TOP3A cDNA ORF clone, Gavia stellata(red-throated loon)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOP3A
Entrez Gene ID	104255063
Full Name	DNA topoisomerase III alpha
Gene Type	protein-coding
Organism	Gavia stellata(red-throated loon)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009809005.1	XP_009807307.1	DNA topoisomerase 3-alpha

Pathways from BioSystems
Gene Ontology

The following TOP3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa136619	XM_009809005.1 Latest version!	Gavia stellata topoisomerase (DNA) III alpha (TOP3A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOP3A ( XM_009809005.1 ) cDNA ORF clone, Gavia stellata(red-throated loon) -> XP_009807307.1 Gavia stellata topoisomerase (DNA) III alpha (TOP3A), mRNA.

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CloneID	OGa136619
Clone ID Related Accession (Same CDS sequence)	XM_009809005.1
Accession Version	XM_009809005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-20
Organism	Gavia stellata(red-throated loon)
Product	DNA topoisomerase 3-alpha
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009294816.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gavia stellata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTGC AGAGGATCCG GAAGGTCCTG TGCGTGGCTG AGAAGAATGA TGCTGCCCGA 
GGGATTGCGG ATCTGCTCTC CAACAGCAGA ATGCGACGGA GAGAAGGGTT TTCCAAGTTC 
AACAAGATCT ACGAATACGA CTACCAGATG TTCGGCCAGA ATGTTACTAT GGTGATGACG 
TCCGTGTCGG GACACTTACT GGCTCATGAT TTTAAGCTGC CGTTTCGCAA ATGGCATAGC 
TGCAACCCTC TAGCTCTTTT TGATGCTGAA ATTGAGAAGT ATTGCCCTGA AAATTACATG 
GATATCAAGA GAACCCTTGA ACGAGAAGTC CAGCAGTGCC AAGCTTTGGT GATCTGGACT 
GACTGCGATC GAGAAGGAGA GAATATTGGC TTTGAGATAA TTCACGTTTG CAAAGCTGTA 
AAGCCAAACC TCCAGATTTT CCGAGCCCGT TTTTCTGAGA TTACGCTTCA TGCTGTCAGA 
ACAGCCTGCG AGAACCTTAC CCAACCAGAT CAAAAAACCA GTGATGCCGT TGATGTCAGG 
CAGGAGCTGG ACCTCAGGAT AGGTGCTGCC TTCACCAGAT TCCAGACGCT GAGGCTCCGG 
AAGATCTTCC CTGATATTTT AGCAGATCAG CTGATCAGCT ATGGTAGCTG CCAGTTCCCA 
ACGCTGGGGT TTGTAGTTGA ACGCTTTAAA GCCATCCAGG CCTTTGTTCC CGAAGCCTTC 
TATAAAATAA AAGTGACACA TGATCATGAA GATGGCAGTG TGGTCTTCAA CTGGAAGAGG 
AACCGGCTCT TTAATCACAC AGCGTGCCTT GTCCTTTACC AGATGTGTAT GGAGGATCCA 
GTAGCAACTG TTGTTGAGGT TGGGAGCAAG CCAAAGAGTA AATGGAGGCC CCTGCCCCTG 
GACACTGTGG AACTTGAGAA GCTGGCTTCC CGCAAACTGA AAATAAACGC AAAAGAAACC 
ATGAGAATAG CAGAAAAACT CTATACTCAA GGGTTCATCA GCTACCCCCG AACTGAGACC 
AACATTTTCC CCAAGGAGCT GAACCTCTCT GCCTTAGTAC AGCAGCAAAC ACAGGACCCC 
AACTGGGGAG CATTTGCACA GAGGATTTTG GATCAGGGTG GGCCAACCCC TCGGAGTGGA 
ACCAAATCAG ATCAGGCTCA CCCTCCCATT CACCCCACAA AATACACTGC CAGCCTGCAG 
GGCAATGAGC AGAGACTATA TGAATTCATC GTGCGCCATT TCTTGGCTTG CTGCTCTCAA 
GATGCCAAGG GACAGGAAAC AACTGTGGAG ATCGACATTG CTAATGAGCG ATTCATTGCT 
CAAGGACTAA TGATCCTGGC CCGAAATTAT CTGGAAGTCT ATCCTTATGA GAAGTGGAGT 
GATAAGGTTA TCCCACTGTA TCAGAAAGGG TCTCGCTTTC AGCCCACTAC AGTGGAGATG 
GTGGATGGGG AAACCAGCCC TCCATTGCTC CTCACAGAAG CGGATCTCAT TGCTCTCATG 
GAGAAACATG GCATTGGGAC TGACGCCACT CATGCTGAGC ACATTGAGAC AATTAAGACG 
CGGATGTATG TGGGTCTTAC AGCAGATCAG CGCTTCCTCC CAGGCCACCT GGGCATGGGG 
TTGGTTGAAG GCTATGATTC CATGGGCTAT GAGATGTCCA AGCCTGACCT TCGAGCTGAG 
CTGGAAGCTG ATCTGAAACT CATCTGTGAG GGGAAGAAAG ACAAATCTGC AGTGTTGCAG 
CAGCAGGTCC AAAAGTACAA GCAAGTCTTC ATCGAAGCTG TGGCCAGAGC CAACAAGTTG 
GACCAGGCCC TGGCTCAGTA TTTTGGAGAA GCCACAGAAA TTGCAGAGCA AGAGGACGTC 
TACCCAGCAA TGCCCGTTTC CGTTCGGAAG TGTCCACAGT GCAACAATGA TATGGTCCTG 
AAGACTAAGA AGAATGGCGG GTTCTATCTC AGCTGCACGG GCTATCCAGC ATGTAAAACT 
GCGGTCTGGT TTCCTGATTT TGTGCTGGAT GTGGCCAGGG ACGAGAGCGT CTGTGCTGTG 
TGTAAACCAC ATCCAGTTCA CAGACTGAAG TTTAAATTCA AGAGAGGCAG CGTTCCACCC 
ATGATGCCCC TGGAGTTTGT TGGTTGCATC GGAGGCTGTG ATGAGATGCT AAAAGAGCTC 
TTGGACCTGA AGTATTTACA CAGGTCATCC CAACCTGCAT CGTCAGCCAG CCAGCAAGCT 
AATCACTTGC AGGTCAACAA CTCCTTTAAT AGAGCTAGTG GTGAAAACAG GCACGTGAGG 
GGGACCACAA ACCTGGCACC AGGACATCTA CTCTCCTTGA GCTCAACGAG AACACCTAGG 
CCTGCTCCTT CAGCTGCTCC AGATGATGGG AATAACGCAG TGGTGTGCAA CTGCGGGAAC 
GAAGCCCTGC TGTTAACTGT GCGCAAAGAA GGGCCCAATC AAGGCAGGCA GTTTTACAAA 
TGCAGCGCCG GTACCTGCAA CTTTTTTCTC TGGGCTGATC AGCAGTCAGA CGACAGAAGC 
AACGCGACTC CACGGGGCTC TGCGCTACCC CAGCCCTTTG CAGGAAGGGG CCCAGCAGGA 
TTTCAGCGCT CAGGAGGAGG GAGAGGCCCA GAGCTCTTTG GAAGCAACAG CTCCAACTCA 
GGAGGCAGCA CAGTCTGCAA GTGCGACCAG CCAGCTGTCA CGCGCACCGT CCAAAAGGAT 
GGGCCCAACA AAGGGCGACA GTTCCACACC TGCTCTAAAC CCCGAGAGCA GCAGTGCGGC 
TTCTTCCAGT GGGCAGATGA AAACGTGGCA CCAGGGCCTT CTGGAGATGT CTCTTTGAAC 
CACTTTGGCA ACAGTGGAAA CTCAAGAGGG TTGGGAAGTA AAGCCAAGAG ACCAAGCAGT 
CTCTCCTCAG AAGCCACCGC CAAGAAACCA CGGACCTGTA GCATTTGCCA CCAGCCTGGC 
CACACTAGGA AAACCTGCCC TCAAACCCGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009807307.1
CDS	16..2988
Translation	MALQRIRKVLCVAEKNDAARGIADLLSNSRMRRREGFSKFNKIYEYDYQMFGQNVTMVMTSVSGHLLAHDFKLPFRKWHSCNPLALFDAEIEKYCPENYMDIKRTLEREVQQCQALVIWTDCDREGENIGFEIIHVCKAVKPNLQIFRARFSEITLHAVRTACENLTQPDQKTSDAVDVRQELDLRIGAAFTRFQTLRLRKIFPDILADQLISYGSCQFPTLGFVVERFKAIQAFVPEAFYKIKVTHDHEDGSVVFNWKRNRLFNHTACLVLYQMCMEDPVATVVEVGSKPKSKWRPLPLDTVELEKLASRKLKINAKETMRIAEKLYTQGFISYPRTETNIFPKELNLSALVQQQTQDPNWGAFAQRILDQGGPTPRSGTKSDQAHPPIHPTKYTASLQGNEQRLYEFIVRHFLACCSQDAKGQETTVEIDIANERFIAQGLMILARNYLEVYPYEKWSDKVIPLYQKGSRFQPTTVEMVDGETSPPLLLTEADLIALMEKHGIGTDATHAEHIETIKTRMYVGLTADQRFLPGHLGMGLVEGYDSMGYEMSKPDLRAELEADLKLICEGKKDKSAVLQQQVQKYKQVFIEAVARANKLDQALAQYFGEATEIAEQEDVYPAMPVSVRKCPQCNNDMVLKTKKNGGFYLSCTGYPACKTAVWFPDFVLDVARDESVCAVCKPHPVHRLKFKFKRGSVPPMMPLEFVGCIGGCDEMLKELLDLKYLHRSSQPASSASQQANHLQVNNSFNRASGENRHVRGTTNLAPGHLLSLSSTRTPRPAPSAAPDDGNNAVVCNCGNEALLLTVRKEGPNQGRQFYKCSAGTCNFFLWADQQSDDRSNATPRGSALPQPFAGRGPAGFQRSGGGRGPELFGSNSSNSGGSTVCKCDQPAVTRTVQKDGPNKGRQFHTCSKPREQQCGFFQWADENVAPGPSGDVSLNHFGNSGNSRGLGSKAKRPSSLSSEATAKKPRTCSICHQPGHTRKTCPQTR

Target ORF information:

RefSeq Version	XM_009809005.1
Organism	Gavia stellata(red-throated loon)
Definition	Gavia stellata topoisomerase (DNA) III alpha (TOP3A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009809005.1

ORF Insert Sequence:

ATGGCTTTGC AGAGGATCCG GAAGGTCCTG TGCGTGGCTG AGAAGAATGA TGCTGCCCGA 
GGGATTGCGG ATCTGCTCTC CAACAGCAGA ATGCGACGGA GAGAAGGGTT TTCCAAGTTC 
AACAAGATCT ACGAATACGA CTACCAGATG TTCGGCCAGA ATGTTACTAT GGTGATGACG 
TCCGTGTCGG GACACTTACT GGCTCATGAT TTTAAGCTGC CGTTTCGCAA ATGGCATAGC 
TGCAACCCTC TAGCTCTTTT TGATGCTGAA ATTGAGAAGT ATTGCCCTGA AAATTACATG 
GATATCAAGA GAACCCTTGA ACGAGAAGTC CAGCAGTGCC AAGCTTTGGT GATCTGGACT 
GACTGCGATC GAGAAGGAGA GAATATTGGC TTTGAGATAA TTCACGTTTG CAAAGCTGTA 
AAGCCAAACC TCCAGATTTT CCGAGCCCGT TTTTCTGAGA TTACGCTTCA TGCTGTCAGA 
ACAGCCTGCG AGAACCTTAC CCAACCAGAT CAAAAAACCA GTGATGCCGT TGATGTCAGG 
CAGGAGCTGG ACCTCAGGAT AGGTGCTGCC TTCACCAGAT TCCAGACGCT GAGGCTCCGG 
AAGATCTTCC CTGATATTTT AGCAGATCAG CTGATCAGCT ATGGTAGCTG CCAGTTCCCA 
ACGCTGGGGT TTGTAGTTGA ACGCTTTAAA GCCATCCAGG CCTTTGTTCC CGAAGCCTTC 
TATAAAATAA AAGTGACACA TGATCATGAA GATGGCAGTG TGGTCTTCAA CTGGAAGAGG 
AACCGGCTCT TTAATCACAC AGCGTGCCTT GTCCTTTACC AGATGTGTAT GGAGGATCCA 
GTAGCAACTG TTGTTGAGGT TGGGAGCAAG CCAAAGAGTA AATGGAGGCC CCTGCCCCTG 
GACACTGTGG AACTTGAGAA GCTGGCTTCC CGCAAACTGA AAATAAACGC AAAAGAAACC 
ATGAGAATAG CAGAAAAACT CTATACTCAA GGGTTCATCA GCTACCCCCG AACTGAGACC 
AACATTTTCC CCAAGGAGCT GAACCTCTCT GCCTTAGTAC AGCAGCAAAC ACAGGACCCC 
AACTGGGGAG CATTTGCACA GAGGATTTTG GATCAGGGTG GGCCAACCCC TCGGAGTGGA 
ACCAAATCAG ATCAGGCTCA CCCTCCCATT CACCCCACAA AATACACTGC CAGCCTGCAG 
GGCAATGAGC AGAGACTATA TGAATTCATC GTGCGCCATT TCTTGGCTTG CTGCTCTCAA 
GATGCCAAGG GACAGGAAAC AACTGTGGAG ATCGACATTG CTAATGAGCG ATTCATTGCT 
CAAGGACTAA TGATCCTGGC CCGAAATTAT CTGGAAGTCT ATCCTTATGA GAAGTGGAGT 
GATAAGGTTA TCCCACTGTA TCAGAAAGGG TCTCGCTTTC AGCCCACTAC AGTGGAGATG 
GTGGATGGGG AAACCAGCCC TCCATTGCTC CTCACAGAAG CGGATCTCAT TGCTCTCATG 
GAGAAACATG GCATTGGGAC TGACGCCACT CATGCTGAGC ACATTGAGAC AATTAAGACG 
CGGATGTATG TGGGTCTTAC AGCAGATCAG CGCTTCCTCC CAGGCCACCT GGGCATGGGG 
TTGGTTGAAG GCTATGATTC CATGGGCTAT GAGATGTCCA AGCCTGACCT TCGAGCTGAG 
CTGGAAGCTG ATCTGAAACT CATCTGTGAG GGGAAGAAAG ACAAATCTGC AGTGTTGCAG 
CAGCAGGTCC AAAAGTACAA GCAAGTCTTC ATCGAAGCTG TGGCCAGAGC CAACAAGTTG 
GACCAGGCCC TGGCTCAGTA TTTTGGAGAA GCCACAGAAA TTGCAGAGCA AGAGGACGTC 
TACCCAGCAA TGCCCGTTTC CGTTCGGAAG TGTCCACAGT GCAACAATGA TATGGTCCTG 
AAGACTAAGA AGAATGGCGG GTTCTATCTC AGCTGCACGG GCTATCCAGC ATGTAAAACT 
GCGGTCTGGT TTCCTGATTT TGTGCTGGAT GTGGCCAGGG ACGAGAGCGT CTGTGCTGTG 
TGTAAACCAC ATCCAGTTCA CAGACTGAAG TTTAAATTCA AGAGAGGCAG CGTTCCACCC 
ATGATGCCCC TGGAGTTTGT TGGTTGCATC GGAGGCTGTG ATGAGATGCT AAAAGAGCTC 
TTGGACCTGA AGTATTTACA CAGGTCATCC CAACCTGCAT CGTCAGCCAG CCAGCAAGCT 
AATCACTTGC AGGTCAACAA CTCCTTTAAT AGAGCTAGTG GTGAAAACAG GCACGTGAGG 
GGGACCACAA ACCTGGCACC AGGACATCTA CTCTCCTTGA GCTCAACGAG AACACCTAGG 
CCTGCTCCTT CAGCTGCTCC AGATGATGGG AATAACGCAG TGGTGTGCAA CTGCGGGAAC 
GAAGCCCTGC TGTTAACTGT GCGCAAAGAA GGGCCCAATC AAGGCAGGCA GTTTTACAAA 
TGCAGCGCCG GTACCTGCAA CTTTTTTCTC TGGGCTGATC AGCAGTCAGA CGACAGAAGC 
AACGCGACTC CACGGGGCTC TGCGCTACCC CAGCCCTTTG CAGGAAGGGG CCCAGCAGGA 
TTTCAGCGCT CAGGAGGAGG GAGAGGCCCA GAGCTCTTTG GAAGCAACAG CTCCAACTCA 
GGAGGCAGCA CAGTCTGCAA GTGCGACCAG CCAGCTGTCA CGCGCACCGT CCAAAAGGAT 
GGGCCCAACA AAGGGCGACA GTTCCACACC TGCTCTAAAC CCCGAGAGCA GCAGTGCGGC 
TTCTTCCAGT GGGCAGATGA AAACGTGGCA CCAGGGCCTT CTGGAGATGT CTCTTTGAAC 
CACTTTGGCA ACAGTGGAAA CTCAAGAGGG TTGGGAAGTA AAGCCAAGAG ACCAAGCAGT 
CTCTCCTCAG AAGCCACCGC CAAGAAACCA CGGACCTGTA GCATTTGCCA CCAGCCTGGC 
CACACTAGGA AAACCTGCCC TCAAACCCGC TGA