTCAGGAAC TGTCTTTATC CATTAAGGAC 
CTTGTTCTGA AGCCCAGGAA ATCATCTGTG ACAGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119751.1
CDS	48..2429
Translation	MADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDELHVRGGRVNIVDLQQVINVDLTHIENRVGDIIKSEKHVQMVLGQLVDENYLDRLSEEVNDKLQESGQVTVSELCKTYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLVSKYGFQEQLLYSVLEELVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTPLLFLKATCVGQGLVDQVEASVEEAISSGTWVDVSPLLPSSLSVEDAAMLLQQVMRPFGKHASATVFSDTVVVSEKFINDCTKLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTNKKDKKDERRKKATEGSGSVRGGGGGNAREYKIKKVKKKGRKDEDSDDESQSSHAGKKKPDITFMFQDEIEGCLRKHIPDAPEEFISELAEHLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTALTKHLLKTVCTDITNLMFNFLASDLMMAVEDPATITSDMRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALADQLKVTEDPALILHLTSVLLFQFSTHSMLHAPGRCVPQIIAFLHNKIPEDQHTLLVKYQGLVVKQLVSQNKKSGQGEDPSSDDLDKEQHDVTNTTRKELQELSLSIKDLVLKPRKSSVTEE

Target ORF information:

RefSeq Version	NM_001126279.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ufm1-specific ligase 1 (Ufl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126279.1

ORF Insert Sequence:

ATGGCGGACG CCTGGGAGGA GATCCGGCGT CTGGCGGCTG ACTTCCAGCG GGCGCAGTTT 
GCTGAGTCCA CGCAAAGGCT GTCAGAGCGG AACTGCATTG AGATTGTGAA TAAATTGATC 
TCTCAGAAAC AGCTGGAAGT AGTTCATACG CTTGATGGGA AGGAATATAT CACTCCAGCC 
CAGATCAGTA AAGAAATGAG AGATGAGCTA CATGTCCGAG GTGGTCGAGT AAACATTGTC 
GACTTACAAC AGGTTATTAA CGTGGACCTG ACTCATATTG AAAATAGAGT CGGCGACATT 
ATTAAGTCGG AGAAGCATGT GCAGATGGTA CTGGGACAGC TGGTCGATGA GAACTATCTG 
GATCGGTTAT CAGAAGAGGT GAATGATAAA TTGCAAGAAA GTGGTCAAGT AACCGTCTCA 
GAGCTGTGTA AAACCTATGA CCTTCCTGGG GATTTTCTGA CACAGGCATT AACTCAGCGA 
CTTGGCCGGA TTATCAATGG CCATCTTGAC CTTGATAACA GAGGAGTGAT TTTCACTGAG 
GCCTTTGTGG CTCGCCATAA AGCACGCATC CGTGGACTCT TCAGTGCTAT TACCCGGCCT 
ACAGCAGTGA ACTCTTTGGT TTCAAAATAT GGATTTCAGG AACAGCTCCT TTACTCTGTG 
CTTGAGGAAC TTGTTAGCAC TGGCCGCCTA CGAGGCACGG TGGTTGGCGG GCGACAGGAC 
AAAGCAGTGT TTGTCCCTGA CATCTATTCC AGGACACAGA GTACTTGGGT GGATTCCTTC 
TTCAGGCAGA ACGGCTATCT AGAATTTGAC GCTTTATCCA GACTTGGAAT CCCAGATGCT 
GTGAACTATA TAAAGAAAAG GTATAAGAAT ACCCCACTCT TGTTTTTGAA GGCAACTTGT 
GTTGGCCAAG GGCTCGTGGA CCAGGTGGAA GCATCGGTAG AGGAGGCCAT CAGCTCAGGA 
ACATGGGTTG ACGTTTCGCC TCTGCTGCCC AGTTCTTTGT CAGTTGAAGA TGCTGCTATG 
CTGCTTCAGC AGGTGATGAG ACCATTTGGC AAGCATGCGT CAGCCACAGT CTTTAGTGAC 
ACTGTTGTGG TCAGCGAGAA ATTCATCAAT GACTGTACAA AACTGTTCAG CGAACGAATG 
CACCAAAAAG CTGAAAAGGA AATGAAAAAT AATCCTGTGC ATTTAATCAC AGAAGAAGAT 
CTGAAACAAA TATCCATTTT AGAAAGTGTT AATACAAATA AAAAGGATAA AAAAGATGAA 
CGCAGGAAGA AAGCAACAGA GGGTTCTGGA AGTGTAAGAG GCGGAGGAGG GGGCAATGCC 
AGAGAATACA AGATTAAGAA AGTCAAGAAG AAGGGAAGAA AAGACGAGGA CAGTGATGAT 
GAGTCTCAGT CATCGCATGC TGGAAAGAAG AAGCCAGACA TCACCTTCAT GTTCCAGGAT 
GAAATTGAAG GTTGCTTAAG GAAACACATT CCAGATGCCC CTGAGGAGTT TATTTCAGAA 
CTTGCAGAAC ACTTAATAAA GCCTCTGAAT AAAATGTATC TTGAGGTGGT ACGTTCAGTC 
TTCATGTCGT CAACATCTGC TTCTGGAACT GGCAGAAAAC GCACGATCAA GGACTTACAA 
GAAGAGGTTT CAAATCTATA CAATAATATC AGATTATTTG AAAAGGGGAT GAAATATTTT 
GCAGATGACA CACAGACTGC TCTGACCAAG CACTTGCTGA AGACAGTGTG TACCGATATC 
ACTAACCTCA TGTTCAACTT CCTAGCCTCA GACTTGATGA TGGCGGTGGA GGACCCTGCA 
ACCATTACAA GTGATATGAG AAAAAAAATT TTAAGCAAAT TGACAGAAGA AACTAAAGTA 
GCTCTCACAA AACTCCACAA CTCCCTGAAT GAAAAAAGCA TAGAAGACTT CCTTTCTTGT 
CTGGATTCTG CAACAGAAGC TTGTGACATT ATGGTGAAAA AGGGAGACAA AAAAAGGGAA 
AGGCAAATCC TTTTCCAGCA TCGGCAGGCT CTGGCTGACC AGCTGAAGGT CACAGAAGAC 
CCTGCTCTGA TTTTACATCT CACATCAGTC CTGTTATTTC AGTTCTCAAC CCACAGCATG 
CTCCATGCAC CTGGAAGATG TGTCCCACAG ATCATCGCCT TTCTTCACAA CAAAATCCCA 
GAGGATCAAC ATACTCTCTT GGTAAAATAT CAAGGATTGG TTGTGAAACA GTTAGTCAGT 
CAAAATAAGA AGAGTGGACA GGGAGAGGAT CCGTCAAGCG ATGACTTAGA CAAGGAACAA 
CATGACGTCA CCAATACTAC TCGGAAGGAG CTTCAGGAAC TGTCTTTATC CATTAAGGAC 
CTTGTTCTGA AGCCCAGGAA ATCATCTGTG ACAGAGGAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ufl1 ( XM_006237959.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006238021.1 Rattus norvegicus Ufm1-specific ligase 1 (Ufl1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28080
Clone ID Related Accession (Same CDS sequence)	XM_006237959.3
Accession Version	XM_006237959.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2187bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 UFM1-protein ligase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237959.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAGATG AGCTACATGT CCGAGGTGGT CGAGTAAACA TTGTCGACTT ACAACAGGTT 
ATTAACGTGG ACCTGACTCA TATTGAAAAT AGAGTCGGCG ACATTATTAA GTCGGAGAAG 
CATGTGCAGA TGGTACTGGG ACAGCTGGTC GATGAGAACT ATCTGGATCG GTTATCAGAA 
GAGGTGAATG ATAAATTGCA AGAAAGTGGT CAAGTAACCG TCTCAGAGCT GTGTAAAACC 
TATGACCTTC CTGGGGATTT TCTGACACAG GCATTAACTC AGCGACTTGG CCGGATTATC 
AATGGCCATC TTGACCTTGA TAACAGAGGA GTGATTTTCA CTGAGGCCTT TGTGGCTCGC 
CATAAAGCAC GCATCCGTGG ACTCTTCAGT GCTATTACCC GGCCTACAGC AGTGAACTCT 
TTGGTTTCAA AATATGGATT TCAGGAACAG CTCCTTTACT CTGTGCTTGA GGAACTTGTT 
AGCACTGGCC GCCTACGAGG CACGGTGGTT GGCGGGCGAC AGGACAAAGC AGTGTTTGTC 
CCTGACATCT ATTCCAGGAC ACAGAGTACT TGGGTGGATT CCTTCTTCAG GCAGAACGGC 
TATCTAGAAT TTGACGCTTT ATCCAGACTT GGAATCCCAG ATGCTGTGAA CTATATAAAG 
AAAAGGTATA AGAATACCCC ACTCTTGTTT TTGAAGGCAA CTTGTGTTGG CCAAGGGCTC 
GTGGACCAGG TGGAAGCATC GGTAGAGGAG GCCATCAGCT CAGGAACATG GGTTGACGTT 
TCGCCTCTGC TGCCCAGTTC TTTGTCAGTT GAAGATGCTG CTATGCTGCT TCAGCAGGTG 
ATGAGACCAT TTGGCAAGCA TGCGTCAGCC ACAGTCTTTA GTGACACTGT TGTGGTCAGC 
GAGAAATTCA TCAATGACTG TACAAAACTG TTCAGCGAAC GAATGCACCA AAAAGCTGAA 
AAGGAAATGA AAAATAATCC TGTGCATTTA ATCACAGAAG AAGATCTGAA ACAAATATCC 
ATTTTAGAAA GTGTTAATAC AAATAAAAAG GATAAAAAAG ATGAACGCAG GAAGAAAGCA 
ACAGAGGGTT CTGGAAGTGT AAGAGGCGGA GGAGGGGGCA ATGCCAGAGA ATACAAGATT 
AAGAAAGTCA AGAAGAAGGG AAGAAAAGAC GAGGACAGTG ATGATGAGTC TCAGTCATCG 
CATGCTGGAA AGAAGAAGCC AGACATCACC TTCATGTTCC AGGATGAAAT TGAAGGTTGC 
TTAAGGAAAC ACATTCCAGA TGCCCCTGAG GAGTTTATTT CAGAACTTGC AGAACACTTA 
ATAAAGCCTC TGAATAAAAT GTATCTTGAG GTGGTACGTT CAGTCTTCAT GTCGTCAACA 
TCTGCTTCTG GAACTGGCAG AAAACGCACG ATCAAGGACT TACAAGAAGA GGTTTCAAAT 
CTATACAATA ATATCAGATT ATTTGAAAAG GGGATGAAAT ATTTTGCAGA TGACACACAG 
ACTGCTCTGA CCAAGCACTT GCTGAAGACA GTGTGTACCG ATATCACTAA CCTCATGTTC 
AACTTCCTAG CCTCAGACTT GATGATGGCG GTGGAGGACC CTGCAACCAT TACAAGTGAT 
ATGAGAAAAA AAATTTTAAG CAAATTGACA GAAGAAACTA AAGTAGCTCT CACAAAACTC 
CACAACTCCC TGAATGAAAA AAGCATAGAA GACTTCCTTT CTTGTCTGGA TTCTGCAACA 
GAAGCTTGTG ACATTATGGT GAAAAAGGGA GACAAAAAAA GGGAAAGGCA AATCCTTTTC 
CAGCATCGGC AGGCTCTGGC TGACCAGCTG AAGGTCACAG AAGACCCTGC TCTGATTTTA 
CATCTCACAT CAGTCCTGTT ATTTCAGTTC TCAACCCACA GCATGCTCCA TGCACCTGGA 
AGATGTGTCC CACAGATCAT CGCCTTTCTT CACAACAAAA TCCCAGAGGA TCAACATACT 
CTCTTGGTAA AATATCAAGG ATTGGTTGTG AAACAGTTAG TCAGTCAAAA TAAGAAGAGT 
GGACAGGGAG AGGATCCGTC AAGCGATGAC TTAGACAAGG AACAACATGA CGTCACCAAT 
ACTACTCGGA AGGAGCTTCA GGAACTGTCT TTATCCATTA AGGACCTTGT TCTGAAGCCC 
AGGAAATCAT CTGTGACAGA GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006238021.1
CDS	643..2829
Translation	MRDELHVRGGRVNIVDLQQVINVDLTHIENRVGDIIKSEKHVQMVLGQLVDENYLDRLSEEVNDKLQESGQVTVSELCKTYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTAVNSLVSKYGFQEQLLYSVLEELVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDAVNYIKKRYKNTPLLFLKATCVGQGLVDQVEASVEEAISSGTWVDVSPLLPSSLSVEDAAMLLQQVMRPFGKHASATVFSDTVVVSEKFINDCTKLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTNKKDKKDERRKKATEGSGSVRGGGGGNAREYKIKKVKKKGRKDEDSDDESQSSHAGKKKPDITFMFQDEIEGCLRKHIPDAPEEFISELAEHLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYFADDTQTALTKHLLKTVCTDITNLMFNFLASDLMMAVEDPATITSDMRKKILSKLTEETKVALTKLHNSLNEKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALADQLKVTEDPALILHLTSVLLFQFSTHSMLHAPGRCVPQIIAFLHNKIPEDQHTLLVKYQGLVVKQLVSQNKKSGQGEDPSSDDLDKEQHDVTNTTRKELQELSLSIKDLVLKPRKSSVTEE

Target ORF information:

RefSeq Version	XM_006237959.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Ufm1-specific ligase 1 (Ufl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006237959.3

ORF Insert Sequence:

ATGAGAGATG AGCTACATGT CCGAGGTGGT CGAGTAAACA TTGTCGACTT ACAACAGGTT 
ATTAACGTGG ACCTGACTCA TATTGAAAAT AGAGTCGGCG ACATTATTAA GTCGGAGAAG 
CATGTGCAGA TGGTACTGGG ACAGCTGGTC GATGAGAACT ATCTGGATCG GTTATCAGAA 
GAGGTGAATG ATAAATTGCA AGAAAGTGGT CAAGTAACCG TCTCAGAGCT GTGTAAAACC 
TATGACCTTC CTGGGGATTT TCTGACACAG GCATTAACTC AGCGACTTGG CCGGATTATC 
AATGGCCATC TTGACCTTGA TAACAGAGGA GTGATTTTCA CTGAGGCCTT TGTGGCTCGC 
CATAAAGCAC GCATCCGTGG ACTCTTCAGT GCTATTACCC GGCCTACAGC AGTGAACTCT 
TTGGTTTCAA AATATGGATT TCAGGAACAG CTCCTTTACT CTGTGCTTGA GGAACTTGTT 
AGCACTGGCC GCCTACGAGG CACGGTGGTT GGCGGGCGAC AGGACAAAGC AGTGTTTGTC 
CCTGACATCT ATTCCAGGAC ACAGAGTACT TGGGTGGATT CCTTCTTCAG GCAGAACGGC 
TATCTAGAAT TTGACGCTTT ATCCAGACTT GGAATCCCAG ATGCTGTGAA CTATATAAAG 
AAAAGGTATA AGAATACCCC ACTCTTGTTT TTGAAGGCAA CTTGTGTTGG CCAAGGGCTC 
GTGGACCAGG TGGAAGCATC GGTAGAGGAG GCCATCAGCT CAGGAACATG GGTTGACGTT 
TCGCCTCTGC TGCCCAGTTC TTTGTCAGTT GAAGATGCTG CTATGCTGCT TCAGCAGGTG 
ATGAGACCAT TTGGCAAGCA TGCGTCAGCC ACAGTCTTTA GTGACACTGT TGTGGTCAGC 
GAGAAATTCA TCAATGACTG TACAAAACTG TTCAGCGAAC GAATGCACCA AAAAGCTGAA 
AAGGAAATGA AAAATAATCC TGTGCATTTA ATCACAGAAG AAGATCTGAA ACAAATATCC 
ATTTTAGAAA GTGTTAATAC AAATAAAAAG GATAAAAAAG ATGAACGCAG GAAGAAAGCA 
ACAGAGGGTT CTGGAAGTGT AAGAGGCGGA GGAGGGGGCA ATGCCAGAGA ATACAAGATT 
AAGAAAGTCA AGAAGAAGGG AAGAAAAGAC GAGGACAGTG ATGATGAGTC TCAGTCATCG 
CATGCTGGAA AGAAGAAGCC AGACATCACC TTCATGTTCC AGGATGAAAT TGAAGGTTGC 
TTAAGGAAAC ACATTCCAGA TGCCCCTGAG GAGTTTATTT CAGAACTTGC AGAACACTTA 
ATAAAGCCTC TGAATAAAAT GTATCTTGAG GTGGTACGTT CAGTCTTCAT GTCGTCAACA 
TCTGCTTCTG GAACTGGCAG AAAACGCACG ATCAAGGACT TACAAGAAGA GGTTTCAAAT 
CTATACAATA ATATCAGATT ATTTGAAAAG GGGATGAAAT ATTTTGCAGA TGACACACAG 
ACTGCTCTGA CCAAGCACTT GCTGAAGACA GTGTGTACCG ATATCACTAA CCTCATGTTC 
AACTTCCTAG CCTCAGACTT GATGATGGCG GTGGAGGACC CTGCAACCAT TACAAGTGAT 
ATGAGAAAAA AAATTTTAAG CAAATTGACA GAAGAAACTA AAGTAGCTCT CACAAAACTC 
CACAACTCCC TGAATGAAAA AAGCATAGAA GACTTCCTTT CTTGTCTGGA TTCTGCAACA 
GAAGCTTGTG ACATTATGGT GAAAAAGGGA GACAAAAAAA GGGAAAGGCA AATCCTTTTC 
CAGCATCGGC AGGCTCTGGC TGACCAGCTG AAGGTCACAG AAGACCCTGC TCTGATTTTA 
CATCTCACAT CAGTCCTGTT ATTTCAGTTC TCAACCCACA GCATGCTCCA TGCACCTGGA 
AGATGTGTCC CACAGATCAT CGCCTTTCTT CACAACAAAA TCCCAGAGGA TCAACATACT 
CTCTTGGTAA AATATCAAGG ATTGGTTGTG AAACAGTTAG TCAGTCAAAA TAAGAAGAGT 
GGACAGGGAG AGGATCCGTC AAGCGATGAC TTAGACAAGG AACAACATGA CGTCACCAAT 
ACTACTCGGA AGGAGCTTCA GGAACTGTCT TTATCCATTA AGGACCTTGT TCTGAAGCCC 
AGGAAATCAT CTGTGACAGA GGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modification of ASC1 by UFM1 is crucial for ER? transactivation and breast cancer development.
Molecular cell56(2)261-74(2014 Oct)
Yoo HM,Kang SH,Kim JY,Lee JE,Seong MW,Lee SW,Ka SH,Sou YS,Komatsu M,Tanaka K,Lee ST,Noh DY,Baek SH,Jeon YJ,Chung CH

Transcriptional regulation of the Ufm1 conjugation system in response to disturbance of the endoplasmic reticulum homeostasis and inhibition of vesicle trafficking.
PloS one7(11)e48587(2012)
Zhang Y,Zhang M,Wu J,Lei G,Li H

Ubiquitin fold modifier 1 (UFM1) and its target UFBP1 protect pancreatic beta cells from ER stress-induced apoptosis.
PloS one6(4)e18517(2011 Apr)
Lemaire K,Moura RF,Granvik M,Igoillo-Esteve M,Hohmeier HE,Hendrickx N,Newgard CB,Waelkens E,Cnop M,Schuit F

Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity.
The EMBO journal29(14)2446-60(2010 Jul)
Shiwaku H,Yoshimura N,Tamura T,Sone M,Ogishima S,Watase K,Tagawa K,Okazawa H

A novel C53/LZAP-interacting protein regulates stability of C53/LZAP and DDRGK domain-containing Protein 1 (DDRGK1) and modulates NF-kappaB signaling.
The Journal of biological chemistry285(20)15126-36(2010 May)
Wu J,Lei G,Mei M,Tang Y,Li H