Usp33 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp33
Entrez Gene ID	310960
Full Name	ubiquitin specific peptidase 33
General protein information	Preferred Names ubiquitin specific peptidase 33 Names ubiquitin carboxyl-terminal hydrolase 33 ubiquitin specific protease 33
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q45

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191094.1	NP_001178023.1	ubiquitin carboxyl-terminal hydrolase 33
XM_006233492.2	XP_006233554.1	ubiquitin carboxyl-terminal hydrolase 33 isoform X1
XM_006233493.2	XP_006233555.1	ubiquitin carboxyl-terminal hydrolase 33 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	USP33	XP_003308213.1
	LOC710693	XP_002808266.1
Xenopus tropicalis (tropical clawed frog)	usp33	NP_001016228.1
Mus musculus (house mouse)	Usp33	NP_573510.2
Homo sapiens (human)	USP33	NP_055832.3
Rattus norvegicus (Norway rat)	Usp33	NP_001178023.1
Danio rerio (zebrafish)	usp33	NP_998392.1
Bos taurus (cattle)	USP33	NP_001094609.1
Canis lupus familiaris (dog)	USP33	XP_005622106.1
Gallus gallus (chicken)	USP33	XP_004936727.1

Gene Ontology
Bibliography

Related articles in PubMed

Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.
Zhang X, Jiang Y, Han W, Liu A, Xie X, Han C, Fan C, Wang H, Zhang H, Ding S, Shan Z, Teng W
Biological trace element research174(2)377-386(2016 Dec)

The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.
Simicek M, Lievens S, Laga M, Guzenko D, Aushev VN, Kalev P, Baietti MF, Strelkov SV, Gevaert K, Tavernier J, Sablina AA
Nature cell biology15(10)1220-30(2013 Oct)

USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110.
Li J, D'Angiolella V, Seeley ES, Kim S, Kobayashi T, Fu W, Campos EI, Pagano M, Dynlacht BD
Nature495(7440)255-9(2013 Mar)

Isoform-specific localization of the deubiquitinase USP33 to the Golgi apparatus.
Thorne C, Eccles RL, Coulson JM, Urb? S, Clague MJ
Traffic (Copenhagen, Denmark)12(11)1563-74(2011 Nov)

Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration.
Yuasa-Kawada J, Kinoshita-Kawada M, Rao Y, Wu JY
Proceedings of the National Academy of Sciences of the United States of America106(34)14530-5(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High iodine intake had no discernible effects on USP20. Temporary increases in USP33 and USP19 mRNA levels were observed. The enzymes related to D2 ubiquitination change with prolonged high iodine intake. Increased D2 ubiquitination suppresses the activity of D2, causing a decrease in negative feedback of the hypothalamic-pituitary-thyroid axis.
Title: Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.

Correlation between relative mRNA levels of WSB-1 and USP-33 in numerous tissues that do not express type 2 deiodinase(D2) suggests that these ubiquitin-related enzymes share additional substrates besides D2.
Title: Expression patterns of WSB-1 and USP-33 underlie cell-specific posttranslational control of type 2 deiodinase in the rat brain.

The following Usp33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa06876	NM_001191094.1 Latest version!	Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa25371	XM_006233492.2 Latest version!	Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa25372	XM_006233493.2 Latest version!	Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp33 ( NM_001191094.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178023.1 Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), mRNA.

Price & Availability↑

CloneID	ORa06876
Clone ID Related Accession (Same CDS sequence)	NM_001191094.1
Accession Version	NM_001191094.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 33
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07013807.1. On Jul 17, 2010 this sequence version replaced XM_001068666.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACAAAAGAG 
GATTTGATAC AAAAATCTCA TGGTGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCATGCC TAGAGAATAG GTGTTCCTAT GTTGGCTGCG GGGAGTCACA AGATCATAGC 
ACCATACACT CGAAGAAGAC AAAGCATTAT CTAACTGTGA ACCTCACCAC CCTTCGAGTA 
TGGTGTTATG CTTGCAGCAA AGAAGTATTC TTGGATAGAA AATTAGGAAC TCCTCCTTCA 
TTACCTCATG TAAGACAGCC TCAACAAACA CAAGAAAACA GTGTCCAGGA TTTTAAAATA 
CCCAGTAATA CAGCATTGAA AACCCCTCTG GTTGCTGTAT TTGAAGATCT GGATATAGAA 
GTGGAAGAGG AAGATGAGCT GAAGGCTAGA GGCCTGACAG GTTTGAAAAA CATTGGAAAT 
ACTTGCTATA TGAATGCCGC TCTGCAAGCT CTCTCTAACT GCCCACCTTT GACACAGTTC 
TTTCTTGATT GTGGAGGACT GGCTAGAACA GATAAGAAAC CAGCCATTTG TAAAAGTTAT 
CTCAAACTAA TGACAGAATT GTGGCACAAG AGCAGGCCAG GATCTGTTGT GCCTGCTAAT 
CTGTTCCAAG GAATTAAAAC TGTAAATCCA ACTTTTCGAG GCTATTCTCA GCAGGATGCC 
CAGGAATTCC TTCGCTGTTT AATGGATCTG CTTCATGAAG AGTTGAAGGA GCAGGTCATG 
GAAATAGAGG AAGAGCCTCA AGCACTAACT TCTGAGGAGA CAGTGGAGGA AGAGAAGAGC 
CAGTCAGATG TGGATTTTCA GTCCTGCGAG TCATGTAGCA GCAGCAGTGA GAAAGGGGAA 
AATGAGAGTG TCTCTAAGGG CGGCCCTGAA GACAGCACTG AGACCACCAT GCTCATTCAG 
GATGAGGAGG ACCTAGAGAT GGCCAAAGAC TGGCAAAAAG AGAAGATGTG CAACAAGAGC 
AACAAAGCAG ATTCTGACGG AGAACCGGAT AAAGACAGAG ACACGGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAGGTG CAGATACACG GCAGAGCCTC AGAATATATC 
ACTGATGTCC ATTTGAATGA CCTATCTACA CCACAGATCC TTCCATCAAA TGAAAGTGTT 
AATCCACGTT TATCAGCAAG TCCTCCTAAG TCAGGCAATC TGTGGCCAGG ATTGACACCT 
CCACACAAGA AAGCTCAGTC TTCATCTCCA AAGAGGAAAA AGCAGCATAA GAAGTACAGA 
AGTGTCATCT CTGACATATT TGATGGGACA ATCATTAGTT CAGTACAGTG TCTGACGTGT 
GATAGGGTGT CTGTAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAAGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCGTGT 
GGCGAAGCCT ACGCTCCACA GGGGTGGATA GCTTTCTTCA TGGAGTACGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTAAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ATAATATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAAAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTGGAC 
CTTCAGCCAT TTCTTGCAAA GGATAGCCCA GCTCAGATCG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ATCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CAGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACC 
GTACAGAATG CCGAAGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAGG 
GAGAGGCGAA GGATATCCAA TTTGTTGAAC ATAATGGAGC CAAGCCTCCT TCAGTTCTAT 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGACTTTC TCTGTATTCA TGGAGGTATT CCTCCACGAA AAGCAAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ACAGCAGGTA TGGAGGAGGG 
CCTGCTGTCA ACCACCTCTA CATTTGTCAC ACTTGCCAAA TTGAGGCAGA GAAGATTGAA 
AAACGAAGAA AAACTGAATT GGAAATTTTT ATTCGGCTCA ACAGAGCATT TCAAGAGGAA 
GACTCTCCAG CTACTTTTTA CTGTATCAGC ATGCAGTGGT TTAGAGAATG GGAGAGTTTT 
GTAAAGGATC CTCCAGGTCC AATAGACAAC ACTAAAATTG CAGTCACTAA GTGTGGTAAT 
GTGATGCTCA AGCAAGGAGC AGATTCTGGT CAAATTTCAG AAGAAACATG GAATTTCCTG 
CAATCTATAT ATGGTGGGGG ACCTGAAGTT ATCCTCCGAC CTCCAGTTGT TCATGTTGAC 
CCTGATGCAC TACAAGCAGA GGAAAAGATT GAAGTAGAAA CTCGCTCTTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178023.1
CDS	1..2694
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSHGACQDCKVRGPNLWACLENRCSYVGCGESQDHSTIHSKKTKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNTALKTPLVAVFEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEIEEEPQALTSEETVEEEKSQSDVDFQSCESCSSSSEKGENESVSKGGPEDSTETTMLIQDEEDLEMAKDWQKEKMCNKSNKADSDGEPDKDRDTVCETVDLNSQETVKVQIHGRASEYITDVHLNDLSTPQILPSNESVNPRLSASPPKSGNLWPGLTPPHKKAQSSSPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQRERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRLNRAFQEEDSPATFYCISMQWFREWESFVKDPPGPIDNTKIAVTKCGNVMLKQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDALQAEEKIEVETRSL

Target ORF information:

RefSeq Version	NM_001191094.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191094.1

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACAAAAGAG 
GATTTGATAC AAAAATCTCA TGGTGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCATGCC TAGAGAATAG GTGTTCCTAT GTTGGCTGCG GGGAGTCACA AGATCATAGC 
ACCATACACT CGAAGAAGAC AAAGCATTAT CTAACTGTGA ACCTCACCAC CCTTCGAGTA 
TGGTGTTATG CTTGCAGCAA AGAAGTATTC TTGGATAGAA AATTAGGAAC TCCTCCTTCA 
TTACCTCATG TAAGACAGCC TCAACAAACA CAAGAAAACA GTGTCCAGGA TTTTAAAATA 
CCCAGTAATA CAGCATTGAA AACCCCTCTG GTTGCTGTAT TTGAAGATCT GGATATAGAA 
GTGGAAGAGG AAGATGAGCT GAAGGCTAGA GGCCTGACAG GTTTGAAAAA CATTGGAAAT 
ACTTGCTATA TGAATGCCGC TCTGCAAGCT CTCTCTAACT GCCCACCTTT GACACAGTTC 
TTTCTTGATT GTGGAGGACT GGCTAGAACA GATAAGAAAC CAGCCATTTG TAAAAGTTAT 
CTCAAACTAA TGACAGAATT GTGGCACAAG AGCAGGCCAG GATCTGTTGT GCCTGCTAAT 
CTGTTCCAAG GAATTAAAAC TGTAAATCCA ACTTTTCGAG GCTATTCTCA GCAGGATGCC 
CAGGAATTCC TTCGCTGTTT AATGGATCTG CTTCATGAAG AGTTGAAGGA GCAGGTCATG 
GAAATAGAGG AAGAGCCTCA AGCACTAACT TCTGAGGAGA CAGTGGAGGA AGAGAAGAGC 
CAGTCAGATG TGGATTTTCA GTCCTGCGAG TCATGTAGCA GCAGCAGTGA GAAAGGGGAA 
AATGAGAGTG TCTCTAAGGG CGGCCCTGAA GACAGCACTG AGACCACCAT GCTCATTCAG 
GATGAGGAGG ACCTAGAGAT GGCCAAAGAC TGGCAAAAAG AGAAGATGTG CAACAAGAGC 
AACAAAGCAG ATTCTGACGG AGAACCGGAT AAAGACAGAG ACACGGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAGGTG CAGATACACG GCAGAGCCTC AGAATATATC 
ACTGATGTCC ATTTGAATGA CCTATCTACA CCACAGATCC TTCCATCAAA TGAAAGTGTT 
AATCCACGTT TATCAGCAAG TCCTCCTAAG TCAGGCAATC TGTGGCCAGG ATTGACACCT 
CCACACAAGA AAGCTCAGTC TTCATCTCCA AAGAGGAAAA AGCAGCATAA GAAGTACAGA 
AGTGTCATCT CTGACATATT TGATGGGACA ATCATTAGTT CAGTACAGTG TCTGACGTGT 
GATAGGGTGT CTGTAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAAGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCGTGT 
GGCGAAGCCT ACGCTCCACA GGGGTGGATA GCTTTCTTCA TGGAGTACGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTAAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ATAATATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAAAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTGGAC 
CTTCAGCCAT TTCTTGCAAA GGATAGCCCA GCTCAGATCG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ATCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CAGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACC 
GTACAGAATG CCGAAGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAGG 
GAGAGGCGAA GGATATCCAA TTTGTTGAAC ATAATGGAGC CAAGCCTCCT TCAGTTCTAT 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGACTTTC TCTGTATTCA TGGAGGTATT CCTCCACGAA AAGCAAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ACAGCAGGTA TGGAGGAGGG 
CCTGCTGTCA ACCACCTCTA CATTTGTCAC ACTTGCCAAA TTGAGGCAGA GAAGATTGAA 
AAACGAAGAA AAACTGAATT GGAAATTTTT ATTCGGCTCA ACAGAGCATT TCAAGAGGAA 
GACTCTCCAG CTACTTTTTA CTGTATCAGC ATGCAGTGGT TTAGAGAATG GGAGAGTTTT 
GTAAAGGATC CTCCAGGTCC AATAGACAAC ACTAAAATTG CAGTCACTAA GTGTGGTAAT 
GTGATGCTCA AGCAAGGAGC AGATTCTGGT CAAATTTCAG AAGAAACATG GAATTTCCTG 
CAATCTATAT ATGGTGGGGG ACCTGAAGTT ATCCTCCGAC CTCCAGTTGT TCATGTTGAC 
CCTGATGCAC TACAAGCAGA GGAAAAGATT GAAGTAGAAA CTCGCTCTTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp33 ( XM_006233492.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233554.1 Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25371
Clone ID Related Accession (Same CDS sequence)	XM_006233492.2
Accession Version	XM_006233492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACAAAAGAG 
GATTTGATAC AAAAATCTCA TGGTGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCATGCC TAGAGAATAG GTGTTCCTAT GTTGGCTGCG GGGAGTCACA AGATCATAGC 
ACCATACACT CGAAGAAGAC AAAGCATTAT CTAACTGTGA ACCTCACCAC CCTTCGAGTA 
TGGTGTTATG CTTGCAGCAA AGAAGTATTC TTGGATAGAA AATTAGGAAC TCCTCCTTCA 
TTACCTCATG TAAGACAGCC TCAACAAACA CAAGAAAACA GTGTCCAGGA TTTTAAAATA 
CCCAGTAATA CAGCATTGAA AACCCCTCTG GTTGCTGTAT TTGAAGATCT GGATATAGAA 
GTGGAAGAGG AAGATGAGCT GAAGGCTAGA GGCCTGACAG GTTTGAAAAA CATTGGAAAT 
ACTTGCTATA TGAATGCCGC TCTGCAAGCT CTCTCTAACT GCCCACCTTT GACACAGTTC 
TTTCTTGATT GTGGAGGACT GGCTAGAACA GATAAGAAAC CAGCCATTTG TAAAAGTTAT 
CTCAAACTAA TGACAGAATT GTGGCACAAG AGCAGGCCAG GATCTGTTGT GCCTGCTAAT 
CTGTTCCAAG GAATTAAAAC TGTAAATCCA ACTTTTCGAG GCTATTCTCA GCAGGATGCC 
CAGGAATTCC TTCGCTGTTT AATGGATCTG CTTCATGAAG AGTTGAAGGA GCAGGTCATG 
GAAATAGAGG AAGAGCCTCA AGCACTAACT TCTGAGGAGA CAGTGGAGGA AGAGAAGAGC 
CAGTCAGATG TGGATTTTCA GTCCTGCGAG TCATGTAGCA GCAGCAGTGA GAAAGGGGAA 
AATGAGAGTG TCTCTAAGGG CGGCCCTGAA GACAGCACTG AGACCACCAT GCTCATTCAG 
GATGAGGAGG ACCTAGAGAT GGCCAAAGAC TGGCAAAAAG AGAAGATGTG CAACAAGAGC 
AACAAAGCAG ATTCTGACGG AGAACCGGAT AAAGACAGAG ACACGGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAGGTG CAGATACACG GCAGAGCCTC AGAATATATC 
ACTGATGTCC ATTTGAATGA CCTATCTACA CCACAGATCC TTCCATCAAA TGAAAGTGTT 
AATCCACGTT TATCAGCAAG TCCTCCTAAG TCAGGCAATC TGTGGCCAGG ATTGACACCT 
CCACACAAGA AAGCTCAGTC TTCATCTCCA AAGAGGAAAA AGCAGCATAA GAAGTACAGA 
AGTGTCATCT CTGACATATT TGATGGGACA ATCATTAGTT CAGTACAGTG TCTGACGTGT 
GATAGGGTGT CTGTAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAAGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCGTGT 
GGCGAAGCCT ACGCTCCACA GGGGTGGATA GCTTTCTTCA TGGAGTACGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TAACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGATAATA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAAA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT GGACCTTCAG CCATTTCTTG CAAAGGATAG CCCAGCTCAG 
ATCGTGACAT ATGATCTCCT GTCAGTCATC TGTCATCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCAGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACCGTACAG AATGCCGAAG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAGGGAGAGG CGAAGGATAT CCAATTTGTT GAACATAATG 
GAGCCAAGCC TCCTTCAGTT CTATATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAC TTTCTCTGTA TTCATGGAGG TATTCCTCCA 
CGAAAAGCAA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTACAGCA GGTATGGAGG AGGGCCTGCT GTCAACCACC TCTACATTTG TCACACTTGC 
CAAATTGAGG CAGAGAAGAT TGAAAAACGA AGAAAAACTG AATTGGAAAT TTTTATTCGG 
CTCAACAGAG CATTTCAAGA GGAAGACTCT CCAGCTACTT TTTACTGTAT CAGCATGCAG 
TGGTTTAGAG AATGGGAGAG TTTTGTAAAG GGTAAGGATG GAGATCCTCC AGGTCCAATA 
GACAACACTA AAATTGCAGT CACTAAGTGT GGTAATGTGA TGCTCAAGCA AGGAGCAGAT 
TCTGGTCAAA TTTCAGAAGA AACATGGAAT TTCCTGCAAT CTATATATGG TGGGGGACCT 
GAAGTTATCC TCCGACCTCC AGTTGTTCAT GTTGACCCTG ATGCACTACA AGCAGAGGAA 
AAGATTGAAG TAGAAACTCG CTCTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233554.1
CDS	166..2895
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSHGACQDCKVRGPNLWACLENRCSYVGCGESQDHSTIHSKKTKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNTALKTPLVAVFEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEIEEEPQALTSEETVEEEKSQSDVDFQSCESCSSSSEKGENESVSKGGPEDSTETTMLIQDEEDLEMAKDWQKEKMCNKSNKADSDGEPDKDRDTVCETVDLNSQETVKVQIHGRASEYITDVHLNDLSTPQILPSNESVNPRLSASPPKSGNLWPGLTPPHKKAQSSSPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKRFVVSCVPSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQRERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRLNRAFQEEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLKQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDALQAEEKIEVETRSL

Target ORF information:

RefSeq Version	XM_006233492.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233492.2

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACAAAAGAG 
GATTTGATAC AAAAATCTCA TGGTGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCATGCC TAGAGAATAG GTGTTCCTAT GTTGGCTGCG GGGAGTCACA AGATCATAGC 
ACCATACACT CGAAGAAGAC AAAGCATTAT CTAACTGTGA ACCTCACCAC CCTTCGAGTA 
TGGTGTTATG CTTGCAGCAA AGAAGTATTC TTGGATAGAA AATTAGGAAC TCCTCCTTCA 
TTACCTCATG TAAGACAGCC TCAACAAACA CAAGAAAACA GTGTCCAGGA TTTTAAAATA 
CCCAGTAATA CAGCATTGAA AACCCCTCTG GTTGCTGTAT TTGAAGATCT GGATATAGAA 
GTGGAAGAGG AAGATGAGCT GAAGGCTAGA GGCCTGACAG GTTTGAAAAA CATTGGAAAT 
ACTTGCTATA TGAATGCCGC TCTGCAAGCT CTCTCTAACT GCCCACCTTT GACACAGTTC 
TTTCTTGATT GTGGAGGACT GGCTAGAACA GATAAGAAAC CAGCCATTTG TAAAAGTTAT 
CTCAAACTAA TGACAGAATT GTGGCACAAG AGCAGGCCAG GATCTGTTGT GCCTGCTAAT 
CTGTTCCAAG GAATTAAAAC TGTAAATCCA ACTTTTCGAG GCTATTCTCA GCAGGATGCC 
CAGGAATTCC TTCGCTGTTT AATGGATCTG CTTCATGAAG AGTTGAAGGA GCAGGTCATG 
GAAATAGAGG AAGAGCCTCA AGCACTAACT TCTGAGGAGA CAGTGGAGGA AGAGAAGAGC 
CAGTCAGATG TGGATTTTCA GTCCTGCGAG TCATGTAGCA GCAGCAGTGA GAAAGGGGAA 
AATGAGAGTG TCTCTAAGGG CGGCCCTGAA GACAGCACTG AGACCACCAT GCTCATTCAG 
GATGAGGAGG ACCTAGAGAT GGCCAAAGAC TGGCAAAAAG AGAAGATGTG CAACAAGAGC 
AACAAAGCAG ATTCTGACGG AGAACCGGAT AAAGACAGAG ACACGGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAGGTG CAGATACACG GCAGAGCCTC AGAATATATC 
ACTGATGTCC ATTTGAATGA CCTATCTACA CCACAGATCC TTCCATCAAA TGAAAGTGTT 
AATCCACGTT TATCAGCAAG TCCTCCTAAG TCAGGCAATC TGTGGCCAGG ATTGACACCT 
CCACACAAGA AAGCTCAGTC TTCATCTCCA AAGAGGAAAA AGCAGCATAA GAAGTACAGA 
AGTGTCATCT CTGACATATT TGATGGGACA ATCATTAGTT CAGTACAGTG TCTGACGTGT 
GATAGGGTGT CTGTAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAAGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCGTGT 
GGCGAAGCCT ACGCTCCACA GGGGTGGATA GCTTTCTTCA TGGAGTACGT GAAGAGGTTT 
GTTGTCTCAT GTGTCCCTAG CTGGTTTTGG GGTCCAGTAG TAACCTTGCA AGATTGTCTT 
GCTGCCTTCT TCGCCAGAGA TGAACTTAAA GGTGATAATA TGTACAGTTG TGAAAAATGC 
AAAAAGTTGA GGAATGGAGT AAAGTTTTGT AAAGTACAAA AGTTTCCTGA GATTTTGTGT 
ATCCACCTTA AAAGATTTCG ACATGAACTG ATGTTTTCCA CCAAAATTAG CACCCATGTT 
TCCTTCCCCC TGGAAGGCCT GGACCTTCAG CCATTTCTTG CAAAGGATAG CCCAGCTCAG 
ATCGTGACAT ATGATCTCCT GTCAGTCATC TGTCATCATG GGACTGCAAG TAGTGGGCAC 
TACATTGCCT ACTGCAGAAA CAATTTAAAT AACCTGTGGT ATGAGTTTGA TGACCAGAGC 
GTCACTGAAG TTTCAGAGTC CACCGTACAG AATGCCGAAG CCTACGTCCT TTTCTACAGG 
AAGAGCAGTG AAGAGGCACA AAGGGAGAGG CGAAGGATAT CCAATTTGTT GAACATAATG 
GAGCCAAGCC TCCTTCAGTT CTATATATCT CGACAGTGGT TGAATAAATT TAAGACCTTT 
GCTGAACCTG GCCCTATTTC AAATAATGAC TTTCTCTGTA TTCATGGAGG TATTCCTCCA 
CGAAAAGCAA GTTATATTGA AGACTTAGTT TTGATGCTGC CTCAGAACAT TTGGGATAAC 
CTCTACAGCA GGTATGGAGG AGGGCCTGCT GTCAACCACC TCTACATTTG TCACACTTGC 
CAAATTGAGG CAGAGAAGAT TGAAAAACGA AGAAAAACTG AATTGGAAAT TTTTATTCGG 
CTCAACAGAG CATTTCAAGA GGAAGACTCT CCAGCTACTT TTTACTGTAT CAGCATGCAG 
TGGTTTAGAG AATGGGAGAG TTTTGTAAAG GGTAAGGATG GAGATCCTCC AGGTCCAATA 
GACAACACTA AAATTGCAGT CACTAAGTGT GGTAATGTGA TGCTCAAGCA AGGAGCAGAT 
TCTGGTCAAA TTTCAGAAGA AACATGGAAT TTCCTGCAAT CTATATATGG TGGGGGACCT 
GAAGTTATCC TCCGACCTCC AGTTGTTCAT GTTGACCCTG ATGCACTACA AGCAGAGGAA 
AAGATTGAAG TAGAAACTCG CTCTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp33 ( XM_006233493.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233555.1 Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), transcript variant X2, mRNA.

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CloneID	ORa25372
Clone ID Related Accession (Same CDS sequence)	XM_006233493.2
Accession Version	XM_006233493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 33 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006233493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACAAAAGAG 
GATTTGATAC AAAAATCTCA TGGTGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCATGCC TAGAGAATAG GTGTTCCTAT GTTGGCTGCG GGGAGTCACA AGATCATAGC 
ACCATACACT CGAAGAAGAC AAAGCATTAT CTAACTGTGA ACCTCACCAC CCTTCGAGTA 
TGGTGTTATG CTTGCAGCAA AGAAGTATTC TTGGATAGAA AATTAGGAAC TCCTCCTTCA 
TTACCTCATG TAAGACAGCC TCAACAAACA CAAGAAAACA GTGTCCAGGA TTTTAAAATA 
CCCAGTAATA CAGCATTGAA AACCCCTCTG GTTGCTGTAT TTGAAGATCT GGATATAGAA 
GTGGAAGAGG AAGATGAGCT GAAGGCTAGA GGCCTGACAG GTTTGAAAAA CATTGGAAAT 
ACTTGCTATA TGAATGCCGC TCTGCAAGCT CTCTCTAACT GCCCACCTTT GACACAGTTC 
TTTCTTGATT GTGGAGGACT GGCTAGAACA GATAAGAAAC CAGCCATTTG TAAAAGTTAT 
CTCAAACTAA TGACAGAATT GTGGCACAAG AGCAGGCCAG GATCTGTTGT GCCTGCTAAT 
CTGTTCCAAG GAATTAAAAC TGTAAATCCA ACTTTTCGAG GCTATTCTCA GCAGGATGCC 
CAGGAATTCC TTCGCTGTTT AATGGATCTG CTTCATGAAG AGTTGAAGGA GCAGGTCATG 
GAAATAGAGG AAGAGCCTCA AGCACTAACT TCTGAGGAGA CAGTGGAGGA AGAGAAGAGC 
CAGTCAGATG TGGATTTTCA GTCCTGCGAG TCATGTAGCA GCAGCAGTGA GAAAGGGGAA 
AATGAGAGTG TCTCTAAGGG CGGCCCTGAA GACAGCACTG AGACCACCAT GCTCATTCAG 
GATGAGGAGG ACCTAGAGAT GGCCAAAGAC TGGCAAAAAG AGAAGATGTG CAACAAGAGC 
AACAAAGCAG ATTCTGACGG AGAACCGGAT AAAGACAGAG ACACGGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAGGTG CAGATACACG GCAGAGCCTC AGAATATATC 
ACTGATGTCC ATTTGAATGA CCTATCTACA CCACAGATCC TTCCATCAAA TGAAAGTGTT 
AATCCACGTT TATCAGCAAG TCCTCCTAAG TCAGGCAATC TGTGGCCAGG ATTGACACCT 
CCACACAAGA AAGCTCAGTC TTCATCTCCA AAGAGGAAAA AGCAGCATAA GAAGTACAGA 
AGTGTCATCT CTGACATATT TGATGGGACA ATCATTAGTT CAGTACAGTG TCTGACGTGT 
GATAGGGTGT CTGTAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAAGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCGTGT 
GGCGAAGCCT ACGCTCCACA GGGGTGGATA GCTTTCTTCA TGGAGTACGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTAAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ATAATATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAAAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTGGAC 
CTTCAGCCAT TTCTTGCAAA GGATAGCCCA GCTCAGATCG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ATCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CAGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACC 
GTACAGAATG CCGAAGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAGG 
GAGAGGCGAA GGATATCCAA TTTGTTGAAC ATAATGGAGC CAAGCCTCCT TCAGTTCTAT 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGACTTTC TCTGTATTCA TGGAGGTATT CCTCCACGAA AAGCAAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ACAGCAGGTA TGGAGGAGGG 
CCTGCTGTCA ACCACCTCTA CATTTGTCAC ACTTGCCAAA TTGAGGCAGA GAAGATTGAA 
AAACGAAGAA AAACTGAATT GGAAATTTTT ATTCGGCTCA ACAGAGCATT TCAAGAGGAA 
GACTCTCCAG CTACTTTTTA CTGTATCAGC ATGCAGTGGT TTAGAGAATG GGAGAGTTTT 
GTAAAGGGTA AGGATGGAGA TCCTCCAGGT CCAATAGACA ACACTAAAAT TGCAGTCACT 
AAGTGTGGTA ATGTGATGCT CAAGCAAGGA GCAGATTCTG GTCAAATTTC AGAAGAAACA 
TGGAATTTCC TGCAATCTAT ATATGGTGGG GGACCTGAAG TTATCCTCCG ACCTCCAGTT 
GTTCATGTTG ACCCTGATGC ACTACAAGCA GAGGAAAAGA TTGAAGTAGA AACTCGCTCT 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233555.1
CDS	166..2871
Translation	MTTFRNHCPHLDSVGEITKEDLIQKSHGACQDCKVRGPNLWACLENRCSYVGCGESQDHSTIHSKKTKHYLTVNLTTLRVWCYACSKEVFLDRKLGTPPSLPHVRQPQQTQENSVQDFKIPSNTALKTPLVAVFEDLDIEVEEEDELKARGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLDCGGLARTDKKPAICKSYLKLMTELWHKSRPGSVVPANLFQGIKTVNPTFRGYSQQDAQEFLRCLMDLLHEELKEQVMEIEEEPQALTSEETVEEEKSQSDVDFQSCESCSSSSEKGENESVSKGGPEDSTETTMLIQDEEDLEMAKDWQKEKMCNKSNKADSDGEPDKDRDTVCETVDLNSQETVKVQIHGRASEYITDVHLNDLSTPQILPSNESVNPRLSASPPKSGNLWPGLTPPHKKAQSSSPKRKKQHKKYRSVISDIFDGTIISSVQCLTCDRVSVTLETFQDLSLPIPGKEDLAKLHSSSHPTIVKAGSCGEAYAPQGWIAFFMEYVKSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQKFPEILCIHLKRFRHELMFSTKISTHVSFPLEGLDLQPFLAKDSPAQIVTYDLLSVICHHGTASSGHYIAYCRNNLNNLWYEFDDQSVTEVSESTVQNAEAYVLFYRKSSEEAQRERRRISNLLNIMEPSLLQFYISRQWLNKFKTFAEPGPISNNDFLCIHGGIPPRKASYIEDLVLMLPQNIWDNLYSRYGGGPAVNHLYICHTCQIEAEKIEKRRKTELEIFIRLNRAFQEEDSPATFYCISMQWFREWESFVKGKDGDPPGPIDNTKIAVTKCGNVMLKQGADSGQISEETWNFLQSIYGGGPEVILRPPVVHVDPDALQAEEKIEVETRSL

Target ORF information:

RefSeq Version	XM_006233493.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 33 (Usp33), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233493.2

ORF Insert Sequence:

ATGACGACTT TTCGAAATCA TTGTCCACAT TTGGATTCAG TTGGTGAAAT AACAAAAGAG 
GATTTGATAC AAAAATCTCA TGGTGCTTGT CAGGACTGTA AAGTCAGAGG ACCAAACCTG 
TGGGCATGCC TAGAGAATAG GTGTTCCTAT GTTGGCTGCG GGGAGTCACA AGATCATAGC 
ACCATACACT CGAAGAAGAC AAAGCATTAT CTAACTGTGA ACCTCACCAC CCTTCGAGTA 
TGGTGTTATG CTTGCAGCAA AGAAGTATTC TTGGATAGAA AATTAGGAAC TCCTCCTTCA 
TTACCTCATG TAAGACAGCC TCAACAAACA CAAGAAAACA GTGTCCAGGA TTTTAAAATA 
CCCAGTAATA CAGCATTGAA AACCCCTCTG GTTGCTGTAT TTGAAGATCT GGATATAGAA 
GTGGAAGAGG AAGATGAGCT GAAGGCTAGA GGCCTGACAG GTTTGAAAAA CATTGGAAAT 
ACTTGCTATA TGAATGCCGC TCTGCAAGCT CTCTCTAACT GCCCACCTTT GACACAGTTC 
TTTCTTGATT GTGGAGGACT GGCTAGAACA GATAAGAAAC CAGCCATTTG TAAAAGTTAT 
CTCAAACTAA TGACAGAATT GTGGCACAAG AGCAGGCCAG GATCTGTTGT GCCTGCTAAT 
CTGTTCCAAG GAATTAAAAC TGTAAATCCA ACTTTTCGAG GCTATTCTCA GCAGGATGCC 
CAGGAATTCC TTCGCTGTTT AATGGATCTG CTTCATGAAG AGTTGAAGGA GCAGGTCATG 
GAAATAGAGG AAGAGCCTCA AGCACTAACT TCTGAGGAGA CAGTGGAGGA AGAGAAGAGC 
CAGTCAGATG TGGATTTTCA GTCCTGCGAG TCATGTAGCA GCAGCAGTGA GAAAGGGGAA 
AATGAGAGTG TCTCTAAGGG CGGCCCTGAA GACAGCACTG AGACCACCAT GCTCATTCAG 
GATGAGGAGG ACCTAGAGAT GGCCAAAGAC TGGCAAAAAG AGAAGATGTG CAACAAGAGC 
AACAAAGCAG ATTCTGACGG AGAACCGGAT AAAGACAGAG ACACGGTGTG TGAAACAGTT 
GACCTAAACA GCCAGGAGAC CGTCAAGGTG CAGATACACG GCAGAGCCTC AGAATATATC 
ACTGATGTCC ATTTGAATGA CCTATCTACA CCACAGATCC TTCCATCAAA TGAAAGTGTT 
AATCCACGTT TATCAGCAAG TCCTCCTAAG TCAGGCAATC TGTGGCCAGG ATTGACACCT 
CCACACAAGA AAGCTCAGTC TTCATCTCCA AAGAGGAAAA AGCAGCATAA GAAGTACAGA 
AGTGTCATCT CTGACATATT TGATGGGACA ATCATTAGTT CAGTACAGTG TCTGACGTGT 
GATAGGGTGT CTGTAACCCT CGAGACCTTT CAGGATCTGT CCTTGCCGAT TCCTGGCAAG 
GAAGACCTGG CTAAGCTGCA CTCCTCCAGT CACCCAACTA TAGTCAAAGC AGGGTCGTGT 
GGCGAAGCCT ACGCTCCACA GGGGTGGATA GCTTTCTTCA TGGAGTACGT GAAGAGCTGG 
TTTTGGGGTC CAGTAGTAAC CTTGCAAGAT TGTCTTGCTG CCTTCTTCGC CAGAGATGAA 
CTTAAAGGTG ATAATATGTA CAGTTGTGAA AAATGCAAAA AGTTGAGGAA TGGAGTAAAG 
TTTTGTAAAG TACAAAAGTT TCCTGAGATT TTGTGTATCC ACCTTAAAAG ATTTCGACAT 
GAACTGATGT TTTCCACCAA AATTAGCACC CATGTTTCCT TCCCCCTGGA AGGCCTGGAC 
CTTCAGCCAT TTCTTGCAAA GGATAGCCCA GCTCAGATCG TGACATATGA TCTCCTGTCA 
GTCATCTGTC ATCATGGGAC TGCAAGTAGT GGGCACTACA TTGCCTACTG CAGAAACAAT 
TTAAATAACC TGTGGTATGA GTTTGATGAC CAGAGCGTCA CTGAAGTTTC AGAGTCCACC 
GTACAGAATG CCGAAGCCTA CGTCCTTTTC TACAGGAAGA GCAGTGAAGA GGCACAAAGG 
GAGAGGCGAA GGATATCCAA TTTGTTGAAC ATAATGGAGC CAAGCCTCCT TCAGTTCTAT 
ATATCTCGAC AGTGGTTGAA TAAATTTAAG ACCTTTGCTG AACCTGGCCC TATTTCAAAT 
AATGACTTTC TCTGTATTCA TGGAGGTATT CCTCCACGAA AAGCAAGTTA TATTGAAGAC 
TTAGTTTTGA TGCTGCCTCA GAACATTTGG GATAACCTCT ACAGCAGGTA TGGAGGAGGG 
CCTGCTGTCA ACCACCTCTA CATTTGTCAC ACTTGCCAAA TTGAGGCAGA GAAGATTGAA 
AAACGAAGAA AAACTGAATT GGAAATTTTT ATTCGGCTCA ACAGAGCATT TCAAGAGGAA 
GACTCTCCAG CTACTTTTTA CTGTATCAGC ATGCAGTGGT TTAGAGAATG GGAGAGTTTT 
GTAAAGGGTA AGGATGGAGA TCCTCCAGGT CCAATAGACA ACACTAAAAT TGCAGTCACT 
AAGTGTGGTA ATGTGATGCT CAAGCAAGGA GCAGATTCTG GTCAAATTTC AGAAGAAACA 
TGGAATTTCC TGCAATCTAT ATATGGTGGG GGACCTGAAG TTATCCTCCG ACCTCCAGTT 
GTTCATGTTG ACCCTGATGC ACTACAAGCA GAGGAAAAGA TTGAAGTAGA AACTCGCTCT 
TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.
Biological trace element research174(2)377-386(2016 Dec)
Zhang X,Jiang Y,Han W,Liu A,Xie X,Han C,Fan C,Wang H,Zhang H,Ding S,Shan Z,Teng W

The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.
Nature cell biology15(10)1220-30(2013 Oct)
Simicek M,Lievens S,Laga M,Guzenko D,Aushev VN,Kalev P,Baietti MF,Strelkov SV,Gevaert K,Tavernier J,Sablina AA

USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110.
Nature495(7440)255-9(2013 Mar)
Li J,D'Angiolella V,Seeley ES,Kim S,Kobayashi T,Fu W,Campos EI,Pagano M,Dynlacht BD

Isoform-specific localization of the deubiquitinase USP33 to the Golgi apparatus.
Traffic (Copenhagen, Denmark)12(11)1563-74(2011 Nov)
Thorne C,Eccles RL,Coulson JM,Urb? S,Clague MJ

Deubiquitinating enzyme USP33/VDU1 is required for Slit signaling in inhibiting breast cancer cell migration.
Proceedings of the National Academy of Sciences of the United States of America106(34)14530-5(2009 Aug)
Yuasa-Kawada J,Kinoshita-Kawada M,Rao Y,Wu JY

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Usp33 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

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電話

FAX

オンラインフォーム