RASEF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RASEF
Entrez Gene ID	158158
Full Name	RAS and EF-hand domain containing
Synonyms	RAB45
General protein information	Preferred Names RAS and EF-hand domain containing Names ras and EF-hand domain-containing protein ras-related protein Rab-45
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q21.32
Summary	This gene is a member of the Rab family of GTPases that are involved in regulation of membrane traffic. The encoded protein contains an N-terminal EF-hand domain, a coiled-coil motif and a C-terminal Rab domain. A potential role as tumor suppressor has been indicated for this gene. [provided by RefSeq, Nov 2012].
Disorder MIM:	611344

mRNA and Protein(s)

mRNA	Protein	Name
XM_005251731.4	XP_005251788.1	ras and EF-hand domain-containing protein isoform X2
XM_005251730.4	XP_005251787.1	ras and EF-hand domain-containing protein isoform X1
XM_005251730.3	XP_005251787.1	ras and EF-hand domain-containing protein isoform X1
XM_005251731.3	XP_005251788.1	ras and EF-hand domain-containing protein isoform X2
NM_152573.4	NP_689786.2	ras and EF-hand domain-containing protein
NM_152573.3	NP_689786.2	ras and EF-hand domain-containing protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RASEF	NP_001098832.1
Danio rerio (zebrafish)	rasef	XP_699335.4
Caenorhabditis elegans (roundworm)	tag-312	NP_508523.1
Pan troglodytes (chimpanzee)	RASEF	XP_520093.3
Homo sapiens (human)	RASEF	NP_689786.2
Canis lupus familiaris (dog)	RASEF	XP_541269.2
Mus musculus (house mouse)	Rasef	NP_001017427.1
Rattus norvegicus (Norway rat)	Rasef	NP_001264263.1
	RASEF	XP_001104352.1
Gallus gallus (chicken)	RASEF	XP_003643075.2
Xenopus tropicalis (tropical clawed frog)	MGC147600	NP_001072877.1

Gene Ontology
Bibliography

Related articles in PubMed

Near-genomewide RNAi screening for regulators of BRAF(V600E) -induced senescence identifies RASEF, a gene epigenetically silenced in melanoma.
Kaplon J, H?mig-H?lzel C, Gao L, Meissl K, Verdegaal EM, van der Burg SH, van Doorn R, Peeper DS
Pigment cell & melanoma research27(4)640-52(2014 Jul)

Distinct acyl protein transferases and thioesterases control surface expression of calcium-activated potassium channels.
Tian L, McClafferty H, Knaus HG, Ruth P, Shipston MJ
The Journal of biological chemistry287(18)14718-25(2012 Apr)

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
Comuzzie AG, Cole SA, Laston SL, Voruganti VS, Haack K, Gibbs RA, Butte NF
PloS one7(12)e51954(2012)

Small GTPase RAB45-mediated p38 activation in apoptosis of chronic myeloid leukemia progenitor cells.
Nakamura S, Takemura T, Tan L, Nagata Y, Yokota D, Hirano I, Shigeno K, Shibata K, Fujie M, Fujisawa S, Ohnishi K
Carcinogenesis32(12)1758-72(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Near-genomewide RNAi screening for regulators of BRAF(V600E) -induced senescence identifies RASEF, a gene epigenetically silenced in melanoma.
Title: Near-genomewide RNAi screening for regulators of BRAF(V600E) -induced senescence identifies RASEF, a gene epigenetically silenced in melanoma.

overexpression of RAB45 increased the phosphorylation levels of p38, resulting in the induction of apoptosis and inhibition of proliferation of CML progenitor cells
Title: Small GTPase RAB45-mediated p38 activation in apoptosis of chronic myeloid leukemia progenitor cells.

Homozygosity, in combination with methylation, appears to be the mechanism targeting RASEF in uveal melanoma, and allelic imbalance at this locus supports a TSG role for RASEF.
Title: Epigenetic regulation identifies RASEF as a tumor-suppressor gene in uveal melanoma.

The following RASEF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASEF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu43015	XM_005251731.4 Latest version!	Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu01873	NM_152573.4 Latest version!	Homo sapiens RAS and EF-hand domain containing (RASEF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu43014	XM_005251730.4 Latest version!	Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu43014	XM_005251730.3	Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu43015	XM_005251731.3	Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu01873	NM_152573.3	Homo sapiens RAS and EF-hand domain containing (RASEF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RASEF ( XM_005251731.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251788.1 Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu43015
Clone ID Related Accession (Same CDS sequence)	XM_005251731.4 , XM_005251731.3
Accession Version	XM_005251731.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ras and EF-hand domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005251731.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GTTTACAAAC GTGGAGGCCT CCAGAGCCAG GCAGAGAAGA GCAAGTTAGT 
ACCTTGTACC AAAACATCAA CCTTGTGGAG CCAAGATTAA TTCAGCCATA TGAACATGTT 
ATAAAGAACT TCATCCGTGA GATCAGACTT CAAAGCACAG AAATGGAAAA TTTGGCCATT 
GCGGTGAAGA GAGCCCAGGA CAAGGCAGCT ATGCAGTTGA GTGAGTTGGA AGAGGAAATG 
GATCAGAGGA TTCAGGCTGC AGAACATAAG ACACGGAAAG ACGAAAAACG CAAAGCTGAG 
GAAGCCCTCA GTGACCTCAG ACGTCAGTAT GAAACTGAAG TAGGAGATCT GCAGGTGACC 
ATTAAAAAGC TAAGAAAGCT CGAAGAACAA TCAAAACGCG TAAGTCAAAA GGAAGATGTG 
GCTGCATTGA AAAAACAAAT TTATGATTTA TCAATGGAAA ACCAGAAAGT TAAGAAAGAC 
CTTTTAGAAG CACAGACAAA CATAGCCTTT CTTCAGAGTG AGTTAGATGC TTTGAAAAGT 
GATTATGCTG ATCAGAGTCT GAATACTGAA AGGGATCTGG AAATAATCCG AGCATACACA 
GAAGATCGAA ATAGTCTTGA GAGGCAAATT GAAATACTCC AAACAGCTAA CCGGAAGCTA 
CATGACAGTA ATGATGGCCT TAGAAGTGCC CTTGAAAACA GTTATAGCAA GTTCAACAGA 
TCTTTGCATA TAAATAATAT CTCACCAGGG AATACAATTT CTAGAAGCAG TCCCAAATTC 
ATTGGTCATT CCCCTCAACC TCTAGGCTAT GACAGGTCAT CCCGCTCTTC CTATGTGGAT 
GAGGACTGTG ACTCCCTGGC CCTCTGTGAT CCTCTGCAGA GGACAAATTG TGAAGTTGAC 
AGCCTGCCTG AAAGCTGCTT CGACAGCGGC TTGTCTACCT TGAGAGATCC CAATGAGTAT 
GACTCAGAAG TGGAATACAA GCACCAGAGG GGATTTCAGA GGTCACACGG GGTGCAGGAG 
AGCTTTGGAG GTGATGCTTC AGACACAGAT GTTCCTGACA TAAGGGATGA AGAGACATTT 
GGTTTAGAAG ATGTGGCTTC CGTCTTAGAC TGGAAGCCCC AAGGGTCTGT TAGTGAAGGC 
AGCATTGTTA GTTCATCAAG AAAGCCCATC TCAGCACTCT CGCCCCAGAC AGACCTGGTA 
GATGACAACG CTAAATCTTT TAGCTCACAG AAGGCTTACA AGATTGTACT TGCTGGGGAC 
GCTGCAGTGG GGAAGTCTAG TTTCCTCATG AGACTTTGCA AGAATGAATT TCGAGAAAAT 
ATAAGCGCCA CCCTGGGAGT TGATTTCCAA ATGAAAACCC TCATTGTGGA TGGAGAACGA 
ACAGTTCTGC AGCTCTGGGA TACAGCTGGT CAGGAGAGAT TCAGAAGTAT TGCCAAGTCT 
TACTTCAGAA AGGCAGATGG TGTTTTGCTG CTGTATGATG TTACATGTGA GAAAAGCTTT 
CTTAACATAC GAGAATGGGT AGATATGATT GAGGATGCAG CCCATGAGAC TGTTCCCATT 
ATGCTGGTAG GAAACAAGGC TGACATTCGT GACACTGCTG CTACAGAGGG ACAAAAATGT 
GTCCCAGGGC ACTTTGGAGA GAAACTGGCC ATGACGTATG GGGCATTATT CTGTGAAACA 
AGTGCCAAAG ATGGTTCTAA CATAGTGGAG GCTGTTCTGC ACCTTGCTCG AGAAGTGAAA 
AAGAGAACTG ACAAGGATGA CAGCAGATCC ATTACCAATC TAACCGGGAC CAATTCCAAA 
AAGTCACCAC AGATGAAGAA TTGTTGCAAT GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251788.1
CDS	1135..2970
Translation	MEEGLQTWRPPEPGREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

Target ORF information:

RefSeq Version	XM_005251731.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251731.4

ORF Insert Sequence:

ATGGAGGAGG GTTTACAAAC GTGGAGGCCT CCAGAGCCAG GCAGAGAAGA GCAAGTTAGT 
ACCTTGTACC AAAACATCAA CCTTGTGGAG CCAAGATTAA TTCAGCCATA TGAACATGTT 
ATAAAGAACT TCATCCGTGA GATCAGACTT CAAAGCACAG AAATGGAAAA TTTGGCCATT 
GCGGTGAAGA GAGCCCAGGA CAAGGCAGCT ATGCAGTTGA GTGAGTTGGA AGAGGAAATG 
GATCAGAGGA TTCAGGCTGC AGAACATAAG ACACGGAAAG ACGAAAAACG CAAAGCTGAG 
GAAGCCCTCA GTGACCTCAG ACGTCAGTAT GAAACTGAAG TAGGAGATCT GCAGGTGACC 
ATTAAAAAGC TAAGAAAGCT CGAAGAACAA TCAAAACGCG TAAGTCAAAA GGAAGATGTG 
GCTGCATTGA AAAAACAAAT TTATGATTTA TCAATGGAAA ACCAGAAAGT TAAGAAAGAC 
CTTTTAGAAG CACAGACAAA CATAGCCTTT CTTCAGAGTG AGTTAGATGC TTTGAAAAGT 
GATTATGCTG ATCAGAGTCT GAATACTGAA AGGGATCTGG AAATAATCCG AGCATACACA 
GAAGATCGAA ATAGTCTTGA GAGGCAAATT GAAATACTCC AAACAGCTAA CCGGAAGCTA 
CATGACAGTA ATGATGGCCT TAGAAGTGCC CTTGAAAACA GTTATAGCAA GTTCAACAGA 
TCTTTGCATA TAAATAATAT CTCACCAGGG AATACAATTT CTAGAAGCAG TCCCAAATTC 
ATTGGTCATT CCCCTCAACC TCTAGGCTAT GACAGGTCAT CCCGCTCTTC CTATGTGGAT 
GAGGACTGTG ACTCCCTGGC CCTCTGTGAT CCTCTGCAGA GGACAAATTG TGAAGTTGAC 
AGCCTGCCTG AAAGCTGCTT CGACAGCGGC TTGTCTACCT TGAGAGATCC CAATGAGTAT 
GACTCAGAAG TGGAATACAA GCACCAGAGG GGATTTCAGA GGTCACACGG GGTGCAGGAG 
AGCTTTGGAG GTGATGCTTC AGACACAGAT GTTCCTGACA TAAGGGATGA AGAGACATTT 
GGTTTAGAAG ATGTGGCTTC CGTCTTAGAC TGGAAGCCCC AAGGGTCTGT TAGTGAAGGC 
AGCATTGTTA GTTCATCAAG AAAGCCCATC TCAGCACTCT CGCCCCAGAC AGACCTGGTA 
GATGACAACG CTAAATCTTT TAGCTCACAG AAGGCTTACA AGATTGTACT TGCTGGGGAC 
GCTGCAGTGG GGAAGTCTAG TTTCCTCATG AGACTTTGCA AGAATGAATT TCGAGAAAAT 
ATAAGCGCCA CCCTGGGAGT TGATTTCCAA ATGAAAACCC TCATTGTGGA TGGAGAACGA 
ACAGTTCTGC AGCTCTGGGA TACAGCTGGT CAGGAGAGAT TCAGAAGTAT TGCCAAGTCT 
TACTTCAGAA AGGCAGATGG TGTTTTGCTG CTGTATGATG TTACATGTGA GAAAAGCTTT 
CTTAACATAC GAGAATGGGT AGATATGATT GAGGATGCAG CCCATGAGAC TGTTCCCATT 
ATGCTGGTAG GAAACAAGGC TGACATTCGT GACACTGCTG CTACAGAGGG ACAAAAATGT 
GTCCCAGGGC ACTTTGGAGA GAAACTGGCC ATGACGTATG GGGCATTATT CTGTGAAACA 
AGTGCCAAAG ATGGTTCTAA CATAGTGGAG GCTGTTCTGC ACCTTGCTCG AGAAGTGAAA 
AAGAGAACTG ACAAGGATGA CAGCAGATCC ATTACCAATC TAACCGGGAC CAATTCCAAA 
AAGTCACCAC AGATGAAGAA TTGTTGCAAT GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASEF ( XM_005251731.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251788.1 Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu43015
Clone ID Related Accession (Same CDS sequence)	XM_005251731.4 , XM_005251731.3
Accession Version	XM_005251731.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ras and EF-hand domain-containing protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005251731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GTTTACAAAC GTGGAGGCCT CCAGAGCCAG GCAGAGAAGA GCAAGTTAGT 
ACCTTGTACC AAAACATCAA CCTTGTGGAG CCAAGATTAA TTCAGCCATA TGAACATGTT 
ATAAAGAACT TCATCCGTGA GATCAGACTT CAAAGCACAG AAATGGAAAA TTTGGCCATT 
GCGGTGAAGA GAGCCCAGGA CAAGGCAGCT ATGCAGTTGA GTGAGTTGGA AGAGGAAATG 
GATCAGAGGA TTCAGGCTGC AGAACATAAG ACACGGAAAG ACGAAAAACG CAAAGCTGAG 
GAAGCCCTCA GTGACCTCAG ACGTCAGTAT GAAACTGAAG TAGGAGATCT GCAGGTGACC 
ATTAAAAAGC TAAGAAAGCT CGAAGAACAA TCAAAACGCG TAAGTCAAAA GGAAGATGTG 
GCTGCATTGA AAAAACAAAT TTATGATTTA TCAATGGAAA ACCAGAAAGT TAAGAAAGAC 
CTTTTAGAAG CACAGACAAA CATAGCCTTT CTTCAGAGTG AGTTAGATGC TTTGAAAAGT 
GATTATGCTG ATCAGAGTCT GAATACTGAA AGGGATCTGG AAATAATCCG AGCATACACA 
GAAGATCGAA ATAGTCTTGA GAGGCAAATT GAAATACTCC AAACAGCTAA CCGGAAGCTA 
CATGACAGTA ATGATGGCCT TAGAAGTGCC CTTGAAAACA GTTATAGCAA GTTCAACAGA 
TCTTTGCATA TAAATAATAT CTCACCAGGG AATACAATTT CTAGAAGCAG TCCCAAATTC 
ATTGGTCATT CCCCTCAACC TCTAGGCTAT GACAGGTCAT CCCGCTCTTC CTATGTGGAT 
GAGGACTGTG ACTCCCTGGC CCTCTGTGAT CCTCTGCAGA GGACAAATTG TGAAGTTGAC 
AGCCTGCCTG AAAGCTGCTT CGACAGCGGC TTGTCTACCT TGAGAGATCC CAATGAGTAT 
GACTCAGAAG TGGAATACAA GCACCAGAGG GGATTTCAGA GGTCACACGG GGTGCAGGAG 
AGCTTTGGAG GTGATGCTTC AGACACAGAT GTTCCTGACA TAAGGGATGA AGAGACATTT 
GGTTTAGAAG ATGTGGCTTC CGTCTTAGAC TGGAAGCCCC AAGGGTCTGT TAGTGAAGGC 
AGCATTGTTA GTTCATCAAG AAAGCCCATC TCAGCACTCT CGCCCCAGAC AGACCTGGTA 
GATGACAACG CTAAATCTTT TAGCTCACAG AAGGCTTACA AGATTGTACT TGCTGGGGAC 
GCTGCAGTGG GGAAGTCTAG TTTCCTCATG AGACTTTGCA AGAATGAATT TCGAGAAAAT 
ATAAGCGCCA CCCTGGGAGT TGATTTCCAA ATGAAAACCC TCATTGTGGA TGGAGAACGA 
ACAGTTCTGC AGCTCTGGGA TACAGCTGGT CAGGAGAGAT TCAGAAGTAT TGCCAAGTCT 
TACTTCAGAA AGGCAGATGG TGTTTTGCTG CTGTATGATG TTACATGTGA GAAAAGCTTT 
CTTAACATAC GAGAATGGGT AGATATGATT GAGGATGCAG CCCATGAGAC TGTTCCCATT 
ATGCTGGTAG GAAACAAGGC TGACATTCGT GACACTGCTG CTACAGAGGG ACAAAAATGT 
GTCCCAGGGC ACTTTGGAGA GAAACTGGCC ATGACGTATG GGGCATTATT CTGTGAAACA 
AGTGCCAAAG ATGGTTCTAA CATAGTGGAG GCTGTTCTGC ACCTTGCTCG AGAAGTGAAA 
AAGAGAACTG ACAAGGATGA CAGCAGATCC ATTACCAATC TAACCGGGAC CAATTCCAAA 
AAGTCACCAC AGATGAAGAA TTGTTGCAAT GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251788.1
CDS	1140..2975
Translation	MEEGLQTWRPPEPGREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

Target ORF information:

RefSeq Version	XM_005251731.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251731.3

ORF Insert Sequence:

ATGGAGGAGG GTTTACAAAC GTGGAGGCCT CCAGAGCCAG GCAGAGAAGA GCAAGTTAGT 
ACCTTGTACC AAAACATCAA CCTTGTGGAG CCAAGATTAA TTCAGCCATA TGAACATGTT 
ATAAAGAACT TCATCCGTGA GATCAGACTT CAAAGCACAG AAATGGAAAA TTTGGCCATT 
GCGGTGAAGA GAGCCCAGGA CAAGGCAGCT ATGCAGTTGA GTGAGTTGGA AGAGGAAATG 
GATCAGAGGA TTCAGGCTGC AGAACATAAG ACACGGAAAG ACGAAAAACG CAAAGCTGAG 
GAAGCCCTCA GTGACCTCAG ACGTCAGTAT GAAACTGAAG TAGGAGATCT GCAGGTGACC 
ATTAAAAAGC TAAGAAAGCT CGAAGAACAA TCAAAACGCG TAAGTCAAAA GGAAGATGTG 
GCTGCATTGA AAAAACAAAT TTATGATTTA TCAATGGAAA ACCAGAAAGT TAAGAAAGAC 
CTTTTAGAAG CACAGACAAA CATAGCCTTT CTTCAGAGTG AGTTAGATGC TTTGAAAAGT 
GATTATGCTG ATCAGAGTCT GAATACTGAA AGGGATCTGG AAATAATCCG AGCATACACA 
GAAGATCGAA ATAGTCTTGA GAGGCAAATT GAAATACTCC AAACAGCTAA CCGGAAGCTA 
CATGACAGTA ATGATGGCCT TAGAAGTGCC CTTGAAAACA GTTATAGCAA GTTCAACAGA 
TCTTTGCATA TAAATAATAT CTCACCAGGG AATACAATTT CTAGAAGCAG TCCCAAATTC 
ATTGGTCATT CCCCTCAACC TCTAGGCTAT GACAGGTCAT CCCGCTCTTC CTATGTGGAT 
GAGGACTGTG ACTCCCTGGC CCTCTGTGAT CCTCTGCAGA GGACAAATTG TGAAGTTGAC 
AGCCTGCCTG AAAGCTGCTT CGACAGCGGC TTGTCTACCT TGAGAGATCC CAATGAGTAT 
GACTCAGAAG TGGAATACAA GCACCAGAGG GGATTTCAGA GGTCACACGG GGTGCAGGAG 
AGCTTTGGAG GTGATGCTTC AGACACAGAT GTTCCTGACA TAAGGGATGA AGAGACATTT 
GGTTTAGAAG ATGTGGCTTC CGTCTTAGAC TGGAAGCCCC AAGGGTCTGT TAGTGAAGGC 
AGCATTGTTA GTTCATCAAG AAAGCCCATC TCAGCACTCT CGCCCCAGAC AGACCTGGTA 
GATGACAACG CTAAATCTTT TAGCTCACAG AAGGCTTACA AGATTGTACT TGCTGGGGAC 
GCTGCAGTGG GGAAGTCTAG TTTCCTCATG AGACTTTGCA AGAATGAATT TCGAGAAAAT 
ATAAGCGCCA CCCTGGGAGT TGATTTCCAA ATGAAAACCC TCATTGTGGA TGGAGAACGA 
ACAGTTCTGC AGCTCTGGGA TACAGCTGGT CAGGAGAGAT TCAGAAGTAT TGCCAAGTCT 
TACTTCAGAA AGGCAGATGG TGTTTTGCTG CTGTATGATG TTACATGTGA GAAAAGCTTT 
CTTAACATAC GAGAATGGGT AGATATGATT GAGGATGCAG CCCATGAGAC TGTTCCCATT 
ATGCTGGTAG GAAACAAGGC TGACATTCGT GACACTGCTG CTACAGAGGG ACAAAAATGT 
GTCCCAGGGC ACTTTGGAGA GAAACTGGCC ATGACGTATG GGGCATTATT CTGTGAAACA 
AGTGCCAAAG ATGGTTCTAA CATAGTGGAG GCTGTTCTGC ACCTTGCTCG AGAAGTGAAA 
AAGAGAACTG ACAAGGATGA CAGCAGATCC ATTACCAATC TAACCGGGAC CAATTCCAAA 
AAGTCACCAC AGATGAAGAA TTGTTGCAAT GGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASEF ( NM_152573.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_689786.2 Homo sapiens RAS and EF-hand domain containing (RASEF), mRNA.

Price & Availability↑

CloneID	OHu01873
Clone ID Related Accession (Same CDS sequence)	NM_152573.4 , NM_152573.3
Accession Version	NM_152573.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ras and EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL499602.6, BC023566.1 and BM982321.1. On Nov 23, 2018 this sequence version replaced NM_152573.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC023566.1, AK290986.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376447.4/ ENSP00000365630.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGCGG ATGGGGACGG AGAGGAGCTG GCCCGGCTGC GCTCAGTCTT CGCCGCCTGC 
GACGCGAACC GCTCGGGGCG CCTGGAGCGC GAGGAGTTCC GGGCACTGTG CACGGAGCTG 
CGGGTGCGGC CGGCCGACGC CGAGGCAGTA TTCCAGCGGC TGGACGCCGA CCGTGACGGC 
GCCATCACCT TCCAGGAGTT CGCGCGTGGC TTCCTCGGGT CCCTCCGCGG GGGGCGGCGC 
CGGGACTGGG GTCCTCTGGA TCCCGCGCCC GCCGTGTCTG AGGCGGGGCC GGAGACACAC 
GACAGCGAGG AGGACGAAGG CGACGAGGAC GCGGCGGCGG CGCTGGCCAC CTCGTGCGGC 
CCGGCGAGTC CCGGCCGGGC TTGGCAGGAT TTCCAGGCGC GACTTGGGGA CGAAGCCAAG 
TTCATTCCCA GAGAAGAGCA AGTTAGTACC TTGTACCAAA ACATCAACCT TGTGGAGCCA 
AGATTAATTC AGCCATATGA ACATGTTATA AAGAACTTCA TCCGTGAGAT CAGACTTCAA 
AGCACAGAAA TGGAAAATTT GGCCATTGCG GTGAAGAGAG CCCAGGACAA GGCAGCTATG 
CAGTTGAGTG AGTTGGAAGA GGAAATGGAT CAGAGGATTC AGGCTGCAGA ACATAAGACA 
CGGAAAGACG AAAAACGCAA AGCTGAGGAA GCCCTCAGTG ACCTCAGACG TCAGTATGAA 
ACTGAAGTAG GAGATCTGCA GGTGACCATT AAAAAGCTAA GAAAGCTCGA AGAACAATCA 
AAACGCGTAA GTCAAAAGGA AGATGTGGCT GCATTGAAAA AACAAATTTA TGATTTATCA 
ATGGAAAACC AGAAAGTTAA GAAAGACCTT TTAGAAGCAC AGACAAACAT AGCCTTTCTT 
CAGAGTGAGT TAGATGCTTT GAAAAGTGAT TATGCTGATC AGAGTCTGAA TACTGAAAGG 
GATCTGGAAA TAATCCGAGC ATACACAGAA GATCGAAATA GTCTTGAGAG GCAAATTGAA 
ATACTCCAAA CAGCTAACCG GAAGCTACAT GACAGTAATG ATGGCCTTAG AAGTGCCCTT 
GAAAACAGTT ATAGCAAGTT CAACAGATCT TTGCATATAA ATAATATCTC ACCAGGGAAT 
ACAATTTCTA GAAGCAGTCC CAAATTCATT GGTCATTCCC CTCAACCTCT AGGCTATGAC 
AGGTCATCCC GCTCTTCCTA TGTGGATGAG GACTGTGACT CCCTGGCCCT CTGTGATCCT 
CTGCAGAGGA CAAATTGTGA AGTTGACAGC CTGCCTGAAA GCTGCTTCGA CAGCGGCTTG 
TCTACCTTGA GAGATCCCAA TGAGTATGAC TCAGAAGTGG AATACAAGCA CCAGAGGGGA 
TTTCAGAGGT CACACGGGGT GCAGGAGAGC TTTGGAGGTG ATGCTTCAGA CACAGATGTT 
CCTGACATAA GGGATGAAGA GACATTTGGT TTAGAAGATG TGGCTTCCGT CTTAGACTGG 
AAGCCCCAAG GGTCTGTTAG TGAAGGCAGC ATTGTTAGTT CATCAAGAAA GCCCATCTCA 
GCACTCTCGC CCCAGACAGA CCTGGTAGAT GACAACGCTA AATCTTTTAG CTCACAGAAG 
GCTTACAAGA TTGTACTTGC TGGGGACGCT GCAGTGGGGA AGTCTAGTTT CCTCATGAGA 
CTTTGCAAGA ATGAATTTCG AGAAAATATA AGCGCCACCC TGGGAGTTGA TTTCCAAATG 
AAAACCCTCA TTGTGGATGG AGAACGAACA GTTCTGCAGC TCTGGGATAC AGCTGGTCAG 
GAGAGATTCA GAAGTATTGC CAAGTCTTAC TTCAGAAAGG CAGATGGTGT TTTGCTGCTG 
TATGATGTTA CATGTGAGAA AAGCTTTCTT AACATACGAG AATGGGTAGA TATGATTGAG 
GATGCAGCCC ATGAGACTGT TCCCATTATG CTGGTAGGAA ACAAGGCTGA CATTCGTGAC 
ACTGCTGCTA CAGAGGGACA AAAATGTGTC CCAGGGCACT TTGGAGAGAA ACTGGCCATG 
ACGTATGGGG CATTATTCTG TGAAACAAGT GCCAAAGATG GTTCTAACAT AGTGGAGGCT 
GTTCTGCACC TTGCTCGAGA AGTGAAAAAG AGAACTGACA AGGATGACAG CAGATCCATT 
ACCAATCTAA CCGGGACCAA TTCCAAAAAG TCACCACAGA TGAAGAATTG TTGCAATGGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689786.2
CDS	276..2498
Translation	MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

Target ORF information:

RefSeq Version	NM_152573.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS and EF-hand domain containing (RASEF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152573.4

ORF Insert Sequence:

ATGGAGGCGG ATGGGGACGG AGAGGAGCTG GCCCGGCTGC GCTCAGTCTT CGCCGCCTGC 
GACGCGAACC GCTCGGGGCG CCTGGAGCGC GAGGAGTTCC GGGCACTGTG CACGGAGCTG 
CGGGTGCGGC CGGCCGACGC CGAGGCAGTA TTCCAGCGGC TGGACGCCGA CCGTGACGGC 
GCCATCACCT TCCAGGAGTT CGCGCGTGGC TTCCTCGGGT CCCTCCGCGG GGGGCGGCGC 
CGGGACTGGG GTCCTCTGGA TCCCGCGCCC GCCGTGTCTG AGGCGGGGCC GGAGACACAC 
GACAGCGAGG AGGACGAAGG CGACGAGGAC GCGGCGGCGG CGCTGGCCAC CTCGTGCGGC 
CCGGCGAGTC CCGGCCGGGC TTGGCAGGAT TTCCAGGCGC GACTTGGGGA CGAAGCCAAG 
TTCATTCCCA GAGAAGAGCA AGTTAGTACC TTGTACCAAA ACATCAACCT TGTGGAGCCA 
AGATTAATTC AGCCATATGA ACATGTTATA AAGAACTTCA TCCGTGAGAT CAGACTTCAA 
AGCACAGAAA TGGAAAATTT GGCCATTGCG GTGAAGAGAG CCCAGGACAA GGCAGCTATG 
CAGTTGAGTG AGTTGGAAGA GGAAATGGAT CAGAGGATTC AGGCTGCAGA ACATAAGACA 
CGGAAAGACG AAAAACGCAA AGCTGAGGAA GCCCTCAGTG ACCTCAGACG TCAGTATGAA 
ACTGAAGTAG GAGATCTGCA GGTGACCATT AAAAAGCTAA GAAAGCTCGA AGAACAATCA 
AAACGCGTAA GTCAAAAGGA AGATGTGGCT GCATTGAAAA AACAAATTTA TGATTTATCA 
ATGGAAAACC AGAAAGTTAA GAAAGACCTT TTAGAAGCAC AGACAAACAT AGCCTTTCTT 
CAGAGTGAGT TAGATGCTTT GAAAAGTGAT TATGCTGATC AGAGTCTGAA TACTGAAAGG 
GATCTGGAAA TAATCCGAGC ATACACAGAA GATCGAAATA GTCTTGAGAG GCAAATTGAA 
ATACTCCAAA CAGCTAACCG GAAGCTACAT GACAGTAATG ATGGCCTTAG AAGTGCCCTT 
GAAAACAGTT ATAGCAAGTT CAACAGATCT TTGCATATAA ATAATATCTC ACCAGGGAAT 
ACAATTTCTA GAAGCAGTCC CAAATTCATT GGTCATTCCC CTCAACCTCT AGGCTATGAC 
AGGTCATCCC GCTCTTCCTA TGTGGATGAG GACTGTGACT CCCTGGCCCT CTGTGATCCT 
CTGCAGAGGA CAAATTGTGA AGTTGACAGC CTGCCTGAAA GCTGCTTCGA CAGCGGCTTG 
TCTACCTTGA GAGATCCCAA TGAGTATGAC TCAGAAGTGG AATACAAGCA CCAGAGGGGA 
TTTCAGAGGT CACACGGGGT GCAGGAGAGC TTTGGAGGTG ATGCTTCAGA CACAGATGTT 
CCTGACATAA GGGATGAAGA GACATTTGGT TTAGAAGATG TGGCTTCCGT CTTAGACTGG 
AAGCCCCAAG GGTCTGTTAG TGAAGGCAGC ATTGTTAGTT CATCAAGAAA GCCCATCTCA 
GCACTCTCGC CCCAGACAGA CCTGGTAGAT GACAACGCTA AATCTTTTAG CTCACAGAAG 
GCTTACAAGA TTGTACTTGC TGGGGACGCT GCAGTGGGGA AGTCTAGTTT CCTCATGAGA 
CTTTGCAAGA ATGAATTTCG AGAAAATATA AGCGCCACCC TGGGAGTTGA TTTCCAAATG 
AAAACCCTCA TTGTGGATGG AGAACGAACA GTTCTGCAGC TCTGGGATAC AGCTGGTCAG 
GAGAGATTCA GAAGTATTGC CAAGTCTTAC TTCAGAAAGG CAGATGGTGT TTTGCTGCTG 
TATGATGTTA CATGTGAGAA AAGCTTTCTT AACATACGAG AATGGGTAGA TATGATTGAG 
GATGCAGCCC ATGAGACTGT TCCCATTATG CTGGTAGGAA ACAAGGCTGA CATTCGTGAC 
ACTGCTGCTA CAGAGGGACA AAAATGTGTC CCAGGGCACT TTGGAGAGAA ACTGGCCATG 
ACGTATGGGG CATTATTCTG TGAAACAAGT GCCAAAGATG GTTCTAACAT AGTGGAGGCT 
GTTCTGCACC TTGCTCGAGA AGTGAAAAAG AGAACTGACA AGGATGACAG CAGATCCATT 
ACCAATCTAA CCGGGACCAA TTCCAAAAAG TCACCACAGA TGAAGAATTG TTGCAATGGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASEF ( NM_152573.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_689786.2 Homo sapiens RAS and EF-hand domain containing (RASEF), mRNA.

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CloneID	OHu01873
Clone ID Related Accession (Same CDS sequence)	NM_152573.4 , NM_152573.3
Accession Version	NM_152573.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	ras and EF-hand domain-containing protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC023566.1, AL499602.6 and BM982321.1. On Dec 1, 2012 this sequence version replaced NM_152573.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC023566.1, AK290986.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGG ATGGGGACGG AGAGGAGCTG GCCCGGCTGC GCTCAGTCTT CGCCGCCTGC 
GACGCGAACC GCTCGGGGCG CCTGGAGCGC GAGGAGTTCC GGGCACTGTG CACGGAGCTG 
CGGGTGCGGC CGGCCGACGC CGAGGCAGTA TTCCAGCGGC TGGACGCCGA CCGTGACGGC 
GCCATCACCT TCCAGGAGTT CGCGCGTGGC TTCCTCGGGT CCCTCCGCGG GGGGCGGCGC 
CGGGACTGGG GTCCTCTGGA TCCCGCGCCC GCCGTGTCTG AGGCGGGGCC GGAGACACAC 
GACAGCGAGG AGGACGAAGG CGACGAGGAC GCGGCGGCGG CGCTGGCCAC CTCGTGCGGC 
CCGGCGAGTC CCGGCCGGGC TTGGCAGGAT TTCCAGGCGC GACTTGGGGA CGAAGCCAAG 
TTCATTCCCA GAGAAGAGCA AGTTAGTACC TTGTACCAAA ACATCAACCT TGTGGAGCCA 
AGATTAATTC AGCCATATGA ACATGTTATA AAGAACTTCA TCCGTGAGAT CAGACTTCAA 
AGCACAGAAA TGGAAAATTT GGCCATTGCG GTGAAGAGAG CCCAGGACAA GGCAGCTATG 
CAGTTGAGTG AGTTGGAAGA GGAAATGGAT CAGAGGATTC AGGCTGCAGA ACATAAGACA 
CGGAAAGACG AAAAACGCAA AGCTGAGGAA GCCCTCAGTG ACCTCAGACG TCAGTATGAA 
ACTGAAGTAG GAGATCTGCA GGTGACCATT AAAAAGCTAA GAAAGCTCGA AGAACAATCA 
AAACGCGTAA GTCAAAAGGA AGATGTGGCT GCATTGAAAA AACAAATTTA TGATTTATCA 
ATGGAAAACC AGAAAGTTAA GAAAGACCTT TTAGAAGCAC AGACAAACAT AGCCTTTCTT 
CAGAGTGAGT TAGATGCTTT GAAAAGTGAT TATGCTGATC AGAGTCTGAA TACTGAAAGG 
GATCTGGAAA TAATCCGAGC ATACACAGAA GATCGAAATA GTCTTGAGAG GCAAATTGAA 
ATACTCCAAA CAGCTAACCG GAAGCTACAT GACAGTAATG ATGGCCTTAG AAGTGCCCTT 
GAAAACAGTT ATAGCAAGTT CAACAGATCT TTGCATATAA ATAATATCTC ACCAGGGAAT 
ACAATTTCTA GAAGCAGTCC CAAATTCATT GGTCATTCCC CTCAACCTCT AGGCTATGAC 
AGGTCATCCC GCTCTTCCTA TGTGGATGAG GACTGTGACT CCCTGGCCCT CTGTGATCCT 
CTGCAGAGGA CAAATTGTGA AGTTGACAGC CTGCCTGAAA GCTGCTTCGA CAGCGGCTTG 
TCTACCTTGA GAGATCCCAA TGAGTATGAC TCAGAAGTGG AATACAAGCA CCAGAGGGGA 
TTTCAGAGGT CACACGGGGT GCAGGAGAGC TTTGGAGGTG ATGCTTCAGA CACAGATGTT 
CCTGACATAA GGGATGAAGA GACATTTGGT TTAGAAGATG TGGCTTCCGT CTTAGACTGG 
AAGCCCCAAG GGTCTGTTAG TGAAGGCAGC ATTGTTAGTT CATCAAGAAA GCCCATCTCA 
GCACTCTCGC CCCAGACAGA CCTGGTAGAT GACAACGCTA AATCTTTTAG CTCACAGAAG 
GCTTACAAGA TTGTACTTGC TGGGGACGCT GCAGTGGGGA AGTCTAGTTT CCTCATGAGA 
CTTTGCAAGA ATGAATTTCG AGAAAATATA AGCGCCACCC TGGGAGTTGA TTTCCAAATG 
AAAACCCTCA TTGTGGATGG AGAACGAACA GTTCTGCAGC TCTGGGATAC AGCTGGTCAG 
GAGAGATTCA GAAGTATTGC CAAGTCTTAC TTCAGAAAGG CAGATGGTGT TTTGCTGCTG 
TATGATGTTA CATGTGAGAA AAGCTTTCTT AACATACGAG AATGGGTAGA TATGATTGAG 
GATGCAGCCC ATGAGACTGT TCCCATTATG CTGGTAGGAA ACAAGGCTGA CATTCGTGAC 
ACTGCTGCTA CAGAGGGACA AAAATGTGTC CCAGGGCACT TTGGAGAGAA ACTGGCCATG 
ACGTATGGGG CATTATTCTG TGAAACAAGT GCCAAAGATG GTTCTAACAT AGTGGAGGCT 
GTTCTGCACC TTGCTCGAGA AGTGAAAAAG AGAACTGACA AGGATGACAG CAGATCCATT 
ACCAATCTAA CCGGGACCAA TTCCAAAAAG TCACCACAGA TGAAGAATTG TTGCAATGGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_689786.2
CDS	262..2484
Translation	MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERDLEIIRAYTEDRNSLERQIEILQTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

Target ORF information:

RefSeq Version	NM_152573.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS and EF-hand domain containing (RASEF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_152573.3

ORF Insert Sequence:

ATGGAGGCGG ATGGGGACGG AGAGGAGCTG GCCCGGCTGC GCTCAGTCTT CGCCGCCTGC 
GACGCGAACC GCTCGGGGCG CCTGGAGCGC GAGGAGTTCC GGGCACTGTG CACGGAGCTG 
CGGGTGCGGC CGGCCGACGC CGAGGCAGTA TTCCAGCGGC TGGACGCCGA CCGTGACGGC 
GCCATCACCT TCCAGGAGTT CGCGCGTGGC TTCCTCGGGT CCCTCCGCGG GGGGCGGCGC 
CGGGACTGGG GTCCTCTGGA TCCCGCGCCC GCCGTGTCTG AGGCGGGGCC GGAGACACAC 
GACAGCGAGG AGGACGAAGG CGACGAGGAC GCGGCGGCGG CGCTGGCCAC CTCGTGCGGC 
CCGGCGAGTC CCGGCCGGGC TTGGCAGGAT TTCCAGGCGC GACTTGGGGA CGAAGCCAAG 
TTCATTCCCA GAGAAGAGCA AGTTAGTACC TTGTACCAAA ACATCAACCT TGTGGAGCCA 
AGATTAATTC AGCCATATGA ACATGTTATA AAGAACTTCA TCCGTGAGAT CAGACTTCAA 
AGCACAGAAA TGGAAAATTT GGCCATTGCG GTGAAGAGAG CCCAGGACAA GGCAGCTATG 
CAGTTGAGTG AGTTGGAAGA GGAAATGGAT CAGAGGATTC AGGCTGCAGA ACATAAGACA 
CGGAAAGACG AAAAACGCAA AGCTGAGGAA GCCCTCAGTG ACCTCAGACG TCAGTATGAA 
ACTGAAGTAG GAGATCTGCA GGTGACCATT AAAAAGCTAA GAAAGCTCGA AGAACAATCA 
AAACGCGTAA GTCAAAAGGA AGATGTGGCT GCATTGAAAA AACAAATTTA TGATTTATCA 
ATGGAAAACC AGAAAGTTAA GAAAGACCTT TTAGAAGCAC AGACAAACAT AGCCTTTCTT 
CAGAGTGAGT TAGATGCTTT GAAAAGTGAT TATGCTGATC AGAGTCTGAA TACTGAAAGG 
GATCTGGAAA TAATCCGAGC ATACACAGAA GATCGAAATA GTCTTGAGAG GCAAATTGAA 
ATACTCCAAA CAGCTAACCG GAAGCTACAT GACAGTAATG ATGGCCTTAG AAGTGCCCTT 
GAAAACAGTT ATAGCAAGTT CAACAGATCT TTGCATATAA ATAATATCTC ACCAGGGAAT 
ACAATTTCTA GAAGCAGTCC CAAATTCATT GGTCATTCCC CTCAACCTCT AGGCTATGAC 
AGGTCATCCC GCTCTTCCTA TGTGGATGAG GACTGTGACT CCCTGGCCCT CTGTGATCCT 
CTGCAGAGGA CAAATTGTGA AGTTGACAGC CTGCCTGAAA GCTGCTTCGA CAGCGGCTTG 
TCTACCTTGA GAGATCCCAA TGAGTATGAC TCAGAAGTGG AATACAAGCA CCAGAGGGGA 
TTTCAGAGGT CACACGGGGT GCAGGAGAGC TTTGGAGGTG ATGCTTCAGA CACAGATGTT 
CCTGACATAA GGGATGAAGA GACATTTGGT TTAGAAGATG TGGCTTCCGT CTTAGACTGG 
AAGCCCCAAG GGTCTGTTAG TGAAGGCAGC ATTGTTAGTT CATCAAGAAA GCCCATCTCA 
GCACTCTCGC CCCAGACAGA CCTGGTAGAT GACAACGCTA AATCTTTTAG CTCACAGAAG 
GCTTACAAGA TTGTACTTGC TGGGGACGCT GCAGTGGGGA AGTCTAGTTT CCTCATGAGA 
CTTTGCAAGA ATGAATTTCG AGAAAATATA AGCGCCACCC TGGGAGTTGA TTTCCAAATG 
AAAACCCTCA TTGTGGATGG AGAACGAACA GTTCTGCAGC TCTGGGATAC AGCTGGTCAG 
GAGAGATTCA GAAGTATTGC CAAGTCTTAC TTCAGAAAGG CAGATGGTGT TTTGCTGCTG 
TATGATGTTA CATGTGAGAA AAGCTTTCTT AACATACGAG AATGGGTAGA TATGATTGAG 
GATGCAGCCC ATGAGACTGT TCCCATTATG CTGGTAGGAA ACAAGGCTGA CATTCGTGAC 
ACTGCTGCTA CAGAGGGACA AAAATGTGTC CCAGGGCACT TTGGAGAGAA ACTGGCCATG 
ACGTATGGGG CATTATTCTG TGAAACAAGT GCCAAAGATG GTTCTAACAT AGTGGAGGCT 
GTTCTGCACC TTGCTCGAGA AGTGAAAAAG AGAACTGACA AGGATGACAG CAGATCCATT 
ACCAATCTAA CCGGGACCAA TTCCAAAAAG TCACCACAGA TGAAGAATTG TTGCAATGGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASEF ( XM_005251730.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251787.1 Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X1, mRNA.

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CloneID	OHu43014
Clone ID Related Accession (Same CDS sequence)	XM_005251730.4 , XM_005251730.3
Accession Version	XM_005251730.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ras and EF-hand domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005251730.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG ATGGGGACGG AGAGGAGCTG GCCCGGCTGC GCTCAGTCTT CGCCGCCTGC 
GACGCGAACC GCTCGGGGCG CCTGGAGCGC GAGGAGTTCC GGGCACTGTG CACGGAGCTG 
CGGGTGCGGC CGGCCGACGC CGAGGCAGTA TTCCAGCGGC TGGACGCCGA CCGTGACGGC 
GCCATCACCT TCCAGGAGTT CGCGCGTGGC TTCCTCGGGT CCCTCCGCGG GGGGCGGCGC 
CGGGACTGGG GTCCTCTGGA TCCCGCGCCC GCCGTGTCTG AGGCGGGGCC GGAGACACAC 
GACAGCGAGG AGGACGAAGG CGACGAGGAC GCGGCGGCGG CGCTGGCCAC CTCGTGCGGC 
CCGGCGAGTC CCGGCCGGGC TTGGCAGGAT TTCCAGGCGC GACTTGGGGA CGAAGCCAAG 
TTCATTCCCA GAGAAGAGCA AGTTAGTACC TTGTACCAAA ACATCAACCT TGTGGAGCCA 
AGATTAATTC AGCCATATGA ACATGTTATA AAGAACTTCA TCCGTGAGAT CAGACTTCAA 
AGCACAGAAA TGGAAAATTT GGCCATTGCG GTGAAGAGAG CCCAGGACAA GGCAGCTATG 
CAGTTGAGTG AGTTGGAAGA GGAAATGGAT CAGAGGATTC AGGCTGCAGA ACATAAGACA 
CGGAAAGACG AAAAACGCAA AGCTGAGGAA GCCCTCAGTG ACCTCAGACG TCAGTATGAA 
ACTGAAGTAG GAGATCTGCA GGTGACCATT AAAAAGCTAA GAAAGCTCGA AGAACAATCA 
AAACGCGTAA GTCAAAAGGA AGATGTGGCT GCATTGAAAA AACAAATTTA TGATTTATCA 
ATGGAAAACC AGAAAGTTAA GAAAGACCTT TTAGAAGCAC AGACAAACAT AGCCTTTCTT 
CAGAGTGAGT TAGATGCTTT GAAAAGTGAT TATGCTGATC AGAGTCTGAA TACTGAAAGA 
ACAGCTAACC GGAAGCTACA TGACAGTAAT GATGGCCTTA GAAGTGCCCT TGAAAACAGT 
TATAGCAAGT TCAACAGATC TTTGCATATA AATAATATCT CACCAGGGAA TACAATTTCT 
AGAAGCAGTC CCAAATTCAT TGGTCATTCC CCTCAACCTC TAGGCTATGA CAGGTCATCC 
CGCTCTTCCT ATGTGGATGA GGACTGTGAC TCCCTGGCCC TCTGTGATCC TCTGCAGAGG 
ACAAATTGTG AAGTTGACAG CCTGCCTGAA AGCTGCTTCG ACAGCGGCTT GTCTACCTTG 
AGAGATCCCA ATGAGTATGA CTCAGAAGTG GAATACAAGC ACCAGAGGGG ATTTCAGAGG 
TCACACGGGG TGCAGGAGAG CTTTGGAGGT GATGCTTCAG ACACAGATGT TCCTGACATA 
AGGGATGAAG AGACATTTGG TTTAGAAGAT GTGGCTTCCG TCTTAGACTG GAAGCCCCAA 
GGGTCTGTTA GTGAAGGCAG CATTGTTAGT TCATCAAGAA AGCCCATCTC AGCACTCTCG 
CCCCAGACAG ACCTGGTAGA TGACAACGCT AAATCTTTTA GCTCACAGAA GGCTTACAAG 
ATTGTACTTG CTGGGGACGC TGCAGTGGGG AAGTCTAGTT TCCTCATGAG ACTTTGCAAG 
AATGAATTTC GAGAAAATAT AAGCGCCACC CTGGGAGTTG ATTTCCAAAT GAAAACCCTC 
ATTGTGGATG GAGAACGAAC AGTTCTGCAG CTCTGGGATA CAGCTGGTCA GGAGAGATTC 
AGAAGTATTG CCAAGTCTTA CTTCAGAAAG GCAGATGGTG TTTTGCTGCT GTATGATGTT 
ACATGTGAGA AAAGCTTTCT TAACATACGA GAATGGGTAG ATATGATTGA GGATGCAGCC 
CATGAGACTG TTCCCATTAT GCTGGTAGGA AACAAGGCTG ACATTCGTGA CACTGCTGCT 
ACAGAGGGAC AAAAATGTGT CCCAGGGCAC TTTGGAGAGA AACTGGCCAT GACGTATGGG 
GCATTATTCT GTGAAACAAG TGCCAAAGAT GGTTCTAACA TAGTGGAGGC TGTTCTGCAC 
CTTGCTCGAG AAGTGAAAAA GAGAACTGAC AAGGATGACA GCAGATCCAT TACCAATCTA 
ACCGGGACCA ATTCCAAAAA GTCACCACAG ATGAAGAATT GTTGCAATGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251787.1
CDS	306..2459
Translation	MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

Target ORF information:

RefSeq Version	XM_005251730.4
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251730.4

ORF Insert Sequence:

ATGGAGGCGG ATGGGGACGG AGAGGAGCTG GCCCGGCTGC GCTCAGTCTT CGCCGCCTGC 
GACGCGAACC GCTCGGGGCG CCTGGAGCGC GAGGAGTTCC GGGCACTGTG CACGGAGCTG 
CGGGTGCGGC CGGCCGACGC CGAGGCAGTA TTCCAGCGGC TGGACGCCGA CCGTGACGGC 
GCCATCACCT TCCAGGAGTT CGCGCGTGGC TTCCTCGGGT CCCTCCGCGG GGGGCGGCGC 
CGGGACTGGG GTCCTCTGGA TCCCGCGCCC GCCGTGTCTG AGGCGGGGCC GGAGACACAC 
GACAGCGAGG AGGACGAAGG CGACGAGGAC GCGGCGGCGG CGCTGGCCAC CTCGTGCGGC 
CCGGCGAGTC CCGGCCGGGC TTGGCAGGAT TTCCAGGCGC GACTTGGGGA CGAAGCCAAG 
TTCATTCCCA GAGAAGAGCA AGTTAGTACC TTGTACCAAA ACATCAACCT TGTGGAGCCA 
AGATTAATTC AGCCATATGA ACATGTTATA AAGAACTTCA TCCGTGAGAT CAGACTTCAA 
AGCACAGAAA TGGAAAATTT GGCCATTGCG GTGAAGAGAG CCCAGGACAA GGCAGCTATG 
CAGTTGAGTG AGTTGGAAGA GGAAATGGAT CAGAGGATTC AGGCTGCAGA ACATAAGACA 
CGGAAAGACG AAAAACGCAA AGCTGAGGAA GCCCTCAGTG ACCTCAGACG TCAGTATGAA 
ACTGAAGTAG GAGATCTGCA GGTGACCATT AAAAAGCTAA GAAAGCTCGA AGAACAATCA 
AAACGCGTAA GTCAAAAGGA AGATGTGGCT GCATTGAAAA AACAAATTTA TGATTTATCA 
ATGGAAAACC AGAAAGTTAA GAAAGACCTT TTAGAAGCAC AGACAAACAT AGCCTTTCTT 
CAGAGTGAGT TAGATGCTTT GAAAAGTGAT TATGCTGATC AGAGTCTGAA TACTGAAAGA 
ACAGCTAACC GGAAGCTACA TGACAGTAAT GATGGCCTTA GAAGTGCCCT TGAAAACAGT 
TATAGCAAGT TCAACAGATC TTTGCATATA AATAATATCT CACCAGGGAA TACAATTTCT 
AGAAGCAGTC CCAAATTCAT TGGTCATTCC CCTCAACCTC TAGGCTATGA CAGGTCATCC 
CGCTCTTCCT ATGTGGATGA GGACTGTGAC TCCCTGGCCC TCTGTGATCC TCTGCAGAGG 
ACAAATTGTG AAGTTGACAG CCTGCCTGAA AGCTGCTTCG ACAGCGGCTT GTCTACCTTG 
AGAGATCCCA ATGAGTATGA CTCAGAAGTG GAATACAAGC ACCAGAGGGG ATTTCAGAGG 
TCACACGGGG TGCAGGAGAG CTTTGGAGGT GATGCTTCAG ACACAGATGT TCCTGACATA 
AGGGATGAAG AGACATTTGG TTTAGAAGAT GTGGCTTCCG TCTTAGACTG GAAGCCCCAA 
GGGTCTGTTA GTGAAGGCAG CATTGTTAGT TCATCAAGAA AGCCCATCTC AGCACTCTCG 
CCCCAGACAG ACCTGGTAGA TGACAACGCT AAATCTTTTA GCTCACAGAA GGCTTACAAG 
ATTGTACTTG CTGGGGACGC TGCAGTGGGG AAGTCTAGTT TCCTCATGAG ACTTTGCAAG 
AATGAATTTC GAGAAAATAT AAGCGCCACC CTGGGAGTTG ATTTCCAAAT GAAAACCCTC 
ATTGTGGATG GAGAACGAAC AGTTCTGCAG CTCTGGGATA CAGCTGGTCA GGAGAGATTC 
AGAAGTATTG CCAAGTCTTA CTTCAGAAAG GCAGATGGTG TTTTGCTGCT GTATGATGTT 
ACATGTGAGA AAAGCTTTCT TAACATACGA GAATGGGTAG ATATGATTGA GGATGCAGCC 
CATGAGACTG TTCCCATTAT GCTGGTAGGA AACAAGGCTG ACATTCGTGA CACTGCTGCT 
ACAGAGGGAC AAAAATGTGT CCCAGGGCAC TTTGGAGAGA AACTGGCCAT GACGTATGGG 
GCATTATTCT GTGAAACAAG TGCCAAAGAT GGTTCTAACA TAGTGGAGGC TGTTCTGCAC 
CTTGCTCGAG AAGTGAAAAA GAGAACTGAC AAGGATGACA GCAGATCCAT TACCAATCTA 
ACCGGGACCA ATTCCAAAAA GTCACCACAG ATGAAGAATT GTTGCAATGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASEF ( XM_005251730.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005251787.1 Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X1, mRNA.

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CloneID	OHu43014
Clone ID Related Accession (Same CDS sequence)	XM_005251730.4 , XM_005251730.3
Accession Version	XM_005251730.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ras and EF-hand domain-containing protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005251730.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCGG ATGGGGACGG AGAGGAGCTG GCCCGGCTGC GCTCAGTCTT CGCCGCCTGC 
GACGCGAACC GCTCGGGGCG CCTGGAGCGC GAGGAGTTCC GGGCACTGTG CACGGAGCTG 
CGGGTGCGGC CGGCCGACGC CGAGGCAGTA TTCCAGCGGC TGGACGCCGA CCGTGACGGC 
GCCATCACCT TCCAGGAGTT CGCGCGTGGC TTCCTCGGGT CCCTCCGCGG GGGGCGGCGC 
CGGGACTGGG GTCCTCTGGA TCCCGCGCCC GCCGTGTCTG AGGCGGGGCC GGAGACACAC 
GACAGCGAGG AGGACGAAGG CGACGAGGAC GCGGCGGCGG CGCTGGCCAC CTCGTGCGGC 
CCGGCGAGTC CCGGCCGGGC TTGGCAGGAT TTCCAGGCGC GACTTGGGGA CGAAGCCAAG 
TTCATTCCCA GAGAAGAGCA AGTTAGTACC TTGTACCAAA ACATCAACCT TGTGGAGCCA 
AGATTAATTC AGCCATATGA ACATGTTATA AAGAACTTCA TCCGTGAGAT CAGACTTCAA 
AGCACAGAAA TGGAAAATTT GGCCATTGCG GTGAAGAGAG CCCAGGACAA GGCAGCTATG 
CAGTTGAGTG AGTTGGAAGA GGAAATGGAT CAGAGGATTC AGGCTGCAGA ACATAAGACA 
CGGAAAGACG AAAAACGCAA AGCTGAGGAA GCCCTCAGTG ACCTCAGACG TCAGTATGAA 
ACTGAAGTAG GAGATCTGCA GGTGACCATT AAAAAGCTAA GAAAGCTCGA AGAACAATCA 
AAACGCGTAA GTCAAAAGGA AGATGTGGCT GCATTGAAAA AACAAATTTA TGATTTATCA 
ATGGAAAACC AGAAAGTTAA GAAAGACCTT TTAGAAGCAC AGACAAACAT AGCCTTTCTT 
CAGAGTGAGT TAGATGCTTT GAAAAGTGAT TATGCTGATC AGAGTCTGAA TACTGAAAGA 
ACAGCTAACC GGAAGCTACA TGACAGTAAT GATGGCCTTA GAAGTGCCCT TGAAAACAGT 
TATAGCAAGT TCAACAGATC TTTGCATATA AATAATATCT CACCAGGGAA TACAATTTCT 
AGAAGCAGTC CCAAATTCAT TGGTCATTCC CCTCAACCTC TAGGCTATGA CAGGTCATCC 
CGCTCTTCCT ATGTGGATGA GGACTGTGAC TCCCTGGCCC TCTGTGATCC TCTGCAGAGG 
ACAAATTGTG AAGTTGACAG CCTGCCTGAA AGCTGCTTCG ACAGCGGCTT GTCTACCTTG 
AGAGATCCCA ATGAGTATGA CTCAGAAGTG GAATACAAGC ACCAGAGGGG ATTTCAGAGG 
TCACACGGGG TGCAGGAGAG CTTTGGAGGT GATGCTTCAG ACACAGATGT TCCTGACATA 
AGGGATGAAG AGACATTTGG TTTAGAAGAT GTGGCTTCCG TCTTAGACTG GAAGCCCCAA 
GGGTCTGTTA GTGAAGGCAG CATTGTTAGT TCATCAAGAA AGCCCATCTC AGCACTCTCG 
CCCCAGACAG ACCTGGTAGA TGACAACGCT AAATCTTTTA GCTCACAGAA GGCTTACAAG 
ATTGTACTTG CTGGGGACGC TGCAGTGGGG AAGTCTAGTT TCCTCATGAG ACTTTGCAAG 
AATGAATTTC GAGAAAATAT AAGCGCCACC CTGGGAGTTG ATTTCCAAAT GAAAACCCTC 
ATTGTGGATG GAGAACGAAC AGTTCTGCAG CTCTGGGATA CAGCTGGTCA GGAGAGATTC 
AGAAGTATTG CCAAGTCTTA CTTCAGAAAG GCAGATGGTG TTTTGCTGCT GTATGATGTT 
ACATGTGAGA AAAGCTTTCT TAACATACGA GAATGGGTAG ATATGATTGA GGATGCAGCC 
CATGAGACTG TTCCCATTAT GCTGGTAGGA AACAAGGCTG ACATTCGTGA CACTGCTGCT 
ACAGAGGGAC AAAAATGTGT CCCAGGGCAC TTTGGAGAGA AACTGGCCAT GACGTATGGG 
GCATTATTCT GTGAAACAAG TGCCAAAGAT GGTTCTAACA TAGTGGAGGC TGTTCTGCAC 
CTTGCTCGAG AAGTGAAAAA GAGAACTGAC AAGGATGACA GCAGATCCAT TACCAATCTA 
ACCGGGACCA ATTCCAAAAA GTCACCACAG ATGAAGAATT GTTGCAATGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005251787.1
CDS	311..2464
Translation	MEADGDGEELARLRSVFAACDANRSGRLEREEFRALCTELRVRPADAEAVFQRLDADRDGAITFQEFARGFLGSLRGGRRRDWGPLDPAPAVSEAGPETHDSEEDEGDEDAAAALATSCGPASPGRAWQDFQARLGDEAKFIPREEQVSTLYQNINLVEPRLIQPYEHVIKNFIREIRLQSTEMENLAIAVKRAQDKAAMQLSELEEEMDQRIQAAEHKTRKDEKRKAEEALSDLRRQYETEVGDLQVTIKKLRKLEEQSKRVSQKEDVAALKKQIYDLSMENQKVKKDLLEAQTNIAFLQSELDALKSDYADQSLNTERTANRKLHDSNDGLRSALENSYSKFNRSLHINNISPGNTISRSSPKFIGHSPQPLGYDRSSRSSYVDEDCDSLALCDPLQRTNCEVDSLPESCFDSGLSTLRDPNEYDSEVEYKHQRGFQRSHGVQESFGGDASDTDVPDIRDEETFGLEDVASVLDWKPQGSVSEGSIVSSSRKPISALSPQTDLVDDNAKSFSSQKAYKIVLAGDAAVGKSSFLMRLCKNEFRENISATLGVDFQMKTLIVDGERTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNIREWVDMIEDAAHETVPIMLVGNKADIRDTAATEGQKCVPGHFGEKLAMTYGALFCETSAKDGSNIVEAVLHLAREVKKRTDKDDSRSITNLTGTNSKKSPQMKNCCNG

Target ORF information:

RefSeq Version	XM_005251730.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RAS and EF-hand domain containing (RASEF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005251730.3

ORF Insert Sequence:

ATGGAGGCGG ATGGGGACGG AGAGGAGCTG GCCCGGCTGC GCTCAGTCTT CGCCGCCTGC 
GACGCGAACC GCTCGGGGCG CCTGGAGCGC GAGGAGTTCC GGGCACTGTG CACGGAGCTG 
CGGGTGCGGC CGGCCGACGC CGAGGCAGTA TTCCAGCGGC TGGACGCCGA CCGTGACGGC 
GCCATCACCT TCCAGGAGTT CGCGCGTGGC TTCCTCGGGT CCCTCCGCGG GGGGCGGCGC 
CGGGACTGGG GTCCTCTGGA TCCCGCGCCC GCCGTGTCTG AGGCGGGGCC GGAGACACAC 
GACAGCGAGG AGGACGAAGG CGACGAGGAC GCGGCGGCGG CGCTGGCCAC CTCGTGCGGC 
CCGGCGAGTC CCGGCCGGGC TTGGCAGGAT TTCCAGGCGC GACTTGGGGA CGAAGCCAAG 
TTCATTCCCA GAGAAGAGCA AGTTAGTACC TTGTACCAAA ACATCAACCT TGTGGAGCCA 
AGATTAATTC AGCCATATGA ACATGTTATA AAGAACTTCA TCCGTGAGAT CAGACTTCAA 
AGCACAGAAA TGGAAAATTT GGCCATTGCG GTGAAGAGAG CCCAGGACAA GGCAGCTATG 
CAGTTGAGTG AGTTGGAAGA GGAAATGGAT CAGAGGATTC AGGCTGCAGA ACATAAGACA 
CGGAAAGACG AAAAACGCAA AGCTGAGGAA GCCCTCAGTG ACCTCAGACG TCAGTATGAA 
ACTGAAGTAG GAGATCTGCA GGTGACCATT AAAAAGCTAA GAAAGCTCGA AGAACAATCA 
AAACGCGTAA GTCAAAAGGA AGATGTGGCT GCATTGAAAA AACAAATTTA TGATTTATCA 
ATGGAAAACC AGAAAGTTAA GAAAGACCTT TTAGAAGCAC AGACAAACAT AGCCTTTCTT 
CAGAGTGAGT TAGATGCTTT GAAAAGTGAT TATGCTGATC AGAGTCTGAA TACTGAAAGA 
ACAGCTAACC GGAAGCTACA TGACAGTAAT GATGGCCTTA GAAGTGCCCT TGAAAACAGT 
TATAGCAAGT TCAACAGATC TTTGCATATA AATAATATCT CACCAGGGAA TACAATTTCT 
AGAAGCAGTC CCAAATTCAT TGGTCATTCC CCTCAACCTC TAGGCTATGA CAGGTCATCC 
CGCTCTTCCT ATGTGGATGA GGACTGTGAC TCCCTGGCCC TCTGTGATCC TCTGCAGAGG 
ACAAATTGTG AAGTTGACAG CCTGCCTGAA AGCTGCTTCG ACAGCGGCTT GTCTACCTTG 
AGAGATCCCA ATGAGTATGA CTCAGAAGTG GAATACAAGC ACCAGAGGGG ATTTCAGAGG 
TCACACGGGG TGCAGGAGAG CTTTGGAGGT GATGCTTCAG ACACAGATGT TCCTGACATA 
AGGGATGAAG AGACATTTGG TTTAGAAGAT GTGGCTTCCG TCTTAGACTG GAAGCCCCAA 
GGGTCTGTTA GTGAAGGCAG CATTGTTAGT TCATCAAGAA AGCCCATCTC AGCACTCTCG 
CCCCAGACAG ACCTGGTAGA TGACAACGCT AAATCTTTTA GCTCACAGAA GGCTTACAAG 
ATTGTACTTG CTGGGGACGC TGCAGTGGGG AAGTCTAGTT TCCTCATGAG ACTTTGCAAG 
AATGAATTTC GAGAAAATAT AAGCGCCACC CTGGGAGTTG ATTTCCAAAT GAAAACCCTC 
ATTGTGGATG GAGAACGAAC AGTTCTGCAG CTCTGGGATA CAGCTGGTCA GGAGAGATTC 
AGAAGTATTG CCAAGTCTTA CTTCAGAAAG GCAGATGGTG TTTTGCTGCT GTATGATGTT 
ACATGTGAGA AAAGCTTTCT TAACATACGA GAATGGGTAG ATATGATTGA GGATGCAGCC 
CATGAGACTG TTCCCATTAT GCTGGTAGGA AACAAGGCTG ACATTCGTGA CACTGCTGCT 
ACAGAGGGAC AAAAATGTGT CCCAGGGCAC TTTGGAGAGA AACTGGCCAT GACGTATGGG 
GCATTATTCT GTGAAACAAG TGCCAAAGAT GGTTCTAACA TAGTGGAGGC TGTTCTGCAC 
CTTGCTCGAG AAGTGAAAAA GAGAACTGAC AAGGATGACA GCAGATCCAT TACCAATCTA 
ACCGGGACCA ATTCCAAAAA GTCACCACAG ATGAAGAATT GTTGCAATGG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Near-genomewide RNAi screening for regulators of BRAF(V600E) -induced senescence identifies RASEF, a gene epigenetically silenced in melanoma.
Pigment cell & melanoma research27(4)640-52(2014 Jul)
Kaplon J,H?mig-H?lzel C,Gao L,Meissl K,Verdegaal EM,van der Burg SH,van Doorn R,Peeper DS

Distinct acyl protein transferases and thioesterases control surface expression of calcium-activated potassium channels.
The Journal of biological chemistry287(18)14718-25(2012 Apr)
Tian L,McClafferty H,Knaus HG,Ruth P,Shipston MJ

Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population.
PloS one7(12)e51954(2012)
Comuzzie AG,Cole SA,Laston SL,Voruganti VS,Haack K,Gibbs RA,Butte NF

Small GTPase RAB45-mediated p38 activation in apoptosis of chronic myeloid leukemia progenitor cells.
Carcinogenesis32(12)1758-72(2011 Dec)
Nakamura S,Takemura T,Tan L,Nagata Y,Yokota D,Hirano I,Shigeno K,Shibata K,Fujie M,Fujisawa S,Ohnishi K

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RASEF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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