TMEM27 cDNA ORF clone, Mus musculus(House mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	TMEM27
Entrez Gene ID	57394
Full Name	transmembrane protein 27
Synonyms	0610008J07Rik,NX-17,NX17
General protein information	Preferred Names transmembrane protein 27 Names collectrin kidney-specific membrane protein
Gene Type	protein-coding
Organism	Mus musculus(House mouse)
Genome	Chromosome: X Map Location: X\|X F5

mRNA and Protein(s)

mRNA	Protein	Name
NM_020626.2	NP_065651.1	collectrin isoform 1 precursor
NM_001313719.1	NP_001300648.1	collectrin isoform 2
XM_006528923.1	XP_006528986.1	collectrin isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TMEM27	XP_854205.1
Bos taurus (cattle)	TMEM27	NP_001069071.1
Rattus norvegicus (Norway rat)	Tmem27	NP_066125.1
Xenopus tropicalis (tropical clawed frog)	tmem27	XP_002938294.2
Homo sapiens (human)	TMEM27	NP_065716.1
	TMEM27	XP_001101718.1
Gallus gallus (chicken)	TMEM27	XP_416821.4
Danio rerio (zebrafish)	si:dkey-194e6.1	NP_001132930.1
Mus musculus (house mouse)	Tmem27	NP_065651.1

Gene Ontology
Bibliography

Related articles in PubMed

Cross-Presentation of Soluble and Cell-Associated Antigen by Murine Hepatocytes Is Enhanced by Collectrin Expression.
Dolina JS, Cechova S, Rudy CK, Sung SJ, Tang WW, Lee J, Hahn YS, Le TH
Journal of immunology (Baltimore, Md. : 1950)198(6)2341-2351(2017 Mar)

?-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development.
Abdellatif AM, Oishi H, Itagaki T, Jung Y, Shawki HH, Okita Y, Hasegawa Y, Suzuki H, El-Morsy SE, El-Sayed MA, Shoaib MB, Sugiyama F, Takahashi S
PloS one11(2)e0150010(2016)

Molecular basis for the interaction of the mammalian amino acid transporters B0AT1 and B0AT3 with their ancillary protein collectrin.
Fairweather SJ, Br?er A, Subramanian N, Tumer E, Cheng Q, Schmoll D, O'Mara ML, Br?er S
The Journal of biological chemistry290(40)24308-25(2015 Oct)

Loss of collectrin, an angiotensin-converting enzyme 2 homolog, uncouples endothelial nitric oxide synthase and causes hypertension and vascular dysfunction.
Cechova S, Zeng Q, Billaud M, Mutchler S, Rudy CK, Straub AC, Chi L, Chan FR, Hu J, Griffiths R, Howell NL, Madsen K, Jensen BL, Palmer LA, Carey RM, Sung SS, Malakauskas SM, Isakson BE, Le TH
Circulation128(16)1770-80(2013 Oct)

ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation.
Hashimoto T, Perlot T, Rehman A, Trichereau J, Ishiguro H, Paolino M, Sigl V, Hanada T, Hanada R, Lipinski S, Wild B, Camargo SM, Singer D, Richter A, Kuba K, Fukamizu A, Schreiber S, Clevers H, Verrey F, Rosenstiel P, Penninger JM
Nature487(7408)477-81(2012 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that Tmem27-mediated cross-presentation supports intrahepatic adaptive antiviral immune responses and may lead to insights into the nature of how the liver acts as a primary site of CD8+ T cell activation
Title: Cross-Presentation of Soluble and Cell-Associated Antigen by Murine Hepatocytes Is Enhanced by Collectrin Expression.

analysis of the interaction of amino acid transporters B0AT1 and B0AT3 with their ancillary protein collectrin
Title: Molecular basis for the interaction of the mammalian amino acid transporters B0AT1 and B0AT3 with their ancillary protein collectrin.

Collectrin is a consequential link between the transport of l-arginine and endothelial nitric oxide synthase uncoupling in hypertension.
Title: Loss of collectrin, an angiotensin-converting enzyme 2 homolog, uncouples endothelial nitric oxide synthase and causes hypertension and vascular dysfunction.

Collectrin and ACE2 in renal and intestinal amino acid transport.
Title: Collectrin and ACE2 in renal and intestinal amino acid transport.

These data indicate the involvement of mitochondrial TCA cycle intermediates, distal to pyruvate, in the regulation of collectrin protein expression in beta-cells.
Title: Glucose enhances collectrin protein expression in insulin-producing MIN6 beta cells.

The following TMEM27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu69728	NM_001313719.1 Latest version!	Mus musculus collectrin, amino acid transport regulator (Cltrn), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu12092	NM_020626.2 Latest version!	Mus musculus collectrin, amino acid transport regulator (Cltrn), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu12092	XM_006528923.1 Latest version!	Mus musculus transmembrane protein 27 (Tmem27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TMEM27 ( NM_001313719.1 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_001300648.1 Mus musculus collectrin, amino acid transport regulator (Cltrn), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu69728
Clone ID Related Accession (Same CDS sequence)	NM_001313719.1
Accession Version	NM_001313719.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Mus musculus(house mouse)
Product	collectrin isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732294.18 and AK148534.1. Transcript Variant: This variant (2) contains an alternate exon in place of the first two exons and lacks an alternate internal in-frame exon compared to variant 1. The resulting isoform (2) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY042134.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849375, SAMN00849385 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGCAT TCTCCATGAG AAAAGTTCCC AACAGAGAAG CAACAGAAAT TTCCCATGTC 
CTGCTTTGCA ACATAACCCA GAGGGTGTCG TTCTGGTTTG TGGTCACGGA CCCTTCCAAC 
AATTATACTC TTCCTGCAGC TGAAGTACAG TCGGCCATAA GGAACAACAA AGGACCACCT 
GGAGTGGAAG ATGCAGAAGA CAAGTGTGAA AACATCATCA CAATAGAAAA TGGCATCCCT 
TGTGATCCCT TGGACATGAA GGGAGGGCAC ATTAATGATG GCTTCTTGAC AGAGGATGAG 
CGCCTCACCC CTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300648.1
CDS	195..512
Translation	MVAFSMRKVPNREATEISHVLLCNITQRVSFWFVVTDPSNNYTLPAAEVQSAIRNNKGPPGVEDAEDKCENIITIENGIPCDPLDMKGGHINDGFLTEDERLTPL

Target ORF information:

RefSeq Version	NM_001313719.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus collectrin, amino acid transport regulator (Cltrn), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313719.1

ORF Insert Sequence:

ATGGTGGCAT TCTCCATGAG AAAAGTTCCC AACAGAGAAG CAACAGAAAT TTCCCATGTC 
CTGCTTTGCA ACATAACCCA GAGGGTGTCG TTCTGGTTTG TGGTCACGGA CCCTTCCAAC 
AATTATACTC TTCCTGCAGC TGAAGTACAG TCGGCCATAA GGAACAACAA AGGACCACCT 
GGAGTGGAAG ATGCAGAAGA CAAGTGTGAA AACATCATCA CAATAGAAAA TGGCATCCCT 
TGTGATCCCT TGGACATGAA GGGAGGGCAC ATTAATGATG GCTTCTTGAC AGAGGATGAG 
CGCCTCACCC CTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM27 ( NM_020626.2 ) cDNA ORF clone, Mus musculus(House mouse) -> NP_065651.1 Mus musculus collectrin, amino acid transport regulator (Cltrn), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu12092
Clone ID Related Accession (Same CDS sequence)	NM_020626.2 , XM_006528923.1
Accession Version	NM_020626.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Mus musculus(house mouse)
Product	collectrin isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732294.18. On Mar 21, 2007 this sequence version replaced NM_020626.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF178085.1, AK148534.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGTGGG CGCTCTTTTT CCTGGTGACT ACTATTCACG CTGAACTCTG CCATCCAGAT 
GCAGAAAATG CCTTTAAAGT AAGACTTAGC ATCAGAGCAG CTCTTGGAGA TAAAGCATAT 
GTCTGGGACA CGGATCAAGA ATACCTCTTC AGAGCAATGG TGGCATTCTC CATGAGAAAA 
GTTCCCAACA GAGAAGCAAC AGAAATTTCC CATGTCCTGC TTTGCAACAT AACCCAGAGG 
GTGTCGTTCT GGTTTGTGGT CACGGACCCT TCCAACAATT ATACTCTTCC TGCAGCTGAA 
GTACAGTCGG CCATAAGAAA GAATAGGAAC CGGATCAACA GTGCATTTTT TCTGGATGAC 
CATACTCTGG AATTTTTAAA AATTCCTTCT ACTCTTGCTC CCCCGATGGA GCCATCTGTG 
CCCGTCTGGA TTATTGTATT CGGTGTGATA TTTTGCATTG TTACAGTTGC AATTGCACTA 
CTGGTTCTAT CTGGAATCCG GCAACGCAGA AGGAACAACA AAGGACCACC TGGAGTGGAA 
GATGCAGAAG ACAAGTGTGA AAACATCATC ACAATAGAAA ATGGCATCCC TTGTGATCCC 
TTGGACATGA AGGGAGGGCA CATTAATGAT GGCTTCTTGA CAGAGGATGA GCGCCTCACC 
CCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065651.1
CDS	87..755
Translation	MLWALFFLVTTIHAELCHPDAENAFKVRLSIRAALGDKAYVWDTDQEYLFRAMVAFSMRKVPNREATEISHVLLCNITQRVSFWFVVTDPSNNYTLPAAEVQSAIRKNRNRINSAFFLDDHTLEFLKIPSTLAPPMEPSVPVWIIVFGVIFCIVTVAIALLVLSGIRQRRRNNKGPPGVEDAEDKCENIITIENGIPCDPLDMKGGHINDGFLTEDERLTPL

Target ORF information:

RefSeq Version	NM_020626.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus collectrin, amino acid transport regulator (Cltrn), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020626.2

ORF Insert Sequence:

ATGTTGTGGG CGCTCTTTTT CCTGGTGACT ACTATTCACG CTGAACTCTG CCATCCAGAT 
GCAGAAAATG CCTTTAAAGT AAGACTTAGC ATCAGAGCAG CTCTTGGAGA TAAAGCATAT 
GTCTGGGACA CGGATCAAGA ATACCTCTTC AGAGCAATGG TGGCATTCTC CATGAGAAAA 
GTTCCCAACA GAGAAGCAAC AGAAATTTCC CATGTCCTGC TTTGCAACAT AACCCAGAGG 
GTGTCGTTCT GGTTTGTGGT CACGGACCCT TCCAACAATT ATACTCTTCC TGCAGCTGAA 
GTACAGTCGG CCATAAGAAA GAATAGGAAC CGGATCAACA GTGCATTTTT TCTGGATGAC 
CATACTCTGG AATTTTTAAA AATTCCTTCT ACTCTTGCTC CCCCGATGGA GCCATCTGTG 
CCCGTCTGGA TTATTGTATT CGGTGTGATA TTTTGCATTG TTACAGTTGC AATTGCACTA 
CTGGTTCTAT CTGGAATCCG GCAACGCAGA AGGAACAACA AAGGACCACC TGGAGTGGAA 
GATGCAGAAG ACAAGTGTGA AAACATCATC ACAATAGAAA ATGGCATCCC TTGTGATCCC 
TTGGACATGA AGGGAGGGCA CATTAATGAT GGCTTCTTGA CAGAGGATGA GCGCCTCACC 
CCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TMEM27 ( XM_006528923.1 ) cDNA ORF clone, Mus musculus(House mouse) -> XP_006528986.1 Mus musculus transmembrane protein 27 (Tmem27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu12092
Clone ID Related Accession (Same CDS sequence)	NM_020626.2 , XM_006528923.1
Accession Version	XM_006528923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-08
Organism	Mus musculus(House mouse)
Product	collectrin isoform X1
Comment

ATGTTGTGGG CGCTCTTTTT CCTGGTGACT ACTATTCACG CTGAACTCTG CCATCCAGAT 
GCAGAAAATG CCTTTAAAGT AAGACTTAGC ATCAGAGCAG CTCTTGGAGA TAAAGCATAT 
GTCTGGGACA CGGATCAAGA ATACCTCTTC AGAGCAATGG TGGCATTCTC CATGAGAAAA 
GTTCCCAACA GAGAAGCAAC AGAAATTTCC CATGTCCTGC TTTGCAACAT AACCCAGAGG 
GTGTCGTTCT GGTTTGTGGT CACGGACCCT TCCAACAATT ATACTCTTCC TGCAGCTGAA 
GTACAGTCGG CCATAAGAAA GAATAGGAAC CGGATCAACA GTGCATTTTT TCTGGATGAC 
CATACTCTGG AATTTTTAAA AATTCCTTCT ACTCTTGCTC CCCCGATGGA GCCATCTGTG 
CCCGTCTGGA TTATTGTATT CGGTGTGATA TTTTGCATTG TTACAGTTGC AATTGCACTA 
CTGGTTCTAT CTGGAATCCG GCAACGCAGA AGGAACAACA AAGGACCACC TGGAGTGGAA 
GATGCAGAAG ACAAGTGTGA AAACATCATC ACAATAGAAA ATGGCATCCC TTGTGATCCC 
TTGGACATGA AGGGAGGGCA CATTAATGAT GGCTTCTTGA CAGAGGATGA GCGCCTCACC 
CCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006528986.1
Translation	MLWALFFLVTTIHAELCHPDAENAFKVRLSIRAALGDKAYVWDTDQEYLFRAMVAFSMRKVPNREATEISHVLLCNITQRVSFWFVVTDPSNNYTLPAAEVQSAIRKNRNRINSAFFLDDHTLEFLKIPSTLAPPMEPSVPVWIIVFGVIFCIVTVAIALLVLSGIRQRRRNNKGPPGVEDAEDKCENIITIENGIPCDPLDMKGGHINDGFLTEDERLTPL

Target ORF information:

RefSeq Version	XM_006528923.1
Organism	Mus musculus(House mouse)
Definition	Mus musculus transmembrane protein 27 (Tmem27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006528923.1

ORF Insert Sequence:

ATGTTGTGGG CGCTCTTTTT CCTGGTGACT ACTATTCACG CTGAACTCTG CCATCCAGAT 
GCAGAAAATG CCTTTAAAGT AAGACTTAGC ATCAGAGCAG CTCTTGGAGA TAAAGCATAT 
GTCTGGGACA CGGATCAAGA ATACCTCTTC AGAGCAATGG TGGCATTCTC CATGAGAAAA 
GTTCCCAACA GAGAAGCAAC AGAAATTTCC CATGTCCTGC TTTGCAACAT AACCCAGAGG 
GTGTCGTTCT GGTTTGTGGT CACGGACCCT TCCAACAATT ATACTCTTCC TGCAGCTGAA 
GTACAGTCGG CCATAAGAAA GAATAGGAAC CGGATCAACA GTGCATTTTT TCTGGATGAC 
CATACTCTGG AATTTTTAAA AATTCCTTCT ACTCTTGCTC CCCCGATGGA GCCATCTGTG 
CCCGTCTGGA TTATTGTATT CGGTGTGATA TTTTGCATTG TTACAGTTGC AATTGCACTA 
CTGGTTCTAT CTGGAATCCG GCAACGCAGA AGGAACAACA AAGGACCACC TGGAGTGGAA 
GATGCAGAAG ACAAGTGTGA AAACATCATC ACAATAGAAA ATGGCATCCC TTGTGATCCC 
TTGGACATGA AGGGAGGGCA CATTAATGAT GGCTTCTTGA CAGAGGATGA GCGCCTCACC 
CCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cross-Presentation of Soluble and Cell-Associated Antigen by Murine Hepatocytes Is Enhanced by Collectrin Expression.
Journal of immunology (Baltimore, Md. : 1950)198(6)2341-2351(2017 Mar)
Dolina JS,Cechova S,Rudy CK,Sung SJ,Tang WW,Lee J,Hahn YS,Le TH

?-Cell-Specific Mafk Overexpression Impairs Pancreatic Endocrine Cell Development.
PloS one11(2)e0150010(2016)
Abdellatif AM,Oishi H,Itagaki T,Jung Y,Shawki HH,Okita Y,Hasegawa Y,Suzuki H,El-Morsy SE,El-Sayed MA,Shoaib MB,Sugiyama F,Takahashi S

Molecular basis for the interaction of the mammalian amino acid transporters B0AT1 and B0AT3 with their ancillary protein collectrin.
The Journal of biological chemistry290(40)24308-25(2015 Oct)
Fairweather SJ,Br?er A,Subramanian N,Tumer E,Cheng Q,Schmoll D,O'Mara ML,Br?er S

Loss of collectrin, an angiotensin-converting enzyme 2 homolog, uncouples endothelial nitric oxide synthase and causes hypertension and vascular dysfunction.
Circulation128(16)1770-80(2013 Oct)
Cechova S,Zeng Q,Billaud M,Mutchler S,Rudy CK,Straub AC,Chi L,Chan FR,Hu J,Griffiths R,Howell NL,Madsen K,Jensen BL,Palmer LA,Carey RM,Sung SS,Malakauskas SM,Isakson BE,Le TH

ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation.
Nature487(7408)477-81(2012 Jul)
Hashimoto T,Perlot T,Rehman A,Trichereau J,Ishiguro H,Paolino M,Sigl V,Hanada T,Hanada R,Lipinski S,Wild B,Camargo SM,Singer D,Richter A,Kuba K,Fukamizu A,Schreiber S,Clevers H,Verrey F,Rosenstiel P,Penninger JM

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TMEM27 cDNA ORF clone, Mus musculus(House mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

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電話

FAX

オンラインフォーム