Cltrn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cltrn
Entrez Gene ID	57395
Full Name	transmembrane protein 27
Synonyms	NX-17,Nx17,collectrin
General protein information	Preferred Names transmembrane protein 27 Names collectrin kidney-specific membrane protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq14
Summary	collecting duct-specific transmembrane glycoprotein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_020976.1	NP_066125.1	collectrin precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TMEM27	XP_854205.1
Bos taurus (cattle)	TMEM27	NP_001069071.1
Rattus norvegicus (Norway rat)	Tmem27	NP_066125.1
Xenopus tropicalis (tropical clawed frog)	tmem27	XP_002938294.2
Homo sapiens (human)	TMEM27	NP_065716.1
	TMEM27	XP_001101718.1
Gallus gallus (chicken)	TMEM27	XP_416821.4
Danio rerio (zebrafish)	si:dkey-194e6.1	NP_001132930.1
Mus musculus (house mouse)	Tmem27	NP_065651.1

Gene Ontology
Bibliography

Related articles in PubMed

Tmem27 dimerization, deglycosylation, plasma membrane depletion, and the extracellular Phe-Phe motif are negative regulators of cleavage by Bace2.
Esterh?zy D, Akpinar P, Stoffel M
Biological chemistry393(6)473-84(2012 May)

Age-related changes in the retinal pigment epithelium (RPE).
Gu X, Neric NJ, Crabb JS, Crabb JW, Bhattacharya SK, Rayborn ME, Hollyfield JG, Bonilha VL
PloS one7(6)e38673(2012)

Bace2 is a ? cell-enriched protease that regulates pancreatic ? cell function and mass.
Esterh?zy D, St?tzer I, Wang H, Rechsteiner MP, Beauchamp J, D?beli H, Hilpert H, Matile H, Prummer M, Schmidt A, Lieske N, Boehm B, Marselli L, Bosco D, Kerr-Conte J, Aebersold R, Spinas GA, Moch H, Migliorini C, Stoffel M
Cell metabolism14(3)365-77(2011 Sep)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

Collectrin is involved in the development of salt-sensitive hypertension by facilitating the membrane trafficking of apical membrane proteins via interaction with soluble N-ethylmaleiamide-sensitive factor attachment protein receptor complex.
Yasuhara A, Wada J, Malakauskas SM, Zhang Y, Eguchi J, Nakatsuka A, Murakami K, Kanzaki M, Teshigawara S, Yamagata K, Le TH, Makino H
Circulation118(21)2146-55(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collectrin expression in the retinal pigment epithelium was significantly modulated during aging.
Title: Age-related changes in the retinal pigment epithelium (RPE).

Upregulation of collectrin by high NaCl independent of aldosterone functionally links to the trafficking of apical membrane proteins via the SNARE complex, and collectrin may be responsible for the sodium retention in salt-sensitive hypertension.
Title: Collectrin is involved in the development of salt-sensitive hypertension by facilitating the membrane trafficking of apical membrane proteins via interaction with soluble N-ethylmaleiamide-sensitive factor attachment protein receptor complex.

The following Cltrn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cltrn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13029	NM_020976.1 Latest version!	Rattus norvegicus collectrin, amino acid transport regulator (Cltrn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cltrn ( NM_020976.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_066125.1 Rattus norvegicus collectrin, amino acid transport regulator (Cltrn), mRNA.

Price & Availability↑

CloneID	ORa13029
Clone ID Related Accession (Same CDS sequence)	NM_020976.1
Accession Version	NM_020976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	collectrin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF178086.1. ##Evidence-Data-START## Transcript exon combination :: AF178086.1, BC078838.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642782 [ECO:0000348] ##Evidence-Data-END##

ATGCTGTGGG CACTCTTTTT CCTGGTGACT ACTATTCACG CTGAACTCTG CCGTCCAGAT 
GCAGAAAATG CCTTTAAAGT AAGACTTAGC ATCAAAGCAG CTCTTGGAGA TAAAGCGTAT 
GTCTGGGACA CAGATGAAGA ATATCTCTTC AGAGCAATGG TGGCATTCTC CATGAGAAAA 
GTTCCCAACA GAGAAGGAAC AGAAATTTCC CACGTCCTGC TTTGCAATGT AACCCAGAGA 
GTGTCATTTT GGTTTGTGGT CACAGACCCT TTGAAAAACC ATACTTTTCC TGCAGTTGAA 
GTACAGTCAG CCATAAGAAT GAACAGGAAA CGGATCAACA GTGCATTCTT TTTGGATGAT 
CATACTCTGG AATTTTTAAA AATTCCTTCC ACTCTTGCTC CCCCGATGGA TCCATCTGTG 
CCCGTCTGGA TTATTGTATT TGGTGTGATA TTTTGCATTG TTACAGTTGC AATTGCACTA 
CTGGTTTTAT CCGGAATCCG GCAACGAAGA AGGAACAAGA AAGGACCACC TGGAGTGGAG 
GATGCAGAAG ACAAGTGTGA AAACATCATC ACAATTGAAA ATGGCATCCC TTGTGATCCC 
TTGGACATGA AGAGAGGGCA CATTAATGAT GGCTTCTTGA CAGAGGATGA GCGTCTCACC 
CCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066125.1
CDS	6..674
Translation	MLWALFFLVTTIHAELCRPDAENAFKVRLSIKAALGDKAYVWDTDEEYLFRAMVAFSMRKVPNREGTEISHVLLCNVTQRVSFWFVVTDPLKNHTFPAVEVQSAIRMNRKRINSAFFLDDHTLEFLKIPSTLAPPMDPSVPVWIIVFGVIFCIVTVAIALLVLSGIRQRRRNKKGPPGVEDAEDKCENIITIENGIPCDPLDMKRGHINDGFLTEDERLTPL

Target ORF information:

RefSeq Version	NM_020976.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collectrin, amino acid transport regulator (Cltrn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020976.1

ORF Insert Sequence:

ATGCTGTGGG CACTCTTTTT CCTGGTGACT ACTATTCACG CTGAACTCTG CCGTCCAGAT 
GCAGAAAATG CCTTTAAAGT AAGACTTAGC ATCAAAGCAG CTCTTGGAGA TAAAGCGTAT 
GTCTGGGACA CAGATGAAGA ATATCTCTTC AGAGCAATGG TGGCATTCTC CATGAGAAAA 
GTTCCCAACA GAGAAGGAAC AGAAATTTCC CACGTCCTGC TTTGCAATGT AACCCAGAGA 
GTGTCATTTT GGTTTGTGGT CACAGACCCT TTGAAAAACC ATACTTTTCC TGCAGTTGAA 
GTACAGTCAG CCATAAGAAT GAACAGGAAA CGGATCAACA GTGCATTCTT TTTGGATGAT 
CATACTCTGG AATTTTTAAA AATTCCTTCC ACTCTTGCTC CCCCGATGGA TCCATCTGTG 
CCCGTCTGGA TTATTGTATT TGGTGTGATA TTTTGCATTG TTACAGTTGC AATTGCACTA 
CTGGTTTTAT CCGGAATCCG GCAACGAAGA AGGAACAAGA AAGGACCACC TGGAGTGGAG 
GATGCAGAAG ACAAGTGTGA AAACATCATC ACAATTGAAA ATGGCATCCC TTGTGATCCC 
TTGGACATGA AGAGAGGGCA CATTAATGAT GGCTTCTTGA CAGAGGATGA GCGTCTCACC 
CCTCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Tmem27 dimerization, deglycosylation, plasma membrane depletion, and the extracellular Phe-Phe motif are negative regulators of cleavage by Bace2.
Biological chemistry393(6)473-84(2012 May)
Esterh?zy D,Akpinar P,Stoffel M

Age-related changes in the retinal pigment epithelium (RPE).
PloS one7(6)e38673(2012)
Gu X,Neric NJ,Crabb JS,Crabb JW,Bhattacharya SK,Rayborn ME,Hollyfield JG,Bonilha VL

Bace2 is a ? cell-enriched protease that regulates pancreatic ? cell function and mass.
Cell metabolism14(3)365-77(2011 Sep)
Esterh?zy D,St?tzer I,Wang H,Rechsteiner MP,Beauchamp J,D?beli H,Hilpert H,Matile H,Prummer M,Schmidt A,Lieske N,Boehm B,Marselli L,Bosco D,Kerr-Conte J,Aebersold R,Spinas GA,Moch H,Migliorini C,Stoffel M

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

Collectrin is involved in the development of salt-sensitive hypertension by facilitating the membrane trafficking of apical membrane proteins via interaction with soluble N-ethylmaleiamide-sensitive factor attachment protein receptor complex.
Circulation118(21)2146-55(2008 Nov)
Yasuhara A,Wada J,Malakauskas SM,Zhang Y,Eguchi J,Nakatsuka A,Murakami K,Kanzaki M,Teshigawara S,Yamagata K,Le TH,Makino H