usp33 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	usp33
Entrez Gene ID	406508
Full Name	ubiquitin specific peptidase 33
Synonyms	zgc:77308
General protein information	Preferred Names ubiquitin specific peptidase 33 Names ubiquitin carboxyl-terminal hydrolase 33 deubiquitinating enzyme 33 ubiquitin thioesterase 33 ubiquitin thiolesterase 33 ubiquitin-specific-processing protease 33
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_213227.1	NP_998392.1	ubiquitin carboxyl-terminal hydrolase 33

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	USP33	XP_003308213.1
	LOC710693	XP_002808266.1
Xenopus tropicalis (tropical clawed frog)	usp33	NP_001016228.1
Mus musculus (house mouse)	Usp33	NP_573510.2
Homo sapiens (human)	USP33	NP_055832.3
Rattus norvegicus (Norway rat)	Usp33	NP_001178023.1
Danio rerio (zebrafish)	usp33	NP_998392.1
Bos taurus (cattle)	USP33	NP_001094609.1
Canis lupus familiaris (dog)	USP33	XP_005622106.1
Gallus gallus (chicken)	USP33	XP_004936727.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.
Kok FO, Shin M, Ni CW, Gupta A, Grosse AS, van Impel A, Kirchmaier BC, Peterson-Maduro J, Kourkoulis G, Male I, DeSantis DF, Sheppard-Tindell S, Ebarasi L, Betsholtz C, Schulte-Merker S, Wolfe SA, Lawson ND
Developmental cell32(1)97-108(2015 Jan)

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
Tse WK, Eisenhaber B, Ho SH, Ng Q, Eisenhaber F, Jiang YJ
BMC genomics10637(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following usp33 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the usp33 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa37917	NM_213227.1 Latest version!	Danio rerio ubiquitin specific peptidase 33 (usp33), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

usp33 ( NM_213227.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998392.1 Danio rerio ubiquitin specific peptidase 33 (usp33), mRNA.

Price & Availability↑

CloneID	ODa37917
Clone ID Related Accession (Same CDS sequence)	NM_213227.1
Accession Version	NM_213227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ubiquitin carboxyl-terminal hydrolase 33
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC066557.1. ##Evidence-Data-START## Transcript exon combination :: BC066557.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGGAG TGTCCAGCAA TGACTGTCCA CACTTGGAGT GTGTGGGGGA GATCACCAAA 
GAAGAACTCA TCCAGAAGTC TCATGGTCAG TGTCAGGACT GCAAAGTTGG GGGACCAAAT 
CTTTGGGCAT GTCTAGAGAA TGGCTGTTCA TATGTTGGCT GTGGAGAATC GCATGCGGAT 
CACAGCACTG TTCACTCTCA GGAGACGCGG CACAACTTGA CGGTCAATCT GACCACTTTG 
AGAGTGTGGT GTTATGCCTG CACCAAGGAG GTTTTCCTGG AAAGGAAACT GGGTCCACAT 
AAACAGTTGC CCAATGCTGC CAAAGCAGTA TCCCCAGTGC AGACTCCCTG CCAGGACCTC 
AATACTCCTG GGAGTCCTAC TTCCCTAAGA GTTCCTTCAG CAGGAACATG TGATGACCTG 
GACATGGAGA CAGAGGAAGA AGATGAACTG AGGACTAGAG GTCTGACTGG ACTAAAGAAC 
ATTGGCAATA CGTGCTACAT GAATGCAGCT TTGCAGGCAC TCTCCAATTG TCCTCCGCTG 
ACCCAGTTCT TTTTGGAGTG CGGTGGTCTG GTGAAGACAG ACAAGAAGCC AGCGCTATGC 
AAGAGCTATC AGAAACTCGT CACAGACCTG TGGCACAAAA ACAGGAACTC CTATGTTGTT 
CCCACAAATC TATTCCAGGG CATTAAGGCT GTGAACCCAA TGTTTCGTGG TTACTCCCAA 
CAGGACTCTC AGGAATTCCT GCGTTGTTTG ATGGACCAAC TCCACGAGGA GCTCAAGGAG 
CTCATTCCTG AACCCGAGGA CCCAAATCAG GCTGTGGCCA TGGACGACAG TCCCGACGAG 
GACAACCACA GCCAATCAGA TGACTTCCAG TCCTGCGAGT CTTGCGGCAG CAGTGATCGT 
GCTGATAACG AAGGCCCCAG AGTCCCAGAA GACATCAACG AAGCTGAGAT GCTGATGCCT 
GAACAGAATC AAAACAACCG AGATTGGCAG AAAGAGAAGA ATCTCATCAA CAACCTGTAC 
CGTGCCGGTT CCCATGGCGA CCTGGATAAA GACGTGGACA CGACCAGTGA TTCAAGGCCA 
ATCATCAGCA GCCAAGGAGC AATCAAAGCT CAGGGGCGAA CTTCAGATTC TGAAATCCAG 
GTCAGCAGTA CTGTCAGACC GCAGAGTCCC ACTGGAAATG AGGGCATCAC ATCTAGACTG 
TCCAGCAGCC CCCCAAAATC CTCAGCATGG CCAAACCTGA GCTCTACTCA CAAGAAAGTA 
CCCATGTTCA CCCCAACAAA GACCAAGCGG CAGAGGAAAT ACCACAGCAT CATTTCTGAA 
GTGTTTGATG GCACAATTGT CAGCTCAGTT CAGTGCCTAA CCTGTGATAG GGTTTCGGTG 
ACTTTGGAGA ACTTCCAGGA CATCTCTCTG CCCATCCCAG GGAAAGAGGA CCTTGCCAAG 
CTGCACTCGT CCTCCCACCA GACGGCTCTG GTCAAGGCTG GCTCTTGTGG TGAAGCATAC 
GCCCCTCAAG GCTGGATTGC TTTTGTCATG GAATATATCA AGAGCTGGTT CTGGGGACCT 
GTGGTGACAC TGCAGGATTG TCTGGCAGCC TTTTTTGCCA GGGATGAACT AAAAGGAGAC 
AACATGTACA GCTGTGAAAA ATGCAAGAAA TTACGGAATG GGGTCAAATT CTGCAAAGTT 
CAGAGTTTGC CAGAGATTTT GTGCATTCAT CTCAAGCGCT TCAGACATGA ACTGATGTTC 
TCCACCAAAA TCGGCACTCA TGTGTCATTC CCTCTGGAAG GCCTTGAAAT GCAGCCCTTT 
TTGGCCAAGG ACAGCTCTGC CCTGACCACC ACCTATGACC TGCTTTCAGT CATCTGTCAC 
CATGGCACTG CCAGCAGTGG CCACTATATT GCCTATTGTC GGAATGAGCT GAACCAACTG 
TGGTACGAGT TTGACGACCA GAGCGTTACT GAAGTGTCAG AGTCCTGTGT TCAAAACGCT 
GAGGCATATG TGCTCTTTTA CAAGAAGAGT AATGATGAGA CTCAGAAGGA AAGGAGGAAG 
GTGACCAGTC TGTTCAACAT GATGGAGCCA AGCCTCCTGC AGTTCTACGT CTCCCGCCAG 
TGGCTGAACA AGTTCAAAAC TTTTGCTGAG CCAGGACCCA TTTCCAACCA TGACTTCCTG 
TGTGCCCATG GAGGTATTCC GCCAAATAAG GCCACATACA TTGATGACCT TGTTTTGATG 
ATTCCCCAAA ACGTGTGGGA CCATCTCTAT AGCAGGTATG GAGGCGGACC TGCCGTTAAT 
CACTTGTATG TCTGCCACAC CTGCCAGAAT GAGATTGAGA AGCTGGAGAA ACGACGCAAG 
AATGAACTGG ACATGTTTGT TCGGCTCAAT AAGGCGTTTC AGGAGGAGGA GTCTCCTGTG 
GTGATATACT GTATCAGCAT GCAGTGGTTC CGGGAATGGG AAAGCTTTGT CAAAGGCAAG 
GACATTGACC CACCGGGGCC AATTGATAAC TCCAAGATTG CTGTAAATAA AAATGGACAC 
ATCACATTGA AGCCAGGTGC TGATTCAGGT CAAATATCCG AAGAGACCTG GAACTTCCTC 
CATAACATTC ACGGAGGAGG GCCTGTAGTG ACCGTGCGGC CCAGCGTCAG CCATCAAGAG 
TCCGAGACTT CACAGTCAGA GGAGAAGATC GAAGTGGAGA CGCGCACAGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998392.1
CDS	194..2887
Translation	MSGVSSNDCPHLECVGEITKEELIQKSHGQCQDCKVGGPNLWACLENGCSYVGCGESHADHSTVHSQETRHNLTVNLTTLRVWCYACTKEVFLERKLGPHKQLPNAAKAVSPVQTPCQDLNTPGSPTSLRVPSAGTCDDLDMETEEEDELRTRGLTGLKNIGNTCYMNAALQALSNCPPLTQFFLECGGLVKTDKKPALCKSYQKLVTDLWHKNRNSYVVPTNLFQGIKAVNPMFRGYSQQDSQEFLRCLMDQLHEELKELIPEPEDPNQAVAMDDSPDEDNHSQSDDFQSCESCGSSDRADNEGPRVPEDINEAEMLMPEQNQNNRDWQKEKNLINNLYRAGSHGDLDKDVDTTSDSRPIISSQGAIKAQGRTSDSEIQVSSTVRPQSPTGNEGITSRLSSSPPKSSAWPNLSSTHKKVPMFTPTKTKRQRKYHSIISEVFDGTIVSSVQCLTCDRVSVTLENFQDISLPIPGKEDLAKLHSSSHQTALVKAGSCGEAYAPQGWIAFVMEYIKSWFWGPVVTLQDCLAAFFARDELKGDNMYSCEKCKKLRNGVKFCKVQSLPEILCIHLKRFRHELMFSTKIGTHVSFPLEGLEMQPFLAKDSSALTTTYDLLSVICHHGTASSGHYIAYCRNELNQLWYEFDDQSVTEVSESCVQNAEAYVLFYKKSNDETQKERRKVTSLFNMMEPSLLQFYVSRQWLNKFKTFAEPGPISNHDFLCAHGGIPPNKATYIDDLVLMIPQNVWDHLYSRYGGGPAVNHLYVCHTCQNEIEKLEKRRKNELDMFVRLNKAFQEEESPVVIYCISMQWFREWESFVKGKDIDPPGPIDNSKIAVNKNGHITLKPGADSGQISEETWNFLHNIHGGGPVVTVRPSVSHQESETSQSEEKIEVETRTV

Target ORF information:

RefSeq Version	NM_213227.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ubiquitin specific peptidase 33 (usp33), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213227.1

ORF Insert Sequence:

ATGTCTGGAG TGTCCAGCAA TGACTGTCCA CACTTGGAGT GTGTGGGGGA GATCACCAAA 
GAAGAACTCA TCCAGAAGTC TCATGGTCAG TGTCAGGACT GCAAAGTTGG GGGACCAAAT 
CTTTGGGCAT GTCTAGAGAA TGGCTGTTCA TATGTTGGCT GTGGAGAATC GCATGCGGAT 
CACAGCACTG TTCACTCTCA GGAGACGCGG CACAACTTGA CGGTCAATCT GACCACTTTG 
AGAGTGTGGT GTTATGCCTG CACCAAGGAG GTTTTCCTGG AAAGGAAACT GGGTCCACAT 
AAACAGTTGC CCAATGCTGC CAAAGCAGTA TCCCCAGTGC AGACTCCCTG CCAGGACCTC 
AATACTCCTG GGAGTCCTAC TTCCCTAAGA GTTCCTTCAG CAGGAACATG TGATGACCTG 
GACATGGAGA CAGAGGAAGA AGATGAACTG AGGACTAGAG GTCTGACTGG ACTAAAGAAC 
ATTGGCAATA CGTGCTACAT GAATGCAGCT TTGCAGGCAC TCTCCAATTG TCCTCCGCTG 
ACCCAGTTCT TTTTGGAGTG CGGTGGTCTG GTGAAGACAG ACAAGAAGCC AGCGCTATGC 
AAGAGCTATC AGAAACTCGT CACAGACCTG TGGCACAAAA ACAGGAACTC CTATGTTGTT 
CCCACAAATC TATTCCAGGG CATTAAGGCT GTGAACCCAA TGTTTCGTGG TTACTCCCAA 
CAGGACTCTC AGGAATTCCT GCGTTGTTTG ATGGACCAAC TCCACGAGGA GCTCAAGGAG 
CTCATTCCTG AACCCGAGGA CCCAAATCAG GCTGTGGCCA TGGACGACAG TCCCGACGAG 
GACAACCACA GCCAATCAGA TGACTTCCAG TCCTGCGAGT CTTGCGGCAG CAGTGATCGT 
GCTGATAACG AAGGCCCCAG AGTCCCAGAA GACATCAACG AAGCTGAGAT GCTGATGCCT 
GAACAGAATC AAAACAACCG AGATTGGCAG AAAGAGAAGA ATCTCATCAA CAACCTGTAC 
CGTGCCGGTT CCCATGGCGA CCTGGATAAA GACGTGGACA CGACCAGTGA TTCAAGGCCA 
ATCATCAGCA GCCAAGGAGC AATCAAAGCT CAGGGGCGAA CTTCAGATTC TGAAATCCAG 
GTCAGCAGTA CTGTCAGACC GCAGAGTCCC ACTGGAAATG AGGGCATCAC ATCTAGACTG 
TCCAGCAGCC CCCCAAAATC CTCAGCATGG CCAAACCTGA GCTCTACTCA CAAGAAAGTA 
CCCATGTTCA CCCCAACAAA GACCAAGCGG CAGAGGAAAT ACCACAGCAT CATTTCTGAA 
GTGTTTGATG GCACAATTGT CAGCTCAGTT CAGTGCCTAA CCTGTGATAG GGTTTCGGTG 
ACTTTGGAGA ACTTCCAGGA CATCTCTCTG CCCATCCCAG GGAAAGAGGA CCTTGCCAAG 
CTGCACTCGT CCTCCCACCA GACGGCTCTG GTCAAGGCTG GCTCTTGTGG TGAAGCATAC 
GCCCCTCAAG GCTGGATTGC TTTTGTCATG GAATATATCA AGAGCTGGTT CTGGGGACCT 
GTGGTGACAC TGCAGGATTG TCTGGCAGCC TTTTTTGCCA GGGATGAACT AAAAGGAGAC 
AACATGTACA GCTGTGAAAA ATGCAAGAAA TTACGGAATG GGGTCAAATT CTGCAAAGTT 
CAGAGTTTGC CAGAGATTTT GTGCATTCAT CTCAAGCGCT TCAGACATGA ACTGATGTTC 
TCCACCAAAA TCGGCACTCA TGTGTCATTC CCTCTGGAAG GCCTTGAAAT GCAGCCCTTT 
TTGGCCAAGG ACAGCTCTGC CCTGACCACC ACCTATGACC TGCTTTCAGT CATCTGTCAC 
CATGGCACTG CCAGCAGTGG CCACTATATT GCCTATTGTC GGAATGAGCT GAACCAACTG 
TGGTACGAGT TTGACGACCA GAGCGTTACT GAAGTGTCAG AGTCCTGTGT TCAAAACGCT 
GAGGCATATG TGCTCTTTTA CAAGAAGAGT AATGATGAGA CTCAGAAGGA AAGGAGGAAG 
GTGACCAGTC TGTTCAACAT GATGGAGCCA AGCCTCCTGC AGTTCTACGT CTCCCGCCAG 
TGGCTGAACA AGTTCAAAAC TTTTGCTGAG CCAGGACCCA TTTCCAACCA TGACTTCCTG 
TGTGCCCATG GAGGTATTCC GCCAAATAAG GCCACATACA TTGATGACCT TGTTTTGATG 
ATTCCCCAAA ACGTGTGGGA CCATCTCTAT AGCAGGTATG GAGGCGGACC TGCCGTTAAT 
CACTTGTATG TCTGCCACAC CTGCCAGAAT GAGATTGAGA AGCTGGAGAA ACGACGCAAG 
AATGAACTGG ACATGTTTGT TCGGCTCAAT AAGGCGTTTC AGGAGGAGGA GTCTCCTGTG 
GTGATATACT GTATCAGCAT GCAGTGGTTC CGGGAATGGG AAAGCTTTGT CAAAGGCAAG 
GACATTGACC CACCGGGGCC AATTGATAAC TCCAAGATTG CTGTAAATAA AAATGGACAC 
ATCACATTGA AGCCAGGTGC TGATTCAGGT CAAATATCCG AAGAGACCTG GAACTTCCTC 
CATAACATTC ACGGAGGAGG GCCTGTAGTG ACCGTGCGGC CCAGCGTCAG CCATCAAGAG 
TCCGAGACTT CACAGTCAGA GGAGAAGATC GAAGTGGAGA CGCGCACAGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.
Developmental cell32(1)97-108(2015 Jan)
Kok FO,Shin M,Ni CW,Gupta A,Grosse AS,van Impel A,Kirchmaier BC,Peterson-Maduro J,Kourkoulis G,Male I,DeSantis DF,Sheppard-Tindell S,Ebarasi L,Betsholtz C,Schulte-Merker S,Wolfe SA,Lawson ND

Genome-wide loss-of-function analysis of deubiquitylating enzymes for zebrafish development.
BMC genomics10637(2009 Dec)
Tse WK,Eisenhaber B,Ho SH,Ng Q,Eisenhaber F,Jiang YJ