Ppp1r14b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r14b
Entrez Gene ID	259225
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 14B
Synonyms	PHI-1
General protein information	Preferred Names protein phosphatase 1, regulatory (inhibitor) subunit 14B Names protein phosphatase 1 regulatory subunit 14B phosphatase holoenzyme inhibitor 1 protein phosphatase 1, regulatory (inhibitor) 5 subunit 14B ubiquitous PKC-potentiated PP1 inhibitor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	inhibitor for protein type-1 phosphatase (PP1) and myosin light-chain phosphatase (MLCP); substrate of protein kinase C (PKC) [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172045.1	NP_742042.1	protein phosphatase 1 regulatory subunit 14B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP1R14B	XP_533244.2
Mus musculus (house mouse)	Ppp1r14b	NP_032915.2
Rattus norvegicus (Norway rat)	Ppp1r14b	NP_742042.1
Danio rerio (zebrafish)	ppp1r14bb	NP_001002692.1
Danio rerio (zebrafish)	ppp1r14ba	NP_001039318.1
Homo sapiens (human)	PPP1R14B	NP_619634.1
Pan troglodytes (chimpanzee)	PPP1R14B	XP_003313096.1
Xenopus tropicalis (tropical clawed frog)	ppp1r14b	NP_001016307.1

Gene Ontology
Bibliography

Related articles in PubMed

Migration and retraction of endothelial and epithelial cells require PHI-1, a specific protein-phosphatase-1 inhibitor protein.
Tountas NA, Brautigan DL
Journal of cell science117(Pt 24)5905-12(2004 Nov)

GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA.
Liu QR, Zhang PW, Lin Z, Li QF, Woods AS, Troncoso J, Uhl GR
The Biochemical journal377(Pt 1)171-81(2004 Jan)

Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase.
Deng JT, Sutherland C, Brautigan DL, Eto M, Walsh MP
The Biochemical journal367(Pt 2)517-24(2002 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PHI-1 participates in regulatory events at the trailing edge of migrating cells and modulates retraction of endothelial and epithelial cells
Title: Migration and retraction of endothelial and epithelial cells require PHI-1, a specific protein-phosphatase-1 inhibitor protein.

ILK may activate smooth-muscle contraction both directly, via phosphorylation of myosin, and indirectly, via phosphorylation and activation of CPI-17 and PHI-1, leading to inhibition of myosin light-chain phosphatase.
Title: Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase.

The following Ppp1r14b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r14b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02852	NM_172045.1 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14B (Ppp1r14b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r14b ( NM_172045.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742042.1 Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14B (Ppp1r14b), mRNA.

Price & Availability↑

CloneID	ORa02852
Clone ID Related Accession (Same CDS sequence)	NM_172045.1
Accession Version	NM_172045.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 14B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY122323.1. On Jun 25, 2006 this sequence version replaced XM_001074638.1. ##Evidence-Data-START## Transcript exon combination :: AY122323.1, AW918183.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGATA GCGGCCCCGC GGGGGGCGCG GCGTTGGCTG CCCCGGCCCC CGGACCGGGT 
AGTGGCAGCA CAGGGCCCCG AGTCTACTTC CAGAGTCCCC CTGGGGCCGC AGGCGAAGGC 
CCGGGGGGCG CGGATGATGA TGGCCCGGTG AGACGGCAAG GGAAGGTCAC CGTCAAGTAC 
GACCGCAAGG AGCTGCGGAA GCGCCTCAAC CTGGAGGAGT GGATTTTAGA ACAGCTCACG 
CGACTCTACG ACTGCCAGGA GGAGGAGATC CCAGAGCTGG AGATCGATGT GGATGAACTC 
TTGGACATGG AAAGTGATGA TACTCGGGCT GCTAGAGTCA AGGAGCTGCT AGTTGACTGT 
TACAAACCCA CTGAGGCCTT CATCTCTGGC CTGCTGGACA AGATCCGGGG CATGCAGAAG 
CTGAGCACAC CCCAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742042.1
CDS	9..452
Translation	MADSGPAGGAALAAPAPGPGSGSTGPRVYFQSPPGAAGEGPGGADDDGPVRRQGKVTVKYDRKELRKRLNLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDTRAARVKELLVDCYKPTEAFISGLLDKIRGMQKLSTPQKK

Target ORF information:

RefSeq Version	NM_172045.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 14B (Ppp1r14b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172045.1

ORF Insert Sequence:

ATGGCGGATA GCGGCCCCGC GGGGGGCGCG GCGTTGGCTG CCCCGGCCCC CGGACCGGGT 
AGTGGCAGCA CAGGGCCCCG AGTCTACTTC CAGAGTCCCC CTGGGGCCGC AGGCGAAGGC 
CCGGGGGGCG CGGATGATGA TGGCCCGGTG AGACGGCAAG GGAAGGTCAC CGTCAAGTAC 
GACCGCAAGG AGCTGCGGAA GCGCCTCAAC CTGGAGGAGT GGATTTTAGA ACAGCTCACG 
CGACTCTACG ACTGCCAGGA GGAGGAGATC CCAGAGCTGG AGATCGATGT GGATGAACTC 
TTGGACATGG AAAGTGATGA TACTCGGGCT GCTAGAGTCA AGGAGCTGCT AGTTGACTGT 
TACAAACCCA CTGAGGCCTT CATCTCTGGC CTGCTGGACA AGATCCGGGG CATGCAGAAG 
CTGAGCACAC CCCAGAAGAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Migration and retraction of endothelial and epithelial cells require PHI-1, a specific protein-phosphatase-1 inhibitor protein.
Journal of cell science117(Pt 24)5905-12(2004 Nov)
Tountas NA,Brautigan DL

GBPI, a novel gastrointestinal- and brain-specific PP1-inhibitory protein, is activated by PKC and inactivated by PKA.
The Biochemical journal377(Pt 1)171-81(2004 Jan)
Liu QR,Zhang PW,Lin Z,Li QF,Woods AS,Troncoso J,Uhl GR

Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase.
The Biochemical journal367(Pt 2)517-24(2002 Oct)
Deng JT,Sutherland C,Brautigan DL,Eto M,Walsh MP