TRHDE cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRHDE
Entrez Gene ID	427868
Full Name	thyrotropin releasing hormone degrading enzyme
General protein information	Preferred Names thyrotropin releasing hormone degrading enzyme Names thyrotropin-releasing hormone-degrading ectoenzyme LOW QUALITY PROTEIN: thyrotropin-releasing hormone-degrading ectoenzyme
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015283004.1	XP_015138490.1	LOW QUALITY PROTEIN: thyrotropin-releasing hormone-degrading ectoenzyme
XM_015283004.2	XP_015138490.2	thyrotropin-releasing hormone-degrading ectoenzyme

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRHDE	NP_037513.1
Pan troglodytes (chimpanzee)	TRHDE	XP_522471.4
Canis lupus familiaris (dog)	TRHDE	XP_005625720.1
Xenopus tropicalis (tropical clawed frog)	trhde	XP_002939988.2
	TRHDE	XP_001109391.2
Bos taurus (cattle)	TRHDE	XP_002687216.3
Rattus norvegicus (Norway rat)	Trhde	NP_001102461.1
Mus musculus (house mouse)	Trhde	NP_666353.1
Gallus gallus (chicken)	TRHDE	XP_425442.3
Danio rerio (zebrafish)	LOC562409	XP_690889.2
Danio rerio (zebrafish)	si:ch211-149p5.1	XP_001345457.3

Gene Ontology

The following TRHDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRHDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGa142603	XM_015283004.2 Latest version!	Gallus gallus thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa29215	XM_015283004.1 Latest version!	Gallus gallus thyrotropin-releasing hormone degrading enzyme (TRHDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRHDE ( XM_015283004.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015138490.2 Gallus gallus thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.

Price & Availability↑

CloneID	OGa142603
Clone ID Related Accession (Same CDS sequence)	XM_015283004.2
Accession Version	XM_015283004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	thyrotropin-releasing hormone-degrading ectoenzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCTGG AGGAGAAGCG GGAGAAGCGG CCGCCCGTCA CCCCCATGAT GATCGAGGAG 
GAGGCAGCCC TGCGGAACGG CAGCCGGGGA CTGCCGCCGG GCCCCTGGGC CGAGGCGGCG 
GGGCCGCGAC CCCGCACGAC GGAGCGGCAC ATCGCCGTGC ACAAGCGCCT GGTGCTGGGC 
TTCGCCGTCT CCATCCTGGC GCTGCTGGCC GTGACCATGG TGGCCGTGCT GCTCAGCGTC 
CGCTTCGAGG AGTGCGGCGC GGCCGCCGAC GGACCCCCGC GGGCCGCCAA CAACGGGAGC 
CTGCCGGGAC CCGCGGCCGG GCAGCCCCGC GGCCGGGCCG AGCCGCGGGA GGAGGCGCGG 
GGCGGGGAGG AGGCGGCGGA GCGGCGGCAG GAGGAGGAGG AGGAGGAGGA GGAGGAGTGG 
CAGCGGCGGC GGCAGCTGCC GCCCTGGGCC CGGCCGCGGC TGCCGCGGCA CCTGCGGCCG 
CTGCACTACA ACCTGATGCT GAGCGCCTTC ATGGAGAACT TCACCTTCTG CGGGGAGGTG 
AACGTGCAGC TGGAGGTGCG CAACGCCAGC CGCTACATCG TGCTCCACGC CCACCGCATG 
CACATCGAGG CGGTGCGCGT GGCCGAGGAC AAGCTGGCGG GCGCCGTGCG GGTGGCACGG 
TCCTTCCTCT ACCCCCAGAC CCAGGTCCTG GTCGTCGTCC TCAACCGCAG CCTGGAGGTG 
CAGCGGAGTT ACAACCTCAA GATCATCTAC AACGCGCTCA TCGAGAACGA GCTGCTCGGC 
TTCTTCCGCA GCTCCTACGT CCTGCACGGC GAGCGCAGGT TTCTTGGTGT TACGCAGTTT 
TCTCCTACTC ATGCCAGAAA AGCCTTTCCT TGCTTTGATG AGCCCATCTA CAAGGCCACT 
TTCAAAATCA GCATCAAGCA TCAAGCAACT TATATATCTT TGTCCAACAT GCCAGTTGAA 
ACTTCTGTTT TTGAAGAAGA TGGATGGGTT ACAGATCACT TTTCACAAAC CCCTCTCATG 
TCCACATATT ACCTTGCTTG GGCAGTGTGC AATTTTACGT ACCGGGAGAC CATCACCAAG 
AGTGGAGTGG TTGTACGACT GTACGCAAGG CCTGATGCAA TCCGAAGAGG ATCAGGAGAC 
TATGCCCTAA ATATTACAAG AAGACTCATT GAATTTTATG AAGATTATTT TAAAGTGCCC 
TATTCCCTAC CAAAATTAGA TCTGTTAGCT GTGCCTAAAC ATCCCTATGC TGCTATGGAG 
AACTGGGGAC TGAGTGTTTT TGTGGAGCAA AGGATACTAC TGGATCCAAG CATTTCTTCT 
ATATCATACC TGTTGGATGT CACCATGGTC ATTGTACATG AGCTATGTCA TCAGTGGTTT 
GGTGACCTTG TGACTCCAGT TTGGTGGGAG GATGTGTGGT TGAAAGAGGG TTTTGCTCAC 
TATTTTGAAT TTATCGGGAC AGACTACCTC TACCCAGGAT GGAACATGGA AAAGCAGAGA 
TTTCTGACAG ATGTTCTACA TGAAGTGATG TTGCTGGATG GTTTGACCAG TTCGCATCCA 
GTATCCCAGG AGGTGCAGCA AGCCACAGAC ATTGACAGGG TGTTTGACTG GATTGCATAT 
AAAAAGGGTG CAGCTTTAAT TCGAATGTTG GCTAATTTTA TGGGTCACTC TGTGTTTCAA 
ATGGGCTTGC AAGACTATTT AACCATTCAC AAGTATGGCA ATGCAGCCAG AAACGATCTC 
TGGAACACAT TGTCAAAGGC TTTAAAAAGG GTTGGAAAAT CTGTAAATAT CCAAGAAGTA 
ATGGATCAGT GGACGCTACA GATGGGTTAT CCTGTTATCA CCATCTTGGG AAATGAAACT 
GCAGACAATG TCATAGTAAT CTCCCAGGAG CGTTTTGTTT ATGATAGCGA TACTAAAACC 
AAGGATTCTG GCCTTGGAGA CAACAGCTAC TTGTGGCAGA TTCCATTAAC AATTGCAGTA 
GGAAATACGA GCCACATCTC ATCAGAGGCA ATTATATGGG TGTCTAACAA ATCAGAACAC 
CACAGAATTC CTGCTTTGGA AGAGGCAAGT TGGTTGCTGG GAAATATCAA CCAGACTGGC 
TACTTCAGAG TTAATTATGA TATTAGGAAT TGGAGGCTGC TGATTAATCA GCTGACAAGG 
AACCACGAGG TTATCTCTGT CAGCGATCGA GCAGGCTTGA TCGATGATGC ATTCAATCTA 
GCCAGAGCTG GTTATTTGCC TCAGAATATT CCTTTGGAGA TTATCAGATA CCTTTCAGAG 
GAGAAGGATT TTCTTCCTTG GCATGCAGCC AGCCAAGCTC TTTATCCTCT AGATAAATTA 
CTGGACCGCA CAGAAAACTA CAACATCTTC AGTGAGTATA TTTTAAGACA AGTCGCATCA 
ATGTATCTCA AGCTTGGATG GCCAACGAAT AATTTAAACA AATCGCTTGT TCAAGCATCC 
TACCAACATG AAGAGTTGCG TCGGGAAGTC ATCATGTTGG CCTGCAGCTT TGGTAACAAA 
CACTGTCACC AGCAGGCTGC AACATTAATT TCAGATTGGA TTTCTAGCAA TAGAAACCGA 
ATCCCACTTA ATGTGAGAGA CATTGTCTAC TGTACAGGAG TTTCACTGAT GGATGAAGAT 
GTATGGGAGT TCATTTGGAT GAAATTTCAT TCTACATTAG CAGTGTCTGA GAAGAAAATA 
TTATTAGAAG CCTTAACTTG CAGTGATGAC AGAAACTTGC TTAACAGACT TTTGAATTTG 
TCTCTCAACT CAGAAGTTGT CCTGGATCAG GATGCAATTG ATGTGATAAT TCATGTAGCT 
CGAAATCCAC ATGGAAGAGA TCTTGCTTGG AAGTTTTTCA GAGAAAAATG GAAAATATTA 
AATGCCAGGT ATGGAGAAGC ATTATTTATG AATTCGAAGC TTATCAGTGG AGTCACAGAG 
TTCCTCAATA CCGAAGAAGA ACTAAGAGAG CTAAAGAACT TTATAAAGAG TTATGAGGAG 
GGAGCTGCTG CCTCTTTCTC TCGTGCTGTG GAAACTGTGG AAGCCAATGT GCGGTGGCAA 
AGACTTTATA AGGAAGAACT ATTCCAGTGG TTAAGAAAAT CCTTGACACA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015138490.2
CDS	5887..9000
Translation	MALEEKREKRPPVTPMMIEEEAALRNGSRGLPPGPWAEAAGPRPRTTERHIAVHKRLVLGFAVSILALLAVTMVAVLLSVRFEECGAAADGPPRAANNGSLPGPAAGQPRGRAEPREEARGGEEAAERRQEEEEEEEEEWQRRRQLPPWARPRLPRHLRPLHYNLMLSAFMENFTFCGEVNVQLEVRNASRYIVLHAHRMHIEAVRVAEDKLAGAVRVARSFLYPQTQVLVVVLNRSLEVQRSYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYISLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAVCNFTYRETITKSGVVVRLYARPDAIRRGSGDYALNITRRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSVFVEQRILLDPSISSISYLLDVTMVIVHELCHQWFGDLVTPVWWEDVWLKEGFAHYFEFIGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLTSSHPVSQEVQQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQMGLQDYLTIHKYGNAARNDLWNTLSKALKRVGKSVNIQEVMDQWTLQMGYPVITILGNETADNVIVISQERFVYDSDTKTKDSGLGDNSYLWQIPLTIAVGNTSHISSEAIIWVSNKSEHHRIPALEEASWLLGNINQTGYFRVNYDIRNWRLLINQLTRNHEVISVSDRAGLIDDAFNLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASQALYPLDKLLDRTENYNIFSEYILRQVASMYLKLGWPTNNLNKSLVQASYQHEELRREVIMLACSFGNKHCHQQAATLISDWISSNRNRIPLNVRDIVYCTGVSLMDEDVWEFIWMKFHSTLAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFREKWKILNARYGEALFMNSKLISGVTEFLNTEEELRELKNFIKSYEEGAAASFSRAVETVEANVRWQRLYKEELFQWLRKSLTH

Target ORF information:

RefSeq Version	XM_015283004.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyrotropin releasing hormone degrading enzyme (TRHDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283004.2

ORF Insert Sequence:

ATGGCCCTGG AGGAGAAGCG GGAGAAGCGG CCGCCCGTCA CCCCCATGAT GATCGAGGAG 
GAGGCAGCCC TGCGGAACGG CAGCCGGGGA CTGCCGCCGG GCCCCTGGGC CGAGGCGGCG 
GGGCCGCGAC CCCGCACGAC GGAGCGGCAC ATCGCCGTGC ACAAGCGCCT GGTGCTGGGC 
TTCGCCGTCT CCATCCTGGC GCTGCTGGCC GTGACCATGG TGGCCGTGCT GCTCAGCGTC 
CGCTTCGAGG AGTGCGGCGC GGCCGCCGAC GGACCCCCGC GGGCCGCCAA CAACGGGAGC 
CTGCCGGGAC CCGCGGCCGG GCAGCCCCGC GGCCGGGCCG AGCCGCGGGA GGAGGCGCGG 
GGCGGGGAGG AGGCGGCGGA GCGGCGGCAG GAGGAGGAGG AGGAGGAGGA GGAGGAGTGG 
CAGCGGCGGC GGCAGCTGCC GCCCTGGGCC CGGCCGCGGC TGCCGCGGCA CCTGCGGCCG 
CTGCACTACA ACCTGATGCT GAGCGCCTTC ATGGAGAACT TCACCTTCTG CGGGGAGGTG 
AACGTGCAGC TGGAGGTGCG CAACGCCAGC CGCTACATCG TGCTCCACGC CCACCGCATG 
CACATCGAGG CGGTGCGCGT GGCCGAGGAC AAGCTGGCGG GCGCCGTGCG GGTGGCACGG 
TCCTTCCTCT ACCCCCAGAC CCAGGTCCTG GTCGTCGTCC TCAACCGCAG CCTGGAGGTG 
CAGCGGAGTT ACAACCTCAA GATCATCTAC AACGCGCTCA TCGAGAACGA GCTGCTCGGC 
TTCTTCCGCA GCTCCTACGT CCTGCACGGC GAGCGCAGGT TTCTTGGTGT TACGCAGTTT 
TCTCCTACTC ATGCCAGAAA AGCCTTTCCT TGCTTTGATG AGCCCATCTA CAAGGCCACT 
TTCAAAATCA GCATCAAGCA TCAAGCAACT TATATATCTT TGTCCAACAT GCCAGTTGAA 
ACTTCTGTTT TTGAAGAAGA TGGATGGGTT ACAGATCACT TTTCACAAAC CCCTCTCATG 
TCCACATATT ACCTTGCTTG GGCAGTGTGC AATTTTACGT ACCGGGAGAC CATCACCAAG 
AGTGGAGTGG TTGTACGACT GTACGCAAGG CCTGATGCAA TCCGAAGAGG ATCAGGAGAC 
TATGCCCTAA ATATTACAAG AAGACTCATT GAATTTTATG AAGATTATTT TAAAGTGCCC 
TATTCCCTAC CAAAATTAGA TCTGTTAGCT GTGCCTAAAC ATCCCTATGC TGCTATGGAG 
AACTGGGGAC TGAGTGTTTT TGTGGAGCAA AGGATACTAC TGGATCCAAG CATTTCTTCT 
ATATCATACC TGTTGGATGT CACCATGGTC ATTGTACATG AGCTATGTCA TCAGTGGTTT 
GGTGACCTTG TGACTCCAGT TTGGTGGGAG GATGTGTGGT TGAAAGAGGG TTTTGCTCAC 
TATTTTGAAT TTATCGGGAC AGACTACCTC TACCCAGGAT GGAACATGGA AAAGCAGAGA 
TTTCTGACAG ATGTTCTACA TGAAGTGATG TTGCTGGATG GTTTGACCAG TTCGCATCCA 
GTATCCCAGG AGGTGCAGCA AGCCACAGAC ATTGACAGGG TGTTTGACTG GATTGCATAT 
AAAAAGGGTG CAGCTTTAAT TCGAATGTTG GCTAATTTTA TGGGTCACTC TGTGTTTCAA 
ATGGGCTTGC AAGACTATTT AACCATTCAC AAGTATGGCA ATGCAGCCAG AAACGATCTC 
TGGAACACAT TGTCAAAGGC TTTAAAAAGG GTTGGAAAAT CTGTAAATAT CCAAGAAGTA 
ATGGATCAGT GGACGCTACA GATGGGTTAT CCTGTTATCA CCATCTTGGG AAATGAAACT 
GCAGACAATG TCATAGTAAT CTCCCAGGAG CGTTTTGTTT ATGATAGCGA TACTAAAACC 
AAGGATTCTG GCCTTGGAGA CAACAGCTAC TTGTGGCAGA TTCCATTAAC AATTGCAGTA 
GGAAATACGA GCCACATCTC ATCAGAGGCA ATTATATGGG TGTCTAACAA ATCAGAACAC 
CACAGAATTC CTGCTTTGGA AGAGGCAAGT TGGTTGCTGG GAAATATCAA CCAGACTGGC 
TACTTCAGAG TTAATTATGA TATTAGGAAT TGGAGGCTGC TGATTAATCA GCTGACAAGG 
AACCACGAGG TTATCTCTGT CAGCGATCGA GCAGGCTTGA TCGATGATGC ATTCAATCTA 
GCCAGAGCTG GTTATTTGCC TCAGAATATT CCTTTGGAGA TTATCAGATA CCTTTCAGAG 
GAGAAGGATT TTCTTCCTTG GCATGCAGCC AGCCAAGCTC TTTATCCTCT AGATAAATTA 
CTGGACCGCA CAGAAAACTA CAACATCTTC AGTGAGTATA TTTTAAGACA AGTCGCATCA 
ATGTATCTCA AGCTTGGATG GCCAACGAAT AATTTAAACA AATCGCTTGT TCAAGCATCC 
TACCAACATG AAGAGTTGCG TCGGGAAGTC ATCATGTTGG CCTGCAGCTT TGGTAACAAA 
CACTGTCACC AGCAGGCTGC AACATTAATT TCAGATTGGA TTTCTAGCAA TAGAAACCGA 
ATCCCACTTA ATGTGAGAGA CATTGTCTAC TGTACAGGAG TTTCACTGAT GGATGAAGAT 
GTATGGGAGT TCATTTGGAT GAAATTTCAT TCTACATTAG CAGTGTCTGA GAAGAAAATA 
TTATTAGAAG CCTTAACTTG CAGTGATGAC AGAAACTTGC TTAACAGACT TTTGAATTTG 
TCTCTCAACT CAGAAGTTGT CCTGGATCAG GATGCAATTG ATGTGATAAT TCATGTAGCT 
CGAAATCCAC ATGGAAGAGA TCTTGCTTGG AAGTTTTTCA GAGAAAAATG GAAAATATTA 
AATGCCAGGT ATGGAGAAGC ATTATTTATG AATTCGAAGC TTATCAGTGG AGTCACAGAG 
TTCCTCAATA CCGAAGAAGA ACTAAGAGAG CTAAAGAACT TTATAAAGAG TTATGAGGAG 
GGAGCTGCTG CCTCTTTCTC TCGTGCTGTG GAAACTGTGG AAGCCAATGT GCGGTGGCAA 
AGACTTTATA AGGAAGAACT ATTCCAGTGG TTAAGAAAAT CCTTGACACA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRHDE ( XM_015283004.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015138490.1 Gallus gallus thyrotropin-releasing hormone degrading enzyme (TRHDE), mRNA.

Price & Availability↑

CloneID	OGa29215
Clone ID Related Accession (Same CDS sequence)	XM_015283004.1
Accession Version	XM_015283004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	LOW QUALITY PROTEIN: thyrotropin-releasing hormone-degrading ectoenzyme
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455712.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGCCCTGG AGGAGAAGCG GGAGAAGCGG CCGCCCGTCA CCCCCATGAT GATCGAGGAG 
GAGGCAGCCC TGCGGAACGG CAGCCGGGGA CTGCCGCCGG GCCCCTGGGC CGAGGCGGCG 
GGGCCGCGAC CCCGCACGAC GGAGCGGCAC ATCGCCGTGC ACAAGCGCCT GGTGCTGGGC 
TTCGCCGTCT CCATCCTGGC GCTGCTGGCC GTGACCATGG TGGCCGTGCT GCTCAGCGTC 
CGCTTCGAGG AGTGCGGCGC GGCCGCCGAC GGACCCCGCG GGCCGCCANA CAACGGGAGC 
CTGCCGGGAC CCGCGGCCGG GCAGCCCCGC GGCCGGGCCG AGCCGCGGGA GGAGGCGCGG 
GGCGGGGAGG AGGCGGCGGA GCGGCGGCAG GAGGAGGAGG AGGAGGAGGA GGAGGAGTGG 
CAGCGGCGGC GGCGGCTGCC GCCCTGGGCC CGGCCGCGGC TGCCGCGGCA CCTGCGGCCG 
CTGCACTACA ACCTGATGCT GAGCGCCTTC ATGGAGAACT TCACCTTCTG CGGGGAGGTG 
AACGTGCAGC TGGAGGTGCG CAACGCCAGC CGCTACATCG TGCTCCACGC CCACCGCATG 
CACATCGAGG CGGTGCGCGT GGCCGAGGAC AAGCTGGCGG GCGCCGTGCG GGTGGCACGG 
TCCTTCCTCT ACCCCCAGAC CCAGGTCCTG GTCGTCGTCC TCAACCGCAG CCTGGAGGTG 
CAGCGGAGTT ACAACCTCAA GATCATCTAC AACGCGCTCA TCGAGAACGA GCTGCTCGGC 
TTCTTCCGCA GCTCCTACGT CCTGCACGGC GAGCGCAGGT TTCTTGGTGT TACGCAGTTT 
TCTCCTACTC ATGCCAGAAA AGCCTTTCCT TGCTTTGATG AGCCCATCTA CAAGGCCACT 
TTCAAAATCA GCATCAAGCA TCAAGCAACT TATATATCTT TGTCCAACAT GCCAGTTGAA 
ACTTCTGTTT TTGAAGAAGA TGGATGGGTT ACAGATCACT TTTCACAAAC CCCTCTCATG 
TCCACATATT ACCTTGCTTG GGCAGTGTGC AATTTTACGT ACCGGGAGAC CATCACCAAG 
AGTGGAGTGG TTGTACGACT GTACGCAAGG CCTGATGCAA TCCGAAGAGG ATCAGGAGAC 
TATGCCCTAA ATATTACAAG AAGACTCATT GAATTTTATG AAGATTATTT TAAAGTGCCC 
TATTCCCTAC CAAAATTAGA TCTGTTAGCT GTGCCTAAAC ATCCCTATGC TGCTATGGAG 
AACTGGGGAC TGAGTGTTTT TGTGGAGCAA AGGATACTAC TGGATCCAAG CATTTCTTCT 
ATATCATACC TGTTGGATGT CACCATGGTC ATTGTACATG AGCTATGTCA TCAGTGGTTT 
GGTGACCTTG TGACTCCAGT TTGGTGGGAG GATGTGTGGT TGAAAGAGGG TTTTGCTCAC 
TATTTTGAAT TTATCGGGAC AGACTACCTC TACCCAGGAT GGAACATGGA AAAGCAGAGA 
TTTCTGACAG ATGTTCTACA TGAAGTGATG TTGCTGGATG GTTTGACCAG TTCGCATCCA 
GTATCCCAGG AGGTGCAGCA AGCCACAGAC ATTGACAGGG TGTTTGACTG GATTGCATAT 
AAAAAGGGTG CAGCTTTAAT TCGAATGTTG GCTAATTTTA TGGGTCACTC TGTGTTTCAA 
ATGGGCTTGC AAGACTATTT AACCATTCAC AAGTATGGCA ATGCAGCCAG AAACGATCTC 
TGGAACACAT TGTCAAAGGC TTTAAAAAGG GTTGGAAAAT CTGTAAATAT CCAAGAAGTA 
ATGGATCAGT GGACGCTACA GATGGGTTAT CCTGTTATCA CCATCTTGGG AAATGAAACT 
GCAGACAATG TCATAGTAAT CTCCCAGGAG CGTTTTGTTT ATGATAGCGA TACTAAAACC 
AAGGATTCTG GCCTTGGAGA CAACAGCTAC TTGTGGCAGA TTCCATTAAC AATTGCAGTA 
GGAAATACGA GCCACATCTC ATCAGAGGCA ATTATATGGG TGTCTAACAA ATCAGAACAC 
CACAGAATTC CTGCTTTGGA AGAGGCAAGT TGGTTGCTGG GAAATATCAA CCAGACTGGC 
TACTTCAGAG TTAATTATGA TATTAGGAAT TGGAGGCTGC TGATTAATCA GCTGACAAGG 
AACCACGAGG TTATCTCTGT CAGCGATCGA GCAGGCTTGA TCGATGATGC ATTCAATCTA 
GCCAGAGCTG GTTATTTGCC TCAGAATATT CCTTTGGAGA TTATCAGATA CCTTTCAGAG 
GAGAAGGATT TTCTTCCTTG GCATGCAGCC AGCCAAGCTC TTTATCCTCT AGATAAATTA 
CTGGACCGCA CAGAAAACTA CAACATCTTC AGTGAGTATA TTTTAAGACA AGTCGCATCA 
ATGTATCTCA AGCTTGGATG GCCAACGAAT AATTTAAACA AATCGCTTGT TCAAGCATCC 
TACCAACATG AAGAGTTGCG TCGGGAAGTC ATCATGTTGG CCTGCAGCTT TGGTAACAAA 
CACTGTCACC AGCAGGCTGC AACATTAATT TCAGATTGGA TTTCTAGCAA TAGAAACCGA 
ATCCCACTTA ATGTGAGAGA CATTGTCTAC TGTACAGGAG TTTCACTGAT GGATGAAGAT 
GTATGGGAGT TCATTTGGAT GAAATTTCAT TCTACATTAG CAGTGTCTGA GAAGAAAATA 
TTATTAGAAG CCTTAACTTG CAGTGATGAC AGAAACTTGC TTAACAGACT TTTGAATTTG 
TCTCTCAACT CAGAAGTTGT CCTGGATCAG GATGCAATTG ATGTGATAAT TCATGTAGCT 
CGAAATCCAC ATGGAAGAGA TCTTGCTTGG AAGTTTTTCA GAGAAAAATG GAAAATATTA 
AATGCCAGGT ATGGAGAAGC ATTATTTATG AATTCGAAGC TTATCAGTGG AGTCACAGAG 
TTCCTCAATA CCGAAGAAGA ACTAAGAGAG CTAAAGAACT TTATAAAGAG TTATGAGGAG 
GGAGCTGCTG CCTCTTTCTC TCGTGCTGTG GAAACTGTGG AAGCCAATGT GCGGTGGCAA 
AGACTTTATA AGGAAGAACT ATTCCAGTGG TTAAGAAAAT CCTTGACACA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015138490.1
CDS	20..3133
Translation	MALEEKREKRPPVTPMMIEEEAALRNGSRGLPPGPWAEAAGPRPRTTERHIAVHKRLVLGFAVSILALLAVTMVAVLLSVRFEECGAAADGPRGPPnullNGSLPGPAAGQPRGRAEPREEARGGEEAAERRQEEEEEEEEEWQRRRRLPPWARPRLPRHLRPLHYNLMLSAFMENFTFCGEVNVQLEVRNASRYIVLHAHRMHIEAVRVAEDKLAGAVRVARSFLYPQTQVLVVVLNRSLEVQRSYNLKIIYNALIENELLGFFRSSYVLHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYISLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAVCNFTYRETITKSGVVVRLYARPDAIRRGSGDYALNITRRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSVFVEQRILLDPSISSISYLLDVTMVIVHELCHQWFGDLVTPVWWEDVWLKEGFAHYFEFIGTDYLYPGWNMEKQRFLTDVLHEVMLLDGLTSSHPVSQEVQQATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQMGLQDYLTIHKYGNAARNDLWNTLSKALKRVGKSVNIQEVMDQWTLQMGYPVITILGNETADNVIVISQERFVYDSDTKTKDSGLGDNSYLWQIPLTIAVGNTSHISSEAIIWVSNKSEHHRIPALEEASWLLGNINQTGYFRVNYDIRNWRLLINQLTRNHEVISVSDRAGLIDDAFNLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASQALYPLDKLLDRTENYNIFSEYILRQVASMYLKLGWPTNNLNKSLVQASYQHEELRREVIMLACSFGNKHCHQQAATLISDWISSNRNRIPLNVRDIVYCTGVSLMDEDVWEFIWMKFHSTLAVSEKKILLEALTCSDDRNLLNRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFREKWKILNARYGEALFMNSKLISGVTEFLNTEEELRELKNFIKSYEEGAAASFSRAVETVEANVRWQRLYKEELFQWLRKSLTH

Target ORF information:

RefSeq Version	XM_015283004.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus thyrotropin-releasing hormone degrading enzyme (TRHDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283004.1

ORF Insert Sequence:

ATGGCCCTGG AGGAGAAGCG GGAGAAGCGG CCGCCCGTCA CCCCCATGAT GATCGAGGAG 
GAGGCAGCCC TGCGGAACGG CAGCCGGGGA CTGCCGCCGG GCCCCTGGGC CGAGGCGGCG 
GGGCCGCGAC CCCGCACGAC GGAGCGGCAC ATCGCCGTGC ACAAGCGCCT GGTGCTGGGC 
TTCGCCGTCT CCATCCTGGC GCTGCTGGCC GTGACCATGG TGGCCGTGCT GCTCAGCGTC 
CGCTTCGAGG AGTGCGGCGC GGCCGCCGAC GGACCCCGCG GGCCGCCANA CAACGGGAGC 
CTGCCGGGAC CCGCGGCCGG GCAGCCCCGC GGCCGGGCCG AGCCGCGGGA GGAGGCGCGG 
GGCGGGGAGG AGGCGGCGGA GCGGCGGCAG GAGGAGGAGG AGGAGGAGGA GGAGGAGTGG 
CAGCGGCGGC GGCGGCTGCC GCCCTGGGCC CGGCCGCGGC TGCCGCGGCA CCTGCGGCCG 
CTGCACTACA ACCTGATGCT GAGCGCCTTC ATGGAGAACT TCACCTTCTG CGGGGAGGTG 
AACGTGCAGC TGGAGGTGCG CAACGCCAGC CGCTACATCG TGCTCCACGC CCACCGCATG 
CACATCGAGG CGGTGCGCGT GGCCGAGGAC AAGCTGGCGG GCGCCGTGCG GGTGGCACGG 
TCCTTCCTCT ACCCCCAGAC CCAGGTCCTG GTCGTCGTCC TCAACCGCAG CCTGGAGGTG 
CAGCGGAGTT ACAACCTCAA GATCATCTAC AACGCGCTCA TCGAGAACGA GCTGCTCGGC 
TTCTTCCGCA GCTCCTACGT CCTGCACGGC GAGCGCAGGT TTCTTGGTGT TACGCAGTTT 
TCTCCTACTC ATGCCAGAAA AGCCTTTCCT TGCTTTGATG AGCCCATCTA CAAGGCCACT 
TTCAAAATCA GCATCAAGCA TCAAGCAACT TATATATCTT TGTCCAACAT GCCAGTTGAA 
ACTTCTGTTT TTGAAGAAGA TGGATGGGTT ACAGATCACT TTTCACAAAC CCCTCTCATG 
TCCACATATT ACCTTGCTTG GGCAGTGTGC AATTTTACGT ACCGGGAGAC CATCACCAAG 
AGTGGAGTGG TTGTACGACT GTACGCAAGG CCTGATGCAA TCCGAAGAGG ATCAGGAGAC 
TATGCCCTAA ATATTACAAG AAGACTCATT GAATTTTATG AAGATTATTT TAAAGTGCCC 
TATTCCCTAC CAAAATTAGA TCTGTTAGCT GTGCCTAAAC ATCCCTATGC TGCTATGGAG 
AACTGGGGAC TGAGTGTTTT TGTGGAGCAA AGGATACTAC TGGATCCAAG CATTTCTTCT 
ATATCATACC TGTTGGATGT CACCATGGTC ATTGTACATG AGCTATGTCA TCAGTGGTTT 
GGTGACCTTG TGACTCCAGT TTGGTGGGAG GATGTGTGGT TGAAAGAGGG TTTTGCTCAC 
TATTTTGAAT TTATCGGGAC AGACTACCTC TACCCAGGAT GGAACATGGA AAAGCAGAGA 
TTTCTGACAG ATGTTCTACA TGAAGTGATG TTGCTGGATG GTTTGACCAG TTCGCATCCA 
GTATCCCAGG AGGTGCAGCA AGCCACAGAC ATTGACAGGG TGTTTGACTG GATTGCATAT 
AAAAAGGGTG CAGCTTTAAT TCGAATGTTG GCTAATTTTA TGGGTCACTC TGTGTTTCAA 
ATGGGCTTGC AAGACTATTT AACCATTCAC AAGTATGGCA ATGCAGCCAG AAACGATCTC 
TGGAACACAT TGTCAAAGGC TTTAAAAAGG GTTGGAAAAT CTGTAAATAT CCAAGAAGTA 
ATGGATCAGT GGACGCTACA GATGGGTTAT CCTGTTATCA CCATCTTGGG AAATGAAACT 
GCAGACAATG TCATAGTAAT CTCCCAGGAG CGTTTTGTTT ATGATAGCGA TACTAAAACC 
AAGGATTCTG GCCTTGGAGA CAACAGCTAC TTGTGGCAGA TTCCATTAAC AATTGCAGTA 
GGAAATACGA GCCACATCTC ATCAGAGGCA ATTATATGGG TGTCTAACAA ATCAGAACAC 
CACAGAATTC CTGCTTTGGA AGAGGCAAGT TGGTTGCTGG GAAATATCAA CCAGACTGGC 
TACTTCAGAG TTAATTATGA TATTAGGAAT TGGAGGCTGC TGATTAATCA GCTGACAAGG 
AACCACGAGG TTATCTCTGT CAGCGATCGA GCAGGCTTGA TCGATGATGC ATTCAATCTA 
GCCAGAGCTG GTTATTTGCC TCAGAATATT CCTTTGGAGA TTATCAGATA CCTTTCAGAG 
GAGAAGGATT TTCTTCCTTG GCATGCAGCC AGCCAAGCTC TTTATCCTCT AGATAAATTA 
CTGGACCGCA CAGAAAACTA CAACATCTTC AGTGAGTATA TTTTAAGACA AGTCGCATCA 
ATGTATCTCA AGCTTGGATG GCCAACGAAT AATTTAAACA AATCGCTTGT TCAAGCATCC 
TACCAACATG AAGAGTTGCG TCGGGAAGTC ATCATGTTGG CCTGCAGCTT TGGTAACAAA 
CACTGTCACC AGCAGGCTGC AACATTAATT TCAGATTGGA TTTCTAGCAA TAGAAACCGA 
ATCCCACTTA ATGTGAGAGA CATTGTCTAC TGTACAGGAG TTTCACTGAT GGATGAAGAT 
GTATGGGAGT TCATTTGGAT GAAATTTCAT TCTACATTAG CAGTGTCTGA GAAGAAAATA 
TTATTAGAAG CCTTAACTTG CAGTGATGAC AGAAACTTGC TTAACAGACT TTTGAATTTG 
TCTCTCAACT CAGAAGTTGT CCTGGATCAG GATGCAATTG ATGTGATAAT TCATGTAGCT 
CGAAATCCAC ATGGAAGAGA TCTTGCTTGG AAGTTTTTCA GAGAAAAATG GAAAATATTA 
AATGCCAGGT ATGGAGAAGC ATTATTTATG AATTCGAAGC TTATCAGTGG AGTCACAGAG 
TTCCTCAATA CCGAAGAAGA ACTAAGAGAG CTAAAGAACT TTATAAAGAG TTATGAGGAG 
GGAGCTGCTG CCTCTTTCTC TCGTGCTGTG GAAACTGTGG AAGCCAATGT GCGGTGGCAA 
AGACTTTATA AGGAAGAACT ATTCCAGTGG TTAAGAAAAT CCTTGACACA CTAA