Rasef cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rasef
Entrez Gene ID	298138
Full Name	RAS and EF hand domain containing
General protein information	Preferred Names RAS and EF hand domain containing Names ras and EF-hand domain-containing protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q31

mRNA and Protein(s)

mRNA	Protein	Name
NM_001277334.1	NP_001264263.1	ras and EF-hand domain-containing protein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RASEF	NP_001098832.1
Danio rerio (zebrafish)	rasef	XP_699335.4
Caenorhabditis elegans (roundworm)	tag-312	NP_508523.1
Pan troglodytes (chimpanzee)	RASEF	XP_520093.3
Homo sapiens (human)	RASEF	NP_689786.2
Canis lupus familiaris (dog)	RASEF	XP_541269.2
Mus musculus (house mouse)	Rasef	NP_001017427.1
Rattus norvegicus (Norway rat)	Rasef	NP_001264263.1
	RASEF	XP_001104352.1
Gallus gallus (chicken)	RASEF	XP_003643075.2
Xenopus tropicalis (tropical clawed frog)	MGC147600	NP_001072877.1

Gene Ontology
Bibliography

Related articles in PubMed

Distinct acyl protein transferases and thioesterases control surface expression of calcium-activated potassium channels.
Tian L, McClafferty H, Knaus HG, Ruth P, Shipston MJ
The Journal of biological chemistry287(18)14718-25(2012 Apr)

Characterization of Rab45/RASEF containing EF-hand domain and a coiled-coil motif as a self-associating GTPase.
Shintani M, Tada M, Kobayashi T, Kajiho H, Kontani K, Katada T
Biochemical and biophysical research communications357(3)661-7(2007 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Rasef gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rasef cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11864	NM_001277334.1 Latest version!	Rattus norvegicus RAS and EF hand domain containing (Rasef), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rasef ( NM_001277334.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001264263.1 Rattus norvegicus RAS and EF hand domain containing (Rasef), mRNA.

Price & Availability↑

CloneID	ORa11864
Clone ID Related Accession (Same CDS sequence)	NM_001277334.1
Accession Version	NM_001277334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Rattus norvegicus(Norway rat)
Product	ras and EF-hand domain-containing protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07048762.1. On or before Apr 4, 2013 this sequence version replaced XM_003754049.1, XM_003749964.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689599, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGCGG ACGCGGACCC CGAGGAACTT GCGCGGCTGC GCTCCGTCTT CACAACCTAT 
GACACGAACT GCTCCGGGCG CCTAGAACGT GCGGAGTTCC GCACGCTGTG CGCGGAGCTG 
CACGTGGCCG AAGCCGAAGC AGAGGCAGTG TTCCTGGGGC TCGACACGGA CCGCGATGGC 
GCAATCACCT TCCCGGAGTT CGCGCGTGGC TTCCGCGGAG CGCTCGGCCA GGACAGCGGA 
GAGGCCCAAG GCCTCTGGGA CGCGGTAGAG CCTGAGAGCC CCGGCCCCGC CTGGCGGGAC 
TTCCACACGC GCCTCGGAGA GGAGGCCCAG CTCATTCCCA GAAAAGAACA AGTTAGTACC 
CTGTATCAGA ACATTAACCT TGTGGAACCA AGACTAATCC AGCCATATGA ACATGTGATA 
AGGAACTTTC TCCGTGAGAT CAGACTCCAG AGCACAGAAA TGGAAAACTT GGCCATTGCA 
GTGAAAAGAG CCCAGGACAA GGCGGCCATT CAGCTAAGTG AGCTGGAAGA GGAAATGGAT 
CAGAGGATTC AAGCTGTGGA AAATGAGACC CGAAAAGATG AAAAACGCAA AGCTGAGGAA 
GCTCTCACTG ACCTCAGACG TCAGTATGAA ACAGAAGTAG GAGATTTACA AGTGACCATT 
AAAAGGTTAA AAAAGCTTGA AGAGCAGTCA AGAAAAATAA GTCAAAAGCA AGATGTGACT 
TCATTAAAGA AACAAATTTA TGATTTAACA ACGGAAAATC AAAAACTCAA AAAAGATCTT 
TTGGAGACAC AGACAAATTT AGCCTTTCTT CAGAGTGAAC TAGATGCTTT GAAAAGTGAT 
TATGCTGACC AGACTATGAA TTCTGAACGG GATCTGGAAC TAATCCGAGA GTTCAATGAA 
GATCGAAGTA ATCTTGAGAG GCAAATTGAA GTCCTCCAAA CAGCTAACCG GAAGCTTCAT 
GATAGTAATG ACGGCCTTAG AAGTGCCCTT GAAAACACTT ACAGCAAGCT CACCAAATCC 
TTGCGTATGA ATAACATATC ACCAGGGAAT ACAATTTCTA GCAGCAGTCC CAAGTTTAAT 
CATCATTCTT CTCAACCACT GGGCTACGAT AGGTCATCCC ATTCTTCATA TGCAGATGAG 
GATTGTGACT GTTTGGCTCT CTGTGACCCT CTGCAGAAGA TGAATTATGA AGTTGACAGC 
CTCCCCGAAA GCTGTTTTGA CAGTGGCCTG TCTACTCTGA GAGATTCTAA TGAGTGTGAT 
TCTGAGGTGG ACTACAAGCC CCAGGAAGGA TTTCAGAGTT TGCACAGAAC AGAGGAGAGC 
CTTGAGGGAG ATACGTCAGA CACAGATGTT CCTGATATGA GGGATGAAGA AACATTCGAT 
TGTGAAAGCG TGGCCTCTGT CTTACACGGG CATCCCCAGG GGTCTGCTGG TGAGGGCAGC 
ACTGTTAGTT CCTCCAGAAA GACCATCTCA GCTCTTTCAC CTGAAACAGA CATGGTGAAT 
AACACCTTGA AGTCCACATC TCAGAAGGCT TACAAGATTG TGCTGGCCGG TGATGCTGCA 
GTGGGAAAGT CCAGCTTCCT CATGCGACTC TGCAAGAATG AGTTTCAGGG AAACACAAGT 
GCAACCCTAG GAGTTGATTT CCAAATGAAA ACTCTGATGG TAGATGGTGA ACCAACAGTC 
CTTCAGCTCT GGGATACAGC TGGACAAGAA AGATTTAGAA GTATCGCCAA GTCTTACTTC 
CGAAAAGCAG ATGGTGTCTT ACTGCTGTAC GATGTTACTT GTGAGAAAAG CTTTCTTAAT 
GTACGAGAAT GGGTAGCTAT GGTTGAGGAT GGAACCCACA GATCCGTTCC TATCATGTTA 
GTAGGAAACA AGGCTGATCT TCGTGATGCT GCTACTGCAG AGAATCAAAA GTGTATCTCA 
TCACATCTTG GAGAAAAACT GGCCATGACC TATGGGGCAT TATTCTGTGA AACAAGCGCG 
AAAGATGGCT CCAATGTGGT GGAAGCTGTT CTCCATCTTG CTCGGGAAGT AAAGAAAAGG 
GCAGAGAATG ATGACAGCAA ATCCATCACC AGTCTGGCCA CTTCTACTTC CAGAAAGTCA 
CTGCAGATGA AGAACTGTTG CAATGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264263.1
CDS	1..2130
Translation	MEADADPEELARLRSVFTTYDTNCSGRLERAEFRTLCAELHVAEAEAEAVFLGLDTDRDGAITFPEFARGFRGALGQDSGEAQGLWDAVEPESPGPAWRDFHTRLGEEAQLIPRKEQVSTLYQNINLVEPRLIQPYEHVIRNFLREIRLQSTEMENLAIAVKRAQDKAAIQLSELEEEMDQRIQAVENETRKDEKRKAEEALTDLRRQYETEVGDLQVTIKRLKKLEEQSRKISQKQDVTSLKKQIYDLTTENQKLKKDLLETQTNLAFLQSELDALKSDYADQTMNSERDLELIREFNEDRSNLERQIEVLQTANRKLHDSNDGLRSALENTYSKLTKSLRMNNISPGNTISSSSPKFNHHSSQPLGYDRSSHSSYADEDCDCLALCDPLQKMNYEVDSLPESCFDSGLSTLRDSNECDSEVDYKPQEGFQSLHRTEESLEGDTSDTDVPDMRDEETFDCESVASVLHGHPQGSAGEGSTVSSSRKTISALSPETDMVNNTLKSTSQKAYKIVLAGDAAVGKSSFLMRLCKNEFQGNTSATLGVDFQMKTLMVDGEPTVLQLWDTAGQERFRSIAKSYFRKADGVLLLYDVTCEKSFLNVREWVAMVEDGTHRSVPIMLVGNKADLRDAATAENQKCISSHLGEKLAMTYGALFCETSAKDGSNVVEAVLHLAREVKKRAENDDSKSITSLATSTSRKSLQMKNCCNG

Target ORF information:

RefSeq Version	NM_001277334.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RAS and EF hand domain containing (Rasef), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277334.1

ORF Insert Sequence:

ATGGAGGCGG ACGCGGACCC CGAGGAACTT GCGCGGCTGC GCTCCGTCTT CACAACCTAT 
GACACGAACT GCTCCGGGCG CCTAGAACGT GCGGAGTTCC GCACGCTGTG CGCGGAGCTG 
CACGTGGCCG AAGCCGAAGC AGAGGCAGTG TTCCTGGGGC TCGACACGGA CCGCGATGGC 
GCAATCACCT TCCCGGAGTT CGCGCGTGGC TTCCGCGGAG CGCTCGGCCA GGACAGCGGA 
GAGGCCCAAG GCCTCTGGGA CGCGGTAGAG CCTGAGAGCC CCGGCCCCGC CTGGCGGGAC 
TTCCACACGC GCCTCGGAGA GGAGGCCCAG CTCATTCCCA GAAAAGAACA AGTTAGTACC 
CTGTATCAGA ACATTAACCT TGTGGAACCA AGACTAATCC AGCCATATGA ACATGTGATA 
AGGAACTTTC TCCGTGAGAT CAGACTCCAG AGCACAGAAA TGGAAAACTT GGCCATTGCA 
GTGAAAAGAG CCCAGGACAA GGCGGCCATT CAGCTAAGTG AGCTGGAAGA GGAAATGGAT 
CAGAGGATTC AAGCTGTGGA AAATGAGACC CGAAAAGATG AAAAACGCAA AGCTGAGGAA 
GCTCTCACTG ACCTCAGACG TCAGTATGAA ACAGAAGTAG GAGATTTACA AGTGACCATT 
AAAAGGTTAA AAAAGCTTGA AGAGCAGTCA AGAAAAATAA GTCAAAAGCA AGATGTGACT 
TCATTAAAGA AACAAATTTA TGATTTAACA ACGGAAAATC AAAAACTCAA AAAAGATCTT 
TTGGAGACAC AGACAAATTT AGCCTTTCTT CAGAGTGAAC TAGATGCTTT GAAAAGTGAT 
TATGCTGACC AGACTATGAA TTCTGAACGG GATCTGGAAC TAATCCGAGA GTTCAATGAA 
GATCGAAGTA ATCTTGAGAG GCAAATTGAA GTCCTCCAAA CAGCTAACCG GAAGCTTCAT 
GATAGTAATG ACGGCCTTAG AAGTGCCCTT GAAAACACTT ACAGCAAGCT CACCAAATCC 
TTGCGTATGA ATAACATATC ACCAGGGAAT ACAATTTCTA GCAGCAGTCC CAAGTTTAAT 
CATCATTCTT CTCAACCACT GGGCTACGAT AGGTCATCCC ATTCTTCATA TGCAGATGAG 
GATTGTGACT GTTTGGCTCT CTGTGACCCT CTGCAGAAGA TGAATTATGA AGTTGACAGC 
CTCCCCGAAA GCTGTTTTGA CAGTGGCCTG TCTACTCTGA GAGATTCTAA TGAGTGTGAT 
TCTGAGGTGG ACTACAAGCC CCAGGAAGGA TTTCAGAGTT TGCACAGAAC AGAGGAGAGC 
CTTGAGGGAG ATACGTCAGA CACAGATGTT CCTGATATGA GGGATGAAGA AACATTCGAT 
TGTGAAAGCG TGGCCTCTGT CTTACACGGG CATCCCCAGG GGTCTGCTGG TGAGGGCAGC 
ACTGTTAGTT CCTCCAGAAA GACCATCTCA GCTCTTTCAC CTGAAACAGA CATGGTGAAT 
AACACCTTGA AGTCCACATC TCAGAAGGCT TACAAGATTG TGCTGGCCGG TGATGCTGCA 
GTGGGAAAGT CCAGCTTCCT CATGCGACTC TGCAAGAATG AGTTTCAGGG AAACACAAGT 
GCAACCCTAG GAGTTGATTT CCAAATGAAA ACTCTGATGG TAGATGGTGA ACCAACAGTC 
CTTCAGCTCT GGGATACAGC TGGACAAGAA AGATTTAGAA GTATCGCCAA GTCTTACTTC 
CGAAAAGCAG ATGGTGTCTT ACTGCTGTAC GATGTTACTT GTGAGAAAAG CTTTCTTAAT 
GTACGAGAAT GGGTAGCTAT GGTTGAGGAT GGAACCCACA GATCCGTTCC TATCATGTTA 
GTAGGAAACA AGGCTGATCT TCGTGATGCT GCTACTGCAG AGAATCAAAA GTGTATCTCA 
TCACATCTTG GAGAAAAACT GGCCATGACC TATGGGGCAT TATTCTGTGA AACAAGCGCG 
AAAGATGGCT CCAATGTGGT GGAAGCTGTT CTCCATCTTG CTCGGGAAGT AAAGAAAAGG 
GCAGAGAATG ATGACAGCAA ATCCATCACC AGTCTGGCCA CTTCTACTTC CAGAAAGTCA 
CTGCAGATGA AGAACTGTTG CAATGGCTAA