LCAT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LCAT
Entrez Gene ID	3931
Full Name	lecithin-cholesterol acyltransferase
General protein information	Preferred Names lecithin-cholesterol acyltransferase Names phosphatidylcholine-sterol acyltransferase phosphatidylcholine--sterol O-acyltransferase phospholipid-cholesterol acyltransferase testicular secretory protein Li 24
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008].
Disorder MIM:	606967
Disorder Html:	Norum disease, 245900 (3); Fish-eye disease, 136120 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000229.1	NP_000220.1	phosphatidylcholine-sterol acyltransferase precursor
NM_000229.2	NP_000220.1	phosphatidylcholine-sterol acyltransferase precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LCAT	XP_414027.4
Homo sapiens (human)	LCAT	NP_000220.1
Pan troglodytes (chimpanzee)	LCAT	XP_001166500.1
Mus musculus (house mouse)	Lcat	NP_032516.2
Canis lupus familiaris (dog)	LCAT	XP_851673.1
Danio rerio (zebrafish)	LOC793137	XP_001332828.3
Xenopus tropicalis (tropical clawed frog)	lcat	NP_001005715.1
Bos taurus (cattle)	LCAT	NP_001039534.1
Rattus norvegicus (Norway rat)	Lcat	NP_058720.2

Gene Ontology

The following LCAT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LCAT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24761	NM_000229.2 Latest version!	Homo sapiens lecithin-cholesterol acyltransferase (LCAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$184.50-$258.30 $369.00
View Old Accession Versions >>
OHu24761	NM_000229.1	Homo sapiens lecithin-cholesterol acyltransferase (LCAT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$184.50-$258.30 $369.00
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LCAT ( NM_000229.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_000220.1 Homo sapiens lecithin-cholesterol acyltransferase (LCAT), mRNA.

Price & Availability↑

CloneID	OHu24761
Clone ID Related Accession (Same CDS sequence)	NM_000229.1 , NM_000229.2
Accession Version	NM_000229.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	phosphatidylcholine-sterol acyltransferase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M12625.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005564.1, M12625.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCCGC CCGGCTCCCC ATGGCAGTGG GTGACGCTGC TGCTGGGGCT GCTGCTCCCT 
CCTGCCGCCC CCTTCTGGCT CCTCAATGTG CTCTTCCCCC CGCACACCAC GCCCAAGGCT 
GAGCTCAGTA ACCACACACG GCCCGTCATC CTCGTGCCCG GCTGCCTGGG GAATCAGCTA 
GAAGCCAAGC TGGACAAACC AGATGTGGTG AACTGGATGT GCTACCGCAA GACAGAGGAC 
TTCTTCACCA TCTGGCTGGA TCTCAACATG TTCCTACCCC TTGGGGTAGA CTGCTGGATC 
GATAACACCA GGGTTGTCTA CAACCGGAGC TCTGGGCTCG TGTCCAACGC CCCTGGTGTC 
CAGATCCGCG TCCCTGGCTT TGGCAAGACC TACTCTGTGG AGTACCTGGA CAGCAGCAAG 
CTGGCAGGGT ACCTGCACAC ACTGGTGCAG AACCTGGTCA ACAATGGCTA CGTGCGGGAC 
GAGACTGTGC GCGCCGCCCC CTATGACTGG CGGCTGGAGC CCGGCCAGCA GGAGGAGTAC 
TACCGCAAGC TCGCAGGGCT GGTGGAGGAG ATGCACGCTG CCTATGGGAA GCCTGTCTTC 
CTCATTGGCC ACAGCCTCGG CTGTCTACAC TTGCTCTATT TCCTGCTGCG CCAGCCCCAG 
GCCTGGAAGG ACCGCTTTAT TGATGGCTTC ATCTCTCTTG GGGCTCCCTG GGGTGGCTCC 
ATCAAGCCCA TGCTGGTCTT GGCCTCAGGT GACAACCAGG GCATCCCCAT CATGTCCAGC 
ATCAAGCTGA AAGAGGAGCA GCGCATAACC ACCACCTCCC CCTGGATGTT TCCCTCTCGC 
ATGGCGTGGC CTGAGGACCA CGTGTTCATT TCCACACCCA GCTTCAACTA CACAGGCCGT 
GACTTCCAAC GCTTCTTTGC AGACCTGCAC TTTGAGGAAG GCTGGTACAT GTGGCTGCAG 
TCACGTGACC TCCTGGCAGG ACTCCCAGCA CCTGGTGTGG AAGTATACTG TCTTTACGGC 
GTGGGCCTGC CCACGCCCCG CACCTACATC TACGACCACG GCTTCCCCTA CACGGACCCT 
GTGGGTGTGC TCTATGAGGA TGGTGATGAC ACGGTGGCGA CCCGCAGCAC CGAGCTCTGT 
GGCCTGTGGC AGGGCCGCCA GCCACAGCCT GTGCACCTGC TGCCCCTGCA CGGGATACAG 
CATCTCAACA TGGTCTTCAG CAACCTGACC CTGGAGCACA TCAATGCCAT CCTGCTGGGT 
GCCTACCGCC AGGGTCCCCC TGCATCCCCG ACTGCCAGCC CAGAGCCCCC GCCTCCTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000220.1
CDS	12..1334
Translation	MGPPGSPWQWVTLLLGLLLPPAAPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE

Target ORF information:

RefSeq Version	NM_000229.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lecithin-cholesterol acyltransferase (LCAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000229.1

ORF Insert Sequence:

ATGGGGCCGC CCGGCTCCCC ATGGCAGTGG GTGACGCTGC TGCTGGGGCT GCTGCTCCCT 
CCTGCCGCCC CCTTCTGGCT CCTCAATGTG CTCTTCCCCC CGCACACCAC GCCCAAGGCT 
GAGCTCAGTA ACCACACACG GCCCGTCATC CTCGTGCCCG GCTGCCTGGG GAATCAGCTA 
GAAGCCAAGC TGGACAAACC AGATGTGGTG AACTGGATGT GCTACCGCAA GACAGAGGAC 
TTCTTCACCA TCTGGCTGGA TCTCAACATG TTCCTACCCC TTGGGGTAGA CTGCTGGATC 
GATAACACCA GGGTTGTCTA CAACCGGAGC TCTGGGCTCG TGTCCAACGC CCCTGGTGTC 
CAGATCCGCG TCCCTGGCTT TGGCAAGACC TACTCTGTGG AGTACCTGGA CAGCAGCAAG 
CTGGCAGGGT ACCTGCACAC ACTGGTGCAG AACCTGGTCA ACAATGGCTA CGTGCGGGAC 
GAGACTGTGC GCGCCGCCCC CTATGACTGG CGGCTGGAGC CCGGCCAGCA GGAGGAGTAC 
TACCGCAAGC TCGCAGGGCT GGTGGAGGAG ATGCACGCTG CCTATGGGAA GCCTGTCTTC 
CTCATTGGCC ACAGCCTCGG CTGTCTACAC TTGCTCTATT TCCTGCTGCG CCAGCCCCAG 
GCCTGGAAGG ACCGCTTTAT TGATGGCTTC ATCTCTCTTG GGGCTCCCTG GGGTGGCTCC 
ATCAAGCCCA TGCTGGTCTT GGCCTCAGGT GACAACCAGG GCATCCCCAT CATGTCCAGC 
ATCAAGCTGA AAGAGGAGCA GCGCATAACC ACCACCTCCC CCTGGATGTT TCCCTCTCGC 
ATGGCGTGGC CTGAGGACCA CGTGTTCATT TCCACACCCA GCTTCAACTA CACAGGCCGT 
GACTTCCAAC GCTTCTTTGC AGACCTGCAC TTTGAGGAAG GCTGGTACAT GTGGCTGCAG 
TCACGTGACC TCCTGGCAGG ACTCCCAGCA CCTGGTGTGG AAGTATACTG TCTTTACGGC 
GTGGGCCTGC CCACGCCCCG CACCTACATC TACGACCACG GCTTCCCCTA CACGGACCCT 
GTGGGTGTGC TCTATGAGGA TGGTGATGAC ACGGTGGCGA CCCGCAGCAC CGAGCTCTGT 
GGCCTGTGGC AGGGCCGCCA GCCACAGCCT GTGCACCTGC TGCCCCTGCA CGGGATACAG 
CATCTCAACA TGGTCTTCAG CAACCTGACC CTGGAGCACA TCAATGCCAT CCTGCTGGGT 
GCCTACCGCC AGGGTCCCCC TGCATCCCCG ACTGCCAGCC CAGAGCCCCC GCCTCCTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LCAT ( NM_000229.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000220.1 Homo sapiens lecithin-cholesterol acyltransferase (LCAT), mRNA.

Price & Availability↑

CloneID	OHu24761
Clone ID Related Accession (Same CDS sequence)	NM_000229.1 , NM_000229.2
Accession Version	NM_000229.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	phosphatidylcholine-sterol acyltransferase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC040162.5. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_000229.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M12625.1, SRR1803612.27646.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264005.10/ ENSP00000264005.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGGGCCGC CCGGCTCCCC ATGGCAGTGG GTGACGCTGC TGCTGGGGCT GCTGCTCCCT 
CCTGCCGCCC CCTTCTGGCT CCTCAATGTG CTCTTCCCCC CGCACACCAC GCCCAAGGCT 
GAGCTCAGTA ACCACACACG GCCCGTCATC CTCGTGCCCG GCTGCCTGGG GAATCAGCTA 
GAAGCCAAGC TGGACAAACC AGATGTGGTG AACTGGATGT GCTACCGCAA GACAGAGGAC 
TTCTTCACCA TCTGGCTGGA TCTCAACATG TTCCTACCCC TTGGGGTAGA CTGCTGGATC 
GATAACACCA GGGTTGTCTA CAACCGGAGC TCTGGGCTCG TGTCCAACGC CCCTGGTGTC 
CAGATCCGCG TCCCTGGCTT TGGCAAGACC TACTCTGTGG AGTACCTGGA CAGCAGCAAG 
CTGGCAGGGT ACCTGCACAC ACTGGTGCAG AACCTGGTCA ACAATGGCTA CGTGCGGGAC 
GAGACTGTGC GCGCCGCCCC CTATGACTGG CGGCTGGAGC CCGGCCAGCA GGAGGAGTAC 
TACCGCAAGC TCGCAGGGCT GGTGGAGGAG ATGCACGCTG CCTATGGGAA GCCTGTCTTC 
CTCATTGGCC ACAGCCTCGG CTGTCTACAC TTGCTCTATT TCCTGCTGCG CCAGCCCCAG 
GCCTGGAAGG ACCGCTTTAT TGATGGCTTC ATCTCTCTTG GGGCTCCCTG GGGTGGCTCC 
ATCAAGCCCA TGCTGGTCTT GGCCTCAGGT GACAACCAGG GCATCCCCAT CATGTCCAGC 
ATCAAGCTGA AAGAGGAGCA GCGCATAACC ACCACCTCCC CCTGGATGTT TCCCTCTCGC 
ATGGCGTGGC CTGAGGACCA CGTGTTCATT TCCACACCCA GCTTCAACTA CACAGGCCGT 
GACTTCCAAC GCTTCTTTGC AGACCTGCAC TTTGAGGAAG GCTGGTACAT GTGGCTGCAG 
TCACGTGACC TCCTGGCAGG ACTCCCAGCA CCTGGTGTGG AAGTATACTG TCTTTACGGC 
GTGGGCCTGC CCACGCCCCG CACCTACATC TACGACCACG GCTTCCCCTA CACGGACCCT 
GTGGGTGTGC TCTATGAGGA TGGTGATGAC ACGGTGGCGA CCCGCAGCAC CGAGCTCTGT 
GGCCTGTGGC AGGGCCGCCA GCCACAGCCT GTGCACCTGC TGCCCCTGCA CGGGATACAG 
CATCTCAACA TGGTCTTCAG CAACCTGACC CTGGAGCACA TCAATGCCAT CCTGCTGGGT 
GCCTACCGCC AGGGTCCCCC TGCATCCCCG ACTGCCAGCC CAGAGCCCCC GCCTCCTGAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000220.1
CDS	20..1342
Translation	MGPPGSPWQWVTLLLGLLLPPAAPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE

Target ORF information:

RefSeq Version	NM_000229.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lecithin-cholesterol acyltransferase (LCAT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000229.2

ORF Insert Sequence:

ATGGGGCCGC CCGGCTCCCC ATGGCAGTGG GTGACGCTGC TGCTGGGGCT GCTGCTCCCT 
CCTGCCGCCC CCTTCTGGCT CCTCAATGTG CTCTTCCCCC CGCACACCAC GCCCAAGGCT 
GAGCTCAGTA ACCACACACG GCCCGTCATC CTCGTGCCCG GCTGCCTGGG GAATCAGCTA 
GAAGCCAAGC TGGACAAACC AGATGTGGTG AACTGGATGT GCTACCGCAA GACAGAGGAC 
TTCTTCACCA TCTGGCTGGA TCTCAACATG TTCCTACCCC TTGGGGTAGA CTGCTGGATC 
GATAACACCA GGGTTGTCTA CAACCGGAGC TCTGGGCTCG TGTCCAACGC CCCTGGTGTC 
CAGATCCGCG TCCCTGGCTT TGGCAAGACC TACTCTGTGG AGTACCTGGA CAGCAGCAAG 
CTGGCAGGGT ACCTGCACAC ACTGGTGCAG AACCTGGTCA ACAATGGCTA CGTGCGGGAC 
GAGACTGTGC GCGCCGCCCC CTATGACTGG CGGCTGGAGC CCGGCCAGCA GGAGGAGTAC 
TACCGCAAGC TCGCAGGGCT GGTGGAGGAG ATGCACGCTG CCTATGGGAA GCCTGTCTTC 
CTCATTGGCC ACAGCCTCGG CTGTCTACAC TTGCTCTATT TCCTGCTGCG CCAGCCCCAG 
GCCTGGAAGG ACCGCTTTAT TGATGGCTTC ATCTCTCTTG GGGCTCCCTG GGGTGGCTCC 
ATCAAGCCCA TGCTGGTCTT GGCCTCAGGT GACAACCAGG GCATCCCCAT CATGTCCAGC 
ATCAAGCTGA AAGAGGAGCA GCGCATAACC ACCACCTCCC CCTGGATGTT TCCCTCTCGC 
ATGGCGTGGC CTGAGGACCA CGTGTTCATT TCCACACCCA GCTTCAACTA CACAGGCCGT 
GACTTCCAAC GCTTCTTTGC AGACCTGCAC TTTGAGGAAG GCTGGTACAT GTGGCTGCAG 
TCACGTGACC TCCTGGCAGG ACTCCCAGCA CCTGGTGTGG AAGTATACTG TCTTTACGGC 
GTGGGCCTGC CCACGCCCCG CACCTACATC TACGACCACG GCTTCCCCTA CACGGACCCT 
GTGGGTGTGC TCTATGAGGA TGGTGATGAC ACGGTGGCGA CCCGCAGCAC CGAGCTCTGT 
GGCCTGTGGC AGGGCCGCCA GCCACAGCCT GTGCACCTGC TGCCCCTGCA CGGGATACAG 
CATCTCAACA TGGTCTTCAG CAACCTGACC CTGGAGCACA TCAATGCCAT CCTGCTGGGT 
GCCTACCGCC AGGGTCCCCC TGCATCCCCG ACTGCCAGCC CAGAGCCCCC GCCTCCTGAA 
TAA