Sc5d cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sc5d
Entrez Gene ID	114100
Full Name	sterol-C5-desaturase
Synonyms	Sc5dl
General protein information	Preferred Names sterol-C5-desaturase Names lathosterol oxidase C-5 sterol desaturase delta(7)-sterol 5-desaturase delta(7)-sterol C5(6)-desaturase lathosterol 5-desaturase sterol-C5-desaturase (ERG3 delta-5-desaturase homolog, S. cerevisiae)-like sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog sterol-C5-desaturase (fungal ERG3, delta-5-desaturase)-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q22
Summary	human homolog is an enzyme involved in catalyzing the conversion of lathosterol into 7-dehydrocholesterol; involved in the synthesis of cholesterol [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017595406.1	XP_017450895.1	lathosterol oxidase isoform X1
NM_053642.2	NP_446094.1	lathosterol oxidase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SC5DL	XP_508825.1
Mus musculus (house mouse)	Sc5d	NP_766357.1
Gallus gallus (chicken)	SC5DL	XP_003642637.2
Eremothecium gossypii	AGOS_AFR151C	NP_985698.2
Schizosaccharomyces pombe (fission yeast)	erg32	NP_001018791.2
Canis lupus familiaris (dog)	SC5D	XP_853004.1
Rattus norvegicus (Norway rat)	Sc5d	NP_446094.1
Kluyveromyces lactis	KLLA0F08855g	XP_455482.1
Arabidopsis thaliana (thale cress)	AT3G02590	NP_186908.1
Oryza sativa (rice)	Os01g0134500	NP_001041944.1
Xenopus tropicalis (tropical clawed frog)	LOC100490845	XP_002941684.1
Homo sapiens (human)	SC5D	NP_001020127.1
Danio rerio (zebrafish)	sc5d	NP_001004630.1
Saccharomyces cerevisiae (baker's yeast)	ERG3	NP_013157.1
Schizosaccharomyces pombe (fission yeast)	erg31	NP_593135.1
	SC5DL	XP_001107388.1
Magnaporthe oryzae (rice blast fungus)	MGG_08832	XP_003713851.1
Neurospora crassa	NCU06207	XP_962923.1
Arabidopsis thaliana (thale cress)	STE1	NP_186907.1

Gene Ontology
Bibliography

Related articles in PubMed

Defects of cholesterol biosynthesis.
Waterham HR
FEBS letters580(23)5442-9(2006 Oct)

Expression and regulation of delta5-desaturase, delta6-desaturase, stearoyl-coenzyme A (CoA) desaturase 1, and stearoyl-CoA desaturase 2 in rat testis.
Saether T, Tran TN, Rootwelt H, Christophersen BO, Haugen TB
Biology of reproduction69(1)117-24(2003 Jul)

Lathosterolosis: an inborn error of human and murine cholesterol synthesis due to lathosterol 5-desaturase deficiency.
Krakowiak PA, Wassif CA, Kratz L, Cozma D, Kov?rov? M, Harris G, Grinberg A, Yang Y, Hunter AG, Tsokos M, Kelley RI, Porter FD
Human molecular genetics12(13)1631-41(2003 Jul)

Disorders of cholesterol biosynthesis: prototypic metabolic malformation syndromes.
Herman GE
Human molecular genetics12 Spec No 1R75-88(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

testicular distribution of Delta5- and Delta6-desaturase and stearoyl-CoA desaturase 1 and 2 shows that all four desaturases seem to be localized in the Sertoli cells, with far lower expression in germ cells
Title: Expression and regulation of delta5-desaturase, delta6-desaturase, stearoyl-coenzyme A (CoA) desaturase 1, and stearoyl-CoA desaturase 2 in rat testis.

The following Sc5d gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sc5d cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10169	NM_053642.2 Latest version!	Rattus norvegicus sterol-C5-desaturase (Sc5d), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa10169	XM_017595406.1 Latest version!	Rattus norvegicus sterol-C5-desaturase (Sc5d), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sc5d ( NM_053642.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446094.1 Rattus norvegicus sterol-C5-desaturase (Sc5d), mRNA.

Price & Availability↑

CloneID	ORa10169
Clone ID Related Accession (Same CDS sequence)	XM_017595406.1 , NM_053642.2
Accession Version	NM_053642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	lathosterol oxidase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC081704.1. On Sep 16, 2004 this sequence version replaced NM_053642.1. ##Evidence-Data-START## Transcript exon combination :: BC081704.1, AB052846.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACCTGG TTCTCAGTGC TGCCGACTAC TATTTCTTCA CTCCCTATGT GTATCCAGCC 
ACATGGCCCG AGGACAACAT CATCCGACAA ACTGTTAGTC TCCTGGTTGT CACCAACCTG 
GGTGCTTACA TCCTCTACTT CTTCTGCGCG ACCCTTAGCT ATTATTTTGT CTATGACCAT 
TCATTAATGA AACACCCACA GTTTTTAAAG AATCAAGTCT CTCGAGAGAT CATGTTCACT 
GTCAAGTCTT TGCCTTGGAT CAGCATCCCC ACTGTGTCAT TGTTCCTGCT GGAGCTGAGG 
GGTTACAGCA AACTCTATGA TGACATAGGA GACTTTCCAA ATGGCTGGAT TCATCTCATC 
ATGAGCGTGA TCTCCTTCCT CTTCTTCACC GACATGTTGA TCTACTGGAT TCATAGGGGC 
CTGCACCACA GACTGCTCTA TAAGCACATA CATAAACCTC ATCATATTTG GAAGATCCCC 
ACGCCGTTCG CAAGTCATGC TTTTCACCCT GTGGACGGCT TCCTTCAGAG TCTGCCTTAC 
CATATCTACC CCTTTGTCTT CCCGCTGCAC AAGGTGGTCT ACTTAGGTTT ATATGTCTTG 
GTTAATGTCT GGACAATTTC TATTCATGAT GGCGACTTCC GGGTTCCCCA GATCTTCAGG 
CCGTTCATTA ACGGGTCGGC TCATCACACA GACCACCACA TGCTCTTTGA CTATAACTAT 
GGGCAGTACT TCACACTGTG GGATAGAATC GGAGGCTCTT TTAAACATCC TTCCTCTTTC 
GAAGGGAAAG GACCACATAG TTACGTGAAG AACATGACAG AAAAAGAATC CAACAGCCTT 
GCAGAAAATG GCTGCAAAAG CAAAAAATTA TGCAATGGGG AGTTTACAAA GAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446094.1
CDS	125..1024
Translation	MDLVLSAADYYFFTPYVYPATWPEDNIIRQTVSLLVVTNLGAYILYFFCATLSYYFVYDHSLMKHPQFLKNQVSREIMFTVKSLPWISIPTVSLFLLELRGYSKLYDDIGDFPNGWIHLIMSVISFLFFTDMLIYWIHRGLHHRLLYKHIHKPHHIWKIPTPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHDGDFRVPQIFRPFINGSAHHTDHHMLFDYNYGQYFTLWDRIGGSFKHPSSFEGKGPHSYVKNMTEKESNSLAENGCKSKKLCNGEFTKNE

Target ORF information:

RefSeq Version	NM_053642.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sterol-C5-desaturase (Sc5d), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053642.2

ORF Insert Sequence:

ATGGACCTGG TTCTCAGTGC TGCCGACTAC TATTTCTTCA CTCCCTATGT GTATCCAGCC 
ACATGGCCCG AGGACAACAT CATCCGACAA ACTGTTAGTC TCCTGGTTGT CACCAACCTG 
GGTGCTTACA TCCTCTACTT CTTCTGCGCG ACCCTTAGCT ATTATTTTGT CTATGACCAT 
TCATTAATGA AACACCCACA GTTTTTAAAG AATCAAGTCT CTCGAGAGAT CATGTTCACT 
GTCAAGTCTT TGCCTTGGAT CAGCATCCCC ACTGTGTCAT TGTTCCTGCT GGAGCTGAGG 
GGTTACAGCA AACTCTATGA TGACATAGGA GACTTTCCAA ATGGCTGGAT TCATCTCATC 
ATGAGCGTGA TCTCCTTCCT CTTCTTCACC GACATGTTGA TCTACTGGAT TCATAGGGGC 
CTGCACCACA GACTGCTCTA TAAGCACATA CATAAACCTC ATCATATTTG GAAGATCCCC 
ACGCCGTTCG CAAGTCATGC TTTTCACCCT GTGGACGGCT TCCTTCAGAG TCTGCCTTAC 
CATATCTACC CCTTTGTCTT CCCGCTGCAC AAGGTGGTCT ACTTAGGTTT ATATGTCTTG 
GTTAATGTCT GGACAATTTC TATTCATGAT GGCGACTTCC GGGTTCCCCA GATCTTCAGG 
CCGTTCATTA ACGGGTCGGC TCATCACACA GACCACCACA TGCTCTTTGA CTATAACTAT 
GGGCAGTACT TCACACTGTG GGATAGAATC GGAGGCTCTT TTAAACATCC TTCCTCTTTC 
GAAGGGAAAG GACCACATAG TTACGTGAAG AACATGACAG AAAAAGAATC CAACAGCCTT 
GCAGAAAATG GCTGCAAAAG CAAAAAATTA TGCAATGGGG AGTTTACAAA GAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sc5d ( XM_017595406.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450895.1 Rattus norvegicus sterol-C5-desaturase (Sc5d), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10169
Clone ID Related Accession (Same CDS sequence)	XM_017595406.1 , NM_053642.2
Accession Version	XM_017595406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	lathosterol oxidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG TTCTCAGTGC TGCCGACTAC TATTTCTTCA CTCCCTATGT GTATCCAGCC 
ACATGGCCCG AGGACAACAT CATCCGACAA ACTGTTAGTC TCCTGGTTGT CACCAACCTG 
GGTGCTTACA TCCTCTACTT CTTCTGCGCG ACCCTTAGCT ATTATTTTGT CTATGACCAT 
TCATTAATGA AACACCCACA GTTTTTAAAG AATCAAGTCT CTCGAGAGAT CATGTTCACT 
GTCAAGTCTT TGCCTTGGAT CAGCATCCCC ACTGTGTCAT TGTTCCTGCT GGAGCTGAGG 
GGTTACAGCA AACTCTATGA TGACATAGGA GACTTTCCAA ATGGCTGGAT TCATCTCATC 
ATGAGCGTGA TCTCCTTCCT CTTCTTCACC GACATGTTGA TCTACTGGAT TCATAGGGGC 
CTGCACCACA GACTGCTCTA TAAGCACATA CATAAACCTC ATCATATTTG GAAGATCCCC 
ACGCCGTTCG CAAGTCATGC TTTTCACCCT GTGGACGGCT TCCTTCAGAG TCTGCCTTAC 
CATATCTACC CCTTTGTCTT CCCGCTGCAC AAGGTGGTCT ACTTAGGTTT ATATGTCTTG 
GTTAATGTCT GGACAATTTC TATTCATGAT GGCGACTTCC GGGTTCCCCA GATCTTCAGG 
CCGTTCATTA ACGGGTCGGC TCATCACACA GACCACCACA TGCTCTTTGA CTATAACTAT 
GGGCAGTACT TCACACTGTG GGATAGAATC GGAGGCTCTT TTAAACATCC TTCCTCTTTC 
GAAGGGAAAG GACCACATAG TTACGTGAAG AACATGACAG AAAAAGAATC CAACAGCCTT 
GCAGAAAATG GCTGCAAAAG CAAAAAATTA TGCAATGGGG AGTTTACAAA GAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450895.1
CDS	200..1099
Translation	MDLVLSAADYYFFTPYVYPATWPEDNIIRQTVSLLVVTNLGAYILYFFCATLSYYFVYDHSLMKHPQFLKNQVSREIMFTVKSLPWISIPTVSLFLLELRGYSKLYDDIGDFPNGWIHLIMSVISFLFFTDMLIYWIHRGLHHRLLYKHIHKPHHIWKIPTPFASHAFHPVDGFLQSLPYHIYPFVFPLHKVVYLGLYVLVNVWTISIHDGDFRVPQIFRPFINGSAHHTDHHMLFDYNYGQYFTLWDRIGGSFKHPSSFEGKGPHSYVKNMTEKESNSLAENGCKSKKLCNGEFTKNE

Target ORF information:

RefSeq Version	XM_017595406.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sterol-C5-desaturase (Sc5d), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595406.1

ORF Insert Sequence:

ATGGACCTGG TTCTCAGTGC TGCCGACTAC TATTTCTTCA CTCCCTATGT GTATCCAGCC 
ACATGGCCCG AGGACAACAT CATCCGACAA ACTGTTAGTC TCCTGGTTGT CACCAACCTG 
GGTGCTTACA TCCTCTACTT CTTCTGCGCG ACCCTTAGCT ATTATTTTGT CTATGACCAT 
TCATTAATGA AACACCCACA GTTTTTAAAG AATCAAGTCT CTCGAGAGAT CATGTTCACT 
GTCAAGTCTT TGCCTTGGAT CAGCATCCCC ACTGTGTCAT TGTTCCTGCT GGAGCTGAGG 
GGTTACAGCA AACTCTATGA TGACATAGGA GACTTTCCAA ATGGCTGGAT TCATCTCATC 
ATGAGCGTGA TCTCCTTCCT CTTCTTCACC GACATGTTGA TCTACTGGAT TCATAGGGGC 
CTGCACCACA GACTGCTCTA TAAGCACATA CATAAACCTC ATCATATTTG GAAGATCCCC 
ACGCCGTTCG CAAGTCATGC TTTTCACCCT GTGGACGGCT TCCTTCAGAG TCTGCCTTAC 
CATATCTACC CCTTTGTCTT CCCGCTGCAC AAGGTGGTCT ACTTAGGTTT ATATGTCTTG 
GTTAATGTCT GGACAATTTC TATTCATGAT GGCGACTTCC GGGTTCCCCA GATCTTCAGG 
CCGTTCATTA ACGGGTCGGC TCATCACACA GACCACCACA TGCTCTTTGA CTATAACTAT 
GGGCAGTACT TCACACTGTG GGATAGAATC GGAGGCTCTT TTAAACATCC TTCCTCTTTC 
GAAGGGAAAG GACCACATAG TTACGTGAAG AACATGACAG AAAAAGAATC CAACAGCCTT 
GCAGAAAATG GCTGCAAAAG CAAAAAATTA TGCAATGGGG AGTTTACAAA GAACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defects of cholesterol biosynthesis.
FEBS letters580(23)5442-9(2006 Oct)
Waterham HR

Expression and regulation of delta5-desaturase, delta6-desaturase, stearoyl-coenzyme A (CoA) desaturase 1, and stearoyl-CoA desaturase 2 in rat testis.
Biology of reproduction69(1)117-24(2003 Jul)
Saether T,Tran TN,Rootwelt H,Christophersen BO,Haugen TB

Lathosterolosis: an inborn error of human and murine cholesterol synthesis due to lathosterol 5-desaturase deficiency.
Human molecular genetics12(13)1631-41(2003 Jul)
Krakowiak PA,Wassif CA,Kratz L,Cozma D,Kov?rov? M,Harris G,Grinberg A,Yang Y,Hunter AG,Tsokos M,Kelley RI,Porter FD

Disorders of cholesterol biosynthesis: prototypic metabolic malformation syndromes.
Human molecular genetics12 Spec No 1R75-88(2003 Apr)
Herman GE