PPP1R14B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R14B
Entrez Gene ID	26472
Full Name	protein phosphatase 1 regulatory inhibitor subunit 14B
Synonyms	PHI-1,PLCB3N,PNG,SOM172
General protein information	Preferred Names protein phosphatase 1 regulatory inhibitor subunit 14B Names protein phosphatase 1 regulatory subunit 14B phospholipase C beta-3 neighboring gene protein phospholipase C-beta-3 neighbouring gene protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.1
Disorder MIM:	601140

mRNA and Protein(s)

mRNA	Protein	Name
NM_138689.2	NP_619634.1	protein phosphatase 1 regulatory subunit 14B
NM_138689.3	NP_619634.1	protein phosphatase 1 regulatory subunit 14B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP1R14B	XP_533244.2
Mus musculus (house mouse)	Ppp1r14b	NP_032915.2
Rattus norvegicus (Norway rat)	Ppp1r14b	NP_742042.1
Danio rerio (zebrafish)	ppp1r14bb	NP_001002692.1
Danio rerio (zebrafish)	ppp1r14ba	NP_001039318.1
Homo sapiens (human)	PPP1R14B	NP_619634.1
Pan troglodytes (chimpanzee)	PPP1R14B	XP_003313096.1
Xenopus tropicalis (tropical clawed frog)	ppp1r14b	NP_001016307.1

Gene Ontology
Bibliography

Related articles in PubMed

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Bailey SD, Xie C, Do R, Montpetit A, Diaz R, Mohan V, Keavney B, Yusuf S, Gerstein HC, Engert JC, Anand S,
Diabetes care33(10)2250-3(2010 Oct)

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
Talmud PJ, Drenos F, Shah S, Shah T, Palmen J, Verzilli C, Gaunt TR, Pallas J, Lovering R, Li K, Casas JP, Sofat R, Kumari M, Rodriguez S, Johnson T, Newhouse SJ, Dominiczak A, Samani NJ, Caulfield M, Sever P, Stanton A, Shields DC, Padmanabhan S, Melander O, Hastie C, Delles C, Ebrahim S, Marmot MG, Smith GD, Lawlor DA, Munroe PB, Day IN, Kivimaki M, Whittaker J, Humphries SE, Hingorani AD, , ,
American journal of human genetics85(5)628-42(2009 Nov)

A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
Beausoleil SA, Vill?n J, Gerber SA, Rush J, Gygi SP
Nature biotechnology24(10)1285-92(2006 Oct)

Transcriptome analysis of human gastric cancer.
Oh JH, Yang JO, Hahn Y, Kim MR, Byun SS, Jeon YJ, Kim JM, Song KS, Noh SM, Kim S, Yoo HS, Kim YS, Kim NS
Mammalian genome : official journal of the International Mammalian Genome Society16(12)942-54(2005 Dec)

Migration and retraction of endothelial and epithelial cells require PHI-1, a specific protein-phosphatase-1 inhibitor protein.
Tountas NA, Brautigan DL
Journal of cell science117(Pt 24)5905-12(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

PHI-1 participates in regulatory events at the trailing edge of migrating cells and modulates retraction of endothelial and epithelial cells
Title: Migration and retraction of endothelial and epithelial cells require PHI-1, a specific protein-phosphatase-1 inhibitor protein.

Whereas CPI-17 staining was diffuse, PHI-1 was concentrated along the cell membrane in distinct foci. The common tissue distribution but different cellular localization of PHI-1 and CPI-17 suggest distinctive physiological roles for these PP1 inhibitors.
Title: Juxtamembrane localization of the protein phosphatase-1 inhibitor protein PHI-1 in smooth muscle cells.

An inhibitory phosphoprotein that inhibits the myosin- and glycogen-associated holoenzyme versions of protein type-1 phosphatase (PP1) as well as the monomeric catalytic subunit of PP1 in multiple tissues.
Title: A novel phosphoprotein inhibitor of protein type-1 phosphatase holoenzymes.

The following PPP1R14B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R14B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30908	NM_138689.3 Latest version!	Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 14B (PPP1R14B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30908	NM_138689.2	Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 14B (PPP1R14B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R14B ( NM_138689.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_619634.1 Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 14B (PPP1R14B), mRNA.

Price & Availability↑

CloneID	OHu30908
Clone ID Related Accession (Same CDS sequence)	NM_138689.2 , NM_138689.3
Accession Version	NM_138689.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 14B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BM847221.1, BC014522.2, BF155230.1 and BC071884.1. On or before Apr 3, 2005 this sequence version replaced XM_370630.2, NM_138689.1. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.489906.1, SRR1163658.175299.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACA GCGGCACCGC GGGGGGCGCG GCGTTGGCGG CCCCGGCCCC CGGGCCGGGC 
AGTGGCGGCC CAGGACCACG CGTCTACTTT CAGAGCCCCC CCGGGGCCGC AGGAGAGGGC 
CCGGGCGGGG CGGACGATGA GGGCCCAGTG AGGCGCCAAG GGAAGGTCAC CGTCAAGTAT 
GACCGCAAGG AGCTACGGAA GCGCCTCAAC CTAGAGGAGT GGATCCTGGA GCAGCTCACG 
CGCCTCTACG ACTGCCAGGA AGAGGAGATC CCAGAACTGG AGATTGACGT GGATGAGCTC 
CTGGACATGG AGAGTGACGA TGCCCGGGCT GCCAGGGTCA AGGAGCTGCT GGTTGACTGT 
TACAAACCCA CAGAGGCCTT CATTTCTGGC CTGCTGGACA AGATCCGGGG CATGCAGAAG 
CTGAGCACAC CCCAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619634.1
CDS	269..712
Translation	MADSGTAGGAALAAPAPGPGSGGPGPRVYFQSPPGAAGEGPGGADDEGPVRRQGKVTVKYDRKELRKRLNLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDARAARVKELLVDCYKPTEAFISGLLDKIRGMQKLSTPQKK

Target ORF information:

RefSeq Version	NM_138689.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 14B (PPP1R14B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138689.2

ORF Insert Sequence:

ATGGCGGACA GCGGCACCGC GGGGGGCGCG GCGTTGGCGG CCCCGGCCCC CGGGCCGGGC 
AGTGGCGGCC CAGGACCACG CGTCTACTTT CAGAGCCCCC CCGGGGCCGC AGGAGAGGGC 
CCGGGCGGGG CGGACGATGA GGGCCCAGTG AGGCGCCAAG GGAAGGTCAC CGTCAAGTAT 
GACCGCAAGG AGCTACGGAA GCGCCTCAAC CTAGAGGAGT GGATCCTGGA GCAGCTCACG 
CGCCTCTACG ACTGCCAGGA AGAGGAGATC CCAGAACTGG AGATTGACGT GGATGAGCTC 
CTGGACATGG AGAGTGACGA TGCCCGGGCT GCCAGGGTCA AGGAGCTGCT GGTTGACTGT 
TACAAACCCA CAGAGGCCTT CATTTCTGGC CTGCTGGACA AGATCCGGGG CATGCAGAAG 
CTGAGCACAC CCCAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R14B ( NM_138689.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_619634.1 Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 14B (PPP1R14B), mRNA.

Price & Availability↑

CloneID	OHu30908
Clone ID Related Accession (Same CDS sequence)	NM_138689.2 , NM_138689.3
Accession Version	NM_138689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 14B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001453.6, BM847221.1, BC014522.2, BF155230.1 and BC071884.1. On Nov 23, 2018 this sequence version replaced NM_138689.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.215150.1, SRR1163658.87582.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000309318.8/ ENSP00000310117.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACA GCGGCACCGC GGGGGGCGCG GCGTTGGCGG CCCCGGCCCC CGGGCCGGGC 
AGTGGCGGCC CAGGACCACG CGTCTACTTT CAGAGCCCCC CCGGGGCCGC AGGAGAGGGC 
CCGGGCGGGG CGGACGATGA GGGCCCAGTG AGGCGCCAAG GGAAGGTCAC CGTCAAGTAT 
GACCGCAAGG AGCTACGGAA GCGCCTCAAC CTAGAGGAGT GGATCCTGGA GCAGCTCACG 
CGCCTCTACG ACTGCCAGGA AGAGGAGATC CCAGAACTGG AGATTGACGT GGATGAGCTC 
CTGGACATGG AGAGTGACGA TGCCCGGGCT GCCAGGGTCA AGGAGCTGCT GGTTGACTGT 
TACAAACCCA CAGAGGCCTT CATTTCTGGC CTGCTGGACA AGATCCGGGG CATGCAGAAG 
CTGAGCACAC CCCAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619634.1
CDS	271..714
Translation	MADSGTAGGAALAAPAPGPGSGGPGPRVYFQSPPGAAGEGPGGADDEGPVRRQGKVTVKYDRKELRKRLNLEEWILEQLTRLYDCQEEEIPELEIDVDELLDMESDDARAARVKELLVDCYKPTEAFISGLLDKIRGMQKLSTPQKK

Target ORF information:

RefSeq Version	NM_138689.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory inhibitor subunit 14B (PPP1R14B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138689.3

ORF Insert Sequence:

ATGGCGGACA GCGGCACCGC GGGGGGCGCG GCGTTGGCGG CCCCGGCCCC CGGGCCGGGC 
AGTGGCGGCC CAGGACCACG CGTCTACTTT CAGAGCCCCC CCGGGGCCGC AGGAGAGGGC 
CCGGGCGGGG CGGACGATGA GGGCCCAGTG AGGCGCCAAG GGAAGGTCAC CGTCAAGTAT 
GACCGCAAGG AGCTACGGAA GCGCCTCAAC CTAGAGGAGT GGATCCTGGA GCAGCTCACG 
CGCCTCTACG ACTGCCAGGA AGAGGAGATC CCAGAACTGG AGATTGACGT GGATGAGCTC 
CTGGACATGG AGAGTGACGA TGCCCGGGCT GCCAGGGTCA AGGAGCTGCT GGTTGACTGT 
TACAAACCCA CAGAGGCCTT CATTTCTGGC CTGCTGGACA AGATCCGGGG CATGCAGAAG 
CTGAGCACAC CCCAGAAGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Diabetes care33(10)2250-3(2010 Oct)
Bailey SD,Xie C,Do R,Montpetit A,Diaz R,Mohan V,Keavney B,Yusuf S,Gerstein HC,Engert JC,Anand S,

Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
American journal of human genetics85(5)628-42(2009 Nov)
Talmud PJ,Drenos F,Shah S,Shah T,Palmen J,Verzilli C,Gaunt TR,Pallas J,Lovering R,Li K,Casas JP,Sofat R,Kumari M,Rodriguez S,Johnson T,Newhouse SJ,Dominiczak A,Samani NJ,Caulfield M,Sever P,Stanton A,Shields DC,Padmanabhan S,Melander O,Hastie C,Delles C,Ebrahim S,Marmot MG,Smith GD,Lawlor DA,Munroe PB,Day IN,Kivimaki M,Whittaker J,Humphries SE,Hingorani AD,,,

A probability-based approach for high-throughput protein phosphorylation analysis and site localization.
Nature biotechnology24(10)1285-92(2006 Oct)
Beausoleil SA,Vill?n J,Gerber SA,Rush J,Gygi SP

Transcriptome analysis of human gastric cancer.
Mammalian genome : official journal of the International Mammalian Genome Society16(12)942-54(2005 Dec)
Oh JH,Yang JO,Hahn Y,Kim MR,Byun SS,Jeon YJ,Kim JM,Song KS,Noh SM,Kim S,Yoo HS,Kim YS,Kim NS

Migration and retraction of endothelial and epithelial cells require PHI-1, a specific protein-phosphatase-1 inhibitor protein.
Journal of cell science117(Pt 24)5905-12(2004 Nov)
Tountas NA,Brautigan DL