ufl1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ufl1
Entrez Gene ID	100486377
Full Name	UFM1-specific ligase 1
Synonyms	maxer,nlbp,rcad
General protein information	Preferred Names UFM1-specific ligase 1 Names E3 UFM1-protein ligase 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934873.4	XP_002934919.2	E3 UFM1-protein ligase 1
XM_002934873.5	XP_002934919.3	E3 UFM1-protein ligase 1

Pathways from BioSystems

Homologies

	UFL1	XP_001100312.1
Canis lupus familiaris (dog)	UFL1	XP_532236.4
Bos taurus (cattle)	UFL1	NP_001073745.1
Anopheles gambiae (African malaria mosquito)	UFL1_ANOGA	XP_317778.3
Arabidopsis thaliana (thale cress)	AT3G46220	NP_566883.4
Homo sapiens (human)	UFL1	NP_056138.1
Gallus gallus (chicken)	UFL1	NP_001096671.1
Mus musculus (house mouse)	Ufl1	NP_080470.2
Rattus norvegicus (Norway rat)	Ufl1	NP_001119751.1
Drosophila melanogaster (fruit fly)	CG1104	NP_001246957.1
Oryza sativa (rice)	Os05g0117600	NP_001174184.1
Xenopus tropicalis (tropical clawed frog)	ufl1	XP_002934919.2
Pan troglodytes (chimpanzee)	UFL1	XP_518643.2
Danio rerio (zebrafish)	ufl1	NP_956900.1

Gene Ontology

The following ufl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ufl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa10446	XM_002934873.4 Latest version!	Xenopus tropicalis UFM1-specific ligase 1 (ufl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OXa58306	XM_002934873.5 Latest version!	Xenopus tropicalis UFM1-specific ligase 1 (ufl1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ufl1 ( XM_002934873.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934919.2 Xenopus tropicalis UFM1-specific ligase 1 (ufl1), mRNA.

Price & Availability↑

CloneID	OXa10446
Clone ID Related Accession (Same CDS sequence)	XM_002934873.4
Accession Version	XM_002934873.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 UFM1-protein ligase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934873.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCTG ATTGGGAAGA GATTCGGCGA CTGGCCGCTG ATTTTCAGCG GGCCCAGCTG 
AGTGATACAG TGCAGAGGTT ATCGGAACGT AACTGCATAG AAATTGTTGC CAAGTTAATT 
GCTGAAAAAC AACTGGATGT AGTACACACT CTTGATGGAA AGGAATATGT TACACCCTCG 
CAGATTACTA AAGAAATTAA GGATGAGCTA CAGATCCGAG GAGGTCGCGT CAACATAGTT 
GAACTTCAGC AGGTGATCAA TGTTGATTTG ACACATATCG AAAGCAGAGC CAATGATCTT 
GTTAAATTAG ATAAAGATAT CCAGCTTGTA CTTGGACAGC TTGTGGATGG CAACTATCTG 
GACCAAATTG CAGAAGAGGT TAACGATAAA CTGCAGGCAG CTGGCCAAGT GACTATATCA 
GAATTGTGCA AAACATACGA CCTTCCCGGA GACTTCCTGA AAGAGTCTTT CAGTTTGCGT 
CTTGGCAGGA TAATTCAGGG AAAGATCGAT GAAAGTAACA GAGGCGTATT CTATACAGAG 
GCTTTTGTGG CACAACATCG GGCACGTATA AGGGGCCTAT TTACAGCAAT CACAAAACCA 
ACACCTGTAA GCAATATGAT TTCCATTTAC GGCTTTCAGG AACATCTACT ATATTCTCTG 
TTGGAGGAGC TTGTTAACTG TGGACGTTTG AAAGGCACAG TGGTTGGTGG AAGACAGGAT 
AAGGCTGTGT TCATGCCTGA CATTTACGCC AAAACACAGA GCAACTGGAT AGACGCTTTT 
TTCAAGCAAA ACTGTTACCT AGAATTTGAT TCACTGTCCA GGCTTGGGAT TCCAGACCCT 
GTTGGATACA TCAAAAAGAG ATACAAGTCC ACACATATGT TGTATTTGAA GTCAGCATGC 
ATTGGCCAAG GCATAGTGGA TCACGTGGAA GCTTCTGTAG AGGAGGCAAT AAGCTCAGGG 
TCATGGTTGG ATATTATGCC TTTGCTTCCA AGTTGTCTTT CTCAAGAAGA TGCTGGAATT 
CTGCTTCAGC AAGTAATAAG AACACTGAAC AATTCTTCTT CTGCCCGAGT GTTTGGAGAT 
ACTATTGTTG TCAGTGAAAA ATTTATAAGT GAATGTGCTA ATCTATTTAA CAAACTTATG 
CACCACAAAG CTGAAAAGGA AATGAAGAGC AGCCCTGTGT TCATAGTGAC AGAAGAGGAT 
CTTAAGCAAG CAGCCATATT AGAAAATGAA GTTTTAAGCA AAAAGGATAA AAAGGAAGAA 
AGAAGGAAAA AAGCAACAGA GGGCAGTGGA AGCACTAGAA CTGGCGGTTG TGGTAATGCA 
AGAGAAATAA AAATAAAAAA GTCTAAGAAG AAAGCAAGAA AGGATGAAGA TGATGATGAA 
TCACAAACCT CATACCCAGG TAAACCAAAA GAAAGAGAAG CTGCATTTAT GTCTCTTGAA 
GAAATTCAAG ATGTTTTGAG AAAACATGCA CCGGACAGCC CAGATGAATT GGTCATTGAA 
CTCTCTGAAA ATCTAATTAG GCCCCTTACC AAGAGCTACC AGGAGGTAGT GCGGTCTGTG 
TTCTTGTCCT CAACCTCTTC CTCATCAGGA GCCAGTAGAA AACAGACCAT GAAAGACTTC 
CAAGAAGAAG TGACCAGTCT TTACAACAAC ATCCGGTTGT TTGAAAGGGG AATCAAATTC 
TTTACAGATG AAACTCAAAA CAACCTTGCC AAGCACCTGC TGAAGACAAT TTGCACAGAC 
ATTACAAATA TTATTTTTAA CTTCCTGGCA GCTGATTTTA TGATGGCAGC AGAAGATTAT 
ACCACCATCA CTAGTGAGGT TAGAAAAAAG ATGTTAAGCA AGCTCCAAGA GGAGGCTAAA 
GGCCCTTTGA CAAAGCTTCA TAGTTCATTA TCTGGAAAGA GTATTGAAGA ATTCTCATCC 
TATGTGGACT CAGCTGCAGA TGCATGTGGA ATATTAATGA AAAAAGGAGA TAAGAAGAAA 
GAAAGACAAA TTATGTTTCA GCATCGACAT GCTCTTATCG AACAGTTGAA AGTCACTGAA 
GATCCTGCTC TTGTGCTGCA TCTGACATCT GTTTTAATGT TTCAGTTGAC AACTCATTGT 
ATGCTCCATG CACCAGGGAG ATGTGTCCCA CAGATCATTG CTTTCCTTCA CACAAAGATT 
CCTGAGGACC AGCACAAACT TCTTGTTAAC TACCAGACTC TTGTTGTAAA GCAACTAATA 
GGCAATACCA AGCAGAAGGA TGAAGAAGAT GAGGACACAA TGGAGGACAC ACAAGAGGGG 
ACAGAAAAAG GAACTGATAA TATTAGAAAG GAACTTCAAG AAATGTCCCC TGTAATCAAG 
GATTTAGTTC TTCGATCTAG AAAATCATCC ATGAATGATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934919.2
CDS	96..2480
Translation	MSADWEEIRRLAADFQRAQLSDTVQRLSERNCIEIVAKLIAEKQLDVVHTLDGKEYVTPSQITKEIKDELQIRGGRVNIVELQQVINVDLTHIESRANDLVKLDKDIQLVLGQLVDGNYLDQIAEEVNDKLQAAGQVTISELCKTYDLPGDFLKESFSLRLGRIIQGKIDESNRGVFYTEAFVAQHRARIRGLFTAITKPTPVSNMISIYGFQEHLLYSLLEELVNCGRLKGTVVGGRQDKAVFMPDIYAKTQSNWIDAFFKQNCYLEFDSLSRLGIPDPVGYIKKRYKSTHMLYLKSACIGQGIVDHVEASVEEAISSGSWLDIMPLLPSCLSQEDAGILLQQVIRTLNNSSSARVFGDTIVVSEKFISECANLFNKLMHHKAEKEMKSSPVFIVTEEDLKQAAILENEVLSKKDKKEERRKKATEGSGSTRTGGCGNAREIKIKKSKKKARKDEDDDESQTSYPGKPKEREAAFMSLEEIQDVLRKHAPDSPDELVIELSENLIRPLTKSYQEVVRSVFLSSTSSSSGASRKQTMKDFQEEVTSLYNNIRLFERGIKFFTDETQNNLAKHLLKTICTDITNIIFNFLAADFMMAAEDYTTITSEVRKKMLSKLQEEAKGPLTKLHSSLSGKSIEEFSSYVDSAADACGILMKKGDKKKERQIMFQHRHALIEQLKVTEDPALVLHLTSVLMFQLTTHCMLHAPGRCVPQIIAFLHTKIPEDQHKLLVNYQTLVVKQLIGNTKQKDEEDEDTMEDTQEGTEKGTDNIRKELQEMSPVIKDLVLRSRKSSMNDE

Target ORF information:

RefSeq Version	XM_002934873.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis UFM1-specific ligase 1 (ufl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934873.4

ORF Insert Sequence:

ATGTCCGCTG ATTGGGAAGA GATTCGGCGA CTGGCCGCTG ATTTTCAGCG GGCCCAGCTG 
AGTGATACAG TGCAGAGGTT ATCGGAACGT AACTGCATAG AAATTGTTGC CAAGTTAATT 
GCTGAAAAAC AACTGGATGT AGTACACACT CTTGATGGAA AGGAATATGT TACACCCTCG 
CAGATTACTA AAGAAATTAA GGATGAGCTA CAGATCCGAG GAGGTCGCGT CAACATAGTT 
GAACTTCAGC AGGTGATCAA TGTTGATTTG ACACATATCG AAAGCAGAGC CAATGATCTT 
GTTAAATTAG ATAAAGATAT CCAGCTTGTA CTTGGACAGC TTGTGGATGG CAACTATCTG 
GACCAAATTG CAGAAGAGGT TAACGATAAA CTGCAGGCAG CTGGCCAAGT GACTATATCA 
GAATTGTGCA AAACATACGA CCTTCCCGGA GACTTCCTGA AAGAGTCTTT CAGTTTGCGT 
CTTGGCAGGA TAATTCAGGG AAAGATCGAT GAAAGTAACA GAGGCGTATT CTATACAGAG 
GCTTTTGTGG CACAACATCG GGCACGTATA AGGGGCCTAT TTACAGCAAT CACAAAACCA 
ACACCTGTAA GCAATATGAT TTCCATTTAC GGCTTTCAGG AACATCTACT ATATTCTCTG 
TTGGAGGAGC TTGTTAACTG TGGACGTTTG AAAGGCACAG TGGTTGGTGG AAGACAGGAT 
AAGGCTGTGT TCATGCCTGA CATTTACGCC AAAACACAGA GCAACTGGAT AGACGCTTTT 
TTCAAGCAAA ACTGTTACCT AGAATTTGAT TCACTGTCCA GGCTTGGGAT TCCAGACCCT 
GTTGGATACA TCAAAAAGAG ATACAAGTCC ACACATATGT TGTATTTGAA GTCAGCATGC 
ATTGGCCAAG GCATAGTGGA TCACGTGGAA GCTTCTGTAG AGGAGGCAAT AAGCTCAGGG 
TCATGGTTGG ATATTATGCC TTTGCTTCCA AGTTGTCTTT CTCAAGAAGA TGCTGGAATT 
CTGCTTCAGC AAGTAATAAG AACACTGAAC AATTCTTCTT CTGCCCGAGT GTTTGGAGAT 
ACTATTGTTG TCAGTGAAAA ATTTATAAGT GAATGTGCTA ATCTATTTAA CAAACTTATG 
CACCACAAAG CTGAAAAGGA AATGAAGAGC AGCCCTGTGT TCATAGTGAC AGAAGAGGAT 
CTTAAGCAAG CAGCCATATT AGAAAATGAA GTTTTAAGCA AAAAGGATAA AAAGGAAGAA 
AGAAGGAAAA AAGCAACAGA GGGCAGTGGA AGCACTAGAA CTGGCGGTTG TGGTAATGCA 
AGAGAAATAA AAATAAAAAA GTCTAAGAAG AAAGCAAGAA AGGATGAAGA TGATGATGAA 
TCACAAACCT CATACCCAGG TAAACCAAAA GAAAGAGAAG CTGCATTTAT GTCTCTTGAA 
GAAATTCAAG ATGTTTTGAG AAAACATGCA CCGGACAGCC CAGATGAATT GGTCATTGAA 
CTCTCTGAAA ATCTAATTAG GCCCCTTACC AAGAGCTACC AGGAGGTAGT GCGGTCTGTG 
TTCTTGTCCT CAACCTCTTC CTCATCAGGA GCCAGTAGAA AACAGACCAT GAAAGACTTC 
CAAGAAGAAG TGACCAGTCT TTACAACAAC ATCCGGTTGT TTGAAAGGGG AATCAAATTC 
TTTACAGATG AAACTCAAAA CAACCTTGCC AAGCACCTGC TGAAGACAAT TTGCACAGAC 
ATTACAAATA TTATTTTTAA CTTCCTGGCA GCTGATTTTA TGATGGCAGC AGAAGATTAT 
ACCACCATCA CTAGTGAGGT TAGAAAAAAG ATGTTAAGCA AGCTCCAAGA GGAGGCTAAA 
GGCCCTTTGA CAAAGCTTCA TAGTTCATTA TCTGGAAAGA GTATTGAAGA ATTCTCATCC 
TATGTGGACT CAGCTGCAGA TGCATGTGGA ATATTAATGA AAAAAGGAGA TAAGAAGAAA 
GAAAGACAAA TTATGTTTCA GCATCGACAT GCTCTTATCG AACAGTTGAA AGTCACTGAA 
GATCCTGCTC TTGTGCTGCA TCTGACATCT GTTTTAATGT TTCAGTTGAC AACTCATTGT 
ATGCTCCATG CACCAGGGAG ATGTGTCCCA CAGATCATTG CTTTCCTTCA CACAAAGATT 
CCTGAGGACC AGCACAAACT TCTTGTTAAC TACCAGACTC TTGTTGTAAA GCAACTAATA 
GGCAATACCA AGCAGAAGGA TGAAGAAGAT GAGGACACAA TGGAGGACAC ACAAGAGGGG 
ACAGAAAAAG GAACTGATAA TATTAGAAAG GAACTTCAAG AAATGTCCCC TGTAATCAAG 
GATTTAGTTC TTCGATCTAG AAAATCATCC ATGAATGATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ufl1 ( XM_002934873.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934919.3 Xenopus tropicalis UFM1-specific ligase 1 (ufl1), mRNA.

Price & Availability↑

CloneID	OXa58306
Clone ID Related Accession (Same CDS sequence)	XM_002934873.5
Accession Version	XM_002934873.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2385bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 UFM1-protein ligase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934873.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCTG ATTGGGAAGA GATTCGGCGA CTGGCCGCTG ATTTTCAGCG GGCCCAGCTG 
AGTGATACAG TGCAGAGGTT ATCGGAACGT AACTGCATAG AAATTGTTGC CAAGTTAATT 
GCTGAAAAAC AACTGGATGT AGTACACACT CTTGATGGAA AGGAATATGT TACACCCTCG 
CAGATTACTA AAGAAATTAA GGATGAGCTA CAGATCCGAG GAGGTCGCGT CAACATAGTT 
GAACTTCAGC AGGTGATCAA TGTTGATTTG ACACATATCG AAAGCAGAGC CAATGATCTT 
GTTAAATTAG ATAAAGATAT CCAGCTTGTA CTTGGACAGC TTGTGGATGG CAACTATCTG 
GACCAAATTG CAGAAGAGGT TAACGATAAA CTGCAGGCAG CTGGCCAAGT GACTATATCA 
GAATTGTGCA AAACATACGA CCTTCCCGGA GACTTCCTGA AAGAGTCTTT CAGTTTGCGT 
CTTGGCAGGA TAATTCAGGG AAAGATCGAT GAAAGTAACA GAGGCGTATT CTATACAGAG 
GCTTTTGTGG CACAACATCG GGCACGTATA AGGGGCCTAT TTACAGCAAT CACAAAACCA 
ACACCTGTAA GCAATATGAT TTCCATTTAC GGCTTTCAGG AACATCTACT ATATTCTCTG 
TTGGAGGAGC TTGTTAACTG TGGACGTTTG AAAGGCACAG TGGTTGGTGG AAGACAGGAT 
AAGGCTGTGT TCATGCCTGA CATTTACGCC AAAACACAGA GCAACTGGAT AGACGCTTTT 
TTCAAGCAAA ACTGTTACCT AGAATTTGAT TCACTGTCCA GGCTTGGGAT TCCAGACCCT 
GTTGGATACA TCAAAAAGAG ATACAAGTCC ACACATATGT TGTATTTGAA GTCAGCATGC 
ATTGGCCAAG GCATAGTGGA TCACGTGGAA GCTTCTGTAG AGGAGGCAAT AAGCTCAGGG 
TCATGGTTGG ATATTATGCC TTTGCTTCCA AGTTGTCTTT CTCAAGAAGA TGCTGGAATT 
CTGCTTCAGC AAGTAATAAG AACACTGAAC AATTCTTCTT CTGCCCGAGT GTTTGGAGAT 
ACTATTGTTG TCAGTGAAAA ATTTATAAGT GAATGTGCTA ATCTATTTAA CAAACTTATG 
CACCACAAAG CTGAAAAGGA AATGAAGAGC AGCCCTGTGT TCATAGTGAC AGAAGAGGAT 
CTTAAGCAAG CAGCCATATT AGAAAATGAA GTTTTAAGCA AAAAGGATAA AAAGGAAGAA 
AGAAGGAAAA AAGCAACAGA GGGCAGTGGA AGCACTAGAA CTGGCGGTTG TGGTAATGCA 
AGAGAAATAA AAATAAAAAA GTCTAAGAAG AAAGCAAGAA AGGATGAAGA TGATGATGAA 
TCACAAACCT CATACCCAGG TAAACCAAAA GAACGAGAAG CTGCATTTAT GTCTCTTGAA 
GAAATTCAAG ATGTTTTGAG AAAACATGCA CCGGACAGCC CAGATGAATT GGTCATTGAA 
CTCTCTGAAA ATCTAATTAG GCCCCTTACC AAGAGCTACC AGGAGGTAGT GCGGTCTGTG 
TTCTTGTCCT CAACCTCTTC CTCATCAGGA GCCAGTAGAA AACAGACCAT GAAAGACTTC 
CAAGAAGAAG TGACCAGTCT TTACAACAAC ATCCGGTTGT TTGAAAGGGG AATCAAATTC 
TTTACAGATG AAACTCAAAA CAACCTTGCC AAGCACCTGC TGAAGACAAT TTGCACAGAC 
ATTACAAATA TTATTTTTAA CTTCCTGGCA GCTGATTTTA TGATGGCAGC AGAAGATTAT 
ACCACCATCA CTAGTGAGGT TAGAAAAAAG ATGTTAAGCA AGCTCCAAGA GGAGGCTAAA 
GGCCCTTTGA CAAAGCTTCA TAGTTCATTA TCTGGAAAGA GTATTGAAGA ATTCTCATCC 
TATGTGGACT CAGCTGCAGA TGCATGTGGA ATATTAATGA AAAAAGGAGA TAAGAAGAAA 
GAAAGACAAA TTATGTTTCA GCATCGACAT GCTCTTATCG AACAGTTGAA AGTCACTGAA 
GATCCTGCTC TTGTGCTGCA TCTGACATCT GTTTTAATGT TTCAGTTGAC AACTCATTGT 
ATGCTCCATG CACCAGGGAG ATGTGTCCCA CAGATCATTG CTTTCCTTCA CACAAAGATT 
CCTGAGGACC AGCACAAACT TCTTGTTAAC TACCAGACTC TTGTTGTAAA GCAACTAATA 
GGCAATACCA AGCAGAAGGA TGAAGAAGAT GTGGACACAA TGGAGGACAC ACAAGAGGGG 
ACAGAAAAAG GAACTGATAA TATTAGAAAG GAACTTCAAG AAATGTCCCC TGTAATCAAG 
GATTTAGTTC TTCGATCTAG AAAATCATCC ATGAATGATG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934919.3
CDS	78..2462
Translation	MSADWEEIRRLAADFQRAQLSDTVQRLSERNCIEIVAKLIAEKQLDVVHTLDGKEYVTPSQITKEIKDELQIRGGRVNIVELQQVINVDLTHIESRANDLVKLDKDIQLVLGQLVDGNYLDQIAEEVNDKLQAAGQVTISELCKTYDLPGDFLKESFSLRLGRIIQGKIDESNRGVFYTEAFVAQHRARIRGLFTAITKPTPVSNMISIYGFQEHLLYSLLEELVNCGRLKGTVVGGRQDKAVFMPDIYAKTQSNWIDAFFKQNCYLEFDSLSRLGIPDPVGYIKKRYKSTHMLYLKSACIGQGIVDHVEASVEEAISSGSWLDIMPLLPSCLSQEDAGILLQQVIRTLNNSSSARVFGDTIVVSEKFISECANLFNKLMHHKAEKEMKSSPVFIVTEEDLKQAAILENEVLSKKDKKEERRKKATEGSGSTRTGGCGNAREIKIKKSKKKARKDEDDDESQTSYPGKPKEREAAFMSLEEIQDVLRKHAPDSPDELVIELSENLIRPLTKSYQEVVRSVFLSSTSSSSGASRKQTMKDFQEEVTSLYNNIRLFERGIKFFTDETQNNLAKHLLKTICTDITNIIFNFLAADFMMAAEDYTTITSEVRKKMLSKLQEEAKGPLTKLHSSLSGKSIEEFSSYVDSAADACGILMKKGDKKKERQIMFQHRHALIEQLKVTEDPALVLHLTSVLMFQLTTHCMLHAPGRCVPQIIAFLHTKIPEDQHKLLVNYQTLVVKQLIGNTKQKDEEDVDTMEDTQEGTEKGTDNIRKELQEMSPVIKDLVLRSRKSSMNDE

Target ORF information:

RefSeq Version	XM_002934873.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis UFM1-specific ligase 1 (ufl1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934873.5

ORF Insert Sequence:

ATGTCCGCTG ATTGGGAAGA GATTCGGCGA CTGGCCGCTG ATTTTCAGCG GGCCCAGCTG 
AGTGATACAG TGCAGAGGTT ATCGGAACGT AACTGCATAG AAATTGTTGC CAAGTTAATT 
GCTGAAAAAC AACTGGATGT AGTACACACT CTTGATGGAA AGGAATATGT TACACCCTCG 
CAGATTACTA AAGAAATTAA GGATGAGCTA CAGATCCGAG GAGGTCGCGT CAACATAGTT 
GAACTTCAGC AGGTGATCAA TGTTGATTTG ACACATATCG AAAGCAGAGC CAATGATCTT 
GTTAAATTAG ATAAAGATAT CCAGCTTGTA CTTGGACAGC TTGTGGATGG CAACTATCTG 
GACCAAATTG CAGAAGAGGT TAACGATAAA CTGCAGGCAG CTGGCCAAGT GACTATATCA 
GAATTGTGCA AAACATACGA CCTTCCCGGA GACTTCCTGA AAGAGTCTTT CAGTTTGCGT 
CTTGGCAGGA TAATTCAGGG AAAGATCGAT GAAAGTAACA GAGGCGTATT CTATACAGAG 
GCTTTTGTGG CACAACATCG GGCACGTATA AGGGGCCTAT TTACAGCAAT CACAAAACCA 
ACACCTGTAA GCAATATGAT TTCCATTTAC GGCTTTCAGG AACATCTACT ATATTCTCTG 
TTGGAGGAGC TTGTTAACTG TGGACGTTTG AAAGGCACAG TGGTTGGTGG AAGACAGGAT 
AAGGCTGTGT TCATGCCTGA CATTTACGCC AAAACACAGA GCAACTGGAT AGACGCTTTT 
TTCAAGCAAA ACTGTTACCT AGAATTTGAT TCACTGTCCA GGCTTGGGAT TCCAGACCCT 
GTTGGATACA TCAAAAAGAG ATACAAGTCC ACACATATGT TGTATTTGAA GTCAGCATGC 
ATTGGCCAAG GCATAGTGGA TCACGTGGAA GCTTCTGTAG AGGAGGCAAT AAGCTCAGGG 
TCATGGTTGG ATATTATGCC TTTGCTTCCA AGTTGTCTTT CTCAAGAAGA TGCTGGAATT 
CTGCTTCAGC AAGTAATAAG AACACTGAAC AATTCTTCTT CTGCCCGAGT GTTTGGAGAT 
ACTATTGTTG TCAGTGAAAA ATTTATAAGT GAATGTGCTA ATCTATTTAA CAAACTTATG 
CACCACAAAG CTGAAAAGGA AATGAAGAGC AGCCCTGTGT TCATAGTGAC AGAAGAGGAT 
CTTAAGCAAG CAGCCATATT AGAAAATGAA GTTTTAAGCA AAAAGGATAA AAAGGAAGAA 
AGAAGGAAAA AAGCAACAGA GGGCAGTGGA AGCACTAGAA CTGGCGGTTG TGGTAATGCA 
AGAGAAATAA AAATAAAAAA GTCTAAGAAG AAAGCAAGAA AGGATGAAGA TGATGATGAA 
TCACAAACCT CATACCCAGG TAAACCAAAA GAACGAGAAG CTGCATTTAT GTCTCTTGAA 
GAAATTCAAG ATGTTTTGAG AAAACATGCA CCGGACAGCC CAGATGAATT GGTCATTGAA 
CTCTCTGAAA ATCTAATTAG GCCCCTTACC AAGAGCTACC AGGAGGTAGT GCGGTCTGTG 
TTCTTGTCCT CAACCTCTTC CTCATCAGGA GCCAGTAGAA AACAGACCAT GAAAGACTTC 
CAAGAAGAAG TGACCAGTCT TTACAACAAC ATCCGGTTGT TTGAAAGGGG AATCAAATTC 
TTTACAGATG AAACTCAAAA CAACCTTGCC AAGCACCTGC TGAAGACAAT TTGCACAGAC 
ATTACAAATA TTATTTTTAA CTTCCTGGCA GCTGATTTTA TGATGGCAGC AGAAGATTAT 
ACCACCATCA CTAGTGAGGT TAGAAAAAAG ATGTTAAGCA AGCTCCAAGA GGAGGCTAAA 
GGCCCTTTGA CAAAGCTTCA TAGTTCATTA TCTGGAAAGA GTATTGAAGA ATTCTCATCC 
TATGTGGACT CAGCTGCAGA TGCATGTGGA ATATTAATGA AAAAAGGAGA TAAGAAGAAA 
GAAAGACAAA TTATGTTTCA GCATCGACAT GCTCTTATCG AACAGTTGAA AGTCACTGAA 
GATCCTGCTC TTGTGCTGCA TCTGACATCT GTTTTAATGT TTCAGTTGAC AACTCATTGT 
ATGCTCCATG CACCAGGGAG ATGTGTCCCA CAGATCATTG CTTTCCTTCA CACAAAGATT 
CCTGAGGACC AGCACAAACT TCTTGTTAAC TACCAGACTC TTGTTGTAAA GCAACTAATA 
GGCAATACCA AGCAGAAGGA TGAAGAAGAT GTGGACACAA TGGAGGACAC ACAAGAGGGG 
ACAGAAAAAG GAACTGATAA TATTAGAAAG GAACTTCAAG AAATGTCCCC TGTAATCAAG 
GATTTAGTTC TTCGATCTAG AAAATCATCC ATGAATGATG AATGA