Aadat cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aadat
Entrez Gene ID	23923
Full Name	aminoadipate aminotransferase
Synonyms	AI875679,Aadt,KATII,Kat2,Kyat2,mKat-2
General protein information	Preferred Names aminoadipate aminotransferase Names kynurenine/alpha-aminoadipate aminotransferase, mitochondrial 2-aminoadipate aminotransferase 2-aminoadipate transaminase KAT/AadAT alpha-aminoadipate aminotransferase kynurenine aminotransferase II kynurenine--oxoglutarate aminotransferase II kynurenine--oxoglutarate transaminase 2 kynurenine--oxoglutarate transaminase II
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8 B3.1\|8 30.85 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_011834.2	NP_035964.1	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
XM_006509397.3	XP_006509460.1	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform X1
XM_006509397.4	XP_006509460.1	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Aadat	NP_058889.1
Danio rerio (zebrafish)	LOC557979	XP_686234.6
Xenopus tropicalis (tropical clawed frog)	aadat	NP_001011226.1
Pan troglodytes (chimpanzee)	AADAT	XP_001155018.1
Canis lupus familiaris (dog)	AADAT	XP_543185.4
Magnaporthe oryzae (rice blast fungus)	MGG_14221	XP_003720489.1
Homo sapiens (human)	AADAT	NP_872603.1
Bos taurus (cattle)	LOC520638	XP_002689494.1
	AADAT	XP_002804303.1
Mus musculus (house mouse)	Aadat	NP_035964.1
Bos taurus (cattle)	AADAT	NP_001015551.1
Gallus gallus (chicken)	AADAT	XP_426286.3

Gene Ontology
Bibliography

Related articles in PubMed

Xanthurenic Acid Formation from 3-Hydroxykynurenine in the Mammalian Brain: Neurochemical Characterization and Physiological Effects.
Sathyasaikumar KV, Tararina M, Wu HQ, Neale SA, Weisz F, Salt TE, Schwarcz R
Neuroscience36785-97(2017 Dec)

Astrocytic and neuronal localization of kynurenine aminotransferase-2 in the adult mouse brain.
Her?di J, Berk? AM, Jankovics F, Iwamori T, Iwamori N, Ono E, Horv?th S, Kis Z, Toldi J, V?csei L, Gell?rt L
Brain structure & function222(4)1663-1672(2017 May)

Kynurenine aminotransferase III and glutamine transaminase L are identical enzymes that have cysteine S-conjugate ?-lyase activity and can transaminate L-selenomethionine.
Pinto JT, Krasnikov BF, Alcutt S, Jones ME, Dorai T, Villar MT, Artigues A, Li J, Cooper AJ
The Journal of biological chemistry289(45)30950-61(2014 Nov)

Reduction of endogenous kynurenic acid formation enhances extracellular glutamate, hippocampal plasticity, and cognitive behavior.
Potter MC, Elmer GI, Bergeron R, Albuquerque EX, Guidetti P, Wu HQ, Schwarcz R
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology35(8)1734-42(2010 Jul)

Thermal stability, pH dependence and inhibition of four murine kynurenine aminotransferases.
Han Q, Cai T, Tagle DA, Li J
BMC biochemistry1119(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

human KAT III/CCBL2 possesses cysteine S-conjugate beta-lyase activity, as does mouse KAT II
Title: Kynurenine aminotransferase III and glutamine transaminase L are identical enzymes that have cysteine S-conjugate ?-lyase activity and can transaminate L-selenomethionine.

This study showed that KAT II KO mice show the expected decrease in extracellular kynurenic acid levels, and that this reduction is accompanied by a significant increase in extracellular glutamate.
Title: Reduction of endogenous kynurenic acid formation enhances extracellular glutamate, hippocampal plasticity, and cognitive behavior.

The activity of mouse KAT II was not significantly inhibited by any proteinogenic amino acids at equimolar concentrations and half-life was 27.4 minutes at 65 degree C.
Title: Thermal stability, pH dependence and inhibition of four murine kynurenine aminotransferases.

Results show that gene targeting of kynurenine aminotransferase II (KAT) in mice leads to early and transitory decreases in brain KAT activity and kynurenic acid levels with commensurate behavioral and neuropathological changes.
Title: Biochemical and phenotypic abnormalities in kynurenine aminotransferase II-deficient mice.

Alpha7* nAChR and NMDA receptor functions were studied in kynurenine aminotransferase II null mice(mKat-2-/- mice). At 21 postnatal days, mKat-2-/- mice had lower hippocampal kynurenic acid levels and more spontaneous locomotor activity than control mice
Title: Targeted deletion of the kynurenine aminotransferase ii gene reveals a critical role of endogenous kynurenic acid in the regulation of synaptic transmission via alpha7 nicotinic receptors in the hippocampus.

The following Aadat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aadat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16031	NM_011834.2 Latest version!	Mus musculus aminoadipate aminotransferase (Aadat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu40834	XM_006509397.4 Latest version!	Mus musculus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OMu40834	XM_006509397.3	Mus musculus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aadat ( NM_011834.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035964.1 Mus musculus aminoadipate aminotransferase (Aadat), mRNA.

Price & Availability↑

CloneID	OMu16031
Clone ID Related Accession (Same CDS sequence)	NM_011834.2
Accession Version	NM_011834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC137514.2. On Nov 17, 2006 this sequence version replaced NM_011834.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF072376.1, AK075578.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385, SAMN00849386 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGAATTACT CACGGTTCCT CACTGCAACG AGCCTGGCCA GAAAGCCATC TCCCATCAGA 
ACTACAGCGG ACATACTGAG CAAAGCACCA AAAACCCTCA TCTCCCTGGC TCCTGGATCT 
CCAAACCCGA GCATGTTCCC CTTTAAGTCA GCTGCCTTCA CTGTGGAAAA CGGAAGCACC 
ATCCGGTTTG AAGACGACTT GATCAAAAGG GCCCTCCAAT ACTCTCCAAG CTATGGAATT 
CCAGAACTTC TGTCCTGGCT AAAACAGTTT CAAGTAAAAT TGCATAATCC CCCCACTGTC 
AACTACCCAC CCAATCAAGG ACAGATGGAT CTCTGCATCA CATCTGGCTG CCAAGATGGT 
CTCTGTAAGG CATTTGAAAT GCTCATCAAT CCTGGAGACA CTATCCTGGT CAATGAACCA 
CTGTTTCCAG GAACCCTTTA TGCTATGAAA CCACTAGGCT GCAACATTAT TAATGTCCCC 
AGTGATGAGC ATGGGATTAT TCCAGAGGGT CTCAAAAAAA TCCTTTCCCA GTGGAAACCA 
GAAGATTCCA AGGATCCCAC AAAAAAGACT CCAAAATTTC TGTATACTGT ACCAAATGGC 
AACAACCCTA CAGGCAACTC CTTGACAGGT GACCGCAAGA AGGAAATCTA TGAGCTTGCA 
AGAAAATATG ATTTCCTCAT AATAGAAGAT GACCCTTACT ATTTTCTTCA GTTTAGCAAG 
CCTTGGGAGC CAACTTTTCT CTCCATGGAT GTTGATGGGA GAGTCATCAG AGCTGACACC 
TTTTCAAAAA CCGTCTCCTC AGGGTTGAGA GTAGGGTTTA TGACTGGCCC TAAGACCTTG 
ATACAGAATA TTGTTCTCCA CACACAAGTC TCATCAGTAC ATGCCTGTAC TCTCTCACAG 
CTCATGATAC TACAGCTTCT ACACCAATGG GGAGAAGAGG GTTTCCTGGC TCATATTGAC 
AGAACTATTG ATTTCTACAA GAACCAGAGG GATTCCATAT TGGCAGCTGC AGACAAGTGG 
TTACGTGGCT TGGCAGAGTG GCATGTTCCC AAAGCTGGCA TGTTTCTATG GATTAAAGTT 
AAGGGAATCT CCGATACAAA ACAACTGATT GAAGAAAAGG CTATTGAAAG AGAGGTCTTA 
CTTGTTCCTG GAAATGGTTT CTTCATTGAT GGTTCAGCTC CTACCTCCTT CTTCAGAGCA 
TCCTTCTCTC TGGCTACTCC AGCACAGATG GATACAGCCT TCCAGAGATT GGCCCAACTG 
ATAAAAGAGT CTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035964.1
CDS	90..1367
Translation	MNYSRFLTATSLARKPSPIRTTADILSKAPKTLISLAPGSPNPSMFPFKSAAFTVENGSTIRFEDDLIKRALQYSPSYGIPELLSWLKQFQVKLHNPPTVNYPPNQGQMDLCITSGCQDGLCKAFEMLINPGDTILVNEPLFPGTLYAMKPLGCNIINVPSDEHGIIPEGLKKILSQWKPEDSKDPTKKTPKFLYTVPNGNNPTGNSLTGDRKKEIYELARKYDFLIIEDDPYYFLQFSKPWEPTFLSMDVDGRVIRADTFSKTVSSGLRVGFMTGPKTLIQNIVLHTQVSSVHACTLSQLMILQLLHQWGEEGFLAHIDRTIDFYKNQRDSILAAADKWLRGLAEWHVPKAGMFLWIKVKGISDTKQLIEEKAIEREVLLVPGNGFFIDGSAPTSFFRASFSLATPAQMDTAFQRLAQLIKESL

Target ORF information:

RefSeq Version	NM_011834.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminoadipate aminotransferase (Aadat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_011834.2

ORF Insert Sequence:

ATGAATTACT CACGGTTCCT CACTGCAACG AGCCTGGCCA GAAAGCCATC TCCCATCAGA 
ACTACAGCGG ACATACTGAG CAAAGCACCA AAAACCCTCA TCTCCCTGGC TCCTGGATCT 
CCAAACCCGA GCATGTTCCC CTTTAAGTCA GCTGCCTTCA CTGTGGAAAA CGGAAGCACC 
ATCCGGTTTG AAGACGACTT GATCAAAAGG GCCCTCCAAT ACTCTCCAAG CTATGGAATT 
CCAGAACTTC TGTCCTGGCT AAAACAGTTT CAAGTAAAAT TGCATAATCC CCCCACTGTC 
AACTACCCAC CCAATCAAGG ACAGATGGAT CTCTGCATCA CATCTGGCTG CCAAGATGGT 
CTCTGTAAGG CATTTGAAAT GCTCATCAAT CCTGGAGACA CTATCCTGGT CAATGAACCA 
CTGTTTCCAG GAACCCTTTA TGCTATGAAA CCACTAGGCT GCAACATTAT TAATGTCCCC 
AGTGATGAGC ATGGGATTAT TCCAGAGGGT CTCAAAAAAA TCCTTTCCCA GTGGAAACCA 
GAAGATTCCA AGGATCCCAC AAAAAAGACT CCAAAATTTC TGTATACTGT ACCAAATGGC 
AACAACCCTA CAGGCAACTC CTTGACAGGT GACCGCAAGA AGGAAATCTA TGAGCTTGCA 
AGAAAATATG ATTTCCTCAT AATAGAAGAT GACCCTTACT ATTTTCTTCA GTTTAGCAAG 
CCTTGGGAGC CAACTTTTCT CTCCATGGAT GTTGATGGGA GAGTCATCAG AGCTGACACC 
TTTTCAAAAA CCGTCTCCTC AGGGTTGAGA GTAGGGTTTA TGACTGGCCC TAAGACCTTG 
ATACAGAATA TTGTTCTCCA CACACAAGTC TCATCAGTAC ATGCCTGTAC TCTCTCACAG 
CTCATGATAC TACAGCTTCT ACACCAATGG GGAGAAGAGG GTTTCCTGGC TCATATTGAC 
AGAACTATTG ATTTCTACAA GAACCAGAGG GATTCCATAT TGGCAGCTGC AGACAAGTGG 
TTACGTGGCT TGGCAGAGTG GCATGTTCCC AAAGCTGGCA TGTTTCTATG GATTAAAGTT 
AAGGGAATCT CCGATACAAA ACAACTGATT GAAGAAAAGG CTATTGAAAG AGAGGTCTTA 
CTTGTTCCTG GAAATGGTTT CTTCATTGAT GGTTCAGCTC CTACCTCCTT CTTCAGAGCA 
TCCTTCTCTC TGGCTACTCC AGCACAGATG GATACAGCCT TCCAGAGATT GGCCCAACTG 
ATAAAAGAGT CTTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aadat ( XM_006509397.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509460.1 Mus musculus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40834
Clone ID Related Accession (Same CDS sequence)	XM_006509397.3 , XM_006509397.4
Accession Version	XM_006509397.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006509397.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACT CACGGTTCCT CACTGCAACG AGCCTGGCCA GAAAGCCATC TCCCATCAGA 
ACTACAGCGG ACATACTGAG CAAAGCACCA AAAACCCTCA TCTCCCTGGC TCCTGGATCT 
CCAAACCCGA GCATGTTCCC CTTTAAGTCA GCTGCCTTCA CTGTGGAAAA CGGAAGCACC 
ATCCGGTTTG AAGACGACTT GATCAAAAGG GCCCTCCAAT ACTCTCCAAG CTATGGAATT 
CCAGAACTTC TGTCCTGGCT AAAACAGTTT CAAGTAAAAT TGCATAATCC CCCCACTGTC 
AACTACCCAC CCAATCAAGG ACAGATGGAT CTCTGCATCA CATCTGGCTG CCAAGATGGT 
CTCTGTAAGG CATTTGAAAT GCTCATCAAT CCTGGAGACA CTATCCTGGT CAATGAACCA 
CTGTTTCCAG GAACCCTTTA TGCTATGAAA CCACTAGGCT GCAACATTAT TAATGTCCCC 
AGTGATGAGC ATGGGATTAT TCCAGAGGGT CTCAAAAAAA TCCTTTCCCA GTGGAAACCA 
GAAGATTCCA AGGATCCCAC AAAAAAGACT CCAAAATTTC TGTATACTGT ACCAAATGGC 
AACAACCCTA CAGGCAACTC CTTGACAGGT GACCGCAAGA AGGAAATCTA TGAGCTTGCA 
AGAAAATATG ATTTCCTCAT AATAGAAGAT GACCCTTACT ATTTTCTTCA GTTTAGCAAG 
CCTTGGGAGC CAACTTTTCT CTCCATGGAT GTTGATGGGA GAGTCATCAG AGCTGACACC 
TTTTCAAAAA CCGTCTCCTC AGGGTTGAGA GTAGGGTTTA TGACTGGCCC TAAGACCTTG 
ATACAGAATA TTGTTCTCCA CACACAAGTC TCATCAGTAC ATGCCTGTAC TCTCTCACAG 
CTCATGATAC TACAGCTTCT ACACCAATGG GGAGAAGAGG GTTTCCTGGC TCATATTGAC 
AGAACTATTG ATTTCTACAA GAACCAGAGG GATTCCATAT TGGCAGCTGC AGACAAGTGG 
TTACGTGGCT TGGCAGAGTG GCATGTTCCC AAAGCTGGCA TGTTTCTATG GATTAAAGTT 
AAGGGAATCT CCGATACAAA ACAACTGATT GAAGAAAAGG CTATTGAAAG AGAGGTCTTA 
CTTGTTCCTG GAAATGGTTT CTTCATTGAT GGTTCAGCTC CTACCTCCTT CTTCAGAGCA 
TCCTTCTCTC TGGCTACTCC AGCACAGATG GATACAGTGA GTATCCTCCT CCTACCTCCT 
TCAGAGCATC CTTCTCTCTG GCTACTTCAG CACAGATGGA TACAGCCTTC CAGAGATTGG 
CCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509460.1
CDS	371..1699
Translation	MNYSRFLTATSLARKPSPIRTTADILSKAPKTLISLAPGSPNPSMFPFKSAAFTVENGSTIRFEDDLIKRALQYSPSYGIPELLSWLKQFQVKLHNPPTVNYPPNQGQMDLCITSGCQDGLCKAFEMLINPGDTILVNEPLFPGTLYAMKPLGCNIINVPSDEHGIIPEGLKKILSQWKPEDSKDPTKKTPKFLYTVPNGNNPTGNSLTGDRKKEIYELARKYDFLIIEDDPYYFLQFSKPWEPTFLSMDVDGRVIRADTFSKTVSSGLRVGFMTGPKTLIQNIVLHTQVSSVHACTLSQLMILQLLHQWGEEGFLAHIDRTIDFYKNQRDSILAAADKWLRGLAEWHVPKAGMFLWIKVKGISDTKQLIEEKAIEREVLLVPGNGFFIDGSAPTSFFRASFSLATPAQMDTVSILLLPPSEHPSLWLLQHRWIQPSRDWPN

Target ORF information:

RefSeq Version	XM_006509397.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509397.4

ORF Insert Sequence:

ATGAATTACT CACGGTTCCT CACTGCAACG AGCCTGGCCA GAAAGCCATC TCCCATCAGA 
ACTACAGCGG ACATACTGAG CAAAGCACCA AAAACCCTCA TCTCCCTGGC TCCTGGATCT 
CCAAACCCGA GCATGTTCCC CTTTAAGTCA GCTGCCTTCA CTGTGGAAAA CGGAAGCACC 
ATCCGGTTTG AAGACGACTT GATCAAAAGG GCCCTCCAAT ACTCTCCAAG CTATGGAATT 
CCAGAACTTC TGTCCTGGCT AAAACAGTTT CAAGTAAAAT TGCATAATCC CCCCACTGTC 
AACTACCCAC CCAATCAAGG ACAGATGGAT CTCTGCATCA CATCTGGCTG CCAAGATGGT 
CTCTGTAAGG CATTTGAAAT GCTCATCAAT CCTGGAGACA CTATCCTGGT CAATGAACCA 
CTGTTTCCAG GAACCCTTTA TGCTATGAAA CCACTAGGCT GCAACATTAT TAATGTCCCC 
AGTGATGAGC ATGGGATTAT TCCAGAGGGT CTCAAAAAAA TCCTTTCCCA GTGGAAACCA 
GAAGATTCCA AGGATCCCAC AAAAAAGACT CCAAAATTTC TGTATACTGT ACCAAATGGC 
AACAACCCTA CAGGCAACTC CTTGACAGGT GACCGCAAGA AGGAAATCTA TGAGCTTGCA 
AGAAAATATG ATTTCCTCAT AATAGAAGAT GACCCTTACT ATTTTCTTCA GTTTAGCAAG 
CCTTGGGAGC CAACTTTTCT CTCCATGGAT GTTGATGGGA GAGTCATCAG AGCTGACACC 
TTTTCAAAAA CCGTCTCCTC AGGGTTGAGA GTAGGGTTTA TGACTGGCCC TAAGACCTTG 
ATACAGAATA TTGTTCTCCA CACACAAGTC TCATCAGTAC ATGCCTGTAC TCTCTCACAG 
CTCATGATAC TACAGCTTCT ACACCAATGG GGAGAAGAGG GTTTCCTGGC TCATATTGAC 
AGAACTATTG ATTTCTACAA GAACCAGAGG GATTCCATAT TGGCAGCTGC AGACAAGTGG 
TTACGTGGCT TGGCAGAGTG GCATGTTCCC AAAGCTGGCA TGTTTCTATG GATTAAAGTT 
AAGGGAATCT CCGATACAAA ACAACTGATT GAAGAAAAGG CTATTGAAAG AGAGGTCTTA 
CTTGTTCCTG GAAATGGTTT CTTCATTGAT GGTTCAGCTC CTACCTCCTT CTTCAGAGCA 
TCCTTCTCTC TGGCTACTCC AGCACAGATG GATACAGTGA GTATCCTCCT CCTACCTCCT 
TCAGAGCATC CTTCTCTCTG GCTACTTCAG CACAGATGGA TACAGCCTTC CAGAGATTGG 
CCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aadat ( XM_006509397.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006509460.1 Mus musculus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu40834
Clone ID Related Accession (Same CDS sequence)	XM_006509397.3 , XM_006509397.4
Accession Version	XM_006509397.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000074.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006509397.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACT CACGGTTCCT CACTGCAACG AGCCTGGCCA GAAAGCCATC TCCCATCAGA 
ACTACAGCGG ACATACTGAG CAAAGCACCA AAAACCCTCA TCTCCCTGGC TCCTGGATCT 
CCAAACCCGA GCATGTTCCC CTTTAAGTCA GCTGCCTTCA CTGTGGAAAA CGGAAGCACC 
ATCCGGTTTG AAGACGACTT GATCAAAAGG GCCCTCCAAT ACTCTCCAAG CTATGGAATT 
CCAGAACTTC TGTCCTGGCT AAAACAGTTT CAAGTAAAAT TGCATAATCC CCCCACTGTC 
AACTACCCAC CCAATCAAGG ACAGATGGAT CTCTGCATCA CATCTGGCTG CCAAGATGGT 
CTCTGTAAGG CATTTGAAAT GCTCATCAAT CCTGGAGACA CTATCCTGGT CAATGAACCA 
CTGTTTCCAG GAACCCTTTA TGCTATGAAA CCACTAGGCT GCAACATTAT TAATGTCCCC 
AGTGATGAGC ATGGGATTAT TCCAGAGGGT CTCAAAAAAA TCCTTTCCCA GTGGAAACCA 
GAAGATTCCA AGGATCCCAC AAAAAAGACT CCAAAATTTC TGTATACTGT ACCAAATGGC 
AACAACCCTA CAGGCAACTC CTTGACAGGT GACCGCAAGA AGGAAATCTA TGAGCTTGCA 
AGAAAATATG ATTTCCTCAT AATAGAAGAT GACCCTTACT ATTTTCTTCA GTTTAGCAAG 
CCTTGGGAGC CAACTTTTCT CTCCATGGAT GTTGATGGGA GAGTCATCAG AGCTGACACC 
TTTTCAAAAA CCGTCTCCTC AGGGTTGAGA GTAGGGTTTA TGACTGGCCC TAAGACCTTG 
ATACAGAATA TTGTTCTCCA CACACAAGTC TCATCAGTAC ATGCCTGTAC TCTCTCACAG 
CTCATGATAC TACAGCTTCT ACACCAATGG GGAGAAGAGG GTTTCCTGGC TCATATTGAC 
AGAACTATTG ATTTCTACAA GAACCAGAGG GATTCCATAT TGGCAGCTGC AGACAAGTGG 
TTACGTGGCT TGGCAGAGTG GCATGTTCCC AAAGCTGGCA TGTTTCTATG GATTAAAGTT 
AAGGGAATCT CCGATACAAA ACAACTGATT GAAGAAAAGG CTATTGAAAG AGAGGTCTTA 
CTTGTTCCTG GAAATGGTTT CTTCATTGAT GGTTCAGCTC CTACCTCCTT CTTCAGAGCA 
TCCTTCTCTC TGGCTACTCC AGCACAGATG GATACAGTGA GTATCCTCCT CCTACCTCCT 
TCAGAGCATC CTTCTCTCTG GCTACTTCAG CACAGATGGA TACAGCCTTC CAGAGATTGG 
CCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006509460.1
CDS	346..1674
Translation	MNYSRFLTATSLARKPSPIRTTADILSKAPKTLISLAPGSPNPSMFPFKSAAFTVENGSTIRFEDDLIKRALQYSPSYGIPELLSWLKQFQVKLHNPPTVNYPPNQGQMDLCITSGCQDGLCKAFEMLINPGDTILVNEPLFPGTLYAMKPLGCNIINVPSDEHGIIPEGLKKILSQWKPEDSKDPTKKTPKFLYTVPNGNNPTGNSLTGDRKKEIYELARKYDFLIIEDDPYYFLQFSKPWEPTFLSMDVDGRVIRADTFSKTVSSGLRVGFMTGPKTLIQNIVLHTQVSSVHACTLSQLMILQLLHQWGEEGFLAHIDRTIDFYKNQRDSILAAADKWLRGLAEWHVPKAGMFLWIKVKGISDTKQLIEEKAIEREVLLVPGNGFFIDGSAPTSFFRASFSLATPAQMDTVSILLLPPSEHPSLWLLQHRWIQPSRDWPN

Target ORF information:

RefSeq Version	XM_006509397.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006509397.3

ORF Insert Sequence:

ATGAATTACT CACGGTTCCT CACTGCAACG AGCCTGGCCA GAAAGCCATC TCCCATCAGA 
ACTACAGCGG ACATACTGAG CAAAGCACCA AAAACCCTCA TCTCCCTGGC TCCTGGATCT 
CCAAACCCGA GCATGTTCCC CTTTAAGTCA GCTGCCTTCA CTGTGGAAAA CGGAAGCACC 
ATCCGGTTTG AAGACGACTT GATCAAAAGG GCCCTCCAAT ACTCTCCAAG CTATGGAATT 
CCAGAACTTC TGTCCTGGCT AAAACAGTTT CAAGTAAAAT TGCATAATCC CCCCACTGTC 
AACTACCCAC CCAATCAAGG ACAGATGGAT CTCTGCATCA CATCTGGCTG CCAAGATGGT 
CTCTGTAAGG CATTTGAAAT GCTCATCAAT CCTGGAGACA CTATCCTGGT CAATGAACCA 
CTGTTTCCAG GAACCCTTTA TGCTATGAAA CCACTAGGCT GCAACATTAT TAATGTCCCC 
AGTGATGAGC ATGGGATTAT TCCAGAGGGT CTCAAAAAAA TCCTTTCCCA GTGGAAACCA 
GAAGATTCCA AGGATCCCAC AAAAAAGACT CCAAAATTTC TGTATACTGT ACCAAATGGC 
AACAACCCTA CAGGCAACTC CTTGACAGGT GACCGCAAGA AGGAAATCTA TGAGCTTGCA 
AGAAAATATG ATTTCCTCAT AATAGAAGAT GACCCTTACT ATTTTCTTCA GTTTAGCAAG 
CCTTGGGAGC CAACTTTTCT CTCCATGGAT GTTGATGGGA GAGTCATCAG AGCTGACACC 
TTTTCAAAAA CCGTCTCCTC AGGGTTGAGA GTAGGGTTTA TGACTGGCCC TAAGACCTTG 
ATACAGAATA TTGTTCTCCA CACACAAGTC TCATCAGTAC ATGCCTGTAC TCTCTCACAG 
CTCATGATAC TACAGCTTCT ACACCAATGG GGAGAAGAGG GTTTCCTGGC TCATATTGAC 
AGAACTATTG ATTTCTACAA GAACCAGAGG GATTCCATAT TGGCAGCTGC AGACAAGTGG 
TTACGTGGCT TGGCAGAGTG GCATGTTCCC AAAGCTGGCA TGTTTCTATG GATTAAAGTT 
AAGGGAATCT CCGATACAAA ACAACTGATT GAAGAAAAGG CTATTGAAAG AGAGGTCTTA 
CTTGTTCCTG GAAATGGTTT CTTCATTGAT GGTTCAGCTC CTACCTCCTT CTTCAGAGCA 
TCCTTCTCTC TGGCTACTCC AGCACAGATG GATACAGTGA GTATCCTCCT CCTACCTCCT 
TCAGAGCATC CTTCTCTCTG GCTACTTCAG CACAGATGGA TACAGCCTTC CAGAGATTGG 
CCCAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Xanthurenic Acid Formation from 3-Hydroxykynurenine in the Mammalian Brain: Neurochemical Characterization and Physiological Effects.
Neuroscience36785-97(2017 Dec)
Sathyasaikumar KV,Tararina M,Wu HQ,Neale SA,Weisz F,Salt TE,Schwarcz R

Astrocytic and neuronal localization of kynurenine aminotransferase-2 in the adult mouse brain.
Brain structure & function222(4)1663-1672(2017 May)
Her?di J,Berk? AM,Jankovics F,Iwamori T,Iwamori N,Ono E,Horv?th S,Kis Z,Toldi J,V?csei L,Gell?rt L

Kynurenine aminotransferase III and glutamine transaminase L are identical enzymes that have cysteine S-conjugate ?-lyase activity and can transaminate L-selenomethionine.
The Journal of biological chemistry289(45)30950-61(2014 Nov)
Pinto JT,Krasnikov BF,Alcutt S,Jones ME,Dorai T,Villar MT,Artigues A,Li J,Cooper AJ

Reduction of endogenous kynurenic acid formation enhances extracellular glutamate, hippocampal plasticity, and cognitive behavior.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology35(8)1734-42(2010 Jul)
Potter MC,Elmer GI,Bergeron R,Albuquerque EX,Guidetti P,Wu HQ,Schwarcz R

Thermal stability, pH dependence and inhibition of four murine kynurenine aminotransferases.
BMC biochemistry1119(2010 May)
Han Q,Cai T,Tagle DA,Li J