LOC104922882 gene cDNA ORF clone, Larimichthys crocea(large yellow croaker)

mRNA	Protein	Name
XM_019272862.1	XP_019128407.1	histone-lysine N-methyltransferase EHMT1 isoform X4
XM_019272860.1	XP_019128405.1	histone-lysine N-methyltransferase EHMT1 isoform X2
XM_019272861.1	XP_019128406.1	histone-lysine N-methyltransferase EHMT1 isoform X3
XM_019272862.2	XP_019128407.1	histone-lysine N-methyltransferase EHMT1 isoform X4
XM_019272860.2	XP_019128405.2	histone-lysine N-methyltransferase EHMT1 isoform X2
XM_019272861.2	XP_019128406.2	histone-lysine N-methyltransferase EHMT1 isoform X3
XM_019272859.1	XP_019128404.1	histone-lysine N-methyltransferase EHMT1 isoform X1
XM_019272859.2	XP_019128404.2	histone-lysine N-methyltransferase EHMT1 isoform X1
XM_027278613.1	XP_027134414.1	histone-lysine N-methyltransferase EHMT1 isoform X5
XM_027278614.1	XP_027134415.1	histone-lysine N-methyltransferase EHMT1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLa106342	XM_019272859.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OLa106343	XM_019272860.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OLa106344	XM_019272861.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OLa106345	XM_019272862.2 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OLa219523	XM_019272859.2 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OLa219524	XM_019272860.2 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OLa219525	XM_019272861.2 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OLa219526	XM_027278613.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OLa219527	XM_027278614.1 Latest version!	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OLa106345	XM_019272862.1	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

LOC104922882 ( XM_019272859.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128404.1 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLa106342
Clone ID Related Accession (Same CDS sequence)	XM_019272859.1
Accession Version	XM_019272859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4104bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGC GGGGAAAGAA AAGGAAAAGA GGAACAATCT GTTCGGCACA TTCTTGCTCT 
CTCTCCCTCT CCTTCGCCCT CTCTCTCACT CACACACACA CATTCTCCCT CACACACTCC 
CTCTCTGACG CCTTCTTCTT CTCAATGGAG GCCATGAGAA GAAAGCAGCC CGCTGGTCTA 
GCCAAGGGCA GTGTGGATAA GGGTGCCTCC ACAAAAAAAG AGGAACTCAA TGCAGCAAGC 
AACGGAGAGG AGAAGCCAGA TGGGGACGAA GAGGTAGCAG CCAGACTGGC CTCCACTGCA 
GCAGAGGCAA TGCTAAACGG CACTGAATGT GACGACGCGA GGCACAAGAG CCCGACACAC 
GGCTCTGCAA CGGGCAGCAA GAAGACTTTA CTGTTGGTTA ACGAAAACGG CACGTCAGAC 
ACAGAGCCAC CGCACGGTTC TGTCACTGGA AGCAATGGGT TCATTCTCAC TAAGCAGCAG 
CAGGAAGATG GCTCTGCAGC GACGCCGGGT TTGGGAGTTT CCCCTCATAG GACTAACTGG 
TTGCCTTCGG GCTCACCAGC AGGGGGGAGC ACAGCCAAAC CCCTCCCTGC CTTGGCGTCT 
GGGTGTGCAC AGAGTTTAGG TGCACTAAGG ACTGACCCCA GCATGGGGAC TACATCGGGA 
CAAGGGACAC CAGACACTAA AAATGGCACA GCACCAGCTC CAACACCGGC CCCAGCACCC 
GTCACGATAC ACAGAGCGCG CAAGACCATG TCCAGACCTG CTGTCAGTGC GGCACAAAAG 
CTTCTTAACA GGGAATTAAG AGAAGCAAAG AGTGCCAAAA TGGAGAAGAC GGAGGCTCAT 
GTTGCACCCG ACGCCCAGAA GTCCTCGTCG CAGTCCTCCG CTCAGAACCA CCTACCTCAG 
AGTCCACCGG ATACGCCAGC TTCAGCACAA ACGGCTTCAT CAGCTCCACC AACGCCTCCA 
GCGCCTCCAC CACCACCCCT GGCTCCCGCA CCACACTCTG CTCCTCCAGC TCCGGCCAAA 
CTCCACCTAG GCTCTTACTC AGGAGGGTTG TCATCCCGTA AGAAGAAAAG GAGGATGGGA 
ACGTACAGTC TGGTTCCCAA GAAGAAAGCC AAAGTGCTCA GGCAGAGAAC TATGATGGAA 
ATGTATAAGG ATTTACGACA CTCCAAGAGT CCACAGACTA AAGAGGTATC AAACATAAAT 
GGGGAGAAGG TTGAAAATGC ATCAGAGGAG GAGGAGTCCG AGGAGGTGGA GTCGGAGGAA 
GAGGAGCGAC AACAGCCAGC GGAAGAACCT GTCAGCGTTG CCCAGGAAGC AAGATCTGAA 
CCCATCTCTC AGGAGGCGGG AGACGAGCAG GAGTCTGAGG AGTCTGGAGA AGAAGAGGGA 
GAGGAGGAGG GCACAGAGTC TGACTTGAGC ACAGAGTCCA GTCTGAAGAA AAAGTTGAAA 
AAGAAAACAA AAGCAGACAG CGCGTGGCTC AGGCCGTCCA GGAAACGCAA GAGGAGGCTA 
AAGGCCAAAG TCTTCTGCTC AGAACCAGAG GCGGTGGATC AACCTCAGGC CTCAGCCCAC 
ACCCCGGCAG GCGATAGGAA GGAGTACACG CAGATTCCCT CGCCTGAGTC GGTCAACCTC 
AACAAGCCCC AAGAGCCGAC GGTTTCCTCA CAGGTCAAAG ACTCTCTGGG GTCGGCCATT 
GAGGAGGCTC AGGAGCTTCC GCTCTGCAGC TGCCGTATGG AGACGCCCAA GAGTCGAGAG 
ATTCTCATCC TGGCGGACAG GAAGTGCATG GCCACAGAGA GCGTGGATGG ACAGCTGACC 
CGCTGCCAGG GTGCCATACT GAAACATGAA ATGATGCGTC CCTCCAACTC CGTTCAGCTG 
CTGGTTCTGT GCGAGGACCA CCGTAACGGC ATGGTGAAGC ACCAGTGCTG CCCCGGGTGT 
GGCTTCTTCT GCAGGGCTGG GACCTTCATG GAGTGCCAAC CGGACGTCAG CATCTCTCAC 
CGTTTCCATC GTGCTTGTGC CTCAATGCTG AAAGGTCAAA GCTTCTGTCC CCACTGCGGA 
GAGGAGGCCA GCAAGGCCAA GGAGGTCACC ATCGCCAAGG CCGACACCAC CTCCACTGTG 
CCTCCTGGGC TGACACACGG ACCTGCCACC ACCGGGGTCC CCGAGGGCCG AGCAGACACC 
ACCACTGGCA GTTCTTCTCG ACTGGCCGTG GGGGCTGAAG TCAGCGGGAA GGCCGACAGT 
TCGCTGCCCA TCCGTTCTGC ACATGGACTC GACACCCCTG CTGTTCCCGG GTCATCTAGG 
ACCGCACTGC TGCAGGCCGG GACAGGAACA ATGGACTCAC CCACAATCCC TCAAGGAACG 
CAGAGAGAGA CTCTAGAGAG CATCCTGGTA GCTCTCGACA CAGAGAAACC CAAGAAGCTA 
CGTTTTCACC CCAAACAACT TTACCTCTCA GCCAAACAGG GAGAACTCAA GAAGGTCTTG 
CTTATGTTGG TGGACGGTAT CGACCCAAAT TTTAAGATGG AATCTCAGAA CAAACGCACA 
CCTCTCCATG CCGCAGCTGA GGGAGGACAC AAAGACATCT GTCACATGCT CGTACAAGCT 
GGTGCGAACT TGGACATGTG TGATGAAGAT CAGCGGACGC CACTCATGGA GGCGTGTGAG 
AACAACCACA TGGAGACTGT CCTGTACCTG CTGAGAGCTG GAGCAAGCGC CACGCACAAG 
GATGTTGAAG GGTTCACGTG TCTCCACCTA GCGGCAAAGT CTGGTCATTA CAACATTGTC 
GAGCACCTTC TGTCCACAGG ATTGATTAAC ATAAACTGTC AGGACGATGG CGGTTGGACA 
GCCATGATCT GGGCGACAGA GTACAAGCAT GTGGACCAGG TGAAGCTACT TCTGTCCAAA 
GGAGCTGACA TCAGCATCAG GGACAAGGAA GAGAATATCT GCCTTCACTG GGCAGCATTC 
TCCGGCAGTG TGGACATCGC CGAGCTGCTC CTGAACGCCC GCTGTGATCT GCAGGTCGTC 
AACATCCATG GAGACTCTCC TCTGCACATC GCTGCCCGGG AGAATCGTCT GGACTGTGTC 
ACACTTTTTC TGTCCCGAGG GGCAGATGTG TTTCTGAAGA ACCGCGAGGG AGAAACTCCT 
CCAGACTGCT GCAGCCACAA CTCAAAGGCC TGGGCAGCCC TGCAGGCCCA CAGGAGGGAG 
AAGGATGCCA AGAACAGCAG GCTCAATCGA ACAGAGCAAA AAGTCCTTCA CAGTGACATC 
GCTCTGGGGC AGGAGCGTGT TCCCATTCCC TGTGTCAACT CTGTCGACAG TGAACCTTAT 
CCAGACGGCT ACAAATACAT CCCCGAAAAC TGTGTCACTT CCCCCATGAA CATCGACAGG 
AACATCACAC ACTTGCAGTA CTGCGTTTGT AAAGAAGACT GTTCAACAAG TATCTGCATG 
TGTGGACAAC TCAGTCTGCG CTGCTGGTAC GACAAGGCTG GTCGTCTTCT ACCTGAATTC 
TGCCGTGAGG AGCCTCCTCT CATCTTTGAA TGTAACCATG CATGCTCCTG TTGGAGGACC 
TGTAAGAACC GTGTAGTGCA AAACGGACTC AGGACGAGGC TTCAGCTCTT CAGGACCAAT 
AAGAAGGGCT GGGGTGTTCG TGCAGTACAA GACATACCAC GGGGGACCTT TATATGCGAG 
TACGTAGGTG AGATCATCTC GGAAGCCGAG GCAGAGATGA GGCAGAATGA CGCTTACCTG 
TTCAGTCTGG ATGATAAGCC TCAAGATCTG TACTGCATCG ATGCCCGTTT CTATGGAAAC 
ATCAGCCGCT TCCTTAATCA CATGTGCGAG CCCAACTTGT TTGCCTGTCG AGTGTTCACG 
ACACACCAGG ACCTCCGTTT CCCACACATT GCCTTTTTTG CCAGTGAAAA CATCACAGCT 
GGAGAGGAGC TTGGATTTAA CTATGGCGAC CACTTCTGGG AAGTCAAAAG CAAACTGTTC 
AACTGCGAAT GTGGCTCTTC TAAGTGCAAG TACTCGTCAG CAGCCATGGC ATCGCTGCAG 
GCGGATAGCA CACCAGAGGA CCGGCAGCAG CCCAGCGCGT CACCTGACAC CAGCTCTTCC 
AACAGTCCTT CCAGTCCTTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128404.1
CDS	26..4129
Translation	MCMRGKKRKRGTICSAHSCSLSLSFALSLTHTHTFSLTHSLSDAFFFSMEAMRRKQPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQEAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKVFCSEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_019272859.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272859.1

ORF Insert Sequence:

ATGTGCATGC GGGGAAAGAA AAGGAAAAGA GGAACAATCT GTTCGGCACA TTCTTGCTCT 
CTCTCCCTCT CCTTCGCCCT CTCTCTCACT CACACACACA CATTCTCCCT CACACACTCC 
CTCTCTGACG CCTTCTTCTT CTCAATGGAG GCCATGAGAA GAAAGCAGCC CGCTGGTCTA 
GCCAAGGGCA GTGTGGATAA GGGTGCCTCC ACAAAAAAAG AGGAACTCAA TGCAGCAAGC 
AACGGAGAGG AGAAGCCAGA TGGGGACGAA GAGGTAGCAG CCAGACTGGC CTCCACTGCA 
GCAGAGGCAA TGCTAAACGG CACTGAATGT GACGACGCGA GGCACAAGAG CCCGACACAC 
GGCTCTGCAA CGGGCAGCAA GAAGACTTTA CTGTTGGTTA ACGAAAACGG CACGTCAGAC 
ACAGAGCCAC CGCACGGTTC TGTCACTGGA AGCAATGGGT TCATTCTCAC TAAGCAGCAG 
CAGGAAGATG GCTCTGCAGC GACGCCGGGT TTGGGAGTTT CCCCTCATAG GACTAACTGG 
TTGCCTTCGG GCTCACCAGC AGGGGGGAGC ACAGCCAAAC CCCTCCCTGC CTTGGCGTCT 
GGGTGTGCAC AGAGTTTAGG TGCACTAAGG ACTGACCCCA GCATGGGGAC TACATCGGGA 
CAAGGGACAC CAGACACTAA AAATGGCACA GCACCAGCTC CAACACCGGC CCCAGCACCC 
GTCACGATAC ACAGAGCGCG CAAGACCATG TCCAGACCTG CTGTCAGTGC GGCACAAAAG 
CTTCTTAACA GGGAATTAAG AGAAGCAAAG AGTGCCAAAA TGGAGAAGAC GGAGGCTCAT 
GTTGCACCCG ACGCCCAGAA GTCCTCGTCG CAGTCCTCCG CTCAGAACCA CCTACCTCAG 
AGTCCACCGG ATACGCCAGC TTCAGCACAA ACGGCTTCAT CAGCTCCACC AACGCCTCCA 
GCGCCTCCAC CACCACCCCT GGCTCCCGCA CCACACTCTG CTCCTCCAGC TCCGGCCAAA 
CTCCACCTAG GCTCTTACTC AGGAGGGTTG TCATCCCGTA AGAAGAAAAG GAGGATGGGA 
ACGTACAGTC TGGTTCCCAA GAAGAAAGCC AAAGTGCTCA GGCAGAGAAC TATGATGGAA 
ATGTATAAGG ATTTACGACA CTCCAAGAGT CCACAGACTA AAGAGGTATC AAACATAAAT 
GGGGAGAAGG TTGAAAATGC ATCAGAGGAG GAGGAGTCCG AGGAGGTGGA GTCGGAGGAA 
GAGGAGCGAC AACAGCCAGC GGAAGAACCT GTCAGCGTTG CCCAGGAAGC AAGATCTGAA 
CCCATCTCTC AGGAGGCGGG AGACGAGCAG GAGTCTGAGG AGTCTGGAGA AGAAGAGGGA 
GAGGAGGAGG GCACAGAGTC TGACTTGAGC ACAGAGTCCA GTCTGAAGAA AAAGTTGAAA 
AAGAAAACAA AAGCAGACAG CGCGTGGCTC AGGCCGTCCA GGAAACGCAA GAGGAGGCTA 
AAGGCCAAAG TCTTCTGCTC AGAACCAGAG GCGGTGGATC AACCTCAGGC CTCAGCCCAC 
ACCCCGGCAG GCGATAGGAA GGAGTACACG CAGATTCCCT CGCCTGAGTC GGTCAACCTC 
AACAAGCCCC AAGAGCCGAC GGTTTCCTCA CAGGTCAAAG ACTCTCTGGG GTCGGCCATT 
GAGGAGGCTC AGGAGCTTCC GCTCTGCAGC TGCCGTATGG AGACGCCCAA GAGTCGAGAG 
ATTCTCATCC TGGCGGACAG GAAGTGCATG GCCACAGAGA GCGTGGATGG ACAGCTGACC 
CGCTGCCAGG GTGCCATACT GAAACATGAA ATGATGCGTC CCTCCAACTC CGTTCAGCTG 
CTGGTTCTGT GCGAGGACCA CCGTAACGGC ATGGTGAAGC ACCAGTGCTG CCCCGGGTGT 
GGCTTCTTCT GCAGGGCTGG GACCTTCATG GAGTGCCAAC CGGACGTCAG CATCTCTCAC 
CGTTTCCATC GTGCTTGTGC CTCAATGCTG AAAGGTCAAA GCTTCTGTCC CCACTGCGGA 
GAGGAGGCCA GCAAGGCCAA GGAGGTCACC ATCGCCAAGG CCGACACCAC CTCCACTGTG 
CCTCCTGGGC TGACACACGG ACCTGCCACC ACCGGGGTCC CCGAGGGCCG AGCAGACACC 
ACCACTGGCA GTTCTTCTCG ACTGGCCGTG GGGGCTGAAG TCAGCGGGAA GGCCGACAGT 
TCGCTGCCCA TCCGTTCTGC ACATGGACTC GACACCCCTG CTGTTCCCGG GTCATCTAGG 
ACCGCACTGC TGCAGGCCGG GACAGGAACA ATGGACTCAC CCACAATCCC TCAAGGAACG 
CAGAGAGAGA CTCTAGAGAG CATCCTGGTA GCTCTCGACA CAGAGAAACC CAAGAAGCTA 
CGTTTTCACC CCAAACAACT TTACCTCTCA GCCAAACAGG GAGAACTCAA GAAGGTCTTG 
CTTATGTTGG TGGACGGTAT CGACCCAAAT TTTAAGATGG AATCTCAGAA CAAACGCACA 
CCTCTCCATG CCGCAGCTGA GGGAGGACAC AAAGACATCT GTCACATGCT CGTACAAGCT 
GGTGCGAACT TGGACATGTG TGATGAAGAT CAGCGGACGC CACTCATGGA GGCGTGTGAG 
AACAACCACA TGGAGACTGT CCTGTACCTG CTGAGAGCTG GAGCAAGCGC CACGCACAAG 
GATGTTGAAG GGTTCACGTG TCTCCACCTA GCGGCAAAGT CTGGTCATTA CAACATTGTC 
GAGCACCTTC TGTCCACAGG ATTGATTAAC ATAAACTGTC AGGACGATGG CGGTTGGACA 
GCCATGATCT GGGCGACAGA GTACAAGCAT GTGGACCAGG TGAAGCTACT TCTGTCCAAA 
GGAGCTGACA TCAGCATCAG GGACAAGGAA GAGAATATCT GCCTTCACTG GGCAGCATTC 
TCCGGCAGTG TGGACATCGC CGAGCTGCTC CTGAACGCCC GCTGTGATCT GCAGGTCGTC 
AACATCCATG GAGACTCTCC TCTGCACATC GCTGCCCGGG AGAATCGTCT GGACTGTGTC 
ACACTTTTTC TGTCCCGAGG GGCAGATGTG TTTCTGAAGA ACCGCGAGGG AGAAACTCCT 
CCAGACTGCT GCAGCCACAA CTCAAAGGCC TGGGCAGCCC TGCAGGCCCA CAGGAGGGAG 
AAGGATGCCA AGAACAGCAG GCTCAATCGA ACAGAGCAAA AAGTCCTTCA CAGTGACATC 
GCTCTGGGGC AGGAGCGTGT TCCCATTCCC TGTGTCAACT CTGTCGACAG TGAACCTTAT 
CCAGACGGCT ACAAATACAT CCCCGAAAAC TGTGTCACTT CCCCCATGAA CATCGACAGG 
AACATCACAC ACTTGCAGTA CTGCGTTTGT AAAGAAGACT GTTCAACAAG TATCTGCATG 
TGTGGACAAC TCAGTCTGCG CTGCTGGTAC GACAAGGCTG GTCGTCTTCT ACCTGAATTC 
TGCCGTGAGG AGCCTCCTCT CATCTTTGAA TGTAACCATG CATGCTCCTG TTGGAGGACC 
TGTAAGAACC GTGTAGTGCA AAACGGACTC AGGACGAGGC TTCAGCTCTT CAGGACCAAT 
AAGAAGGGCT GGGGTGTTCG TGCAGTACAA GACATACCAC GGGGGACCTT TATATGCGAG 
TACGTAGGTG AGATCATCTC GGAAGCCGAG GCAGAGATGA GGCAGAATGA CGCTTACCTG 
TTCAGTCTGG ATGATAAGCC TCAAGATCTG TACTGCATCG ATGCCCGTTT CTATGGAAAC 
ATCAGCCGCT TCCTTAATCA CATGTGCGAG CCCAACTTGT TTGCCTGTCG AGTGTTCACG 
ACACACCAGG ACCTCCGTTT CCCACACATT GCCTTTTTTG CCAGTGAAAA CATCACAGCT 
GGAGAGGAGC TTGGATTTAA CTATGGCGAC CACTTCTGGG AAGTCAAAAG CAAACTGTTC 
AACTGCGAAT GTGGCTCTTC TAAGTGCAAG TACTCGTCAG CAGCCATGGC ATCGCTGCAG 
GCGGATAGCA CACCAGAGGA CCGGCAGCAG CCCAGCGCGT CACCTGACAC CAGCTCTTCC 
AACAGTCCTT CCAGTCCTTC TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_019272860.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128405.1 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLa106343
Clone ID Related Accession (Same CDS sequence)	XM_019272860.1
Accession Version	XM_019272860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGC GGGGAAAGAA AAGGAAAAGA GGAACAATCT GTTCGGCACA TTCTTGCTCT 
CTCTCCCTCT CCTTCGCCCT CTCTCTCACT CACACACACA CATTCTCCCT CACACACTCC 
CTCTCTGACG CCTTCTTCTT CTCAATGGAG GCCATGAGAA GAAAGCAGCC CGCTGGTCTA 
GCCAAGGGCA GTGTGGATAA GGGTGCCTCC ACAAAAAAAG AGGAACTCAA TGCAGCAAGC 
AACGGAGAGG AGAAGCCAGA TGGGGACGAA GAGGTAGCAG CCAGACTGGC CTCCACTGCA 
GCAGAGGCAA TGCTAAACGG CACTGAATGT GACGACGCGA GGCACAAGAG CCCGACACAC 
GGCTCTGCAA CGGGCAGCAA GAAGACTTTA CTGTTGGTTA ACGAAAACGG CACGTCAGAC 
ACAGAGCCAC CGCACGGTTC TGTCACTGGA AGCAATGGGT TCATTCTCAC TAAGCAGCAG 
CAGGAAGATG GCTCTGCAGC GACGCCGGGT TTGGGAGTTT CCCCTCATAG GACTAACTGG 
TTGCCTTCGG GCTCACCAGC AGGGGGGAGC ACAGCCAAAC CCCTCCCTGC CTTGGCGTCT 
GGGTGTGCAC AGAGTTTAGG TGCACTAAGG ACTGACCCCA GCATGGGGAC TACATCGGGA 
CAAGGGACAC CAGACACTAA AAATGGCACA GCACCAGCTC CAACACCGGC CCCAGCACCC 
GTCACGATAC ACAGAGCGCG CAAGACCATG TCCAGACCTG CTGTCAGTGC GGCACAAAAG 
CTTCTTAACA GGGAATTAAG AGAAGCAAAG AGTGCCAAAA TGGAGAAGAC GGAGGCTCAT 
GTTGCACCCG ACGCCCAGAA GTCCTCGTCG CAGTCCTCCG CTCAGAACCA CCTACCTCAG 
AGTCCACCGG ATACGCCAGC TTCAGCACAA ACGGCTTCAT CAGCTCCACC AACGCCTCCA 
GCGCCTCCAC CACCACCCCT GGCTCCCGCA CCACACTCTG CTCCTCCAGC TCCGGCCAAA 
CTCCACCTAG GCTCTTACTC AGGAGGGTTG TCATCCCGTA AGAAGAAAAG GAGGATGGGA 
ACGTACAGTC TGGTTCCCAA GAAGAAAGCC AAAGTGCTCA GGCAGAGAAC TATGATGGAA 
ATGTATAAGG ATTTACGACA CTCCAAGAGT CCACAGACTA AAGAGGTATC AAACATAAAT 
GGGGAGAAGG TTGAAAATGC ATCAGAGGAG GAGGAGTCCG AGGAGGTGGA GTCGGAGGAA 
GAGGAGCGAC AACAGCCAGC GGAAGAACCT GTCAGCGTTG CCCAGGAAGC AAGATCTGAA 
CCCATCTCTC AGGCGGGAGA CGAGCAGGAG TCTGAGGAGT CTGGAGAAGA AGAGGGAGAG 
GAGGAGGGCA CAGAGTCTGA CTTGAGCACA GAGTCCAGTC TGAAGAAAAA GTTGAAAAAG 
AAAACAAAAG CAGACAGCGC GTGGCTCAGG CCGTCCAGGA AACGCAAGAG GAGGCTAAAG 
GCCAAAGTCT TCTGCTCAGA ACCAGAGGCG GTGGATCAAC CTCAGGCCTC AGCCCACACC 
CCGGCAGGCG ATAGGAAGGA GTACACGCAG ATTCCCTCGC CTGAGTCGGT CAACCTCAAC 
AAGCCCCAAG AGCCGACGGT TTCCTCACAG GTCAAAGACT CTCTGGGGTC GGCCATTGAG 
GAGGCTCAGG AGCTTCCGCT CTGCAGCTGC CGTATGGAGA CGCCCAAGAG TCGAGAGATT 
CTCATCCTGG CGGACAGGAA GTGCATGGCC ACAGAGAGCG TGGATGGACA GCTGACCCGC 
TGCCAGGGTG CCATACTGAA ACATGAAATG ATGCGTCCCT CCAACTCCGT TCAGCTGCTG 
GTTCTGTGCG AGGACCACCG TAACGGCATG GTGAAGCACC AGTGCTGCCC CGGGTGTGGC 
TTCTTCTGCA GGGCTGGGAC CTTCATGGAG TGCCAACCGG ACGTCAGCAT CTCTCACCGT 
TTCCATCGTG CTTGTGCCTC AATGCTGAAA GGTCAAAGCT TCTGTCCCCA CTGCGGAGAG 
GAGGCCAGCA AGGCCAAGGA GGTCACCATC GCCAAGGCCG ACACCACCTC CACTGTGCCT 
CCTGGGCTGA CACACGGACC TGCCACCACC GGGGTCCCCG AGGGCCGAGC AGACACCACC 
ACTGGCAGTT CTTCTCGACT GGCCGTGGGG GCTGAAGTCA GCGGGAAGGC CGACAGTTCG 
CTGCCCATCC GTTCTGCACA TGGACTCGAC ACCCCTGCTG TTCCCGGGTC ATCTAGGACC 
GCACTGCTGC AGGCCGGGAC AGGAACAATG GACTCACCCA CAATCCCTCA AGGAACGCAG 
AGAGAGACTC TAGAGAGCAT CCTGGTAGCT CTCGACACAG AGAAACCCAA GAAGCTACGT 
TTTCACCCCA AACAACTTTA CCTCTCAGCC AAACAGGGAG AACTCAAGAA GGTCTTGCTT 
ATGTTGGTGG ACGGTATCGA CCCAAATTTT AAGATGGAAT CTCAGAACAA ACGCACACCT 
CTCCATGCCG CAGCTGAGGG AGGACACAAA GACATCTGTC ACATGCTCGT ACAAGCTGGT 
GCGAACTTGG ACATGTGTGA TGAAGATCAG CGGACGCCAC TCATGGAGGC GTGTGAGAAC 
AACCACATGG AGACTGTCCT GTACCTGCTG AGAGCTGGAG CAAGCGCCAC GCACAAGGAT 
GTTGAAGGGT TCACGTGTCT CCACCTAGCG GCAAAGTCTG GTCATTACAA CATTGTCGAG 
CACCTTCTGT CCACAGGATT GATTAACATA AACTGTCAGG ACGATGGCGG TTGGACAGCC 
ATGATCTGGG CGACAGAGTA CAAGCATGTG GACCAGGTGA AGCTACTTCT GTCCAAAGGA 
GCTGACATCA GCATCAGGGA CAAGGAAGAG AATATCTGCC TTCACTGGGC AGCATTCTCC 
GGCAGTGTGG ACATCGCCGA GCTGCTCCTG AACGCCCGCT GTGATCTGCA GGTCGTCAAC 
ATCCATGGAG ACTCTCCTCT GCACATCGCT GCCCGGGAGA ATCGTCTGGA CTGTGTCACA 
CTTTTTCTGT CCCGAGGGGC AGATGTGTTT CTGAAGAACC GCGAGGGAGA AACTCCTCCA 
GACTGCTGCA GCCACAACTC AAAGGCCTGG GCAGCCCTGC AGGCCCACAG GAGGGAGAAG 
GATGCCAAGA ACAGCAGGCT CAATCGAACA GAGCAAAAAG TCCTTCACAG TGACATCGCT 
CTGGGGCAGG AGCGTGTTCC CATTCCCTGT GTCAACTCTG TCGACAGTGA ACCTTATCCA 
GACGGCTACA AATACATCCC CGAAAACTGT GTCACTTCCC CCATGAACAT CGACAGGAAC 
ATCACACACT TGCAGTACTG CGTTTGTAAA GAAGACTGTT CAACAAGTAT CTGCATGTGT 
GGACAACTCA GTCTGCGCTG CTGGTACGAC AAGGCTGGTC GTCTTCTACC TGAATTCTGC 
CGTGAGGAGC CTCCTCTCAT CTTTGAATGT AACCATGCAT GCTCCTGTTG GAGGACCTGT 
AAGAACCGTG TAGTGCAAAA CGGACTCAGG ACGAGGCTTC AGCTCTTCAG GACCAATAAG 
AAGGGCTGGG GTGTTCGTGC AGTACAAGAC ATACCACGGG GGACCTTTAT ATGCGAGTAC 
GTAGGTGAGA TCATCTCGGA AGCCGAGGCA GAGATGAGGC AGAATGACGC TTACCTGTTC 
AGTCTGGATG ATAAGCCTCA AGATCTGTAC TGCATCGATG CCCGTTTCTA TGGAAACATC 
AGCCGCTTCC TTAATCACAT GTGCGAGCCC AACTTGTTTG CCTGTCGAGT GTTCACGACA 
CACCAGGACC TCCGTTTCCC ACACATTGCC TTTTTTGCCA GTGAAAACAT CACAGCTGGA 
GAGGAGCTTG GATTTAACTA TGGCGACCAC TTCTGGGAAG TCAAAAGCAA ACTGTTCAAC 
TGCGAATGTG GCTCTTCTAA GTGCAAGTAC TCGTCAGCAG CCATGGCATC GCTGCAGGCG 
GATAGCACAC CAGAGGACCG GCAGCAGCCC AGCGCGTCAC CTGACACCAG CTCTTCCAAC 
AGTCCTTCCA GTCCTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128405.1
CDS	26..4126
Translation	MCMRGKKRKRGTICSAHSCSLSLSFALSLTHTHTFSLTHSLSDAFFFSMEAMRRKQPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKVFCSEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_019272860.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272860.1

ORF Insert Sequence:

ATGTGCATGC GGGGAAAGAA AAGGAAAAGA GGAACAATCT GTTCGGCACA TTCTTGCTCT 
CTCTCCCTCT CCTTCGCCCT CTCTCTCACT CACACACACA CATTCTCCCT CACACACTCC 
CTCTCTGACG CCTTCTTCTT CTCAATGGAG GCCATGAGAA GAAAGCAGCC CGCTGGTCTA 
GCCAAGGGCA GTGTGGATAA GGGTGCCTCC ACAAAAAAAG AGGAACTCAA TGCAGCAAGC 
AACGGAGAGG AGAAGCCAGA TGGGGACGAA GAGGTAGCAG CCAGACTGGC CTCCACTGCA 
GCAGAGGCAA TGCTAAACGG CACTGAATGT GACGACGCGA GGCACAAGAG CCCGACACAC 
GGCTCTGCAA CGGGCAGCAA GAAGACTTTA CTGTTGGTTA ACGAAAACGG CACGTCAGAC 
ACAGAGCCAC CGCACGGTTC TGTCACTGGA AGCAATGGGT TCATTCTCAC TAAGCAGCAG 
CAGGAAGATG GCTCTGCAGC GACGCCGGGT TTGGGAGTTT CCCCTCATAG GACTAACTGG 
TTGCCTTCGG GCTCACCAGC AGGGGGGAGC ACAGCCAAAC CCCTCCCTGC CTTGGCGTCT 
GGGTGTGCAC AGAGTTTAGG TGCACTAAGG ACTGACCCCA GCATGGGGAC TACATCGGGA 
CAAGGGACAC CAGACACTAA AAATGGCACA GCACCAGCTC CAACACCGGC CCCAGCACCC 
GTCACGATAC ACAGAGCGCG CAAGACCATG TCCAGACCTG CTGTCAGTGC GGCACAAAAG 
CTTCTTAACA GGGAATTAAG AGAAGCAAAG AGTGCCAAAA TGGAGAAGAC GGAGGCTCAT 
GTTGCACCCG ACGCCCAGAA GTCCTCGTCG CAGTCCTCCG CTCAGAACCA CCTACCTCAG 
AGTCCACCGG ATACGCCAGC TTCAGCACAA ACGGCTTCAT CAGCTCCACC AACGCCTCCA 
GCGCCTCCAC CACCACCCCT GGCTCCCGCA CCACACTCTG CTCCTCCAGC TCCGGCCAAA 
CTCCACCTAG GCTCTTACTC AGGAGGGTTG TCATCCCGTA AGAAGAAAAG GAGGATGGGA 
ACGTACAGTC TGGTTCCCAA GAAGAAAGCC AAAGTGCTCA GGCAGAGAAC TATGATGGAA 
ATGTATAAGG ATTTACGACA CTCCAAGAGT CCACAGACTA AAGAGGTATC AAACATAAAT 
GGGGAGAAGG TTGAAAATGC ATCAGAGGAG GAGGAGTCCG AGGAGGTGGA GTCGGAGGAA 
GAGGAGCGAC AACAGCCAGC GGAAGAACCT GTCAGCGTTG CCCAGGAAGC AAGATCTGAA 
CCCATCTCTC AGGCGGGAGA CGAGCAGGAG TCTGAGGAGT CTGGAGAAGA AGAGGGAGAG 
GAGGAGGGCA CAGAGTCTGA CTTGAGCACA GAGTCCAGTC TGAAGAAAAA GTTGAAAAAG 
AAAACAAAAG CAGACAGCGC GTGGCTCAGG CCGTCCAGGA AACGCAAGAG GAGGCTAAAG 
GCCAAAGTCT TCTGCTCAGA ACCAGAGGCG GTGGATCAAC CTCAGGCCTC AGCCCACACC 
CCGGCAGGCG ATAGGAAGGA GTACACGCAG ATTCCCTCGC CTGAGTCGGT CAACCTCAAC 
AAGCCCCAAG AGCCGACGGT TTCCTCACAG GTCAAAGACT CTCTGGGGTC GGCCATTGAG 
GAGGCTCAGG AGCTTCCGCT CTGCAGCTGC CGTATGGAGA CGCCCAAGAG TCGAGAGATT 
CTCATCCTGG CGGACAGGAA GTGCATGGCC ACAGAGAGCG TGGATGGACA GCTGACCCGC 
TGCCAGGGTG CCATACTGAA ACATGAAATG ATGCGTCCCT CCAACTCCGT TCAGCTGCTG 
GTTCTGTGCG AGGACCACCG TAACGGCATG GTGAAGCACC AGTGCTGCCC CGGGTGTGGC 
TTCTTCTGCA GGGCTGGGAC CTTCATGGAG TGCCAACCGG ACGTCAGCAT CTCTCACCGT 
TTCCATCGTG CTTGTGCCTC AATGCTGAAA GGTCAAAGCT TCTGTCCCCA CTGCGGAGAG 
GAGGCCAGCA AGGCCAAGGA GGTCACCATC GCCAAGGCCG ACACCACCTC CACTGTGCCT 
CCTGGGCTGA CACACGGACC TGCCACCACC GGGGTCCCCG AGGGCCGAGC AGACACCACC 
ACTGGCAGTT CTTCTCGACT GGCCGTGGGG GCTGAAGTCA GCGGGAAGGC CGACAGTTCG 
CTGCCCATCC GTTCTGCACA TGGACTCGAC ACCCCTGCTG TTCCCGGGTC ATCTAGGACC 
GCACTGCTGC AGGCCGGGAC AGGAACAATG GACTCACCCA CAATCCCTCA AGGAACGCAG 
AGAGAGACTC TAGAGAGCAT CCTGGTAGCT CTCGACACAG AGAAACCCAA GAAGCTACGT 
TTTCACCCCA AACAACTTTA CCTCTCAGCC AAACAGGGAG AACTCAAGAA GGTCTTGCTT 
ATGTTGGTGG ACGGTATCGA CCCAAATTTT AAGATGGAAT CTCAGAACAA ACGCACACCT 
CTCCATGCCG CAGCTGAGGG AGGACACAAA GACATCTGTC ACATGCTCGT ACAAGCTGGT 
GCGAACTTGG ACATGTGTGA TGAAGATCAG CGGACGCCAC TCATGGAGGC GTGTGAGAAC 
AACCACATGG AGACTGTCCT GTACCTGCTG AGAGCTGGAG CAAGCGCCAC GCACAAGGAT 
GTTGAAGGGT TCACGTGTCT CCACCTAGCG GCAAAGTCTG GTCATTACAA CATTGTCGAG 
CACCTTCTGT CCACAGGATT GATTAACATA AACTGTCAGG ACGATGGCGG TTGGACAGCC 
ATGATCTGGG CGACAGAGTA CAAGCATGTG GACCAGGTGA AGCTACTTCT GTCCAAAGGA 
GCTGACATCA GCATCAGGGA CAAGGAAGAG AATATCTGCC TTCACTGGGC AGCATTCTCC 
GGCAGTGTGG ACATCGCCGA GCTGCTCCTG AACGCCCGCT GTGATCTGCA GGTCGTCAAC 
ATCCATGGAG ACTCTCCTCT GCACATCGCT GCCCGGGAGA ATCGTCTGGA CTGTGTCACA 
CTTTTTCTGT CCCGAGGGGC AGATGTGTTT CTGAAGAACC GCGAGGGAGA AACTCCTCCA 
GACTGCTGCA GCCACAACTC AAAGGCCTGG GCAGCCCTGC AGGCCCACAG GAGGGAGAAG 
GATGCCAAGA ACAGCAGGCT CAATCGAACA GAGCAAAAAG TCCTTCACAG TGACATCGCT 
CTGGGGCAGG AGCGTGTTCC CATTCCCTGT GTCAACTCTG TCGACAGTGA ACCTTATCCA 
GACGGCTACA AATACATCCC CGAAAACTGT GTCACTTCCC CCATGAACAT CGACAGGAAC 
ATCACACACT TGCAGTACTG CGTTTGTAAA GAAGACTGTT CAACAAGTAT CTGCATGTGT 
GGACAACTCA GTCTGCGCTG CTGGTACGAC AAGGCTGGTC GTCTTCTACC TGAATTCTGC 
CGTGAGGAGC CTCCTCTCAT CTTTGAATGT AACCATGCAT GCTCCTGTTG GAGGACCTGT 
AAGAACCGTG TAGTGCAAAA CGGACTCAGG ACGAGGCTTC AGCTCTTCAG GACCAATAAG 
AAGGGCTGGG GTGTTCGTGC AGTACAAGAC ATACCACGGG GGACCTTTAT ATGCGAGTAC 
GTAGGTGAGA TCATCTCGGA AGCCGAGGCA GAGATGAGGC AGAATGACGC TTACCTGTTC 
AGTCTGGATG ATAAGCCTCA AGATCTGTAC TGCATCGATG CCCGTTTCTA TGGAAACATC 
AGCCGCTTCC TTAATCACAT GTGCGAGCCC AACTTGTTTG CCTGTCGAGT GTTCACGACA 
CACCAGGACC TCCGTTTCCC ACACATTGCC TTTTTTGCCA GTGAAAACAT CACAGCTGGA 
GAGGAGCTTG GATTTAACTA TGGCGACCAC TTCTGGGAAG TCAAAAGCAA ACTGTTCAAC 
TGCGAATGTG GCTCTTCTAA GTGCAAGTAC TCGTCAGCAG CCATGGCATC GCTGCAGGCG 
GATAGCACAC CAGAGGACCG GCAGCAGCCC AGCGCGTCAC CTGACACCAG CTCTTCCAAC 
AGTCCTTCCA GTCCTTCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_019272861.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128406.1 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X3, mRNA.

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CloneID	OLa106344
Clone ID Related Accession (Same CDS sequence)	XM_019272861.1
Accession Version	XM_019272861.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCATGC GGGGAAAGAA AAGGAAAAGA GGAACAATCT GTTCGGCACA TTCTTGCTCT 
CTCTCCCTCT CCTTCGCCCT CTCTCTCACT CACACACACA CATTCTCCCT CACACACTCC 
CTCTCTGACG CCTTCTTCTT CTCAATGGAG GCCATGAGAA GAAAGCAGCC CGCTGGTCTA 
GCCAAGGGCA GTGTGGATAA GGGTGCCTCC ACAAAAAAAG AGGAACTCAA TGCAGCAAGC 
AACGGAGAGG AGAAGCCAGA TGGGGACGAA GAGGTAGCAG CCAGACTGGC CTCCACTGCA 
GCAGAGGCAA TGCTAAACGG CACTGAATGT GACGACGCGA GGCACAAGAG CCCGACACAC 
GGCTCTGCAA CGGGCAGCAA GAAGACTTTA CTGTTGGTTA ACGAAAACGG CACGTCAGAC 
ACAGAGCCAC CGCACGGTTC TGTCACTGGA AGCAATGGGT TCATTCTCAC TAAGCAGCAG 
CAGGAAGATG GCTCTGCAGC GACGCCGGGT TTGGGAGTTT CCCCTCATAG GACTAACTGG 
TTGCCTTCGG GCTCACCAGC AGGGGGGAGC ACAGCCAAAC CCCTCCCTGC CTTGGCGTCT 
GGGTGTGCAC AGAGTTTAGG TGCACTAAGG ACTGACCCCA GCATGGGGAC TACATCGGGA 
CAAGGGACAC CAGACACTAA AAATGGCACA GCACCAGCTC CAACACCGGC CCCAGCACCC 
GTCACGATAC ACAGAGCGCG CAAGACCATG TCCAGACCTG CTGTCAGTGC GGCACAAAAG 
CTTCTTAACA GGGAATTAAG AGAAGCAAAG AGTGCCAAAA TGGAGAAGAC GGAGGCTCAT 
GTTGCACCCG ACGCCCAGAA GTCCTCGTCG CAGTCCTCCG CTCAGAACCA CCTACCTCAG 
AGTCCACCGG ATACGCCAGC TTCAGCACAA ACGGCTTCAT CAGCTCCACC AACGCCTCCA 
GCGCCTCCAC CACCACCCCT GGCTCCCGCA CCACACTCTG CTCCTCCAGC TCCGGCCAAA 
CTCCACCTAG GCTCTTACTC AGGAGGGTTG TCATCCCGTA AGAAGAAAAG GAGGATGGGA 
ACGTACAGTC TGGTTCCCAA GAAGAAAGCC AAAGTGCTCA GGCAGAGAAC TATGATGGAA 
ATGTATAAGG ATTTACGACA CTCCAAGAGT CCACAGACTA AAGAGGTATC AAACATAAAT 
GGGGAGAAGG TTGAAAATGC ATCAGAGGAG GAGGAGTCCG AGGAGGTGGA GTCGGAGGAA 
GAGGAGCGAC AACAGCCAGC GGAAGAACCT GTCAGCGTTG CCCAGGAAGC AAGATCTGAA 
CCCATCTCTC AGGAGGCGGG AGACGAGCAG GAGTCTGAGG AGTCTGGAGA AGAAGAGGGA 
GAGGAGGAGG GCACAGAGTC TGACTTGAGC ACAGAGTCCA GTCTGAAGAA AAAGTTGAAA 
AAGAAAACAA AAGCAGACAG CGCGTGGCTC AGGCCGTCCA GGAAACGCAA GAGGAGGCTA 
AAGGCCAAAG AACCAGAGGC GGTGGATCAA CCTCAGGCCT CAGCCCACAC CCCGGCAGGC 
GATAGGAAGG AGTACACGCA GATTCCCTCG CCTGAGTCGG TCAACCTCAA CAAGCCCCAA 
GAGCCGACGG TTTCCTCACA GGTCAAAGAC TCTCTGGGGT CGGCCATTGA GGAGGCTCAG 
GAGCTTCCGC TCTGCAGCTG CCGTATGGAG ACGCCCAAGA GTCGAGAGAT TCTCATCCTG 
GCGGACAGGA AGTGCATGGC CACAGAGAGC GTGGATGGAC AGCTGACCCG CTGCCAGGGT 
GCCATACTGA AACATGAAAT GATGCGTCCC TCCAACTCCG TTCAGCTGCT GGTTCTGTGC 
GAGGACCACC GTAACGGCAT GGTGAAGCAC CAGTGCTGCC CCGGGTGTGG CTTCTTCTGC 
AGGGCTGGGA CCTTCATGGA GTGCCAACCG GACGTCAGCA TCTCTCACCG TTTCCATCGT 
GCTTGTGCCT CAATGCTGAA AGGTCAAAGC TTCTGTCCCC ACTGCGGAGA GGAGGCCAGC 
AAGGCCAAGG AGGTCACCAT CGCCAAGGCC GACACCACCT CCACTGTGCC TCCTGGGCTG 
ACACACGGAC CTGCCACCAC CGGGGTCCCC GAGGGCCGAG CAGACACCAC CACTGGCAGT 
TCTTCTCGAC TGGCCGTGGG GGCTGAAGTC AGCGGGAAGG CCGACAGTTC GCTGCCCATC 
CGTTCTGCAC ATGGACTCGA CACCCCTGCT GTTCCCGGGT CATCTAGGAC CGCACTGCTG 
CAGGCCGGGA CAGGAACAAT GGACTCACCC ACAATCCCTC AAGGAACGCA GAGAGAGACT 
CTAGAGAGCA TCCTGGTAGC TCTCGACACA GAGAAACCCA AGAAGCTACG TTTTCACCCC 
AAACAACTTT ACCTCTCAGC CAAACAGGGA GAACTCAAGA AGGTCTTGCT TATGTTGGTG 
GACGGTATCG ACCCAAATTT TAAGATGGAA TCTCAGAACA AACGCACACC TCTCCATGCC 
GCAGCTGAGG GAGGACACAA AGACATCTGT CACATGCTCG TACAAGCTGG TGCGAACTTG 
GACATGTGTG ATGAAGATCA GCGGACGCCA CTCATGGAGG CGTGTGAGAA CAACCACATG 
GAGACTGTCC TGTACCTGCT GAGAGCTGGA GCAAGCGCCA CGCACAAGGA TGTTGAAGGG 
TTCACGTGTC TCCACCTAGC GGCAAAGTCT GGTCATTACA ACATTGTCGA GCACCTTCTG 
TCCACAGGAT TGATTAACAT AAACTGTCAG GACGATGGCG GTTGGACAGC CATGATCTGG 
GCGACAGAGT ACAAGCATGT GGACCAGGTG AAGCTACTTC TGTCCAAAGG AGCTGACATC 
AGCATCAGGG ACAAGGAAGA GAATATCTGC CTTCACTGGG CAGCATTCTC CGGCAGTGTG 
GACATCGCCG AGCTGCTCCT GAACGCCCGC TGTGATCTGC AGGTCGTCAA CATCCATGGA 
GACTCTCCTC TGCACATCGC TGCCCGGGAG AATCGTCTGG ACTGTGTCAC ACTTTTTCTG 
TCCCGAGGGG CAGATGTGTT TCTGAAGAAC CGCGAGGGAG AAACTCCTCC AGACTGCTGC 
AGCCACAACT CAAAGGCCTG GGCAGCCCTG CAGGCCCACA GGAGGGAGAA GGATGCCAAG 
AACAGCAGGC TCAATCGAAC AGAGCAAAAA GTCCTTCACA GTGACATCGC TCTGGGGCAG 
GAGCGTGTTC CCATTCCCTG TGTCAACTCT GTCGACAGTG AACCTTATCC AGACGGCTAC 
AAATACATCC CCGAAAACTG TGTCACTTCC CCCATGAACA TCGACAGGAA CATCACACAC 
TTGCAGTACT GCGTTTGTAA AGAAGACTGT TCAACAAGTA TCTGCATGTG TGGACAACTC 
AGTCTGCGCT GCTGGTACGA CAAGGCTGGT CGTCTTCTAC CTGAATTCTG CCGTGAGGAG 
CCTCCTCTCA TCTTTGAATG TAACCATGCA TGCTCCTGTT GGAGGACCTG TAAGAACCGT 
GTAGTGCAAA ACGGACTCAG GACGAGGCTT CAGCTCTTCA GGACCAATAA GAAGGGCTGG 
GGTGTTCGTG CAGTACAAGA CATACCACGG GGGACCTTTA TATGCGAGTA CGTAGGTGAG 
ATCATCTCGG AAGCCGAGGC AGAGATGAGG CAGAATGACG CTTACCTGTT CAGTCTGGAT 
GATAAGCCTC AAGATCTGTA CTGCATCGAT GCCCGTTTCT ATGGAAACAT CAGCCGCTTC 
CTTAATCACA TGTGCGAGCC CAACTTGTTT GCCTGTCGAG TGTTCACGAC ACACCAGGAC 
CTCCGTTTCC CACACATTGC CTTTTTTGCC AGTGAAAACA TCACAGCTGG AGAGGAGCTT 
GGATTTAACT ATGGCGACCA CTTCTGGGAA GTCAAAAGCA AACTGTTCAA CTGCGAATGT 
GGCTCTTCTA AGTGCAAGTA CTCGTCAGCA GCCATGGCAT CGCTGCAGGC GGATAGCACA 
CCAGAGGACC GGCAGCAGCC CAGCGCGTCA CCTGACACCA GCTCTTCCAA CAGTCCTTCC 
AGTCCTTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128406.1
CDS	25..4116
Translation	MCMRGKKRKRGTICSAHSCSLSLSFALSLTHTHTFSLTHSLSDAFFFSMEAMRRKQPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQEAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_019272861.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272861.1

ORF Insert Sequence:

ATGTGCATGC GGGGAAAGAA AAGGAAAAGA GGAACAATCT GTTCGGCACA TTCTTGCTCT 
CTCTCCCTCT CCTTCGCCCT CTCTCTCACT CACACACACA CATTCTCCCT CACACACTCC 
CTCTCTGACG CCTTCTTCTT CTCAATGGAG GCCATGAGAA GAAAGCAGCC CGCTGGTCTA 
GCCAAGGGCA GTGTGGATAA GGGTGCCTCC ACAAAAAAAG AGGAACTCAA TGCAGCAAGC 
AACGGAGAGG AGAAGCCAGA TGGGGACGAA GAGGTAGCAG CCAGACTGGC CTCCACTGCA 
GCAGAGGCAA TGCTAAACGG CACTGAATGT GACGACGCGA GGCACAAGAG CCCGACACAC 
GGCTCTGCAA CGGGCAGCAA GAAGACTTTA CTGTTGGTTA ACGAAAACGG CACGTCAGAC 
ACAGAGCCAC CGCACGGTTC TGTCACTGGA AGCAATGGGT TCATTCTCAC TAAGCAGCAG 
CAGGAAGATG GCTCTGCAGC GACGCCGGGT TTGGGAGTTT CCCCTCATAG GACTAACTGG 
TTGCCTTCGG GCTCACCAGC AGGGGGGAGC ACAGCCAAAC CCCTCCCTGC CTTGGCGTCT 
GGGTGTGCAC AGAGTTTAGG TGCACTAAGG ACTGACCCCA GCATGGGGAC TACATCGGGA 
CAAGGGACAC CAGACACTAA AAATGGCACA GCACCAGCTC CAACACCGGC CCCAGCACCC 
GTCACGATAC ACAGAGCGCG CAAGACCATG TCCAGACCTG CTGTCAGTGC GGCACAAAAG 
CTTCTTAACA GGGAATTAAG AGAAGCAAAG AGTGCCAAAA TGGAGAAGAC GGAGGCTCAT 
GTTGCACCCG ACGCCCAGAA GTCCTCGTCG CAGTCCTCCG CTCAGAACCA CCTACCTCAG 
AGTCCACCGG ATACGCCAGC TTCAGCACAA ACGGCTTCAT CAGCTCCACC AACGCCTCCA 
GCGCCTCCAC CACCACCCCT GGCTCCCGCA CCACACTCTG CTCCTCCAGC TCCGGCCAAA 
CTCCACCTAG GCTCTTACTC AGGAGGGTTG TCATCCCGTA AGAAGAAAAG GAGGATGGGA 
ACGTACAGTC TGGTTCCCAA GAAGAAAGCC AAAGTGCTCA GGCAGAGAAC TATGATGGAA 
ATGTATAAGG ATTTACGACA CTCCAAGAGT CCACAGACTA AAGAGGTATC AAACATAAAT 
GGGGAGAAGG TTGAAAATGC ATCAGAGGAG GAGGAGTCCG AGGAGGTGGA GTCGGAGGAA 
GAGGAGCGAC AACAGCCAGC GGAAGAACCT GTCAGCGTTG CCCAGGAAGC AAGATCTGAA 
CCCATCTCTC AGGAGGCGGG AGACGAGCAG GAGTCTGAGG AGTCTGGAGA AGAAGAGGGA 
GAGGAGGAGG GCACAGAGTC TGACTTGAGC ACAGAGTCCA GTCTGAAGAA AAAGTTGAAA 
AAGAAAACAA AAGCAGACAG CGCGTGGCTC AGGCCGTCCA GGAAACGCAA GAGGAGGCTA 
AAGGCCAAAG AACCAGAGGC GGTGGATCAA CCTCAGGCCT CAGCCCACAC CCCGGCAGGC 
GATAGGAAGG AGTACACGCA GATTCCCTCG CCTGAGTCGG TCAACCTCAA CAAGCCCCAA 
GAGCCGACGG TTTCCTCACA GGTCAAAGAC TCTCTGGGGT CGGCCATTGA GGAGGCTCAG 
GAGCTTCCGC TCTGCAGCTG CCGTATGGAG ACGCCCAAGA GTCGAGAGAT TCTCATCCTG 
GCGGACAGGA AGTGCATGGC CACAGAGAGC GTGGATGGAC AGCTGACCCG CTGCCAGGGT 
GCCATACTGA AACATGAAAT GATGCGTCCC TCCAACTCCG TTCAGCTGCT GGTTCTGTGC 
GAGGACCACC GTAACGGCAT GGTGAAGCAC CAGTGCTGCC CCGGGTGTGG CTTCTTCTGC 
AGGGCTGGGA CCTTCATGGA GTGCCAACCG GACGTCAGCA TCTCTCACCG TTTCCATCGT 
GCTTGTGCCT CAATGCTGAA AGGTCAAAGC TTCTGTCCCC ACTGCGGAGA GGAGGCCAGC 
AAGGCCAAGG AGGTCACCAT CGCCAAGGCC GACACCACCT CCACTGTGCC TCCTGGGCTG 
ACACACGGAC CTGCCACCAC CGGGGTCCCC GAGGGCCGAG CAGACACCAC CACTGGCAGT 
TCTTCTCGAC TGGCCGTGGG GGCTGAAGTC AGCGGGAAGG CCGACAGTTC GCTGCCCATC 
CGTTCTGCAC ATGGACTCGA CACCCCTGCT GTTCCCGGGT CATCTAGGAC CGCACTGCTG 
CAGGCCGGGA CAGGAACAAT GGACTCACCC ACAATCCCTC AAGGAACGCA GAGAGAGACT 
CTAGAGAGCA TCCTGGTAGC TCTCGACACA GAGAAACCCA AGAAGCTACG TTTTCACCCC 
AAACAACTTT ACCTCTCAGC CAAACAGGGA GAACTCAAGA AGGTCTTGCT TATGTTGGTG 
GACGGTATCG ACCCAAATTT TAAGATGGAA TCTCAGAACA AACGCACACC TCTCCATGCC 
GCAGCTGAGG GAGGACACAA AGACATCTGT CACATGCTCG TACAAGCTGG TGCGAACTTG 
GACATGTGTG ATGAAGATCA GCGGACGCCA CTCATGGAGG CGTGTGAGAA CAACCACATG 
GAGACTGTCC TGTACCTGCT GAGAGCTGGA GCAAGCGCCA CGCACAAGGA TGTTGAAGGG 
TTCACGTGTC TCCACCTAGC GGCAAAGTCT GGTCATTACA ACATTGTCGA GCACCTTCTG 
TCCACAGGAT TGATTAACAT AAACTGTCAG GACGATGGCG GTTGGACAGC CATGATCTGG 
GCGACAGAGT ACAAGCATGT GGACCAGGTG AAGCTACTTC TGTCCAAAGG AGCTGACATC 
AGCATCAGGG ACAAGGAAGA GAATATCTGC CTTCACTGGG CAGCATTCTC CGGCAGTGTG 
GACATCGCCG AGCTGCTCCT GAACGCCCGC TGTGATCTGC AGGTCGTCAA CATCCATGGA 
GACTCTCCTC TGCACATCGC TGCCCGGGAG AATCGTCTGG ACTGTGTCAC ACTTTTTCTG 
TCCCGAGGGG CAGATGTGTT TCTGAAGAAC CGCGAGGGAG AAACTCCTCC AGACTGCTGC 
AGCCACAACT CAAAGGCCTG GGCAGCCCTG CAGGCCCACA GGAGGGAGAA GGATGCCAAG 
AACAGCAGGC TCAATCGAAC AGAGCAAAAA GTCCTTCACA GTGACATCGC TCTGGGGCAG 
GAGCGTGTTC CCATTCCCTG TGTCAACTCT GTCGACAGTG AACCTTATCC AGACGGCTAC 
AAATACATCC CCGAAAACTG TGTCACTTCC CCCATGAACA TCGACAGGAA CATCACACAC 
TTGCAGTACT GCGTTTGTAA AGAAGACTGT TCAACAAGTA TCTGCATGTG TGGACAACTC 
AGTCTGCGCT GCTGGTACGA CAAGGCTGGT CGTCTTCTAC CTGAATTCTG CCGTGAGGAG 
CCTCCTCTCA TCTTTGAATG TAACCATGCA TGCTCCTGTT GGAGGACCTG TAAGAACCGT 
GTAGTGCAAA ACGGACTCAG GACGAGGCTT CAGCTCTTCA GGACCAATAA GAAGGGCTGG 
GGTGTTCGTG CAGTACAAGA CATACCACGG GGGACCTTTA TATGCGAGTA CGTAGGTGAG 
ATCATCTCGG AAGCCGAGGC AGAGATGAGG CAGAATGACG CTTACCTGTT CAGTCTGGAT 
GATAAGCCTC AAGATCTGTA CTGCATCGAT GCCCGTTTCT ATGGAAACAT CAGCCGCTTC 
CTTAATCACA TGTGCGAGCC CAACTTGTTT GCCTGTCGAG TGTTCACGAC ACACCAGGAC 
CTCCGTTTCC CACACATTGC CTTTTTTGCC AGTGAAAACA TCACAGCTGG AGAGGAGCTT 
GGATTTAACT ATGGCGACCA CTTCTGGGAA GTCAAAAGCA AACTGTTCAA CTGCGAATGT 
GGCTCTTCTA AGTGCAAGTA CTCGTCAGCA GCCATGGCAT CGCTGCAGGC GGATAGCACA 
CCAGAGGACC GGCAGCAGCC CAGCGCGTCA CCTGACACCA GCTCTTCCAA CAGTCCTTCC 
AGTCCTTCTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_019272862.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128407.1 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X4, mRNA.

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CloneID	OLa106345
Clone ID Related Accession (Same CDS sequence)	XM_019272862.1 , XM_019272862.2
Accession Version	XM_019272862.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017609266.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG TTGGACCCGA GCCCGCTGGT CTAGCCAAGG GCAGTGTGGA TAAGGGTGCC 
TCCACAAAAA AAGAGGAACT CAATGCAGCA AGCAACGGAG AGGAGAAGCC AGATGGGGAC 
GAAGAGGTAG CAGCCAGACT GGCCTCCACT GCAGCAGAGG CAATGCTAAA CGGCACTGAA 
TGTGACGACG CGAGGCACAA GAGCCCGACA CACGGCTCTG CAACGGGCAG CAAGAAGACT 
TTACTGTTGG TTAACGAAAA CGGCACGTCA GACACAGAGC CACCGCACGG TTCTGTCACT 
GGAAGCAATG GGTTCATTCT CACTAAGCAG CAGCAGGAAG ATGGCTCTGC AGCGACGCCG 
GGTTTGGGAG TTTCCCCTCA TAGGACTAAC TGGTTGCCTT CGGGCTCACC AGCAGGGGGG 
AGCACAGCCA AACCCCTCCC TGCCTTGGCG TCTGGGTGTG CACAGAGTTT AGGTGCACTA 
AGGACTGACC CCAGCATGGG GACTACATCG GGACAAGGGA CACCAGACAC TAAAAATGGC 
ACAGCACCAG CTCCAACACC GGCCCCAGCA CCCGTCACGA TACACAGAGC GCGCAAGACC 
ATGTCCAGAC CTGCTGTCAG TGCGGCACAA AAGCTTCTTA ACAGGGAATT AAGAGAAGCA 
AAGAGTGCCA AAATGGAGAA GACGGAGGCT CATGTTGCAC CCGACGCCCA GAAGTCCTCG 
TCGCAGTCCT CCGCTCAGAA CCACCTACCT CAGAGTCCAC CGGATACGCC AGCTTCAGCA 
CAAACGGCTT CATCAGCTCC ACCAACGCCT CCAGCGCCTC CACCACCACC CCTGGCTCCC 
GCACCACACT CTGCTCCTCC AGCTCCGGCC AAACTCCACC TAGGCTCTTA CTCAGGAGGG 
TTGTCATCCC GTAAGAAGAA AAGGAGGATG GGAACGTACA GTCTGGTTCC CAAGAAGAAA 
GCCAAAGTGC TCAGGCAGAG AACTATGATG GAAATGTATA AGGATTTACG ACACTCCAAG 
AGTCCACAGA CTAAAGAGGT ATCAAACATA AATGGGGAGA AGGTTGAAAA TGCATCAGAG 
GAGGAGGAGT CCGAGGAGGT GGAGTCGGAG GAAGAGGAGC GACAACAGCC AGCGGAAGAA 
CCTGTCAGCG TTGCCCAGGA AGCAAGATCT GAACCCATCT CTCAGGAGGC GGGAGACGAG 
CAGGAGTCTG AGGAGTCTGG AGAAGAAGAG GGAGAGGAGG AGGGCACAGA GTCTGACTTG 
AGCACAGAGT CCAGTCTGAA GAAAAAGTTG AAAAAGAAAA CAAAAGCAGA CAGCGCGTGG 
CTCAGGCCGT CCAGGAAACG CAAGAGGAGG CTAAAGGCCA AAGTCTTCTG CTCAGAACCA 
GAGGCGGTGG ATCAACCTCA GGCCTCAGCC CACACCCCGG CAGGCGATAG GAAGGAGTAC 
ACGCAGATTC CCTCGCCTGA GTCGGTCAAC CTCAACAAGC CCCAAGAGCC GACGGTTTCC 
TCACAGGTCA AAGACTCTCT GGGGTCGGCC ATTGAGGAGG CTCAGGAGCT TCCGCTCTGC 
AGCTGCCGTA TGGAGACGCC CAAGAGTCGA GAGATTCTCA TCCTGGCGGA CAGGAAGTGC 
ATGGCCACAG AGAGCGTGGA TGGACAGCTG ACCCGCTGCC AGGGTGCCAT ACTGAAACAT 
GAAATGATGC GTCCCTCCAA CTCCGTTCAG CTGCTGGTTC TGTGCGAGGA CCACCGTAAC 
GGCATGGTGA AGCACCAGTG CTGCCCCGGG TGTGGCTTCT TCTGCAGGGC TGGGACCTTC 
ATGGAGTGCC AACCGGACGT CAGCATCTCT CACCGTTTCC ATCGTGCTTG TGCCTCAATG 
CTGAAAGGTC AAAGCTTCTG TCCCCACTGC GGAGAGGAGG CCAGCAAGGC CAAGGAGGTC 
ACCATCGCCA AGGCCGACAC CACCTCCACT GTGCCTCCTG GGCTGACACA CGGACCTGCC 
ACCACCGGGG TCCCCGAGGG CCGAGCAGAC ACCACCACTG GCAGTTCTTC TCGACTGGCC 
GTGGGGGCTG AAGTCAGCGG GAAGGCCGAC AGTTCGCTGC CCATCCGTTC TGCACATGGA 
CTCGACACCC CTGCTGTTCC CGGGTCATCT AGGACCGCAC TGCTGCAGGC CGGGACAGGA 
ACAATGGACT CACCCACAAT CCCTCAAGGA ACGCAGAGAG AGACTCTAGA GAGCATCCTG 
GTAGCTCTCG ACACAGAGAA ACCCAAGAAG CTACGTTTTC ACCCCAAACA ACTTTACCTC 
TCAGCCAAAC AGGGAGAACT CAAGAAGGTC TTGCTTATGT TGGTGGACGG TATCGACCCA 
AATTTTAAGA TGGAATCTCA GAACAAACGC ACACCTCTCC ATGCCGCAGC TGAGGGAGGA 
CACAAAGACA TCTGTCACAT GCTCGTACAA GCTGGTGCGA ACTTGGACAT GTGTGATGAA 
GATCAGCGGA CGCCACTCAT GGAGGCGTGT GAGAACAACC ACATGGAGAC TGTCCTGTAC 
CTGCTGAGAG CTGGAGCAAG CGCCACGCAC AAGGATGTTG AAGGGTTCAC GTGTCTCCAC 
CTAGCGGCAA AGTCTGGTCA TTACAACATT GTCGAGCACC TTCTGTCCAC AGGATTGATT 
AACATAAACT GTCAGGACGA TGGCGGTTGG ACAGCCATGA TCTGGGCGAC AGAGTACAAG 
CATGTGGACC AGGTGAAGCT ACTTCTGTCC AAAGGAGCTG ACATCAGCAT CAGGGACAAG 
GAAGAGAATA TCTGCCTTCA CTGGGCAGCA TTCTCCGGCA GTGTGGACAT CGCCGAGCTG 
CTCCTGAACG CCCGCTGTGA TCTGCAGGTC GTCAACATCC ATGGAGACTC TCCTCTGCAC 
ATCGCTGCCC GGGAGAATCG TCTGGACTGT GTCACACTTT TTCTGTCCCG AGGGGCAGAT 
GTGTTTCTGA AGAACCGCGA GGGAGAAACT CCTCCAGACT GCTGCAGCCA CAACTCAAAG 
GCCTGGGCAG CCCTGCAGGC CCACAGGAGG GAGAAGGATG CCAAGAACAG CAGGCTCAAT 
CGAACAGAGC AAAAAGTCCT TCACAGTGAC ATCGCTCTGG GGCAGGAGCG TGTTCCCATT 
CCCTGTGTCA ACTCTGTCGA CAGTGAACCT TATCCAGACG GCTACAAATA CATCCCCGAA 
AACTGTGTCA CTTCCCCCAT GAACATCGAC AGGAACATCA CACACTTGCA GTACTGCGTT 
TGTAAAGAAG ACTGTTCAAC AAGTATCTGC ATGTGTGGAC AACTCAGTCT GCGCTGCTGG 
TACGACAAGG CTGGTCGTCT TCTACCTGAA TTCTGCCGTG AGGAGCCTCC TCTCATCTTT 
GAATGTAACC ATGCATGCTC CTGTTGGAGG ACCTGTAAGA ACCGTGTAGT GCAAAACGGA 
CTCAGGACGA GGCTTCAGCT CTTCAGGACC AATAAGAAGG GCTGGGGTGT TCGTGCAGTA 
CAAGACATAC CACGGGGGAC CTTTATATGC GAGTACGTAG GTGAGATCAT CTCGGAAGCC 
GAGGCAGAGA TGAGGCAGAA TGACGCTTAC CTGTTCAGTC TGGATGATAA GCCTCAAGAT 
CTGTACTGCA TCGATGCCCG TTTCTATGGA AACATCAGCC GCTTCCTTAA TCACATGTGC 
GAGCCCAACT TGTTTGCCTG TCGAGTGTTC ACGACACACC AGGACCTCCG TTTCCCACAC 
ATTGCCTTTT TTGCCAGTGA AAACATCACA GCTGGAGAGG AGCTTGGATT TAACTATGGC 
GACCACTTCT GGGAAGTCAA AAGCAAACTG TTCAACTGCG AATGTGGCTC TTCTAAGTGC 
AAGTACTCGT CAGCAGCCAT GGCATCGCTG CAGGCGGATA GCACACCAGA GGACCGGCAG 
CAGCCCAGCG CGTCACCTGA CACCAGCTCT TCCAACAGTC CTTCCAGTCC TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128407.1
CDS	19..3975
Translation	MASVGPEPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQEAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKVFCSEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_019272862.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272862.1

ORF Insert Sequence:

ATGGCTTCGG TTGGACCCGA GCCCGCTGGT CTAGCCAAGG GCAGTGTGGA TAAGGGTGCC 
TCCACAAAAA AAGAGGAACT CAATGCAGCA AGCAACGGAG AGGAGAAGCC AGATGGGGAC 
GAAGAGGTAG CAGCCAGACT GGCCTCCACT GCAGCAGAGG CAATGCTAAA CGGCACTGAA 
TGTGACGACG CGAGGCACAA GAGCCCGACA CACGGCTCTG CAACGGGCAG CAAGAAGACT 
TTACTGTTGG TTAACGAAAA CGGCACGTCA GACACAGAGC CACCGCACGG TTCTGTCACT 
GGAAGCAATG GGTTCATTCT CACTAAGCAG CAGCAGGAAG ATGGCTCTGC AGCGACGCCG 
GGTTTGGGAG TTTCCCCTCA TAGGACTAAC TGGTTGCCTT CGGGCTCACC AGCAGGGGGG 
AGCACAGCCA AACCCCTCCC TGCCTTGGCG TCTGGGTGTG CACAGAGTTT AGGTGCACTA 
AGGACTGACC CCAGCATGGG GACTACATCG GGACAAGGGA CACCAGACAC TAAAAATGGC 
ACAGCACCAG CTCCAACACC GGCCCCAGCA CCCGTCACGA TACACAGAGC GCGCAAGACC 
ATGTCCAGAC CTGCTGTCAG TGCGGCACAA AAGCTTCTTA ACAGGGAATT AAGAGAAGCA 
AAGAGTGCCA AAATGGAGAA GACGGAGGCT CATGTTGCAC CCGACGCCCA GAAGTCCTCG 
TCGCAGTCCT CCGCTCAGAA CCACCTACCT CAGAGTCCAC CGGATACGCC AGCTTCAGCA 
CAAACGGCTT CATCAGCTCC ACCAACGCCT CCAGCGCCTC CACCACCACC CCTGGCTCCC 
GCACCACACT CTGCTCCTCC AGCTCCGGCC AAACTCCACC TAGGCTCTTA CTCAGGAGGG 
TTGTCATCCC GTAAGAAGAA AAGGAGGATG GGAACGTACA GTCTGGTTCC CAAGAAGAAA 
GCCAAAGTGC TCAGGCAGAG AACTATGATG GAAATGTATA AGGATTTACG ACACTCCAAG 
AGTCCACAGA CTAAAGAGGT ATCAAACATA AATGGGGAGA AGGTTGAAAA TGCATCAGAG 
GAGGAGGAGT CCGAGGAGGT GGAGTCGGAG GAAGAGGAGC GACAACAGCC AGCGGAAGAA 
CCTGTCAGCG TTGCCCAGGA AGCAAGATCT GAACCCATCT CTCAGGAGGC GGGAGACGAG 
CAGGAGTCTG AGGAGTCTGG AGAAGAAGAG GGAGAGGAGG AGGGCACAGA GTCTGACTTG 
AGCACAGAGT CCAGTCTGAA GAAAAAGTTG AAAAAGAAAA CAAAAGCAGA CAGCGCGTGG 
CTCAGGCCGT CCAGGAAACG CAAGAGGAGG CTAAAGGCCA AAGTCTTCTG CTCAGAACCA 
GAGGCGGTGG ATCAACCTCA GGCCTCAGCC CACACCCCGG CAGGCGATAG GAAGGAGTAC 
ACGCAGATTC CCTCGCCTGA GTCGGTCAAC CTCAACAAGC CCCAAGAGCC GACGGTTTCC 
TCACAGGTCA AAGACTCTCT GGGGTCGGCC ATTGAGGAGG CTCAGGAGCT TCCGCTCTGC 
AGCTGCCGTA TGGAGACGCC CAAGAGTCGA GAGATTCTCA TCCTGGCGGA CAGGAAGTGC 
ATGGCCACAG AGAGCGTGGA TGGACAGCTG ACCCGCTGCC AGGGTGCCAT ACTGAAACAT 
GAAATGATGC GTCCCTCCAA CTCCGTTCAG CTGCTGGTTC TGTGCGAGGA CCACCGTAAC 
GGCATGGTGA AGCACCAGTG CTGCCCCGGG TGTGGCTTCT TCTGCAGGGC TGGGACCTTC 
ATGGAGTGCC AACCGGACGT CAGCATCTCT CACCGTTTCC ATCGTGCTTG TGCCTCAATG 
CTGAAAGGTC AAAGCTTCTG TCCCCACTGC GGAGAGGAGG CCAGCAAGGC CAAGGAGGTC 
ACCATCGCCA AGGCCGACAC CACCTCCACT GTGCCTCCTG GGCTGACACA CGGACCTGCC 
ACCACCGGGG TCCCCGAGGG CCGAGCAGAC ACCACCACTG GCAGTTCTTC TCGACTGGCC 
GTGGGGGCTG AAGTCAGCGG GAAGGCCGAC AGTTCGCTGC CCATCCGTTC TGCACATGGA 
CTCGACACCC CTGCTGTTCC CGGGTCATCT AGGACCGCAC TGCTGCAGGC CGGGACAGGA 
ACAATGGACT CACCCACAAT CCCTCAAGGA ACGCAGAGAG AGACTCTAGA GAGCATCCTG 
GTAGCTCTCG ACACAGAGAA ACCCAAGAAG CTACGTTTTC ACCCCAAACA ACTTTACCTC 
TCAGCCAAAC AGGGAGAACT CAAGAAGGTC TTGCTTATGT TGGTGGACGG TATCGACCCA 
AATTTTAAGA TGGAATCTCA GAACAAACGC ACACCTCTCC ATGCCGCAGC TGAGGGAGGA 
CACAAAGACA TCTGTCACAT GCTCGTACAA GCTGGTGCGA ACTTGGACAT GTGTGATGAA 
GATCAGCGGA CGCCACTCAT GGAGGCGTGT GAGAACAACC ACATGGAGAC TGTCCTGTAC 
CTGCTGAGAG CTGGAGCAAG CGCCACGCAC AAGGATGTTG AAGGGTTCAC GTGTCTCCAC 
CTAGCGGCAA AGTCTGGTCA TTACAACATT GTCGAGCACC TTCTGTCCAC AGGATTGATT 
AACATAAACT GTCAGGACGA TGGCGGTTGG ACAGCCATGA TCTGGGCGAC AGAGTACAAG 
CATGTGGACC AGGTGAAGCT ACTTCTGTCC AAAGGAGCTG ACATCAGCAT CAGGGACAAG 
GAAGAGAATA TCTGCCTTCA CTGGGCAGCA TTCTCCGGCA GTGTGGACAT CGCCGAGCTG 
CTCCTGAACG CCCGCTGTGA TCTGCAGGTC GTCAACATCC ATGGAGACTC TCCTCTGCAC 
ATCGCTGCCC GGGAGAATCG TCTGGACTGT GTCACACTTT TTCTGTCCCG AGGGGCAGAT 
GTGTTTCTGA AGAACCGCGA GGGAGAAACT CCTCCAGACT GCTGCAGCCA CAACTCAAAG 
GCCTGGGCAG CCCTGCAGGC CCACAGGAGG GAGAAGGATG CCAAGAACAG CAGGCTCAAT 
CGAACAGAGC AAAAAGTCCT TCACAGTGAC ATCGCTCTGG GGCAGGAGCG TGTTCCCATT 
CCCTGTGTCA ACTCTGTCGA CAGTGAACCT TATCCAGACG GCTACAAATA CATCCCCGAA 
AACTGTGTCA CTTCCCCCAT GAACATCGAC AGGAACATCA CACACTTGCA GTACTGCGTT 
TGTAAAGAAG ACTGTTCAAC AAGTATCTGC ATGTGTGGAC AACTCAGTCT GCGCTGCTGG 
TACGACAAGG CTGGTCGTCT TCTACCTGAA TTCTGCCGTG AGGAGCCTCC TCTCATCTTT 
GAATGTAACC ATGCATGCTC CTGTTGGAGG ACCTGTAAGA ACCGTGTAGT GCAAAACGGA 
CTCAGGACGA GGCTTCAGCT CTTCAGGACC AATAAGAAGG GCTGGGGTGT TCGTGCAGTA 
CAAGACATAC CACGGGGGAC CTTTATATGC GAGTACGTAG GTGAGATCAT CTCGGAAGCC 
GAGGCAGAGA TGAGGCAGAA TGACGCTTAC CTGTTCAGTC TGGATGATAA GCCTCAAGAT 
CTGTACTGCA TCGATGCCCG TTTCTATGGA AACATCAGCC GCTTCCTTAA TCACATGTGC 
GAGCCCAACT TGTTTGCCTG TCGAGTGTTC ACGACACACC AGGACCTCCG TTTCCCACAC 
ATTGCCTTTT TTGCCAGTGA AAACATCACA GCTGGAGAGG AGCTTGGATT TAACTATGGC 
GACCACTTCT GGGAAGTCAA AAGCAAACTG TTCAACTGCG AATGTGGCTC TTCTAAGTGC 
AAGTACTCGT CAGCAGCCAT GGCATCGCTG CAGGCGGATA GCACACCAGA GGACCGGCAG 
CAGCCCAGCG CGTCACCTGA CACCAGCTCT TCCAACAGTC CTTCCAGTCC TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_019272862.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128407.1 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OLa106345
Clone ID Related Accession (Same CDS sequence)	XM_019272862.1 , XM_019272862.2
Accession Version	XM_019272862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019272862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTCGG TTGGACCCGA GCCCGCTGGT CTAGCCAAGG GCAGTGTGGA TAAGGGTGCC 
TCCACAAAAA AAGAGGAACT CAATGCAGCA AGCAACGGAG AGGAGAAGCC AGATGGGGAC 
GAAGAGGTAG CAGCCAGACT GGCCTCCACT GCAGCAGAGG CAATGCTAAA CGGCACTGAA 
TGTGACGACG CGAGGCACAA GAGCCCGACA CACGGCTCTG CAACGGGCAG CAAGAAGACT 
TTACTGTTGG TTAACGAAAA CGGCACGTCA GACACAGAGC CACCGCACGG TTCTGTCACT 
GGAAGCAATG GGTTCATTCT CACTAAGCAG CAGCAGGAAG ATGGCTCTGC AGCGACGCCG 
GGTTTGGGAG TTTCCCCTCA TAGGACTAAC TGGTTGCCTT CGGGCTCACC AGCAGGGGGG 
AGCACAGCCA AACCCCTCCC TGCCTTGGCG TCTGGGTGTG CACAGAGTTT AGGTGCACTA 
AGGACTGACC CCAGCATGGG GACTACATCG GGACAAGGGA CACCAGACAC TAAAAATGGC 
ACAGCACCAG CTCCAACACC GGCCCCAGCA CCCGTCACGA TACACAGAGC GCGCAAGACC 
ATGTCCAGAC CTGCTGTCAG TGCGGCACAA AAGCTTCTTA ACAGGGAATT AAGAGAAGCA 
AAGAGTGCCA AAATGGAGAA GACGGAGGCT CATGTTGCAC CCGACGCCCA GAAGTCCTCG 
TCGCAGTCCT CCGCTCAGAA CCACCTACCT CAGAGTCCAC CGGATACGCC AGCTTCAGCA 
CAAACGGCTT CATCAGCTCC ACCAACGCCT CCAGCGCCTC CACCACCACC CCTGGCTCCC 
GCACCACACT CTGCTCCTCC AGCTCCGGCC AAACTCCACC TAGGCTCTTA CTCAGGAGGG 
TTGTCATCCC GTAAGAAGAA AAGGAGGATG GGAACGTACA GTCTGGTTCC CAAGAAGAAA 
GCCAAAGTGC TCAGGCAGAG AACTATGATG GAAATGTATA AGGATTTACG ACACTCCAAG 
AGTCCACAGA CTAAAGAGGT ATCAAACATA AATGGGGAGA AGGTTGAAAA TGCATCAGAG 
GAGGAGGAGT CCGAGGAGGT GGAGTCGGAG GAAGAGGAGC GACAACAGCC AGCGGAAGAA 
CCTGTCAGCG TTGCCCAGGA AGCAAGATCT GAACCCATCT CTCAGGAGGC GGGAGACGAG 
CAGGAGTCTG AGGAGTCTGG AGAAGAAGAG GGAGAGGAGG AGGGCACAGA GTCTGACTTG 
AGCACAGAGT CCAGTCTGAA GAAAAAGTTG AAAAAGAAAA CAAAAGCAGA CAGCGCGTGG 
CTCAGGCCGT CCAGGAAACG CAAGAGGAGG CTAAAGGCCA AAGTCTTCTG CTCAGAACCA 
GAGGCGGTGG ATCAACCTCA GGCCTCAGCC CACACCCCGG CAGGCGATAG GAAGGAGTAC 
ACGCAGATTC CCTCGCCTGA GTCGGTCAAC CTCAACAAGC CCCAAGAGCC GACGGTTTCC 
TCACAGGTCA AAGACTCTCT GGGGTCGGCC ATTGAGGAGG CTCAGGAGCT TCCGCTCTGC 
AGCTGCCGTA TGGAGACGCC CAAGAGTCGA GAGATTCTCA TCCTGGCGGA CAGGAAGTGC 
ATGGCCACAG AGAGCGTGGA TGGACAGCTG ACCCGCTGCC AGGGTGCCAT ACTGAAACAT 
GAAATGATGC GTCCCTCCAA CTCCGTTCAG CTGCTGGTTC TGTGCGAGGA CCACCGTAAC 
GGCATGGTGA AGCACCAGTG CTGCCCCGGG TGTGGCTTCT TCTGCAGGGC TGGGACCTTC 
ATGGAGTGCC AACCGGACGT CAGCATCTCT CACCGTTTCC ATCGTGCTTG TGCCTCAATG 
CTGAAAGGTC AAAGCTTCTG TCCCCACTGC GGAGAGGAGG CCAGCAAGGC CAAGGAGGTC 
ACCATCGCCA AGGCCGACAC CACCTCCACT GTGCCTCCTG GGCTGACACA CGGACCTGCC 
ACCACCGGGG TCCCCGAGGG CCGAGCAGAC ACCACCACTG GCAGTTCTTC TCGACTGGCC 
GTGGGGGCTG AAGTCAGCGG GAAGGCCGAC AGTTCGCTGC CCATCCGTTC TGCACATGGA 
CTCGACACCC CTGCTGTTCC CGGGTCATCT AGGACCGCAC TGCTGCAGGC CGGGACAGGA 
ACAATGGACT CACCCACAAT CCCTCAAGGA ACGCAGAGAG AGACTCTAGA GAGCATCCTG 
GTAGCTCTCG ACACAGAGAA ACCCAAGAAG CTACGTTTTC ACCCCAAACA ACTTTACCTC 
TCAGCCAAAC AGGGAGAACT CAAGAAGGTC TTGCTTATGT TGGTGGACGG TATCGACCCA 
AATTTTAAGA TGGAATCTCA GAACAAACGC ACACCTCTCC ATGCCGCAGC TGAGGGAGGA 
CACAAAGACA TCTGTCACAT GCTCGTACAA GCTGGTGCGA ACTTGGACAT GTGTGATGAA 
GATCAGCGGA CGCCACTCAT GGAGGCGTGT GAGAACAACC ACATGGAGAC TGTCCTGTAC 
CTGCTGAGAG CTGGAGCAAG CGCCACGCAC AAGGATGTTG AAGGGTTCAC GTGTCTCCAC 
CTAGCGGCAA AGTCTGGTCA TTACAACATT GTCGAGCACC TTCTGTCCAC AGGATTGATT 
AACATAAACT GTCAGGACGA TGGCGGTTGG ACAGCCATGA TCTGGGCGAC AGAGTACAAG 
CATGTGGACC AGGTGAAGCT ACTTCTGTCC AAAGGAGCTG ACATCAGCAT CAGGGACAAG 
GAAGAGAATA TCTGCCTTCA CTGGGCAGCA TTCTCCGGCA GTGTGGACAT CGCCGAGCTG 
CTCCTGAACG CCCGCTGTGA TCTGCAGGTC GTCAACATCC ATGGAGACTC TCCTCTGCAC 
ATCGCTGCCC GGGAGAATCG TCTGGACTGT GTCACACTTT TTCTGTCCCG AGGGGCAGAT 
GTGTTTCTGA AGAACCGCGA GGGAGAAACT CCTCCAGACT GCTGCAGCCA CAACTCAAAG 
GCCTGGGCAG CCCTGCAGGC CCACAGGAGG GAGAAGGATG CCAAGAACAG CAGGCTCAAT 
CGAACAGAGC AAAAAGTCCT TCACAGTGAC ATCGCTCTGG GGCAGGAGCG TGTTCCCATT 
CCCTGTGTCA ACTCTGTCGA CAGTGAACCT TATCCAGACG GCTACAAATA CATCCCCGAA 
AACTGTGTCA CTTCCCCCAT GAACATCGAC AGGAACATCA CACACTTGCA GTACTGCGTT 
TGTAAAGAAG ACTGTTCAAC AAGTATCTGC ATGTGTGGAC AACTCAGTCT GCGCTGCTGG 
TACGACAAGG CTGGTCGTCT TCTACCTGAA TTCTGCCGTG AGGAGCCTCC TCTCATCTTT 
GAATGTAACC ATGCATGCTC CTGTTGGAGG ACCTGTAAGA ACCGTGTAGT GCAAAACGGA 
CTCAGGACGA GGCTTCAGCT CTTCAGGACC AATAAGAAGG GCTGGGGTGT TCGTGCAGTA 
CAAGACATAC CACGGGGGAC CTTTATATGC GAGTACGTAG GTGAGATCAT CTCGGAAGCC 
GAGGCAGAGA TGAGGCAGAA TGACGCTTAC CTGTTCAGTC TGGATGATAA GCCTCAAGAT 
CTGTACTGCA TCGATGCCCG TTTCTATGGA AACATCAGCC GCTTCCTTAA TCACATGTGC 
GAGCCCAACT TGTTTGCCTG TCGAGTGTTC ACGACACACC AGGACCTCCG TTTCCCACAC 
ATTGCCTTTT TTGCCAGTGA AAACATCACA GCTGGAGAGG AGCTTGGATT TAACTATGGC 
GACCACTTCT GGGAAGTCAA AAGCAAACTG TTCAACTGCG AATGTGGCTC TTCTAAGTGC 
AAGTACTCGT CAGCAGCCAT GGCATCGCTG CAGGCGGATA GCACACCAGA GGACCGGCAG 
CAGCCCAGCG CGTCACCTGA CACCAGCTCT TCCAACAGTC CTTCCAGTCC TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128407.1
CDS	42..3998
Translation	MASVGPEPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQEAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKVFCSEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_019272862.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272862.2

ORF Insert Sequence:

ATGGCTTCGG TTGGACCCGA GCCCGCTGGT CTAGCCAAGG GCAGTGTGGA TAAGGGTGCC 
TCCACAAAAA AAGAGGAACT CAATGCAGCA AGCAACGGAG AGGAGAAGCC AGATGGGGAC 
GAAGAGGTAG CAGCCAGACT GGCCTCCACT GCAGCAGAGG CAATGCTAAA CGGCACTGAA 
TGTGACGACG CGAGGCACAA GAGCCCGACA CACGGCTCTG CAACGGGCAG CAAGAAGACT 
TTACTGTTGG TTAACGAAAA CGGCACGTCA GACACAGAGC CACCGCACGG TTCTGTCACT 
GGAAGCAATG GGTTCATTCT CACTAAGCAG CAGCAGGAAG ATGGCTCTGC AGCGACGCCG 
GGTTTGGGAG TTTCCCCTCA TAGGACTAAC TGGTTGCCTT CGGGCTCACC AGCAGGGGGG 
AGCACAGCCA AACCCCTCCC TGCCTTGGCG TCTGGGTGTG CACAGAGTTT AGGTGCACTA 
AGGACTGACC CCAGCATGGG GACTACATCG GGACAAGGGA CACCAGACAC TAAAAATGGC 
ACAGCACCAG CTCCAACACC GGCCCCAGCA CCCGTCACGA TACACAGAGC GCGCAAGACC 
ATGTCCAGAC CTGCTGTCAG TGCGGCACAA AAGCTTCTTA ACAGGGAATT AAGAGAAGCA 
AAGAGTGCCA AAATGGAGAA GACGGAGGCT CATGTTGCAC CCGACGCCCA GAAGTCCTCG 
TCGCAGTCCT CCGCTCAGAA CCACCTACCT CAGAGTCCAC CGGATACGCC AGCTTCAGCA 
CAAACGGCTT CATCAGCTCC ACCAACGCCT CCAGCGCCTC CACCACCACC CCTGGCTCCC 
GCACCACACT CTGCTCCTCC AGCTCCGGCC AAACTCCACC TAGGCTCTTA CTCAGGAGGG 
TTGTCATCCC GTAAGAAGAA AAGGAGGATG GGAACGTACA GTCTGGTTCC CAAGAAGAAA 
GCCAAAGTGC TCAGGCAGAG AACTATGATG GAAATGTATA AGGATTTACG ACACTCCAAG 
AGTCCACAGA CTAAAGAGGT ATCAAACATA AATGGGGAGA AGGTTGAAAA TGCATCAGAG 
GAGGAGGAGT CCGAGGAGGT GGAGTCGGAG GAAGAGGAGC GACAACAGCC AGCGGAAGAA 
CCTGTCAGCG TTGCCCAGGA AGCAAGATCT GAACCCATCT CTCAGGAGGC GGGAGACGAG 
CAGGAGTCTG AGGAGTCTGG AGAAGAAGAG GGAGAGGAGG AGGGCACAGA GTCTGACTTG 
AGCACAGAGT CCAGTCTGAA GAAAAAGTTG AAAAAGAAAA CAAAAGCAGA CAGCGCGTGG 
CTCAGGCCGT CCAGGAAACG CAAGAGGAGG CTAAAGGCCA AAGTCTTCTG CTCAGAACCA 
GAGGCGGTGG ATCAACCTCA GGCCTCAGCC CACACCCCGG CAGGCGATAG GAAGGAGTAC 
ACGCAGATTC CCTCGCCTGA GTCGGTCAAC CTCAACAAGC CCCAAGAGCC GACGGTTTCC 
TCACAGGTCA AAGACTCTCT GGGGTCGGCC ATTGAGGAGG CTCAGGAGCT TCCGCTCTGC 
AGCTGCCGTA TGGAGACGCC CAAGAGTCGA GAGATTCTCA TCCTGGCGGA CAGGAAGTGC 
ATGGCCACAG AGAGCGTGGA TGGACAGCTG ACCCGCTGCC AGGGTGCCAT ACTGAAACAT 
GAAATGATGC GTCCCTCCAA CTCCGTTCAG CTGCTGGTTC TGTGCGAGGA CCACCGTAAC 
GGCATGGTGA AGCACCAGTG CTGCCCCGGG TGTGGCTTCT TCTGCAGGGC TGGGACCTTC 
ATGGAGTGCC AACCGGACGT CAGCATCTCT CACCGTTTCC ATCGTGCTTG TGCCTCAATG 
CTGAAAGGTC AAAGCTTCTG TCCCCACTGC GGAGAGGAGG CCAGCAAGGC CAAGGAGGTC 
ACCATCGCCA AGGCCGACAC CACCTCCACT GTGCCTCCTG GGCTGACACA CGGACCTGCC 
ACCACCGGGG TCCCCGAGGG CCGAGCAGAC ACCACCACTG GCAGTTCTTC TCGACTGGCC 
GTGGGGGCTG AAGTCAGCGG GAAGGCCGAC AGTTCGCTGC CCATCCGTTC TGCACATGGA 
CTCGACACCC CTGCTGTTCC CGGGTCATCT AGGACCGCAC TGCTGCAGGC CGGGACAGGA 
ACAATGGACT CACCCACAAT CCCTCAAGGA ACGCAGAGAG AGACTCTAGA GAGCATCCTG 
GTAGCTCTCG ACACAGAGAA ACCCAAGAAG CTACGTTTTC ACCCCAAACA ACTTTACCTC 
TCAGCCAAAC AGGGAGAACT CAAGAAGGTC TTGCTTATGT TGGTGGACGG TATCGACCCA 
AATTTTAAGA TGGAATCTCA GAACAAACGC ACACCTCTCC ATGCCGCAGC TGAGGGAGGA 
CACAAAGACA TCTGTCACAT GCTCGTACAA GCTGGTGCGA ACTTGGACAT GTGTGATGAA 
GATCAGCGGA CGCCACTCAT GGAGGCGTGT GAGAACAACC ACATGGAGAC TGTCCTGTAC 
CTGCTGAGAG CTGGAGCAAG CGCCACGCAC AAGGATGTTG AAGGGTTCAC GTGTCTCCAC 
CTAGCGGCAA AGTCTGGTCA TTACAACATT GTCGAGCACC TTCTGTCCAC AGGATTGATT 
AACATAAACT GTCAGGACGA TGGCGGTTGG ACAGCCATGA TCTGGGCGAC AGAGTACAAG 
CATGTGGACC AGGTGAAGCT ACTTCTGTCC AAAGGAGCTG ACATCAGCAT CAGGGACAAG 
GAAGAGAATA TCTGCCTTCA CTGGGCAGCA TTCTCCGGCA GTGTGGACAT CGCCGAGCTG 
CTCCTGAACG CCCGCTGTGA TCTGCAGGTC GTCAACATCC ATGGAGACTC TCCTCTGCAC 
ATCGCTGCCC GGGAGAATCG TCTGGACTGT GTCACACTTT TTCTGTCCCG AGGGGCAGAT 
GTGTTTCTGA AGAACCGCGA GGGAGAAACT CCTCCAGACT GCTGCAGCCA CAACTCAAAG 
GCCTGGGCAG CCCTGCAGGC CCACAGGAGG GAGAAGGATG CCAAGAACAG CAGGCTCAAT 
CGAACAGAGC AAAAAGTCCT TCACAGTGAC ATCGCTCTGG GGCAGGAGCG TGTTCCCATT 
CCCTGTGTCA ACTCTGTCGA CAGTGAACCT TATCCAGACG GCTACAAATA CATCCCCGAA 
AACTGTGTCA CTTCCCCCAT GAACATCGAC AGGAACATCA CACACTTGCA GTACTGCGTT 
TGTAAAGAAG ACTGTTCAAC AAGTATCTGC ATGTGTGGAC AACTCAGTCT GCGCTGCTGG 
TACGACAAGG CTGGTCGTCT TCTACCTGAA TTCTGCCGTG AGGAGCCTCC TCTCATCTTT 
GAATGTAACC ATGCATGCTC CTGTTGGAGG ACCTGTAAGA ACCGTGTAGT GCAAAACGGA 
CTCAGGACGA GGCTTCAGCT CTTCAGGACC AATAAGAAGG GCTGGGGTGT TCGTGCAGTA 
CAAGACATAC CACGGGGGAC CTTTATATGC GAGTACGTAG GTGAGATCAT CTCGGAAGCC 
GAGGCAGAGA TGAGGCAGAA TGACGCTTAC CTGTTCAGTC TGGATGATAA GCCTCAAGAT 
CTGTACTGCA TCGATGCCCG TTTCTATGGA AACATCAGCC GCTTCCTTAA TCACATGTGC 
GAGCCCAACT TGTTTGCCTG TCGAGTGTTC ACGACACACC AGGACCTCCG TTTCCCACAC 
ATTGCCTTTT TTGCCAGTGA AAACATCACA GCTGGAGAGG AGCTTGGATT TAACTATGGC 
GACCACTTCT GGGAAGTCAA AAGCAAACTG TTCAACTGCG AATGTGGCTC TTCTAAGTGC 
AAGTACTCGT CAGCAGCCAT GGCATCGCTG CAGGCGGATA GCACACCAGA GGACCGGCAG 
CAGCCCAGCG CGTCACCTGA CACCAGCTCT TCCAACAGTC CTTCCAGTCC TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_019272859.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128404.2 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X1, mRNA.

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CloneID	OLa219523
Clone ID Related Accession (Same CDS sequence)	XM_019272859.2
Accession Version	XM_019272859.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019272859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCA TGAGAAGAAA GCAGCCCGCT GGTCTAGCCA AGGGCAGTGT GGATAAGGGT 
GCCTCCACAA AAAAAGAGGA ACTCAATGCA GCAAGCAACG GAGAGGAGAA GCCAGATGGG 
GACGAAGAGG TAGCAGCCAG ACTGGCCTCC ACTGCAGCAG AGGCAATGCT AAACGGCACT 
GAATGTGACG ACGCGAGGCA CAAGAGCCCG ACACACGGCT CTGCAACGGG CAGCAAGAAG 
ACTTTACTGT TGGTTAACGA AAACGGCACG TCAGACACAG AGCCACCGCA CGGTTCTGTC 
ACTGGAAGCA ATGGGTTCAT TCTCACTAAG CAGCAGCAGG AAGATGGCTC TGCAGCGACG 
CCGGGTTTGG GAGTTTCCCC TCATAGGACT AACTGGTTGC CTTCGGGCTC ACCAGCAGGG 
GGGAGCACAG CCAAACCCCT CCCTGCCTTG GCGTCTGGGT GTGCACAGAG TTTAGGTGCA 
CTAAGGACTG ACCCCAGCAT GGGGACTACA TCGGGACAAG GGACACCAGA CACTAAAAAT 
GGCACAGCAC CAGCTCCAAC ACCGGCCCCA GCACCCGTCA CGATACACAG AGCGCGCAAG 
ACCATGTCCA GACCTGCTGT CAGTGCGGCA CAAAAGCTTC TTAACAGGGA ATTAAGAGAA 
GCAAAGAGTG CCAAAATGGA GAAGACGGAG GCTCATGTTG CACCCGACGC CCAGAAGTCC 
TCGTCGCAGT CCTCCGCTCA GAACCACCTA CCTCAGAGTC CACCGGATAC GCCAGCTTCA 
GCACAAACGG CTTCATCAGC TCCACCAACG CCTCCAGCGC CTCCACCACC ACCCCTGGCT 
CCCGCACCAC ACTCTGCTCC TCCAGCTCCG GCCAAACTCC ACCTAGGCTC TTACTCAGGA 
GGGTTGTCAT CCCGTAAGAA GAAAAGGAGG ATGGGAACGT ACAGTCTGGT TCCCAAGAAG 
AAAGCCAAAG TGCTCAGGCA GAGAACTATG ATGGAAATGT ATAAGGATTT ACGACACTCC 
AAGAGTCCAC AGACTAAAGA GGTATCAAAC ATAAATGGGG AGAAGGTTGA AAATGCATCA 
GAGGAGGAGG AGTCCGAGGA GGTGGAGTCG GAGGAAGAGG AGCGACAACA GCCAGCGGAA 
GAACCTGTCA GCGTTGCCCA GGAAGCAAGA TCTGAACCCA TCTCTCAGGA GGCGGGAGAC 
GAGCAGGAGT CTGAGGAGTC TGGAGAAGAA GAGGGAGAGG AGGAGGGCAC AGAGTCTGAC 
TTGAGCACAG AGTCCAGTCT GAAGAAAAAG TTGAAAAAGA AAACAAAAGC AGACAGCGCG 
TGGCTCAGGC CGTCCAGGAA ACGCAAGAGG AGGCTAAAGG CCAAAGTCTT CTGCTCAGAA 
CCAGAGGCGG TGGATCAACC TCAGGCCTCA GCCCACACCC CGGCAGGCGA TAGGAAGGAG 
TACACGCAGA TTCCCTCGCC TGAGTCGGTC AACCTCAACA AGCCCCAAGA GCCGACGGTT 
TCCTCACAGG TCAAAGACTC TCTGGGGTCG GCCATTGAGG AGGCTCAGGA GCTTCCGCTC 
TGCAGCTGCC GTATGGAGAC GCCCAAGAGT CGAGAGATTC TCATCCTGGC GGACAGGAAG 
TGCATGGCCA CAGAGAGCGT GGATGGACAG CTGACCCGCT GCCAGGGTGC CATACTGAAA 
CATGAAATGA TGCGTCCCTC CAACTCCGTT CAGCTGCTGG TTCTGTGCGA GGACCACCGT 
AACGGCATGG TGAAGCACCA GTGCTGCCCC GGGTGTGGCT TCTTCTGCAG GGCTGGGACC 
TTCATGGAGT GCCAACCGGA CGTCAGCATC TCTCACCGTT TCCATCGTGC TTGTGCCTCA 
ATGCTGAAAG GTCAAAGCTT CTGTCCCCAC TGCGGAGAGG AGGCCAGCAA GGCCAAGGAG 
GTCACCATCG CCAAGGCCGA CACCACCTCC ACTGTGCCTC CTGGGCTGAC ACACGGACCT 
GCCACCACCG GGGTCCCCGA GGGCCGAGCA GACACCACCA CTGGCAGTTC TTCTCGACTG 
GCCGTGGGGG CTGAAGTCAG CGGGAAGGCC GACAGTTCGC TGCCCATCCG TTCTGCACAT 
GGACTCGACA CCCCTGCTGT TCCCGGGTCA TCTAGGACCG CACTGCTGCA GGCCGGGACA 
GGAACAATGG ACTCACCCAC AATCCCTCAA GGAACGCAGA GAGAGACTCT AGAGAGCATC 
CTGGTAGCTC TCGACACAGA GAAACCCAAG AAGCTACGTT TTCACCCCAA ACAACTTTAC 
CTCTCAGCCA AACAGGGAGA ACTCAAGAAG GTCTTGCTTA TGTTGGTGGA CGGTATCGAC 
CCAAATTTTA AGATGGAATC TCAGAACAAA CGCACACCTC TCCATGCCGC AGCTGAGGGA 
GGACACAAAG ACATCTGTCA CATGCTCGTA CAAGCTGGTG CGAACTTGGA CATGTGTGAT 
GAAGATCAGC GGACGCCACT CATGGAGGCG TGTGAGAACA ACCACATGGA GACTGTCCTG 
TACCTGCTGA GAGCTGGAGC AAGCGCCACG CACAAGGATG TTGAAGGGTT CACGTGTCTC 
CACCTAGCGG CAAAGTCTGG TCATTACAAC ATTGTCGAGC ACCTTCTGTC CACAGGATTG 
ATTAACATAA ACTGTCAGGA CGATGGCGGT TGGACAGCCA TGATCTGGGC GACAGAGTAC 
AAGCATGTGG ACCAGGTGAA GCTACTTCTG TCCAAAGGAG CTGACATCAG CATCAGGGAC 
AAGGAAGAGA ATATCTGCCT TCACTGGGCA GCATTCTCCG GCAGTGTGGA CATCGCCGAG 
CTGCTCCTGA ACGCCCGCTG TGATCTGCAG GTCGTCAACA TCCATGGAGA CTCTCCTCTG 
CACATCGCTG CCCGGGAGAA TCGTCTGGAC TGTGTCACAC TTTTTCTGTC CCGAGGGGCA 
GATGTGTTTC TGAAGAACCG CGAGGGAGAA ACTCCTCCAG ACTGCTGCAG CCACAACTCA 
AAGGCCTGGG CAGCCCTGCA GGCCCACAGG AGGGAGAAGG ATGCCAAGAA CAGCAGGCTC 
AATCGAACAG AGCAAAAAGT CCTTCACAGT GACATCGCTC TGGGGCAGGA GCGTGTTCCC 
ATTCCCTGTG TCAACTCTGT CGACAGTGAA CCTTATCCAG ACGGCTACAA ATACATCCCC 
GAAAACTGTG TCACTTCCCC CATGAACATC GACAGGAACA TCACACACTT GCAGTACTGC 
GTTTGTAAAG AAGACTGTTC AACAAGTATC TGCATGTGTG GACAACTCAG TCTGCGCTGC 
TGGTACGACA AGGCTGGTCG TCTTCTACCT GAATTCTGCC GTGAGGAGCC TCCTCTCATC 
TTTGAATGTA ACCATGCATG CTCCTGTTGG AGGACCTGTA AGAACCGTGT AGTGCAAAAC 
GGACTCAGGA CGAGGCTTCA GCTCTTCAGG ACCAATAAGA AGGGCTGGGG TGTTCGTGCA 
GTACAAGACA TACCACGGGG GACCTTTATA TGCGAGTACG TAGGTGAGAT CATCTCGGAA 
GCCGAGGCAG AGATGAGGCA GAATGACGCT TACCTGTTCA GTCTGGATGA TAAGCCTCAA 
GATCTGTACT GCATCGATGC CCGTTTCTAT GGAAACATCA GCCGCTTCCT TAATCACATG 
TGCGAGCCCA ACTTGTTTGC CTGTCGAGTG TTCACGACAC ACCAGGACCT CCGTTTCCCA 
CACATTGCCT TTTTTGCCAG TGAAAACATC ACAGCTGGAG AGGAGCTTGG ATTTAACTAT 
GGCGACCACT TCTGGGAAGT CAAAAGCAAA CTGTTCAACT GCGAATGTGG CTCTTCTAAG 
TGCAAGTACT CGTCAGCAGC CATGGCATCG CTGCAGGCGG ATAGCACACC AGAGGACCGG 
CAGCAGCCCA GCGCGTCACC TGACACCAGC TCTTCCAACA GTCCTTCCAG TCCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128404.2
CDS	88..4047
Translation	MEAMRRKQPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQEAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKVFCSEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_019272859.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272859.2

ORF Insert Sequence:

ATGGAGGCCA TGAGAAGAAA GCAGCCCGCT GGTCTAGCCA AGGGCAGTGT GGATAAGGGT 
GCCTCCACAA AAAAAGAGGA ACTCAATGCA GCAAGCAACG GAGAGGAGAA GCCAGATGGG 
GACGAAGAGG TAGCAGCCAG ACTGGCCTCC ACTGCAGCAG AGGCAATGCT AAACGGCACT 
GAATGTGACG ACGCGAGGCA CAAGAGCCCG ACACACGGCT CTGCAACGGG CAGCAAGAAG 
ACTTTACTGT TGGTTAACGA AAACGGCACG TCAGACACAG AGCCACCGCA CGGTTCTGTC 
ACTGGAAGCA ATGGGTTCAT TCTCACTAAG CAGCAGCAGG AAGATGGCTC TGCAGCGACG 
CCGGGTTTGG GAGTTTCCCC TCATAGGACT AACTGGTTGC CTTCGGGCTC ACCAGCAGGG 
GGGAGCACAG CCAAACCCCT CCCTGCCTTG GCGTCTGGGT GTGCACAGAG TTTAGGTGCA 
CTAAGGACTG ACCCCAGCAT GGGGACTACA TCGGGACAAG GGACACCAGA CACTAAAAAT 
GGCACAGCAC CAGCTCCAAC ACCGGCCCCA GCACCCGTCA CGATACACAG AGCGCGCAAG 
ACCATGTCCA GACCTGCTGT CAGTGCGGCA CAAAAGCTTC TTAACAGGGA ATTAAGAGAA 
GCAAAGAGTG CCAAAATGGA GAAGACGGAG GCTCATGTTG CACCCGACGC CCAGAAGTCC 
TCGTCGCAGT CCTCCGCTCA GAACCACCTA CCTCAGAGTC CACCGGATAC GCCAGCTTCA 
GCACAAACGG CTTCATCAGC TCCACCAACG CCTCCAGCGC CTCCACCACC ACCCCTGGCT 
CCCGCACCAC ACTCTGCTCC TCCAGCTCCG GCCAAACTCC ACCTAGGCTC TTACTCAGGA 
GGGTTGTCAT CCCGTAAGAA GAAAAGGAGG ATGGGAACGT ACAGTCTGGT TCCCAAGAAG 
AAAGCCAAAG TGCTCAGGCA GAGAACTATG ATGGAAATGT ATAAGGATTT ACGACACTCC 
AAGAGTCCAC AGACTAAAGA GGTATCAAAC ATAAATGGGG AGAAGGTTGA AAATGCATCA 
GAGGAGGAGG AGTCCGAGGA GGTGGAGTCG GAGGAAGAGG AGCGACAACA GCCAGCGGAA 
GAACCTGTCA GCGTTGCCCA GGAAGCAAGA TCTGAACCCA TCTCTCAGGA GGCGGGAGAC 
GAGCAGGAGT CTGAGGAGTC TGGAGAAGAA GAGGGAGAGG AGGAGGGCAC AGAGTCTGAC 
TTGAGCACAG AGTCCAGTCT GAAGAAAAAG TTGAAAAAGA AAACAAAAGC AGACAGCGCG 
TGGCTCAGGC CGTCCAGGAA ACGCAAGAGG AGGCTAAAGG CCAAAGTCTT CTGCTCAGAA 
CCAGAGGCGG TGGATCAACC TCAGGCCTCA GCCCACACCC CGGCAGGCGA TAGGAAGGAG 
TACACGCAGA TTCCCTCGCC TGAGTCGGTC AACCTCAACA AGCCCCAAGA GCCGACGGTT 
TCCTCACAGG TCAAAGACTC TCTGGGGTCG GCCATTGAGG AGGCTCAGGA GCTTCCGCTC 
TGCAGCTGCC GTATGGAGAC GCCCAAGAGT CGAGAGATTC TCATCCTGGC GGACAGGAAG 
TGCATGGCCA CAGAGAGCGT GGATGGACAG CTGACCCGCT GCCAGGGTGC CATACTGAAA 
CATGAAATGA TGCGTCCCTC CAACTCCGTT CAGCTGCTGG TTCTGTGCGA GGACCACCGT 
AACGGCATGG TGAAGCACCA GTGCTGCCCC GGGTGTGGCT TCTTCTGCAG GGCTGGGACC 
TTCATGGAGT GCCAACCGGA CGTCAGCATC TCTCACCGTT TCCATCGTGC TTGTGCCTCA 
ATGCTGAAAG GTCAAAGCTT CTGTCCCCAC TGCGGAGAGG AGGCCAGCAA GGCCAAGGAG 
GTCACCATCG CCAAGGCCGA CACCACCTCC ACTGTGCCTC CTGGGCTGAC ACACGGACCT 
GCCACCACCG GGGTCCCCGA GGGCCGAGCA GACACCACCA CTGGCAGTTC TTCTCGACTG 
GCCGTGGGGG CTGAAGTCAG CGGGAAGGCC GACAGTTCGC TGCCCATCCG TTCTGCACAT 
GGACTCGACA CCCCTGCTGT TCCCGGGTCA TCTAGGACCG CACTGCTGCA GGCCGGGACA 
GGAACAATGG ACTCACCCAC AATCCCTCAA GGAACGCAGA GAGAGACTCT AGAGAGCATC 
CTGGTAGCTC TCGACACAGA GAAACCCAAG AAGCTACGTT TTCACCCCAA ACAACTTTAC 
CTCTCAGCCA AACAGGGAGA ACTCAAGAAG GTCTTGCTTA TGTTGGTGGA CGGTATCGAC 
CCAAATTTTA AGATGGAATC TCAGAACAAA CGCACACCTC TCCATGCCGC AGCTGAGGGA 
GGACACAAAG ACATCTGTCA CATGCTCGTA CAAGCTGGTG CGAACTTGGA CATGTGTGAT 
GAAGATCAGC GGACGCCACT CATGGAGGCG TGTGAGAACA ACCACATGGA GACTGTCCTG 
TACCTGCTGA GAGCTGGAGC AAGCGCCACG CACAAGGATG TTGAAGGGTT CACGTGTCTC 
CACCTAGCGG CAAAGTCTGG TCATTACAAC ATTGTCGAGC ACCTTCTGTC CACAGGATTG 
ATTAACATAA ACTGTCAGGA CGATGGCGGT TGGACAGCCA TGATCTGGGC GACAGAGTAC 
AAGCATGTGG ACCAGGTGAA GCTACTTCTG TCCAAAGGAG CTGACATCAG CATCAGGGAC 
AAGGAAGAGA ATATCTGCCT TCACTGGGCA GCATTCTCCG GCAGTGTGGA CATCGCCGAG 
CTGCTCCTGA ACGCCCGCTG TGATCTGCAG GTCGTCAACA TCCATGGAGA CTCTCCTCTG 
CACATCGCTG CCCGGGAGAA TCGTCTGGAC TGTGTCACAC TTTTTCTGTC CCGAGGGGCA 
GATGTGTTTC TGAAGAACCG CGAGGGAGAA ACTCCTCCAG ACTGCTGCAG CCACAACTCA 
AAGGCCTGGG CAGCCCTGCA GGCCCACAGG AGGGAGAAGG ATGCCAAGAA CAGCAGGCTC 
AATCGAACAG AGCAAAAAGT CCTTCACAGT GACATCGCTC TGGGGCAGGA GCGTGTTCCC 
ATTCCCTGTG TCAACTCTGT CGACAGTGAA CCTTATCCAG ACGGCTACAA ATACATCCCC 
GAAAACTGTG TCACTTCCCC CATGAACATC GACAGGAACA TCACACACTT GCAGTACTGC 
GTTTGTAAAG AAGACTGTTC AACAAGTATC TGCATGTGTG GACAACTCAG TCTGCGCTGC 
TGGTACGACA AGGCTGGTCG TCTTCTACCT GAATTCTGCC GTGAGGAGCC TCCTCTCATC 
TTTGAATGTA ACCATGCATG CTCCTGTTGG AGGACCTGTA AGAACCGTGT AGTGCAAAAC 
GGACTCAGGA CGAGGCTTCA GCTCTTCAGG ACCAATAAGA AGGGCTGGGG TGTTCGTGCA 
GTACAAGACA TACCACGGGG GACCTTTATA TGCGAGTACG TAGGTGAGAT CATCTCGGAA 
GCCGAGGCAG AGATGAGGCA GAATGACGCT TACCTGTTCA GTCTGGATGA TAAGCCTCAA 
GATCTGTACT GCATCGATGC CCGTTTCTAT GGAAACATCA GCCGCTTCCT TAATCACATG 
TGCGAGCCCA ACTTGTTTGC CTGTCGAGTG TTCACGACAC ACCAGGACCT CCGTTTCCCA 
CACATTGCCT TTTTTGCCAG TGAAAACATC ACAGCTGGAG AGGAGCTTGG ATTTAACTAT 
GGCGACCACT TCTGGGAAGT CAAAAGCAAA CTGTTCAACT GCGAATGTGG CTCTTCTAAG 
TGCAAGTACT CGTCAGCAGC CATGGCATCG CTGCAGGCGG ATAGCACACC AGAGGACCGG 
CAGCAGCCCA GCGCGTCACC TGACACCAGC TCTTCCAACA GTCCTTCCAG TCCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_019272860.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128405.2 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X2, mRNA.

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CloneID	OLa219524
Clone ID Related Accession (Same CDS sequence)	XM_019272860.2
Accession Version	XM_019272860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019272860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCA TGAGAAGAAA GCAGCCCGCT GGTCTAGCCA AGGGCAGTGT GGATAAGGGT 
GCCTCCACAA AAAAAGAGGA ACTCAATGCA GCAAGCAACG GAGAGGAGAA GCCAGATGGG 
GACGAAGAGG TAGCAGCCAG ACTGGCCTCC ACTGCAGCAG AGGCAATGCT AAACGGCACT 
GAATGTGACG ACGCGAGGCA CAAGAGCCCG ACACACGGCT CTGCAACGGG CAGCAAGAAG 
ACTTTACTGT TGGTTAACGA AAACGGCACG TCAGACACAG AGCCACCGCA CGGTTCTGTC 
ACTGGAAGCA ATGGGTTCAT TCTCACTAAG CAGCAGCAGG AAGATGGCTC TGCAGCGACG 
CCGGGTTTGG GAGTTTCCCC TCATAGGACT AACTGGTTGC CTTCGGGCTC ACCAGCAGGG 
GGGAGCACAG CCAAACCCCT CCCTGCCTTG GCGTCTGGGT GTGCACAGAG TTTAGGTGCA 
CTAAGGACTG ACCCCAGCAT GGGGACTACA TCGGGACAAG GGACACCAGA CACTAAAAAT 
GGCACAGCAC CAGCTCCAAC ACCGGCCCCA GCACCCGTCA CGATACACAG AGCGCGCAAG 
ACCATGTCCA GACCTGCTGT CAGTGCGGCA CAAAAGCTTC TTAACAGGGA ATTAAGAGAA 
GCAAAGAGTG CCAAAATGGA GAAGACGGAG GCTCATGTTG CACCCGACGC CCAGAAGTCC 
TCGTCGCAGT CCTCCGCTCA GAACCACCTA CCTCAGAGTC CACCGGATAC GCCAGCTTCA 
GCACAAACGG CTTCATCAGC TCCACCAACG CCTCCAGCGC CTCCACCACC ACCCCTGGCT 
CCCGCACCAC ACTCTGCTCC TCCAGCTCCG GCCAAACTCC ACCTAGGCTC TTACTCAGGA 
GGGTTGTCAT CCCGTAAGAA GAAAAGGAGG ATGGGAACGT ACAGTCTGGT TCCCAAGAAG 
AAAGCCAAAG TGCTCAGGCA GAGAACTATG ATGGAAATGT ATAAGGATTT ACGACACTCC 
AAGAGTCCAC AGACTAAAGA GGTATCAAAC ATAAATGGGG AGAAGGTTGA AAATGCATCA 
GAGGAGGAGG AGTCCGAGGA GGTGGAGTCG GAGGAAGAGG AGCGACAACA GCCAGCGGAA 
GAACCTGTCA GCGTTGCCCA GGAAGCAAGA TCTGAACCCA TCTCTCAGGC GGGAGACGAG 
CAGGAGTCTG AGGAGTCTGG AGAAGAAGAG GGAGAGGAGG AGGGCACAGA GTCTGACTTG 
AGCACAGAGT CCAGTCTGAA GAAAAAGTTG AAAAAGAAAA CAAAAGCAGA CAGCGCGTGG 
CTCAGGCCGT CCAGGAAACG CAAGAGGAGG CTAAAGGCCA AAGTCTTCTG CTCAGAACCA 
GAGGCGGTGG ATCAACCTCA GGCCTCAGCC CACACCCCGG CAGGCGATAG GAAGGAGTAC 
ACGCAGATTC CCTCGCCTGA GTCGGTCAAC CTCAACAAGC CCCAAGAGCC GACGGTTTCC 
TCACAGGTCA AAGACTCTCT GGGGTCGGCC ATTGAGGAGG CTCAGGAGCT TCCGCTCTGC 
AGCTGCCGTA TGGAGACGCC CAAGAGTCGA GAGATTCTCA TCCTGGCGGA CAGGAAGTGC 
ATGGCCACAG AGAGCGTGGA TGGACAGCTG ACCCGCTGCC AGGGTGCCAT ACTGAAACAT 
GAAATGATGC GTCCCTCCAA CTCCGTTCAG CTGCTGGTTC TGTGCGAGGA CCACCGTAAC 
GGCATGGTGA AGCACCAGTG CTGCCCCGGG TGTGGCTTCT TCTGCAGGGC TGGGACCTTC 
ATGGAGTGCC AACCGGACGT CAGCATCTCT CACCGTTTCC ATCGTGCTTG TGCCTCAATG 
CTGAAAGGTC AAAGCTTCTG TCCCCACTGC GGAGAGGAGG CCAGCAAGGC CAAGGAGGTC 
ACCATCGCCA AGGCCGACAC CACCTCCACT GTGCCTCCTG GGCTGACACA CGGACCTGCC 
ACCACCGGGG TCCCCGAGGG CCGAGCAGAC ACCACCACTG GCAGTTCTTC TCGACTGGCC 
GTGGGGGCTG AAGTCAGCGG GAAGGCCGAC AGTTCGCTGC CCATCCGTTC TGCACATGGA 
CTCGACACCC CTGCTGTTCC CGGGTCATCT AGGACCGCAC TGCTGCAGGC CGGGACAGGA 
ACAATGGACT CACCCACAAT CCCTCAAGGA ACGCAGAGAG AGACTCTAGA GAGCATCCTG 
GTAGCTCTCG ACACAGAGAA ACCCAAGAAG CTACGTTTTC ACCCCAAACA ACTTTACCTC 
TCAGCCAAAC AGGGAGAACT CAAGAAGGTC TTGCTTATGT TGGTGGACGG TATCGACCCA 
AATTTTAAGA TGGAATCTCA GAACAAACGC ACACCTCTCC ATGCCGCAGC TGAGGGAGGA 
CACAAAGACA TCTGTCACAT GCTCGTACAA GCTGGTGCGA ACTTGGACAT GTGTGATGAA 
GATCAGCGGA CGCCACTCAT GGAGGCGTGT GAGAACAACC ACATGGAGAC TGTCCTGTAC 
CTGCTGAGAG CTGGAGCAAG CGCCACGCAC AAGGATGTTG AAGGGTTCAC GTGTCTCCAC 
CTAGCGGCAA AGTCTGGTCA TTACAACATT GTCGAGCACC TTCTGTCCAC AGGATTGATT 
AACATAAACT GTCAGGACGA TGGCGGTTGG ACAGCCATGA TCTGGGCGAC AGAGTACAAG 
CATGTGGACC AGGTGAAGCT ACTTCTGTCC AAAGGAGCTG ACATCAGCAT CAGGGACAAG 
GAAGAGAATA TCTGCCTTCA CTGGGCAGCA TTCTCCGGCA GTGTGGACAT CGCCGAGCTG 
CTCCTGAACG CCCGCTGTGA TCTGCAGGTC GTCAACATCC ATGGAGACTC TCCTCTGCAC 
ATCGCTGCCC GGGAGAATCG TCTGGACTGT GTCACACTTT TTCTGTCCCG AGGGGCAGAT 
GTGTTTCTGA AGAACCGCGA GGGAGAAACT CCTCCAGACT GCTGCAGCCA CAACTCAAAG 
GCCTGGGCAG CCCTGCAGGC CCACAGGAGG GAGAAGGATG CCAAGAACAG CAGGCTCAAT 
CGAACAGAGC AAAAAGTCCT TCACAGTGAC ATCGCTCTGG GGCAGGAGCG TGTTCCCATT 
CCCTGTGTCA ACTCTGTCGA CAGTGAACCT TATCCAGACG GCTACAAATA CATCCCCGAA 
AACTGTGTCA CTTCCCCCAT GAACATCGAC AGGAACATCA CACACTTGCA GTACTGCGTT 
TGTAAAGAAG ACTGTTCAAC AAGTATCTGC ATGTGTGGAC AACTCAGTCT GCGCTGCTGG 
TACGACAAGG CTGGTCGTCT TCTACCTGAA TTCTGCCGTG AGGAGCCTCC TCTCATCTTT 
GAATGTAACC ATGCATGCTC CTGTTGGAGG ACCTGTAAGA ACCGTGTAGT GCAAAACGGA 
CTCAGGACGA GGCTTCAGCT CTTCAGGACC AATAAGAAGG GCTGGGGTGT TCGTGCAGTA 
CAAGACATAC CACGGGGGAC CTTTATATGC GAGTACGTAG GTGAGATCAT CTCGGAAGCC 
GAGGCAGAGA TGAGGCAGAA TGACGCTTAC CTGTTCAGTC TGGATGATAA GCCTCAAGAT 
CTGTACTGCA TCGATGCCCG TTTCTATGGA AACATCAGCC GCTTCCTTAA TCACATGTGC 
GAGCCCAACT TGTTTGCCTG TCGAGTGTTC ACGACACACC AGGACCTCCG TTTCCCACAC 
ATTGCCTTTT TTGCCAGTGA AAACATCACA GCTGGAGAGG AGCTTGGATT TAACTATGGC 
GACCACTTCT GGGAAGTCAA AAGCAAACTG TTCAACTGCG AATGTGGCTC TTCTAAGTGC 
AAGTACTCGT CAGCAGCCAT GGCATCGCTG CAGGCGGATA GCACACCAGA GGACCGGCAG 
CAGCCCAGCG CGTCACCTGA CACCAGCTCT TCCAACAGTC CTTCCAGTCC TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128405.2
CDS	88..4044
Translation	MEAMRRKQPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKVFCSEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_019272860.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272860.2

ORF Insert Sequence:

ATGGAGGCCA TGAGAAGAAA GCAGCCCGCT GGTCTAGCCA AGGGCAGTGT GGATAAGGGT 
GCCTCCACAA AAAAAGAGGA ACTCAATGCA GCAAGCAACG GAGAGGAGAA GCCAGATGGG 
GACGAAGAGG TAGCAGCCAG ACTGGCCTCC ACTGCAGCAG AGGCAATGCT AAACGGCACT 
GAATGTGACG ACGCGAGGCA CAAGAGCCCG ACACACGGCT CTGCAACGGG CAGCAAGAAG 
ACTTTACTGT TGGTTAACGA AAACGGCACG TCAGACACAG AGCCACCGCA CGGTTCTGTC 
ACTGGAAGCA ATGGGTTCAT TCTCACTAAG CAGCAGCAGG AAGATGGCTC TGCAGCGACG 
CCGGGTTTGG GAGTTTCCCC TCATAGGACT AACTGGTTGC CTTCGGGCTC ACCAGCAGGG 
GGGAGCACAG CCAAACCCCT CCCTGCCTTG GCGTCTGGGT GTGCACAGAG TTTAGGTGCA 
CTAAGGACTG ACCCCAGCAT GGGGACTACA TCGGGACAAG GGACACCAGA CACTAAAAAT 
GGCACAGCAC CAGCTCCAAC ACCGGCCCCA GCACCCGTCA CGATACACAG AGCGCGCAAG 
ACCATGTCCA GACCTGCTGT CAGTGCGGCA CAAAAGCTTC TTAACAGGGA ATTAAGAGAA 
GCAAAGAGTG CCAAAATGGA GAAGACGGAG GCTCATGTTG CACCCGACGC CCAGAAGTCC 
TCGTCGCAGT CCTCCGCTCA GAACCACCTA CCTCAGAGTC CACCGGATAC GCCAGCTTCA 
GCACAAACGG CTTCATCAGC TCCACCAACG CCTCCAGCGC CTCCACCACC ACCCCTGGCT 
CCCGCACCAC ACTCTGCTCC TCCAGCTCCG GCCAAACTCC ACCTAGGCTC TTACTCAGGA 
GGGTTGTCAT CCCGTAAGAA GAAAAGGAGG ATGGGAACGT ACAGTCTGGT TCCCAAGAAG 
AAAGCCAAAG TGCTCAGGCA GAGAACTATG ATGGAAATGT ATAAGGATTT ACGACACTCC 
AAGAGTCCAC AGACTAAAGA GGTATCAAAC ATAAATGGGG AGAAGGTTGA AAATGCATCA 
GAGGAGGAGG AGTCCGAGGA GGTGGAGTCG GAGGAAGAGG AGCGACAACA GCCAGCGGAA 
GAACCTGTCA GCGTTGCCCA GGAAGCAAGA TCTGAACCCA TCTCTCAGGC GGGAGACGAG 
CAGGAGTCTG AGGAGTCTGG AGAAGAAGAG GGAGAGGAGG AGGGCACAGA GTCTGACTTG 
AGCACAGAGT CCAGTCTGAA GAAAAAGTTG AAAAAGAAAA CAAAAGCAGA CAGCGCGTGG 
CTCAGGCCGT CCAGGAAACG CAAGAGGAGG CTAAAGGCCA AAGTCTTCTG CTCAGAACCA 
GAGGCGGTGG ATCAACCTCA GGCCTCAGCC CACACCCCGG CAGGCGATAG GAAGGAGTAC 
ACGCAGATTC CCTCGCCTGA GTCGGTCAAC CTCAACAAGC CCCAAGAGCC GACGGTTTCC 
TCACAGGTCA AAGACTCTCT GGGGTCGGCC ATTGAGGAGG CTCAGGAGCT TCCGCTCTGC 
AGCTGCCGTA TGGAGACGCC CAAGAGTCGA GAGATTCTCA TCCTGGCGGA CAGGAAGTGC 
ATGGCCACAG AGAGCGTGGA TGGACAGCTG ACCCGCTGCC AGGGTGCCAT ACTGAAACAT 
GAAATGATGC GTCCCTCCAA CTCCGTTCAG CTGCTGGTTC TGTGCGAGGA CCACCGTAAC 
GGCATGGTGA AGCACCAGTG CTGCCCCGGG TGTGGCTTCT TCTGCAGGGC TGGGACCTTC 
ATGGAGTGCC AACCGGACGT CAGCATCTCT CACCGTTTCC ATCGTGCTTG TGCCTCAATG 
CTGAAAGGTC AAAGCTTCTG TCCCCACTGC GGAGAGGAGG CCAGCAAGGC CAAGGAGGTC 
ACCATCGCCA AGGCCGACAC CACCTCCACT GTGCCTCCTG GGCTGACACA CGGACCTGCC 
ACCACCGGGG TCCCCGAGGG CCGAGCAGAC ACCACCACTG GCAGTTCTTC TCGACTGGCC 
GTGGGGGCTG AAGTCAGCGG GAAGGCCGAC AGTTCGCTGC CCATCCGTTC TGCACATGGA 
CTCGACACCC CTGCTGTTCC CGGGTCATCT AGGACCGCAC TGCTGCAGGC CGGGACAGGA 
ACAATGGACT CACCCACAAT CCCTCAAGGA ACGCAGAGAG AGACTCTAGA GAGCATCCTG 
GTAGCTCTCG ACACAGAGAA ACCCAAGAAG CTACGTTTTC ACCCCAAACA ACTTTACCTC 
TCAGCCAAAC AGGGAGAACT CAAGAAGGTC TTGCTTATGT TGGTGGACGG TATCGACCCA 
AATTTTAAGA TGGAATCTCA GAACAAACGC ACACCTCTCC ATGCCGCAGC TGAGGGAGGA 
CACAAAGACA TCTGTCACAT GCTCGTACAA GCTGGTGCGA ACTTGGACAT GTGTGATGAA 
GATCAGCGGA CGCCACTCAT GGAGGCGTGT GAGAACAACC ACATGGAGAC TGTCCTGTAC 
CTGCTGAGAG CTGGAGCAAG CGCCACGCAC AAGGATGTTG AAGGGTTCAC GTGTCTCCAC 
CTAGCGGCAA AGTCTGGTCA TTACAACATT GTCGAGCACC TTCTGTCCAC AGGATTGATT 
AACATAAACT GTCAGGACGA TGGCGGTTGG ACAGCCATGA TCTGGGCGAC AGAGTACAAG 
CATGTGGACC AGGTGAAGCT ACTTCTGTCC AAAGGAGCTG ACATCAGCAT CAGGGACAAG 
GAAGAGAATA TCTGCCTTCA CTGGGCAGCA TTCTCCGGCA GTGTGGACAT CGCCGAGCTG 
CTCCTGAACG CCCGCTGTGA TCTGCAGGTC GTCAACATCC ATGGAGACTC TCCTCTGCAC 
ATCGCTGCCC GGGAGAATCG TCTGGACTGT GTCACACTTT TTCTGTCCCG AGGGGCAGAT 
GTGTTTCTGA AGAACCGCGA GGGAGAAACT CCTCCAGACT GCTGCAGCCA CAACTCAAAG 
GCCTGGGCAG CCCTGCAGGC CCACAGGAGG GAGAAGGATG CCAAGAACAG CAGGCTCAAT 
CGAACAGAGC AAAAAGTCCT TCACAGTGAC ATCGCTCTGG GGCAGGAGCG TGTTCCCATT 
CCCTGTGTCA ACTCTGTCGA CAGTGAACCT TATCCAGACG GCTACAAATA CATCCCCGAA 
AACTGTGTCA CTTCCCCCAT GAACATCGAC AGGAACATCA CACACTTGCA GTACTGCGTT 
TGTAAAGAAG ACTGTTCAAC AAGTATCTGC ATGTGTGGAC AACTCAGTCT GCGCTGCTGG 
TACGACAAGG CTGGTCGTCT TCTACCTGAA TTCTGCCGTG AGGAGCCTCC TCTCATCTTT 
GAATGTAACC ATGCATGCTC CTGTTGGAGG ACCTGTAAGA ACCGTGTAGT GCAAAACGGA 
CTCAGGACGA GGCTTCAGCT CTTCAGGACC AATAAGAAGG GCTGGGGTGT TCGTGCAGTA 
CAAGACATAC CACGGGGGAC CTTTATATGC GAGTACGTAG GTGAGATCAT CTCGGAAGCC 
GAGGCAGAGA TGAGGCAGAA TGACGCTTAC CTGTTCAGTC TGGATGATAA GCCTCAAGAT 
CTGTACTGCA TCGATGCCCG TTTCTATGGA AACATCAGCC GCTTCCTTAA TCACATGTGC 
GAGCCCAACT TGTTTGCCTG TCGAGTGTTC ACGACACACC AGGACCTCCG TTTCCCACAC 
ATTGCCTTTT TTGCCAGTGA AAACATCACA GCTGGAGAGG AGCTTGGATT TAACTATGGC 
GACCACTTCT GGGAAGTCAA AAGCAAACTG TTCAACTGCG AATGTGGCTC TTCTAAGTGC 
AAGTACTCGT CAGCAGCCAT GGCATCGCTG CAGGCGGATA GCACACCAGA GGACCGGCAG 
CAGCCCAGCG CGTCACCTGA CACCAGCTCT TCCAACAGTC CTTCCAGTCC TTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_019272861.2 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019128406.2 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X3, mRNA.

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CloneID	OLa219525
Clone ID Related Accession (Same CDS sequence)	XM_019272861.2
Accession Version	XM_019272861.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 5, 2018 this sequence version replaced XM_019272861.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCCA TGAGAAGAAA GCAGCCCGCT GGTCTAGCCA AGGGCAGTGT GGATAAGGGT 
GCCTCCACAA AAAAAGAGGA ACTCAATGCA GCAAGCAACG GAGAGGAGAA GCCAGATGGG 
GACGAAGAGG TAGCAGCCAG ACTGGCCTCC ACTGCAGCAG AGGCAATGCT AAACGGCACT 
GAATGTGACG ACGCGAGGCA CAAGAGCCCG ACACACGGCT CTGCAACGGG CAGCAAGAAG 
ACTTTACTGT TGGTTAACGA AAACGGCACG TCAGACACAG AGCCACCGCA CGGTTCTGTC 
ACTGGAAGCA ATGGGTTCAT TCTCACTAAG CAGCAGCAGG AAGATGGCTC TGCAGCGACG 
CCGGGTTTGG GAGTTTCCCC TCATAGGACT AACTGGTTGC CTTCGGGCTC ACCAGCAGGG 
GGGAGCACAG CCAAACCCCT CCCTGCCTTG GCGTCTGGGT GTGCACAGAG TTTAGGTGCA 
CTAAGGACTG ACCCCAGCAT GGGGACTACA TCGGGACAAG GGACACCAGA CACTAAAAAT 
GGCACAGCAC CAGCTCCAAC ACCGGCCCCA GCACCCGTCA CGATACACAG AGCGCGCAAG 
ACCATGTCCA GACCTGCTGT CAGTGCGGCA CAAAAGCTTC TTAACAGGGA ATTAAGAGAA 
GCAAAGAGTG CCAAAATGGA GAAGACGGAG GCTCATGTTG CACCCGACGC CCAGAAGTCC 
TCGTCGCAGT CCTCCGCTCA GAACCACCTA CCTCAGAGTC CACCGGATAC GCCAGCTTCA 
GCACAAACGG CTTCATCAGC TCCACCAACG CCTCCAGCGC CTCCACCACC ACCCCTGGCT 
CCCGCACCAC ACTCTGCTCC TCCAGCTCCG GCCAAACTCC ACCTAGGCTC TTACTCAGGA 
GGGTTGTCAT CCCGTAAGAA GAAAAGGAGG ATGGGAACGT ACAGTCTGGT TCCCAAGAAG 
AAAGCCAAAG TGCTCAGGCA GAGAACTATG ATGGAAATGT ATAAGGATTT ACGACACTCC 
AAGAGTCCAC AGACTAAAGA GGTATCAAAC ATAAATGGGG AGAAGGTTGA AAATGCATCA 
GAGGAGGAGG AGTCCGAGGA GGTGGAGTCG GAGGAAGAGG AGCGACAACA GCCAGCGGAA 
GAACCTGTCA GCGTTGCCCA GGAAGCAAGA TCTGAACCCA TCTCTCAGGA GGCGGGAGAC 
GAGCAGGAGT CTGAGGAGTC TGGAGAAGAA GAGGGAGAGG AGGAGGGCAC AGAGTCTGAC 
TTGAGCACAG AGTCCAGTCT GAAGAAAAAG TTGAAAAAGA AAACAAAAGC AGACAGCGCG 
TGGCTCAGGC CGTCCAGGAA ACGCAAGAGG AGGCTAAAGG CCAAAGAACC AGAGGCGGTG 
GATCAACCTC AGGCCTCAGC CCACACCCCG GCAGGCGATA GGAAGGAGTA CACGCAGATT 
CCCTCGCCTG AGTCGGTCAA CCTCAACAAG CCCCAAGAGC CGACGGTTTC CTCACAGGTC 
AAAGACTCTC TGGGGTCGGC CATTGAGGAG GCTCAGGAGC TTCCGCTCTG CAGCTGCCGT 
ATGGAGACGC CCAAGAGTCG AGAGATTCTC ATCCTGGCGG ACAGGAAGTG CATGGCCACA 
GAGAGCGTGG ATGGACAGCT GACCCGCTGC CAGGGTGCCA TACTGAAACA TGAAATGATG 
CGTCCCTCCA ACTCCGTTCA GCTGCTGGTT CTGTGCGAGG ACCACCGTAA CGGCATGGTG 
AAGCACCAGT GCTGCCCCGG GTGTGGCTTC TTCTGCAGGG CTGGGACCTT CATGGAGTGC 
CAACCGGACG TCAGCATCTC TCACCGTTTC CATCGTGCTT GTGCCTCAAT GCTGAAAGGT 
CAAAGCTTCT GTCCCCACTG CGGAGAGGAG GCCAGCAAGG CCAAGGAGGT CACCATCGCC 
AAGGCCGACA CCACCTCCAC TGTGCCTCCT GGGCTGACAC ACGGACCTGC CACCACCGGG 
GTCCCCGAGG GCCGAGCAGA CACCACCACT GGCAGTTCTT CTCGACTGGC CGTGGGGGCT 
GAAGTCAGCG GGAAGGCCGA CAGTTCGCTG CCCATCCGTT CTGCACATGG ACTCGACACC 
CCTGCTGTTC CCGGGTCATC TAGGACCGCA CTGCTGCAGG CCGGGACAGG AACAATGGAC 
TCACCCACAA TCCCTCAAGG AACGCAGAGA GAGACTCTAG AGAGCATCCT GGTAGCTCTC 
GACACAGAGA AACCCAAGAA GCTACGTTTT CACCCCAAAC AACTTTACCT CTCAGCCAAA 
CAGGGAGAAC TCAAGAAGGT CTTGCTTATG TTGGTGGACG GTATCGACCC AAATTTTAAG 
ATGGAATCTC AGAACAAACG CACACCTCTC CATGCCGCAG CTGAGGGAGG ACACAAAGAC 
ATCTGTCACA TGCTCGTACA AGCTGGTGCG AACTTGGACA TGTGTGATGA AGATCAGCGG 
ACGCCACTCA TGGAGGCGTG TGAGAACAAC CACATGGAGA CTGTCCTGTA CCTGCTGAGA 
GCTGGAGCAA GCGCCACGCA CAAGGATGTT GAAGGGTTCA CGTGTCTCCA CCTAGCGGCA 
AAGTCTGGTC ATTACAACAT TGTCGAGCAC CTTCTGTCCA CAGGATTGAT TAACATAAAC 
TGTCAGGACG ATGGCGGTTG GACAGCCATG ATCTGGGCGA CAGAGTACAA GCATGTGGAC 
CAGGTGAAGC TACTTCTGTC CAAAGGAGCT GACATCAGCA TCAGGGACAA GGAAGAGAAT 
ATCTGCCTTC ACTGGGCAGC ATTCTCCGGC AGTGTGGACA TCGCCGAGCT GCTCCTGAAC 
GCCCGCTGTG ATCTGCAGGT CGTCAACATC CATGGAGACT CTCCTCTGCA CATCGCTGCC 
CGGGAGAATC GTCTGGACTG TGTCACACTT TTTCTGTCCC GAGGGGCAGA TGTGTTTCTG 
AAGAACCGCG AGGGAGAAAC TCCTCCAGAC TGCTGCAGCC ACAACTCAAA GGCCTGGGCA 
GCCCTGCAGG CCCACAGGAG GGAGAAGGAT GCCAAGAACA GCAGGCTCAA TCGAACAGAG 
CAAAAAGTCC TTCACAGTGA CATCGCTCTG GGGCAGGAGC GTGTTCCCAT TCCCTGTGTC 
AACTCTGTCG ACAGTGAACC TTATCCAGAC GGCTACAAAT ACATCCCCGA AAACTGTGTC 
ACTTCCCCCA TGAACATCGA CAGGAACATC ACACACTTGC AGTACTGCGT TTGTAAAGAA 
GACTGTTCAA CAAGTATCTG CATGTGTGGA CAACTCAGTC TGCGCTGCTG GTACGACAAG 
GCTGGTCGTC TTCTACCTGA ATTCTGCCGT GAGGAGCCTC CTCTCATCTT TGAATGTAAC 
CATGCATGCT CCTGTTGGAG GACCTGTAAG AACCGTGTAG TGCAAAACGG ACTCAGGACG 
AGGCTTCAGC TCTTCAGGAC CAATAAGAAG GGCTGGGGTG TTCGTGCAGT ACAAGACATA 
CCACGGGGGA CCTTTATATG CGAGTACGTA GGTGAGATCA TCTCGGAAGC CGAGGCAGAG 
ATGAGGCAGA ATGACGCTTA CCTGTTCAGT CTGGATGATA AGCCTCAAGA TCTGTACTGC 
ATCGATGCCC GTTTCTATGG AAACATCAGC CGCTTCCTTA ATCACATGTG CGAGCCCAAC 
TTGTTTGCCT GTCGAGTGTT CACGACACAC CAGGACCTCC GTTTCCCACA CATTGCCTTT 
TTTGCCAGTG AAAACATCAC AGCTGGAGAG GAGCTTGGAT TTAACTATGG CGACCACTTC 
TGGGAAGTCA AAAGCAAACT GTTCAACTGC GAATGTGGCT CTTCTAAGTG CAAGTACTCG 
TCAGCAGCCA TGGCATCGCT GCAGGCGGAT AGCACACCAG AGGACCGGCA GCAGCCCAGC 
GCGTCACCTG ACACCAGCTC TTCCAACAGT CCTTCCAGTC CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019128406.2
CDS	86..4033
Translation	MEAMRRKQPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQEAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_019272861.2
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019272861.2

ORF Insert Sequence:

ATGGAGGCCA TGAGAAGAAA GCAGCCCGCT GGTCTAGCCA AGGGCAGTGT GGATAAGGGT 
GCCTCCACAA AAAAAGAGGA ACTCAATGCA GCAAGCAACG GAGAGGAGAA GCCAGATGGG 
GACGAAGAGG TAGCAGCCAG ACTGGCCTCC ACTGCAGCAG AGGCAATGCT AAACGGCACT 
GAATGTGACG ACGCGAGGCA CAAGAGCCCG ACACACGGCT CTGCAACGGG CAGCAAGAAG 
ACTTTACTGT TGGTTAACGA AAACGGCACG TCAGACACAG AGCCACCGCA CGGTTCTGTC 
ACTGGAAGCA ATGGGTTCAT TCTCACTAAG CAGCAGCAGG AAGATGGCTC TGCAGCGACG 
CCGGGTTTGG GAGTTTCCCC TCATAGGACT AACTGGTTGC CTTCGGGCTC ACCAGCAGGG 
GGGAGCACAG CCAAACCCCT CCCTGCCTTG GCGTCTGGGT GTGCACAGAG TTTAGGTGCA 
CTAAGGACTG ACCCCAGCAT GGGGACTACA TCGGGACAAG GGACACCAGA CACTAAAAAT 
GGCACAGCAC CAGCTCCAAC ACCGGCCCCA GCACCCGTCA CGATACACAG AGCGCGCAAG 
ACCATGTCCA GACCTGCTGT CAGTGCGGCA CAAAAGCTTC TTAACAGGGA ATTAAGAGAA 
GCAAAGAGTG CCAAAATGGA GAAGACGGAG GCTCATGTTG CACCCGACGC CCAGAAGTCC 
TCGTCGCAGT CCTCCGCTCA GAACCACCTA CCTCAGAGTC CACCGGATAC GCCAGCTTCA 
GCACAAACGG CTTCATCAGC TCCACCAACG CCTCCAGCGC CTCCACCACC ACCCCTGGCT 
CCCGCACCAC ACTCTGCTCC TCCAGCTCCG GCCAAACTCC ACCTAGGCTC TTACTCAGGA 
GGGTTGTCAT CCCGTAAGAA GAAAAGGAGG ATGGGAACGT ACAGTCTGGT TCCCAAGAAG 
AAAGCCAAAG TGCTCAGGCA GAGAACTATG ATGGAAATGT ATAAGGATTT ACGACACTCC 
AAGAGTCCAC AGACTAAAGA GGTATCAAAC ATAAATGGGG AGAAGGTTGA AAATGCATCA 
GAGGAGGAGG AGTCCGAGGA GGTGGAGTCG GAGGAAGAGG AGCGACAACA GCCAGCGGAA 
GAACCTGTCA GCGTTGCCCA GGAAGCAAGA TCTGAACCCA TCTCTCAGGA GGCGGGAGAC 
GAGCAGGAGT CTGAGGAGTC TGGAGAAGAA GAGGGAGAGG AGGAGGGCAC AGAGTCTGAC 
TTGAGCACAG AGTCCAGTCT GAAGAAAAAG TTGAAAAAGA AAACAAAAGC AGACAGCGCG 
TGGCTCAGGC CGTCCAGGAA ACGCAAGAGG AGGCTAAAGG CCAAAGAACC AGAGGCGGTG 
GATCAACCTC AGGCCTCAGC CCACACCCCG GCAGGCGATA GGAAGGAGTA CACGCAGATT 
CCCTCGCCTG AGTCGGTCAA CCTCAACAAG CCCCAAGAGC CGACGGTTTC CTCACAGGTC 
AAAGACTCTC TGGGGTCGGC CATTGAGGAG GCTCAGGAGC TTCCGCTCTG CAGCTGCCGT 
ATGGAGACGC CCAAGAGTCG AGAGATTCTC ATCCTGGCGG ACAGGAAGTG CATGGCCACA 
GAGAGCGTGG ATGGACAGCT GACCCGCTGC CAGGGTGCCA TACTGAAACA TGAAATGATG 
CGTCCCTCCA ACTCCGTTCA GCTGCTGGTT CTGTGCGAGG ACCACCGTAA CGGCATGGTG 
AAGCACCAGT GCTGCCCCGG GTGTGGCTTC TTCTGCAGGG CTGGGACCTT CATGGAGTGC 
CAACCGGACG TCAGCATCTC TCACCGTTTC CATCGTGCTT GTGCCTCAAT GCTGAAAGGT 
CAAAGCTTCT GTCCCCACTG CGGAGAGGAG GCCAGCAAGG CCAAGGAGGT CACCATCGCC 
AAGGCCGACA CCACCTCCAC TGTGCCTCCT GGGCTGACAC ACGGACCTGC CACCACCGGG 
GTCCCCGAGG GCCGAGCAGA CACCACCACT GGCAGTTCTT CTCGACTGGC CGTGGGGGCT 
GAAGTCAGCG GGAAGGCCGA CAGTTCGCTG CCCATCCGTT CTGCACATGG ACTCGACACC 
CCTGCTGTTC CCGGGTCATC TAGGACCGCA CTGCTGCAGG CCGGGACAGG AACAATGGAC 
TCACCCACAA TCCCTCAAGG AACGCAGAGA GAGACTCTAG AGAGCATCCT GGTAGCTCTC 
GACACAGAGA AACCCAAGAA GCTACGTTTT CACCCCAAAC AACTTTACCT CTCAGCCAAA 
CAGGGAGAAC TCAAGAAGGT CTTGCTTATG TTGGTGGACG GTATCGACCC AAATTTTAAG 
ATGGAATCTC AGAACAAACG CACACCTCTC CATGCCGCAG CTGAGGGAGG ACACAAAGAC 
ATCTGTCACA TGCTCGTACA AGCTGGTGCG AACTTGGACA TGTGTGATGA AGATCAGCGG 
ACGCCACTCA TGGAGGCGTG TGAGAACAAC CACATGGAGA CTGTCCTGTA CCTGCTGAGA 
GCTGGAGCAA GCGCCACGCA CAAGGATGTT GAAGGGTTCA CGTGTCTCCA CCTAGCGGCA 
AAGTCTGGTC ATTACAACAT TGTCGAGCAC CTTCTGTCCA CAGGATTGAT TAACATAAAC 
TGTCAGGACG ATGGCGGTTG GACAGCCATG ATCTGGGCGA CAGAGTACAA GCATGTGGAC 
CAGGTGAAGC TACTTCTGTC CAAAGGAGCT GACATCAGCA TCAGGGACAA GGAAGAGAAT 
ATCTGCCTTC ACTGGGCAGC ATTCTCCGGC AGTGTGGACA TCGCCGAGCT GCTCCTGAAC 
GCCCGCTGTG ATCTGCAGGT CGTCAACATC CATGGAGACT CTCCTCTGCA CATCGCTGCC 
CGGGAGAATC GTCTGGACTG TGTCACACTT TTTCTGTCCC GAGGGGCAGA TGTGTTTCTG 
AAGAACCGCG AGGGAGAAAC TCCTCCAGAC TGCTGCAGCC ACAACTCAAA GGCCTGGGCA 
GCCCTGCAGG CCCACAGGAG GGAGAAGGAT GCCAAGAACA GCAGGCTCAA TCGAACAGAG 
CAAAAAGTCC TTCACAGTGA CATCGCTCTG GGGCAGGAGC GTGTTCCCAT TCCCTGTGTC 
AACTCTGTCG ACAGTGAACC TTATCCAGAC GGCTACAAAT ACATCCCCGA AAACTGTGTC 
ACTTCCCCCA TGAACATCGA CAGGAACATC ACACACTTGC AGTACTGCGT TTGTAAAGAA 
GACTGTTCAA CAAGTATCTG CATGTGTGGA CAACTCAGTC TGCGCTGCTG GTACGACAAG 
GCTGGTCGTC TTCTACCTGA ATTCTGCCGT GAGGAGCCTC CTCTCATCTT TGAATGTAAC 
CATGCATGCT CCTGTTGGAG GACCTGTAAG AACCGTGTAG TGCAAAACGG ACTCAGGACG 
AGGCTTCAGC TCTTCAGGAC CAATAAGAAG GGCTGGGGTG TTCGTGCAGT ACAAGACATA 
CCACGGGGGA CCTTTATATG CGAGTACGTA GGTGAGATCA TCTCGGAAGC CGAGGCAGAG 
ATGAGGCAGA ATGACGCTTA CCTGTTCAGT CTGGATGATA AGCCTCAAGA TCTGTACTGC 
ATCGATGCCC GTTTCTATGG AAACATCAGC CGCTTCCTTA ATCACATGTG CGAGCCCAAC 
TTGTTTGCCT GTCGAGTGTT CACGACACAC CAGGACCTCC GTTTCCCACA CATTGCCTTT 
TTTGCCAGTG AAAACATCAC AGCTGGAGAG GAGCTTGGAT TTAACTATGG CGACCACTTC 
TGGGAAGTCA AAAGCAAACT GTTCAACTGC GAATGTGGCT CTTCTAAGTG CAAGTACTCG 
TCAGCAGCCA TGGCATCGCT GCAGGCGGAT AGCACACCAG AGGACCGGCA GCAGCCCAGC 
GCGTCACCTG ACACCAGCTC TTCCAACAGT CCTTCCAGTC CTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_027278613.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027134414.1 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X5, mRNA.

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CloneID	OLa219526
Clone ID Related Accession (Same CDS sequence)	XM_027278613.1
Accession Version	XM_027278613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCTG GTCTAGCCAA GGGCAGTGTG GATAAGGGTG CCTCCACAAA AAAAGAGGAA 
CTCAATGCAG CAAGCAACGG AGAGGAGAAG CCAGATGGGG ACGAAGAGGT AGCAGCCAGA 
CTGGCCTCCA CTGCAGCAGA GGCAATGCTA AACGGCACTG AATGTGACGA CGCGAGGCAC 
AAGAGCCCGA CACACGGCTC TGCAACGGGC AGCAAGAAGA CTTTACTGTT GGTTAACGAA 
AACGGCACGT CAGACACAGA GCCACCGCAC GGTTCTGTCA CTGGAAGCAA TGGGTTCATT 
CTCACTAAGC AGCAGCAGGA AGATGGCTCT GCAGCGACGC CGGGTTTGGG AGTTTCCCCT 
CATAGGACTA ACTGGTTGCC TTCGGGCTCA CCAGCAGGGG GGAGCACAGC CAAACCCCTC 
CCTGCCTTGG CGTCTGGGTG TGCACAGAGT TTAGGTGCAC TAAGGACTGA CCCCAGCATG 
GGGACTACAT CGGGACAAGG GACACCAGAC ACTAAAAATG GCACAGCACC AGCTCCAACA 
CCGGCCCCAG CACCCGTCAC GATACACAGA GCGCGCAAGA CCATGTCCAG ACCTGCTGTC 
AGTGCGGCAC AAAAGCTTCT TAACAGGGAA TTAAGAGAAG CAAAGAGTGC CAAAATGGAG 
AAGACGGAGG CTCATGTTGC ACCCGACGCC CAGAAGTCCT CGTCGCAGTC CTCCGCTCAG 
AACCACCTAC CTCAGAGTCC ACCGGATACG CCAGCTTCAG CACAAACGGC TTCATCAGCT 
CCACCAACGC CTCCAGCGCC TCCACCACCA CCCCTGGCTC CCGCACCACA CTCTGCTCCT 
CCAGCTCCGG CCAAACTCCA CCTAGGCTCT TACTCAGGAG GGTTGTCATC CCGTAAGAAG 
AAAAGGAGGA TGGGAACGTA CAGTCTGGTT CCCAAGAAGA AAGCCAAAGT GCTCAGGCAG 
AGAACTATGA TGGAAATGTA TAAGGATTTA CGACACTCCA AGAGTCCACA GACTAAAGAG 
GTATCAAACA TAAATGGGGA GAAGGTTGAA AATGCATCAG AGGAGGAGGA GTCCGAGGAG 
GTGGAGTCGG AGGAAGAGGA GCGACAACAG CCAGCGGAAG AACCTGTCAG CGTTGCCCAG 
GAAGCAAGAT CTGAACCCAT CTCTCAGGAG GCGGGAGACG AGCAGGAGTC TGAGGAGTCT 
GGAGAAGAAG AGGGAGAGGA GGAGGGCACA GAGTCTGACT TGAGCACAGA GTCCAGTCTG 
AAGAAAAAGT TGAAAAAGAA AACAAAAGCA GACAGCGCGT GGCTCAGGCC GTCCAGGAAA 
CGCAAGAGGA GGCTAAAGGC CAAAGTCTTC TGCTCAGAAC CAGAGGCGGT GGATCAACCT 
CAGGCCTCAG CCCACACCCC GGCAGGCGAT AGGAAGGAGT ACACGCAGAT TCCCTCGCCT 
GAGTCGGTCA ACCTCAACAA GCCCCAAGAG CCGACGGTTT CCTCACAGGT CAAAGACTCT 
CTGGGGTCGG CCATTGAGGA GGCTCAGGAG CTTCCGCTCT GCAGCTGCCG TATGGAGACG 
CCCAAGAGTC GAGAGATTCT CATCCTGGCG GACAGGAAGT GCATGGCCAC AGAGAGCGTG 
GATGGACAGC TGACCCGCTG CCAGGGTGCC ATACTGAAAC ATGAAATGAT GCGTCCCTCC 
AACTCCGTTC AGCTGCTGGT TCTGTGCGAG GACCACCGTA ACGGCATGGT GAAGCACCAG 
TGCTGCCCCG GGTGTGGCTT CTTCTGCAGG GCTGGGACCT TCATGGAGTG CCAACCGGAC 
GTCAGCATCT CTCACCGTTT CCATCGTGCT TGTGCCTCAA TGCTGAAAGG TCAAAGCTTC 
TGTCCCCACT GCGGAGAGGA GGCCAGCAAG GCCAAGGAGG TCACCATCGC CAAGGCCGAC 
ACCACCTCCA CTGTGCCTCC TGGGCTGACA CACGGACCTG CCACCACCGG GGTCCCCGAG 
GGCCGAGCAG ACACCACCAC TGGCAGTTCT TCTCGACTGG CCGTGGGGGC TGAAGTCAGC 
GGGAAGGCCG ACAGTTCGCT GCCCATCCGT TCTGCACATG GACTCGACAC CCCTGCTGTT 
CCCGGGTCAT CTAGGACCGC ACTGCTGCAG GCCGGGACAG GAACAATGGA CTCACCCACA 
ATCCCTCAAG GAACGCAGAG AGAGACTCTA GAGAGCATCC TGGTAGCTCT CGACACAGAG 
AAACCCAAGA AGCTACGTTT TCACCCCAAA CAACTTTACC TCTCAGCCAA ACAGGGAGAA 
CTCAAGAAGG TCTTGCTTAT GTTGGTGGAC GGTATCGACC CAAATTTTAA GATGGAATCT 
CAGAACAAAC GCACACCTCT CCATGCCGCA GCTGAGGGAG GACACAAAGA CATCTGTCAC 
ATGCTCGTAC AAGCTGGTGC GAACTTGGAC ATGTGTGATG AAGATCAGCG GACGCCACTC 
ATGGAGGCGT GTGAGAACAA CCACATGGAG ACTGTCCTGT ACCTGCTGAG AGCTGGAGCA 
AGCGCCACGC ACAAGGATGT TGAAGGGTTC ACGTGTCTCC ACCTAGCGGC AAAGTCTGGT 
CATTACAACA TTGTCGAGCA CCTTCTGTCC ACAGGATTGA TTAACATAAA CTGTCAGGAC 
GATGGCGGTT GGACAGCCAT GATCTGGGCG ACAGAGTACA AGCATGTGGA CCAGGTGAAG 
CTACTTCTGT CCAAAGGAGC TGACATCAGC ATCAGGGACA AGGAAGAGAA TATCTGCCTT 
CACTGGGCAG CATTCTCCGG CAGTGTGGAC ATCGCCGAGC TGCTCCTGAA CGCCCGCTGT 
GATCTGCAGG TCGTCAACAT CCATGGAGAC TCTCCTCTGC ACATCGCTGC CCGGGAGAAT 
CGTCTGGACT GTGTCACACT TTTTCTGTCC CGAGGGGCAG ATGTGTTTCT GAAGAACCGC 
GAGGGAGAAA CTCCTCCAGA CTGCTGCAGC CACAACTCAA AGGCCTGGGC AGCCCTGCAG 
GCCCACAGGA GGGAGAAGGA TGCCAAGAAC AGCAGGCTCA ATCGAACAGA GCAAAAAGTC 
CTTCACAGTG ACATCGCTCT GGGGCAGGAG CGTGTTCCCA TTCCCTGTGT CAACTCTGTC 
GACAGTGAAC CTTATCCAGA CGGCTACAAA TACATCCCCG AAAACTGTGT CACTTCCCCC 
ATGAACATCG ACAGGAACAT CACACACTTG CAGTACTGCG TTTGTAAAGA AGACTGTTCA 
ACAAGTATCT GCATGTGTGG ACAACTCAGT CTGCGCTGCT GGTACGACAA GGCTGGTCGT 
CTTCTACCTG AATTCTGCCG TGAGGAGCCT CCTCTCATCT TTGAATGTAA CCATGCATGC 
TCCTGTTGGA GGACCTGTAA GAACCGTGTA GTGCAAAACG GACTCAGGAC GAGGCTTCAG 
CTCTTCAGGA CCAATAAGAA GGGCTGGGGT GTTCGTGCAG TACAAGACAT ACCACGGGGG 
ACCTTTATAT GCGAGTACGT AGGTGAGATC ATCTCGGAAG CCGAGGCAGA GATGAGGCAG 
AATGACGCTT ACCTGTTCAG TCTGGATGAT AAGCCTCAAG ATCTGTACTG CATCGATGCC 
CGTTTCTATG GAAACATCAG CCGCTTCCTT AATCACATGT GCGAGCCCAA CTTGTTTGCC 
TGTCGAGTGT TCACGACACA CCAGGACCTC CGTTTCCCAC ACATTGCCTT TTTTGCCAGT 
GAAAACATCA CAGCTGGAGA GGAGCTTGGA TTTAACTATG GCGACCACTT CTGGGAAGTC 
AAAAGCAAAC TGTTCAACTG CGAATGTGGC TCTTCTAAGT GCAAGTACTC GTCAGCAGCC 
ATGGCATCGC TGCAGGCGGA TAGCACACCA GAGGACCGGC AGCAGCCCAG CGCGTCACCT 
GACACCAGCT CTTCCAACAG TCCTTCCAGT CCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027134414.1
CDS	106..4044
Translation	MPAGLAKGSVDKGASTKKEELNAASNGEEKPDGDEEVAARLASTAAEAMLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQEAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKVFCSEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_027278613.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027278613.1

ORF Insert Sequence:

ATGCCCGCTG GTCTAGCCAA GGGCAGTGTG GATAAGGGTG CCTCCACAAA AAAAGAGGAA 
CTCAATGCAG CAAGCAACGG AGAGGAGAAG CCAGATGGGG ACGAAGAGGT AGCAGCCAGA 
CTGGCCTCCA CTGCAGCAGA GGCAATGCTA AACGGCACTG AATGTGACGA CGCGAGGCAC 
AAGAGCCCGA CACACGGCTC TGCAACGGGC AGCAAGAAGA CTTTACTGTT GGTTAACGAA 
AACGGCACGT CAGACACAGA GCCACCGCAC GGTTCTGTCA CTGGAAGCAA TGGGTTCATT 
CTCACTAAGC AGCAGCAGGA AGATGGCTCT GCAGCGACGC CGGGTTTGGG AGTTTCCCCT 
CATAGGACTA ACTGGTTGCC TTCGGGCTCA CCAGCAGGGG GGAGCACAGC CAAACCCCTC 
CCTGCCTTGG CGTCTGGGTG TGCACAGAGT TTAGGTGCAC TAAGGACTGA CCCCAGCATG 
GGGACTACAT CGGGACAAGG GACACCAGAC ACTAAAAATG GCACAGCACC AGCTCCAACA 
CCGGCCCCAG CACCCGTCAC GATACACAGA GCGCGCAAGA CCATGTCCAG ACCTGCTGTC 
AGTGCGGCAC AAAAGCTTCT TAACAGGGAA TTAAGAGAAG CAAAGAGTGC CAAAATGGAG 
AAGACGGAGG CTCATGTTGC ACCCGACGCC CAGAAGTCCT CGTCGCAGTC CTCCGCTCAG 
AACCACCTAC CTCAGAGTCC ACCGGATACG CCAGCTTCAG CACAAACGGC TTCATCAGCT 
CCACCAACGC CTCCAGCGCC TCCACCACCA CCCCTGGCTC CCGCACCACA CTCTGCTCCT 
CCAGCTCCGG CCAAACTCCA CCTAGGCTCT TACTCAGGAG GGTTGTCATC CCGTAAGAAG 
AAAAGGAGGA TGGGAACGTA CAGTCTGGTT CCCAAGAAGA AAGCCAAAGT GCTCAGGCAG 
AGAACTATGA TGGAAATGTA TAAGGATTTA CGACACTCCA AGAGTCCACA GACTAAAGAG 
GTATCAAACA TAAATGGGGA GAAGGTTGAA AATGCATCAG AGGAGGAGGA GTCCGAGGAG 
GTGGAGTCGG AGGAAGAGGA GCGACAACAG CCAGCGGAAG AACCTGTCAG CGTTGCCCAG 
GAAGCAAGAT CTGAACCCAT CTCTCAGGAG GCGGGAGACG AGCAGGAGTC TGAGGAGTCT 
GGAGAAGAAG AGGGAGAGGA GGAGGGCACA GAGTCTGACT TGAGCACAGA GTCCAGTCTG 
AAGAAAAAGT TGAAAAAGAA AACAAAAGCA GACAGCGCGT GGCTCAGGCC GTCCAGGAAA 
CGCAAGAGGA GGCTAAAGGC CAAAGTCTTC TGCTCAGAAC CAGAGGCGGT GGATCAACCT 
CAGGCCTCAG CCCACACCCC GGCAGGCGAT AGGAAGGAGT ACACGCAGAT TCCCTCGCCT 
GAGTCGGTCA ACCTCAACAA GCCCCAAGAG CCGACGGTTT CCTCACAGGT CAAAGACTCT 
CTGGGGTCGG CCATTGAGGA GGCTCAGGAG CTTCCGCTCT GCAGCTGCCG TATGGAGACG 
CCCAAGAGTC GAGAGATTCT CATCCTGGCG GACAGGAAGT GCATGGCCAC AGAGAGCGTG 
GATGGACAGC TGACCCGCTG CCAGGGTGCC ATACTGAAAC ATGAAATGAT GCGTCCCTCC 
AACTCCGTTC AGCTGCTGGT TCTGTGCGAG GACCACCGTA ACGGCATGGT GAAGCACCAG 
TGCTGCCCCG GGTGTGGCTT CTTCTGCAGG GCTGGGACCT TCATGGAGTG CCAACCGGAC 
GTCAGCATCT CTCACCGTTT CCATCGTGCT TGTGCCTCAA TGCTGAAAGG TCAAAGCTTC 
TGTCCCCACT GCGGAGAGGA GGCCAGCAAG GCCAAGGAGG TCACCATCGC CAAGGCCGAC 
ACCACCTCCA CTGTGCCTCC TGGGCTGACA CACGGACCTG CCACCACCGG GGTCCCCGAG 
GGCCGAGCAG ACACCACCAC TGGCAGTTCT TCTCGACTGG CCGTGGGGGC TGAAGTCAGC 
GGGAAGGCCG ACAGTTCGCT GCCCATCCGT TCTGCACATG GACTCGACAC CCCTGCTGTT 
CCCGGGTCAT CTAGGACCGC ACTGCTGCAG GCCGGGACAG GAACAATGGA CTCACCCACA 
ATCCCTCAAG GAACGCAGAG AGAGACTCTA GAGAGCATCC TGGTAGCTCT CGACACAGAG 
AAACCCAAGA AGCTACGTTT TCACCCCAAA CAACTTTACC TCTCAGCCAA ACAGGGAGAA 
CTCAAGAAGG TCTTGCTTAT GTTGGTGGAC GGTATCGACC CAAATTTTAA GATGGAATCT 
CAGAACAAAC GCACACCTCT CCATGCCGCA GCTGAGGGAG GACACAAAGA CATCTGTCAC 
ATGCTCGTAC AAGCTGGTGC GAACTTGGAC ATGTGTGATG AAGATCAGCG GACGCCACTC 
ATGGAGGCGT GTGAGAACAA CCACATGGAG ACTGTCCTGT ACCTGCTGAG AGCTGGAGCA 
AGCGCCACGC ACAAGGATGT TGAAGGGTTC ACGTGTCTCC ACCTAGCGGC AAAGTCTGGT 
CATTACAACA TTGTCGAGCA CCTTCTGTCC ACAGGATTGA TTAACATAAA CTGTCAGGAC 
GATGGCGGTT GGACAGCCAT GATCTGGGCG ACAGAGTACA AGCATGTGGA CCAGGTGAAG 
CTACTTCTGT CCAAAGGAGC TGACATCAGC ATCAGGGACA AGGAAGAGAA TATCTGCCTT 
CACTGGGCAG CATTCTCCGG CAGTGTGGAC ATCGCCGAGC TGCTCCTGAA CGCCCGCTGT 
GATCTGCAGG TCGTCAACAT CCATGGAGAC TCTCCTCTGC ACATCGCTGC CCGGGAGAAT 
CGTCTGGACT GTGTCACACT TTTTCTGTCC CGAGGGGCAG ATGTGTTTCT GAAGAACCGC 
GAGGGAGAAA CTCCTCCAGA CTGCTGCAGC CACAACTCAA AGGCCTGGGC AGCCCTGCAG 
GCCCACAGGA GGGAGAAGGA TGCCAAGAAC AGCAGGCTCA ATCGAACAGA GCAAAAAGTC 
CTTCACAGTG ACATCGCTCT GGGGCAGGAG CGTGTTCCCA TTCCCTGTGT CAACTCTGTC 
GACAGTGAAC CTTATCCAGA CGGCTACAAA TACATCCCCG AAAACTGTGT CACTTCCCCC 
ATGAACATCG ACAGGAACAT CACACACTTG CAGTACTGCG TTTGTAAAGA AGACTGTTCA 
ACAAGTATCT GCATGTGTGG ACAACTCAGT CTGCGCTGCT GGTACGACAA GGCTGGTCGT 
CTTCTACCTG AATTCTGCCG TGAGGAGCCT CCTCTCATCT TTGAATGTAA CCATGCATGC 
TCCTGTTGGA GGACCTGTAA GAACCGTGTA GTGCAAAACG GACTCAGGAC GAGGCTTCAG 
CTCTTCAGGA CCAATAAGAA GGGCTGGGGT GTTCGTGCAG TACAAGACAT ACCACGGGGG 
ACCTTTATAT GCGAGTACGT AGGTGAGATC ATCTCGGAAG CCGAGGCAGA GATGAGGCAG 
AATGACGCTT ACCTGTTCAG TCTGGATGAT AAGCCTCAAG ATCTGTACTG CATCGATGCC 
CGTTTCTATG GAAACATCAG CCGCTTCCTT AATCACATGT GCGAGCCCAA CTTGTTTGCC 
TGTCGAGTGT TCACGACACA CCAGGACCTC CGTTTCCCAC ACATTGCCTT TTTTGCCAGT 
GAAAACATCA CAGCTGGAGA GGAGCTTGGA TTTAACTATG GCGACCACTT CTGGGAAGTC 
AAAAGCAAAC TGTTCAACTG CGAATGTGGC TCTTCTAAGT GCAAGTACTC GTCAGCAGCC 
ATGGCATCGC TGCAGGCGGA TAGCACACCA GAGGACCGGC AGCAGCCCAG CGCGTCACCT 
GACACCAGCT CTTCCAACAG TCCTTCCAGT CCTTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104922882 ( XM_027278614.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027134415.1 Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X6, mRNA.

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CloneID	OLa219527
Clone ID Related Accession (Same CDS sequence)	XM_027278614.1
Accession Version	XM_027278614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	histone-lysine N-methyltransferase EHMT1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040014.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAACG GCACTGAATG TGACGACGCG AGGCACAAGA GCCCGACACA CGGCTCTGCA 
ACGGGCAGCA AGAAGACTTT ACTGTTGGTT AACGAAAACG GCACGTCAGA CACAGAGCCA 
CCGCACGGTT CTGTCACTGG AAGCAATGGG TTCATTCTCA CTAAGCAGCA GCAGGAAGAT 
GGCTCTGCAG CGACGCCGGG TTTGGGAGTT TCCCCTCATA GGACTAACTG GTTGCCTTCG 
GGCTCACCAG CAGGGGGGAG CACAGCCAAA CCCCTCCCTG CCTTGGCGTC TGGGTGTGCA 
CAGAGTTTAG GTGCACTAAG GACTGACCCC AGCATGGGGA CTACATCGGG ACAAGGGACA 
CCAGACACTA AAAATGGCAC AGCACCAGCT CCAACACCGG CCCCAGCACC CGTCACGATA 
CACAGAGCGC GCAAGACCAT GTCCAGACCT GCTGTCAGTG CGGCACAAAA GCTTCTTAAC 
AGGGAATTAA GAGAAGCAAA GAGTGCCAAA ATGGAGAAGA CGGAGGCTCA TGTTGCACCC 
GACGCCCAGA AGTCCTCGTC GCAGTCCTCC GCTCAGAACC ACCTACCTCA GAGTCCACCG 
GATACGCCAG CTTCAGCACA AACGGCTTCA TCAGCTCCAC CAACGCCTCC AGCGCCTCCA 
CCACCACCCC TGGCTCCCGC ACCACACTCT GCTCCTCCAG CTCCGGCCAA ACTCCACCTA 
GGCTCTTACT CAGGAGGGTT GTCATCCCGT AAGAAGAAAA GGAGGATGGG AACGTACAGT 
CTGGTTCCCA AGAAGAAAGC CAAAGTGCTC AGGCAGAGAA CTATGATGGA AATGTATAAG 
GATTTACGAC ACTCCAAGAG TCCACAGACT AAAGAGGTAT CAAACATAAA TGGGGAGAAG 
GTTGAAAATG CATCAGAGGA GGAGGAGTCC GAGGAGGTGG AGTCGGAGGA AGAGGAGCGA 
CAACAGCCAG CGGAAGAACC TGTCAGCGTT GCCCAGGAAG CAAGATCTGA ACCCATCTCT 
CAGGAGGCGG GAGACGAGCA GGAGTCTGAG GAGTCTGGAG AAGAAGAGGG AGAGGAGGAG 
GGCACAGAGT CTGACTTGAG CACAGAGTCC AGTCTGAAGA AAAAGTTGAA AAAGAAAACA 
AAAGCAGACA GCGCGTGGCT CAGGCCGTCC AGGAAACGCA AGAGGAGGCT AAAGGCCAAA 
GTCTTCTGCT CAGAACCAGA GGCGGTGGAT CAACCTCAGG CCTCAGCCCA CACCCCGGCA 
GGCGATAGGA AGGAGTACAC GCAGATTCCC TCGCCTGAGT CGGTCAACCT CAACAAGCCC 
CAAGAGCCGA CGGTTTCCTC ACAGGTCAAA GACTCTCTGG GGTCGGCCAT TGAGGAGGCT 
CAGGAGCTTC CGCTCTGCAG CTGCCGTATG GAGACGCCCA AGAGTCGAGA GATTCTCATC 
CTGGCGGACA GGAAGTGCAT GGCCACAGAG AGCGTGGATG GACAGCTGAC CCGCTGCCAG 
GGTGCCATAC TGAAACATGA AATGATGCGT CCCTCCAACT CCGTTCAGCT GCTGGTTCTG 
TGCGAGGACC ACCGTAACGG CATGGTGAAG CACCAGTGCT GCCCCGGGTG TGGCTTCTTC 
TGCAGGGCTG GGACCTTCAT GGAGTGCCAA CCGGACGTCA GCATCTCTCA CCGTTTCCAT 
CGTGCTTGTG CCTCAATGCT GAAAGGTCAA AGCTTCTGTC CCCACTGCGG AGAGGAGGCC 
AGCAAGGCCA AGGAGGTCAC CATCGCCAAG GCCGACACCA CCTCCACTGT GCCTCCTGGG 
CTGACACACG GACCTGCCAC CACCGGGGTC CCCGAGGGCC GAGCAGACAC CACCACTGGC 
AGTTCTTCTC GACTGGCCGT GGGGGCTGAA GTCAGCGGGA AGGCCGACAG TTCGCTGCCC 
ATCCGTTCTG CACATGGACT CGACACCCCT GCTGTTCCCG GGTCATCTAG GACCGCACTG 
CTGCAGGCCG GGACAGGAAC AATGGACTCA CCCACAATCC CTCAAGGAAC GCAGAGAGAG 
ACTCTAGAGA GCATCCTGGT AGCTCTCGAC ACAGAGAAAC CCAAGAAGCT ACGTTTTCAC 
CCCAAACAAC TTTACCTCTC AGCCAAACAG GGAGAACTCA AGAAGGTCTT GCTTATGTTG 
GTGGACGGTA TCGACCCAAA TTTTAAGATG GAATCTCAGA ACAAACGCAC ACCTCTCCAT 
GCCGCAGCTG AGGGAGGACA CAAAGACATC TGTCACATGC TCGTACAAGC TGGTGCGAAC 
TTGGACATGT GTGATGAAGA TCAGCGGACG CCACTCATGG AGGCGTGTGA GAACAACCAC 
ATGGAGACTG TCCTGTACCT GCTGAGAGCT GGAGCAAGCG CCACGCACAA GGATGTTGAA 
GGGTTCACGT GTCTCCACCT AGCGGCAAAG TCTGGTCATT ACAACATTGT CGAGCACCTT 
CTGTCCACAG GATTGATTAA CATAAACTGT CAGGACGATG GCGGTTGGAC AGCCATGATC 
TGGGCGACAG AGTACAAGCA TGTGGACCAG GTGAAGCTAC TTCTGTCCAA AGGAGCTGAC 
ATCAGCATCA GGGACAAGGA AGAGAATATC TGCCTTCACT GGGCAGCATT CTCCGGCAGT 
GTGGACATCG CCGAGCTGCT CCTGAACGCC CGCTGTGATC TGCAGGTCGT CAACATCCAT 
GGAGACTCTC CTCTGCACAT CGCTGCCCGG GAGAATCGTC TGGACTGTGT CACACTTTTT 
CTGTCCCGAG GGGCAGATGT GTTTCTGAAG AACCGCGAGG GAGAAACTCC TCCAGACTGC 
TGCAGCCACA ACTCAAAGGC CTGGGCAGCC CTGCAGGCCC ACAGGAGGGA GAAGGATGCC 
AAGAACAGCA GGCTCAATCG AACAGAGCAA AAAGTCCTTC ACAGTGACAT CGCTCTGGGG 
CAGGAGCGTG TTCCCATTCC CTGTGTCAAC TCTGTCGACA GTGAACCTTA TCCAGACGGC 
TACAAATACA TCCCCGAAAA CTGTGTCACT TCCCCCATGA ACATCGACAG GAACATCACA 
CACTTGCAGT ACTGCGTTTG TAAAGAAGAC TGTTCAACAA GTATCTGCAT GTGTGGACAA 
CTCAGTCTGC GCTGCTGGTA CGACAAGGCT GGTCGTCTTC TACCTGAATT CTGCCGTGAG 
GAGCCTCCTC TCATCTTTGA ATGTAACCAT GCATGCTCCT GTTGGAGGAC CTGTAAGAAC 
CGTGTAGTGC AAAACGGACT CAGGACGAGG CTTCAGCTCT TCAGGACCAA TAAGAAGGGC 
TGGGGTGTTC GTGCAGTACA AGACATACCA CGGGGGACCT TTATATGCGA GTACGTAGGT 
GAGATCATCT CGGAAGCCGA GGCAGAGATG AGGCAGAATG ACGCTTACCT GTTCAGTCTG 
GATGATAAGC CTCAAGATCT GTACTGCATC GATGCCCGTT TCTATGGAAA CATCAGCCGC 
TTCCTTAATC ACATGTGCGA GCCCAACTTG TTTGCCTGTC GAGTGTTCAC GACACACCAG 
GACCTCCGTT TCCCACACAT TGCCTTTTTT GCCAGTGAAA ACATCACAGC TGGAGAGGAG 
CTTGGATTTA ACTATGGCGA CCACTTCTGG GAAGTCAAAA GCAAACTGTT CAACTGCGAA 
TGTGGCTCTT CTAAGTGCAA GTACTCGTCA GCAGCCATGG CATCGCTGCA GGCGGATAGC 
ACACCAGAGG ACCGGCAGCA GCCCAGCGCG TCACCTGACA CCAGCTCTTC CAACAGTCCT 
TCCAGTCCTT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027134415.1
CDS	162..3956
Translation	MLNGTECDDARHKSPTHGSATGSKKTLLLVNENGTSDTEPPHGSVTGSNGFILTKQQQEDGSAATPGLGVSPHRTNWLPSGSPAGGSTAKPLPALASGCAQSLGALRTDPSMGTTSGQGTPDTKNGTAPAPTPAPAPVTIHRARKTMSRPAVSAAQKLLNRELREAKSAKMEKTEAHVAPDAQKSSSQSSAQNHLPQSPPDTPASAQTASSAPPTPPAPPPPPLAPAPHSAPPAPAKLHLGSYSGGLSSRKKKRRMGTYSLVPKKKAKVLRQRTMMEMYKDLRHSKSPQTKEVSNINGEKVENASEEEESEEVESEEEERQQPAEEPVSVAQEARSEPISQEAGDEQESEESGEEEGEEEGTESDLSTESSLKKKLKKKTKADSAWLRPSRKRKRRLKAKVFCSEPEAVDQPQASAHTPAGDRKEYTQIPSPESVNLNKPQEPTVSSQVKDSLGSAIEEAQELPLCSCRMETPKSREILILADRKCMATESVDGQLTRCQGAILKHEMMRPSNSVQLLVLCEDHRNGMVKHQCCPGCGFFCRAGTFMECQPDVSISHRFHRACASMLKGQSFCPHCGEEASKAKEVTIAKADTTSTVPPGLTHGPATTGVPEGRADTTTGSSSRLAVGAEVSGKADSSLPIRSAHGLDTPAVPGSSRTALLQAGTGTMDSPTIPQGTQRETLESILVALDTEKPKKLRFHPKQLYLSAKQGELKKVLLMLVDGIDPNFKMESQNKRTPLHAAAEGGHKDICHMLVQAGANLDMCDEDQRTPLMEACENNHMETVLYLLRAGASATHKDVEGFTCLHLAAKSGHYNIVEHLLSTGLININCQDDGGWTAMIWATEYKHVDQVKLLLSKGADISIRDKEENICLHWAAFSGSVDIAELLLNARCDLQVVNIHGDSPLHIAARENRLDCVTLFLSRGADVFLKNREGETPPDCCSHNSKAWAALQAHRREKDAKNSRLNRTEQKVLHSDIALGQERVPIPCVNSVDSEPYPDGYKYIPENCVTSPMNIDRNITHLQYCVCKEDCSTSICMCGQLSLRCWYDKAGRLLPEFCREEPPLIFECNHACSCWRTCKNRVVQNGLRTRLQLFRTNKKGWGVRAVQDIPRGTFICEYVGEIISEAEAEMRQNDAYLFSLDDKPQDLYCIDARFYGNISRFLNHMCEPNLFACRVFTTHQDLRFPHIAFFASENITAGEELGFNYGDHFWEVKSKLFNCECGSSKCKYSSAAMASLQADSTPEDRQQPSASPDTSSSNSPSSPS

Target ORF information:

RefSeq Version	XM_027278614.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea histone-lysine N-methyltransferase EHMT1 (LOC104922882), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027278614.1

ORF Insert Sequence:

ATGCTAAACG GCACTGAATG TGACGACGCG AGGCACAAGA GCCCGACACA CGGCTCTGCA 
ACGGGCAGCA AGAAGACTTT ACTGTTGGTT AACGAAAACG GCACGTCAGA CACAGAGCCA 
CCGCACGGTT CTGTCACTGG AAGCAATGGG TTCATTCTCA CTAAGCAGCA GCAGGAAGAT 
GGCTCTGCAG CGACGCCGGG TTTGGGAGTT TCCCCTCATA GGACTAACTG GTTGCCTTCG 
GGCTCACCAG CAGGGGGGAG CACAGCCAAA CCCCTCCCTG CCTTGGCGTC TGGGTGTGCA 
CAGAGTTTAG GTGCACTAAG GACTGACCCC AGCATGGGGA CTACATCGGG ACAAGGGACA 
CCAGACACTA AAAATGGCAC AGCACCAGCT CCAACACCGG CCCCAGCACC CGTCACGATA 
CACAGAGCGC GCAAGACCAT GTCCAGACCT GCTGTCAGTG CGGCACAAAA GCTTCTTAAC 
AGGGAATTAA GAGAAGCAAA GAGTGCCAAA ATGGAGAAGA CGGAGGCTCA TGTTGCACCC 
GACGCCCAGA AGTCCTCGTC GCAGTCCTCC GCTCAGAACC ACCTACCTCA GAGTCCACCG 
GATACGCCAG CTTCAGCACA AACGGCTTCA TCAGCTCCAC CAACGCCTCC AGCGCCTCCA 
CCACCACCCC TGGCTCCCGC ACCACACTCT GCTCCTCCAG CTCCGGCCAA ACTCCACCTA 
GGCTCTTACT CAGGAGGGTT GTCATCCCGT AAGAAGAAAA GGAGGATGGG AACGTACAGT 
CTGGTTCCCA AGAAGAAAGC CAAAGTGCTC AGGCAGAGAA CTATGATGGA AATGTATAAG 
GATTTACGAC ACTCCAAGAG TCCACAGACT AAAGAGGTAT CAAACATAAA TGGGGAGAAG 
GTTGAAAATG CATCAGAGGA GGAGGAGTCC GAGGAGGTGG AGTCGGAGGA AGAGGAGCGA 
CAACAGCCAG CGGAAGAACC TGTCAGCGTT GCCCAGGAAG CAAGATCTGA ACCCATCTCT 
CAGGAGGCGG GAGACGAGCA GGAGTCTGAG GAGTCTGGAG AAGAAGAGGG AGAGGAGGAG 
GGCACAGAGT CTGACTTGAG CACAGAGTCC AGTCTGAAGA AAAAGTTGAA AAAGAAAACA 
AAAGCAGACA GCGCGTGGCT CAGGCCGTCC AGGAAACGCA AGAGGAGGCT AAAGGCCAAA 
GTCTTCTGCT CAGAACCAGA GGCGGTGGAT CAACCTCAGG CCTCAGCCCA CACCCCGGCA 
GGCGATAGGA AGGAGTACAC GCAGATTCCC TCGCCTGAGT CGGTCAACCT CAACAAGCCC 
CAAGAGCCGA CGGTTTCCTC ACAGGTCAAA GACTCTCTGG GGTCGGCCAT TGAGGAGGCT 
CAGGAGCTTC CGCTCTGCAG CTGCCGTATG GAGACGCCCA AGAGTCGAGA GATTCTCATC 
CTGGCGGACA GGAAGTGCAT GGCCACAGAG AGCGTGGATG GACAGCTGAC CCGCTGCCAG 
GGTGCCATAC TGAAACATGA AATGATGCGT CCCTCCAACT CCGTTCAGCT GCTGGTTCTG 
TGCGAGGACC ACCGTAACGG CATGGTGAAG CACCAGTGCT GCCCCGGGTG TGGCTTCTTC 
TGCAGGGCTG GGACCTTCAT GGAGTGCCAA CCGGACGTCA GCATCTCTCA CCGTTTCCAT 
CGTGCTTGTG CCTCAATGCT GAAAGGTCAA AGCTTCTGTC CCCACTGCGG AGAGGAGGCC 
AGCAAGGCCA AGGAGGTCAC CATCGCCAAG GCCGACACCA CCTCCACTGT GCCTCCTGGG 
CTGACACACG GACCTGCCAC CACCGGGGTC CCCGAGGGCC GAGCAGACAC CACCACTGGC 
AGTTCTTCTC GACTGGCCGT GGGGGCTGAA GTCAGCGGGA AGGCCGACAG TTCGCTGCCC 
ATCCGTTCTG CACATGGACT CGACACCCCT GCTGTTCCCG GGTCATCTAG GACCGCACTG 
CTGCAGGCCG GGACAGGAAC AATGGACTCA CCCACAATCC CTCAAGGAAC GCAGAGAGAG 
ACTCTAGAGA GCATCCTGGT AGCTCTCGAC ACAGAGAAAC CCAAGAAGCT ACGTTTTCAC 
CCCAAACAAC TTTACCTCTC AGCCAAACAG GGAGAACTCA AGAAGGTCTT GCTTATGTTG 
GTGGACGGTA TCGACCCAAA TTTTAAGATG GAATCTCAGA ACAAACGCAC ACCTCTCCAT 
GCCGCAGCTG AGGGAGGACA CAAAGACATC TGTCACATGC TCGTACAAGC TGGTGCGAAC 
TTGGACATGT GTGATGAAGA TCAGCGGACG CCACTCATGG AGGCGTGTGA GAACAACCAC 
ATGGAGACTG TCCTGTACCT GCTGAGAGCT GGAGCAAGCG CCACGCACAA GGATGTTGAA 
GGGTTCACGT GTCTCCACCT AGCGGCAAAG TCTGGTCATT ACAACATTGT CGAGCACCTT 
CTGTCCACAG GATTGATTAA CATAAACTGT CAGGACGATG GCGGTTGGAC AGCCATGATC 
TGGGCGACAG AGTACAAGCA TGTGGACCAG GTGAAGCTAC TTCTGTCCAA AGGAGCTGAC 
ATCAGCATCA GGGACAAGGA AGAGAATATC TGCCTTCACT GGGCAGCATT CTCCGGCAGT 
GTGGACATCG CCGAGCTGCT CCTGAACGCC CGCTGTGATC TGCAGGTCGT CAACATCCAT 
GGAGACTCTC CTCTGCACAT CGCTGCCCGG GAGAATCGTC TGGACTGTGT CACACTTTTT 
CTGTCCCGAG GGGCAGATGT GTTTCTGAAG AACCGCGAGG GAGAAACTCC TCCAGACTGC 
TGCAGCCACA ACTCAAAGGC CTGGGCAGCC CTGCAGGCCC ACAGGAGGGA GAAGGATGCC 
AAGAACAGCA GGCTCAATCG AACAGAGCAA AAAGTCCTTC ACAGTGACAT CGCTCTGGGG 
CAGGAGCGTG TTCCCATTCC CTGTGTCAAC TCTGTCGACA GTGAACCTTA TCCAGACGGC 
TACAAATACA TCCCCGAAAA CTGTGTCACT TCCCCCATGA ACATCGACAG GAACATCACA 
CACTTGCAGT ACTGCGTTTG TAAAGAAGAC TGTTCAACAA GTATCTGCAT GTGTGGACAA 
CTCAGTCTGC GCTGCTGGTA CGACAAGGCT GGTCGTCTTC TACCTGAATT CTGCCGTGAG 
GAGCCTCCTC TCATCTTTGA ATGTAACCAT GCATGCTCCT GTTGGAGGAC CTGTAAGAAC 
CGTGTAGTGC AAAACGGACT CAGGACGAGG CTTCAGCTCT TCAGGACCAA TAAGAAGGGC 
TGGGGTGTTC GTGCAGTACA AGACATACCA CGGGGGACCT TTATATGCGA GTACGTAGGT 
GAGATCATCT CGGAAGCCGA GGCAGAGATG AGGCAGAATG ACGCTTACCT GTTCAGTCTG 
GATGATAAGC CTCAAGATCT GTACTGCATC GATGCCCGTT TCTATGGAAA CATCAGCCGC 
TTCCTTAATC ACATGTGCGA GCCCAACTTG TTTGCCTGTC GAGTGTTCAC GACACACCAG 
GACCTCCGTT TCCCACACAT TGCCTTTTTT GCCAGTGAAA ACATCACAGC TGGAGAGGAG 
CTTGGATTTA ACTATGGCGA CCACTTCTGG GAAGTCAAAA GCAAACTGTT CAACTGCGAA 
TGTGGCTCTT CTAAGTGCAA GTACTCGTCA GCAGCCATGG CATCGCTGCA GGCGGATAGC 
ACACCAGAGG ACCGGCAGCA GCCCAGCGCG TCACCTGACA CCAGCTCTTC CAACAGTCCT 
TCCAGTCCTT CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LOC104922882
Entrez Gene ID	104922882
Full Name	histone-lysine N-methyltransferase EHMT1
Synonyms	ehmt1
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC104922882 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene

Clones

gRNAs

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