CDX4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDX4
Entrez Gene ID	1046
Full Name	caudal type homeobox 4
General protein information	Preferred Names caudal type homeobox 4 Names homeobox protein CDX-4 caudal type homeobox transcription factor 4 caudal-type homeobox protein 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq13.2
Summary	This gene encodes a member of a small subfamily of homeobox containing transcription factors. The encoded protein may regulate homeobox gene expression during anteroposterior patterning and hematopoiesis. [provided by RefSeq, Aug 2012].
Disorder MIM:	300025

mRNA and Protein(s)

mRNA	Protein	Name
NM_005193.1	NP_005184.1	homeobox protein CDX-4

Pathways from BioSystems

Homologies

	CDX4	XP_001095271.1
Canis lupus familiaris (dog)	CDX4	XP_549080.1
Homo sapiens (human)	CDX4	NP_005184.1
Pan troglodytes (chimpanzee)	CDX4	XP_529040.3
Bos taurus (cattle)	CDX4	XP_003588202.1
Mus musculus (house mouse)	Cdx4	NP_031700.1
Rattus norvegicus (Norway rat)	Cdx4	NP_001100412.1

Gene Ontology
Bibliography

Related articles in PubMed

Human definitive hematopoietic specification from pluripotent stem cells is regulated by mesodermal expression of CDX4.
Creamer JP, Dege C, Ren Q, Ho JTK, Valentine MC, Druley TE, Sturgeon CM
Blood129(22)2988-2992(2017 06)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Corominas R, Yang X, Lin GN, Kang S, Shen Y, Ghamsari L, Broly M, Rodriguez M, Tam S, Trigg SA, Fan C, Yi S, Tasan M, Lemmens I, Kuang X, Zhao N, Malhotra D, Michaelson JJ, Vacic V, Calderwood MA, Roth FP, Tavernier J, Horvath S, Salehi-Ashtiani K, Korkin D, Sebat J, Hill DE, Hao T, Vidal M, Iakoucheva LM
Nature communications53650(2014 Apr)

?-Catenin activates the HOXA10 and CDX4 genes in myeloid progenitor cells.
Bei L, Shah C, Wang H, Huang W, Roy R, Eklund EA
The Journal of biological chemistry287(47)39589-601(2012 Nov)

Ectopic expression of CDX4 in human mesenchymal stem cells does not affect HOX gene expression or induce hematopoietic reprogramming.
Sz?ke K, Beckstr?m KJ, Brinchmann JE
Stem cell research9(2)135-42(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gene expression analyses on WNT-dependent KDR(+)CD235a(-) definitive hematopoietic mesoderm and WNT-independent KDR(+)CD235a(+) primitive hematopoietic mesoderm revealed strong CDX gene expression within definitive hematopoietic mesoderm. Temporal expression analyses revealed that CDX4 was expressed exclusively within definitive hematopoietic KDR(+)CD235a(-) mesoderm in a WNT- and fibroblast growth factor-dependent manner
Title: Human definitive hematopoietic specification from pluripotent stem cells is regulated by mesodermal expression of CDX4.

a molecular mechanisms through which increased expression of HoxA10 increases Cdx4 expression by direct CDX4 activation and by Fgf2-induced beta-catenin activity. This results in Cdx4-induced HoxA10-expression, creating a positive feedback mechanism
Title: ?-Catenin activates the HOXA10 and CDX4 genes in myeloid progenitor cells.

CDX4 gene was introduced to reprogram adipose tissue derived mesenchymal stem cells to differentiate along the hematopoietic lineage.
Title: Ectopic expression of CDX4 in human mesenchymal stem cells does not affect HOX gene expression or induce hematopoietic reprogramming.

positive feedback relationship between HoxA10 and Cdx4, which potentially amplified the contribution of either transcription factor to the pathogenesis of AML.
Title: HoxA10 activates CDX4 transcription and Cdx4 activates HOXA10 transcription in myeloid cells.

Expression of CDX1 and CDX4 is not correlated with the pathogenesis and relapse of adult ALL.
Title: [Expression and clinical significance of caudal type homeobox transcription factor-2 in adult acute lymphocytic leukemia].

The following CDX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu29895	NM_005193.1 Latest version!	Homo sapiens caudal type homeobox 4 (CDX4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDX4 ( NM_005193.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_005184.1 Homo sapiens caudal type homeobox 4 (CDX4), mRNA.

Price & Availability↑

CloneID	OHu29895
Clone ID Related Accession (Same CDS sequence)	NM_005193.1
Accession Version	NM_005193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 855bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-11
Organism	Homo sapiens(human)
Product	homeobox protein CDX-4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC128233.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC128233.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGTACGGAA GCTGTCTTTT GGAGAAAGAA GCAGGCATGT ACCCGGGCAC TCTCATGAGC 
CCTGGGGGCG ACGGCACAGC TGGGACAGGC GGCACAGGGG GCGGTGGGAG TCCGATGCCA 
GCCTCCAATT TCGCTGCGGC ACCGGCTTTC TCGCACTATA TGGGGTATCC TCATATGCCC 
AGCATGGATC CTCACTGGCC GTCTCTGGGA GTCTGGGGCT CACCCTACAG TCCCCCGCGA 
GAAGACTGGA GCGTGTATCC TGGGCCGTCT AGTACAATGG GCACAGTGCC GGTGAACGAC 
GTGACCTCTA GCCCCGCCGC TTTCTGCTCG ACCGACTACA GCAACTTGGG CCCTGTGGGC 
GGTGGAACTA GCGGCAGCAG CCTACCAGGC CAGGCTGGCG GGTCGCTTGT CCCGACGGAC 
GCAGGCGCCG CCAAGGCCAG TTCCCCCAGC AGGAGCCGCC ACAGCCCCTA TGCATGGATG 
CGCAAGACGG TGCAGGTGAC GGGGAAAACC AGGACAAAAG AAAAGTATCG TGTAGTTTAC 
ACTGATCATC AAAGATTGGA GCTGGAAAAG GAATTCCATT GCAATAGATA TATCACCATC 
CAGAGAAAAT CAGAGCTGGC AGTTAACCTG GGCCTTTCCG AGAGACAGGT GAAAATCTGG 
TTTCAGAATC GCAGAGCCAA GGAGAGAAAG ATGATCAAAA AGAAAATCTC CCAGTTTGAG 
AATAGTGGAG GCTCGGTGCA AAGTGACTCT GACTCCATCA GCCCTGGGGA ACTACCTAAC 
ACTTTTTTCA CCACACCATC TGCTGTTCGT GGATTTCAAC CTATTGAGAT ACAGCAGGTT 
ATAGTCTCCG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005184.1
CDS	1..855
Translation	MYGSCLLEKEAGMYPGTLMSPGGDGTAGTGGTGGGGSPMPASNFAAAPAFSHYMGYPHMPSMDPHWPSLGVWGSPYSPPREDWSVYPGPSSTMGTVPVNDVTSSPAAFCSTDYSNLGPVGGGTSGSSLPGQAGGSLVPTDAGAAKASSPSRSRHSPYAWMRKTVQVTGKTRTKEKYRVVYTDHQRLELEKEFHCNRYITIQRKSELAVNLGLSERQVKIWFQNRRAKERKMIKKKISQFENSGGSVQSDSDSISPGELPNTFFTTPSAVRGFQPIEIQQVIVSE

Target ORF information:

RefSeq Version	NM_005193.1
Organism	Homo sapiens(human)
Definition	Homo sapiens caudal type homeobox 4 (CDX4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005193.1

ORF Insert Sequence:

ATGTACGGAA GCTGTCTTTT GGAGAAAGAA GCAGGCATGT ACCCGGGCAC TCTCATGAGC 
CCTGGGGGCG ACGGCACAGC TGGGACAGGC GGCACAGGGG GCGGTGGGAG TCCGATGCCA 
GCCTCCAATT TCGCTGCGGC ACCGGCTTTC TCGCACTATA TGGGGTATCC TCATATGCCC 
AGCATGGATC CTCACTGGCC GTCTCTGGGA GTCTGGGGCT CACCCTACAG TCCCCCGCGA 
GAAGACTGGA GCGTGTATCC TGGGCCGTCT AGTACAATGG GCACAGTGCC GGTGAACGAC 
GTGACCTCTA GCCCCGCCGC TTTCTGCTCG ACCGACTACA GCAACTTGGG CCCTGTGGGC 
GGTGGAACTA GCGGCAGCAG CCTACCAGGC CAGGCTGGCG GGTCGCTTGT CCCGACGGAC 
GCAGGCGCCG CCAAGGCCAG TTCCCCCAGC AGGAGCCGCC ACAGCCCCTA TGCATGGATG 
CGCAAGACGG TGCAGGTGAC GGGGAAAACC AGGACAAAAG AAAAGTATCG TGTAGTTTAC 
ACTGATCATC AAAGATTGGA GCTGGAAAAG GAATTCCATT GCAATAGATA TATCACCATC 
CAGAGAAAAT CAGAGCTGGC AGTTAACCTG GGCCTTTCCG AGAGACAGGT GAAAATCTGG 
TTTCAGAATC GCAGAGCCAA GGAGAGAAAG ATGATCAAAA AGAAAATCTC CCAGTTTGAG 
AATAGTGGAG GCTCGGTGCA AAGTGACTCT GACTCCATCA GCCCTGGGGA ACTACCTAAC 
ACTTTTTTCA CCACACCATC TGCTGTTCGT GGATTTCAAC CTATTGAGAT ACAGCAGGTT 
ATAGTCTCCG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human definitive hematopoietic specification from pluripotent stem cells is regulated by mesodermal expression of CDX4.
Blood129(22)2988-2992(2017 06)
Creamer JP,Dege C,Ren Q,Ho JTK,Valentine MC,Druley TE,Sturgeon CM

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Protein interaction network of alternatively spliced isoforms from brain links genetic risk factors for autism.
Nature communications53650(2014 Apr)
Corominas R,Yang X,Lin GN,Kang S,Shen Y,Ghamsari L,Broly M,Rodriguez M,Tam S,Trigg SA,Fan C,Yi S,Tasan M,Lemmens I,Kuang X,Zhao N,Malhotra D,Michaelson JJ,Vacic V,Calderwood MA,Roth FP,Tavernier J,Horvath S,Salehi-Ashtiani K,Korkin D,Sebat J,Hill DE,Hao T,Vidal M,Iakoucheva LM

?-Catenin activates the HOXA10 and CDX4 genes in myeloid progenitor cells.
The Journal of biological chemistry287(47)39589-601(2012 Nov)
Bei L,Shah C,Wang H,Huang W,Roy R,Eklund EA

Ectopic expression of CDX4 in human mesenchymal stem cells does not affect HOX gene expression or induce hematopoietic reprogramming.
Stem cell research9(2)135-42(2012 Sep)
Sz?ke K,Beckstr?m KJ,Brinchmann JE