Aadat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aadat
Entrez Gene ID	29416
Full Name	aminoadipate aminotransferase
Synonyms	Kat2
General protein information	Preferred Names aminoadipate aminotransferase Names kynurenine/alpha-aminoadipate aminotransferase, mitochondrial 2-aminoadipate aminotransferase 2-aminoadipate transaminase KAT/AadAT alpha-aminoadipate aminotransferase kynurenine aminotransferase 2 kynurenine aminotransferase II kynurenine--oxoglutarate aminotransferase II kynurenine--oxoglutarate transaminase 2 kynurenine--oxoglutarate transaminase II
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p12
Summary	endogenous modulator of glutamatergic neurotransmission with kynurenine aminotransferase (KAT) activity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006253067.3	XP_006253129.1	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform X1
NM_017193.1	NP_058889.1	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Aadat	NP_058889.1
Danio rerio (zebrafish)	LOC557979	XP_686234.6
Xenopus tropicalis (tropical clawed frog)	aadat	NP_001011226.1
Pan troglodytes (chimpanzee)	AADAT	XP_001155018.1
Canis lupus familiaris (dog)	AADAT	XP_543185.4
Magnaporthe oryzae (rice blast fungus)	MGG_14221	XP_003720489.1
Homo sapiens (human)	AADAT	NP_872603.1
Bos taurus (cattle)	LOC520638	XP_002689494.1
	AADAT	XP_002804303.1
Mus musculus (house mouse)	Aadat	NP_035964.1
Bos taurus (cattle)	AADAT	NP_001015551.1
Gallus gallus (chicken)	AADAT	XP_426286.3

Gene Ontology
Bibliography

Related articles in PubMed

Structure, expression, and function of kynurenine aminotransferases in human and rodent brains.
Han Q, Cai T, Tagle DA, Li J
Cellular and molecular life sciences : CMLS67(3)353-68(2010 Feb)

Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II.
Han Q, Cai T, Tagle DA, Robinson H, Li J
Bioscience reports28(4)205-15(2008 Aug)

Crystal structure of human kynurenine aminotransferase II.
Han Q, Robinson H, Li J
The Journal of biological chemistry283(6)3567-73(2008 Feb)

Crystal structure of human kynurenine aminotransferase II, a drug target for the treatment of schizophrenia.
Rossi F, Garavaglia S, Montalbano V, Walsh MA, Rizzi M
The Journal of biological chemistry283(6)3559-66(2008 Feb)

Astrocytic localization of kynurenine aminotransferase II in the rat brain visualized by immunocytochemistry.
Guidetti P, Hoffman GE, Melendez-Ferro M, Albuquerque EX, Schwarcz R
Glia55(1)78-92(2007 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found specific staining of KAT II-positive astrocyte-like cells throughout the adult rat brain. The presence of KAT II in astrocytes was confirmed by double fluorescence immunostaining with an antibody against the astrocyte-specific marker GFAP.
Title: Astrocytic localization of kynurenine aminotransferase II in the rat brain visualized by immunocytochemistry.

identification of presence in the permanent immature rat oligodendrocyte cell line OLN-93
Title: Demonstration of kynurenine aminotransferases I and II and characterization of kynurenic acid synthesis in oligodendrocyte cell line (OLN-93).

neuronal culture showed positive immunostaining for both kynurenic acid aminotransferase (KAT) isoforms I and II
Title: Demonstration of kynurenine aminotransferases I and II and characterization of kynurenic acid synthesis in cultured cerebral cortical neurons.

The following Aadat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aadat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12491	NM_017193.1 Latest version!	Rattus norvegicus aminoadipate aminotransferase (Aadat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa37328	XM_006253067.3 Latest version!	Rattus norvegicus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aadat ( NM_017193.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058889.1 Rattus norvegicus aminoadipate aminotransferase (Aadat), mRNA.

Price & Availability↑

CloneID	ORa12491
Clone ID Related Accession (Same CDS sequence)	NM_017193.1
Accession Version	NM_017193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from Z50144.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z50144.1, BC078864.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAATTACT CAAGGTTCCT CACTGCAACG AGCCTGGCCA GAAAGACATC CCCTATCAGA 
GCTACAGTGG AGATAATGAG TAGAGCACCC AAAGACATCA TCTCCCTGGC TCCTGGATCT 
CCGAACCCGA AAGTGTTCCC CTTTAAGTCA GCTGTCTTCA CTGTGGAGAA CGGAAGCACC 
ATCCGGTTTG AAGGAGAGAT GTTTCAAAGG GCCCTCCAAT ATTCCTCAAG CTATGGAATT 
CCAGAACTTC TGTCCTGGCT AAAACAGTTG CAAATAAAAT TGCATAATCC TCCGACTGTC 
AACTACTCAC CCAACGAAGG ACAGATGGAC CTCTGCATCA CATCTGGCTG TCAAGACGGT 
CTCTGTAAGG TGTTTGAAAT GCTCATCAAT CCTGGAGACA CTGTTCTGGT CAATGAACCA 
CTGTATTCAG GAGCCCTTTT TGCAATGAAA CCACTGGGCT GCAATTTTAT TAGTGTCCCC 
AGTGATGACT GTGGGATTAT TCCAGAGGGT CTCAAAAAAG TACTTTCCCA GTGGAAACCA 
GAAGATTCCA AGGATCCCAC AAAAAGGACT CCAAAATTTC TGTATACTAT TCCGAATGGC 
AACAACCCTA CAGGCAACTC GTTGACTGGT GACCGCAAGA AAGAAATCTA TGAGCTTGCA 
AGAAAATATG ACTTCCTCAT AATAGAAGAC GATCCTTACT ATTTTCTCCA GTTCACCAAG 
CCTTGGGAAC CAACCTTTCT CTCCATGGAT GTTGATGGGA GAGTTATCAG AGCTGACTCC 
CTTTCAAAAG TTATCTCCTC AGGGCTGAGA GTGGGGTTTA TAACTGGCCC CAAGTCCTTG 
ATACAGAGGA TTGTTCTCCA CACACAAATC TCATCACTGC ATCCCTGTAC TTTATCACAG 
CTCATGATAT CGGAGCTTCT ATACCAGTGG GGAGAAGAGG GTTTCCTGGC CCATGTTGAC 
AGAGCTATTG ATTTCTACAA GAACCAGAGG GATTTTATAT TGGCAGCTGC AGACAAGTGG 
TTACGTGGTT TGGCAGAGTG GCATGTTCCC AAAGCTGGCA TGTTTCTATG GATTAAAGTT 
AACGGAATCT CTGATGCAAA AAAACTAATT GAAGAAAAGG CTATTGAAAG AGAGATCTTG 
TTAGTTCCTG GAAATAGTTT CTTCGTCGAT AATTCAGCCC CCTCCTCCTT CTTCAGAGCA 
TCCTTCTCTC AGGTTACTCC AGCGCAGATG GACTTAGTCT TCCAGAGATT GGCCCAACTC 
ATAAAAGACG TTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058889.1
CDS	113..1390
Translation	MNYSRFLTATSLARKTSPIRATVEIMSRAPKDIISLAPGSPNPKVFPFKSAVFTVENGSTIRFEGEMFQRALQYSSSYGIPELLSWLKQLQIKLHNPPTVNYSPNEGQMDLCITSGCQDGLCKVFEMLINPGDTVLVNEPLYSGALFAMKPLGCNFISVPSDDCGIIPEGLKKVLSQWKPEDSKDPTKRTPKFLYTIPNGNNPTGNSLTGDRKKEIYELARKYDFLIIEDDPYYFLQFTKPWEPTFLSMDVDGRVIRADSLSKVISSGLRVGFITGPKSLIQRIVLHTQISSLHPCTLSQLMISELLYQWGEEGFLAHVDRAIDFYKNQRDFILAAADKWLRGLAEWHVPKAGMFLWIKVNGISDAKKLIEEKAIEREILLVPGNSFFVDNSAPSSFFRASFSQVTPAQMDLVFQRLAQLIKDVS

Target ORF information:

RefSeq Version	NM_017193.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminoadipate aminotransferase (Aadat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017193.1

ORF Insert Sequence:

ATGAATTACT CAAGGTTCCT CACTGCAACG AGCCTGGCCA GAAAGACATC CCCTATCAGA 
GCTACAGTGG AGATAATGAG TAGAGCACCC AAAGACATCA TCTCCCTGGC TCCTGGATCT 
CCGAACCCGA AAGTGTTCCC CTTTAAGTCA GCTGTCTTCA CTGTGGAGAA CGGAAGCACC 
ATCCGGTTTG AAGGAGAGAT GTTTCAAAGG GCCCTCCAAT ATTCCTCAAG CTATGGAATT 
CCAGAACTTC TGTCCTGGCT AAAACAGTTG CAAATAAAAT TGCATAATCC TCCGACTGTC 
AACTACTCAC CCAACGAAGG ACAGATGGAC CTCTGCATCA CATCTGGCTG TCAAGACGGT 
CTCTGTAAGG TGTTTGAAAT GCTCATCAAT CCTGGAGACA CTGTTCTGGT CAATGAACCA 
CTGTATTCAG GAGCCCTTTT TGCAATGAAA CCACTGGGCT GCAATTTTAT TAGTGTCCCC 
AGTGATGACT GTGGGATTAT TCCAGAGGGT CTCAAAAAAG TACTTTCCCA GTGGAAACCA 
GAAGATTCCA AGGATCCCAC AAAAAGGACT CCAAAATTTC TGTATACTAT TCCGAATGGC 
AACAACCCTA CAGGCAACTC GTTGACTGGT GACCGCAAGA AAGAAATCTA TGAGCTTGCA 
AGAAAATATG ACTTCCTCAT AATAGAAGAC GATCCTTACT ATTTTCTCCA GTTCACCAAG 
CCTTGGGAAC CAACCTTTCT CTCCATGGAT GTTGATGGGA GAGTTATCAG AGCTGACTCC 
CTTTCAAAAG TTATCTCCTC AGGGCTGAGA GTGGGGTTTA TAACTGGCCC CAAGTCCTTG 
ATACAGAGGA TTGTTCTCCA CACACAAATC TCATCACTGC ATCCCTGTAC TTTATCACAG 
CTCATGATAT CGGAGCTTCT ATACCAGTGG GGAGAAGAGG GTTTCCTGGC CCATGTTGAC 
AGAGCTATTG ATTTCTACAA GAACCAGAGG GATTTTATAT TGGCAGCTGC AGACAAGTGG 
TTACGTGGTT TGGCAGAGTG GCATGTTCCC AAAGCTGGCA TGTTTCTATG GATTAAAGTT 
AACGGAATCT CTGATGCAAA AAAACTAATT GAAGAAAAGG CTATTGAAAG AGAGATCTTG 
TTAGTTCCTG GAAATAGTTT CTTCGTCGAT AATTCAGCCC CCTCCTCCTT CTTCAGAGCA 
TCCTTCTCTC AGGTTACTCC AGCGCAGATG GACTTAGTCT TCCAGAGATT GGCCCAACTC 
ATAAAAGACG TTTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Aadat ( XM_006253067.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006253129.1 Rattus norvegicus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa37328
Clone ID Related Accession (Same CDS sequence)	XM_006253067.3
Accession Version	XM_006253067.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006253067.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCTGG GGGCCAAACA TGGGGTACAC CTTGCAAGAA AGGCTCTACA GGGAAGACAG 
GACTGGGGAA TGGGAGCCAG AGGTGGAGCA GTGGAGATAA TGAGTAGAGC ACCCAAAGAC 
ATCATCTCCC TGGCTCCTGG ATCTCCGAAC CCGAAAGTGT TCCCCTTTAA GTCAGCTGTC 
TTCACTGTGG AGAACGGAAG CACCATCCGG TTTGAAGGAG AGATGTTTCA AAGGGCCCTC 
CAATATTCCT CAAGCTATGG AATTCCAGAA CTTCTGTCCT GGCTAAAACA GTTGCAAATA 
AAATTGCATA ATCCTCCGAC TGTCAACTAC TCACCCAACG AAGGACAGAT GGACCTCTGC 
ATCACATCTG GCTGTCAAGA CGGTCTCTGT AAGGTGTTTG AAATGCTCAT CAATCCTGGA 
GACACTGTTC TGGTCAATGA ACCACTGTAT TCAGGAGCCC TTTTTGCAAT GAAACCACTG 
GGCTGCAATT TTATTAGTGT CCCCAGTGAT GACTGTGGGA TTATTCCAGA GGGTCTCAAA 
AAAGTACTTT CCCAGTGGAA ACCAGAAGAT TCCAAGGATC CCACAAAAAG GACTCCAAAA 
TTTCTGTATA CTATTCCGAA TGGCAACAAC CCTACAGGCA ACTCGTTGAC TGGTGACCGC 
AAGAAAGAAA TCTATGAGCT TGCAAGAAAA TATGACTTCC TCATAATAGA AGACGATCCT 
TACTATTTTC TCCAGTTCAC CAAGCCTTGG GAACCAACCT TTCTCTCCAT GGATGTTGAT 
GGGAGAGTTA TCAGAGCTGA CTCCCTTTCA AAAGTTATCT CCTCAGGGCT GAGAGTGGGG 
TTTATAACTG GCCCCAAGTC CTTGATACAG AGGATTGTTC TCCACACACA AATCTCATCA 
CTGCATCCCT GTACTTTATC ACAGCTCATG ATATCGGAGC TTCTATACCA GTGGGGAGAA 
GAGGGTTTCC TGGCCCATGT TGACAGAGCT ATTGATTTCT ACAAGAACCA GAGGGATTTT 
ATATTGGCAG CTGCAGACAA GTGGTTACGT GGTTTGGCAG AGTGGCATGT TCCCAAAGCT 
GGCATGTTTC TATGGATTAA AGTTAACGGA ATCTCTGATG CAAAAAAACT AATTGAAGAA 
AAGGCTATTG AAAGAGAGAT CTTGTTAGTT CCTGGAAATA GTTTCTTCGT CGATAATTCA 
GCCCCCTCCT CCTTCTTCAG AGCATCCTTC TCTCAGGTTA CTCCAGCGCA GATGGACTTA 
GTCTTCCAGA GATTGGCCCA ACTCATAAAA GACGTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006253129.1
CDS	170..1471
Translation	MSLGAKHGVHLARKALQGRQDWGMGARGGAVEIMSRAPKDIISLAPGSPNPKVFPFKSAVFTVENGSTIRFEGEMFQRALQYSSSYGIPELLSWLKQLQIKLHNPPTVNYSPNEGQMDLCITSGCQDGLCKVFEMLINPGDTVLVNEPLYSGALFAMKPLGCNFISVPSDDCGIIPEGLKKVLSQWKPEDSKDPTKRTPKFLYTIPNGNNPTGNSLTGDRKKEIYELARKYDFLIIEDDPYYFLQFTKPWEPTFLSMDVDGRVIRADSLSKVISSGLRVGFITGPKSLIQRIVLHTQISSLHPCTLSQLMISELLYQWGEEGFLAHVDRAIDFYKNQRDFILAAADKWLRGLAEWHVPKAGMFLWIKVNGISDAKKLIEEKAIEREILLVPGNSFFVDNSAPSSFFRASFSQVTPAQMDLVFQRLAQLIKDVS

Target ORF information:

RefSeq Version	XM_006253067.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aminoadipate aminotransferase (Aadat), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006253067.3

ORF Insert Sequence:

ATGAGCCTGG GGGCCAAACA TGGGGTACAC CTTGCAAGAA AGGCTCTACA GGGAAGACAG 
GACTGGGGAA TGGGAGCCAG AGGTGGAGCA GTGGAGATAA TGAGTAGAGC ACCCAAAGAC 
ATCATCTCCC TGGCTCCTGG ATCTCCGAAC CCGAAAGTGT TCCCCTTTAA GTCAGCTGTC 
TTCACTGTGG AGAACGGAAG CACCATCCGG TTTGAAGGAG AGATGTTTCA AAGGGCCCTC 
CAATATTCCT CAAGCTATGG AATTCCAGAA CTTCTGTCCT GGCTAAAACA GTTGCAAATA 
AAATTGCATA ATCCTCCGAC TGTCAACTAC TCACCCAACG AAGGACAGAT GGACCTCTGC 
ATCACATCTG GCTGTCAAGA CGGTCTCTGT AAGGTGTTTG AAATGCTCAT CAATCCTGGA 
GACACTGTTC TGGTCAATGA ACCACTGTAT TCAGGAGCCC TTTTTGCAAT GAAACCACTG 
GGCTGCAATT TTATTAGTGT CCCCAGTGAT GACTGTGGGA TTATTCCAGA GGGTCTCAAA 
AAAGTACTTT CCCAGTGGAA ACCAGAAGAT TCCAAGGATC CCACAAAAAG GACTCCAAAA 
TTTCTGTATA CTATTCCGAA TGGCAACAAC CCTACAGGCA ACTCGTTGAC TGGTGACCGC 
AAGAAAGAAA TCTATGAGCT TGCAAGAAAA TATGACTTCC TCATAATAGA AGACGATCCT 
TACTATTTTC TCCAGTTCAC CAAGCCTTGG GAACCAACCT TTCTCTCCAT GGATGTTGAT 
GGGAGAGTTA TCAGAGCTGA CTCCCTTTCA AAAGTTATCT CCTCAGGGCT GAGAGTGGGG 
TTTATAACTG GCCCCAAGTC CTTGATACAG AGGATTGTTC TCCACACACA AATCTCATCA 
CTGCATCCCT GTACTTTATC ACAGCTCATG ATATCGGAGC TTCTATACCA GTGGGGAGAA 
GAGGGTTTCC TGGCCCATGT TGACAGAGCT ATTGATTTCT ACAAGAACCA GAGGGATTTT 
ATATTGGCAG CTGCAGACAA GTGGTTACGT GGTTTGGCAG AGTGGCATGT TCCCAAAGCT 
GGCATGTTTC TATGGATTAA AGTTAACGGA ATCTCTGATG CAAAAAAACT AATTGAAGAA 
AAGGCTATTG AAAGAGAGAT CTTGTTAGTT CCTGGAAATA GTTTCTTCGT CGATAATTCA 
GCCCCCTCCT CCTTCTTCAG AGCATCCTTC TCTCAGGTTA CTCCAGCGCA GATGGACTTA 
GTCTTCCAGA GATTGGCCCA ACTCATAAAA GACGTTTCAT AA