Prss32 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prss32
Entrez Gene ID	69814
Full Name	protease, serine 32
Synonyms	2010001P08Rik,mT5,tryptase-5
General protein information	Preferred Names protease, serine 32 Names protease, serine, 32 eosinophil serine protease testisin tryptase 5
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 12.09 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_027220.2	NP_081496.2	protease, serine, 32 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Prss32	NP_081496.2
Canis lupus familiaris (dog)	OVCH1	XP_005622141.1
Rattus norvegicus (Norway rat)	Prss32	NP_001100453.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of a subgroup of glycosylphosphatidylinositol-anchored tryptases.
Wong GW, Stevens RL
Biochemical and biophysical research communications336(2)579-84(2005 Oct)

Mouse chromosome 17A3.3 contains 13 genes that encode functional tryptic-like serine proteases with distinct tissue and cell expression patterns.
Wong GW, Yasuda S, Morokawa N, Li L, Stevens RL
The Journal of biological chemistry279(4)2438-52(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Prss32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prss32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu03891	NM_027220.2 Latest version!	Mus musculus protease, serine 32 (Prss32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prss32 ( NM_027220.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_081496.2 Mus musculus protease, serine 32 (Prss32), mRNA.

Price & Availability↑

CloneID	OMu03891
Clone ID Related Accession (Same CDS sequence)	NM_027220.2
Accession Version	NM_027220.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Mus musculus(house mouse)
Product	protease, serine, 32 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC122821.4. On Nov 15, 2007 this sequence version replaced NM_027220.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC024903.1, BQ922964.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849376, SAMN00849377 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG CTCTGCCTGC CATCCTATTT ACCTTCTTTC CTGGAGTTCT TCTGGGGAGT 
GAGGTCCTCC CAACCGACTC TGACAGCCCG TCTACCACGA CAGGCAGGAG GAGCATTGAC 
TTGGACTCAG TGTGTGGACG TCCCAGGACA TCAGGCCGCA TCGTGTCAGG ACAAGATGCC 
CAGCTGGGCC GGTGGCCTTG GCAGGTCAGC GTGCGGGAGA ATGGGGCGCA CGTGTGTGGG 
GGATCTCTGA TTGCTGAGGA CTGGGTGTTG ACTGCTGCCC ATTGCTTCAA CCAGGGCCAG 
TCCCTGTCTA TCTACACAGT ACTGCTGGGT ACCATCTCCT CCTACCCTGA GGACAATGAG 
CCGAAGGAGC TGAGAGCTGT GGCCCAGTTT ATCAAGCACC CAAGCTATTC AGCGGACGAG 
CACAGCAGTG GAGACATTGC CCTGGTGCAG CTGGCTTCAC CCATCTCCTT CAATGACTAC 
ATGCTTCCAG TCTGCCTCCC GAAACCTGGC GACCCCCTGG ATCCTGGCAC CATGTGCTGG 
GTCACTGGCT GGGGACACAT TGGCACAAAT CAACCGCTCC CACCACCCTT TACCCTGCAG 
GAGTTGCAGG TGCCTCTCAT TGATGCTGAG ACCTGCAATA CCTACTACCA GGAGAACTCC 
ATTCCCGGCA CGGAGCCAGT CATCCTTGAA GGCATGCTGT GTGCTGGTTT CCAGGAAGGC 
AAGAAGGATG CTTGTAATGG TGATTCCGGA GGTCCCCTAG TCTGTGACAT TAATGATGTC 
TGGATCCAGG CAGGGGTGGT GAGTTGGGGA TCTGATTGTG CTCTGTTCAA GAGGCCAGGT 
GTTTACACCA ACGTCAGTGT CTACATCTCA TGGATTCAGA ACACAATGTG GAATTTACCC 
ATGGAAGGAA GAGGTTTTTC CCCCAGCCTT TCAGGGACTC CACTCTTGGG CCTGTTGCTG 
TTTCTCACAT CCCTCAGTAG CGCCTTCTTC CTCCTGGGAC CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_081496.2
CDS	26..1030
Translation	MELALPAILFTFFPGVLLGSEVLPTDSDSPSTTTGRRSIDLDSVCGRPRTSGRIVSGQDAQLGRWPWQVSVRENGAHVCGGSLIAEDWVLTAAHCFNQGQSLSIYTVLLGTISSYPEDNEPKELRAVAQFIKHPSYSADEHSSGDIALVQLASPISFNDYMLPVCLPKPGDPLDPGTMCWVTGWGHIGTNQPLPPPFTLQELQVPLIDAETCNTYYQENSIPGTEPVILEGMLCAGFQEGKKDACNGDSGGPLVCDINDVWIQAGVVSWGSDCALFKRPGVYTNVSVYISWIQNTMWNLPMEGRGFSPSLSGTPLLGLLLFLTSLSSAFFLLGP

Target ORF information:

RefSeq Version	NM_027220.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus protease, serine 32 (Prss32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_027220.2

ORF Insert Sequence:

ATGGAGCTGG CTCTGCCTGC CATCCTATTT ACCTTCTTTC CTGGAGTTCT TCTGGGGAGT 
GAGGTCCTCC CAACCGACTC TGACAGCCCG TCTACCACGA CAGGCAGGAG GAGCATTGAC 
TTGGACTCAG TGTGTGGACG TCCCAGGACA TCAGGCCGCA TCGTGTCAGG ACAAGATGCC 
CAGCTGGGCC GGTGGCCTTG GCAGGTCAGC GTGCGGGAGA ATGGGGCGCA CGTGTGTGGG 
GGATCTCTGA TTGCTGAGGA CTGGGTGTTG ACTGCTGCCC ATTGCTTCAA CCAGGGCCAG 
TCCCTGTCTA TCTACACAGT ACTGCTGGGT ACCATCTCCT CCTACCCTGA GGACAATGAG 
CCGAAGGAGC TGAGAGCTGT GGCCCAGTTT ATCAAGCACC CAAGCTATTC AGCGGACGAG 
CACAGCAGTG GAGACATTGC CCTGGTGCAG CTGGCTTCAC CCATCTCCTT CAATGACTAC 
ATGCTTCCAG TCTGCCTCCC GAAACCTGGC GACCCCCTGG ATCCTGGCAC CATGTGCTGG 
GTCACTGGCT GGGGACACAT TGGCACAAAT CAACCGCTCC CACCACCCTT TACCCTGCAG 
GAGTTGCAGG TGCCTCTCAT TGATGCTGAG ACCTGCAATA CCTACTACCA GGAGAACTCC 
ATTCCCGGCA CGGAGCCAGT CATCCTTGAA GGCATGCTGT GTGCTGGTTT CCAGGAAGGC 
AAGAAGGATG CTTGTAATGG TGATTCCGGA GGTCCCCTAG TCTGTGACAT TAATGATGTC 
TGGATCCAGG CAGGGGTGGT GAGTTGGGGA TCTGATTGTG CTCTGTTCAA GAGGCCAGGT 
GTTTACACCA ACGTCAGTGT CTACATCTCA TGGATTCAGA ACACAATGTG GAATTTACCC 
ATGGAAGGAA GAGGTTTTTC CCCCAGCCTT TCAGGGACTC CACTCTTGGG CCTGTTGCTG 
TTTCTCACAT CCCTCAGTAG CGCCTTCTTC CTCCTGGGAC CCTAA