LOC103465714 gene cDNA ORF clone, Poecilia reticulata(guppy)

mRNA	Protein	Name
XM_008410789.2	XP_008409011.1	pleckstrin homology domain-containing family A member 7-like isoform X3
XM_008410793.2	XP_008409015.1	pleckstrin homology domain-containing family A member 7-like isoform X6
XM_008410799.2	XP_008409021.1	pleckstrin homology domain-containing family A member 7-like isoform X11
XM_017305317.1	XP_017160806.1	pleckstrin homology domain-containing family A member 7-like isoform X12
XM_008410787.2	XP_008409009.1	pleckstrin homology domain-containing family A member 7-like isoform X1
XM_008410794.2	XP_008409016.1	pleckstrin homology domain-containing family A member 7-like isoform X7
XM_017305316.1	XP_017160805.1	pleckstrin homology domain-containing family A member 7-like isoform X8
XM_008410795.2	XP_008409017.1	pleckstrin homology domain-containing family A member 7-like isoform X9
XM_008410804.2	XP_008409026.1	pleckstrin homology domain-containing family A member 7-like isoform X13
XM_017305318.1	XP_017160807.1	pleckstrin homology domain-containing family A member 7-like isoform X14
XM_008410788.2	XP_008409010.1	pleckstrin homology domain-containing family A member 7-like isoform X2
XM_008410791.2	XP_008409013.1	pleckstrin homology domain-containing family A member 7-like isoform X4
XM_008410792.2	XP_008409014.1	pleckstrin homology domain-containing family A member 7-like isoform X5
XM_008410797.2	XP_008409019.1	pleckstrin homology domain-containing family A member 7-like isoform X10
XM_008410801.1	XP_008409023.1	pleckstrin homology domain-containing family A member 7-like isoform X11
XM_008410800.2	XP_008409022.1	pleckstrin homology domain-containing family A member 7-like isoform X11

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPo196440	XM_008410787.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo196441	XM_008410788.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo196442	XM_008410789.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo196443	XM_008410791.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo196444	XM_008410792.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo196445	XM_008410793.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo196446	XM_008410794.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo196447	XM_017305316.1 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OPo196448	XM_008410795.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo196449	XM_008410797.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo196450	XM_008410800.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo196450	XM_008410799.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo196450	XM_008410801.1 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo196451	XM_017305317.1 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OPo196452	XM_008410804.2 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OPo196453	XM_017305318.1 Latest version!	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

LOC103465714 ( XM_008410787.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409009.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPo196440
Clone ID Related Accession (Same CDS sequence)	XM_008410787.2
Accession Version	XM_008410787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410787.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGATCA GAGCTCTAGA GCCGCTGATC CTCGCAGTTC ACAGTTTACT ACTGCACTGC 
ACTGCTGGGC CACTCAGGCC TTTAATGAAC GTAGCTGGAA GTCAGGTGCT GAAGGAGGCG 
GGGAAGCCTG TCAAGGTTGC AGAAAGCGAT GTGGATGTGA AGTTGAGTCG GTTATGTGAG 
CAGGACAAGA TTCTGAAGGA CCTGGAGGCG AGGATCAGCT CCCTGAAGAA CGACAAGGAC 
AAGTTGGAGA GAGTGCTTGA CGTGTCTCAC CAGCAGATGG AGCAGTACCA GGAGCAGCCA 
GCTCATGTCC ACAAGATCGC CTACCAGCAG AGGCTGCTGC AGGAGGACCT GGTGACCATC 
AGGGCCCAGA TTTCCCGCGT CTCAACGGAA ATGGCGCGTG CGTGGGAGGA GTACAACCGG 
CTGGAGCAAT CAGTGGAGCT CCTAAGGCTG GCACTGCAGG CACACATGGC CCACAACGAC 
ACGTCTCAGC AGGAAAAGGC CGAACTGAAG CGGGAGCTGT GGAGGATTGA GGACGTCATG 
GGTGGTCTTA GTGCCAGCAA AGCCAACTAC AAGATAACTG TGGACTCTAT TCAGAATCCA 
GCAGACAGGA GATTAGTGCC TTCGTTGTCG GACCAGGCAT TGCCTTCCGA TGGCGCGGAG 
GTCCAGCCTC CCCCCCGCAG CTCCATCTCC CACACTTTAC CTCACAGCAC CGTGCCAAAG 
TGGGCAGAGG ATGACGCCCC GCCCAGGCCG CCACTGCCTC GTCTCTATGA CTACGAGGAG 
ATGCCCCCCG TTGTGCCCCC GCTCCCCAAG GAGGCCTCGG TCATCCGCCA CACATCGGTG 
CGAGGGCTCA AACGCCAGTC GGACGAGAGG AAGCGGGACA GGGAGGGCGG CCAGTACATT 
GCCAATGGAG ACTGTAAGAC TGATTTGAGG TCATACCTGA GCGAACCAGA GCTGCCAGGA 
AGCAGCCAGC CAATCACAGC ATCTGAAGCT GACTACCAGT ATTACCCAGG CAAAGGTTTG 
TTGGGAACCA CGTCTCGGCT GGACCAGTCC AACTCCATCT CGTCCTACGT CACCTTGAGG 
AGGGCTCCCG GCAGCGCCGC ACCAAAGGAC CGACCCAAAA GCGCCTTTGA GCGCCTGTCA 
TCCCCCACGG AGGGCGGCCC GCCCCCCAGC AGCCAAACCC GAGGCCGGAT GACTGCCGAG 
GAGCAGCTGG AGCGCATGAA GCGCCACCAG AGGGCGCTGG TCCGCGAGCG CAAGAGGAAC 
CTGAGCCACG GCGAGCGCTC TGGCGGCGGG GGCCCGGCAG CATCAGGCTC TCAGCGCTCG 
TCCTCGGCGT CGTCCCGACT GCCCTCCAGC CCCTGCGTGT TCGACTGGCA GCAGGAGCGA 
CCAGGAGCTG AAGGTCAGAG CGACGAAGGC CTGAATCTGG TCAGAGAGAG GGAGACGAGG 
GACTCTAATG AGTGGGTGAC GGTCACCGCC AGGCGCATCC AGGAGGCCGA CCTGGAGCCC 
CTCGACTACG ACCTGGACAT CAGCCGGGAG CTGTCCAAAC CGGACAAAGT TGTGATCCCG 
GAGCGCTACG TGGAGTCGGA CCCAGAGGAG CCTCTGAGTC CAGAGGAGCT GGAGGAACGC 
GGCCGGCGCG CGGAACGCAT CAAGAACCTT CTCACCAGGT CCAGTGTCCA GAACATCCAG 
CCATCAGCAT CGCTGGAATT CAACGAGCTG GACAACGCCA TGGAGGAGCA GGAGAGGATC 
ATGAGCGTCT CCTACGCGCT GGCCTCAGAG GCTTCACGCA AGAGTAAACT GGTCGCAGCC 
AAAGCTGCTG CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409009.1
CDS	170..4330
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410787.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410787.2

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGATCA GAGCTCTAGA GCCGCTGATC CTCGCAGTTC ACAGTTTACT ACTGCACTGC 
ACTGCTGGGC CACTCAGGCC TTTAATGAAC GTAGCTGGAA GTCAGGTGCT GAAGGAGGCG 
GGGAAGCCTG TCAAGGTTGC AGAAAGCGAT GTGGATGTGA AGTTGAGTCG GTTATGTGAG 
CAGGACAAGA TTCTGAAGGA CCTGGAGGCG AGGATCAGCT CCCTGAAGAA CGACAAGGAC 
AAGTTGGAGA GAGTGCTTGA CGTGTCTCAC CAGCAGATGG AGCAGTACCA GGAGCAGCCA 
GCTCATGTCC ACAAGATCGC CTACCAGCAG AGGCTGCTGC AGGAGGACCT GGTGACCATC 
AGGGCCCAGA TTTCCCGCGT CTCAACGGAA ATGGCGCGTG CGTGGGAGGA GTACAACCGG 
CTGGAGCAAT CAGTGGAGCT CCTAAGGCTG GCACTGCAGG CACACATGGC CCACAACGAC 
ACGTCTCAGC AGGAAAAGGC CGAACTGAAG CGGGAGCTGT GGAGGATTGA GGACGTCATG 
GGTGGTCTTA GTGCCAGCAA AGCCAACTAC AAGATAACTG TGGACTCTAT TCAGAATCCA 
GCAGACAGGA GATTAGTGCC TTCGTTGTCG GACCAGGCAT TGCCTTCCGA TGGCGCGGAG 
GTCCAGCCTC CCCCCCGCAG CTCCATCTCC CACACTTTAC CTCACAGCAC CGTGCCAAAG 
TGGGCAGAGG ATGACGCCCC GCCCAGGCCG CCACTGCCTC GTCTCTATGA CTACGAGGAG 
ATGCCCCCCG TTGTGCCCCC GCTCCCCAAG GAGGCCTCGG TCATCCGCCA CACATCGGTG 
CGAGGGCTCA AACGCCAGTC GGACGAGAGG AAGCGGGACA GGGAGGGCGG CCAGTACATT 
GCCAATGGAG ACTGTAAGAC TGATTTGAGG TCATACCTGA GCGAACCAGA GCTGCCAGGA 
AGCAGCCAGC CAATCACAGC ATCTGAAGCT GACTACCAGT ATTACCCAGG CAAAGGTTTG 
TTGGGAACCA CGTCTCGGCT GGACCAGTCC AACTCCATCT CGTCCTACGT CACCTTGAGG 
AGGGCTCCCG GCAGCGCCGC ACCAAAGGAC CGACCCAAAA GCGCCTTTGA GCGCCTGTCA 
TCCCCCACGG AGGGCGGCCC GCCCCCCAGC AGCCAAACCC GAGGCCGGAT GACTGCCGAG 
GAGCAGCTGG AGCGCATGAA GCGCCACCAG AGGGCGCTGG TCCGCGAGCG CAAGAGGAAC 
CTGAGCCACG GCGAGCGCTC TGGCGGCGGG GGCCCGGCAG CATCAGGCTC TCAGCGCTCG 
TCCTCGGCGT CGTCCCGACT GCCCTCCAGC CCCTGCGTGT TCGACTGGCA GCAGGAGCGA 
CCAGGAGCTG AAGGTCAGAG CGACGAAGGC CTGAATCTGG TCAGAGAGAG GGAGACGAGG 
GACTCTAATG AGTGGGTGAC GGTCACCGCC AGGCGCATCC AGGAGGCCGA CCTGGAGCCC 
CTCGACTACG ACCTGGACAT CAGCCGGGAG CTGTCCAAAC CGGACAAAGT TGTGATCCCG 
GAGCGCTACG TGGAGTCGGA CCCAGAGGAG CCTCTGAGTC CAGAGGAGCT GGAGGAACGC 
GGCCGGCGCG CGGAACGCAT CAAGAACCTT CTCACCAGGT CCAGTGTCCA GAACATCCAG 
CCATCAGCAT CGCTGGAATT CAACGAGCTG GACAACGCCA TGGAGGAGCA GGAGAGGATC 
ATGAGCGTCT CCTACGCGCT GGCCTCAGAG GCTTCACGCA AGAGTAAACT GGTCGCAGCC 
AAAGCTGCTG CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410788.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409010.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPo196441
Clone ID Related Accession (Same CDS sequence)	XM_008410788.2
Accession Version	XM_008410788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGATCA GAGCTCTAGA GCCGCTGATC CTCGCAGTTC ACAGTTTACT ACTGCACTGC 
ACTGCTGGGC CACTCAGGCC TTTAATGAAC GTAGCTGGAA GTCAGGTGCT GAAGGAGGCG 
GGGAAGCCTG TCAAGGTTGC AGAAAGCGAT GTGGATGTGA AGTTGAGTCG GTTATGTGAG 
CAGGACAAGA TTCTGAAGGA CCTGGAGGCG AGGATCAGCT CCCTGAAGAA CGACAAGGAC 
AAGTTGGAGA GAGTGCTTGA CGTGTCTCAC CAGCAGATGG AGCAGTACCA GGAGCAGCCA 
GCTCATGTCC ACAAGATCGC CTACCAGCAG AGGCTGCTGC AGGAGGACCT GGTGACCATC 
AGGGCCCAGA TTTCCCGCGT CTCAACGGAA ATGGCGCGTG CGTGGGAGGA GTACAACCGG 
CTGGAGCAAT CAGTGGAGCT CCTAAGGCTG GCACTGCAGG CACACATGGC CCACAACGAC 
ACGTCTCAGC AGGAAAAGGC CGAACTGAAG CGGGAGCTGT GGAGGATTGA GGACGTCATG 
GGTGGTCTTA GTGCCAGCAA AGCCAACTAC AAGATAACTG TGGACTCTAT TCAGAATCCA 
GACAGGAGAT TAGTGCCTTC GTTGTCGGAC CAGGCATTGC CTTCCGATGG CGCGGAGGTC 
CAGCCTCCCC CCCGCAGCTC CATCTCCCAC ACTTTACCTC ACAGCACCGT GCCAAAGTGG 
GCAGAGGATG ACGCCCCGCC CAGGCCGCCA CTGCCTCGTC TCTATGACTA CGAGGAGATG 
CCCCCCGTTG TGCCCCCGCT CCCCAAGGAG GCCTCGGTCA TCCGCCACAC ATCGGTGCGA 
GGGCTCAAAC GCCAGTCGGA CGAGAGGAAG CGGGACAGGG AGGGCGGCCA GTACATTGCC 
AATGGAGACT GTAAGACTGA TTTGAGGTCA TACCTGAGCG AACCAGAGCT GCCAGGAAGC 
AGCCAGCCAA TCACAGCATC TGAAGCTGAC TACCAGTATT ACCCAGGCAA AGGTTTGTTG 
GGAACCACGT CTCGGCTGGA CCAGTCCAAC TCCATCTCGT CCTACGTCAC CTTGAGGAGG 
GCTCCCGGCA GCGCCGCACC AAAGGACCGA CCCAAAAGCG CCTTTGAGCG CCTGTCATCC 
CCCACGGAGG GCGGCCCGCC CCCCAGCAGC CAAACCCGAG GCCGGATGAC TGCCGAGGAG 
CAGCTGGAGC GCATGAAGCG CCACCAGAGG GCGCTGGTCC GCGAGCGCAA GAGGAACCTG 
AGCCACGGCG AGCGCTCTGG CGGCGGGGGC CCGGCAGCAT CAGGCTCTCA GCGCTCGTCC 
TCGGCGTCGT CCCGACTGCC CTCCAGCCCC TGCGTGTTCG ACTGGCAGCA GGAGCGACCA 
GGAGCTGAAG GTCAGAGCGA CGAAGGCCTG AATCTGGTCA GAGAGAGGGA GACGAGGGAC 
TCTAATGAGT GGGTGACGGT CACCGCCAGG CGCATCCAGG AGGCCGACCT GGAGCCCCTC 
GACTACGACC TGGACATCAG CCGGGAGCTG TCCAAACCGG ACAAAGTTGT GATCCCGGAG 
CGCTACGTGG AGTCGGACCC AGAGGAGCCT CTGAGTCCAG AGGAGCTGGA GGAACGCGGC 
CGGCGCGCGG AACGCATCAA GAACCTTCTC ACCAGGTCCA GTGTCCAGAA CATCCAGCCA 
TCAGCATCGC TGGAATTCAA CGAGCTGGAC AACGCCATGG AGGAGCAGGA GAGGATCATG 
AGCGTCTCCT ACGCGCTGGC CTCAGAGGCT TCACGCAAGA GTAAACTGGT CGCAGCCAAA 
GCTGCTGCTC AGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409010.1
CDS	168..4325
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPDRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410788.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410788.2

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGATCA GAGCTCTAGA GCCGCTGATC CTCGCAGTTC ACAGTTTACT ACTGCACTGC 
ACTGCTGGGC CACTCAGGCC TTTAATGAAC GTAGCTGGAA GTCAGGTGCT GAAGGAGGCG 
GGGAAGCCTG TCAAGGTTGC AGAAAGCGAT GTGGATGTGA AGTTGAGTCG GTTATGTGAG 
CAGGACAAGA TTCTGAAGGA CCTGGAGGCG AGGATCAGCT CCCTGAAGAA CGACAAGGAC 
AAGTTGGAGA GAGTGCTTGA CGTGTCTCAC CAGCAGATGG AGCAGTACCA GGAGCAGCCA 
GCTCATGTCC ACAAGATCGC CTACCAGCAG AGGCTGCTGC AGGAGGACCT GGTGACCATC 
AGGGCCCAGA TTTCCCGCGT CTCAACGGAA ATGGCGCGTG CGTGGGAGGA GTACAACCGG 
CTGGAGCAAT CAGTGGAGCT CCTAAGGCTG GCACTGCAGG CACACATGGC CCACAACGAC 
ACGTCTCAGC AGGAAAAGGC CGAACTGAAG CGGGAGCTGT GGAGGATTGA GGACGTCATG 
GGTGGTCTTA GTGCCAGCAA AGCCAACTAC AAGATAACTG TGGACTCTAT TCAGAATCCA 
GACAGGAGAT TAGTGCCTTC GTTGTCGGAC CAGGCATTGC CTTCCGATGG CGCGGAGGTC 
CAGCCTCCCC CCCGCAGCTC CATCTCCCAC ACTTTACCTC ACAGCACCGT GCCAAAGTGG 
GCAGAGGATG ACGCCCCGCC CAGGCCGCCA CTGCCTCGTC TCTATGACTA CGAGGAGATG 
CCCCCCGTTG TGCCCCCGCT CCCCAAGGAG GCCTCGGTCA TCCGCCACAC ATCGGTGCGA 
GGGCTCAAAC GCCAGTCGGA CGAGAGGAAG CGGGACAGGG AGGGCGGCCA GTACATTGCC 
AATGGAGACT GTAAGACTGA TTTGAGGTCA TACCTGAGCG AACCAGAGCT GCCAGGAAGC 
AGCCAGCCAA TCACAGCATC TGAAGCTGAC TACCAGTATT ACCCAGGCAA AGGTTTGTTG 
GGAACCACGT CTCGGCTGGA CCAGTCCAAC TCCATCTCGT CCTACGTCAC CTTGAGGAGG 
GCTCCCGGCA GCGCCGCACC AAAGGACCGA CCCAAAAGCG CCTTTGAGCG CCTGTCATCC 
CCCACGGAGG GCGGCCCGCC CCCCAGCAGC CAAACCCGAG GCCGGATGAC TGCCGAGGAG 
CAGCTGGAGC GCATGAAGCG CCACCAGAGG GCGCTGGTCC GCGAGCGCAA GAGGAACCTG 
AGCCACGGCG AGCGCTCTGG CGGCGGGGGC CCGGCAGCAT CAGGCTCTCA GCGCTCGTCC 
TCGGCGTCGT CCCGACTGCC CTCCAGCCCC TGCGTGTTCG ACTGGCAGCA GGAGCGACCA 
GGAGCTGAAG GTCAGAGCGA CGAAGGCCTG AATCTGGTCA GAGAGAGGGA GACGAGGGAC 
TCTAATGAGT GGGTGACGGT CACCGCCAGG CGCATCCAGG AGGCCGACCT GGAGCCCCTC 
GACTACGACC TGGACATCAG CCGGGAGCTG TCCAAACCGG ACAAAGTTGT GATCCCGGAG 
CGCTACGTGG AGTCGGACCC AGAGGAGCCT CTGAGTCCAG AGGAGCTGGA GGAACGCGGC 
CGGCGCGCGG AACGCATCAA GAACCTTCTC ACCAGGTCCA GTGTCCAGAA CATCCAGCCA 
TCAGCATCGC TGGAATTCAA CGAGCTGGAC AACGCCATGG AGGAGCAGGA GAGGATCATG 
AGCGTCTCCT ACGCGCTGGC CTCAGAGGCT TCACGCAAGA GTAAACTGGT CGCAGCCAAA 
GCTGCTGCTC AGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410789.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409011.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X3, mRNA.

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CloneID	OPo196442
Clone ID Related Accession (Same CDS sequence)	XM_008410789.2
Accession Version	XM_008410789.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410789.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGATCA GAGCTCTAGA GCCGCTGATC CTCGCAGTTC ACAGTTTACT ACTGCACTGC 
ACTGCTGGGC CACTCAGGCC TTTAATGAAC GTAGCTGGAA GTCAGGTGCT GAAGGAGGCG 
GGGAAGCCTG TCAAGGTTGC AGAAAGCGAT GTGGATGTGA AGTTGAGTCG GTTATGTGAG 
CAGGACAAGA TTCTGAAGGA CCTGGAGGCG AGGATCAGCT CCCTGAAGAA CGACAAGGAC 
AAGTTGGAGA GAGTGCTTGA CGTGTCTCAC CAGCAGATGG AGCAGTACCA GGAGCAGCCA 
GCTCATGTCC ACAAGATCGC CTACCAGCAG AGGCTGCTGC AGGAGGACCT GGTGACCATC 
AGGGCCCAGA TTTCCCGCGT CTCAACGGAA ATGGCGCGTG CGTGGGAGGA GTACAACCGG 
CTGGAGCAAT CAGTGGAGCT CCTAAGGCTG GCACTGCAGG CACACATGGC CCACAACGAC 
ACGTCTCAGC AGGAAAAGGC CGAACTGAAG CGGGAGCTGT GGAGGATTGA GGACGTCATG 
GGTGGTCTTA GTGCCAGCAA AGCCAACTAC AAGATAACTG TGGACTCTAT TCAGAATCCA 
GCAGACAGGA GATTAGTGCC TTCGTTGTCG GACCAGGCAT TGCCTTCCGA TGGCGCGGAG 
GTCCAGCCTC CCCCCCGCAG CTCCATCTCC CACACTTTAC CTCACAGCAC CGTGCCAAAG 
TGGGCAGAGG ATGACGCCCC GCCCAGGCCG CCACTGCCTC GTCTCTATGA CTACGAGGAG 
ATGCCCCCCG TTGTGCCCCC GCTCCCCAAG GAGGCCTCGG TCATCCGCCA CACATCGGTG 
CGAGGGCTCA AACGCCAGTC GGACGAGAGG AAGCGGGACA GGGAGGGCGG CCAGTACATT 
GCCAATGGAG ACTGTAAGAC TGATTTGAGG TCATACCTGA GCGAACCAGA GCTGCCAGGA 
AGCAGCCAGC CAATCACAGC ATCTGAAGCT GACTACCAGT ATTACCCAGG CAAAGGTTTG 
TTGGGAACCA CGTCTCGGCT GGACCAGTCC AACTCCATCT CGTCCTACGT CACCTTGAGG 
AGGGCTCCCG GCAGCGCCGC ACCAAAGGAC CGACCCAAAA GCGCCTTTGA GCGCCTGTCA 
TCCCCCACGG AGGGCGGCCC GCCCCCCAGC AGCCAAACCC GAGGCCGGAT GACTGCCGAG 
GAGCAGCTGG AGCGCATGAA GCGCCACCAG AGGGCGCTGG TCCGCGAGCG CAAGAGGAAC 
CTGAGCCACG GCGAGCGCTC TGGCGGCGGG GGCCCGGCAG CATCAGGCTC TCAGCGCTCG 
TCCTCGGCGT CGTCCCGACT GCCCTCCAGC CCCTGCGTGT TCGACTGGCA GCAGGAGCGA 
CCAGGAGCTG AAGGTCAGAG CGACGAAGGC CTGAATCTGG TCAGAGAGAG GGAGACGAGG 
GACTCTAATG AGTGGGTGAC GGTCACCGCC AGGCGCATCC AGGAGGCCGA CCTGGAGCCC 
CTCGACTACG ACCTGGACAT CAGCCGGGAG CTGTCCAAAC CGGACAAAGT TGTGATCCCG 
GAGCGCTACG TGGAGTCGGA CCCAGAGGAG CCTCTGAGTC CAGAGGAGCT GGAGGAACGC 
GGCCGGCGCG CGGAACGCAT CAAGAACCTT CTCACCAGGT CCAGTGTCCA GAACATCCAG 
CCATCAGCAT CGCTGGAATT CAACGAGCTG GACAACGCCA TGGAGGAGCA GGAGAGGATC 
ATGAGCGTCT CCTACGCGCT GGCCTCAGAG GCTTCACGCA AGAGTAAACT GGTCGCAGCC 
AAAGCTGCTG CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409011.1
CDS	170..4270
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410789.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410789.2

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGATCA GAGCTCTAGA GCCGCTGATC CTCGCAGTTC ACAGTTTACT ACTGCACTGC 
ACTGCTGGGC CACTCAGGCC TTTAATGAAC GTAGCTGGAA GTCAGGTGCT GAAGGAGGCG 
GGGAAGCCTG TCAAGGTTGC AGAAAGCGAT GTGGATGTGA AGTTGAGTCG GTTATGTGAG 
CAGGACAAGA TTCTGAAGGA CCTGGAGGCG AGGATCAGCT CCCTGAAGAA CGACAAGGAC 
AAGTTGGAGA GAGTGCTTGA CGTGTCTCAC CAGCAGATGG AGCAGTACCA GGAGCAGCCA 
GCTCATGTCC ACAAGATCGC CTACCAGCAG AGGCTGCTGC AGGAGGACCT GGTGACCATC 
AGGGCCCAGA TTTCCCGCGT CTCAACGGAA ATGGCGCGTG CGTGGGAGGA GTACAACCGG 
CTGGAGCAAT CAGTGGAGCT CCTAAGGCTG GCACTGCAGG CACACATGGC CCACAACGAC 
ACGTCTCAGC AGGAAAAGGC CGAACTGAAG CGGGAGCTGT GGAGGATTGA GGACGTCATG 
GGTGGTCTTA GTGCCAGCAA AGCCAACTAC AAGATAACTG TGGACTCTAT TCAGAATCCA 
GCAGACAGGA GATTAGTGCC TTCGTTGTCG GACCAGGCAT TGCCTTCCGA TGGCGCGGAG 
GTCCAGCCTC CCCCCCGCAG CTCCATCTCC CACACTTTAC CTCACAGCAC CGTGCCAAAG 
TGGGCAGAGG ATGACGCCCC GCCCAGGCCG CCACTGCCTC GTCTCTATGA CTACGAGGAG 
ATGCCCCCCG TTGTGCCCCC GCTCCCCAAG GAGGCCTCGG TCATCCGCCA CACATCGGTG 
CGAGGGCTCA AACGCCAGTC GGACGAGAGG AAGCGGGACA GGGAGGGCGG CCAGTACATT 
GCCAATGGAG ACTGTAAGAC TGATTTGAGG TCATACCTGA GCGAACCAGA GCTGCCAGGA 
AGCAGCCAGC CAATCACAGC ATCTGAAGCT GACTACCAGT ATTACCCAGG CAAAGGTTTG 
TTGGGAACCA CGTCTCGGCT GGACCAGTCC AACTCCATCT CGTCCTACGT CACCTTGAGG 
AGGGCTCCCG GCAGCGCCGC ACCAAAGGAC CGACCCAAAA GCGCCTTTGA GCGCCTGTCA 
TCCCCCACGG AGGGCGGCCC GCCCCCCAGC AGCCAAACCC GAGGCCGGAT GACTGCCGAG 
GAGCAGCTGG AGCGCATGAA GCGCCACCAG AGGGCGCTGG TCCGCGAGCG CAAGAGGAAC 
CTGAGCCACG GCGAGCGCTC TGGCGGCGGG GGCCCGGCAG CATCAGGCTC TCAGCGCTCG 
TCCTCGGCGT CGTCCCGACT GCCCTCCAGC CCCTGCGTGT TCGACTGGCA GCAGGAGCGA 
CCAGGAGCTG AAGGTCAGAG CGACGAAGGC CTGAATCTGG TCAGAGAGAG GGAGACGAGG 
GACTCTAATG AGTGGGTGAC GGTCACCGCC AGGCGCATCC AGGAGGCCGA CCTGGAGCCC 
CTCGACTACG ACCTGGACAT CAGCCGGGAG CTGTCCAAAC CGGACAAAGT TGTGATCCCG 
GAGCGCTACG TGGAGTCGGA CCCAGAGGAG CCTCTGAGTC CAGAGGAGCT GGAGGAACGC 
GGCCGGCGCG CGGAACGCAT CAAGAACCTT CTCACCAGGT CCAGTGTCCA GAACATCCAG 
CCATCAGCAT CGCTGGAATT CAACGAGCTG GACAACGCCA TGGAGGAGCA GGAGAGGATC 
ATGAGCGTCT CCTACGCGCT GGCCTCAGAG GCTTCACGCA AGAGTAAACT GGTCGCAGCC 
AAAGCTGCTG CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410791.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409013.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X4, mRNA.

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CloneID	OPo196443
Clone ID Related Accession (Same CDS sequence)	XM_008410791.2
Accession Version	XM_008410791.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410791.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTACCT GCTGCAGCCA TTTGCCCTCA CTGGAGGCTG GTCACGAGCC AGTCGCCATC 
AAACGTCACG GCGTCTGGTC AAAAGCTCCT ACAGCGGTCA CTAACCTTAG TGACCGTGAG 
TTCTCCACCT GGGATCTCAT ATGTGACGCA AGTCATAACC AAAAGAGCAC GTCGTTCTGT 
CATCCTGTAA CTGGACAGAT TTCCTCAGAG AACGTGGACT TCCTCCTGCA GGAACAGTCG 
CAAGGTCCTC GCATGATGTC CAGATTCACG GCTGAGCAGC TCTCCAGCAC CACTGTGAGC 
GAGGCCTCCA CCGCCATCAC CTCCTCCACC GTGGACTCCA CCTCTGCCTC CAAGGCATCT 
CGATCCAGTG GGAAGACTCA CAACTTTGGG AAAAGAGAAC AATCCATCAA GAGGAATCCT 
AACGTCCCAG TGGTGGTCCG AGGATGGCTG TACAAACAGG ACAGCTCTGG GATGCGCCTT 
TGGAAGAGGA AGTGGTTTGT CTTGTCTGAT TTCTGCCTTT TCTACTACAA AGACAGCAGA 
GAGGAGGCGG TCTTGGGCAG TATTCCTCTG CCCAGCTATG TCATTTCACC CGTTGGCCCC 
GAAGATCACA TCAGCCGCAA GTATGCCTTC AAGGCTAGCC ACACCGGTAT GCGTTCATAC 
ATTTACAAGC AGAGCTCTGT GATTGGCTCG CAGGCAGAGC ACACTGGCAT GCGGACGTAT 
TACTTCAGCG CTGACACCCA GGAGGACATG AGCACCTGGC TGAAGGCCAT GAACCAGGCA 
TCGCTGATGC AGAACCACCC CGACAGGCTC AGACCCGTTG ACCAAGTCAC CTCGTTGTTG 
CAACAAGCTG TCCCGCAGAC AAACCATGTG AATCACCACA AGATGAAGAC CGTAGACTCT 
GAAACCACAA AAGCAATTGT CCACGAAGTT CTGCTGGAGC CCATTCACCG TAACACAGAA 
GAGCTCTGCA GCTTTCACCA GGACGCCGGC AACTTCACCC TGGAGCGCCA CGCGGGGACC 
GCCTCTTTGG AGATGGACAA ACAGAGCCCC CTTCCATCCA GACCGCCAGC CGCCTCCACA 
CTCCCTCTGT CGGACCGCGC ATCCGCCTCT GCTCCCGTGT CCAGGGTGAC GTCCCGCGCT 
CCGTCCCGCT CCGCCTCGAC ATTGCCCTCC AGCCTTTGCA TGAGGAACGG CCTGGTGTCC 
ACGCCCAGCC CCATCCTGGA GCCCAACGGC ATCGCGGCGG GGACGTACCA GAGGGCGCCG 
GAGCCGGCAG CCTCCGACAC GTTCAAGCAT CTGCAGAGGA GGAACACTCT GGAGCAGGTG 
GAGCAGTGGG TCAAAGTTCA GAAGGCTGAG CAAAGGGGCC CGCCCTCCAG AGAAAACACC 
CTTCCCCGCC GGACGCCGCC CACGCAGAAC AAGTACACCT CCATGGATGC CTTTCAAACT 
TTGCCAAAAA CTCCACGCCA CAGCCCCCCA TCGGGTCGAC TCGGCGAGTA CAAGTACGCC 
CACGACCGCC TGAGCCACTT CCGCCTCACC CCAGACCAGG GGGGCACGGG GTCCAACACA 
GTGCTGCAGC TGTACGAGTG GCAGCAGCGC CAACAGTTCC GTCATGGCAG CCCCACGGCG 
CCAATCTACA CCCCGGCCCC GGATTACCCG TTCGGGCCCC GCCCCCCGTC CACCGTGCCG 
CCTTCCTCCT TGTTGCGCAG GTCGGAGGGG TCTCCTCGCT GCGTGTCGGT GCCACCGTCG 
GCCGCAGACA TCCCGCCACC GGGGCCTCCG CCAGGCACCA GCAGGACGCT GTCCCCCAAC 
CGAAGGCCGC ACACTCCTGC CGAACGGGTG ACTGTAAGGC CCGCTGGGGA CGGGCCCCAG 
CTGAACGTCC CCTTCACCGT GTCCCCCCGC AGGAGCAAGT CTCAGATGCT GAAGGCTGCT 
ACTATTGAGA GACGCTCGAT GCCTTCAACT GGCTACATCA CTCACACAGT CAGCGCTCCC 
AGCCTTCATG GCAAGACGCC GGAGGAGCTC ACTCTGCTCC TCATTCAACT GCGCCGCCAC 
CAGGCCAAGA TGGCCGCTGT GCGTCAGCAG ACCTTAGCTC AGATCCAGCG GCTGAACGCT 
CCCAGAGAGT CCTGCCACCA AAACCGCCTC CTCACCGCTG TCGCCGTTAG CGACAGCCCC 
CTCCTCCTCA GCGCCACCGC GTCTCCTGAG TCGCACCTCG AAGATGTCGG CCTCTGCAGC 
GTCCAGGCTG ATGACACGTA CATGCAGCTG AAGAAGGACC TGGAATATCT GGACCTAAAG 
ATCAGAGCTC TAGAGCCGCT GATCCTCGCA GTTCACAGTT TACTACTGCA CTGCACTGCT 
GGGCCACTCA GGCCTTTAAT GAACGTAGCT GGAAGTCAGG TGCTGAAGGA GGCGGGGAAG 
CCTGTCAAGG TTGCAGAAAG CGATGTGGAT GTGAAGTTGA GTCGGTTATG TGAGCAGGAC 
AAGATTCTGA AGGACCTGGA GGCGAGGATC AGCTCCCTGA AGAACGACAA GGACAAGTTG 
GAGAGAGTGC TTGACGTGTC TCACCAGCAG ATGGAGCAGT ACCAGGAGCA GCCAGCTCAT 
GTCCACAAGA TCGCCTACCA GCAGAGGCTG CTGCAGGAGG ACCTGGTGAC CATCAGGGCC 
CAGATTTCCC GCGTCTCAAC GGAAATGGCG CGTGCGTGGG AGGAGTACAA CCGGCTGGAG 
CAATCAGTGG AGCTCCTAAG GCTGGCACTG CAGGCACACA TGGCCCACAA CGACACGTCT 
CAGCAGGAAA AGGCCGAACT GAAGCGGGAG CTGTGGAGGA TTGAGGACGT CATGGGTGGT 
CTTAGTGCCA GCAAAGCCAA CTACAAGATA ACTGTGGACT CTATTCAGAA TCCAGCAGAC 
AGGAGATTAG TGCCTTCGTT GTCGGACCAG GCATTGCCTT CCGATGGCGC GGAGGTCCAG 
CCTCCCCCCC GCAGCTCCAT CTCCCACACT TTACCTCACA GCACCGTGCC AAAGTGGGCA 
GAGGATGACG CCCCGCCCAG GCCGCCACTG CCTCGTCTCT ATGACTACGA GGAGATGCCC 
CCCGTTGTGC CCCCGCTCCC CAAGGAGGCC TCGGTCATCC GCCACACATC GGTGCGAGGG 
CTCAAACGCC AGTCGGACGA GAGGAAGCGG GACAGGGAGG GCGGCCAGTA CATTGCCAAT 
GGAGACTGTA AGACTGATTT GAGGTCATAC CTGAGCGAAC CAGAGCTGCC AGGAAGCAGC 
CAGCCAATCA CAGCATCTGA AGCTGACTAC CAGTATTACC CAGGCAAAGG TTTGTTGGGA 
ACCACGTCTC GGCTGGACCA GTCCAACTCC ATCTCGTCCT ACGTCACCTT GAGGAGGGCT 
CCCGGCAGCG CCGCACCAAA GGACCGACCC AAAAGCGCCT TTGAGCGCCT GTCATCCCCC 
ACGGAGGGCG GCCCGCCCCC CAGCAGCCAA ACCCGAGGCC GGATGACTGC CGAGGAGCAG 
CTGGAGCGCA TGAAGCGCCA CCAGAGGGCG CTGGTCCGCG AGCGCAAGAG GAACCTGAGC 
CACGGCGAGC GCTCTGGCGG CGGGGGCCCG GCAGCATCAG GCTCTCAGCG CTCGTCCTCG 
GCGTCGTCCC GACTGCCCTC CAGCCCCTGC GTGTTCGACT GGCAGCAGGA GCGACCAGGA 
GCTGAAGGTC AGAGCGACGA AGGCCTGAAT CTGGTCAGAG AGAGGGAGAC GAGGGACTCT 
AATGAGTGGG TGACGGTCAC CGCCAGGCGC ATCCAGGAGG CCGACCTGGA GCCCCTCGAC 
TACGACCTGG ACATCAGCCG GGAGCTGTCC AAACCGGACA AAGTTGTGAT CCCGGAGCGC 
TACGTGGAGT CGGACCCAGA GGAGCCTCTG AGTCCAGAGG AGCTGGAGGA ACGCGGCCGG 
CGCGCGGAAC GCATCAAGAA CCTTCTCACC AGGTCCAGTG TCCAGAACAT CCAGCCATCA 
GCATCGCTGG AATTCAACGA GCTGGACAAC GCCATGGAGG AGCAGGAGAG GATCATGAGC 
GTCTCCTACG CGCTGGCCTC AGAGGCTTCA CGCAAGAGTA AACTGGTCGC AGCCAAAGCT 
GCTGCTCAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409013.1
CDS	180..4274
Translation	MGTCCSHLPSLEAGHEPVAIKRHGVWSKAPTAVTNLSDREFSTWDLICDASHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410791.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410791.2

ORF Insert Sequence:

ATGGGTACCT GCTGCAGCCA TTTGCCCTCA CTGGAGGCTG GTCACGAGCC AGTCGCCATC 
AAACGTCACG GCGTCTGGTC AAAAGCTCCT ACAGCGGTCA CTAACCTTAG TGACCGTGAG 
TTCTCCACCT GGGATCTCAT ATGTGACGCA AGTCATAACC AAAAGAGCAC GTCGTTCTGT 
CATCCTGTAA CTGGACAGAT TTCCTCAGAG AACGTGGACT TCCTCCTGCA GGAACAGTCG 
CAAGGTCCTC GCATGATGTC CAGATTCACG GCTGAGCAGC TCTCCAGCAC CACTGTGAGC 
GAGGCCTCCA CCGCCATCAC CTCCTCCACC GTGGACTCCA CCTCTGCCTC CAAGGCATCT 
CGATCCAGTG GGAAGACTCA CAACTTTGGG AAAAGAGAAC AATCCATCAA GAGGAATCCT 
AACGTCCCAG TGGTGGTCCG AGGATGGCTG TACAAACAGG ACAGCTCTGG GATGCGCCTT 
TGGAAGAGGA AGTGGTTTGT CTTGTCTGAT TTCTGCCTTT TCTACTACAA AGACAGCAGA 
GAGGAGGCGG TCTTGGGCAG TATTCCTCTG CCCAGCTATG TCATTTCACC CGTTGGCCCC 
GAAGATCACA TCAGCCGCAA GTATGCCTTC AAGGCTAGCC ACACCGGTAT GCGTTCATAC 
ATTTACAAGC AGAGCTCTGT GATTGGCTCG CAGGCAGAGC ACACTGGCAT GCGGACGTAT 
TACTTCAGCG CTGACACCCA GGAGGACATG AGCACCTGGC TGAAGGCCAT GAACCAGGCA 
TCGCTGATGC AGAACCACCC CGACAGGCTC AGACCCGTTG ACCAAGTCAC CTCGTTGTTG 
CAACAAGCTG TCCCGCAGAC AAACCATGTG AATCACCACA AGATGAAGAC CGTAGACTCT 
GAAACCACAA AAGCAATTGT CCACGAAGTT CTGCTGGAGC CCATTCACCG TAACACAGAA 
GAGCTCTGCA GCTTTCACCA GGACGCCGGC AACTTCACCC TGGAGCGCCA CGCGGGGACC 
GCCTCTTTGG AGATGGACAA ACAGAGCCCC CTTCCATCCA GACCGCCAGC CGCCTCCACA 
CTCCCTCTGT CGGACCGCGC ATCCGCCTCT GCTCCCGTGT CCAGGGTGAC GTCCCGCGCT 
CCGTCCCGCT CCGCCTCGAC ATTGCCCTCC AGCCTTTGCA TGAGGAACGG CCTGGTGTCC 
ACGCCCAGCC CCATCCTGGA GCCCAACGGC ATCGCGGCGG GGACGTACCA GAGGGCGCCG 
GAGCCGGCAG CCTCCGACAC GTTCAAGCAT CTGCAGAGGA GGAACACTCT GGAGCAGGTG 
GAGCAGTGGG TCAAAGTTCA GAAGGCTGAG CAAAGGGGCC CGCCCTCCAG AGAAAACACC 
CTTCCCCGCC GGACGCCGCC CACGCAGAAC AAGTACACCT CCATGGATGC CTTTCAAACT 
TTGCCAAAAA CTCCACGCCA CAGCCCCCCA TCGGGTCGAC TCGGCGAGTA CAAGTACGCC 
CACGACCGCC TGAGCCACTT CCGCCTCACC CCAGACCAGG GGGGCACGGG GTCCAACACA 
GTGCTGCAGC TGTACGAGTG GCAGCAGCGC CAACAGTTCC GTCATGGCAG CCCCACGGCG 
CCAATCTACA CCCCGGCCCC GGATTACCCG TTCGGGCCCC GCCCCCCGTC CACCGTGCCG 
CCTTCCTCCT TGTTGCGCAG GTCGGAGGGG TCTCCTCGCT GCGTGTCGGT GCCACCGTCG 
GCCGCAGACA TCCCGCCACC GGGGCCTCCG CCAGGCACCA GCAGGACGCT GTCCCCCAAC 
CGAAGGCCGC ACACTCCTGC CGAACGGGTG ACTGTAAGGC CCGCTGGGGA CGGGCCCCAG 
CTGAACGTCC CCTTCACCGT GTCCCCCCGC AGGAGCAAGT CTCAGATGCT GAAGGCTGCT 
ACTATTGAGA GACGCTCGAT GCCTTCAACT GGCTACATCA CTCACACAGT CAGCGCTCCC 
AGCCTTCATG GCAAGACGCC GGAGGAGCTC ACTCTGCTCC TCATTCAACT GCGCCGCCAC 
CAGGCCAAGA TGGCCGCTGT GCGTCAGCAG ACCTTAGCTC AGATCCAGCG GCTGAACGCT 
CCCAGAGAGT CCTGCCACCA AAACCGCCTC CTCACCGCTG TCGCCGTTAG CGACAGCCCC 
CTCCTCCTCA GCGCCACCGC GTCTCCTGAG TCGCACCTCG AAGATGTCGG CCTCTGCAGC 
GTCCAGGCTG ATGACACGTA CATGCAGCTG AAGAAGGACC TGGAATATCT GGACCTAAAG 
ATCAGAGCTC TAGAGCCGCT GATCCTCGCA GTTCACAGTT TACTACTGCA CTGCACTGCT 
GGGCCACTCA GGCCTTTAAT GAACGTAGCT GGAAGTCAGG TGCTGAAGGA GGCGGGGAAG 
CCTGTCAAGG TTGCAGAAAG CGATGTGGAT GTGAAGTTGA GTCGGTTATG TGAGCAGGAC 
AAGATTCTGA AGGACCTGGA GGCGAGGATC AGCTCCCTGA AGAACGACAA GGACAAGTTG 
GAGAGAGTGC TTGACGTGTC TCACCAGCAG ATGGAGCAGT ACCAGGAGCA GCCAGCTCAT 
GTCCACAAGA TCGCCTACCA GCAGAGGCTG CTGCAGGAGG ACCTGGTGAC CATCAGGGCC 
CAGATTTCCC GCGTCTCAAC GGAAATGGCG CGTGCGTGGG AGGAGTACAA CCGGCTGGAG 
CAATCAGTGG AGCTCCTAAG GCTGGCACTG CAGGCACACA TGGCCCACAA CGACACGTCT 
CAGCAGGAAA AGGCCGAACT GAAGCGGGAG CTGTGGAGGA TTGAGGACGT CATGGGTGGT 
CTTAGTGCCA GCAAAGCCAA CTACAAGATA ACTGTGGACT CTATTCAGAA TCCAGCAGAC 
AGGAGATTAG TGCCTTCGTT GTCGGACCAG GCATTGCCTT CCGATGGCGC GGAGGTCCAG 
CCTCCCCCCC GCAGCTCCAT CTCCCACACT TTACCTCACA GCACCGTGCC AAAGTGGGCA 
GAGGATGACG CCCCGCCCAG GCCGCCACTG CCTCGTCTCT ATGACTACGA GGAGATGCCC 
CCCGTTGTGC CCCCGCTCCC CAAGGAGGCC TCGGTCATCC GCCACACATC GGTGCGAGGG 
CTCAAACGCC AGTCGGACGA GAGGAAGCGG GACAGGGAGG GCGGCCAGTA CATTGCCAAT 
GGAGACTGTA AGACTGATTT GAGGTCATAC CTGAGCGAAC CAGAGCTGCC AGGAAGCAGC 
CAGCCAATCA CAGCATCTGA AGCTGACTAC CAGTATTACC CAGGCAAAGG TTTGTTGGGA 
ACCACGTCTC GGCTGGACCA GTCCAACTCC ATCTCGTCCT ACGTCACCTT GAGGAGGGCT 
CCCGGCAGCG CCGCACCAAA GGACCGACCC AAAAGCGCCT TTGAGCGCCT GTCATCCCCC 
ACGGAGGGCG GCCCGCCCCC CAGCAGCCAA ACCCGAGGCC GGATGACTGC CGAGGAGCAG 
CTGGAGCGCA TGAAGCGCCA CCAGAGGGCG CTGGTCCGCG AGCGCAAGAG GAACCTGAGC 
CACGGCGAGC GCTCTGGCGG CGGGGGCCCG GCAGCATCAG GCTCTCAGCG CTCGTCCTCG 
GCGTCGTCCC GACTGCCCTC CAGCCCCTGC GTGTTCGACT GGCAGCAGGA GCGACCAGGA 
GCTGAAGGTC AGAGCGACGA AGGCCTGAAT CTGGTCAGAG AGAGGGAGAC GAGGGACTCT 
AATGAGTGGG TGACGGTCAC CGCCAGGCGC ATCCAGGAGG CCGACCTGGA GCCCCTCGAC 
TACGACCTGG ACATCAGCCG GGAGCTGTCC AAACCGGACA AAGTTGTGAT CCCGGAGCGC 
TACGTGGAGT CGGACCCAGA GGAGCCTCTG AGTCCAGAGG AGCTGGAGGA ACGCGGCCGG 
CGCGCGGAAC GCATCAAGAA CCTTCTCACC AGGTCCAGTG TCCAGAACAT CCAGCCATCA 
GCATCGCTGG AATTCAACGA GCTGGACAAC GCCATGGAGG AGCAGGAGAG GATCATGAGC 
GTCTCCTACG CGCTGGCCTC AGAGGCTTCA CGCAAGAGTA AACTGGTCGC AGCCAAAGCT 
GCTGCTCAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410792.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409014.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OPo196444
Clone ID Related Accession (Same CDS sequence)	XM_008410792.2
Accession Version	XM_008410792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGGTAG CTGGAAGTCA GGTGCTGAAG GAGGCGGGGA AGCCTGTCAA GGTTGCAGAA 
AGCGATGTGG ATGTGAAGTT GAGTCGGTTA TGTGAGCAGG ACAAGATTCT GAAGGACCTG 
GAGGCGAGGA TCAGCTCCCT GAAGAACGAC AAGGACAAGT TGGAGAGAGT GCTTGACGTG 
TCTCACCAGC AGATGGAGCA GTACCAGGAG CAGCCAGCTC ATGTCCACAA GATCGCCTAC 
CAGCAGAGGC TGCTGCAGGA GGACCTGGTG ACCATCAGGG CCCAGATTTC CCGCGTCTCA 
ACGGAAATGG CGCGTGCGTG GGAGGAGTAC AACCGGCTGG AGCAATCAGT GGAGCTCCTA 
AGGCTGGCAC TGCAGGCACA CATGGCCCAC AACGACACGT CTCAGCAGGA AAAGGCCGAA 
CTGAAGCGGG AGCTGTGGAG GATTGAGGAC GTCATGGGTG GTCTTAGTGC CAGCAAAGCC 
AACTACAAGA TAACTGTGGA CTCTATTCAG AATCCAGCAG ACAGGAGATT AGTGCCTTCG 
TTGTCGGACC AGGCATTGCC TTCCGATGGC GCGGAGGTCC AGCCTCCCCC CCGCAGCTCC 
ATCTCCCACA CTTTACCTCA CAGCACCGTG CCAAAGTGGG CAGAGGATGA CGCCCCGCCC 
AGGCCGCCAC TGCCTCGTCT CTATGACTAC GAGGAGATGC CCCCCGTTGT GCCCCCGCTC 
CCCAAGGAGG CCTCGGTCAT CCGCCACACA TCGGTGCGAG GGCTCAAACG CCAGTCGGAC 
GAGAGGAAGC GGGACAGGGA GGGCGGCCAG TACATTGCCA ATGGAGACTG TAAGACTGAT 
TTGAGGTCAT ACCTGAGCGA ACCAGAGCTG CCAGGAAGCA GCCAGCCAAT CACAGCATCT 
GAAGCTGACT ACCAGTATTA CCCAGGCAAA GGTTTGTTGG GAACCACGTC TCGGCTGGAC 
CAGTCCAACT CCATCTCGTC CTACGTCACC TTGAGGAGGG CTCCCGGCAG CGCCGCACCA 
AAGGACCGAC CCAAAAGCGC CTTTGAGCGC CTGTCATCCC CCACGGAGGG CGGCCCGCCC 
CCCAGCAGCC AAACCCGAGG CCGGATGACT GCCGAGGAGC AGCTGGAGCG CATGAAGCGC 
CACCAGAGGG CGCTGGTCCG CGAGCGCAAG AGGAACCTGA GCCACGGCGA GCGCTCTGGC 
GGCGGGGGCC CGGCAGCATC AGGCTCTCAG CGCTCGTCCT CGGCGTCGTC CCGACTGCCC 
TCCAGCCCCT GCGTGTTCGA CTGGCAGCAG GAGCGACCAG GAGCTGAAGG TCAGAGCGAC 
GAAGGCCTGA ATCTGGTCAG AGAGAGGGAG ACGAGGGACT CTAATGAGTG GGTGACGGTC 
ACCGCCAGGC GCATCCAGGA GGCCGACCTG GAGCCCCTCG ACTACGACCT GGACATCAGC 
CGGGAGCTGT CCAAACCGGA CAAAGTTGTG ATCCCGGAGC GCTACGTGGA GTCGGACCCA 
GAGGAGCCTC TGAGTCCAGA GGAGCTGGAG GAACGCGGCC GGCGCGCGGA ACGCATCAAG 
AACCTTCTCA CCAGGTCCAG TGTCCAGAAC ATCCAGCCAT CAGCATCGCT GGAATTCAAC 
GAGCTGGACA ACGCCATGGA GGAGCAGGAG AGGATCATGA GCGTCTCCTA CGCGCTGGCC 
TCAGAGGCTT CACGCAAGAG TAAACTGGTC GCAGCCAAAG CTGCTGCTCA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409014.1
CDS	168..4244
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410792.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410792.2

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGGTAG CTGGAAGTCA GGTGCTGAAG GAGGCGGGGA AGCCTGTCAA GGTTGCAGAA 
AGCGATGTGG ATGTGAAGTT GAGTCGGTTA TGTGAGCAGG ACAAGATTCT GAAGGACCTG 
GAGGCGAGGA TCAGCTCCCT GAAGAACGAC AAGGACAAGT TGGAGAGAGT GCTTGACGTG 
TCTCACCAGC AGATGGAGCA GTACCAGGAG CAGCCAGCTC ATGTCCACAA GATCGCCTAC 
CAGCAGAGGC TGCTGCAGGA GGACCTGGTG ACCATCAGGG CCCAGATTTC CCGCGTCTCA 
ACGGAAATGG CGCGTGCGTG GGAGGAGTAC AACCGGCTGG AGCAATCAGT GGAGCTCCTA 
AGGCTGGCAC TGCAGGCACA CATGGCCCAC AACGACACGT CTCAGCAGGA AAAGGCCGAA 
CTGAAGCGGG AGCTGTGGAG GATTGAGGAC GTCATGGGTG GTCTTAGTGC CAGCAAAGCC 
AACTACAAGA TAACTGTGGA CTCTATTCAG AATCCAGCAG ACAGGAGATT AGTGCCTTCG 
TTGTCGGACC AGGCATTGCC TTCCGATGGC GCGGAGGTCC AGCCTCCCCC CCGCAGCTCC 
ATCTCCCACA CTTTACCTCA CAGCACCGTG CCAAAGTGGG CAGAGGATGA CGCCCCGCCC 
AGGCCGCCAC TGCCTCGTCT CTATGACTAC GAGGAGATGC CCCCCGTTGT GCCCCCGCTC 
CCCAAGGAGG CCTCGGTCAT CCGCCACACA TCGGTGCGAG GGCTCAAACG CCAGTCGGAC 
GAGAGGAAGC GGGACAGGGA GGGCGGCCAG TACATTGCCA ATGGAGACTG TAAGACTGAT 
TTGAGGTCAT ACCTGAGCGA ACCAGAGCTG CCAGGAAGCA GCCAGCCAAT CACAGCATCT 
GAAGCTGACT ACCAGTATTA CCCAGGCAAA GGTTTGTTGG GAACCACGTC TCGGCTGGAC 
CAGTCCAACT CCATCTCGTC CTACGTCACC TTGAGGAGGG CTCCCGGCAG CGCCGCACCA 
AAGGACCGAC CCAAAAGCGC CTTTGAGCGC CTGTCATCCC CCACGGAGGG CGGCCCGCCC 
CCCAGCAGCC AAACCCGAGG CCGGATGACT GCCGAGGAGC AGCTGGAGCG CATGAAGCGC 
CACCAGAGGG CGCTGGTCCG CGAGCGCAAG AGGAACCTGA GCCACGGCGA GCGCTCTGGC 
GGCGGGGGCC CGGCAGCATC AGGCTCTCAG CGCTCGTCCT CGGCGTCGTC CCGACTGCCC 
TCCAGCCCCT GCGTGTTCGA CTGGCAGCAG GAGCGACCAG GAGCTGAAGG TCAGAGCGAC 
GAAGGCCTGA ATCTGGTCAG AGAGAGGGAG ACGAGGGACT CTAATGAGTG GGTGACGGTC 
ACCGCCAGGC GCATCCAGGA GGCCGACCTG GAGCCCCTCG ACTACGACCT GGACATCAGC 
CGGGAGCTGT CCAAACCGGA CAAAGTTGTG ATCCCGGAGC GCTACGTGGA GTCGGACCCA 
GAGGAGCCTC TGAGTCCAGA GGAGCTGGAG GAACGCGGCC GGCGCGCGGA ACGCATCAAG 
AACCTTCTCA CCAGGTCCAG TGTCCAGAAC ATCCAGCCAT CAGCATCGCT GGAATTCAAC 
GAGCTGGACA ACGCCATGGA GGAGCAGGAG AGGATCATGA GCGTCTCCTA CGCGCTGGCC 
TCAGAGGCTT CACGCAAGAG TAAACTGGTC GCAGCCAAAG CTGCTGCTCA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410793.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409015.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OPo196445
Clone ID Related Accession (Same CDS sequence)	XM_008410793.2
Accession Version	XM_008410793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGGTAG CTGGAAGTCA GGTGCTGAAG GAGGCGGGGA AGCCTGTCAA GGTTGCAGAA 
AGCGATGTGG ATGTGAAGTT GAGTCGGTTA TGTGAGCAGG ACAAGATTCT GAAGGACCTG 
GAGGCGAGGA TCAGCTCCCT GAAGAACGAC AAGGACAAGT TGGAGAGAGT GCTTGACGTG 
TCTCACCAGC AGATGGAGCA GTACCAGGAG CAGCCAGCTC ATGTCCACAA GATCGCCTAC 
CAGCAGAGGC TGCTGCAGGA GGACCTGGTG ACCATCAGGG CCCAGATTTC CCGCGTCTCA 
ACGGAAATGG CGCGTGCGTG GGAGGAGTAC AACCGGCTGG AGCAATCAGT GGAGCTCCTA 
AGGCTGGCAC TGCAGGCACA CATGGCCCAC AACGACACGT CTCAGCAGGA AAAGGCCGAA 
CTGAAGCGGG AGCTGTGGAG GATTGAGGAC GTCATGGGTG GTCTTAGTGC CAGCAAAGCC 
AACTACAAGA TAACTGTGGA CTCTATTCAG AATCCAGACA GGAGATTAGT GCCTTCGTTG 
TCGGACCAGG CATTGCCTTC CGATGGCGCG GAGGTCCAGC CTCCCCCCCG CAGCTCCATC 
TCCCACACTT TACCTCACAG CACCGTGCCA AAGTGGGCAG AGGATGACGC CCCGCCCAGG 
CCGCCACTGC CTCGTCTCTA TGACTACGAG GAGATGCCCC CCGTTGTGCC CCCGCTCCCC 
AAGGAGGCCT CGGTCATCCG CCACACATCG GTGCGAGGGC TCAAACGCCA GTCGGACGAG 
AGGAAGCGGG ACAGGGAGGG CGGCCAGTAC ATTGCCAATG GAGACTGTAA GACTGATTTG 
AGGTCATACC TGAGCGAACC AGAGCTGCCA GGAAGCAGCC AGCCAATCAC AGCATCTGAA 
GCTGACTACC AGTATTACCC AGGCAAAGGT TTGTTGGGAA CCACGTCTCG GCTGGACCAG 
TCCAACTCCA TCTCGTCCTA CGTCACCTTG AGGAGGGCTC CCGGCAGCGC CGCACCAAAG 
GACCGACCCA AAAGCGCCTT TGAGCGCCTG TCATCCCCCA CGGAGGGCGG CCCGCCCCCC 
AGCAGCCAAA CCCGAGGCCG GATGACTGCC GAGGAGCAGC TGGAGCGCAT GAAGCGCCAC 
CAGAGGGCGC TGGTCCGCGA GCGCAAGAGG AACCTGAGCC ACGGCGAGCG CTCTGGCGGC 
GGGGGCCCGG CAGCATCAGG CTCTCAGCGC TCGTCCTCGG CGTCGTCCCG ACTGCCCTCC 
AGCCCCTGCG TGTTCGACTG GCAGCAGGAG CGACCAGGAG CTGAAGGTCA GAGCGACGAA 
GGCCTGAATC TGGTCAGAGA GAGGGAGACG AGGGACTCTA ATGAGTGGGT GACGGTCACC 
GCCAGGCGCA TCCAGGAGGC CGACCTGGAG CCCCTCGACT ACGACCTGGA CATCAGCCGG 
GAGCTGTCCA AACCGGACAA AGTTGTGATC CCGGAGCGCT ACGTGGAGTC GGACCCAGAG 
GAGCCTCTGA GTCCAGAGGA GCTGGAGGAA CGCGGCCGGC GCGCGGAACG CATCAAGAAC 
CTTCTCACCA GGTCCAGTGT CCAGAACATC CAGCCATCAG CATCGCTGGA ATTCAACGAG 
CTGGACAACG CCATGGAGGA GCAGGAGAGG ATCATGAGCG TCTCCTACGC GCTGGCCTCA 
GAGGCTTCAC GCAAGAGTAA ACTGGTCGCA GCCAAAGCTG CTGCTCAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409015.1
CDS	167..4240
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPDRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410793.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410793.2

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGGTAG CTGGAAGTCA GGTGCTGAAG GAGGCGGGGA AGCCTGTCAA GGTTGCAGAA 
AGCGATGTGG ATGTGAAGTT GAGTCGGTTA TGTGAGCAGG ACAAGATTCT GAAGGACCTG 
GAGGCGAGGA TCAGCTCCCT GAAGAACGAC AAGGACAAGT TGGAGAGAGT GCTTGACGTG 
TCTCACCAGC AGATGGAGCA GTACCAGGAG CAGCCAGCTC ATGTCCACAA GATCGCCTAC 
CAGCAGAGGC TGCTGCAGGA GGACCTGGTG ACCATCAGGG CCCAGATTTC CCGCGTCTCA 
ACGGAAATGG CGCGTGCGTG GGAGGAGTAC AACCGGCTGG AGCAATCAGT GGAGCTCCTA 
AGGCTGGCAC TGCAGGCACA CATGGCCCAC AACGACACGT CTCAGCAGGA AAAGGCCGAA 
CTGAAGCGGG AGCTGTGGAG GATTGAGGAC GTCATGGGTG GTCTTAGTGC CAGCAAAGCC 
AACTACAAGA TAACTGTGGA CTCTATTCAG AATCCAGACA GGAGATTAGT GCCTTCGTTG 
TCGGACCAGG CATTGCCTTC CGATGGCGCG GAGGTCCAGC CTCCCCCCCG CAGCTCCATC 
TCCCACACTT TACCTCACAG CACCGTGCCA AAGTGGGCAG AGGATGACGC CCCGCCCAGG 
CCGCCACTGC CTCGTCTCTA TGACTACGAG GAGATGCCCC CCGTTGTGCC CCCGCTCCCC 
AAGGAGGCCT CGGTCATCCG CCACACATCG GTGCGAGGGC TCAAACGCCA GTCGGACGAG 
AGGAAGCGGG ACAGGGAGGG CGGCCAGTAC ATTGCCAATG GAGACTGTAA GACTGATTTG 
AGGTCATACC TGAGCGAACC AGAGCTGCCA GGAAGCAGCC AGCCAATCAC AGCATCTGAA 
GCTGACTACC AGTATTACCC AGGCAAAGGT TTGTTGGGAA CCACGTCTCG GCTGGACCAG 
TCCAACTCCA TCTCGTCCTA CGTCACCTTG AGGAGGGCTC CCGGCAGCGC CGCACCAAAG 
GACCGACCCA AAAGCGCCTT TGAGCGCCTG TCATCCCCCA CGGAGGGCGG CCCGCCCCCC 
AGCAGCCAAA CCCGAGGCCG GATGACTGCC GAGGAGCAGC TGGAGCGCAT GAAGCGCCAC 
CAGAGGGCGC TGGTCCGCGA GCGCAAGAGG AACCTGAGCC ACGGCGAGCG CTCTGGCGGC 
GGGGGCCCGG CAGCATCAGG CTCTCAGCGC TCGTCCTCGG CGTCGTCCCG ACTGCCCTCC 
AGCCCCTGCG TGTTCGACTG GCAGCAGGAG CGACCAGGAG CTGAAGGTCA GAGCGACGAA 
GGCCTGAATC TGGTCAGAGA GAGGGAGACG AGGGACTCTA ATGAGTGGGT GACGGTCACC 
GCCAGGCGCA TCCAGGAGGC CGACCTGGAG CCCCTCGACT ACGACCTGGA CATCAGCCGG 
GAGCTGTCCA AACCGGACAA AGTTGTGATC CCGGAGCGCT ACGTGGAGTC GGACCCAGAG 
GAGCCTCTGA GTCCAGAGGA GCTGGAGGAA CGCGGCCGGC GCGCGGAACG CATCAAGAAC 
CTTCTCACCA GGTCCAGTGT CCAGAACATC CAGCCATCAG CATCGCTGGA ATTCAACGAG 
CTGGACAACG CCATGGAGGA GCAGGAGAGG ATCATGAGCG TCTCCTACGC GCTGGCCTCA 
GAGGCTTCAC GCAAGAGTAA ACTGGTCGCA GCCAAAGCTG CTGCTCAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410794.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409016.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X7, mRNA.

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CloneID	OPo196446
Clone ID Related Accession (Same CDS sequence)	XM_008410794.2
Accession Version	XM_008410794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAATC AGTCCAGCTA CTGCAACCAC ATACCTCACC CTGGATACCC CTTTTACCAT 
GCGCCATGCA GTCATAACCA AAAGAGCACG TCGTTCTGTC ATCCTGTAAC TGGACAGATT 
TCCTCAGAGA ACGTGGACTT CCTCCTGCAG GAACAGTCGC AAGGTCCTCG CATGATGTCC 
AGATTCACGG CTGAGCAGCT CTCCAGCACC ACTGTGAGCG AGGCCTCCAC CGCCATCACC 
TCCTCCACCG TGGACTCCAC CTCTGCCTCC AAGGCATCTC GATCCAGTGG GAAGACTCAC 
AACTTTGGGA AAAGAGAACA ATCCATCAAG AGGAATCCTA ACGTCCCAGT GGTGGTCCGA 
GGATGGCTGT ACAAACAGGA CAGCTCTGGG ATGCGCCTTT GGAAGAGGAA GTGGTTTGTC 
TTGTCTGATT TCTGCCTTTT CTACTACAAA GACAGCAGAG AGGAGGCGGT CTTGGGCAGT 
ATTCCTCTGC CCAGCTATGT CATTTCACCC GTTGGCCCCG AAGATCACAT CAGCCGCAAG 
TATGCCTTCA AGGCTAGCCA CACCGGTATG CGTTCATACA TTTACAAGCA GAGCTCTGTG 
ATTGGCTCGC AGGCAGAGCA CACTGGCATG CGGACGTATT ACTTCAGCGC TGACACCCAG 
GAGGACATGA GCACCTGGCT GAAGGCCATG AACCAGGCAT CGCTGATGCA GAACCACCCC 
GACAGGCTCA GACCCGTTGA CCAAGTCACC TCGTTGTTGC AACAAGCTGT CCCGCAGACA 
AACCATGTGA ATCACCACAA GATGAAGACC GTAGACTCTG AAACCACAAA AGCAATTGTC 
CACGAAGTTC TGCTGGAGCC CATTCACCGT AACACAGAAG AGCTCTGCAG CTTTCACCAG 
GACGCCGGCA ACTTCACCCT GGAGCGCCAC GCGGGGACCG CCTCTTTGGA GATGGACAAA 
CAGAGCCCCC TTCCATCCAG ACCGCCAGCC GCCTCCACAC TCCCTCTGTC GGACCGCGCA 
TCCGCCTCTG CTCCCGTGTC CAGGGTGACG TCCCGCGCTC CGTCCCGCTC CGCCTCGACA 
TTGCCCTCCA GCCTTTGCAT GAGGAACGGC CTGGTGTCCA CGCCCAGCCC CATCCTGGAG 
CCCAACGGCA TCGCGGCGGG GACGTACCAG AGGGCGCCGG AGCCGGCAGC CTCCGACACG 
TTCAAGCATC TGCAGAGGAG GAACACTCTG GAGCAGGTGG AGCAGTGGGT CAAAGTTCAG 
AAGGCTGAGC AAAGGGGCCC GCCCTCCAGA GAAAACACCC TTCCCCGCCG GACGCCGCCC 
ACGCAGAACA AGTACACCTC CATGGATGCC TTTCAAACTT TGCCAAAAAC TCCACGCCAC 
AGCCCCCCAT CGGGTCGACT CGGCGAGTAC AAGTACGCCC ACGACCGCCT GAGCCACTTC 
CGCCTCACCC CAGACCAGGG GGGCACGGGG TCCAACACAG TGCTGCAGCT GTACGAGTGG 
CAGCAGCGCC AACAGTTCCG TCATGGCAGC CCCACGGCGC CAATCTACAC CCCGGCCCCG 
GATTACCCGT TCGGGCCCCG CCCCCCGTCC ACCGTGCCGC CTTCCTCCTT GTTGCGCAGG 
TCGGAGGGGT CTCCTCGCTG CGTGTCGGTG CCACCGTCGG CCGCAGACAT CCCGCCACCG 
GGGCCTCCGC CAGGCACCAG CAGGACGCTG TCCCCCAACC GAAGGCCGCA CACTCCTGCC 
GAACGGGTGA CTGTAAGGCC CGCTGGGGAC GGGCCCCAGC TGAACGTCCC CTTCACCGTG 
TCCCCCCGCA GGAGCAAGTC TCAGATGCTG AAGGCTGCTA CTATTGAGAG ACGCTCGATG 
CCTTCAACTG GCTACATCAC TCACACAGTC AGCGCTCCCA GCCTTCATGG CAAGACGCCG 
GAGGAGCTCA CTCTGCTCCT CATTCAACTG CGCCGCCACC AGGCCAAGAT GGCCGCTGTG 
CGTCAGCAGA CCTTAGCTCA GATCCAGCGG CTGAACGCTC CCAGAGAGTC CTGCCACCAA 
AACCGCCTCC TCACCGCTGT CGCCGTTAGC GACAGCCCCC TCCTCCTCAG CGCCACCGCG 
TCTCCTGAGT CGCACCTCGA AGATGTCGGC CTCTGCAGCG TCCAGGCTGA TGACACGTAC 
ATGCAGCTGA AGAAGGACCT GGAATATCTG GACCTAAAGA TCAGAGCTCT AGAGCCGCTG 
ATCCTCGCAG TTCACAGTTT ACTACTGCAC TGCACTGCTG GGCCACTCAG GCCTTTAATG 
AACGTAGCTG GAAGTCAGGT GCTGAAGGAG GCGGGGAAGC CTGTCAAGGT TGCAGAAAGC 
GATGTGGATG TGAAGTTGAG TCGGTTATGT GAGCAGGACA AGATTCTGAA GGACCTGGAG 
GCGAGGATCA GCTCCCTGAA GAACGACAAG GACAAGTTGG AGAGAGTGCT TGACGTGTCT 
CACCAGCAGA TGGAGCAGTA CCAGGAGCAG CCAGCTCATG TCCACAAGAT CGCCTACCAG 
CAGAGGCTGC TGCAGGAGGA CCTGGTGACC ATCAGGGCCC AGATTTCCCG CGTCTCAACG 
GAAATGGCGC GTGCGTGGGA GGAGTACAAC CGGCTGGAGC AATCAGTGGA GCTCCTAAGG 
CTGGCACTGC AGGCACACAT GGCCCACAAC GACACGTCTC AGCAGGAAAA GGCCGAACTG 
AAGCGGGAGC TGTGGAGGAT TGAGGACGTC ATGGGTGGTC TTAGTGCCAG CAAAGCCAAC 
TACAAGATAA CTGTGGACTC TATTCAGAAT CCAGCAGACA GGAGATTAGT GCCTTCGTTG 
TCGGACCAGG CATTGCCTTC CGATGGCGCG GAGGTCCAGC CTCCCCCCCG CAGCTCCATC 
TCCCACACTT TACCTCACAG CACCGTGCCA AAGTGGGCAG AGGATGACGC CCCGCCCAGG 
CCGCCACTGC CTCGTCTCTA TGACTACGAG GAGATGCCCC CCGTTGTGCC CCCGCTCCCC 
AAGGAGGCCT CGGTCATCCG CCACACATCG GTGCGAGGGC TCAAACGCCA GTCGGACGAG 
AGGAAGCGGG ACAGGGAGGG CGGCCAGTAC ATTGCCAATG GAGACTGTAA GACTGATTTG 
AGGTCATACC TGAGCGAACC AGAGCTGCCA GGAAGCAGCC AGCCAATCAC AGCATCTGAA 
GCTGACTACC AGTATTACCC AGGCAAAGGT TTGTTGGGAA CCACGTCTCG GCTGGACCAG 
TCCAACTCCA TCTCGTCCTA CGTCACCTTG AGGAGGGCTC CCGGCAGCGC CGCACCAAAG 
GACCGACCCA AAAGCGCCTT TGAGCGCCTG TCATCCCCCA CGGAGGGCGG CCCGCCCCCC 
AGCAGCCAAA CCCGAGGCCG GATGACTGCC GAGGAGCAGC TGGAGCGCAT GAAGCGCCAC 
CAGAGGGCGC TGGTCCGCGA GCGCAAGAGG AACCTGAGCC ACGGCGAGCG CTCTGGCGGC 
GGGGGCCCGG CAGCATCAGG CTCTCAGCGC TCGTCCTCGG CGTCGTCCCG ACTGCCCTCC 
AGCCCCTGCG TGTTCGACTG GCAGCAGGAG CGACCAGGAG CTGAAGGTCA GAGCGACGAA 
GGCCTGAATC TGGTCAGAGA GAGGGAGACG AGGGACTCTA ATGAGTGGGT GACGGTCACC 
GCCAGGCGCA TCCAGGAGGC CGACCTGGAG CCCCTCGACT ACGACCTGGA CATCAGCCGG 
GAGCTGTCCA AACCGGACAA AGTTGTGATC CCGGAGCGCT ACGTGGAGTC GGACCCAGAG 
GAGCCTCTGA GTCCAGAGGA GCTGGAGGAA CGCGGCCGGC GCGCGGAACG CATCAAGAAC 
CTTCTCACCA GGTCCAGTGT CCAGAACATC CAGCCATCAG CATCGCTGGA ATTCAACGAG 
CTGGACAACG CCATGGAGGA GCAGGAGAGG ATCATGAGCG TCTCCTACGC GCTGGCCTCA 
GAGGCTTCAC GCAAGAGTAA ACTGGTCGCA GCCAAAGCTG CTGCTCAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409016.1
CDS	75..4088
Translation	MWNQSSYCNHIPHPGYPFYHAPCSHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410794.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410794.2

ORF Insert Sequence:

ATGTGGAATC AGTCCAGCTA CTGCAACCAC ATACCTCACC CTGGATACCC CTTTTACCAT 
GCGCCATGCA GTCATAACCA AAAGAGCACG TCGTTCTGTC ATCCTGTAAC TGGACAGATT 
TCCTCAGAGA ACGTGGACTT CCTCCTGCAG GAACAGTCGC AAGGTCCTCG CATGATGTCC 
AGATTCACGG CTGAGCAGCT CTCCAGCACC ACTGTGAGCG AGGCCTCCAC CGCCATCACC 
TCCTCCACCG TGGACTCCAC CTCTGCCTCC AAGGCATCTC GATCCAGTGG GAAGACTCAC 
AACTTTGGGA AAAGAGAACA ATCCATCAAG AGGAATCCTA ACGTCCCAGT GGTGGTCCGA 
GGATGGCTGT ACAAACAGGA CAGCTCTGGG ATGCGCCTTT GGAAGAGGAA GTGGTTTGTC 
TTGTCTGATT TCTGCCTTTT CTACTACAAA GACAGCAGAG AGGAGGCGGT CTTGGGCAGT 
ATTCCTCTGC CCAGCTATGT CATTTCACCC GTTGGCCCCG AAGATCACAT CAGCCGCAAG 
TATGCCTTCA AGGCTAGCCA CACCGGTATG CGTTCATACA TTTACAAGCA GAGCTCTGTG 
ATTGGCTCGC AGGCAGAGCA CACTGGCATG CGGACGTATT ACTTCAGCGC TGACACCCAG 
GAGGACATGA GCACCTGGCT GAAGGCCATG AACCAGGCAT CGCTGATGCA GAACCACCCC 
GACAGGCTCA GACCCGTTGA CCAAGTCACC TCGTTGTTGC AACAAGCTGT CCCGCAGACA 
AACCATGTGA ATCACCACAA GATGAAGACC GTAGACTCTG AAACCACAAA AGCAATTGTC 
CACGAAGTTC TGCTGGAGCC CATTCACCGT AACACAGAAG AGCTCTGCAG CTTTCACCAG 
GACGCCGGCA ACTTCACCCT GGAGCGCCAC GCGGGGACCG CCTCTTTGGA GATGGACAAA 
CAGAGCCCCC TTCCATCCAG ACCGCCAGCC GCCTCCACAC TCCCTCTGTC GGACCGCGCA 
TCCGCCTCTG CTCCCGTGTC CAGGGTGACG TCCCGCGCTC CGTCCCGCTC CGCCTCGACA 
TTGCCCTCCA GCCTTTGCAT GAGGAACGGC CTGGTGTCCA CGCCCAGCCC CATCCTGGAG 
CCCAACGGCA TCGCGGCGGG GACGTACCAG AGGGCGCCGG AGCCGGCAGC CTCCGACACG 
TTCAAGCATC TGCAGAGGAG GAACACTCTG GAGCAGGTGG AGCAGTGGGT CAAAGTTCAG 
AAGGCTGAGC AAAGGGGCCC GCCCTCCAGA GAAAACACCC TTCCCCGCCG GACGCCGCCC 
ACGCAGAACA AGTACACCTC CATGGATGCC TTTCAAACTT TGCCAAAAAC TCCACGCCAC 
AGCCCCCCAT CGGGTCGACT CGGCGAGTAC AAGTACGCCC ACGACCGCCT GAGCCACTTC 
CGCCTCACCC CAGACCAGGG GGGCACGGGG TCCAACACAG TGCTGCAGCT GTACGAGTGG 
CAGCAGCGCC AACAGTTCCG TCATGGCAGC CCCACGGCGC CAATCTACAC CCCGGCCCCG 
GATTACCCGT TCGGGCCCCG CCCCCCGTCC ACCGTGCCGC CTTCCTCCTT GTTGCGCAGG 
TCGGAGGGGT CTCCTCGCTG CGTGTCGGTG CCACCGTCGG CCGCAGACAT CCCGCCACCG 
GGGCCTCCGC CAGGCACCAG CAGGACGCTG TCCCCCAACC GAAGGCCGCA CACTCCTGCC 
GAACGGGTGA CTGTAAGGCC CGCTGGGGAC GGGCCCCAGC TGAACGTCCC CTTCACCGTG 
TCCCCCCGCA GGAGCAAGTC TCAGATGCTG AAGGCTGCTA CTATTGAGAG ACGCTCGATG 
CCTTCAACTG GCTACATCAC TCACACAGTC AGCGCTCCCA GCCTTCATGG CAAGACGCCG 
GAGGAGCTCA CTCTGCTCCT CATTCAACTG CGCCGCCACC AGGCCAAGAT GGCCGCTGTG 
CGTCAGCAGA CCTTAGCTCA GATCCAGCGG CTGAACGCTC CCAGAGAGTC CTGCCACCAA 
AACCGCCTCC TCACCGCTGT CGCCGTTAGC GACAGCCCCC TCCTCCTCAG CGCCACCGCG 
TCTCCTGAGT CGCACCTCGA AGATGTCGGC CTCTGCAGCG TCCAGGCTGA TGACACGTAC 
ATGCAGCTGA AGAAGGACCT GGAATATCTG GACCTAAAGA TCAGAGCTCT AGAGCCGCTG 
ATCCTCGCAG TTCACAGTTT ACTACTGCAC TGCACTGCTG GGCCACTCAG GCCTTTAATG 
AACGTAGCTG GAAGTCAGGT GCTGAAGGAG GCGGGGAAGC CTGTCAAGGT TGCAGAAAGC 
GATGTGGATG TGAAGTTGAG TCGGTTATGT GAGCAGGACA AGATTCTGAA GGACCTGGAG 
GCGAGGATCA GCTCCCTGAA GAACGACAAG GACAAGTTGG AGAGAGTGCT TGACGTGTCT 
CACCAGCAGA TGGAGCAGTA CCAGGAGCAG CCAGCTCATG TCCACAAGAT CGCCTACCAG 
CAGAGGCTGC TGCAGGAGGA CCTGGTGACC ATCAGGGCCC AGATTTCCCG CGTCTCAACG 
GAAATGGCGC GTGCGTGGGA GGAGTACAAC CGGCTGGAGC AATCAGTGGA GCTCCTAAGG 
CTGGCACTGC AGGCACACAT GGCCCACAAC GACACGTCTC AGCAGGAAAA GGCCGAACTG 
AAGCGGGAGC TGTGGAGGAT TGAGGACGTC ATGGGTGGTC TTAGTGCCAG CAAAGCCAAC 
TACAAGATAA CTGTGGACTC TATTCAGAAT CCAGCAGACA GGAGATTAGT GCCTTCGTTG 
TCGGACCAGG CATTGCCTTC CGATGGCGCG GAGGTCCAGC CTCCCCCCCG CAGCTCCATC 
TCCCACACTT TACCTCACAG CACCGTGCCA AAGTGGGCAG AGGATGACGC CCCGCCCAGG 
CCGCCACTGC CTCGTCTCTA TGACTACGAG GAGATGCCCC CCGTTGTGCC CCCGCTCCCC 
AAGGAGGCCT CGGTCATCCG CCACACATCG GTGCGAGGGC TCAAACGCCA GTCGGACGAG 
AGGAAGCGGG ACAGGGAGGG CGGCCAGTAC ATTGCCAATG GAGACTGTAA GACTGATTTG 
AGGTCATACC TGAGCGAACC AGAGCTGCCA GGAAGCAGCC AGCCAATCAC AGCATCTGAA 
GCTGACTACC AGTATTACCC AGGCAAAGGT TTGTTGGGAA CCACGTCTCG GCTGGACCAG 
TCCAACTCCA TCTCGTCCTA CGTCACCTTG AGGAGGGCTC CCGGCAGCGC CGCACCAAAG 
GACCGACCCA AAAGCGCCTT TGAGCGCCTG TCATCCCCCA CGGAGGGCGG CCCGCCCCCC 
AGCAGCCAAA CCCGAGGCCG GATGACTGCC GAGGAGCAGC TGGAGCGCAT GAAGCGCCAC 
CAGAGGGCGC TGGTCCGCGA GCGCAAGAGG AACCTGAGCC ACGGCGAGCG CTCTGGCGGC 
GGGGGCCCGG CAGCATCAGG CTCTCAGCGC TCGTCCTCGG CGTCGTCCCG ACTGCCCTCC 
AGCCCCTGCG TGTTCGACTG GCAGCAGGAG CGACCAGGAG CTGAAGGTCA GAGCGACGAA 
GGCCTGAATC TGGTCAGAGA GAGGGAGACG AGGGACTCTA ATGAGTGGGT GACGGTCACC 
GCCAGGCGCA TCCAGGAGGC CGACCTGGAG CCCCTCGACT ACGACCTGGA CATCAGCCGG 
GAGCTGTCCA AACCGGACAA AGTTGTGATC CCGGAGCGCT ACGTGGAGTC GGACCCAGAG 
GAGCCTCTGA GTCCAGAGGA GCTGGAGGAA CGCGGCCGGC GCGCGGAACG CATCAAGAAC 
CTTCTCACCA GGTCCAGTGT CCAGAACATC CAGCCATCAG CATCGCTGGA ATTCAACGAG 
CTGGACAACG CCATGGAGGA GCAGGAGAGG ATCATGAGCG TCTCCTACGC GCTGGCCTCA 
GAGGCTTCAC GCAAGAGTAA ACTGGTCGCA GCCAAAGCTG CTGCTCAGCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_017305316.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017160805.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OPo196447
Clone ID Related Accession (Same CDS sequence)	XM_017305316.1
Accession Version	XM_017305316.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGGTGA AGTTGAGTCG GTTATGTGAG CAGGACAAGA TTCTGAAGGA CCTGGAGGCG 
AGGATCAGCT CCCTGAAGAA CGACAAGGAC AAGTTGGAGA GAGTGCTTGA CGTGTCTCAC 
CAGCAGATGG AGCAGTACCA GGAGCAGCCA GCTCATGTCC ACAAGATCGC CTACCAGCAG 
AGGCTGCTGC AGGAGGACCT GGTGACCATC AGGGCCCAGA TTTCCCGCGT CTCAACGGAA 
ATGGCGCGTG CGTGGGAGGA GTACAACCGG CTGGAGCAAT CAGTGGAGCT CCTAAGGCTG 
GCACTGCAGG CACACATGGC CCACAACGAC ACGTCTCAGC AGGAAAAGGC CGAACTGAAG 
CGGGAGCTGT GGAGGATTGA GGACGTCATG GGTGGTCTTA GTGCCAGCAA AGCCAACTAC 
AAGATAACTG TGGACTCTAT TCAGAATCCA GCAGACAGGA GATTAGTGCC TTCGTTGTCG 
GACCAGGCAT TGCCTTCCGA TGGCGCGGAG GTCCAGCCTC CCCCCCGCAG CTCCATCTCC 
CACACTTTAC CTCACAGCAC CGTGCCAAAG TGGGCAGAGG ATGACGCCCC GCCCAGGCCG 
CCACTGCCTC GTCTCTATGA CTACGAGGAG ATGCCCCCCG TTGTGCCCCC GCTCCCCAAG 
GAGGCCTCGG TCATCCGCCA CACATCGGTG CGAGGGCTCA AACGCCAGTC GGACGAGAGG 
AAGCGGGACA GGGAGGGCGG CCAGTACATT GCCAATGGAG ACTGTAAGAC TGATTTGAGG 
TCATACCTGA GCGAACCAGA GCTGCCAGGA AGCAGCCAGC CAATCACAGC ATCTGAAGCT 
GACTACCAGT ATTACCCAGG CAAAGGTTTG TTGGGAACCA CGTCTCGGCT GGACCAGTCC 
AACTCCATCT CGTCCTACGT CACCTTGAGG AGGGCTCCCG GCAGCGCCGC ACCAAAGGAC 
CGACCCAAAA GCGCCTTTGA GCGCCTGTCA TCCCCCACGG AGGGCGGCCC GCCCCCCAGC 
AGCCAAACCC GAGGCCGGAT GACTGCCGAG GAGCAGCTGG AGCGCATGAA GCGCCACCAG 
AGGGCGCTGG TCCGCGAGCG CAAGAGGAAC CTGAGCCACG GCGAGCGCTC TGGCGGCGGG 
GGCCCGGCAG CATCAGGCTC TCAGCGCTCG TCCTCGGCGT CGTCCCGACT GCCCTCCAGC 
CCCTGCGTGT TCGACTGGCA GCAGGAGCGA CCAGGAGCTG AAGGTCAGAG CGACGAAGGC 
CTGAATCTGG TCAGAGAGAG GGAGACGAGG GACTCTAATG AGTGGGTGAC GGTCACCGCC 
AGGCGCATCC AGGAGGCCGA CCTGGAGCCC CTCGACTACG ACCTGGACAT CAGCCGGGAG 
CTGTCCAAAC CGGACAAAGT TGTGATCCCG GAGCGCTACG TGGAGTCGGA CCCAGAGGAG 
CCTCTGAGTC CAGAGGAGCT GGAGGAACGC GGCCGGCGCG CGGAACGCAT CAAGAACCTT 
CTCACCAGGT CCAGTGTCCA GAACATCCAG CCATCAGCAT CGCTGGAATT CAACGAGCTG 
GACAACGCCA TGGAGGAGCA GGAGAGGATC ATGAGCGTCT CCTACGCGCT GGCCTCAGAG 
GCTTCACGCA AGAGTAAACT GGTCGCAGCC AAAGCTGCTG CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017160805.1
CDS	170..4180
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_017305316.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017305316.1

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGCCGGAG GAGCTCACTC TGCTCCTCAT TCAACTGCGC 
CGCCACCAGG CCAAGATGGC CGCTGTGCGT CAGCAGACCT TAGCTCAGAT CCAGCGGCTG 
AACGCTCCCA GAGAGTCCTG CCACCAAAAC CGCCTCCTCA CCGCTGTCGC CGTTAGCGAC 
AGCCCCCTCC TCCTCAGCGC CACCGCGTCT CCTGAGTCGC ACCTCGAAGA TGTCGGCCTC 
TGCAGCGTCC AGGCTGATGA CACGTACATG CAGCTGAAGA AGGACCTGGA ATATCTGGAC 
CTAAAGGTGA AGTTGAGTCG GTTATGTGAG CAGGACAAGA TTCTGAAGGA CCTGGAGGCG 
AGGATCAGCT CCCTGAAGAA CGACAAGGAC AAGTTGGAGA GAGTGCTTGA CGTGTCTCAC 
CAGCAGATGG AGCAGTACCA GGAGCAGCCA GCTCATGTCC ACAAGATCGC CTACCAGCAG 
AGGCTGCTGC AGGAGGACCT GGTGACCATC AGGGCCCAGA TTTCCCGCGT CTCAACGGAA 
ATGGCGCGTG CGTGGGAGGA GTACAACCGG CTGGAGCAAT CAGTGGAGCT CCTAAGGCTG 
GCACTGCAGG CACACATGGC CCACAACGAC ACGTCTCAGC AGGAAAAGGC CGAACTGAAG 
CGGGAGCTGT GGAGGATTGA GGACGTCATG GGTGGTCTTA GTGCCAGCAA AGCCAACTAC 
AAGATAACTG TGGACTCTAT TCAGAATCCA GCAGACAGGA GATTAGTGCC TTCGTTGTCG 
GACCAGGCAT TGCCTTCCGA TGGCGCGGAG GTCCAGCCTC CCCCCCGCAG CTCCATCTCC 
CACACTTTAC CTCACAGCAC CGTGCCAAAG TGGGCAGAGG ATGACGCCCC GCCCAGGCCG 
CCACTGCCTC GTCTCTATGA CTACGAGGAG ATGCCCCCCG TTGTGCCCCC GCTCCCCAAG 
GAGGCCTCGG TCATCCGCCA CACATCGGTG CGAGGGCTCA AACGCCAGTC GGACGAGAGG 
AAGCGGGACA GGGAGGGCGG CCAGTACATT GCCAATGGAG ACTGTAAGAC TGATTTGAGG 
TCATACCTGA GCGAACCAGA GCTGCCAGGA AGCAGCCAGC CAATCACAGC ATCTGAAGCT 
GACTACCAGT ATTACCCAGG CAAAGGTTTG TTGGGAACCA CGTCTCGGCT GGACCAGTCC 
AACTCCATCT CGTCCTACGT CACCTTGAGG AGGGCTCCCG GCAGCGCCGC ACCAAAGGAC 
CGACCCAAAA GCGCCTTTGA GCGCCTGTCA TCCCCCACGG AGGGCGGCCC GCCCCCCAGC 
AGCCAAACCC GAGGCCGGAT GACTGCCGAG GAGCAGCTGG AGCGCATGAA GCGCCACCAG 
AGGGCGCTGG TCCGCGAGCG CAAGAGGAAC CTGAGCCACG GCGAGCGCTC TGGCGGCGGG 
GGCCCGGCAG CATCAGGCTC TCAGCGCTCG TCCTCGGCGT CGTCCCGACT GCCCTCCAGC 
CCCTGCGTGT TCGACTGGCA GCAGGAGCGA CCAGGAGCTG AAGGTCAGAG CGACGAAGGC 
CTGAATCTGG TCAGAGAGAG GGAGACGAGG GACTCTAATG AGTGGGTGAC GGTCACCGCC 
AGGCGCATCC AGGAGGCCGA CCTGGAGCCC CTCGACTACG ACCTGGACAT CAGCCGGGAG 
CTGTCCAAAC CGGACAAAGT TGTGATCCCG GAGCGCTACG TGGAGTCGGA CCCAGAGGAG 
CCTCTGAGTC CAGAGGAGCT GGAGGAACGC GGCCGGCGCG CGGAACGCAT CAAGAACCTT 
CTCACCAGGT CCAGTGTCCA GAACATCCAG CCATCAGCAT CGCTGGAATT CAACGAGCTG 
GACAACGCCA TGGAGGAGCA GGAGAGGATC ATGAGCGTCT CCTACGCGCT GGCCTCAGAG 
GCTTCACGCA AGAGTAAACT GGTCGCAGCC AAAGCTGCTG CTCAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410795.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409017.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OPo196448
Clone ID Related Accession (Same CDS sequence)	XM_008410795.2
Accession Version	XM_008410795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3933bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGGCTGAT GACACGTACA TGCAGCTGAA GAAGGACCTG 
GAATATCTGG ACCTAAAGAT CAGAGCTCTA GAGCCGCTGA TCCTCGCAGT TCACAGTTTA 
CTACTGCACT GCACTGCTGG GCCACTCAGG CCTTTAATGA ACGTAGCTGG AAGTCAGGTG 
CTGAAGGAGG CGGGGAAGCC TGTCAAGGTT GCAGAAAGCG ATGTGGATGT GAAGTTGAGT 
CGGTTATGTG AGCAGGACAA GATTCTGAAG GACCTGGAGG CGAGGATCAG CTCCCTGAAG 
AACGACAAGG ACAAGTTGGA GAGAGTGCTT GACGTGTCTC ACCAGCAGAT GGAGCAGTAC 
CAGGAGCAGC CAGCTCATGT CCACAAGATC GCCTACCAGC AGAGGCTGCT GCAGGAGGAC 
CTGGTGACCA TCAGGGCCCA GATTTCCCGC GTCTCAACGG AAATGGCGCG TGCGTGGGAG 
GAGTACAACC GGCTGGAGCA ATCAGTGGAG CTCCTAAGGC TGGCACTGCA GGCACACATG 
GCCCACAACG ACACGTCTCA GCAGGAAAAG GCCGAACTGA AGCGGGAGCT GTGGAGGATT 
GAGGACGTCA TGGGTGGTCT TAGTGCCAGC AAAGCCAACT ACAAGATAAC TGTGGACTCT 
ATTCAGAATC CAGCAGACAG GAGATTAGTG CCTTCGTTGT CGGACCAGGC ATTGCCTTCC 
GATGGCGCGG AGGTCCAGCC TCCCCCCCGC AGCTCCATCT CCCACACTTT ACCTCACAGC 
ACCGTGCCAA AGTGGGCAGA GGATGACGCC CCGCCCAGGC CGCCACTGCC TCGTCTCTAT 
GACTACGAGG AGATGCCCCC CGTTGTGCCC CCGCTCCCCA AGGAGGCCTC GGTCATCCGC 
CACACATCGG TGCGAGGGCT CAAACGCCAG TCGGACGAGA GGAAGCGGGA CAGGGAGGGC 
GGCCAGTACA TTGCCAATGG AGACTGTAAG ACTGATTTGA GGTCATACCT GAGCGAACCA 
GAGCTGCCAG GAAGCAGCCA GCCAATCACA GCATCTGAAG CTGACTACCA GTATTACCCA 
GGCAAAGGTT TGTTGGGAAC CACGTCTCGG CTGGACCAGT CCAACTCCAT CTCGTCCTAC 
GTCACCTTGA GGAGGGCTCC CGGCAGCGCC GCACCAAAGG ACCGACCCAA AAGCGCCTTT 
GAGCGCCTGT CATCCCCCAC GGAGGGCGGC CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG 
ATGACTGCCG AGGAGCAGCT GGAGCGCATG AAGCGCCACC AGAGGGCGCT GGTCCGCGAG 
CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC TCTGGCGGCG GGGGCCCGGC AGCATCAGGC 
TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA CTGCCCTCCA GCCCCTGCGT GTTCGACTGG 
CAGCAGGAGC GACCAGGAGC TGAAGGTCAG AGCGACGAAG GCCTGAATCT GGTCAGAGAG 
AGGGAGACGA GGGACTCTAA TGAGTGGGTG ACGGTCACCG CCAGGCGCAT CCAGGAGGCC 
GACCTGGAGC CCCTCGACTA CGACCTGGAC ATCAGCCGGG AGCTGTCCAA ACCGGACAAA 
GTTGTGATCC CGGAGCGCTA CGTGGAGTCG GACCCAGAGG AGCCTCTGAG TCCAGAGGAG 
CTGGAGGAAC GCGGCCGGCG CGCGGAACGC ATCAAGAACC TTCTCACCAG GTCCAGTGTC 
CAGAACATCC AGCCATCAGC ATCGCTGGAA TTCAACGAGC TGGACAACGC CATGGAGGAG 
CAGGAGAGGA TCATGAGCGT CTCCTACGCG CTGGCCTCAG AGGCTTCACG CAAGAGTAAA 
CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409017.1
CDS	166..4098
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410795.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410795.2

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGGCTGAT GACACGTACA TGCAGCTGAA GAAGGACCTG 
GAATATCTGG ACCTAAAGAT CAGAGCTCTA GAGCCGCTGA TCCTCGCAGT TCACAGTTTA 
CTACTGCACT GCACTGCTGG GCCACTCAGG CCTTTAATGA ACGTAGCTGG AAGTCAGGTG 
CTGAAGGAGG CGGGGAAGCC TGTCAAGGTT GCAGAAAGCG ATGTGGATGT GAAGTTGAGT 
CGGTTATGTG AGCAGGACAA GATTCTGAAG GACCTGGAGG CGAGGATCAG CTCCCTGAAG 
AACGACAAGG ACAAGTTGGA GAGAGTGCTT GACGTGTCTC ACCAGCAGAT GGAGCAGTAC 
CAGGAGCAGC CAGCTCATGT CCACAAGATC GCCTACCAGC AGAGGCTGCT GCAGGAGGAC 
CTGGTGACCA TCAGGGCCCA GATTTCCCGC GTCTCAACGG AAATGGCGCG TGCGTGGGAG 
GAGTACAACC GGCTGGAGCA ATCAGTGGAG CTCCTAAGGC TGGCACTGCA GGCACACATG 
GCCCACAACG ACACGTCTCA GCAGGAAAAG GCCGAACTGA AGCGGGAGCT GTGGAGGATT 
GAGGACGTCA TGGGTGGTCT TAGTGCCAGC AAAGCCAACT ACAAGATAAC TGTGGACTCT 
ATTCAGAATC CAGCAGACAG GAGATTAGTG CCTTCGTTGT CGGACCAGGC ATTGCCTTCC 
GATGGCGCGG AGGTCCAGCC TCCCCCCCGC AGCTCCATCT CCCACACTTT ACCTCACAGC 
ACCGTGCCAA AGTGGGCAGA GGATGACGCC CCGCCCAGGC CGCCACTGCC TCGTCTCTAT 
GACTACGAGG AGATGCCCCC CGTTGTGCCC CCGCTCCCCA AGGAGGCCTC GGTCATCCGC 
CACACATCGG TGCGAGGGCT CAAACGCCAG TCGGACGAGA GGAAGCGGGA CAGGGAGGGC 
GGCCAGTACA TTGCCAATGG AGACTGTAAG ACTGATTTGA GGTCATACCT GAGCGAACCA 
GAGCTGCCAG GAAGCAGCCA GCCAATCACA GCATCTGAAG CTGACTACCA GTATTACCCA 
GGCAAAGGTT TGTTGGGAAC CACGTCTCGG CTGGACCAGT CCAACTCCAT CTCGTCCTAC 
GTCACCTTGA GGAGGGCTCC CGGCAGCGCC GCACCAAAGG ACCGACCCAA AAGCGCCTTT 
GAGCGCCTGT CATCCCCCAC GGAGGGCGGC CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG 
ATGACTGCCG AGGAGCAGCT GGAGCGCATG AAGCGCCACC AGAGGGCGCT GGTCCGCGAG 
CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC TCTGGCGGCG GGGGCCCGGC AGCATCAGGC 
TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA CTGCCCTCCA GCCCCTGCGT GTTCGACTGG 
CAGCAGGAGC GACCAGGAGC TGAAGGTCAG AGCGACGAAG GCCTGAATCT GGTCAGAGAG 
AGGGAGACGA GGGACTCTAA TGAGTGGGTG ACGGTCACCG CCAGGCGCAT CCAGGAGGCC 
GACCTGGAGC CCCTCGACTA CGACCTGGAC ATCAGCCGGG AGCTGTCCAA ACCGGACAAA 
GTTGTGATCC CGGAGCGCTA CGTGGAGTCG GACCCAGAGG AGCCTCTGAG TCCAGAGGAG 
CTGGAGGAAC GCGGCCGGCG CGCGGAACGC ATCAAGAACC TTCTCACCAG GTCCAGTGTC 
CAGAACATCC AGCCATCAGC ATCGCTGGAA TTCAACGAGC TGGACAACGC CATGGAGGAG 
CAGGAGAGGA TCATGAGCGT CTCCTACGCG CTGGCCTCAG AGGCTTCACG CAAGAGTAAA 
CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410797.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409019.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X10, mRNA.

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CloneID	OPo196449
Clone ID Related Accession (Same CDS sequence)	XM_008410797.2
Accession Version	XM_008410797.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3849bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGGCTGAT GACACGTACA TGCAGCTGAA GAAGGACCTG 
GAATATCTGG ACCTAAAGGT AGCTGGAAGT CAGGTGCTGA AGGAGGCGGG GAAGCCTGTC 
AAGGTTGCAG AAAGCGATGT GGATGTGAAG TTGAGTCGGT TATGTGAGCA GGACAAGATT 
CTGAAGGACC TGGAGGCGAG GATCAGCTCC CTGAAGAACG ACAAGGACAA GTTGGAGAGA 
GTGCTTGACG TGTCTCACCA GCAGATGGAG CAGTACCAGG AGCAGCCAGC TCATGTCCAC 
AAGATCGCCT ACCAGCAGAG GCTGCTGCAG GAGGACCTGG TGACCATCAG GGCCCAGATT 
TCCCGCGTCT CAACGGAAAT GGCGCGTGCG TGGGAGGAGT ACAACCGGCT GGAGCAATCA 
GTGGAGCTCC TAAGGCTGGC ACTGCAGGCA CACATGGCCC ACAACGACAC GTCTCAGCAG 
GAAAAGGCCG AACTGAAGCG GGAGCTGTGG AGGATTGAGG ACGTCATGGG TGGTCTTAGT 
GCCAGCAAAG CCAACTACAA GATAACTGTG GACTCTATTC AGAATCCAGC AGACAGGAGA 
TTAGTGCCTT CGTTGTCGGA CCAGGCATTG CCTTCCGATG GCGCGGAGGT CCAGCCTCCC 
CCCCGCAGCT CCATCTCCCA CACTTTACCT CACAGCACCG TGCCAAAGTG GGCAGAGGAT 
GACGCCCCGC CCAGGCCGCC ACTGCCTCGT CTCTATGACT ACGAGGAGAT GCCCCCCGTT 
GTGCCCCCGC TCCCCAAGGA GGCCTCGGTC ATCCGCCACA CATCGGTGCG AGGGCTCAAA 
CGCCAGTCGG ACGAGAGGAA GCGGGACAGG GAGGGCGGCC AGTACATTGC CAATGGAGAC 
TGTAAGACTG ATTTGAGGTC ATACCTGAGC GAACCAGAGC TGCCAGGAAG CAGCCAGCCA 
ATCACAGCAT CTGAAGCTGA CTACCAGTAT TACCCAGGCA AAGGTTTGTT GGGAACCACG 
TCTCGGCTGG ACCAGTCCAA CTCCATCTCG TCCTACGTCA CCTTGAGGAG GGCTCCCGGC 
AGCGCCGCAC CAAAGGACCG ACCCAAAAGC GCCTTTGAGC GCCTGTCATC CCCCACGGAG 
GGCGGCCCGC CCCCCAGCAG CCAAACCCGA GGCCGGATGA CTGCCGAGGA GCAGCTGGAG 
CGCATGAAGC GCCACCAGAG GGCGCTGGTC CGCGAGCGCA AGAGGAACCT GAGCCACGGC 
GAGCGCTCTG GCGGCGGGGG CCCGGCAGCA TCAGGCTCTC AGCGCTCGTC CTCGGCGTCG 
TCCCGACTGC CCTCCAGCCC CTGCGTGTTC GACTGGCAGC AGGAGCGACC AGGAGCTGAA 
GGTCAGAGCG ACGAAGGCCT GAATCTGGTC AGAGAGAGGG AGACGAGGGA CTCTAATGAG 
TGGGTGACGG TCACCGCCAG GCGCATCCAG GAGGCCGACC TGGAGCCCCT CGACTACGAC 
CTGGACATCA GCCGGGAGCT GTCCAAACCG GACAAAGTTG TGATCCCGGA GCGCTACGTG 
GAGTCGGACC CAGAGGAGCC TCTGAGTCCA GAGGAGCTGG AGGAACGCGG CCGGCGCGCG 
GAACGCATCA AGAACCTTCT CACCAGGTCC AGTGTCCAGA ACATCCAGCC ATCAGCATCG 
CTGGAATTCA ACGAGCTGGA CAACGCCATG GAGGAGCAGG AGAGGATCAT GAGCGTCTCC 
TACGCGCTGG CCTCAGAGGC TTCACGCAAG AGTAAACTGG TCGCAGCCAA AGCTGCTGCT 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409019.1
CDS	164..4012
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTADDTYMQLKKDLEYLDLKVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410797.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410797.2

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGGCTGAT GACACGTACA TGCAGCTGAA GAAGGACCTG 
GAATATCTGG ACCTAAAGGT AGCTGGAAGT CAGGTGCTGA AGGAGGCGGG GAAGCCTGTC 
AAGGTTGCAG AAAGCGATGT GGATGTGAAG TTGAGTCGGT TATGTGAGCA GGACAAGATT 
CTGAAGGACC TGGAGGCGAG GATCAGCTCC CTGAAGAACG ACAAGGACAA GTTGGAGAGA 
GTGCTTGACG TGTCTCACCA GCAGATGGAG CAGTACCAGG AGCAGCCAGC TCATGTCCAC 
AAGATCGCCT ACCAGCAGAG GCTGCTGCAG GAGGACCTGG TGACCATCAG GGCCCAGATT 
TCCCGCGTCT CAACGGAAAT GGCGCGTGCG TGGGAGGAGT ACAACCGGCT GGAGCAATCA 
GTGGAGCTCC TAAGGCTGGC ACTGCAGGCA CACATGGCCC ACAACGACAC GTCTCAGCAG 
GAAAAGGCCG AACTGAAGCG GGAGCTGTGG AGGATTGAGG ACGTCATGGG TGGTCTTAGT 
GCCAGCAAAG CCAACTACAA GATAACTGTG GACTCTATTC AGAATCCAGC AGACAGGAGA 
TTAGTGCCTT CGTTGTCGGA CCAGGCATTG CCTTCCGATG GCGCGGAGGT CCAGCCTCCC 
CCCCGCAGCT CCATCTCCCA CACTTTACCT CACAGCACCG TGCCAAAGTG GGCAGAGGAT 
GACGCCCCGC CCAGGCCGCC ACTGCCTCGT CTCTATGACT ACGAGGAGAT GCCCCCCGTT 
GTGCCCCCGC TCCCCAAGGA GGCCTCGGTC ATCCGCCACA CATCGGTGCG AGGGCTCAAA 
CGCCAGTCGG ACGAGAGGAA GCGGGACAGG GAGGGCGGCC AGTACATTGC CAATGGAGAC 
TGTAAGACTG ATTTGAGGTC ATACCTGAGC GAACCAGAGC TGCCAGGAAG CAGCCAGCCA 
ATCACAGCAT CTGAAGCTGA CTACCAGTAT TACCCAGGCA AAGGTTTGTT GGGAACCACG 
TCTCGGCTGG ACCAGTCCAA CTCCATCTCG TCCTACGTCA CCTTGAGGAG GGCTCCCGGC 
AGCGCCGCAC CAAAGGACCG ACCCAAAAGC GCCTTTGAGC GCCTGTCATC CCCCACGGAG 
GGCGGCCCGC CCCCCAGCAG CCAAACCCGA GGCCGGATGA CTGCCGAGGA GCAGCTGGAG 
CGCATGAAGC GCCACCAGAG GGCGCTGGTC CGCGAGCGCA AGAGGAACCT GAGCCACGGC 
GAGCGCTCTG GCGGCGGGGG CCCGGCAGCA TCAGGCTCTC AGCGCTCGTC CTCGGCGTCG 
TCCCGACTGC CCTCCAGCCC CTGCGTGTTC GACTGGCAGC AGGAGCGACC AGGAGCTGAA 
GGTCAGAGCG ACGAAGGCCT GAATCTGGTC AGAGAGAGGG AGACGAGGGA CTCTAATGAG 
TGGGTGACGG TCACCGCCAG GCGCATCCAG GAGGCCGACC TGGAGCCCCT CGACTACGAC 
CTGGACATCA GCCGGGAGCT GTCCAAACCG GACAAAGTTG TGATCCCGGA GCGCTACGTG 
GAGTCGGACC CAGAGGAGCC TCTGAGTCCA GAGGAGCTGG AGGAACGCGG CCGGCGCGCG 
GAACGCATCA AGAACCTTCT CACCAGGTCC AGTGTCCAGA ACATCCAGCC ATCAGCATCG 
CTGGAATTCA ACGAGCTGGA CAACGCCATG GAGGAGCAGG AGAGGATCAT GAGCGTCTCC 
TACGCGCTGG CCTCAGAGGC TTCACGCAAG AGTAAACTGG TCGCAGCCAA AGCTGCTGCT 
CAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410799.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409021.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OPo196450
Clone ID Related Accession (Same CDS sequence)	XM_008410799.2 , XM_008410801.1 , XM_008410800.2
Accession Version	XM_008410799.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410799.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCA GATTCACGGC TGAGCAGCTC TCCAGCACCA CTGTGAGCGA GGCCTCCACC 
GCCATCACCT CCTCCACCGT GGACTCCACC TCTGCCTCCA AGGCATCTCG ATCCAGTGGG 
AAGACTCACA ACTTTGGGAA AAGAGAACAA TCCATCAAGA GGAATCCTAA CGTCCCAGTG 
GTGGTCCGAG GATGGCTGTA CAAACAGGAC AGCTCTGGGA TGCGCCTTTG GAAGAGGAAG 
TGGTTTGTCT TGTCTGATTT CTGCCTTTTC TACTACAAAG ACAGCAGAGA GGAGGCGGTC 
TTGGGCAGTA TTCCTCTGCC CAGCTATGTC ATTTCACCCG TTGGCCCCGA AGATCACATC 
AGCCGCAAGT ATGCCTTCAA GGCTAGCCAC ACCGGTATGC GTTCATACAT TTACAAGCAG 
AGCTCTGTGA TTGGCTCGCA GGCAGAGCAC ACTGGCATGC GGACGTATTA CTTCAGCGCT 
GACACCCAGG AGGACATGAG CACCTGGCTG AAGGCCATGA ACCAGGCATC GCTGATGCAG 
AACCACCCCG ACAGGCTCAG ACCCGTTGAC CAAGTCACCT CGTTGTTGCA ACAAGCTGTC 
CCGCAGACAA ACCATGTGAA TCACCACAAG ATGAAGACCG TAGACTCTGA AACCACAAAA 
GCAATTGTCC ACGAAGTTCT GCTGGAGCCC ATTCACCGTA ACACAGAAGA GCTCTGCAGC 
TTTCACCAGG ACGCCGGCAA CTTCACCCTG GAGCGCCACG CGGGGACCGC CTCTTTGGAG 
ATGGACAAAC AGAGCCCCCT TCCATCCAGA CCGCCAGCCG CCTCCACACT CCCTCTGTCG 
GACCGCGCAT CCGCCTCTGC TCCCGTGTCC AGGGTGACGT CCCGCGCTCC GTCCCGCTCC 
GCCTCGACAT TGCCCTCCAG CCTTTGCATG AGGAACGGCC TGGTGTCCAC GCCCAGCCCC 
ATCCTGGAGC CCAACGGCAT CGCGGCGGGG ACGTACCAGA GGGCGCCGGA GCCGGCAGCC 
TCCGACACGT TCAAGCATCT GCAGAGGAGG AACACTCTGG AGCAGGTGGA GCAGTGGGTC 
AAAGTTCAGA AGGCTGAGCA AAGGGGCCCG CCCTCCAGAG AAAACACCCT TCCCCGCCGG 
ACGCCGCCCA CGCAGAACAA GTACACCTCC ATGGATGCCT TTCAAACTTT GCCAAAAACT 
CCACGCCACA GCCCCCCATC GGGTCGACTC GGCGAGTACA AGTACGCCCA CGACCGCCTG 
AGCCACTTCC GCCTCACCCC AGACCAGGGG GGCACGGGGT CCAACACAGT GCTGCAGCTG 
TACGAGTGGC AGCAGCGCCA ACAGTTCCGT CATGGCAGCC CCACGGCGCC AATCTACACC 
CCGGCCCCGG ATTACCCGTT CGGGCCCCGC CCCCCGTCCA CCGTGCCGCC TTCCTCCTTG 
TTGCGCAGGT CGGAGGGGTC TCCTCGCTGC GTGTCGGTGC CACCGTCGGC CGCAGACATC 
CCGCCACCGG GGCCTCCGCC AGGCACCAGC AGGACGCTGT CCCCCAACCG AAGGCCGCAC 
ACTCCTGCCG AACGGGTGAC TGTAAGGCCC GCTGGGGACG GGCCCCAGCT GAACGTCCCC 
TTCACCGTGT CCCCCCGCAG GAGCAAGTCT CAGATGCTGA AGGCTGCTAC TATTGAGAGA 
CGCTCGATGC CTTCAACTGG CTACATCACT CACACAGTCA GCGCTCCCAG CCTTCATGGC 
AAGACGCCGG AGGAGCTCAC TCTGCTCCTC ATTCAACTGC GCCGCCACCA GGCCAAGATG 
GCCGCTGTGC GTCAGCAGAC CTTAGCTCAG ATCCAGCGGC TGAACGCTCC CAGAGAGTCC 
TGCCACCAAA ACCGCCTCCT CACCGCTGTC GCCGTTAGCG ACAGCCCCCT CCTCCTCAGC 
GCCACCGCGT CTCCTGAGTC GCACCTCGAA GATGTCGGCC TCTGCAGCGT CCAGGCTGAT 
GACACGTACA TGCAGCTGAA GAAGGACCTG GAATATCTGG ACCTAAAGAT CAGAGCTCTA 
GAGCCGCTGA TCCTCGCAGT TCACAGTTTA CTACTGCACT GCACTGCTGG GCCACTCAGG 
CCTTTAATGA ACGTAGCTGG AAGTCAGGTG CTGAAGGAGG CGGGGAAGCC TGTCAAGGTT 
GCAGAAAGCG ATGTGGATGT GAAGTTGAGT CGGTTATGTG AGCAGGACAA GATTCTGAAG 
GACCTGGAGG CGAGGATCAG CTCCCTGAAG AACGACAAGG ACAAGTTGGA GAGAGTGCTT 
GACGTGTCTC ACCAGCAGAT GGAGCAGTAC CAGGAGCAGC CAGCTCATGT CCACAAGATC 
GCCTACCAGC AGAGGCTGCT GCAGGAGGAC CTGGTGACCA TCAGGGCCCA GATTTCCCGC 
GTCTCAACGG AAATGGCGCG TGCGTGGGAG GAGTACAACC GGCTGGAGCA ATCAGTGGAG 
CTCCTAAGGC TGGCACTGCA GGCACACATG GCCCACAACG ACACGTCTCA GCAGGAAAAG 
GCCGAACTGA AGCGGGAGCT GTGGAGGATT GAGGACGTCA TGGGTGGTCT TAGTGCCAGC 
AAAGCCAACT ACAAGATAAC TGTGGACTCT ATTCAGAATC CAGCAGACAG GAGATTAGTG 
CCTTCGTTGT CGGACCAGGC ATTGCCTTCC GATGGCGCGG AGGTCCAGCC TCCCCCCCGC 
AGCTCCATCT CCCACACTTT ACCTCACAGC ACCGTGCCAA AGTGGGCAGA GGATGACGCC 
CCGCCCAGGC CGCCACTGCC TCGTCTCTAT GACTACGAGG AGATGCCCCC CGTTGTGCCC 
CCGCTCCCCA AGGAGGCCTC GGTCATCCGC CACACATCGG TGCGAGGGCT CAAACGCCAG 
TCGGACGAGA GGAAGCGGGA CAGGGAGGGC GGCCAGTACA TTGCCAATGG AGACTGTAAG 
ACTGATTTGA GGTCATACCT GAGCGAACCA GAGCTGCCAG GAAGCAGCCA GCCAATCACA 
GCATCTGAAG CTGACTACCA GTATTACCCA GGCAAAGGTT TGTTGGGAAC CACGTCTCGG 
CTGGACCAGT CCAACTCCAT CTCGTCCTAC GTCACCTTGA GGAGGGCTCC CGGCAGCGCC 
GCACCAAAGG ACCGACCCAA AAGCGCCTTT GAGCGCCTGT CATCCCCCAC GGAGGGCGGC 
CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG ATGACTGCCG AGGAGCAGCT GGAGCGCATG 
AAGCGCCACC AGAGGGCGCT GGTCCGCGAG CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC 
TCTGGCGGCG GGGGCCCGGC AGCATCAGGC TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA 
CTGCCCTCCA GCCCCTGCGT GTTCGACTGG CAGCAGGAGC GACCAGGAGC TGAAGGTCAG 
AGCGACGAAG GCCTGAATCT GGTCAGAGAG AGGGAGACGA GGGACTCTAA TGAGTGGGTG 
ACGGTCACCG CCAGGCGCAT CCAGGAGGCC GACCTGGAGC CCCTCGACTA CGACCTGGAC 
ATCAGCCGGG AGCTGTCCAA ACCGGACAAA GTTGTGATCC CGGAGCGCTA CGTGGAGTCG 
GACCCAGAGG AGCCTCTGAG TCCAGAGGAG CTGGAGGAAC GCGGCCGGCG CGCGGAACGC 
ATCAAGAACC TTCTCACCAG GTCCAGTGTC CAGAACATCC AGCCATCAGC ATCGCTGGAA 
TTCAACGAGC TGGACAACGC CATGGAGGAG CAGGAGAGGA TCATGAGCGT CTCCTACGCG 
CTGGCCTCAG AGGCTTCACG CAAGAGTAAA CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409021.1
CDS	186..4028
Translation	MMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410799.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410799.2

ORF Insert Sequence:

ATGATGTCCA GATTCACGGC TGAGCAGCTC TCCAGCACCA CTGTGAGCGA GGCCTCCACC 
GCCATCACCT CCTCCACCGT GGACTCCACC TCTGCCTCCA AGGCATCTCG ATCCAGTGGG 
AAGACTCACA ACTTTGGGAA AAGAGAACAA TCCATCAAGA GGAATCCTAA CGTCCCAGTG 
GTGGTCCGAG GATGGCTGTA CAAACAGGAC AGCTCTGGGA TGCGCCTTTG GAAGAGGAAG 
TGGTTTGTCT TGTCTGATTT CTGCCTTTTC TACTACAAAG ACAGCAGAGA GGAGGCGGTC 
TTGGGCAGTA TTCCTCTGCC CAGCTATGTC ATTTCACCCG TTGGCCCCGA AGATCACATC 
AGCCGCAAGT ATGCCTTCAA GGCTAGCCAC ACCGGTATGC GTTCATACAT TTACAAGCAG 
AGCTCTGTGA TTGGCTCGCA GGCAGAGCAC ACTGGCATGC GGACGTATTA CTTCAGCGCT 
GACACCCAGG AGGACATGAG CACCTGGCTG AAGGCCATGA ACCAGGCATC GCTGATGCAG 
AACCACCCCG ACAGGCTCAG ACCCGTTGAC CAAGTCACCT CGTTGTTGCA ACAAGCTGTC 
CCGCAGACAA ACCATGTGAA TCACCACAAG ATGAAGACCG TAGACTCTGA AACCACAAAA 
GCAATTGTCC ACGAAGTTCT GCTGGAGCCC ATTCACCGTA ACACAGAAGA GCTCTGCAGC 
TTTCACCAGG ACGCCGGCAA CTTCACCCTG GAGCGCCACG CGGGGACCGC CTCTTTGGAG 
ATGGACAAAC AGAGCCCCCT TCCATCCAGA CCGCCAGCCG CCTCCACACT CCCTCTGTCG 
GACCGCGCAT CCGCCTCTGC TCCCGTGTCC AGGGTGACGT CCCGCGCTCC GTCCCGCTCC 
GCCTCGACAT TGCCCTCCAG CCTTTGCATG AGGAACGGCC TGGTGTCCAC GCCCAGCCCC 
ATCCTGGAGC CCAACGGCAT CGCGGCGGGG ACGTACCAGA GGGCGCCGGA GCCGGCAGCC 
TCCGACACGT TCAAGCATCT GCAGAGGAGG AACACTCTGG AGCAGGTGGA GCAGTGGGTC 
AAAGTTCAGA AGGCTGAGCA AAGGGGCCCG CCCTCCAGAG AAAACACCCT TCCCCGCCGG 
ACGCCGCCCA CGCAGAACAA GTACACCTCC ATGGATGCCT TTCAAACTTT GCCAAAAACT 
CCACGCCACA GCCCCCCATC GGGTCGACTC GGCGAGTACA AGTACGCCCA CGACCGCCTG 
AGCCACTTCC GCCTCACCCC AGACCAGGGG GGCACGGGGT CCAACACAGT GCTGCAGCTG 
TACGAGTGGC AGCAGCGCCA ACAGTTCCGT CATGGCAGCC CCACGGCGCC AATCTACACC 
CCGGCCCCGG ATTACCCGTT CGGGCCCCGC CCCCCGTCCA CCGTGCCGCC TTCCTCCTTG 
TTGCGCAGGT CGGAGGGGTC TCCTCGCTGC GTGTCGGTGC CACCGTCGGC CGCAGACATC 
CCGCCACCGG GGCCTCCGCC AGGCACCAGC AGGACGCTGT CCCCCAACCG AAGGCCGCAC 
ACTCCTGCCG AACGGGTGAC TGTAAGGCCC GCTGGGGACG GGCCCCAGCT GAACGTCCCC 
TTCACCGTGT CCCCCCGCAG GAGCAAGTCT CAGATGCTGA AGGCTGCTAC TATTGAGAGA 
CGCTCGATGC CTTCAACTGG CTACATCACT CACACAGTCA GCGCTCCCAG CCTTCATGGC 
AAGACGCCGG AGGAGCTCAC TCTGCTCCTC ATTCAACTGC GCCGCCACCA GGCCAAGATG 
GCCGCTGTGC GTCAGCAGAC CTTAGCTCAG ATCCAGCGGC TGAACGCTCC CAGAGAGTCC 
TGCCACCAAA ACCGCCTCCT CACCGCTGTC GCCGTTAGCG ACAGCCCCCT CCTCCTCAGC 
GCCACCGCGT CTCCTGAGTC GCACCTCGAA GATGTCGGCC TCTGCAGCGT CCAGGCTGAT 
GACACGTACA TGCAGCTGAA GAAGGACCTG GAATATCTGG ACCTAAAGAT CAGAGCTCTA 
GAGCCGCTGA TCCTCGCAGT TCACAGTTTA CTACTGCACT GCACTGCTGG GCCACTCAGG 
CCTTTAATGA ACGTAGCTGG AAGTCAGGTG CTGAAGGAGG CGGGGAAGCC TGTCAAGGTT 
GCAGAAAGCG ATGTGGATGT GAAGTTGAGT CGGTTATGTG AGCAGGACAA GATTCTGAAG 
GACCTGGAGG CGAGGATCAG CTCCCTGAAG AACGACAAGG ACAAGTTGGA GAGAGTGCTT 
GACGTGTCTC ACCAGCAGAT GGAGCAGTAC CAGGAGCAGC CAGCTCATGT CCACAAGATC 
GCCTACCAGC AGAGGCTGCT GCAGGAGGAC CTGGTGACCA TCAGGGCCCA GATTTCCCGC 
GTCTCAACGG AAATGGCGCG TGCGTGGGAG GAGTACAACC GGCTGGAGCA ATCAGTGGAG 
CTCCTAAGGC TGGCACTGCA GGCACACATG GCCCACAACG ACACGTCTCA GCAGGAAAAG 
GCCGAACTGA AGCGGGAGCT GTGGAGGATT GAGGACGTCA TGGGTGGTCT TAGTGCCAGC 
AAAGCCAACT ACAAGATAAC TGTGGACTCT ATTCAGAATC CAGCAGACAG GAGATTAGTG 
CCTTCGTTGT CGGACCAGGC ATTGCCTTCC GATGGCGCGG AGGTCCAGCC TCCCCCCCGC 
AGCTCCATCT CCCACACTTT ACCTCACAGC ACCGTGCCAA AGTGGGCAGA GGATGACGCC 
CCGCCCAGGC CGCCACTGCC TCGTCTCTAT GACTACGAGG AGATGCCCCC CGTTGTGCCC 
CCGCTCCCCA AGGAGGCCTC GGTCATCCGC CACACATCGG TGCGAGGGCT CAAACGCCAG 
TCGGACGAGA GGAAGCGGGA CAGGGAGGGC GGCCAGTACA TTGCCAATGG AGACTGTAAG 
ACTGATTTGA GGTCATACCT GAGCGAACCA GAGCTGCCAG GAAGCAGCCA GCCAATCACA 
GCATCTGAAG CTGACTACCA GTATTACCCA GGCAAAGGTT TGTTGGGAAC CACGTCTCGG 
CTGGACCAGT CCAACTCCAT CTCGTCCTAC GTCACCTTGA GGAGGGCTCC CGGCAGCGCC 
GCACCAAAGG ACCGACCCAA AAGCGCCTTT GAGCGCCTGT CATCCCCCAC GGAGGGCGGC 
CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG ATGACTGCCG AGGAGCAGCT GGAGCGCATG 
AAGCGCCACC AGAGGGCGCT GGTCCGCGAG CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC 
TCTGGCGGCG GGGGCCCGGC AGCATCAGGC TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA 
CTGCCCTCCA GCCCCTGCGT GTTCGACTGG CAGCAGGAGC GACCAGGAGC TGAAGGTCAG 
AGCGACGAAG GCCTGAATCT GGTCAGAGAG AGGGAGACGA GGGACTCTAA TGAGTGGGTG 
ACGGTCACCG CCAGGCGCAT CCAGGAGGCC GACCTGGAGC CCCTCGACTA CGACCTGGAC 
ATCAGCCGGG AGCTGTCCAA ACCGGACAAA GTTGTGATCC CGGAGCGCTA CGTGGAGTCG 
GACCCAGAGG AGCCTCTGAG TCCAGAGGAG CTGGAGGAAC GCGGCCGGCG CGCGGAACGC 
ATCAAGAACC TTCTCACCAG GTCCAGTGTC CAGAACATCC AGCCATCAGC ATCGCTGGAA 
TTCAACGAGC TGGACAACGC CATGGAGGAG CAGGAGAGGA TCATGAGCGT CTCCTACGCG 
CTGGCCTCAG AGGCTTCACG CAAGAGTAAA CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410801.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409023.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OPo196450
Clone ID Related Accession (Same CDS sequence)	XM_008410799.2 , XM_008410801.1 , XM_008410800.2
Accession Version	XM_008410801.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCA GATTCACGGC TGAGCAGCTC TCCAGCACCA CTGTGAGCGA GGCCTCCACC 
GCCATCACCT CCTCCACCGT GGACTCCACC TCTGCCTCCA AGGCATCTCG ATCCAGTGGG 
AAGACTCACA ACTTTGGGAA AAGAGAACAA TCCATCAAGA GGAATCCTAA CGTCCCAGTG 
GTGGTCCGAG GATGGCTGTA CAAACAGGAC AGCTCTGGGA TGCGCCTTTG GAAGAGGAAG 
TGGTTTGTCT TGTCTGATTT CTGCCTTTTC TACTACAAAG ACAGCAGAGA GGAGGCGGTC 
TTGGGCAGTA TTCCTCTGCC CAGCTATGTC ATTTCACCCG TTGGCCCCGA AGATCACATC 
AGCCGCAAGT ATGCCTTCAA GGCTAGCCAC ACCGGTATGC GTTCATACAT TTACAAGCAG 
AGCTCTGTGA TTGGCTCGCA GGCAGAGCAC ACTGGCATGC GGACGTATTA CTTCAGCGCT 
GACACCCAGG AGGACATGAG CACCTGGCTG AAGGCCATGA ACCAGGCATC GCTGATGCAG 
AACCACCCCG ACAGGCTCAG ACCCGTTGAC CAAGTCACCT CGTTGTTGCA ACAAGCTGTC 
CCGCAGACAA ACCATGTGAA TCACCACAAG ATGAAGACCG TAGACTCTGA AACCACAAAA 
GCAATTGTCC ACGAAGTTCT GCTGGAGCCC ATTCACCGTA ACACAGAAGA GCTCTGCAGC 
TTTCACCAGG ACGCCGGCAA CTTCACCCTG GAGCGCCACG CGGGGACCGC CTCTTTGGAG 
ATGGACAAAC AGAGCCCCCT TCCATCCAGA CCGCCAGCCG CCTCCACACT CCCTCTGTCG 
GACCGCGCAT CCGCCTCTGC TCCCGTGTCC AGGGTGACGT CCCGCGCTCC GTCCCGCTCC 
GCCTCGACAT TGCCCTCCAG CCTTTGCATG AGGAACGGCC TGGTGTCCAC GCCCAGCCCC 
ATCCTGGAGC CCAACGGCAT CGCGGCGGGG ACGTACCAGA GGGCGCCGGA GCCGGCAGCC 
TCCGACACGT TCAAGCATCT GCAGAGGAGG AACACTCTGG AGCAGGTGGA GCAGTGGGTC 
AAAGTTCAGA AGGCTGAGCA AAGGGGCCCG CCCTCCAGAG AAAACACCCT TCCCCGCCGG 
ACGCCGCCCA CGCAGAACAA GTACACCTCC ATGGATGCCT TTCAAACTTT GCCAAAAACT 
CCACGCCACA GCCCCCCATC GGGTCGACTC GGCGAGTACA AGTACGCCCA CGACCGCCTG 
AGCCACTTCC GCCTCACCCC AGACCAGGGG GGCACGGGGT CCAACACAGT GCTGCAGCTG 
TACGAGTGGC AGCAGCGCCA ACAGTTCCGT CATGGCAGCC CCACGGCGCC AATCTACACC 
CCGGCCCCGG ATTACCCGTT CGGGCCCCGC CCCCCGTCCA CCGTGCCGCC TTCCTCCTTG 
TTGCGCAGGT CGGAGGGGTC TCCTCGCTGC GTGTCGGTGC CACCGTCGGC CGCAGACATC 
CCGCCACCGG GGCCTCCGCC AGGCACCAGC AGGACGCTGT CCCCCAACCG AAGGCCGCAC 
ACTCCTGCCG AACGGGTGAC TGTAAGGCCC GCTGGGGACG GGCCCCAGCT GAACGTCCCC 
TTCACCGTGT CCCCCCGCAG GAGCAAGTCT CAGATGCTGA AGGCTGCTAC TATTGAGAGA 
CGCTCGATGC CTTCAACTGG CTACATCACT CACACAGTCA GCGCTCCCAG CCTTCATGGC 
AAGACGCCGG AGGAGCTCAC TCTGCTCCTC ATTCAACTGC GCCGCCACCA GGCCAAGATG 
GCCGCTGTGC GTCAGCAGAC CTTAGCTCAG ATCCAGCGGC TGAACGCTCC CAGAGAGTCC 
TGCCACCAAA ACCGCCTCCT CACCGCTGTC GCCGTTAGCG ACAGCCCCCT CCTCCTCAGC 
GCCACCGCGT CTCCTGAGTC GCACCTCGAA GATGTCGGCC TCTGCAGCGT CCAGGCTGAT 
GACACGTACA TGCAGCTGAA GAAGGACCTG GAATATCTGG ACCTAAAGAT CAGAGCTCTA 
GAGCCGCTGA TCCTCGCAGT TCACAGTTTA CTACTGCACT GCACTGCTGG GCCACTCAGG 
CCTTTAATGA ACGTAGCTGG AAGTCAGGTG CTGAAGGAGG CGGGGAAGCC TGTCAAGGTT 
GCAGAAAGCG ATGTGGATGT GAAGTTGAGT CGGTTATGTG AGCAGGACAA GATTCTGAAG 
GACCTGGAGG CGAGGATCAG CTCCCTGAAG AACGACAAGG ACAAGTTGGA GAGAGTGCTT 
GACGTGTCTC ACCAGCAGAT GGAGCAGTAC CAGGAGCAGC CAGCTCATGT CCACAAGATC 
GCCTACCAGC AGAGGCTGCT GCAGGAGGAC CTGGTGACCA TCAGGGCCCA GATTTCCCGC 
GTCTCAACGG AAATGGCGCG TGCGTGGGAG GAGTACAACC GGCTGGAGCA ATCAGTGGAG 
CTCCTAAGGC TGGCACTGCA GGCACACATG GCCCACAACG ACACGTCTCA GCAGGAAAAG 
GCCGAACTGA AGCGGGAGCT GTGGAGGATT GAGGACGTCA TGGGTGGTCT TAGTGCCAGC 
AAAGCCAACT ACAAGATAAC TGTGGACTCT ATTCAGAATC CAGCAGACAG GAGATTAGTG 
CCTTCGTTGT CGGACCAGGC ATTGCCTTCC GATGGCGCGG AGGTCCAGCC TCCCCCCCGC 
AGCTCCATCT CCCACACTTT ACCTCACAGC ACCGTGCCAA AGTGGGCAGA GGATGACGCC 
CCGCCCAGGC CGCCACTGCC TCGTCTCTAT GACTACGAGG AGATGCCCCC CGTTGTGCCC 
CCGCTCCCCA AGGAGGCCTC GGTCATCCGC CACACATCGG TGCGAGGGCT CAAACGCCAG 
TCGGACGAGA GGAAGCGGGA CAGGGAGGGC GGCCAGTACA TTGCCAATGG AGACTGTAAG 
ACTGATTTGA GGTCATACCT GAGCGAACCA GAGCTGCCAG GAAGCAGCCA GCCAATCACA 
GCATCTGAAG CTGACTACCA GTATTACCCA GGCAAAGGTT TGTTGGGAAC CACGTCTCGG 
CTGGACCAGT CCAACTCCAT CTCGTCCTAC GTCACCTTGA GGAGGGCTCC CGGCAGCGCC 
GCACCAAAGG ACCGACCCAA AAGCGCCTTT GAGCGCCTGT CATCCCCCAC GGAGGGCGGC 
CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG ATGACTGCCG AGGAGCAGCT GGAGCGCATG 
AAGCGCCACC AGAGGGCGCT GGTCCGCGAG CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC 
TCTGGCGGCG GGGGCCCGGC AGCATCAGGC TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA 
CTGCCCTCCA GCCCCTGCGT GTTCGACTGG CAGCAGGAGC GACCAGGAGC TGAAGGTCAG 
AGCGACGAAG GCCTGAATCT GGTCAGAGAG AGGGAGACGA GGGACTCTAA TGAGTGGGTG 
ACGGTCACCG CCAGGCGCAT CCAGGAGGCC GACCTGGAGC CCCTCGACTA CGACCTGGAC 
ATCAGCCGGG AGCTGTCCAA ACCGGACAAA GTTGTGATCC CGGAGCGCTA CGTGGAGTCG 
GACCCAGAGG AGCCTCTGAG TCCAGAGGAG CTGGAGGAAC GCGGCCGGCG CGCGGAACGC 
ATCAAGAACC TTCTCACCAG GTCCAGTGTC CAGAACATCC AGCCATCAGC ATCGCTGGAA 
TTCAACGAGC TGGACAACGC CATGGAGGAG CAGGAGAGGA TCATGAGCGT CTCCTACGCG 
CTGGCCTCAG AGGCTTCACG CAAGAGTAAA CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409023.1
CDS	182..4024
Translation	MMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410801.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410801.1

ORF Insert Sequence:

ATGATGTCCA GATTCACGGC TGAGCAGCTC TCCAGCACCA CTGTGAGCGA GGCCTCCACC 
GCCATCACCT CCTCCACCGT GGACTCCACC TCTGCCTCCA AGGCATCTCG ATCCAGTGGG 
AAGACTCACA ACTTTGGGAA AAGAGAACAA TCCATCAAGA GGAATCCTAA CGTCCCAGTG 
GTGGTCCGAG GATGGCTGTA CAAACAGGAC AGCTCTGGGA TGCGCCTTTG GAAGAGGAAG 
TGGTTTGTCT TGTCTGATTT CTGCCTTTTC TACTACAAAG ACAGCAGAGA GGAGGCGGTC 
TTGGGCAGTA TTCCTCTGCC CAGCTATGTC ATTTCACCCG TTGGCCCCGA AGATCACATC 
AGCCGCAAGT ATGCCTTCAA GGCTAGCCAC ACCGGTATGC GTTCATACAT TTACAAGCAG 
AGCTCTGTGA TTGGCTCGCA GGCAGAGCAC ACTGGCATGC GGACGTATTA CTTCAGCGCT 
GACACCCAGG AGGACATGAG CACCTGGCTG AAGGCCATGA ACCAGGCATC GCTGATGCAG 
AACCACCCCG ACAGGCTCAG ACCCGTTGAC CAAGTCACCT CGTTGTTGCA ACAAGCTGTC 
CCGCAGACAA ACCATGTGAA TCACCACAAG ATGAAGACCG TAGACTCTGA AACCACAAAA 
GCAATTGTCC ACGAAGTTCT GCTGGAGCCC ATTCACCGTA ACACAGAAGA GCTCTGCAGC 
TTTCACCAGG ACGCCGGCAA CTTCACCCTG GAGCGCCACG CGGGGACCGC CTCTTTGGAG 
ATGGACAAAC AGAGCCCCCT TCCATCCAGA CCGCCAGCCG CCTCCACACT CCCTCTGTCG 
GACCGCGCAT CCGCCTCTGC TCCCGTGTCC AGGGTGACGT CCCGCGCTCC GTCCCGCTCC 
GCCTCGACAT TGCCCTCCAG CCTTTGCATG AGGAACGGCC TGGTGTCCAC GCCCAGCCCC 
ATCCTGGAGC CCAACGGCAT CGCGGCGGGG ACGTACCAGA GGGCGCCGGA GCCGGCAGCC 
TCCGACACGT TCAAGCATCT GCAGAGGAGG AACACTCTGG AGCAGGTGGA GCAGTGGGTC 
AAAGTTCAGA AGGCTGAGCA AAGGGGCCCG CCCTCCAGAG AAAACACCCT TCCCCGCCGG 
ACGCCGCCCA CGCAGAACAA GTACACCTCC ATGGATGCCT TTCAAACTTT GCCAAAAACT 
CCACGCCACA GCCCCCCATC GGGTCGACTC GGCGAGTACA AGTACGCCCA CGACCGCCTG 
AGCCACTTCC GCCTCACCCC AGACCAGGGG GGCACGGGGT CCAACACAGT GCTGCAGCTG 
TACGAGTGGC AGCAGCGCCA ACAGTTCCGT CATGGCAGCC CCACGGCGCC AATCTACACC 
CCGGCCCCGG ATTACCCGTT CGGGCCCCGC CCCCCGTCCA CCGTGCCGCC TTCCTCCTTG 
TTGCGCAGGT CGGAGGGGTC TCCTCGCTGC GTGTCGGTGC CACCGTCGGC CGCAGACATC 
CCGCCACCGG GGCCTCCGCC AGGCACCAGC AGGACGCTGT CCCCCAACCG AAGGCCGCAC 
ACTCCTGCCG AACGGGTGAC TGTAAGGCCC GCTGGGGACG GGCCCCAGCT GAACGTCCCC 
TTCACCGTGT CCCCCCGCAG GAGCAAGTCT CAGATGCTGA AGGCTGCTAC TATTGAGAGA 
CGCTCGATGC CTTCAACTGG CTACATCACT CACACAGTCA GCGCTCCCAG CCTTCATGGC 
AAGACGCCGG AGGAGCTCAC TCTGCTCCTC ATTCAACTGC GCCGCCACCA GGCCAAGATG 
GCCGCTGTGC GTCAGCAGAC CTTAGCTCAG ATCCAGCGGC TGAACGCTCC CAGAGAGTCC 
TGCCACCAAA ACCGCCTCCT CACCGCTGTC GCCGTTAGCG ACAGCCCCCT CCTCCTCAGC 
GCCACCGCGT CTCCTGAGTC GCACCTCGAA GATGTCGGCC TCTGCAGCGT CCAGGCTGAT 
GACACGTACA TGCAGCTGAA GAAGGACCTG GAATATCTGG ACCTAAAGAT CAGAGCTCTA 
GAGCCGCTGA TCCTCGCAGT TCACAGTTTA CTACTGCACT GCACTGCTGG GCCACTCAGG 
CCTTTAATGA ACGTAGCTGG AAGTCAGGTG CTGAAGGAGG CGGGGAAGCC TGTCAAGGTT 
GCAGAAAGCG ATGTGGATGT GAAGTTGAGT CGGTTATGTG AGCAGGACAA GATTCTGAAG 
GACCTGGAGG CGAGGATCAG CTCCCTGAAG AACGACAAGG ACAAGTTGGA GAGAGTGCTT 
GACGTGTCTC ACCAGCAGAT GGAGCAGTAC CAGGAGCAGC CAGCTCATGT CCACAAGATC 
GCCTACCAGC AGAGGCTGCT GCAGGAGGAC CTGGTGACCA TCAGGGCCCA GATTTCCCGC 
GTCTCAACGG AAATGGCGCG TGCGTGGGAG GAGTACAACC GGCTGGAGCA ATCAGTGGAG 
CTCCTAAGGC TGGCACTGCA GGCACACATG GCCCACAACG ACACGTCTCA GCAGGAAAAG 
GCCGAACTGA AGCGGGAGCT GTGGAGGATT GAGGACGTCA TGGGTGGTCT TAGTGCCAGC 
AAAGCCAACT ACAAGATAAC TGTGGACTCT ATTCAGAATC CAGCAGACAG GAGATTAGTG 
CCTTCGTTGT CGGACCAGGC ATTGCCTTCC GATGGCGCGG AGGTCCAGCC TCCCCCCCGC 
AGCTCCATCT CCCACACTTT ACCTCACAGC ACCGTGCCAA AGTGGGCAGA GGATGACGCC 
CCGCCCAGGC CGCCACTGCC TCGTCTCTAT GACTACGAGG AGATGCCCCC CGTTGTGCCC 
CCGCTCCCCA AGGAGGCCTC GGTCATCCGC CACACATCGG TGCGAGGGCT CAAACGCCAG 
TCGGACGAGA GGAAGCGGGA CAGGGAGGGC GGCCAGTACA TTGCCAATGG AGACTGTAAG 
ACTGATTTGA GGTCATACCT GAGCGAACCA GAGCTGCCAG GAAGCAGCCA GCCAATCACA 
GCATCTGAAG CTGACTACCA GTATTACCCA GGCAAAGGTT TGTTGGGAAC CACGTCTCGG 
CTGGACCAGT CCAACTCCAT CTCGTCCTAC GTCACCTTGA GGAGGGCTCC CGGCAGCGCC 
GCACCAAAGG ACCGACCCAA AAGCGCCTTT GAGCGCCTGT CATCCCCCAC GGAGGGCGGC 
CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG ATGACTGCCG AGGAGCAGCT GGAGCGCATG 
AAGCGCCACC AGAGGGCGCT GGTCCGCGAG CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC 
TCTGGCGGCG GGGGCCCGGC AGCATCAGGC TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA 
CTGCCCTCCA GCCCCTGCGT GTTCGACTGG CAGCAGGAGC GACCAGGAGC TGAAGGTCAG 
AGCGACGAAG GCCTGAATCT GGTCAGAGAG AGGGAGACGA GGGACTCTAA TGAGTGGGTG 
ACGGTCACCG CCAGGCGCAT CCAGGAGGCC GACCTGGAGC CCCTCGACTA CGACCTGGAC 
ATCAGCCGGG AGCTGTCCAA ACCGGACAAA GTTGTGATCC CGGAGCGCTA CGTGGAGTCG 
GACCCAGAGG AGCCTCTGAG TCCAGAGGAG CTGGAGGAAC GCGGCCGGCG CGCGGAACGC 
ATCAAGAACC TTCTCACCAG GTCCAGTGTC CAGAACATCC AGCCATCAGC ATCGCTGGAA 
TTCAACGAGC TGGACAACGC CATGGAGGAG CAGGAGAGGA TCATGAGCGT CTCCTACGCG 
CTGGCCTCAG AGGCTTCACG CAAGAGTAAA CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410800.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409022.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X12, mRNA.

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CloneID	OPo196450
Clone ID Related Accession (Same CDS sequence)	XM_008410799.2 , XM_008410801.1 , XM_008410800.2
Accession Version	XM_008410800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCCA GATTCACGGC TGAGCAGCTC TCCAGCACCA CTGTGAGCGA GGCCTCCACC 
GCCATCACCT CCTCCACCGT GGACTCCACC TCTGCCTCCA AGGCATCTCG ATCCAGTGGG 
AAGACTCACA ACTTTGGGAA AAGAGAACAA TCCATCAAGA GGAATCCTAA CGTCCCAGTG 
GTGGTCCGAG GATGGCTGTA CAAACAGGAC AGCTCTGGGA TGCGCCTTTG GAAGAGGAAG 
TGGTTTGTCT TGTCTGATTT CTGCCTTTTC TACTACAAAG ACAGCAGAGA GGAGGCGGTC 
TTGGGCAGTA TTCCTCTGCC CAGCTATGTC ATTTCACCCG TTGGCCCCGA AGATCACATC 
AGCCGCAAGT ATGCCTTCAA GGCTAGCCAC ACCGGTATGC GTTCATACAT TTACAAGCAG 
AGCTCTGTGA TTGGCTCGCA GGCAGAGCAC ACTGGCATGC GGACGTATTA CTTCAGCGCT 
GACACCCAGG AGGACATGAG CACCTGGCTG AAGGCCATGA ACCAGGCATC GCTGATGCAG 
AACCACCCCG ACAGGCTCAG ACCCGTTGAC CAAGTCACCT CGTTGTTGCA ACAAGCTGTC 
CCGCAGACAA ACCATGTGAA TCACCACAAG ATGAAGACCG TAGACTCTGA AACCACAAAA 
GCAATTGTCC ACGAAGTTCT GCTGGAGCCC ATTCACCGTA ACACAGAAGA GCTCTGCAGC 
TTTCACCAGG ACGCCGGCAA CTTCACCCTG GAGCGCCACG CGGGGACCGC CTCTTTGGAG 
ATGGACAAAC AGAGCCCCCT TCCATCCAGA CCGCCAGCCG CCTCCACACT CCCTCTGTCG 
GACCGCGCAT CCGCCTCTGC TCCCGTGTCC AGGGTGACGT CCCGCGCTCC GTCCCGCTCC 
GCCTCGACAT TGCCCTCCAG CCTTTGCATG AGGAACGGCC TGGTGTCCAC GCCCAGCCCC 
ATCCTGGAGC CCAACGGCAT CGCGGCGGGG ACGTACCAGA GGGCGCCGGA GCCGGCAGCC 
TCCGACACGT TCAAGCATCT GCAGAGGAGG AACACTCTGG AGCAGGTGGA GCAGTGGGTC 
AAAGTTCAGA AGGCTGAGCA AAGGGGCCCG CCCTCCAGAG AAAACACCCT TCCCCGCCGG 
ACGCCGCCCA CGCAGAACAA GTACACCTCC ATGGATGCCT TTCAAACTTT GCCAAAAACT 
CCACGCCACA GCCCCCCATC GGGTCGACTC GGCGAGTACA AGTACGCCCA CGACCGCCTG 
AGCCACTTCC GCCTCACCCC AGACCAGGGG GGCACGGGGT CCAACACAGT GCTGCAGCTG 
TACGAGTGGC AGCAGCGCCA ACAGTTCCGT CATGGCAGCC CCACGGCGCC AATCTACACC 
CCGGCCCCGG ATTACCCGTT CGGGCCCCGC CCCCCGTCCA CCGTGCCGCC TTCCTCCTTG 
TTGCGCAGGT CGGAGGGGTC TCCTCGCTGC GTGTCGGTGC CACCGTCGGC CGCAGACATC 
CCGCCACCGG GGCCTCCGCC AGGCACCAGC AGGACGCTGT CCCCCAACCG AAGGCCGCAC 
ACTCCTGCCG AACGGGTGAC TGTAAGGCCC GCTGGGGACG GGCCCCAGCT GAACGTCCCC 
TTCACCGTGT CCCCCCGCAG GAGCAAGTCT CAGATGCTGA AGGCTGCTAC TATTGAGAGA 
CGCTCGATGC CTTCAACTGG CTACATCACT CACACAGTCA GCGCTCCCAG CCTTCATGGC 
AAGACGCCGG AGGAGCTCAC TCTGCTCCTC ATTCAACTGC GCCGCCACCA GGCCAAGATG 
GCCGCTGTGC GTCAGCAGAC CTTAGCTCAG ATCCAGCGGC TGAACGCTCC CAGAGAGTCC 
TGCCACCAAA ACCGCCTCCT CACCGCTGTC GCCGTTAGCG ACAGCCCCCT CCTCCTCAGC 
GCCACCGCGT CTCCTGAGTC GCACCTCGAA GATGTCGGCC TCTGCAGCGT CCAGGCTGAT 
GACACGTACA TGCAGCTGAA GAAGGACCTG GAATATCTGG ACCTAAAGAT CAGAGCTCTA 
GAGCCGCTGA TCCTCGCAGT TCACAGTTTA CTACTGCACT GCACTGCTGG GCCACTCAGG 
CCTTTAATGA ACGTAGCTGG AAGTCAGGTG CTGAAGGAGG CGGGGAAGCC TGTCAAGGTT 
GCAGAAAGCG ATGTGGATGT GAAGTTGAGT CGGTTATGTG AGCAGGACAA GATTCTGAAG 
GACCTGGAGG CGAGGATCAG CTCCCTGAAG AACGACAAGG ACAAGTTGGA GAGAGTGCTT 
GACGTGTCTC ACCAGCAGAT GGAGCAGTAC CAGGAGCAGC CAGCTCATGT CCACAAGATC 
GCCTACCAGC AGAGGCTGCT GCAGGAGGAC CTGGTGACCA TCAGGGCCCA GATTTCCCGC 
GTCTCAACGG AAATGGCGCG TGCGTGGGAG GAGTACAACC GGCTGGAGCA ATCAGTGGAG 
CTCCTAAGGC TGGCACTGCA GGCACACATG GCCCACAACG ACACGTCTCA GCAGGAAAAG 
GCCGAACTGA AGCGGGAGCT GTGGAGGATT GAGGACGTCA TGGGTGGTCT TAGTGCCAGC 
AAAGCCAACT ACAAGATAAC TGTGGACTCT ATTCAGAATC CAGCAGACAG GAGATTAGTG 
CCTTCGTTGT CGGACCAGGC ATTGCCTTCC GATGGCGCGG AGGTCCAGCC TCCCCCCCGC 
AGCTCCATCT CCCACACTTT ACCTCACAGC ACCGTGCCAA AGTGGGCAGA GGATGACGCC 
CCGCCCAGGC CGCCACTGCC TCGTCTCTAT GACTACGAGG AGATGCCCCC CGTTGTGCCC 
CCGCTCCCCA AGGAGGCCTC GGTCATCCGC CACACATCGG TGCGAGGGCT CAAACGCCAG 
TCGGACGAGA GGAAGCGGGA CAGGGAGGGC GGCCAGTACA TTGCCAATGG AGACTGTAAG 
ACTGATTTGA GGTCATACCT GAGCGAACCA GAGCTGCCAG GAAGCAGCCA GCCAATCACA 
GCATCTGAAG CTGACTACCA GTATTACCCA GGCAAAGGTT TGTTGGGAAC CACGTCTCGG 
CTGGACCAGT CCAACTCCAT CTCGTCCTAC GTCACCTTGA GGAGGGCTCC CGGCAGCGCC 
GCACCAAAGG ACCGACCCAA AAGCGCCTTT GAGCGCCTGT CATCCCCCAC GGAGGGCGGC 
CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG ATGACTGCCG AGGAGCAGCT GGAGCGCATG 
AAGCGCCACC AGAGGGCGCT GGTCCGCGAG CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC 
TCTGGCGGCG GGGGCCCGGC AGCATCAGGC TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA 
CTGCCCTCCA GCCCCTGCGT GTTCGACTGG CAGCAGGAGC GACCAGGAGC TGAAGGTCAG 
AGCGACGAAG GCCTGAATCT GGTCAGAGAG AGGGAGACGA GGGACTCTAA TGAGTGGGTG 
ACGGTCACCG CCAGGCGCAT CCAGGAGGCC GACCTGGAGC CCCTCGACTA CGACCTGGAC 
ATCAGCCGGG AGCTGTCCAA ACCGGACAAA GTTGTGATCC CGGAGCGCTA CGTGGAGTCG 
GACCCAGAGG AGCCTCTGAG TCCAGAGGAG CTGGAGGAAC GCGGCCGGCG CGCGGAACGC 
ATCAAGAACC TTCTCACCAG GTCCAGTGTC CAGAACATCC AGCCATCAGC ATCGCTGGAA 
TTCAACGAGC TGGACAACGC CATGGAGGAG CAGGAGAGGA TCATGAGCGT CTCCTACGCG 
CTGGCCTCAG AGGCTTCACG CAAGAGTAAA CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409022.1
CDS	197..4039
Translation	MMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410800.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410800.2

ORF Insert Sequence:

ATGATGTCCA GATTCACGGC TGAGCAGCTC TCCAGCACCA CTGTGAGCGA GGCCTCCACC 
GCCATCACCT CCTCCACCGT GGACTCCACC TCTGCCTCCA AGGCATCTCG ATCCAGTGGG 
AAGACTCACA ACTTTGGGAA AAGAGAACAA TCCATCAAGA GGAATCCTAA CGTCCCAGTG 
GTGGTCCGAG GATGGCTGTA CAAACAGGAC AGCTCTGGGA TGCGCCTTTG GAAGAGGAAG 
TGGTTTGTCT TGTCTGATTT CTGCCTTTTC TACTACAAAG ACAGCAGAGA GGAGGCGGTC 
TTGGGCAGTA TTCCTCTGCC CAGCTATGTC ATTTCACCCG TTGGCCCCGA AGATCACATC 
AGCCGCAAGT ATGCCTTCAA GGCTAGCCAC ACCGGTATGC GTTCATACAT TTACAAGCAG 
AGCTCTGTGA TTGGCTCGCA GGCAGAGCAC ACTGGCATGC GGACGTATTA CTTCAGCGCT 
GACACCCAGG AGGACATGAG CACCTGGCTG AAGGCCATGA ACCAGGCATC GCTGATGCAG 
AACCACCCCG ACAGGCTCAG ACCCGTTGAC CAAGTCACCT CGTTGTTGCA ACAAGCTGTC 
CCGCAGACAA ACCATGTGAA TCACCACAAG ATGAAGACCG TAGACTCTGA AACCACAAAA 
GCAATTGTCC ACGAAGTTCT GCTGGAGCCC ATTCACCGTA ACACAGAAGA GCTCTGCAGC 
TTTCACCAGG ACGCCGGCAA CTTCACCCTG GAGCGCCACG CGGGGACCGC CTCTTTGGAG 
ATGGACAAAC AGAGCCCCCT TCCATCCAGA CCGCCAGCCG CCTCCACACT CCCTCTGTCG 
GACCGCGCAT CCGCCTCTGC TCCCGTGTCC AGGGTGACGT CCCGCGCTCC GTCCCGCTCC 
GCCTCGACAT TGCCCTCCAG CCTTTGCATG AGGAACGGCC TGGTGTCCAC GCCCAGCCCC 
ATCCTGGAGC CCAACGGCAT CGCGGCGGGG ACGTACCAGA GGGCGCCGGA GCCGGCAGCC 
TCCGACACGT TCAAGCATCT GCAGAGGAGG AACACTCTGG AGCAGGTGGA GCAGTGGGTC 
AAAGTTCAGA AGGCTGAGCA AAGGGGCCCG CCCTCCAGAG AAAACACCCT TCCCCGCCGG 
ACGCCGCCCA CGCAGAACAA GTACACCTCC ATGGATGCCT TTCAAACTTT GCCAAAAACT 
CCACGCCACA GCCCCCCATC GGGTCGACTC GGCGAGTACA AGTACGCCCA CGACCGCCTG 
AGCCACTTCC GCCTCACCCC AGACCAGGGG GGCACGGGGT CCAACACAGT GCTGCAGCTG 
TACGAGTGGC AGCAGCGCCA ACAGTTCCGT CATGGCAGCC CCACGGCGCC AATCTACACC 
CCGGCCCCGG ATTACCCGTT CGGGCCCCGC CCCCCGTCCA CCGTGCCGCC TTCCTCCTTG 
TTGCGCAGGT CGGAGGGGTC TCCTCGCTGC GTGTCGGTGC CACCGTCGGC CGCAGACATC 
CCGCCACCGG GGCCTCCGCC AGGCACCAGC AGGACGCTGT CCCCCAACCG AAGGCCGCAC 
ACTCCTGCCG AACGGGTGAC TGTAAGGCCC GCTGGGGACG GGCCCCAGCT GAACGTCCCC 
TTCACCGTGT CCCCCCGCAG GAGCAAGTCT CAGATGCTGA AGGCTGCTAC TATTGAGAGA 
CGCTCGATGC CTTCAACTGG CTACATCACT CACACAGTCA GCGCTCCCAG CCTTCATGGC 
AAGACGCCGG AGGAGCTCAC TCTGCTCCTC ATTCAACTGC GCCGCCACCA GGCCAAGATG 
GCCGCTGTGC GTCAGCAGAC CTTAGCTCAG ATCCAGCGGC TGAACGCTCC CAGAGAGTCC 
TGCCACCAAA ACCGCCTCCT CACCGCTGTC GCCGTTAGCG ACAGCCCCCT CCTCCTCAGC 
GCCACCGCGT CTCCTGAGTC GCACCTCGAA GATGTCGGCC TCTGCAGCGT CCAGGCTGAT 
GACACGTACA TGCAGCTGAA GAAGGACCTG GAATATCTGG ACCTAAAGAT CAGAGCTCTA 
GAGCCGCTGA TCCTCGCAGT TCACAGTTTA CTACTGCACT GCACTGCTGG GCCACTCAGG 
CCTTTAATGA ACGTAGCTGG AAGTCAGGTG CTGAAGGAGG CGGGGAAGCC TGTCAAGGTT 
GCAGAAAGCG ATGTGGATGT GAAGTTGAGT CGGTTATGTG AGCAGGACAA GATTCTGAAG 
GACCTGGAGG CGAGGATCAG CTCCCTGAAG AACGACAAGG ACAAGTTGGA GAGAGTGCTT 
GACGTGTCTC ACCAGCAGAT GGAGCAGTAC CAGGAGCAGC CAGCTCATGT CCACAAGATC 
GCCTACCAGC AGAGGCTGCT GCAGGAGGAC CTGGTGACCA TCAGGGCCCA GATTTCCCGC 
GTCTCAACGG AAATGGCGCG TGCGTGGGAG GAGTACAACC GGCTGGAGCA ATCAGTGGAG 
CTCCTAAGGC TGGCACTGCA GGCACACATG GCCCACAACG ACACGTCTCA GCAGGAAAAG 
GCCGAACTGA AGCGGGAGCT GTGGAGGATT GAGGACGTCA TGGGTGGTCT TAGTGCCAGC 
AAAGCCAACT ACAAGATAAC TGTGGACTCT ATTCAGAATC CAGCAGACAG GAGATTAGTG 
CCTTCGTTGT CGGACCAGGC ATTGCCTTCC GATGGCGCGG AGGTCCAGCC TCCCCCCCGC 
AGCTCCATCT CCCACACTTT ACCTCACAGC ACCGTGCCAA AGTGGGCAGA GGATGACGCC 
CCGCCCAGGC CGCCACTGCC TCGTCTCTAT GACTACGAGG AGATGCCCCC CGTTGTGCCC 
CCGCTCCCCA AGGAGGCCTC GGTCATCCGC CACACATCGG TGCGAGGGCT CAAACGCCAG 
TCGGACGAGA GGAAGCGGGA CAGGGAGGGC GGCCAGTACA TTGCCAATGG AGACTGTAAG 
ACTGATTTGA GGTCATACCT GAGCGAACCA GAGCTGCCAG GAAGCAGCCA GCCAATCACA 
GCATCTGAAG CTGACTACCA GTATTACCCA GGCAAAGGTT TGTTGGGAAC CACGTCTCGG 
CTGGACCAGT CCAACTCCAT CTCGTCCTAC GTCACCTTGA GGAGGGCTCC CGGCAGCGCC 
GCACCAAAGG ACCGACCCAA AAGCGCCTTT GAGCGCCTGT CATCCCCCAC GGAGGGCGGC 
CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG ATGACTGCCG AGGAGCAGCT GGAGCGCATG 
AAGCGCCACC AGAGGGCGCT GGTCCGCGAG CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC 
TCTGGCGGCG GGGGCCCGGC AGCATCAGGC TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA 
CTGCCCTCCA GCCCCTGCGT GTTCGACTGG CAGCAGGAGC GACCAGGAGC TGAAGGTCAG 
AGCGACGAAG GCCTGAATCT GGTCAGAGAG AGGGAGACGA GGGACTCTAA TGAGTGGGTG 
ACGGTCACCG CCAGGCGCAT CCAGGAGGCC GACCTGGAGC CCCTCGACTA CGACCTGGAC 
ATCAGCCGGG AGCTGTCCAA ACCGGACAAA GTTGTGATCC CGGAGCGCTA CGTGGAGTCG 
GACCCAGAGG AGCCTCTGAG TCCAGAGGAG CTGGAGGAAC GCGGCCGGCG CGCGGAACGC 
ATCAAGAACC TTCTCACCAG GTCCAGTGTC CAGAACATCC AGCCATCAGC ATCGCTGGAA 
TTCAACGAGC TGGACAACGC CATGGAGGAG CAGGAGAGGA TCATGAGCGT CTCCTACGCG 
CTGGCCTCAG AGGCTTCACG CAAGAGTAAA CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_017305317.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017160806.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OPo196451
Clone ID Related Accession (Same CDS sequence)	XM_017305317.1
Accession Version	XM_017305317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGGCTGAT GACACGTACA TGCAGCTGAA GAAGGACCTG 
GAATATCTGG ACCTAAAGGT GAAGTTGAGT CGGTTATGTG AGCAGGACAA GATTCTGAAG 
GACCTGGAGG CGAGGATCAG CTCCCTGAAG AACGACAAGG ACAAGTTGGA GAGAGTGCTT 
GACGTGTCTC ACCAGCAGAT GGAGCAGTAC CAGGAGCAGC CAGCTCATGT CCACAAGATC 
GCCTACCAGC AGAGGCTGCT GCAGGAGGAC CTGGTGACCA TCAGGGCCCA GATTTCCCGC 
GTCTCAACGG AAATGGCGCG TGCGTGGGAG GAGTACAACC GGCTGGAGCA ATCAGTGGAG 
CTCCTAAGGC TGGCACTGCA GGCACACATG GCCCACAACG ACACGTCTCA GCAGGAAAAG 
GCCGAACTGA AGCGGGAGCT GTGGAGGATT GAGGACGTCA TGGGTGGTCT TAGTGCCAGC 
AAAGCCAACT ACAAGATAAC TGTGGACTCT ATTCAGAATC CAGCAGACAG GAGATTAGTG 
CCTTCGTTGT CGGACCAGGC ATTGCCTTCC GATGGCGCGG AGGTCCAGCC TCCCCCCCGC 
AGCTCCATCT CCCACACTTT ACCTCACAGC ACCGTGCCAA AGTGGGCAGA GGATGACGCC 
CCGCCCAGGC CGCCACTGCC TCGTCTCTAT GACTACGAGG AGATGCCCCC CGTTGTGCCC 
CCGCTCCCCA AGGAGGCCTC GGTCATCCGC CACACATCGG TGCGAGGGCT CAAACGCCAG 
TCGGACGAGA GGAAGCGGGA CAGGGAGGGC GGCCAGTACA TTGCCAATGG AGACTGTAAG 
ACTGATTTGA GGTCATACCT GAGCGAACCA GAGCTGCCAG GAAGCAGCCA GCCAATCACA 
GCATCTGAAG CTGACTACCA GTATTACCCA GGCAAAGGTT TGTTGGGAAC CACGTCTCGG 
CTGGACCAGT CCAACTCCAT CTCGTCCTAC GTCACCTTGA GGAGGGCTCC CGGCAGCGCC 
GCACCAAAGG ACCGACCCAA AAGCGCCTTT GAGCGCCTGT CATCCCCCAC GGAGGGCGGC 
CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG ATGACTGCCG AGGAGCAGCT GGAGCGCATG 
AAGCGCCACC AGAGGGCGCT GGTCCGCGAG CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC 
TCTGGCGGCG GGGGCCCGGC AGCATCAGGC TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA 
CTGCCCTCCA GCCCCTGCGT GTTCGACTGG CAGCAGGAGC GACCAGGAGC TGAAGGTCAG 
AGCGACGAAG GCCTGAATCT GGTCAGAGAG AGGGAGACGA GGGACTCTAA TGAGTGGGTG 
ACGGTCACCG CCAGGCGCAT CCAGGAGGCC GACCTGGAGC CCCTCGACTA CGACCTGGAC 
ATCAGCCGGG AGCTGTCCAA ACCGGACAAA GTTGTGATCC CGGAGCGCTA CGTGGAGTCG 
GACCCAGAGG AGCCTCTGAG TCCAGAGGAG CTGGAGGAAC GCGGCCGGCG CGCGGAACGC 
ATCAAGAACC TTCTCACCAG GTCCAGTGTC CAGAACATCC AGCCATCAGC ATCGCTGGAA 
TTCAACGAGC TGGACAACGC CATGGAGGAG CAGGAGAGGA TCATGAGCGT CTCCTACGCG 
CTGGCCTCAG AGGCTTCACG CAAGAGTAAA CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017160806.1
CDS	166..3948
Translation	MAAPLGRDTLPEHWSYGVCRDGRVFFINDETRNTTWLHPRSSEPVNSGHMIRSDLPPGWEEGFTDEGASYFINHNQKSTSFCHPVTGQISSENVDFLLQEQSQGPRMMSRFTAEQLSSTTVSEASTAITSSTVDSTSASKASRSSGKTHNFGKREQSIKRNPNVPVVVRGWLYKQDSSGMRLWKRKWFVLSDFCLFYYKDSREEAVLGSIPLPSYVISPVGPEDHISRKYAFKASHTGMRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTADDTYMQLKKDLEYLDLKVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_017305317.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017305317.1

ORF Insert Sequence:

ATGGCGGCGC CTCTCGGACG GGACACCTTA CCCGAACACT GGTCCTACGG TGTCTGCAGG 
GACGGAAGGG TTTTCTTCAT CAACGATGAG ACCCGGAACA CCACCTGGCT CCATCCCCGC 
TCCAGTGAAC CTGTAAACTC CGGACACATG ATCCGCTCTG ACTTGCCACC GGGATGGGAG 
GAAGGCTTTA CGGACGAAGG AGCCAGTTAC TTCATAAATC ATAACCAAAA GAGCACGTCG 
TTCTGTCATC CTGTAACTGG ACAGATTTCC TCAGAGAACG TGGACTTCCT CCTGCAGGAA 
CAGTCGCAAG GTCCTCGCAT GATGTCCAGA TTCACGGCTG AGCAGCTCTC CAGCACCACT 
GTGAGCGAGG CCTCCACCGC CATCACCTCC TCCACCGTGG ACTCCACCTC TGCCTCCAAG 
GCATCTCGAT CCAGTGGGAA GACTCACAAC TTTGGGAAAA GAGAACAATC CATCAAGAGG 
AATCCTAACG TCCCAGTGGT GGTCCGAGGA TGGCTGTACA AACAGGACAG CTCTGGGATG 
CGCCTTTGGA AGAGGAAGTG GTTTGTCTTG TCTGATTTCT GCCTTTTCTA CTACAAAGAC 
AGCAGAGAGG AGGCGGTCTT GGGCAGTATT CCTCTGCCCA GCTATGTCAT TTCACCCGTT 
GGCCCCGAAG ATCACATCAG CCGCAAGTAT GCCTTCAAGG CTAGCCACAC CGGTATGCGT 
TCATACATTT ACAAGCAGAG CTCTGTGATT GGCTCGCAGG CAGAGCACAC TGGCATGCGG 
ACGTATTACT TCAGCGCTGA CACCCAGGAG GACATGAGCA CCTGGCTGAA GGCCATGAAC 
CAGGCATCGC TGATGCAGAA CCACCCCGAC AGGCTCAGAC CCGTTGACCA AGTCACCTCG 
TTGTTGCAAC AAGCTGTCCC GCAGACAAAC CATGTGAATC ACCACAAGAT GAAGACCGTA 
GACTCTGAAA CCACAAAAGC AATTGTCCAC GAAGTTCTGC TGGAGCCCAT TCACCGTAAC 
ACAGAAGAGC TCTGCAGCTT TCACCAGGAC GCCGGCAACT TCACCCTGGA GCGCCACGCG 
GGGACCGCCT CTTTGGAGAT GGACAAACAG AGCCCCCTTC CATCCAGACC GCCAGCCGCC 
TCCACACTCC CTCTGTCGGA CCGCGCATCC GCCTCTGCTC CCGTGTCCAG GGTGACGTCC 
CGCGCTCCGT CCCGCTCCGC CTCGACATTG CCCTCCAGCC TTTGCATGAG GAACGGCCTG 
GTGTCCACGC CCAGCCCCAT CCTGGAGCCC AACGGCATCG CGGCGGGGAC GTACCAGAGG 
GCGCCGGAGC CGGCAGCCTC CGACACGTTC AAGCATCTGC AGAGGAGGAA CACTCTGGAG 
CAGGTGGAGC AGTGGGTCAA AGTTCAGAAG GCTGAGCAAA GGGGCCCGCC CTCCAGAGAA 
AACACCCTTC CCCGCCGGAC GCCGCCCACG CAGAACAAGT ACACCTCCAT GGATGCCTTT 
CAAACTTTGC CAAAAACTCC ACGCCACAGC CCCCCATCGG GTCGACTCGG CGAGTACAAG 
TACGCCCACG ACCGCCTGAG CCACTTCCGC CTCACCCCAG ACCAGGGGGG CACGGGGTCC 
AACACAGTGC TGCAGCTGTA CGAGTGGCAG CAGCGCCAAC AGTTCCGTCA TGGCAGCCCC 
ACGGCGCCAA TCTACACCCC GGCCCCGGAT TACCCGTTCG GGCCCCGCCC CCCGTCCACC 
GTGCCGCCTT CCTCCTTGTT GCGCAGGTCG GAGGGGTCTC CTCGCTGCGT GTCGGTGCCA 
CCGTCGGCCG CAGACATCCC GCCACCGGGG CCTCCGCCAG GCACCAGCAG GACGCTGTCC 
CCCAACCGAA GGCCGCACAC TCCTGCCGAA CGGGTGACTG TAAGGCCCGC TGGGGACGGG 
CCCCAGCTGA ACGTCCCCTT CACCGTGTCC CCCCGCAGGA GCAAGTCTCA GATGCTGAAG 
GCTGCTACTA TTGAGAGACG CTCGATGCCT TCAACTGGCT ACATCACTCA CACAGTCAGC 
GCTCCCAGCC TTCATGGCAA GACGGCTGAT GACACGTACA TGCAGCTGAA GAAGGACCTG 
GAATATCTGG ACCTAAAGGT GAAGTTGAGT CGGTTATGTG AGCAGGACAA GATTCTGAAG 
GACCTGGAGG CGAGGATCAG CTCCCTGAAG AACGACAAGG ACAAGTTGGA GAGAGTGCTT 
GACGTGTCTC ACCAGCAGAT GGAGCAGTAC CAGGAGCAGC CAGCTCATGT CCACAAGATC 
GCCTACCAGC AGAGGCTGCT GCAGGAGGAC CTGGTGACCA TCAGGGCCCA GATTTCCCGC 
GTCTCAACGG AAATGGCGCG TGCGTGGGAG GAGTACAACC GGCTGGAGCA ATCAGTGGAG 
CTCCTAAGGC TGGCACTGCA GGCACACATG GCCCACAACG ACACGTCTCA GCAGGAAAAG 
GCCGAACTGA AGCGGGAGCT GTGGAGGATT GAGGACGTCA TGGGTGGTCT TAGTGCCAGC 
AAAGCCAACT ACAAGATAAC TGTGGACTCT ATTCAGAATC CAGCAGACAG GAGATTAGTG 
CCTTCGTTGT CGGACCAGGC ATTGCCTTCC GATGGCGCGG AGGTCCAGCC TCCCCCCCGC 
AGCTCCATCT CCCACACTTT ACCTCACAGC ACCGTGCCAA AGTGGGCAGA GGATGACGCC 
CCGCCCAGGC CGCCACTGCC TCGTCTCTAT GACTACGAGG AGATGCCCCC CGTTGTGCCC 
CCGCTCCCCA AGGAGGCCTC GGTCATCCGC CACACATCGG TGCGAGGGCT CAAACGCCAG 
TCGGACGAGA GGAAGCGGGA CAGGGAGGGC GGCCAGTACA TTGCCAATGG AGACTGTAAG 
ACTGATTTGA GGTCATACCT GAGCGAACCA GAGCTGCCAG GAAGCAGCCA GCCAATCACA 
GCATCTGAAG CTGACTACCA GTATTACCCA GGCAAAGGTT TGTTGGGAAC CACGTCTCGG 
CTGGACCAGT CCAACTCCAT CTCGTCCTAC GTCACCTTGA GGAGGGCTCC CGGCAGCGCC 
GCACCAAAGG ACCGACCCAA AAGCGCCTTT GAGCGCCTGT CATCCCCCAC GGAGGGCGGC 
CCGCCCCCCA GCAGCCAAAC CCGAGGCCGG ATGACTGCCG AGGAGCAGCT GGAGCGCATG 
AAGCGCCACC AGAGGGCGCT GGTCCGCGAG CGCAAGAGGA ACCTGAGCCA CGGCGAGCGC 
TCTGGCGGCG GGGGCCCGGC AGCATCAGGC TCTCAGCGCT CGTCCTCGGC GTCGTCCCGA 
CTGCCCTCCA GCCCCTGCGT GTTCGACTGG CAGCAGGAGC GACCAGGAGC TGAAGGTCAG 
AGCGACGAAG GCCTGAATCT GGTCAGAGAG AGGGAGACGA GGGACTCTAA TGAGTGGGTG 
ACGGTCACCG CCAGGCGCAT CCAGGAGGCC GACCTGGAGC CCCTCGACTA CGACCTGGAC 
ATCAGCCGGG AGCTGTCCAA ACCGGACAAA GTTGTGATCC CGGAGCGCTA CGTGGAGTCG 
GACCCAGAGG AGCCTCTGAG TCCAGAGGAG CTGGAGGAAC GCGGCCGGCG CGCGGAACGC 
ATCAAGAACC TTCTCACCAG GTCCAGTGTC CAGAACATCC AGCCATCAGC ATCGCTGGAA 
TTCAACGAGC TGGACAACGC CATGGAGGAG CAGGAGAGGA TCATGAGCGT CTCCTACGCG 
CTGGCCTCAG AGGCTTCACG CAAGAGTAAA CTGGTCGCAG CCAAAGCTGC TGCTCAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_008410804.2 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_008409026.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OPo196452
Clone ID Related Accession (Same CDS sequence)	XM_008410804.2
Accession Version	XM_008410804.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 21, 2016 this sequence version replaced XM_008410804.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTCAT ACATTTACAA GCAGAGCTCT GTGATTGGCT CGCAGGCAGA GCACACTGGC 
ATGCGGACGT ATTACTTCAG CGCTGACACC CAGGAGGACA TGAGCACCTG GCTGAAGGCC 
ATGAACCAGG CATCGCTGAT GCAGAACCAC CCCGACAGGC TCAGACCCGT TGACCAAGTC 
ACCTCGTTGT TGCAACAAGC TGTCCCGCAG ACAAACCATG TGAATCACCA CAAGATGAAG 
ACCGTAGACT CTGAAACCAC AAAAGCAATT GTCCACGAAG TTCTGCTGGA GCCCATTCAC 
CGTAACACAG AAGAGCTCTG CAGCTTTCAC CAGGACGCCG GCAACTTCAC CCTGGAGCGC 
CACGCGGGGA CCGCCTCTTT GGAGATGGAC AAACAGAGCC CCCTTCCATC CAGACCGCCA 
GCCGCCTCCA CACTCCCTCT GTCGGACCGC GCATCCGCCT CTGCTCCCGT GTCCAGGGTG 
ACGTCCCGCG CTCCGTCCCG CTCCGCCTCG ACATTGCCCT CCAGCCTTTG CATGAGGAAC 
GGCCTGGTGT CCACGCCCAG CCCCATCCTG GAGCCCAACG GCATCGCGGC GGGGACGTAC 
CAGAGGGCGC CGGAGCCGGC AGCCTCCGAC ACGTTCAAGC ATCTGCAGAG GAGGAACACT 
CTGGAGCAGG TGGAGCAGTG GGTCAAAGTT CAGAAGGCTG AGCAAAGGGG CCCGCCCTCC 
AGAGAAAACA CCCTTCCCCG CCGGACGCCG CCCACGCAGA ACAAGTACAC CTCCATGGAT 
GCCTTTCAAA CTTTGCCAAA AACTCCACGC CACAGCCCCC CATCGGGTCG ACTCGGCGAG 
TACAAGTACG CCCACGACCG CCTGAGCCAC TTCCGCCTCA CCCCAGACCA GGGGGGCACG 
GGGTCCAACA CAGTGCTGCA GCTGTACGAG TGGCAGCAGC GCCAACAGTT CCGTCATGGC 
AGCCCCACGG CGCCAATCTA CACCCCGGCC CCGGATTACC CGTTCGGGCC CCGCCCCCCG 
TCCACCGTGC CGCCTTCCTC CTTGTTGCGC AGGTCGGAGG GGTCTCCTCG CTGCGTGTCG 
GTGCCACCGT CGGCCGCAGA CATCCCGCCA CCGGGGCCTC CGCCAGGCAC CAGCAGGACG 
CTGTCCCCCA ACCGAAGGCC GCACACTCCT GCCGAACGGG TGACTGTAAG GCCCGCTGGG 
GACGGGCCCC AGCTGAACGT CCCCTTCACC GTGTCCCCCC GCAGGAGCAA GTCTCAGATG 
CTGAAGGCTG CTACTATTGA GAGACGCTCG ATGCCTTCAA CTGGCTACAT CACTCACACA 
GTCAGCGCTC CCAGCCTTCA TGGCAAGACG CCGGAGGAGC TCACTCTGCT CCTCATTCAA 
CTGCGCCGCC ACCAGGCCAA GATGGCCGCT GTGCGTCAGC AGACCTTAGC TCAGATCCAG 
CGGCTGAACG CTCCCAGAGA GTCCTGCCAC CAAAACCGCC TCCTCACCGC TGTCGCCGTT 
AGCGACAGCC CCCTCCTCCT CAGCGCCACC GCGTCTCCTG AGTCGCACCT CGAAGATGTC 
GGCCTCTGCA GCGTCCAGGC TGATGACACG TACATGCAGC TGAAGAAGGA CCTGGAATAT 
CTGGACCTAA AGATCAGAGC TCTAGAGCCG CTGATCCTCG CAGTTCACAG TTTACTACTG 
CACTGCACTG CTGGGCCACT CAGGCCTTTA ATGAACGTAG CTGGAAGTCA GGTGCTGAAG 
GAGGCGGGGA AGCCTGTCAA GGTTGCAGAA AGCGATGTGG ATGTGAAGTT GAGTCGGTTA 
TGTGAGCAGG ACAAGATTCT GAAGGACCTG GAGGCGAGGA TCAGCTCCCT GAAGAACGAC 
AAGGACAAGT TGGAGAGAGT GCTTGACGTG TCTCACCAGC AGATGGAGCA GTACCAGGAG 
CAGCCAGCTC ATGTCCACAA GATCGCCTAC CAGCAGAGGC TGCTGCAGGA GGACCTGGTG 
ACCATCAGGG CCCAGATTTC CCGCGTCTCA ACGGAAATGG CGCGTGCGTG GGAGGAGTAC 
AACCGGCTGG AGCAATCAGT GGAGCTCCTA AGGCTGGCAC TGCAGGCACA CATGGCCCAC 
AACGACACGT CTCAGCAGGA AAAGGCCGAA CTGAAGCGGG AGCTGTGGAG GATTGAGGAC 
GTCATGGGTG GTCTTAGTGC CAGCAAAGCC AACTACAAGA TAACTGTGGA CTCTATTCAG 
AATCCAGCAG ACAGGAGATT AGTGCCTTCG TTGTCGGACC AGGCATTGCC TTCCGATGGC 
GCGGAGGTCC AGCCTCCCCC CCGCAGCTCC ATCTCCCACA CTTTACCTCA CAGCACCGTG 
CCAAAGTGGG CAGAGGATGA CGCCCCGCCC AGGCCGCCAC TGCCTCGTCT CTATGACTAC 
GAGGAGATGC CCCCCGTTGT GCCCCCGCTC CCCAAGGAGG CCTCGGTCAT CCGCCACACA 
TCGGTGCGAG GGCTCAAACG CCAGTCGGAC GAGAGGAAGC GGGACAGGGA GGGCGGCCAG 
TACATTGCCA ATGGAGACTG TAAGACTGAT TTGAGGTCAT ACCTGAGCGA ACCAGAGCTG 
CCAGGAAGCA GCCAGCCAAT CACAGCATCT GAAGCTGACT ACCAGTATTA CCCAGGCAAA 
GGTTTGTTGG GAACCACGTC TCGGCTGGAC CAGTCCAACT CCATCTCGTC CTACGTCACC 
TTGAGGAGGG CTCCCGGCAG CGCCGCACCA AAGGACCGAC CCAAAAGCGC CTTTGAGCGC 
CTGTCATCCC CCACGGAGGG CGGCCCGCCC CCCAGCAGCC AAACCCGAGG CCGGATGACT 
GCCGAGGAGC AGCTGGAGCG CATGAAGCGC CACCAGAGGG CGCTGGTCCG CGAGCGCAAG 
AGGAACCTGA GCCACGGCGA GCGCTCTGGC GGCGGGGGCC CGGCAGCATC AGGCTCTCAG 
CGCTCGTCCT CGGCGTCGTC CCGACTGCCC TCCAGCCCCT GCGTGTTCGA CTGGCAGCAG 
GAGCGACCAG GAGCTGAAGG TCAGAGCGAC GAAGGCCTGA ATCTGGTCAG AGAGAGGGAG 
ACGAGGGACT CTAATGAGTG GGTGACGGTC ACCGCCAGGC GCATCCAGGA GGCCGACCTG 
GAGCCCCTCG ACTACGACCT GGACATCAGC CGGGAGCTGT CCAAACCGGA CAAAGTTGTG 
ATCCCGGAGC GCTACGTGGA GTCGGACCCA GAGGAGCCTC TGAGTCCAGA GGAGCTGGAG 
GAACGCGGCC GGCGCGCGGA ACGCATCAAG AACCTTCTCA CCAGGTCCAG TGTCCAGAAC 
ATCCAGCCAT CAGCATCGCT GGAATTCAAC GAGCTGGACA ACGCCATGGA GGAGCAGGAG 
AGGATCATGA GCGTCTCCTA CGCGCTGGCC TCAGAGGCTT CACGCAAGAG TAAACTGGTC 
GCAGCCAAAG CTGCTGCTCA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008409026.1
CDS	2186..5632
Translation	MRSYIYKQSSVIGSQAEHTGMRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_008410804.2
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008410804.2

ORF Insert Sequence:

ATGCGTTCAT ACATTTACAA GCAGAGCTCT GTGATTGGCT CGCAGGCAGA GCACACTGGC 
ATGCGGACGT ATTACTTCAG CGCTGACACC CAGGAGGACA TGAGCACCTG GCTGAAGGCC 
ATGAACCAGG CATCGCTGAT GCAGAACCAC CCCGACAGGC TCAGACCCGT TGACCAAGTC 
ACCTCGTTGT TGCAACAAGC TGTCCCGCAG ACAAACCATG TGAATCACCA CAAGATGAAG 
ACCGTAGACT CTGAAACCAC AAAAGCAATT GTCCACGAAG TTCTGCTGGA GCCCATTCAC 
CGTAACACAG AAGAGCTCTG CAGCTTTCAC CAGGACGCCG GCAACTTCAC CCTGGAGCGC 
CACGCGGGGA CCGCCTCTTT GGAGATGGAC AAACAGAGCC CCCTTCCATC CAGACCGCCA 
GCCGCCTCCA CACTCCCTCT GTCGGACCGC GCATCCGCCT CTGCTCCCGT GTCCAGGGTG 
ACGTCCCGCG CTCCGTCCCG CTCCGCCTCG ACATTGCCCT CCAGCCTTTG CATGAGGAAC 
GGCCTGGTGT CCACGCCCAG CCCCATCCTG GAGCCCAACG GCATCGCGGC GGGGACGTAC 
CAGAGGGCGC CGGAGCCGGC AGCCTCCGAC ACGTTCAAGC ATCTGCAGAG GAGGAACACT 
CTGGAGCAGG TGGAGCAGTG GGTCAAAGTT CAGAAGGCTG AGCAAAGGGG CCCGCCCTCC 
AGAGAAAACA CCCTTCCCCG CCGGACGCCG CCCACGCAGA ACAAGTACAC CTCCATGGAT 
GCCTTTCAAA CTTTGCCAAA AACTCCACGC CACAGCCCCC CATCGGGTCG ACTCGGCGAG 
TACAAGTACG CCCACGACCG CCTGAGCCAC TTCCGCCTCA CCCCAGACCA GGGGGGCACG 
GGGTCCAACA CAGTGCTGCA GCTGTACGAG TGGCAGCAGC GCCAACAGTT CCGTCATGGC 
AGCCCCACGG CGCCAATCTA CACCCCGGCC CCGGATTACC CGTTCGGGCC CCGCCCCCCG 
TCCACCGTGC CGCCTTCCTC CTTGTTGCGC AGGTCGGAGG GGTCTCCTCG CTGCGTGTCG 
GTGCCACCGT CGGCCGCAGA CATCCCGCCA CCGGGGCCTC CGCCAGGCAC CAGCAGGACG 
CTGTCCCCCA ACCGAAGGCC GCACACTCCT GCCGAACGGG TGACTGTAAG GCCCGCTGGG 
GACGGGCCCC AGCTGAACGT CCCCTTCACC GTGTCCCCCC GCAGGAGCAA GTCTCAGATG 
CTGAAGGCTG CTACTATTGA GAGACGCTCG ATGCCTTCAA CTGGCTACAT CACTCACACA 
GTCAGCGCTC CCAGCCTTCA TGGCAAGACG CCGGAGGAGC TCACTCTGCT CCTCATTCAA 
CTGCGCCGCC ACCAGGCCAA GATGGCCGCT GTGCGTCAGC AGACCTTAGC TCAGATCCAG 
CGGCTGAACG CTCCCAGAGA GTCCTGCCAC CAAAACCGCC TCCTCACCGC TGTCGCCGTT 
AGCGACAGCC CCCTCCTCCT CAGCGCCACC GCGTCTCCTG AGTCGCACCT CGAAGATGTC 
GGCCTCTGCA GCGTCCAGGC TGATGACACG TACATGCAGC TGAAGAAGGA CCTGGAATAT 
CTGGACCTAA AGATCAGAGC TCTAGAGCCG CTGATCCTCG CAGTTCACAG TTTACTACTG 
CACTGCACTG CTGGGCCACT CAGGCCTTTA ATGAACGTAG CTGGAAGTCA GGTGCTGAAG 
GAGGCGGGGA AGCCTGTCAA GGTTGCAGAA AGCGATGTGG ATGTGAAGTT GAGTCGGTTA 
TGTGAGCAGG ACAAGATTCT GAAGGACCTG GAGGCGAGGA TCAGCTCCCT GAAGAACGAC 
AAGGACAAGT TGGAGAGAGT GCTTGACGTG TCTCACCAGC AGATGGAGCA GTACCAGGAG 
CAGCCAGCTC ATGTCCACAA GATCGCCTAC CAGCAGAGGC TGCTGCAGGA GGACCTGGTG 
ACCATCAGGG CCCAGATTTC CCGCGTCTCA ACGGAAATGG CGCGTGCGTG GGAGGAGTAC 
AACCGGCTGG AGCAATCAGT GGAGCTCCTA AGGCTGGCAC TGCAGGCACA CATGGCCCAC 
AACGACACGT CTCAGCAGGA AAAGGCCGAA CTGAAGCGGG AGCTGTGGAG GATTGAGGAC 
GTCATGGGTG GTCTTAGTGC CAGCAAAGCC AACTACAAGA TAACTGTGGA CTCTATTCAG 
AATCCAGCAG ACAGGAGATT AGTGCCTTCG TTGTCGGACC AGGCATTGCC TTCCGATGGC 
GCGGAGGTCC AGCCTCCCCC CCGCAGCTCC ATCTCCCACA CTTTACCTCA CAGCACCGTG 
CCAAAGTGGG CAGAGGATGA CGCCCCGCCC AGGCCGCCAC TGCCTCGTCT CTATGACTAC 
GAGGAGATGC CCCCCGTTGT GCCCCCGCTC CCCAAGGAGG CCTCGGTCAT CCGCCACACA 
TCGGTGCGAG GGCTCAAACG CCAGTCGGAC GAGAGGAAGC GGGACAGGGA GGGCGGCCAG 
TACATTGCCA ATGGAGACTG TAAGACTGAT TTGAGGTCAT ACCTGAGCGA ACCAGAGCTG 
CCAGGAAGCA GCCAGCCAAT CACAGCATCT GAAGCTGACT ACCAGTATTA CCCAGGCAAA 
GGTTTGTTGG GAACCACGTC TCGGCTGGAC CAGTCCAACT CCATCTCGTC CTACGTCACC 
TTGAGGAGGG CTCCCGGCAG CGCCGCACCA AAGGACCGAC CCAAAAGCGC CTTTGAGCGC 
CTGTCATCCC CCACGGAGGG CGGCCCGCCC CCCAGCAGCC AAACCCGAGG CCGGATGACT 
GCCGAGGAGC AGCTGGAGCG CATGAAGCGC CACCAGAGGG CGCTGGTCCG CGAGCGCAAG 
AGGAACCTGA GCCACGGCGA GCGCTCTGGC GGCGGGGGCC CGGCAGCATC AGGCTCTCAG 
CGCTCGTCCT CGGCGTCGTC CCGACTGCCC TCCAGCCCCT GCGTGTTCGA CTGGCAGCAG 
GAGCGACCAG GAGCTGAAGG TCAGAGCGAC GAAGGCCTGA ATCTGGTCAG AGAGAGGGAG 
ACGAGGGACT CTAATGAGTG GGTGACGGTC ACCGCCAGGC GCATCCAGGA GGCCGACCTG 
GAGCCCCTCG ACTACGACCT GGACATCAGC CGGGAGCTGT CCAAACCGGA CAAAGTTGTG 
ATCCCGGAGC GCTACGTGGA GTCGGACCCA GAGGAGCCTC TGAGTCCAGA GGAGCTGGAG 
GAACGCGGCC GGCGCGCGGA ACGCATCAAG AACCTTCTCA CCAGGTCCAG TGTCCAGAAC 
ATCCAGCCAT CAGCATCGCT GGAATTCAAC GAGCTGGACA ACGCCATGGA GGAGCAGGAG 
AGGATCATGA GCGTCTCCTA CGCGCTGGCC TCAGAGGCTT CACGCAAGAG TAAACTGGTC 
GCAGCCAAAG CTGCTGCTCA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC103465714 ( XM_017305318.1 ) cDNA ORF clone, Poecilia reticulata(guppy) -> XP_017160807.1 Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X16, mRNA.

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CloneID	OPo196453
Clone ID Related Accession (Same CDS sequence)	XM_017305318.1
Accession Version	XM_017305318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-20
Organism	Poecilia reticulata(guppy)
Product	pleckstrin homology domain-containing family A member 7-like isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_024336.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Poecilia reticulata Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGACGT ATTACTTCAG CGCTGACACC CAGGAGGACA TGAGCACCTG GCTGAAGGCC 
ATGAACCAGG CATCGCTGAT GCAGAACCAC CCCGACAGGC TCAGACCCGT TGACCAAGTC 
ACCTCGTTGT TGCAACAAGC TGTCCCGCAG ACAAACCATG TGAATCACCA CAAGATGAAG 
ACCGTAGACT CTGAAACCAC AAAAGCAATT GTCCACGAAG TTCTGCTGGA GCCCATTCAC 
CGTAACACAG AAGAGCTCTG CAGCTTTCAC CAGGACGCCG GCAACTTCAC CCTGGAGCGC 
CACGCGGGGA CCGCCTCTTT GGAGATGGAC AAACAGAGCC CCCTTCCATC CAGACCGCCA 
GCCGCCTCCA CACTCCCTCT GTCGGACCGC GCATCCGCCT CTGCTCCCGT GTCCAGGGTG 
ACGTCCCGCG CTCCGTCCCG CTCCGCCTCG ACATTGCCCT CCAGCCTTTG CATGAGGAAC 
GGCCTGGTGT CCACGCCCAG CCCCATCCTG GAGCCCAACG GCATCGCGGC GGGGACGTAC 
CAGAGGGCGC CGGAGCCGGC AGCCTCCGAC ACGTTCAAGC ATCTGCAGAG GAGGAACACT 
CTGGAGCAGG TGGAGCAGTG GGTCAAAGTT CAGAAGGCTG AGCAAAGGGG CCCGCCCTCC 
AGAGAAAACA CCCTTCCCCG CCGGACGCCG CCCACGCAGA ACAAGTACAC CTCCATGGAT 
GCCTTTCAAA CTTTGCCAAA AACTCCACGC CACAGCCCCC CATCGGGTCG ACTCGGCGAG 
TACAAGTACG CCCACGACCG CCTGAGCCAC TTCCGCCTCA CCCCAGACCA GGGGGGCACG 
GGGTCCAACA CAGTGCTGCA GCTGTACGAG TGGCAGCAGC GCCAACAGTT CCGTCATGGC 
AGCCCCACGG CGCCAATCTA CACCCCGGCC CCGGATTACC CGTTCGGGCC CCGCCCCCCG 
TCCACCGTGC CGCCTTCCTC CTTGTTGCGC AGGTCGGAGG GGTCTCCTCG CTGCGTGTCG 
GTGCCACCGT CGGCCGCAGA CATCCCGCCA CCGGGGCCTC CGCCAGGCAC CAGCAGGACG 
CTGTCCCCCA ACCGAAGGCC GCACACTCCT GCCGAACGGG TGACTGTAAG GCCCGCTGGG 
GACGGGCCCC AGCTGAACGT CCCCTTCACC GTGTCCCCCC GCAGGAGCAA GTCTCAGATG 
CTGAAGGCTG CTACTATTGA GAGACGCTCG ATGCCTTCAA CTGGCTACAT CACTCACACA 
GTCAGCGCTC CCAGCCTTCA TGGCAAGACG CCGGAGGAGC TCACTCTGCT CCTCATTCAA 
CTGCGCCGCC ACCAGGCCAA GATGGCCGCT GTGCGTCAGC AGACCTTAGC TCAGATCCAG 
CGGCTGAACG CTCCCAGAGA GTCCTGCCAC CAAAACCGCC TCCTCACCGC TGTCGCCGTT 
AGCGACAGCC CCCTCCTCCT CAGCGCCACC GCGTCTCCTG AGTCGCACCT CGAAGATGTC 
GGCCTCTGCA GCGTCCAGGC TGATGACACG TACATGCAGC TGAAGAAGGA CCTGGAATAT 
CTGGACCTAA AGATCAGAGC TCTAGAGCCG CTGATCCTCG CAGTTCACAG TTTACTACTG 
CACTGCACTG CTGGGCCACT CAGGCCTTTA ATGAACGTAG CTGGAAGTCA GGTGCTGAAG 
GAGGCGGGGA AGCCTGTCAA GGTTGCAGAA AGCGATGTGG ATGTGAAGTT GAGTCGGTTA 
TGTGAGCAGG ACAAGATTCT GAAGGACCTG GAGGCGAGGA TCAGCTCCCT GAAGAACGAC 
AAGGACAAGT TGGAGAGAGT GCTTGACGTG TCTCACCAGC AGATGGAGCA GTACCAGGAG 
CAGCCAGCTC ATGTCCACAA GATCGCCTAC CAGCAGAGGC TGCTGCAGGA GGACCTGGTG 
ACCATCAGGG CCCAGATTTC CCGCGTCTCA ACGGAAATGG CGCGTGCGTG GGAGGAGTAC 
AACCGGCTGG AGCAATCAGT GGAGCTCCTA AGGCTGGCAC TGCAGGCACA CATGGCCCAC 
AACGACACGT CTCAGCAGGA AAAGGCCGAA CTGAAGCGGG AGCTGTGGAG GATTGAGGAC 
GTCATGGGTG GTCTTAGTGC CAGCAAAGCC AACTACAAGA TAACTGTGGA CTCTATTCAG 
AATCCAGCAG ACAGGAGATT AGTGCCTTCG TTGTCGGACC AGGCATTGCC TTCCGATGGC 
GCGGAGGTCC AGCCTCCCCC CCGCAGCTCC ATCTCCCACA CTTTACCTCA CAGCACCGTG 
CCAAAGTGGG CAGAGGATGA CGCCCCGCCC AGGCCGCCAC TGCCTCGTCT CTATGACTAC 
GAGGAGATGC CCCCCGTTGT GCCCCCGCTC CCCAAGGAGG CCTCGGTCAT CCGCCACACA 
TCGGTGCGAG GGCTCAAACG CCAGTCGGAC GAGAGGAAGC GGGACAGGGA GGGCGGCCAG 
TACATTGCCA ATGGAGACTG TAAGACTGAT TTGAGGTCAT ACCTGAGCGA ACCAGAGCTG 
CCAGGAAGCA GCCAGCCAAT CACAGCATCT GAAGCTGACT ACCAGTATTA CCCAGGCAAA 
GGTTTGTTGG GAACCACGTC TCGGCTGGAC CAGTCCAACT CCATCTCGTC CTACGTCACC 
TTGAGGAGGG CTCCCGGCAG CGCCGCACCA AAGGACCGAC CCAAAAGCGC CTTTGAGCGC 
CTGTCATCCC CCACGGAGGG CGGCCCGCCC CCCAGCAGCC AAACCCGAGG CCGGATGACT 
GCCGAGGAGC AGCTGGAGCG CATGAAGCGC CACCAGAGGG CGCTGGTCCG CGAGCGCAAG 
AGGAACCTGA GCCACGGCGA GCGCTCTGGC GGCGGGGGCC CGGCAGCATC AGGCTCTCAG 
CGCTCGTCCT CGGCGTCGTC CCGACTGCCC TCCAGCCCCT GCGTGTTCGA CTGGCAGCAG 
GAGCGACCAG GAGCTGAAGG TCAGAGCGAC GAAGGCCTGA ATCTGGTCAG AGAGAGGGAG 
ACGAGGGACT CTAATGAGTG GGTGACGGTC ACCGCCAGGC GCATCCAGGA GGCCGACCTG 
GAGCCCCTCG ACTACGACCT GGACATCAGC CGGGAGCTGT CCAAACCGGA CAAAGTTGTG 
ATCCCGGAGC GCTACGTGGA GTCGGACCCA GAGGAGCCTC TGAGTCCAGA GGAGCTGGAG 
GAACGCGGCC GGCGCGCGGA ACGCATCAAG AACCTTCTCA CCAGGTCCAG TGTCCAGAAC 
ATCCAGCCAT CAGCATCGCT GGAATTCAAC GAGCTGGACA ACGCCATGGA GGAGCAGGAG 
AGGATCATGA GCGTCTCCTA CGCGCTGGCC TCAGAGGCTT CACGCAAGAG TAAACTGGTC 
GCAGCCAAAG CTGCTGCTCA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017160807.1
CDS	2186..5572
Translation	MRTYYFSADTQEDMSTWLKAMNQASLMQNHPDRLRPVDQVTSLLQQAVPQTNHVNHHKMKTVDSETTKAIVHEVLLEPIHRNTEELCSFHQDAGNFTLERHAGTASLEMDKQSPLPSRPPAASTLPLSDRASASAPVSRVTSRAPSRSASTLPSSLCMRNGLVSTPSPILEPNGIAAGTYQRAPEPAASDTFKHLQRRNTLEQVEQWVKVQKAEQRGPPSRENTLPRRTPPTQNKYTSMDAFQTLPKTPRHSPPSGRLGEYKYAHDRLSHFRLTPDQGGTGSNTVLQLYEWQQRQQFRHGSPTAPIYTPAPDYPFGPRPPSTVPPSSLLRRSEGSPRCVSVPPSAADIPPPGPPPGTSRTLSPNRRPHTPAERVTVRPAGDGPQLNVPFTVSPRRSKSQMLKAATIERRSMPSTGYITHTVSAPSLHGKTPEELTLLLIQLRRHQAKMAAVRQQTLAQIQRLNAPRESCHQNRLLTAVAVSDSPLLLSATASPESHLEDVGLCSVQADDTYMQLKKDLEYLDLKIRALEPLILAVHSLLLHCTAGPLRPLMNVAGSQVLKEAGKPVKVAESDVDVKLSRLCEQDKILKDLEARISSLKNDKDKLERVLDVSHQQMEQYQEQPAHVHKIAYQQRLLQEDLVTIRAQISRVSTEMARAWEEYNRLEQSVELLRLALQAHMAHNDTSQQEKAELKRELWRIEDVMGGLSASKANYKITVDSIQNPADRRLVPSLSDQALPSDGAEVQPPPRSSISHTLPHSTVPKWAEDDAPPRPPLPRLYDYEEMPPVVPPLPKEASVIRHTSVRGLKRQSDERKRDREGGQYIANGDCKTDLRSYLSEPELPGSSQPITASEADYQYYPGKGLLGTTSRLDQSNSISSYVTLRRAPGSAAPKDRPKSAFERLSSPTEGGPPPSSQTRGRMTAEEQLERMKRHQRALVRERKRNLSHGERSGGGGPAASGSQRSSSASSRLPSSPCVFDWQQERPGAEGQSDEGLNLVRERETRDSNEWVTVTARRIQEADLEPLDYDLDISRELSKPDKVVIPERYVESDPEEPLSPEELEERGRRAERIKNLLTRSSVQNIQPSASLEFNELDNAMEEQERIMSVSYALASEASRKSKLVAAKAAAQP

Target ORF information:

RefSeq Version	XM_017305318.1
Organism	Poecilia reticulata(guppy)
Definition	Poecilia reticulata pleckstrin homology domain-containing family A member 7-like (LOC103465714), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017305318.1

ORF Insert Sequence:

ATGCGGACGT ATTACTTCAG CGCTGACACC CAGGAGGACA TGAGCACCTG GCTGAAGGCC 
ATGAACCAGG CATCGCTGAT GCAGAACCAC CCCGACAGGC TCAGACCCGT TGACCAAGTC 
ACCTCGTTGT TGCAACAAGC TGTCCCGCAG ACAAACCATG TGAATCACCA CAAGATGAAG 
ACCGTAGACT CTGAAACCAC AAAAGCAATT GTCCACGAAG TTCTGCTGGA GCCCATTCAC 
CGTAACACAG AAGAGCTCTG CAGCTTTCAC CAGGACGCCG GCAACTTCAC CCTGGAGCGC 
CACGCGGGGA CCGCCTCTTT GGAGATGGAC AAACAGAGCC CCCTTCCATC CAGACCGCCA 
GCCGCCTCCA CACTCCCTCT GTCGGACCGC GCATCCGCCT CTGCTCCCGT GTCCAGGGTG 
ACGTCCCGCG CTCCGTCCCG CTCCGCCTCG ACATTGCCCT CCAGCCTTTG CATGAGGAAC 
GGCCTGGTGT CCACGCCCAG CCCCATCCTG GAGCCCAACG GCATCGCGGC GGGGACGTAC 
CAGAGGGCGC CGGAGCCGGC AGCCTCCGAC ACGTTCAAGC ATCTGCAGAG GAGGAACACT 
CTGGAGCAGG TGGAGCAGTG GGTCAAAGTT CAGAAGGCTG AGCAAAGGGG CCCGCCCTCC 
AGAGAAAACA CCCTTCCCCG CCGGACGCCG CCCACGCAGA ACAAGTACAC CTCCATGGAT 
GCCTTTCAAA CTTTGCCAAA AACTCCACGC CACAGCCCCC CATCGGGTCG ACTCGGCGAG 
TACAAGTACG CCCACGACCG CCTGAGCCAC TTCCGCCTCA CCCCAGACCA GGGGGGCACG 
GGGTCCAACA CAGTGCTGCA GCTGTACGAG TGGCAGCAGC GCCAACAGTT CCGTCATGGC 
AGCCCCACGG CGCCAATCTA CACCCCGGCC CCGGATTACC CGTTCGGGCC CCGCCCCCCG 
TCCACCGTGC CGCCTTCCTC CTTGTTGCGC AGGTCGGAGG GGTCTCCTCG CTGCGTGTCG 
GTGCCACCGT CGGCCGCAGA CATCCCGCCA CCGGGGCCTC CGCCAGGCAC CAGCAGGACG 
CTGTCCCCCA ACCGAAGGCC GCACACTCCT GCCGAACGGG TGACTGTAAG GCCCGCTGGG 
GACGGGCCCC AGCTGAACGT CCCCTTCACC GTGTCCCCCC GCAGGAGCAA GTCTCAGATG 
CTGAAGGCTG CTACTATTGA GAGACGCTCG ATGCCTTCAA CTGGCTACAT CACTCACACA 
GTCAGCGCTC CCAGCCTTCA TGGCAAGACG CCGGAGGAGC TCACTCTGCT CCTCATTCAA 
CTGCGCCGCC ACCAGGCCAA GATGGCCGCT GTGCGTCAGC AGACCTTAGC TCAGATCCAG 
CGGCTGAACG CTCCCAGAGA GTCCTGCCAC CAAAACCGCC TCCTCACCGC TGTCGCCGTT 
AGCGACAGCC CCCTCCTCCT CAGCGCCACC GCGTCTCCTG AGTCGCACCT CGAAGATGTC 
GGCCTCTGCA GCGTCCAGGC TGATGACACG TACATGCAGC TGAAGAAGGA CCTGGAATAT 
CTGGACCTAA AGATCAGAGC TCTAGAGCCG CTGATCCTCG CAGTTCACAG TTTACTACTG 
CACTGCACTG CTGGGCCACT CAGGCCTTTA ATGAACGTAG CTGGAAGTCA GGTGCTGAAG 
GAGGCGGGGA AGCCTGTCAA GGTTGCAGAA AGCGATGTGG ATGTGAAGTT GAGTCGGTTA 
TGTGAGCAGG ACAAGATTCT GAAGGACCTG GAGGCGAGGA TCAGCTCCCT GAAGAACGAC 
AAGGACAAGT TGGAGAGAGT GCTTGACGTG TCTCACCAGC AGATGGAGCA GTACCAGGAG 
CAGCCAGCTC ATGTCCACAA GATCGCCTAC CAGCAGAGGC TGCTGCAGGA GGACCTGGTG 
ACCATCAGGG CCCAGATTTC CCGCGTCTCA ACGGAAATGG CGCGTGCGTG GGAGGAGTAC 
AACCGGCTGG AGCAATCAGT GGAGCTCCTA AGGCTGGCAC TGCAGGCACA CATGGCCCAC 
AACGACACGT CTCAGCAGGA AAAGGCCGAA CTGAAGCGGG AGCTGTGGAG GATTGAGGAC 
GTCATGGGTG GTCTTAGTGC CAGCAAAGCC AACTACAAGA TAACTGTGGA CTCTATTCAG 
AATCCAGCAG ACAGGAGATT AGTGCCTTCG TTGTCGGACC AGGCATTGCC TTCCGATGGC 
GCGGAGGTCC AGCCTCCCCC CCGCAGCTCC ATCTCCCACA CTTTACCTCA CAGCACCGTG 
CCAAAGTGGG CAGAGGATGA CGCCCCGCCC AGGCCGCCAC TGCCTCGTCT CTATGACTAC 
GAGGAGATGC CCCCCGTTGT GCCCCCGCTC CCCAAGGAGG CCTCGGTCAT CCGCCACACA 
TCGGTGCGAG GGCTCAAACG CCAGTCGGAC GAGAGGAAGC GGGACAGGGA GGGCGGCCAG 
TACATTGCCA ATGGAGACTG TAAGACTGAT TTGAGGTCAT ACCTGAGCGA ACCAGAGCTG 
CCAGGAAGCA GCCAGCCAAT CACAGCATCT GAAGCTGACT ACCAGTATTA CCCAGGCAAA 
GGTTTGTTGG GAACCACGTC TCGGCTGGAC CAGTCCAACT CCATCTCGTC CTACGTCACC 
TTGAGGAGGG CTCCCGGCAG CGCCGCACCA AAGGACCGAC CCAAAAGCGC CTTTGAGCGC 
CTGTCATCCC CCACGGAGGG CGGCCCGCCC CCCAGCAGCC AAACCCGAGG CCGGATGACT 
GCCGAGGAGC AGCTGGAGCG CATGAAGCGC CACCAGAGGG CGCTGGTCCG CGAGCGCAAG 
AGGAACCTGA GCCACGGCGA GCGCTCTGGC GGCGGGGGCC CGGCAGCATC AGGCTCTCAG 
CGCTCGTCCT CGGCGTCGTC CCGACTGCCC TCCAGCCCCT GCGTGTTCGA CTGGCAGCAG 
GAGCGACCAG GAGCTGAAGG TCAGAGCGAC GAAGGCCTGA ATCTGGTCAG AGAGAGGGAG 
ACGAGGGACT CTAATGAGTG GGTGACGGTC ACCGCCAGGC GCATCCAGGA GGCCGACCTG 
GAGCCCCTCG ACTACGACCT GGACATCAGC CGGGAGCTGT CCAAACCGGA CAAAGTTGTG 
ATCCCGGAGC GCTACGTGGA GTCGGACCCA GAGGAGCCTC TGAGTCCAGA GGAGCTGGAG 
GAACGCGGCC GGCGCGCGGA ACGCATCAAG AACCTTCTCA CCAGGTCCAG TGTCCAGAAC 
ATCCAGCCAT CAGCATCGCT GGAATTCAAC GAGCTGGACA ACGCCATGGA GGAGCAGGAG 
AGGATCATGA GCGTCTCCTA CGCGCTGGCC TCAGAGGCTT CACGCAAGAG TAAACTGGTC 
GCAGCCAAAG CTGCTGCTCA GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC103465714 cDNA ORF clone, Poecilia reticulata(guppy)

Gene

Clones

gRNAs

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