Trhde cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Trhde
Entrez Gene ID	237553
Full Name	TRH-degrading enzyme
Synonyms	9330155P21Rik
General protein information	Preferred Names TRH-degrading enzyme Names thyrotropin-releasing hormone-degrading ectoenzyme PAP-II TRH-DE TRH-degrading ectoenzyme TRH-specific aminopeptidase pyroglutamyl-peptidase II thyroliberinase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10\|10 D2

mRNA and Protein(s)

mRNA	Protein	Name
NM_146241.2	NP_666353.1	thyrotropin-releasing hormone-degrading ectoenzyme
NM_146241.3	NP_666353.2	thyrotropin-releasing hormone-degrading ectoenzyme

Pathways from BioSystems

Homologies

Homo sapiens (human)	TRHDE	NP_037513.1
Pan troglodytes (chimpanzee)	TRHDE	XP_522471.4
Canis lupus familiaris (dog)	TRHDE	XP_005625720.1
Xenopus tropicalis (tropical clawed frog)	trhde	XP_002939988.2
	TRHDE	XP_001109391.2
Bos taurus (cattle)	TRHDE	XP_002687216.3
Rattus norvegicus (Norway rat)	Trhde	NP_001102461.1
Mus musculus (house mouse)	Trhde	NP_666353.1
Gallus gallus (chicken)	TRHDE	XP_425442.3
Danio rerio (zebrafish)	LOC562409	XP_690889.2
Danio rerio (zebrafish)	si:ch211-149p5.1	XP_001345457.3

Gene Ontology
Bibliography

Related articles in PubMed

A mouse knockout library for secreted and transmembrane proteins.
Tang T, Li L, Tang J, Li Y, Lin WY, Martin F, Grant D, Solloway M, Parker L, Ye W, Forrest W, Ghilardi N, Oravecz T, Platt KA, Rice DS, Hansen GM, Abuin A, Eberhart DE, Godowski P, Holt KH, Peterson A, Zambrowicz BP, de Sauvage FJ
Nature biotechnology28(7)749-55(2010 Jul)

Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platform.
Yaylaoglu MB, Titmus A, Visel A, Alvarez-Bolado G, Thaller C, Eichele G
Developmental dynamics : an official publication of the American Association of Anatomists234(2)371-86(2005 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Trhde gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Trhde cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu12359	NM_146241.2 Latest version!	Mus musculus TRH-degrading enzyme (Trhde), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$559.30 $799.00
OMu90562	NM_146241.3 Latest version!	Mus musculus TRH-degrading enzyme (Trhde), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Trhde ( NM_146241.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666353.1 Mus musculus TRH-degrading enzyme (Trhde), mRNA.

Price & Availability↑

CloneID	OMu12359
Clone ID Related Accession (Same CDS sequence)	NM_146241.2
Accession Version	NM_146241.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	thyrotropin-releasing hormone-degrading ectoenzyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC160408.6, AK034093.1 and AC124558.4. On Aug 25, 2009 this sequence version replaced NM_146241.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC032288.1, SRR1660821.25076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164131 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGAAG ACGACGCCGC GCTCCGGGCA AGCGGCAGGG GGCTCTCGGA CCCGTGGGCT 
GACTCGGTGG GAGTGAGACC CCGCACCACG GAGCGCCACA TCGCAGTGCA CAAGAGGCTT 
GTGCTGGCCT TTGCCGTGTC CATCGTGGCA CTGCTGGCTG TCACCATGCT GGCCGTCCTG 
CTCAGCTTGC GATTCGACGA GTGTGGAGCG AGCGCGGCGA TGCCTGGCAC CGACGGTGGC 
CTCGGGGGTT TCCCCGAGCG CGACAGCAAC AGCAGCTTTC CAGGTTCTGC CCGGCGCAAC 
CACCACGCGG GTGGGGAATC CTCTCAGCGT GAGAGCGGCG AGGTGGGCAC CCCGGGGACC 
CCGTCTGCCC AGCCGCCGTC GGAGGAAGAG CGGGAGCAGT GGCAGCCCTG GACACAGCTG 
CGCCTATCCG GCCACCTCAA GCCGCTGCAC TACAATTTGA TGCTCACCGC TTTCATGGAG 
AACTTCACCT TCTCTGGGGA GGTCAACGTG GAGATCGCGT GCCGGAACGC CACCCGCTAC 
GTGGTACTGC ACGCCTCCCG AGTGGCGGTG GAGAAGGTGC AAGTAGCTGA AGACAGGGCG 
TTCGGGGCTG TCCCGGTTGC AGGCTTTTTC CTCTACCCAC AAACGCAGGT CTTGGTGGTG 
GTATTGAACA GAACCCTGGA CGCGCAGAGG CATTACAATC TGAAGATTAT CTACAATGCC 
CTGATAGAGA ACGAGCTTTT GGGCTTCTTC CGCAGCTCCT ACGTGATCCA CGGGGAGAGA 
AGATTCCTTG GTGTTACTCA GTTTTCACCT ACACATGCCA GGAAGGCGTT TCCGTGTTTC 
GATGAACCAA TCTACAAGGC CACTTTCAAA ATCAGCATCA AACATCAAGC AACCTATTTG 
TCTCTATCCA ACATGCCAGT GGAAACGTCT GTGTTCGAGG AAGATGGATG GGTGACAGAT 
CACTTTTCAC AGACCCCTCT CATGTCTACA TATTATTTAG CCTGGGCGAT TTGCAACTTC 
ACATACAGAG AAACTACCAC CAAGAGTGGG GTTGTAGTCC GATTATATGC GAGACCCGAC 
GCTATCAGAA GAGGATCCGG GGACTATGCT CTCCACATTA CAAAGAGATT AATAGAATTT 
TATGAAGACT ACTTTAAAGT GCCCTATTCT TTGCCAAAAC TAGATCTTTT AGCTGTGCCT 
AAGCATCCTT ATGCTGCTAT GGAGAACTGG GGACTAAGTA TTTTTGTGGA ACAAAGAATA 
CTGCTGGATC CCAGTGTGTC ATCTATTTCT TATTTGCTGG ATGTCACCAT GGTCATTGTT 
CATGAAATAT GTCACCAGTG GTTTGGTGAC CTTGTGACTC CAGTTTGGTG GGAAGATGTG 
TGGTTGAAGG AAGGCTTTGC TCACTACTTT GAATTTGTGG GTACAGACTA CCTCTACCCT 
GCCTGGAACA TGGAGAAGCA GAGATTTCTG ACAGATGTTC TGCATGAAGT GATGCTACTT 
GATGGCTTGG CCAGTTCCCA TCCAGTATCA CAGGAAGTGC TACGGGCAAC AGATATTGAC 
AGAGTGTTCG ACTGGATCGC ATACAAAAAG GGTGCTGCTC TAATTAGAAT GCTGGCTAAT 
TTTATGGGTC ATTCAGTTTT TCAGAGGGGA TTGCAAGATT ATTTAACCAT TCACAAGTAT 
GGCAATGCAG CCAGAAATGA TCTCTGGAAT ACATTATCAG AGGCTTTAAG AAGGAATGGA 
AAATACGTGA ACATACAAGA AGTAATGGAT CAGTGGACAC TGCAGATGGG ATATCCTGTT 
ATCACTATCC TGGGAAACAC GACGGCAGAA AATAGAATAC TTATCACCCA ACAGCATTTC 
ATTTATGACA TTGGTGCTAA AACTAAAGCA CTTCAACTTC AAAACAGCAG TTACCTGTGG 
CAGATTCCAT TAACCATTGT GGTAGGAAAT AGAAGCCATG TGTCTTCAGA AGCAATTATT 
TGGGTGTCTA ACAAATCAGA ACATCACAGA ATAGCGTACT TAGACAGAGG AAGCTGGATC 
CTTGGGAACA TCAATCAAAC CGGCTACTTT AGAGTCAACT ATGACCTAAG GAACTGGAGA 
TTACTAATCG ATCAGTTAAT CAGGAACCAC GAGGTACTTT CTGTCAGTAA CCGCGCGGCT 
TTGATCGATG ATGCCTTCAG CCTGGCCAGG GCAGGCTATT TGCCTCAGAA TATCCCCCTA 
GAGATCATCA GATACCTGTC TGAGGAAAAG GATTTTCTTC CTTGGCACGC TGCCAGCCGA 
GCTCTTTATC CTCTGGATAA ATTACTGGAC CGCATGGAGA ACTACAATAT CTTCAATGAA 
TATATTCTAA AACAAGTTGC AACAACATAC ATCAAGCTTG GATGGCCAAG AAATAACTTC 
AATGGATCTC TCGTTCAAGC CTCCTACCAA CATGAGGAGC TACGGAGGGA AGTGATAATG 
TTGGCATGTA GTTTTGGGAA CAAGCACTGT CACCAGCAGG CATCAACACT TATTTCAGAC 
TGGATTTCCA GCAACAGAAA CAGAATACCC CTGAATGTTA GAGACATCGT CTACTGCACA 
GGAGTGTCAC TGCTGGATGA GGATGTCTGG GAGTTTATCT GGATGAAATT CCACTCTACC 
ACTGCAGTTT CTGAGAAAAA GATACTGCTG GAGGCTCTAA CTTGCAGCGA TGACAGGAAT 
TTATTAAGTA GGCTTCTAAA TCTGTCTCTG AATTCTGAGG TGGTGTTGGA TCAAGATGCA 
ATTGATGTAA TTATCCACGT AGCCAGAAAT CCACATGGCC GAGACCTTGC CTGGAAGTTT 
TTCAGAGACA AATGGAAAAT ACTAAATACC AGGTATGGAG AAGCACTGTT TATGAATTCC 
AAACTTATCA GTGGAGTTAC AGAATTTCTT AATACAGAAG GTGAACTTAA AGAGCTAAAG 
AATTTCATGA AGTCCTATGA TGGGGTAGCC TCTGCTTCTT TCTCACGAGC TGTGGAAACT 
GTGGAAGCCA ATGTGCGCTG GAAAAGGTTT TACCAAGATG AGCTTTTCCA GTGGCTGGGA 
AAAGCCATGA GACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666353.1
CDS	71..3148
Translation	MGEDDAALRASGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSIVALLAVTMLAVLLSLRFDECGASAAMPGTDGGLGGFPERDSNSSFPGSARRNHHAGGESSQRESGEVGTPGTPSAQPPSEEEREQWQPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQVAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRHYNLKIIYNALIENELLGFFRSSYVIHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPAWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLRATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALRRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRILITQQHFIYDIGAKTKALQLQNSSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRIAYLDRGSWILGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAALIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPRNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLSRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKSYDGVASASFSRAVETVEANVRWKRFYQDELFQWLGKAMRH

Target ORF information:

RefSeq Version	NM_146241.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus TRH-degrading enzyme (Trhde), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146241.2

ORF Insert Sequence:

ATGGGAGAAG ACGACGCCGC GCTCCGGGCA AGCGGCAGGG GGCTCTCGGA CCCGTGGGCT 
GACTCGGTGG GAGTGAGACC CCGCACCACG GAGCGCCACA TCGCAGTGCA CAAGAGGCTT 
GTGCTGGCCT TTGCCGTGTC CATCGTGGCA CTGCTGGCTG TCACCATGCT GGCCGTCCTG 
CTCAGCTTGC GATTCGACGA GTGTGGAGCG AGCGCGGCGA TGCCTGGCAC CGACGGTGGC 
CTCGGGGGTT TCCCCGAGCG CGACAGCAAC AGCAGCTTTC CAGGTTCTGC CCGGCGCAAC 
CACCACGCGG GTGGGGAATC CTCTCAGCGT GAGAGCGGCG AGGTGGGCAC CCCGGGGACC 
CCGTCTGCCC AGCCGCCGTC GGAGGAAGAG CGGGAGCAGT GGCAGCCCTG GACACAGCTG 
CGCCTATCCG GCCACCTCAA GCCGCTGCAC TACAATTTGA TGCTCACCGC TTTCATGGAG 
AACTTCACCT TCTCTGGGGA GGTCAACGTG GAGATCGCGT GCCGGAACGC CACCCGCTAC 
GTGGTACTGC ACGCCTCCCG AGTGGCGGTG GAGAAGGTGC AAGTAGCTGA AGACAGGGCG 
TTCGGGGCTG TCCCGGTTGC AGGCTTTTTC CTCTACCCAC AAACGCAGGT CTTGGTGGTG 
GTATTGAACA GAACCCTGGA CGCGCAGAGG CATTACAATC TGAAGATTAT CTACAATGCC 
CTGATAGAGA ACGAGCTTTT GGGCTTCTTC CGCAGCTCCT ACGTGATCCA CGGGGAGAGA 
AGATTCCTTG GTGTTACTCA GTTTTCACCT ACACATGCCA GGAAGGCGTT TCCGTGTTTC 
GATGAACCAA TCTACAAGGC CACTTTCAAA ATCAGCATCA AACATCAAGC AACCTATTTG 
TCTCTATCCA ACATGCCAGT GGAAACGTCT GTGTTCGAGG AAGATGGATG GGTGACAGAT 
CACTTTTCAC AGACCCCTCT CATGTCTACA TATTATTTAG CCTGGGCGAT TTGCAACTTC 
ACATACAGAG AAACTACCAC CAAGAGTGGG GTTGTAGTCC GATTATATGC GAGACCCGAC 
GCTATCAGAA GAGGATCCGG GGACTATGCT CTCCACATTA CAAAGAGATT AATAGAATTT 
TATGAAGACT ACTTTAAAGT GCCCTATTCT TTGCCAAAAC TAGATCTTTT AGCTGTGCCT 
AAGCATCCTT ATGCTGCTAT GGAGAACTGG GGACTAAGTA TTTTTGTGGA ACAAAGAATA 
CTGCTGGATC CCAGTGTGTC ATCTATTTCT TATTTGCTGG ATGTCACCAT GGTCATTGTT 
CATGAAATAT GTCACCAGTG GTTTGGTGAC CTTGTGACTC CAGTTTGGTG GGAAGATGTG 
TGGTTGAAGG AAGGCTTTGC TCACTACTTT GAATTTGTGG GTACAGACTA CCTCTACCCT 
GCCTGGAACA TGGAGAAGCA GAGATTTCTG ACAGATGTTC TGCATGAAGT GATGCTACTT 
GATGGCTTGG CCAGTTCCCA TCCAGTATCA CAGGAAGTGC TACGGGCAAC AGATATTGAC 
AGAGTGTTCG ACTGGATCGC ATACAAAAAG GGTGCTGCTC TAATTAGAAT GCTGGCTAAT 
TTTATGGGTC ATTCAGTTTT TCAGAGGGGA TTGCAAGATT ATTTAACCAT TCACAAGTAT 
GGCAATGCAG CCAGAAATGA TCTCTGGAAT ACATTATCAG AGGCTTTAAG AAGGAATGGA 
AAATACGTGA ACATACAAGA AGTAATGGAT CAGTGGACAC TGCAGATGGG ATATCCTGTT 
ATCACTATCC TGGGAAACAC GACGGCAGAA AATAGAATAC TTATCACCCA ACAGCATTTC 
ATTTATGACA TTGGTGCTAA AACTAAAGCA CTTCAACTTC AAAACAGCAG TTACCTGTGG 
CAGATTCCAT TAACCATTGT GGTAGGAAAT AGAAGCCATG TGTCTTCAGA AGCAATTATT 
TGGGTGTCTA ACAAATCAGA ACATCACAGA ATAGCGTACT TAGACAGAGG AAGCTGGATC 
CTTGGGAACA TCAATCAAAC CGGCTACTTT AGAGTCAACT ATGACCTAAG GAACTGGAGA 
TTACTAATCG ATCAGTTAAT CAGGAACCAC GAGGTACTTT CTGTCAGTAA CCGCGCGGCT 
TTGATCGATG ATGCCTTCAG CCTGGCCAGG GCAGGCTATT TGCCTCAGAA TATCCCCCTA 
GAGATCATCA GATACCTGTC TGAGGAAAAG GATTTTCTTC CTTGGCACGC TGCCAGCCGA 
GCTCTTTATC CTCTGGATAA ATTACTGGAC CGCATGGAGA ACTACAATAT CTTCAATGAA 
TATATTCTAA AACAAGTTGC AACAACATAC ATCAAGCTTG GATGGCCAAG AAATAACTTC 
AATGGATCTC TCGTTCAAGC CTCCTACCAA CATGAGGAGC TACGGAGGGA AGTGATAATG 
TTGGCATGTA GTTTTGGGAA CAAGCACTGT CACCAGCAGG CATCAACACT TATTTCAGAC 
TGGATTTCCA GCAACAGAAA CAGAATACCC CTGAATGTTA GAGACATCGT CTACTGCACA 
GGAGTGTCAC TGCTGGATGA GGATGTCTGG GAGTTTATCT GGATGAAATT CCACTCTACC 
ACTGCAGTTT CTGAGAAAAA GATACTGCTG GAGGCTCTAA CTTGCAGCGA TGACAGGAAT 
TTATTAAGTA GGCTTCTAAA TCTGTCTCTG AATTCTGAGG TGGTGTTGGA TCAAGATGCA 
ATTGATGTAA TTATCCACGT AGCCAGAAAT CCACATGGCC GAGACCTTGC CTGGAAGTTT 
TTCAGAGACA AATGGAAAAT ACTAAATACC AGGTATGGAG AAGCACTGTT TATGAATTCC 
AAACTTATCA GTGGAGTTAC AGAATTTCTT AATACAGAAG GTGAACTTAA AGAGCTAAAG 
AATTTCATGA AGTCCTATGA TGGGGTAGCC TCTGCTTCTT TCTCACGAGC TGTGGAAACT 
GTGGAAGCCA ATGTGCGCTG GAAAAGGTTT TACCAAGATG AGCTTTTCCA GTGGCTGGGA 
AAAGCCATGA GACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Trhde ( NM_146241.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666353.2 Mus musculus TRH-degrading enzyme (Trhde), mRNA.

Price & Availability↑

CloneID	OMu90562
Clone ID Related Accession (Same CDS sequence)	NM_146241.3
Accession Version	NM_146241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Mus musculus(house mouse)
Product	thyrotropin-releasing hormone-degrading ectoenzyme
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC160408.6, BC032288.1 and AC124558.4. On Jul 26, 2019 this sequence version replaced NM_146241.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

ATGGCCCTGG ACGGCGAGAG GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAGAAA 
AAGAAGAGGA AGAAGAAGGA GGAGGAGGGG GCAGAGAAGA GCAGTTCACC CTTTGCGGCC 
ACCATGGGAG AAGACGACGC CGCGCTCCGG GCAAGCGGCA GGGGGCTCTC GGACCCGTGG 
GCTGACTCGG TGGGAGTGAG ACCCCGCACC ACGGAGCGCC ACATCGCAGT GCACAAGAGG 
CTTGTGCTGG CCTTTGCCGT GTCCATCGTG GCACTGCTGG CTGTCACCAT GCTGGCCGTC 
CTGCTCAGCT TGCGATTCGA CGAGTGTGGA GCGAGCGCGG CGATGCCTGG CACCGACGGT 
GGCCTCGGGG GTTTCCCCGA GCGCGACAGC AACAGCAGCT TTCCAGGTTC TGCCCGGCGC 
AACCACCACG CGGGTGGGGA ATCCTCTCAG CGTGAGAGCG GCGAGGTGGG CACCCCGGGG 
ACCCCGTCTG CCCAGCCGCC GTCGGAGGAA GAGCGGGAGC AGTGGCAGCC CTGGACACAG 
CTGCGCCTAT CCGGCCACCT CAAGCCGCTG CACTACAATT TGATGCTCAC CGCTTTCATG 
GAGAACTTCA CCTTCTCTGG GGAGGTCAAC GTGGAGATCG CGTGCCGGAA CGCCACCCGC 
TACGTGGTAC TGCACGCCTC CCGAGTGGCG GTGGAGAAGG TGCAAGTAGC TGAAGACAGG 
GCGTTCGGGG CTGTCCCGGT TGCAGGCTTT TTCCTCTACC CACAAACGCA GGTCTTGGTG 
GTGGTATTGA ACAGAACCCT GGACGCGCAG AGGCATTACA ATCTGAAGAT TATCTACAAT 
GCCCTGATAG AGAACGAGCT TTTGGGCTTC TTCCGCAGCT CCTACGTGAT CCACGGGGAG 
AGAAGATTCC TTGGTGTTAC TCAGTTTTCA CCTACACATG CCAGGAAGGC GTTTCCGTGT 
TTCGATGAAC CAATCTACAA GGCCACTTTC AAAATCAGCA TCAAACATCA AGCAACCTAT 
TTGTCTCTAT CCAACATGCC AGTGGAAACG TCTGTGTTCG AGGAAGATGG ATGGGTGACA 
GATCACTTTT CACAGACCCC TCTCATGTCT ACATATTATT TAGCCTGGGC GATTTGCAAC 
TTCACATACA GAGAAACTAC CACCAAGAGT GGGGTTGTAG TCCGATTATA TGCGAGACCC 
GACGCTATCA GAAGAGGATC CGGGGACTAT GCTCTCCACA TTACAAAGAG ATTAATAGAA 
TTTTATGAAG ACTACTTTAA AGTGCCCTAT TCTTTGCCAA AACTAGATCT TTTAGCTGTG 
CCTAAGCATC CTTATGCTGC TATGGAGAAC TGGGGACTAA GTATTTTTGT GGAACAAAGA 
ATACTGCTGG ATCCCAGTGT GTCATCTATT TCTTATTTGC TGGATGTCAC CATGGTCATT 
GTTCATGAAA TATGTCACCA GTGGTTTGGT GACCTTGTGA CTCCAGTTTG GTGGGAAGAT 
GTGTGGTTGA AGGAAGGCTT TGCTCACTAC TTTGAATTTG TGGGTACAGA CTACCTCTAC 
CCTGCCTGGA ACATGGAGAA GCAGAGATTT CTGACAGATG TTCTGCATGA AGTGATGCTA 
CTTGATGGCT TGGCCAGTTC CCATCCAGTA TCACAGGAAG TGCTACGGGC AACAGATATT 
GACAGAGTGT TCGACTGGAT CGCATACAAA AAGGGTGCTG CTCTAATTAG AATGCTGGCT 
AATTTTATGG GTCATTCAGT TTTTCAGAGG GGATTGCAAG ATTATTTAAC CATTCACAAG 
TATGGCAATG CAGCCAGAAA TGATCTCTGG AATACATTAT CAGAGGCTTT AAGAAGGAAT 
GGAAAATACG TGAACATACA AGAAGTAATG GATCAGTGGA CACTGCAGAT GGGATATCCT 
GTTATCACTA TCCTGGGAAA CACGACGGCA GAAAATAGAA TACTTATCAC CCAACAGCAT 
TTCATTTATG ACATTGGTGC TAAAACTAAA GCACTTCAAC TTCAAAACAG CAGTTACCTG 
TGGCAGATTC CATTAACCAT TGTGGTAGGA AATAGAAGCC ATGTGTCTTC AGAAGCAATT 
ATTTGGGTGT CTAACAAATC AGAACATCAC AGAATAGCGT ACTTAGACAG AGGAAGCTGG 
ATCCTTGGGA ACATCAATCA AACCGGCTAC TTTAGAGTCA ACTATGACCT AAGGAACTGG 
AGATTACTAA TCGATCAGTT AATCAGGAAC CACGAGGTAC TTTCTGTCAG TAACCGCGCG 
GCTTTGATCG ATGATGCCTT CAGCCTGGCC AGGGCAGGCT ATTTGCCTCA GAATATCCCC 
CTAGAGATCA TCAGATACCT GTCTGAGGAA AAGGATTTTC TTCCTTGGCA CGCTGCCAGC 
CGAGCTCTTT ATCCTCTGGA TAAATTACTG GACCGCATGG AGAACTACAA TATCTTCAAT 
GAATATATTC TAAAACAAGT TGCAACAACA TACATCAAGC TTGGATGGCC AAGAAATAAC 
TTCAATGGAT CTCTCGTTCA AGCCTCCTAC CAACATGAGG AGCTACGGAG GGAAGTGATA 
ATGTTGGCAT GTAGTTTTGG GAACAAGCAC TGTCACCAGC AGGCATCAAC ACTTATTTCA 
GACTGGATTT CCAGCAACAG AAACAGAATA CCCCTGAATG TTAGAGACAT CGTCTACTGC 
ACAGGAGTGT CACTGCTGGA TGAGGATGTC TGGGAGTTTA TCTGGATGAA ATTCCACTCT 
ACCACTGCAG TTTCTGAGAA AAAGATACTG CTGGAGGCTC TAACTTGCAG CGATGACAGG 
AATTTATTAA GTAGGCTTCT AAATCTGTCT CTGAATTCTG AGGTGGTGTT GGATCAAGAT 
GCAATTGATG TAATTATCCA CGTAGCCAGA AATCCACATG GCCGAGACCT TGCCTGGAAG 
TTTTTCAGAG ACAAATGGAA AATACTAAAT ACCAGGTATG GAGAAGCACT GTTTATGAAT 
TCCAAACTTA TCAGTGGAGT TACAGAATTT CTTAATACAG AAGGTGAACT TAAAGAGCTA 
AAGAATTTCA TGAAGTCCTA TGATGGGGTA GCCTCTGCTT CTTTCTCACG AGCTGTGGAA 
ACTGTGGAAG CCAATGTGCG CTGGAAAAGG TTTTACCAAG ATGAGCTTTT CCAGTGGCTG 
GGAAAAGCCA TGAGACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666353.2
CDS	880..4080
Translation	MALDGERGEQEEEKKKKKKKKKRKKKEEEGAEKSSSPFAATMGEDDAALRASGRGLSDPWADSVGVRPRTTERHIAVHKRLVLAFAVSIVALLAVTMLAVLLSLRFDECGASAAMPGTDGGLGGFPERDSNSSFPGSARRNHHAGGESSQRESGEVGTPGTPSAQPPSEEEREQWQPWTQLRLSGHLKPLHYNLMLTAFMENFTFSGEVNVEIACRNATRYVVLHASRVAVEKVQVAEDRAFGAVPVAGFFLYPQTQVLVVVLNRTLDAQRHYNLKIIYNALIENELLGFFRSSYVIHGERRFLGVTQFSPTHARKAFPCFDEPIYKATFKISIKHQATYLSLSNMPVETSVFEEDGWVTDHFSQTPLMSTYYLAWAICNFTYRETTTKSGVVVRLYARPDAIRRGSGDYALHITKRLIEFYEDYFKVPYSLPKLDLLAVPKHPYAAMENWGLSIFVEQRILLDPSVSSISYLLDVTMVIVHEICHQWFGDLVTPVWWEDVWLKEGFAHYFEFVGTDYLYPAWNMEKQRFLTDVLHEVMLLDGLASSHPVSQEVLRATDIDRVFDWIAYKKGAALIRMLANFMGHSVFQRGLQDYLTIHKYGNAARNDLWNTLSEALRRNGKYVNIQEVMDQWTLQMGYPVITILGNTTAENRILITQQHFIYDIGAKTKALQLQNSSYLWQIPLTIVVGNRSHVSSEAIIWVSNKSEHHRIAYLDRGSWILGNINQTGYFRVNYDLRNWRLLIDQLIRNHEVLSVSNRAALIDDAFSLARAGYLPQNIPLEIIRYLSEEKDFLPWHAASRALYPLDKLLDRMENYNIFNEYILKQVATTYIKLGWPRNNFNGSLVQASYQHEELRREVIMLACSFGNKHCHQQASTLISDWISSNRNRIPLNVRDIVYCTGVSLLDEDVWEFIWMKFHSTTAVSEKKILLEALTCSDDRNLLSRLLNLSLNSEVVLDQDAIDVIIHVARNPHGRDLAWKFFRDKWKILNTRYGEALFMNSKLISGVTEFLNTEGELKELKNFMKSYDGVASASFSRAVETVEANVRWKRFYQDELFQWLGKAMRH

Target ORF information:

RefSeq Version	NM_146241.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus TRH-degrading enzyme (Trhde), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_146241.3

ORF Insert Sequence:

ATGGCCCTGG ACGGCGAGAG GGGGGAGCAA GAGGAGGAGA AGAAAAAGAA GAAGAAGAAA 
AAGAAGAGGA AGAAGAAGGA GGAGGAGGGG GCAGAGAAGA GCAGTTCACC CTTTGCGGCC 
ACCATGGGAG AAGACGACGC CGCGCTCCGG GCAAGCGGCA GGGGGCTCTC GGACCCGTGG 
GCTGACTCGG TGGGAGTGAG ACCCCGCACC ACGGAGCGCC ACATCGCAGT GCACAAGAGG 
CTTGTGCTGG CCTTTGCCGT GTCCATCGTG GCACTGCTGG CTGTCACCAT GCTGGCCGTC 
CTGCTCAGCT TGCGATTCGA CGAGTGTGGA GCGAGCGCGG CGATGCCTGG CACCGACGGT 
GGCCTCGGGG GTTTCCCCGA GCGCGACAGC AACAGCAGCT TTCCAGGTTC TGCCCGGCGC 
AACCACCACG CGGGTGGGGA ATCCTCTCAG CGTGAGAGCG GCGAGGTGGG CACCCCGGGG 
ACCCCGTCTG CCCAGCCGCC GTCGGAGGAA GAGCGGGAGC AGTGGCAGCC CTGGACACAG 
CTGCGCCTAT CCGGCCACCT CAAGCCGCTG CACTACAATT TGATGCTCAC CGCTTTCATG 
GAGAACTTCA CCTTCTCTGG GGAGGTCAAC GTGGAGATCG CGTGCCGGAA CGCCACCCGC 
TACGTGGTAC TGCACGCCTC CCGAGTGGCG GTGGAGAAGG TGCAAGTAGC TGAAGACAGG 
GCGTTCGGGG CTGTCCCGGT TGCAGGCTTT TTCCTCTACC CACAAACGCA GGTCTTGGTG 
GTGGTATTGA ACAGAACCCT GGACGCGCAG AGGCATTACA ATCTGAAGAT TATCTACAAT 
GCCCTGATAG AGAACGAGCT TTTGGGCTTC TTCCGCAGCT CCTACGTGAT CCACGGGGAG 
AGAAGATTCC TTGGTGTTAC TCAGTTTTCA CCTACACATG CCAGGAAGGC GTTTCCGTGT 
TTCGATGAAC CAATCTACAA GGCCACTTTC AAAATCAGCA TCAAACATCA AGCAACCTAT 
TTGTCTCTAT CCAACATGCC AGTGGAAACG TCTGTGTTCG AGGAAGATGG ATGGGTGACA 
GATCACTTTT CACAGACCCC TCTCATGTCT ACATATTATT TAGCCTGGGC GATTTGCAAC 
TTCACATACA GAGAAACTAC CACCAAGAGT GGGGTTGTAG TCCGATTATA TGCGAGACCC 
GACGCTATCA GAAGAGGATC CGGGGACTAT GCTCTCCACA TTACAAAGAG ATTAATAGAA 
TTTTATGAAG ACTACTTTAA AGTGCCCTAT TCTTTGCCAA AACTAGATCT TTTAGCTGTG 
CCTAAGCATC CTTATGCTGC TATGGAGAAC TGGGGACTAA GTATTTTTGT GGAACAAAGA 
ATACTGCTGG ATCCCAGTGT GTCATCTATT TCTTATTTGC TGGATGTCAC CATGGTCATT 
GTTCATGAAA TATGTCACCA GTGGTTTGGT GACCTTGTGA CTCCAGTTTG GTGGGAAGAT 
GTGTGGTTGA AGGAAGGCTT TGCTCACTAC TTTGAATTTG TGGGTACAGA CTACCTCTAC 
CCTGCCTGGA ACATGGAGAA GCAGAGATTT CTGACAGATG TTCTGCATGA AGTGATGCTA 
CTTGATGGCT TGGCCAGTTC CCATCCAGTA TCACAGGAAG TGCTACGGGC AACAGATATT 
GACAGAGTGT TCGACTGGAT CGCATACAAA AAGGGTGCTG CTCTAATTAG AATGCTGGCT 
AATTTTATGG GTCATTCAGT TTTTCAGAGG GGATTGCAAG ATTATTTAAC CATTCACAAG 
TATGGCAATG CAGCCAGAAA TGATCTCTGG AATACATTAT CAGAGGCTTT AAGAAGGAAT 
GGAAAATACG TGAACATACA AGAAGTAATG GATCAGTGGA CACTGCAGAT GGGATATCCT 
GTTATCACTA TCCTGGGAAA CACGACGGCA GAAAATAGAA TACTTATCAC CCAACAGCAT 
TTCATTTATG ACATTGGTGC TAAAACTAAA GCACTTCAAC TTCAAAACAG CAGTTACCTG 
TGGCAGATTC CATTAACCAT TGTGGTAGGA AATAGAAGCC ATGTGTCTTC AGAAGCAATT 
ATTTGGGTGT CTAACAAATC AGAACATCAC AGAATAGCGT ACTTAGACAG AGGAAGCTGG 
ATCCTTGGGA ACATCAATCA AACCGGCTAC TTTAGAGTCA ACTATGACCT AAGGAACTGG 
AGATTACTAA TCGATCAGTT AATCAGGAAC CACGAGGTAC TTTCTGTCAG TAACCGCGCG 
GCTTTGATCG ATGATGCCTT CAGCCTGGCC AGGGCAGGCT ATTTGCCTCA GAATATCCCC 
CTAGAGATCA TCAGATACCT GTCTGAGGAA AAGGATTTTC TTCCTTGGCA CGCTGCCAGC 
CGAGCTCTTT ATCCTCTGGA TAAATTACTG GACCGCATGG AGAACTACAA TATCTTCAAT 
GAATATATTC TAAAACAAGT TGCAACAACA TACATCAAGC TTGGATGGCC AAGAAATAAC 
TTCAATGGAT CTCTCGTTCA AGCCTCCTAC CAACATGAGG AGCTACGGAG GGAAGTGATA 
ATGTTGGCAT GTAGTTTTGG GAACAAGCAC TGTCACCAGC AGGCATCAAC ACTTATTTCA 
GACTGGATTT CCAGCAACAG AAACAGAATA CCCCTGAATG TTAGAGACAT CGTCTACTGC 
ACAGGAGTGT CACTGCTGGA TGAGGATGTC TGGGAGTTTA TCTGGATGAA ATTCCACTCT 
ACCACTGCAG TTTCTGAGAA AAAGATACTG CTGGAGGCTC TAACTTGCAG CGATGACAGG 
AATTTATTAA GTAGGCTTCT AAATCTGTCT CTGAATTCTG AGGTGGTGTT GGATCAAGAT 
GCAATTGATG TAATTATCCA CGTAGCCAGA AATCCACATG GCCGAGACCT TGCCTGGAAG 
TTTTTCAGAG ACAAATGGAA AATACTAAAT ACCAGGTATG GAGAAGCACT GTTTATGAAT 
TCCAAACTTA TCAGTGGAGT TACAGAATTT CTTAATACAG AAGGTGAACT TAAAGAGCTA 
AAGAATTTCA TGAAGTCCTA TGATGGGGTA GCCTCTGCTT CTTTCTCACG AGCTGTGGAA 
ACTGTGGAAG CCAATGTGCG CTGGAAAAGG TTTTACCAAG ATGAGCTTTT CCAGTGGCTG 
GGAAAAGCCA TGAGACACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A mouse knockout library for secreted and transmembrane proteins.
Nature biotechnology28(7)749-55(2010 Jul)
Tang T,Li L,Tang J,Li Y,Lin WY,Martin F,Grant D,Solloway M,Parker L,Ye W,Forrest W,Ghilardi N,Oravecz T,Platt KA,Rice DS,Hansen GM,Abuin A,Eberhart DE,Godowski P,Holt KH,Peterson A,Zambrowicz BP,de Sauvage FJ

Comprehensive expression atlas of fibroblast growth factors and their receptors generated by a novel robotic in situ hybridization platform.
Developmental dynamics : an official publication of the American Association of Anatomists234(2)371-86(2005 Oct)
Yaylaoglu MB,Titmus A,Visel A,Alvarez-Bolado G,Thaller C,Eichele G

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Trhde cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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