Crag cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crag
Entrez Gene ID	31800
Full Name	Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein
Synonyms	CG12737,CJ,CRAG,Dmel\CG12737
General protein information	Preferred Names Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein Names CG12737 gene product from transcript CG12737-RD CG12737-PA CG12737-PB CG12737-PD Calmodulin-binding protein related to a Rab3 GDP-GTP exchange protein Crag-PA Crag-PB Crag-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 7F7-7F8

mRNA and Protein(s)

mRNA	Protein	Name
NM_080341.4	NP_525080.3	Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein, isoform A
NM_001169239.2	NP_001162710.2	Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein, isoform D
NM_167164.4	NP_727281.2	Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein, isoform B

Pathways from BioSystems

Homologies

Homo sapiens (human)	DENND4A	NP_001138295.1
Bos taurus (cattle)	DENND4A	NP_001192454.1
Gallus gallus (chicken)	DENND4A	XP_413911.4
Drosophila melanogaster (fruit fly)	Crag	NP_727281.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001102	XP_322065.3
Pan troglodytes (chimpanzee)	DENND4A	XP_523102.2
	DENND4A	XP_001109950.1
Rattus norvegicus (Norway rat)	Dennd4a	XP_006243364.1
Mus musculus (house mouse)	Dennd4a	NP_001156389.1
Canis lupus familiaris (dog)	DENND4A	XP_005638584.1
Danio rerio (zebrafish)	dennd4a	NP_001074458.1
Xenopus tropicalis (tropical clawed frog)	dennd4a	XP_004912733.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Upon light stimulation, Crag is required for trafficking of Rh from the trans-Golgi network to rhabdomere membranes via a Rab11-dependent vesicular transport.
Title: Crag is a GEF for Rab11 required for rhodopsin trafficking and maintenance of adult photoreceptor cells.

The loss of Crag leads to the anomalous accumulation of basement membrane components on both sides of epithelial cells without directly affecting the distribution of apical or basolateral membrane proteins.
Title: Crag regulates epithelial architecture and polarized deposition of basement membrane proteins in Drosophila.

The following Crag gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crag cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa01140	NM_001169239.2 Latest version!	Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa01140	NM_080341.4 Latest version!	Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OFa01141	NM_167164.4 Latest version!	Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crag ( NM_080341.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_525080.3 Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa01140
Clone ID Related Accession (Same CDS sequence)	NM_080341.4 , NM_001169239.2
Accession Version	NM_080341.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_080341.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAA AGCGCATCGC CGACTACTTT GTGGTGGCCG GAATGCCGGA GAATCCGCAG 
CTGCTGCAGG AGAACAGCTT CAATGACTCG GGAAGATTGA GAGCGGCCAC CACCATCGAA 
CCGATCACGG ACATCGGCGT CTATTTCCCG CTGCTGGGCG AGGAGGTGCC CGAGGGATAC 
GAGATCCTCT CGCACACGCC CACGGGTCTA CAGGCGAACC TCAATCACGG ATCGGTCCGC 
ACCACCGACT GCTACATCTA CTTCCGGCGT GGCAAGGATC GCCCGCCGCT GGTGGACATA 
GGAGTCCTGT ACGATGGCCA CGAGCGGATC ATGTCGGATG CGGAAATCGT TGCGGAGACG 
CCTGGCGGCA GGGTGGCCAA TGTGAACAAC TCGTCCGCGA AGACGTTCCT CACGTACCGA 
CGCGCCCGAG CGGACATGCC CTGCAACGAG CTGGTGGTCA CCGAACTGTG CGTCATTGTC 
CAGAGCAAGG GTGAGCGGGC TCCGCACGCC TTTTGCCTAA TCTACAAGAC CCTGAACAAG 
GGCTATGTTG GCAGCGACGT CTACCTGTGC TACAAGAAGT CCATGTACCG GCCTAAGCAC 
ATCAGCTACA AGCCGGAGAT CCTGCTGCGC TACCCCACCT TCGACCACAC CGACTTCCCG 
CTGAATCTGT GCCCGAGCGT GCCGCTCTTC TGCCTGCCAA TGGGCGCCTC CCTGGAGGCC 
TGGCCACACG TCAACGGCAC GGAGAAGCGG AAACCCATCA GTCCGGTGTT CAGCACTTTT 
GTGCTCACCG TGAGCGATGG CACCTACAAG GTTTACGGAT CGGCGTTAAC TTTCTACGAG 
GATTACGATG AGTCAAAGTT GTCGGCTGAG CAGAAGGAGC TGCTCGGTTG GGACGAGGAG 
TTCGGCGCCC AGCACTCGCT GCACATGATC AAGGCTATAT GTCTGCTGTC GCATCATCCC 
TTTGGCGACA CCTTCGACAA ATGGCTGAAG TACCTTCATC GAATGGTTCT GTATGACGTT 
AATATACCCA TTCCCGTGGA GCGCTACATC ACCCAGCTGC TGGACGAAGT ACCGTTTCCG 
GCGCCCAGCA TTCACCTGCA GCTGTCCAGC GAGTCCAACG ATCGCATACT GCTCACACAG 
CCGGAAGATT CCCCGCTGCC GCGAAGTGGC GCCGGTTTCC ACATACTGCT CCAGAACCTG 
GGCACCGACA ACTGTCTGCA TGTGCTGCTG CTGGCCCTCA CCGAACAGAA GATACTCATC 
CACTCACTCA GACCTGCCAC GTTGACTGCT GTGGCCGAAG CCATTGTCTC CCTGCTGTTT 
CCGTTCAAGT GGCAGTGTCC GTACATCCCA CTTTGTCCGC TGGGCTTGGC GGAGGTGCTC 
CATGCCCCAT TGCCTTATCT CATTGGCGTG GACTCGCGCT TCTTCGATTT GTACGAGCCG 
CCCACGGATG TCACCTGCAT CGATTTGGAC ACCAACAACA TTAGCCTATG TGAGTCGCAG 
CGTCACTTGT CACCCAAGCT GCTGCCGAAA CGGGCAGCCC GCCTACTTCG CCAGACGCTC 
ACCGAACTGG AGAATGCCAA GCCCATTAGC TATGACTCCA CAAACAGTTT GGATCGCGAT 
ATTCGAAAGC GGAAACGAGA ACTGGTGCTG GAGCAGCGCA TCCAGGAGGC CTTTCTGCTC 
TTCATGGCCA GCATTCTGCG CGGCTACAGA GACTTTCTGG TGCCCATATC CAAGGCACCA 
TCGGTGGGAG CCACCGATCC AAGTGCCCTC TTCCAGCTGA AGGCTTTTTT GCGATCGCGT 
GATAAGTCGC ACCAGAAGTT CTTCGAGCTG ATGATGAAGA CCCAGATGTT CATCCGTTTC 
ATTGAGGAGC GTTCCTTTGT CTCCGATGGC GATCATGGTC TCAGCTTCTT CGACGAGTGT 
GCGGAGAAGG TGGGCAACTA CGATGAAACG CCCGCCCAGC TGCATTTGGT GGACTGGGAC 
ACGGGGCAGA ACAGCGAGCG CACCAAGTAC ATCTTTCCGC CGGACAGCGT AACTCCGGCG 
GGATCTCAGC CTGGCGGCGG AGGAGGAGGA TTAGCCTACA ACTACGAGAA CTTTACGCTG 
CAACCGGAAC TGTTGCAGAG CACGAAGAAG ACGGCTTTAT CGAAATTTCT GCAGCTGCAG 
CTGAACGCTT CCCTCTCGCC AGGATCACCC ATTGCCCGGC GAACCAAACA CGAGATTAAG 
CTGTCCCAGA AGATGGCCAG CCGTTGTCAG CAGCATCCGG AGGCGTGGTC CAAATACTTG 
TTGGCCACCT GTTACTCGCT GTACTTCCTC ATCCTGCCAT CCATGGTCTT GGATCCGCGG 
CACGCCGGCA AGGAGCCAGA GATACTGCGC GCCGCCTACG ATGTCCTCGT GCGAGCCAGT 
CGTCTAAAGA TCACCTGTGA CGAGTTCTGC TACAGGATCA TGATGCAGCT ATGCGGTATT 
CACAATCTGC CTGTGCTGGC CGTCCGTCTG CACTACCTGA TGAAACGCTC TGGCGTGCAG 
GCCAATGCGC TCACCTATGG CTTCTACAAC CGCTGCGTCC TTGAGTCGCA GTGGCCCCAG 
GACAGTACCA CGATCAGCCA GATCCGCTGG AATCGCATTA AGAACGTGGT GTTGGGAGCG 
GCGCAGTTCC GCAAGGCTGG CAAACAAAGG GCCGCCTCCA AGGTGAACAA GTCGCTCAGT 
GCGTCCCAGG ACCAGAACCT CAGCACCCTG GAAACGGTGG ACGGACAGTC GCGCACCAGT 
TTGGCTTCCT CCTCCGGCGA TGGCGGTGGC CATGGACTGC TCGATTTTGC CGCCTTCGAT 
CGGCTCAGGA ACAAGTTGGG CAGCATTGTG CGGCAGACGG TGACGGGCGG CAATAACGAG 
ACAATGGGCG ATGCGGTCAA CAACACTGGG TTGCTCATAC CCGGCGAACT CAGCGCCCCG 
AATACGCCGA CCTATGGCGG TGACACCGAA ATCCTGGCCA AGGCACTTCA GCAACAGCAG 
CCACGCAAAC AGATCATGAG CATTGGCGGC GGCGATGACG ATGATGAGGA TGAGGACGAA 
GATGAATACA CGGCCGGATC GCCGAGCACG CCGCAAAAGC AACTGGAAGC GGGCGCCGAT 
GACCTGGAAT ACGCCGGTGG AGGAGACTAC GAGGCGGACG AGGAGGACGA AGATGAAGTC 
GACGAGCATG TGGCCGCACA GCGGGCACGT CAGCGAGTGC AGAGTCCAAC CAAAATCTCG 
CCACGCACGC CCGTGACCCA AAACGATCCA CTGGGTGCTC TCAACGAGGA GGAGTCCGCC 
GCCAGTGCCA CGCCCACGCA GGAGACGCAG CAGGAGCAGC AGCACTCACA GTCGCAGATC 
GACAGCAGCA TATACAGCGA CAAGCCGATC CTGTTCCGGG GCCAGAGGAG CGCCACTTTC 
GACGAGTCGA CGCAGATCGG GAAGAGTATG CACCGTTCGG AAACGATGCC GGTGGCCAGC 
AGCGGTGTGA CCAACAGCCT GGCCAACATC GGATCCTCGC TGAAGTTCAC GTTCGGACGT 
TATTCACCCG CACGATTGTC CCTTAAGAAA GACTTGAAAT TGCCCGCCAA TATTATAGAA 
AATATATCGA GCATAAGTCC CAGCTTGACG GGCAAGAAGT CGAACGAGTT GATCCAGGGC 
AGTCTGAGCA GCATCAAATC GGCGGCCAAT TCGCTGACCA AAAAGTTCGA CGAGATCAAA 
GGCGTGATAT CGGCCAATTC AACGCCGACG AAAACGAACA ATGGACACCA TCCGCATGGC 
CTGCATCATG GTCACCATCA TCCGCACCAC CACCATCATC ATCATTCGCA GCACGGCAAT 
GCGGAGCAGG AGGAGCACGA TGCCGCCGTC CACGAGGAGG GCAAGCTGCG TCGCGTCTCA 
AGCGACCTGG ATCCATGGGG CAGGCTCAGC GAGTCGCGTA AATCCAGCTA CAATAACCTC 
GTGCCGCTCG GCGAGAACAG CTCCACCGGC GCCCTTCACA TGCACGCCTT TCCCGCTGTG 
CCCGACAACC TCTATAGTCT AACAAGCGAG AACGCCGCGG ATCGTGACTG CGATGTGCTG 
ATCCAGCTGA CGACCTGCTC GCAGTGCCAC AATTGCTCGG TGCTCGTCTA CGACGAGGAG 
ATCATGAGCG GCTGGACGGC GGAGGACTCT AACCTGAACA CCACCTGCCA TGCCTGCAAC 
AAGCTCACGG TGCCTTTCCT CAGCGTACAG ATCGAACGGC AGGTGGAGGA GTCGGAACAG 
TCGGATCCGC TGCAGGACGG CAAGGAGCAA ATAGCCAACG GCAATAGCCA CAAGACCAGC 
CTGACCGTGC CGTATCTAAA TCCGCTGGTG CTGCGCAAGG AGCTGGAGAA CATACTCACG 
CAGGAGGGCG ACATTGCGCT GATAAAACCG GAATTTGTCG ATGAGCACCC GATCATCTAT 
TGGAACTTGC TCTGGCTGAT GGAGCGCATC GAGAGCAAGA CGCACCTGCC CGAGCTCTGT 
CTGCCCGTGC CGAGCGACAA GGAGCACATC GATCCGCTGA GCAAGGTGAA GACGGTGCAC 
ATCCAGTGCC TGTGGGACAA TCTGAGCCTG CATACGGAGG CCAGTGGTCC ACCCATGTAC 
TTGCTGTACA GGGAGACCCA GCCGACGAGT CCGCTCATCA AGGCGCTGCT CACCGATCAG 
GCGCAGCTCA ACAAGAATGT CATTCAGCAA ATCATTTCGG CCATCCGGTG CAATGACTTC 
GCCACTCCTC TCAAGCGCTT GGCCAATGAG CGACACAAGC TGAAGAGCAA CGGCGTGGAG 
CGATCGCACT CGTTCTATCG CGACATACTC TTCCTGGCCC TGACCGCCAT CGGTAGGTCC 
AACGTGGACC TGGCCACATT CCATCGCGAG TATGCAGCCG TGTTCGACAA GCTGACGGAG 
CGGGAGTGCA ACATGTACTA TCGCAATCAG GATCTGCCGC CCTCGGCATC GACGATCTTC 
TGTCGTGCTT ACTTCCGCCC GCTTCTGCTG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_525080.3
CDS	578..5593
Translation	MEEKRIADYFVVAGMPENPQLLQENSFNDSGRLRAATTIEPITDIGVYFPLLGEEVPEGYEILSHTPTGLQANLNHGSVRTTDCYIYFRRGKDRPPLVDIGVLYDGHERIMSDAEIVAETPGGRVANVNNSSAKTFLTYRRARADMPCNELVVTELCVIVQSKGERAPHAFCLIYKTLNKGYVGSDVYLCYKKSMYRPKHISYKPEILLRYPTFDHTDFPLNLCPSVPLFCLPMGASLEAWPHVNGTEKRKPISPVFSTFVLTVSDGTYKVYGSALTFYEDYDESKLSAEQKELLGWDEEFGAQHSLHMIKAICLLSHHPFGDTFDKWLKYLHRMVLYDVNIPIPVERYITQLLDEVPFPAPSIHLQLSSESNDRILLTQPEDSPLPRSGAGFHILLQNLGTDNCLHVLLLALTEQKILIHSLRPATLTAVAEAIVSLLFPFKWQCPYIPLCPLGLAEVLHAPLPYLIGVDSRFFDLYEPPTDVTCIDLDTNNISLCESQRHLSPKLLPKRAARLLRQTLTELENAKPISYDSTNSLDRDIRKRKRELVLEQRIQEAFLLFMASILRGYRDFLVPISKAPSVGATDPSALFQLKAFLRSRDKSHQKFFELMMKTQMFIRFIEERSFVSDGDHGLSFFDECAEKVGNYDETPAQLHLVDWDTGQNSERTKYIFPPDSVTPAGSQPGGGGGGLAYNYENFTLQPELLQSTKKTALSKFLQLQLNASLSPGSPIARRTKHEIKLSQKMASRCQQHPEAWSKYLLATCYSLYFLILPSMVLDPRHAGKEPEILRAAYDVLVRASRLKITCDEFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWNRIKNVVLGAAQFRKAGKQRAASKVNKSLSASQDQNLSTLETVDGQSRTSLASSSGDGGGHGLLDFAAFDRLRNKLGSIVRQTVTGGNNETMGDAVNNTGLLIPGELSAPNTPTYGGDTEILAKALQQQQPRKQIMSIGGGDDDDEDEDEDEYTAGSPSTPQKQLEAGADDLEYAGGGDYEADEEDEDEVDEHVAAQRARQRVQSPTKISPRTPVTQNDPLGALNEEESAASATPTQETQQEQQHSQSQIDSSIYSDKPILFRGQRSATFDESTQIGKSMHRSETMPVASSGVTNSLANIGSSLKFTFGRYSPARLSLKKDLKLPANIIENISSISPSLTGKKSNELIQGSLSSIKSAANSLTKKFDEIKGVISANSTPTKTNNGHHPHGLHHGHHHPHHHHHHHSQHGNAEQEEHDAAVHEEGKLRRVSSDLDPWGRLSESRKSSYNNLVPLGENSSTGALHMHAFPAVPDNLYSLTSENAADRDCDVLIQLTTCSQCHNCSVLVYDEEIMSGWTAEDSNLNTTCHACNKLTVPFLSVQIERQVEESEQSDPLQDGKEQIANGNSHKTSLTVPYLNPLVLRKELENILTQEGDIALIKPEFVDEHPIIYWNLLWLMERIESKTHLPELCLPVPSDKEHIDPLSKVKTVHIQCLWDNLSLHTEASGPPMYLLYRETQPTSPLIKALLTDQAQLNKNVIQQIISAIRCNDFATPLKRLANERHKLKSNGVERSHSFYRDILFLALTAIGRSNVDLATFHREYAAVFDKLTERECNMYYRNQDLPPSASTIFCRAYFRPLLLP

Target ORF information:

RefSeq Version	NM_080341.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080341.4

ORF Insert Sequence:

ATGGAGGAAA AGCGCATCGC CGACTACTTT GTGGTGGCCG GAATGCCGGA GAATCCGCAG 
CTGCTGCAGG AGAACAGCTT CAATGACTCG GGAAGATTGA GAGCGGCCAC CACCATCGAA 
CCGATCACGG ACATCGGCGT CTATTTCCCG CTGCTGGGCG AGGAGGTGCC CGAGGGATAC 
GAGATCCTCT CGCACACGCC CACGGGTCTA CAGGCGAACC TCAATCACGG ATCGGTCCGC 
ACCACCGACT GCTACATCTA CTTCCGGCGT GGCAAGGATC GCCCGCCGCT GGTGGACATA 
GGAGTCCTGT ACGATGGCCA CGAGCGGATC ATGTCGGATG CGGAAATCGT TGCGGAGACG 
CCTGGCGGCA GGGTGGCCAA TGTGAACAAC TCGTCCGCGA AGACGTTCCT CACGTACCGA 
CGCGCCCGAG CGGACATGCC CTGCAACGAG CTGGTGGTCA CCGAACTGTG CGTCATTGTC 
CAGAGCAAGG GTGAGCGGGC TCCGCACGCC TTTTGCCTAA TCTACAAGAC CCTGAACAAG 
GGCTATGTTG GCAGCGACGT CTACCTGTGC TACAAGAAGT CCATGTACCG GCCTAAGCAC 
ATCAGCTACA AGCCGGAGAT CCTGCTGCGC TACCCCACCT TCGACCACAC CGACTTCCCG 
CTGAATCTGT GCCCGAGCGT GCCGCTCTTC TGCCTGCCAA TGGGCGCCTC CCTGGAGGCC 
TGGCCACACG TCAACGGCAC GGAGAAGCGG AAACCCATCA GTCCGGTGTT CAGCACTTTT 
GTGCTCACCG TGAGCGATGG CACCTACAAG GTTTACGGAT CGGCGTTAAC TTTCTACGAG 
GATTACGATG AGTCAAAGTT GTCGGCTGAG CAGAAGGAGC TGCTCGGTTG GGACGAGGAG 
TTCGGCGCCC AGCACTCGCT GCACATGATC AAGGCTATAT GTCTGCTGTC GCATCATCCC 
TTTGGCGACA CCTTCGACAA ATGGCTGAAG TACCTTCATC GAATGGTTCT GTATGACGTT 
AATATACCCA TTCCCGTGGA GCGCTACATC ACCCAGCTGC TGGACGAAGT ACCGTTTCCG 
GCGCCCAGCA TTCACCTGCA GCTGTCCAGC GAGTCCAACG ATCGCATACT GCTCACACAG 
CCGGAAGATT CCCCGCTGCC GCGAAGTGGC GCCGGTTTCC ACATACTGCT CCAGAACCTG 
GGCACCGACA ACTGTCTGCA TGTGCTGCTG CTGGCCCTCA CCGAACAGAA GATACTCATC 
CACTCACTCA GACCTGCCAC GTTGACTGCT GTGGCCGAAG CCATTGTCTC CCTGCTGTTT 
CCGTTCAAGT GGCAGTGTCC GTACATCCCA CTTTGTCCGC TGGGCTTGGC GGAGGTGCTC 
CATGCCCCAT TGCCTTATCT CATTGGCGTG GACTCGCGCT TCTTCGATTT GTACGAGCCG 
CCCACGGATG TCACCTGCAT CGATTTGGAC ACCAACAACA TTAGCCTATG TGAGTCGCAG 
CGTCACTTGT CACCCAAGCT GCTGCCGAAA CGGGCAGCCC GCCTACTTCG CCAGACGCTC 
ACCGAACTGG AGAATGCCAA GCCCATTAGC TATGACTCCA CAAACAGTTT GGATCGCGAT 
ATTCGAAAGC GGAAACGAGA ACTGGTGCTG GAGCAGCGCA TCCAGGAGGC CTTTCTGCTC 
TTCATGGCCA GCATTCTGCG CGGCTACAGA GACTTTCTGG TGCCCATATC CAAGGCACCA 
TCGGTGGGAG CCACCGATCC AAGTGCCCTC TTCCAGCTGA AGGCTTTTTT GCGATCGCGT 
GATAAGTCGC ACCAGAAGTT CTTCGAGCTG ATGATGAAGA CCCAGATGTT CATCCGTTTC 
ATTGAGGAGC GTTCCTTTGT CTCCGATGGC GATCATGGTC TCAGCTTCTT CGACGAGTGT 
GCGGAGAAGG TGGGCAACTA CGATGAAACG CCCGCCCAGC TGCATTTGGT GGACTGGGAC 
ACGGGGCAGA ACAGCGAGCG CACCAAGTAC ATCTTTCCGC CGGACAGCGT AACTCCGGCG 
GGATCTCAGC CTGGCGGCGG AGGAGGAGGA TTAGCCTACA ACTACGAGAA CTTTACGCTG 
CAACCGGAAC TGTTGCAGAG CACGAAGAAG ACGGCTTTAT CGAAATTTCT GCAGCTGCAG 
CTGAACGCTT CCCTCTCGCC AGGATCACCC ATTGCCCGGC GAACCAAACA CGAGATTAAG 
CTGTCCCAGA AGATGGCCAG CCGTTGTCAG CAGCATCCGG AGGCGTGGTC CAAATACTTG 
TTGGCCACCT GTTACTCGCT GTACTTCCTC ATCCTGCCAT CCATGGTCTT GGATCCGCGG 
CACGCCGGCA AGGAGCCAGA GATACTGCGC GCCGCCTACG ATGTCCTCGT GCGAGCCAGT 
CGTCTAAAGA TCACCTGTGA CGAGTTCTGC TACAGGATCA TGATGCAGCT ATGCGGTATT 
CACAATCTGC CTGTGCTGGC CGTCCGTCTG CACTACCTGA TGAAACGCTC TGGCGTGCAG 
GCCAATGCGC TCACCTATGG CTTCTACAAC CGCTGCGTCC TTGAGTCGCA GTGGCCCCAG 
GACAGTACCA CGATCAGCCA GATCCGCTGG AATCGCATTA AGAACGTGGT GTTGGGAGCG 
GCGCAGTTCC GCAAGGCTGG CAAACAAAGG GCCGCCTCCA AGGTGAACAA GTCGCTCAGT 
GCGTCCCAGG ACCAGAACCT CAGCACCCTG GAAACGGTGG ACGGACAGTC GCGCACCAGT 
TTGGCTTCCT CCTCCGGCGA TGGCGGTGGC CATGGACTGC TCGATTTTGC CGCCTTCGAT 
CGGCTCAGGA ACAAGTTGGG CAGCATTGTG CGGCAGACGG TGACGGGCGG CAATAACGAG 
ACAATGGGCG ATGCGGTCAA CAACACTGGG TTGCTCATAC CCGGCGAACT CAGCGCCCCG 
AATACGCCGA CCTATGGCGG TGACACCGAA ATCCTGGCCA AGGCACTTCA GCAACAGCAG 
CCACGCAAAC AGATCATGAG CATTGGCGGC GGCGATGACG ATGATGAGGA TGAGGACGAA 
GATGAATACA CGGCCGGATC GCCGAGCACG CCGCAAAAGC AACTGGAAGC GGGCGCCGAT 
GACCTGGAAT ACGCCGGTGG AGGAGACTAC GAGGCGGACG AGGAGGACGA AGATGAAGTC 
GACGAGCATG TGGCCGCACA GCGGGCACGT CAGCGAGTGC AGAGTCCAAC CAAAATCTCG 
CCACGCACGC CCGTGACCCA AAACGATCCA CTGGGTGCTC TCAACGAGGA GGAGTCCGCC 
GCCAGTGCCA CGCCCACGCA GGAGACGCAG CAGGAGCAGC AGCACTCACA GTCGCAGATC 
GACAGCAGCA TATACAGCGA CAAGCCGATC CTGTTCCGGG GCCAGAGGAG CGCCACTTTC 
GACGAGTCGA CGCAGATCGG GAAGAGTATG CACCGTTCGG AAACGATGCC GGTGGCCAGC 
AGCGGTGTGA CCAACAGCCT GGCCAACATC GGATCCTCGC TGAAGTTCAC GTTCGGACGT 
TATTCACCCG CACGATTGTC CCTTAAGAAA GACTTGAAAT TGCCCGCCAA TATTATAGAA 
AATATATCGA GCATAAGTCC CAGCTTGACG GGCAAGAAGT CGAACGAGTT GATCCAGGGC 
AGTCTGAGCA GCATCAAATC GGCGGCCAAT TCGCTGACCA AAAAGTTCGA CGAGATCAAA 
GGCGTGATAT CGGCCAATTC AACGCCGACG AAAACGAACA ATGGACACCA TCCGCATGGC 
CTGCATCATG GTCACCATCA TCCGCACCAC CACCATCATC ATCATTCGCA GCACGGCAAT 
GCGGAGCAGG AGGAGCACGA TGCCGCCGTC CACGAGGAGG GCAAGCTGCG TCGCGTCTCA 
AGCGACCTGG ATCCATGGGG CAGGCTCAGC GAGTCGCGTA AATCCAGCTA CAATAACCTC 
GTGCCGCTCG GCGAGAACAG CTCCACCGGC GCCCTTCACA TGCACGCCTT TCCCGCTGTG 
CCCGACAACC TCTATAGTCT AACAAGCGAG AACGCCGCGG ATCGTGACTG CGATGTGCTG 
ATCCAGCTGA CGACCTGCTC GCAGTGCCAC AATTGCTCGG TGCTCGTCTA CGACGAGGAG 
ATCATGAGCG GCTGGACGGC GGAGGACTCT AACCTGAACA CCACCTGCCA TGCCTGCAAC 
AAGCTCACGG TGCCTTTCCT CAGCGTACAG ATCGAACGGC AGGTGGAGGA GTCGGAACAG 
TCGGATCCGC TGCAGGACGG CAAGGAGCAA ATAGCCAACG GCAATAGCCA CAAGACCAGC 
CTGACCGTGC CGTATCTAAA TCCGCTGGTG CTGCGCAAGG AGCTGGAGAA CATACTCACG 
CAGGAGGGCG ACATTGCGCT GATAAAACCG GAATTTGTCG ATGAGCACCC GATCATCTAT 
TGGAACTTGC TCTGGCTGAT GGAGCGCATC GAGAGCAAGA CGCACCTGCC CGAGCTCTGT 
CTGCCCGTGC CGAGCGACAA GGAGCACATC GATCCGCTGA GCAAGGTGAA GACGGTGCAC 
ATCCAGTGCC TGTGGGACAA TCTGAGCCTG CATACGGAGG CCAGTGGTCC ACCCATGTAC 
TTGCTGTACA GGGAGACCCA GCCGACGAGT CCGCTCATCA AGGCGCTGCT CACCGATCAG 
GCGCAGCTCA ACAAGAATGT CATTCAGCAA ATCATTTCGG CCATCCGGTG CAATGACTTC 
GCCACTCCTC TCAAGCGCTT GGCCAATGAG CGACACAAGC TGAAGAGCAA CGGCGTGGAG 
CGATCGCACT CGTTCTATCG CGACATACTC TTCCTGGCCC TGACCGCCAT CGGTAGGTCC 
AACGTGGACC TGGCCACATT CCATCGCGAG TATGCAGCCG TGTTCGACAA GCTGACGGAG 
CGGGAGTGCA ACATGTACTA TCGCAATCAG GATCTGCCGC CCTCGGCATC GACGATCTTC 
TGTCGTGCTT ACTTCCGCCC GCTTCTGCTG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crag ( NM_001169239.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001162710.2 Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant D, mRNA.

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CloneID	OFa01140
Clone ID Related Accession (Same CDS sequence)	NM_080341.4 , NM_001169239.2
Accession Version	NM_001169239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_001169239.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAA AGCGCATCGC CGACTACTTT GTGGTGGCCG GAATGCCGGA GAATCCGCAG 
CTGCTGCAGG AGAACAGCTT CAATGACTCG GGAAGATTGA GAGCGGCCAC CACCATCGAA 
CCGATCACGG ACATCGGCGT CTATTTCCCG CTGCTGGGCG AGGAGGTGCC CGAGGGATAC 
GAGATCCTCT CGCACACGCC CACGGGTCTA CAGGCGAACC TCAATCACGG ATCGGTCCGC 
ACCACCGACT GCTACATCTA CTTCCGGCGT GGCAAGGATC GCCCGCCGCT GGTGGACATA 
GGAGTCCTGT ACGATGGCCA CGAGCGGATC ATGTCGGATG CGGAAATCGT TGCGGAGACG 
CCTGGCGGCA GGGTGGCCAA TGTGAACAAC TCGTCCGCGA AGACGTTCCT CACGTACCGA 
CGCGCCCGAG CGGACATGCC CTGCAACGAG CTGGTGGTCA CCGAACTGTG CGTCATTGTC 
CAGAGCAAGG GTGAGCGGGC TCCGCACGCC TTTTGCCTAA TCTACAAGAC CCTGAACAAG 
GGCTATGTTG GCAGCGACGT CTACCTGTGC TACAAGAAGT CCATGTACCG GCCTAAGCAC 
ATCAGCTACA AGCCGGAGAT CCTGCTGCGC TACCCCACCT TCGACCACAC CGACTTCCCG 
CTGAATCTGT GCCCGAGCGT GCCGCTCTTC TGCCTGCCAA TGGGCGCCTC CCTGGAGGCC 
TGGCCACACG TCAACGGCAC GGAGAAGCGG AAACCCATCA GTCCGGTGTT CAGCACTTTT 
GTGCTCACCG TGAGCGATGG CACCTACAAG GTTTACGGAT CGGCGTTAAC TTTCTACGAG 
GATTACGATG AGTCAAAGTT GTCGGCTGAG CAGAAGGAGC TGCTCGGTTG GGACGAGGAG 
TTCGGCGCCC AGCACTCGCT GCACATGATC AAGGCTATAT GTCTGCTGTC GCATCATCCC 
TTTGGCGACA CCTTCGACAA ATGGCTGAAG TACCTTCATC GAATGGTTCT GTATGACGTT 
AATATACCCA TTCCCGTGGA GCGCTACATC ACCCAGCTGC TGGACGAAGT ACCGTTTCCG 
GCGCCCAGCA TTCACCTGCA GCTGTCCAGC GAGTCCAACG ATCGCATACT GCTCACACAG 
CCGGAAGATT CCCCGCTGCC GCGAAGTGGC GCCGGTTTCC ACATACTGCT CCAGAACCTG 
GGCACCGACA ACTGTCTGCA TGTGCTGCTG CTGGCCCTCA CCGAACAGAA GATACTCATC 
CACTCACTCA GACCTGCCAC GTTGACTGCT GTGGCCGAAG CCATTGTCTC CCTGCTGTTT 
CCGTTCAAGT GGCAGTGTCC GTACATCCCA CTTTGTCCGC TGGGCTTGGC GGAGGTGCTC 
CATGCCCCAT TGCCTTATCT CATTGGCGTG GACTCGCGCT TCTTCGATTT GTACGAGCCG 
CCCACGGATG TCACCTGCAT CGATTTGGAC ACCAACAACA TTAGCCTATG TGAGTCGCAG 
CGTCACTTGT CACCCAAGCT GCTGCCGAAA CGGGCAGCCC GCCTACTTCG CCAGACGCTC 
ACCGAACTGG AGAATGCCAA GCCCATTAGC TATGACTCCA CAAACAGTTT GGATCGCGAT 
ATTCGAAAGC GGAAACGAGA ACTGGTGCTG GAGCAGCGCA TCCAGGAGGC CTTTCTGCTC 
TTCATGGCCA GCATTCTGCG CGGCTACAGA GACTTTCTGG TGCCCATATC CAAGGCACCA 
TCGGTGGGAG CCACCGATCC AAGTGCCCTC TTCCAGCTGA AGGCTTTTTT GCGATCGCGT 
GATAAGTCGC ACCAGAAGTT CTTCGAGCTG ATGATGAAGA CCCAGATGTT CATCCGTTTC 
ATTGAGGAGC GTTCCTTTGT CTCCGATGGC GATCATGGTC TCAGCTTCTT CGACGAGTGT 
GCGGAGAAGG TGGGCAACTA CGATGAAACG CCCGCCCAGC TGCATTTGGT GGACTGGGAC 
ACGGGGCAGA ACAGCGAGCG CACCAAGTAC ATCTTTCCGC CGGACAGCGT AACTCCGGCG 
GGATCTCAGC CTGGCGGCGG AGGAGGAGGA TTAGCCTACA ACTACGAGAA CTTTACGCTG 
CAACCGGAAC TGTTGCAGAG CACGAAGAAG ACGGCTTTAT CGAAATTTCT GCAGCTGCAG 
CTGAACGCTT CCCTCTCGCC AGGATCACCC ATTGCCCGGC GAACCAAACA CGAGATTAAG 
CTGTCCCAGA AGATGGCCAG CCGTTGTCAG CAGCATCCGG AGGCGTGGTC CAAATACTTG 
TTGGCCACCT GTTACTCGCT GTACTTCCTC ATCCTGCCAT CCATGGTCTT GGATCCGCGG 
CACGCCGGCA AGGAGCCAGA GATACTGCGC GCCGCCTACG ATGTCCTCGT GCGAGCCAGT 
CGTCTAAAGA TCACCTGTGA CGAGTTCTGC TACAGGATCA TGATGCAGCT ATGCGGTATT 
CACAATCTGC CTGTGCTGGC CGTCCGTCTG CACTACCTGA TGAAACGCTC TGGCGTGCAG 
GCCAATGCGC TCACCTATGG CTTCTACAAC CGCTGCGTCC TTGAGTCGCA GTGGCCCCAG 
GACAGTACCA CGATCAGCCA GATCCGCTGG AATCGCATTA AGAACGTGGT GTTGGGAGCG 
GCGCAGTTCC GCAAGGCTGG CAAACAAAGG GCCGCCTCCA AGGTGAACAA GTCGCTCAGT 
GCGTCCCAGG ACCAGAACCT CAGCACCCTG GAAACGGTGG ACGGACAGTC GCGCACCAGT 
TTGGCTTCCT CCTCCGGCGA TGGCGGTGGC CATGGACTGC TCGATTTTGC CGCCTTCGAT 
CGGCTCAGGA ACAAGTTGGG CAGCATTGTG CGGCAGACGG TGACGGGCGG CAATAACGAG 
ACAATGGGCG ATGCGGTCAA CAACACTGGG TTGCTCATAC CCGGCGAACT CAGCGCCCCG 
AATACGCCGA CCTATGGCGG TGACACCGAA ATCCTGGCCA AGGCACTTCA GCAACAGCAG 
CCACGCAAAC AGATCATGAG CATTGGCGGC GGCGATGACG ATGATGAGGA TGAGGACGAA 
GATGAATACA CGGCCGGATC GCCGAGCACG CCGCAAAAGC AACTGGAAGC GGGCGCCGAT 
GACCTGGAAT ACGCCGGTGG AGGAGACTAC GAGGCGGACG AGGAGGACGA AGATGAAGTC 
GACGAGCATG TGGCCGCACA GCGGGCACGT CAGCGAGTGC AGAGTCCAAC CAAAATCTCG 
CCACGCACGC CCGTGACCCA AAACGATCCA CTGGGTGCTC TCAACGAGGA GGAGTCCGCC 
GCCAGTGCCA CGCCCACGCA GGAGACGCAG CAGGAGCAGC AGCACTCACA GTCGCAGATC 
GACAGCAGCA TATACAGCGA CAAGCCGATC CTGTTCCGGG GCCAGAGGAG CGCCACTTTC 
GACGAGTCGA CGCAGATCGG GAAGAGTATG CACCGTTCGG AAACGATGCC GGTGGCCAGC 
AGCGGTGTGA CCAACAGCCT GGCCAACATC GGATCCTCGC TGAAGTTCAC GTTCGGACGT 
TATTCACCCG CACGATTGTC CCTTAAGAAA GACTTGAAAT TGCCCGCCAA TATTATAGAA 
AATATATCGA GCATAAGTCC CAGCTTGACG GGCAAGAAGT CGAACGAGTT GATCCAGGGC 
AGTCTGAGCA GCATCAAATC GGCGGCCAAT TCGCTGACCA AAAAGTTCGA CGAGATCAAA 
GGCGTGATAT CGGCCAATTC AACGCCGACG AAAACGAACA ATGGACACCA TCCGCATGGC 
CTGCATCATG GTCACCATCA TCCGCACCAC CACCATCATC ATCATTCGCA GCACGGCAAT 
GCGGAGCAGG AGGAGCACGA TGCCGCCGTC CACGAGGAGG GCAAGCTGCG TCGCGTCTCA 
AGCGACCTGG ATCCATGGGG CAGGCTCAGC GAGTCGCGTA AATCCAGCTA CAATAACCTC 
GTGCCGCTCG GCGAGAACAG CTCCACCGGC GCCCTTCACA TGCACGCCTT TCCCGCTGTG 
CCCGACAACC TCTATAGTCT AACAAGCGAG AACGCCGCGG ATCGTGACTG CGATGTGCTG 
ATCCAGCTGA CGACCTGCTC GCAGTGCCAC AATTGCTCGG TGCTCGTCTA CGACGAGGAG 
ATCATGAGCG GCTGGACGGC GGAGGACTCT AACCTGAACA CCACCTGCCA TGCCTGCAAC 
AAGCTCACGG TGCCTTTCCT CAGCGTACAG ATCGAACGGC AGGTGGAGGA GTCGGAACAG 
TCGGATCCGC TGCAGGACGG CAAGGAGCAA ATAGCCAACG GCAATAGCCA CAAGACCAGC 
CTGACCGTGC CGTATCTAAA TCCGCTGGTG CTGCGCAAGG AGCTGGAGAA CATACTCACG 
CAGGAGGGCG ACATTGCGCT GATAAAACCG GAATTTGTCG ATGAGCACCC GATCATCTAT 
TGGAACTTGC TCTGGCTGAT GGAGCGCATC GAGAGCAAGA CGCACCTGCC CGAGCTCTGT 
CTGCCCGTGC CGAGCGACAA GGAGCACATC GATCCGCTGA GCAAGGTGAA GACGGTGCAC 
ATCCAGTGCC TGTGGGACAA TCTGAGCCTG CATACGGAGG CCAGTGGTCC ACCCATGTAC 
TTGCTGTACA GGGAGACCCA GCCGACGAGT CCGCTCATCA AGGCGCTGCT CACCGATCAG 
GCGCAGCTCA ACAAGAATGT CATTCAGCAA ATCATTTCGG CCATCCGGTG CAATGACTTC 
GCCACTCCTC TCAAGCGCTT GGCCAATGAG CGACACAAGC TGAAGAGCAA CGGCGTGGAG 
CGATCGCACT CGTTCTATCG CGACATACTC TTCCTGGCCC TGACCGCCAT CGGTAGGTCC 
AACGTGGACC TGGCCACATT CCATCGCGAG TATGCAGCCG TGTTCGACAA GCTGACGGAG 
CGGGAGTGCA ACATGTACTA TCGCAATCAG GATCTGCCGC CCTCGGCATC GACGATCTTC 
TGTCGTGCTT ACTTCCGCCC GCTTCTGCTG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001162710.2
CDS	578..5593
Translation	MEEKRIADYFVVAGMPENPQLLQENSFNDSGRLRAATTIEPITDIGVYFPLLGEEVPEGYEILSHTPTGLQANLNHGSVRTTDCYIYFRRGKDRPPLVDIGVLYDGHERIMSDAEIVAETPGGRVANVNNSSAKTFLTYRRARADMPCNELVVTELCVIVQSKGERAPHAFCLIYKTLNKGYVGSDVYLCYKKSMYRPKHISYKPEILLRYPTFDHTDFPLNLCPSVPLFCLPMGASLEAWPHVNGTEKRKPISPVFSTFVLTVSDGTYKVYGSALTFYEDYDESKLSAEQKELLGWDEEFGAQHSLHMIKAICLLSHHPFGDTFDKWLKYLHRMVLYDVNIPIPVERYITQLLDEVPFPAPSIHLQLSSESNDRILLTQPEDSPLPRSGAGFHILLQNLGTDNCLHVLLLALTEQKILIHSLRPATLTAVAEAIVSLLFPFKWQCPYIPLCPLGLAEVLHAPLPYLIGVDSRFFDLYEPPTDVTCIDLDTNNISLCESQRHLSPKLLPKRAARLLRQTLTELENAKPISYDSTNSLDRDIRKRKRELVLEQRIQEAFLLFMASILRGYRDFLVPISKAPSVGATDPSALFQLKAFLRSRDKSHQKFFELMMKTQMFIRFIEERSFVSDGDHGLSFFDECAEKVGNYDETPAQLHLVDWDTGQNSERTKYIFPPDSVTPAGSQPGGGGGGLAYNYENFTLQPELLQSTKKTALSKFLQLQLNASLSPGSPIARRTKHEIKLSQKMASRCQQHPEAWSKYLLATCYSLYFLILPSMVLDPRHAGKEPEILRAAYDVLVRASRLKITCDEFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWNRIKNVVLGAAQFRKAGKQRAASKVNKSLSASQDQNLSTLETVDGQSRTSLASSSGDGGGHGLLDFAAFDRLRNKLGSIVRQTVTGGNNETMGDAVNNTGLLIPGELSAPNTPTYGGDTEILAKALQQQQPRKQIMSIGGGDDDDEDEDEDEYTAGSPSTPQKQLEAGADDLEYAGGGDYEADEEDEDEVDEHVAAQRARQRVQSPTKISPRTPVTQNDPLGALNEEESAASATPTQETQQEQQHSQSQIDSSIYSDKPILFRGQRSATFDESTQIGKSMHRSETMPVASSGVTNSLANIGSSLKFTFGRYSPARLSLKKDLKLPANIIENISSISPSLTGKKSNELIQGSLSSIKSAANSLTKKFDEIKGVISANSTPTKTNNGHHPHGLHHGHHHPHHHHHHHSQHGNAEQEEHDAAVHEEGKLRRVSSDLDPWGRLSESRKSSYNNLVPLGENSSTGALHMHAFPAVPDNLYSLTSENAADRDCDVLIQLTTCSQCHNCSVLVYDEEIMSGWTAEDSNLNTTCHACNKLTVPFLSVQIERQVEESEQSDPLQDGKEQIANGNSHKTSLTVPYLNPLVLRKELENILTQEGDIALIKPEFVDEHPIIYWNLLWLMERIESKTHLPELCLPVPSDKEHIDPLSKVKTVHIQCLWDNLSLHTEASGPPMYLLYRETQPTSPLIKALLTDQAQLNKNVIQQIISAIRCNDFATPLKRLANERHKLKSNGVERSHSFYRDILFLALTAIGRSNVDLATFHREYAAVFDKLTERECNMYYRNQDLPPSASTIFCRAYFRPLLLP

Target ORF information:

RefSeq Version	NM_001169239.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001169239.2

ORF Insert Sequence:

ATGGAGGAAA AGCGCATCGC CGACTACTTT GTGGTGGCCG GAATGCCGGA GAATCCGCAG 
CTGCTGCAGG AGAACAGCTT CAATGACTCG GGAAGATTGA GAGCGGCCAC CACCATCGAA 
CCGATCACGG ACATCGGCGT CTATTTCCCG CTGCTGGGCG AGGAGGTGCC CGAGGGATAC 
GAGATCCTCT CGCACACGCC CACGGGTCTA CAGGCGAACC TCAATCACGG ATCGGTCCGC 
ACCACCGACT GCTACATCTA CTTCCGGCGT GGCAAGGATC GCCCGCCGCT GGTGGACATA 
GGAGTCCTGT ACGATGGCCA CGAGCGGATC ATGTCGGATG CGGAAATCGT TGCGGAGACG 
CCTGGCGGCA GGGTGGCCAA TGTGAACAAC TCGTCCGCGA AGACGTTCCT CACGTACCGA 
CGCGCCCGAG CGGACATGCC CTGCAACGAG CTGGTGGTCA CCGAACTGTG CGTCATTGTC 
CAGAGCAAGG GTGAGCGGGC TCCGCACGCC TTTTGCCTAA TCTACAAGAC CCTGAACAAG 
GGCTATGTTG GCAGCGACGT CTACCTGTGC TACAAGAAGT CCATGTACCG GCCTAAGCAC 
ATCAGCTACA AGCCGGAGAT CCTGCTGCGC TACCCCACCT TCGACCACAC CGACTTCCCG 
CTGAATCTGT GCCCGAGCGT GCCGCTCTTC TGCCTGCCAA TGGGCGCCTC CCTGGAGGCC 
TGGCCACACG TCAACGGCAC GGAGAAGCGG AAACCCATCA GTCCGGTGTT CAGCACTTTT 
GTGCTCACCG TGAGCGATGG CACCTACAAG GTTTACGGAT CGGCGTTAAC TTTCTACGAG 
GATTACGATG AGTCAAAGTT GTCGGCTGAG CAGAAGGAGC TGCTCGGTTG GGACGAGGAG 
TTCGGCGCCC AGCACTCGCT GCACATGATC AAGGCTATAT GTCTGCTGTC GCATCATCCC 
TTTGGCGACA CCTTCGACAA ATGGCTGAAG TACCTTCATC GAATGGTTCT GTATGACGTT 
AATATACCCA TTCCCGTGGA GCGCTACATC ACCCAGCTGC TGGACGAAGT ACCGTTTCCG 
GCGCCCAGCA TTCACCTGCA GCTGTCCAGC GAGTCCAACG ATCGCATACT GCTCACACAG 
CCGGAAGATT CCCCGCTGCC GCGAAGTGGC GCCGGTTTCC ACATACTGCT CCAGAACCTG 
GGCACCGACA ACTGTCTGCA TGTGCTGCTG CTGGCCCTCA CCGAACAGAA GATACTCATC 
CACTCACTCA GACCTGCCAC GTTGACTGCT GTGGCCGAAG CCATTGTCTC CCTGCTGTTT 
CCGTTCAAGT GGCAGTGTCC GTACATCCCA CTTTGTCCGC TGGGCTTGGC GGAGGTGCTC 
CATGCCCCAT TGCCTTATCT CATTGGCGTG GACTCGCGCT TCTTCGATTT GTACGAGCCG 
CCCACGGATG TCACCTGCAT CGATTTGGAC ACCAACAACA TTAGCCTATG TGAGTCGCAG 
CGTCACTTGT CACCCAAGCT GCTGCCGAAA CGGGCAGCCC GCCTACTTCG CCAGACGCTC 
ACCGAACTGG AGAATGCCAA GCCCATTAGC TATGACTCCA CAAACAGTTT GGATCGCGAT 
ATTCGAAAGC GGAAACGAGA ACTGGTGCTG GAGCAGCGCA TCCAGGAGGC CTTTCTGCTC 
TTCATGGCCA GCATTCTGCG CGGCTACAGA GACTTTCTGG TGCCCATATC CAAGGCACCA 
TCGGTGGGAG CCACCGATCC AAGTGCCCTC TTCCAGCTGA AGGCTTTTTT GCGATCGCGT 
GATAAGTCGC ACCAGAAGTT CTTCGAGCTG ATGATGAAGA CCCAGATGTT CATCCGTTTC 
ATTGAGGAGC GTTCCTTTGT CTCCGATGGC GATCATGGTC TCAGCTTCTT CGACGAGTGT 
GCGGAGAAGG TGGGCAACTA CGATGAAACG CCCGCCCAGC TGCATTTGGT GGACTGGGAC 
ACGGGGCAGA ACAGCGAGCG CACCAAGTAC ATCTTTCCGC CGGACAGCGT AACTCCGGCG 
GGATCTCAGC CTGGCGGCGG AGGAGGAGGA TTAGCCTACA ACTACGAGAA CTTTACGCTG 
CAACCGGAAC TGTTGCAGAG CACGAAGAAG ACGGCTTTAT CGAAATTTCT GCAGCTGCAG 
CTGAACGCTT CCCTCTCGCC AGGATCACCC ATTGCCCGGC GAACCAAACA CGAGATTAAG 
CTGTCCCAGA AGATGGCCAG CCGTTGTCAG CAGCATCCGG AGGCGTGGTC CAAATACTTG 
TTGGCCACCT GTTACTCGCT GTACTTCCTC ATCCTGCCAT CCATGGTCTT GGATCCGCGG 
CACGCCGGCA AGGAGCCAGA GATACTGCGC GCCGCCTACG ATGTCCTCGT GCGAGCCAGT 
CGTCTAAAGA TCACCTGTGA CGAGTTCTGC TACAGGATCA TGATGCAGCT ATGCGGTATT 
CACAATCTGC CTGTGCTGGC CGTCCGTCTG CACTACCTGA TGAAACGCTC TGGCGTGCAG 
GCCAATGCGC TCACCTATGG CTTCTACAAC CGCTGCGTCC TTGAGTCGCA GTGGCCCCAG 
GACAGTACCA CGATCAGCCA GATCCGCTGG AATCGCATTA AGAACGTGGT GTTGGGAGCG 
GCGCAGTTCC GCAAGGCTGG CAAACAAAGG GCCGCCTCCA AGGTGAACAA GTCGCTCAGT 
GCGTCCCAGG ACCAGAACCT CAGCACCCTG GAAACGGTGG ACGGACAGTC GCGCACCAGT 
TTGGCTTCCT CCTCCGGCGA TGGCGGTGGC CATGGACTGC TCGATTTTGC CGCCTTCGAT 
CGGCTCAGGA ACAAGTTGGG CAGCATTGTG CGGCAGACGG TGACGGGCGG CAATAACGAG 
ACAATGGGCG ATGCGGTCAA CAACACTGGG TTGCTCATAC CCGGCGAACT CAGCGCCCCG 
AATACGCCGA CCTATGGCGG TGACACCGAA ATCCTGGCCA AGGCACTTCA GCAACAGCAG 
CCACGCAAAC AGATCATGAG CATTGGCGGC GGCGATGACG ATGATGAGGA TGAGGACGAA 
GATGAATACA CGGCCGGATC GCCGAGCACG CCGCAAAAGC AACTGGAAGC GGGCGCCGAT 
GACCTGGAAT ACGCCGGTGG AGGAGACTAC GAGGCGGACG AGGAGGACGA AGATGAAGTC 
GACGAGCATG TGGCCGCACA GCGGGCACGT CAGCGAGTGC AGAGTCCAAC CAAAATCTCG 
CCACGCACGC CCGTGACCCA AAACGATCCA CTGGGTGCTC TCAACGAGGA GGAGTCCGCC 
GCCAGTGCCA CGCCCACGCA GGAGACGCAG CAGGAGCAGC AGCACTCACA GTCGCAGATC 
GACAGCAGCA TATACAGCGA CAAGCCGATC CTGTTCCGGG GCCAGAGGAG CGCCACTTTC 
GACGAGTCGA CGCAGATCGG GAAGAGTATG CACCGTTCGG AAACGATGCC GGTGGCCAGC 
AGCGGTGTGA CCAACAGCCT GGCCAACATC GGATCCTCGC TGAAGTTCAC GTTCGGACGT 
TATTCACCCG CACGATTGTC CCTTAAGAAA GACTTGAAAT TGCCCGCCAA TATTATAGAA 
AATATATCGA GCATAAGTCC CAGCTTGACG GGCAAGAAGT CGAACGAGTT GATCCAGGGC 
AGTCTGAGCA GCATCAAATC GGCGGCCAAT TCGCTGACCA AAAAGTTCGA CGAGATCAAA 
GGCGTGATAT CGGCCAATTC AACGCCGACG AAAACGAACA ATGGACACCA TCCGCATGGC 
CTGCATCATG GTCACCATCA TCCGCACCAC CACCATCATC ATCATTCGCA GCACGGCAAT 
GCGGAGCAGG AGGAGCACGA TGCCGCCGTC CACGAGGAGG GCAAGCTGCG TCGCGTCTCA 
AGCGACCTGG ATCCATGGGG CAGGCTCAGC GAGTCGCGTA AATCCAGCTA CAATAACCTC 
GTGCCGCTCG GCGAGAACAG CTCCACCGGC GCCCTTCACA TGCACGCCTT TCCCGCTGTG 
CCCGACAACC TCTATAGTCT AACAAGCGAG AACGCCGCGG ATCGTGACTG CGATGTGCTG 
ATCCAGCTGA CGACCTGCTC GCAGTGCCAC AATTGCTCGG TGCTCGTCTA CGACGAGGAG 
ATCATGAGCG GCTGGACGGC GGAGGACTCT AACCTGAACA CCACCTGCCA TGCCTGCAAC 
AAGCTCACGG TGCCTTTCCT CAGCGTACAG ATCGAACGGC AGGTGGAGGA GTCGGAACAG 
TCGGATCCGC TGCAGGACGG CAAGGAGCAA ATAGCCAACG GCAATAGCCA CAAGACCAGC 
CTGACCGTGC CGTATCTAAA TCCGCTGGTG CTGCGCAAGG AGCTGGAGAA CATACTCACG 
CAGGAGGGCG ACATTGCGCT GATAAAACCG GAATTTGTCG ATGAGCACCC GATCATCTAT 
TGGAACTTGC TCTGGCTGAT GGAGCGCATC GAGAGCAAGA CGCACCTGCC CGAGCTCTGT 
CTGCCCGTGC CGAGCGACAA GGAGCACATC GATCCGCTGA GCAAGGTGAA GACGGTGCAC 
ATCCAGTGCC TGTGGGACAA TCTGAGCCTG CATACGGAGG CCAGTGGTCC ACCCATGTAC 
TTGCTGTACA GGGAGACCCA GCCGACGAGT CCGCTCATCA AGGCGCTGCT CACCGATCAG 
GCGCAGCTCA ACAAGAATGT CATTCAGCAA ATCATTTCGG CCATCCGGTG CAATGACTTC 
GCCACTCCTC TCAAGCGCTT GGCCAATGAG CGACACAAGC TGAAGAGCAA CGGCGTGGAG 
CGATCGCACT CGTTCTATCG CGACATACTC TTCCTGGCCC TGACCGCCAT CGGTAGGTCC 
AACGTGGACC TGGCCACATT CCATCGCGAG TATGCAGCCG TGTTCGACAA GCTGACGGAG 
CGGGAGTGCA ACATGTACTA TCGCAATCAG GATCTGCCGC CCTCGGCATC GACGATCTTC 
TGTCGTGCTT ACTTCCGCCC GCTTCTGCTG CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crag ( NM_167164.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_727281.2 Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa01141
Clone ID Related Accession (Same CDS sequence)	NM_167164.4
Accession Version	NM_167164.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_167164.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGAGGAAA AGCGCATCGC CGACTACTTT GTGGTGGCCG GAATGCCGGA GAATCCGCAG 
CTGCTGCAGG AGAACAGCTT CAATGACTCG GGAAGATTGA GAGCGGCCAC CACCATCGAA 
CCGATCACGG ACATCGGCGT CTATTTCCCG CTGCTGGGCG AGGAGGTGCC CGAGGGATAC 
GAGATCCTCT CGCACACGCC CACGGGTCTA CAGGCGAACC TCAATCACGG ATCGGTCCGC 
ACCACCGACT GCTACATCTA CTTCCGGCGT GGCAAGGATC GCCCGCCGCT GGTGGACATA 
GGAGTCCTGT ACGATGGCCA CGAGCGGATC ATGTCGGATG CGGAAATCGT TGCGGAGACG 
CCTGGCGGCA GGGTGGCCAA TGTGAACAAC TCGTCCGCGA AGACGTTCCT CACGTACCGA 
CGCGCCCGAG CGGACATGCC CTGCAACGAG CTGGTGGTCA CCGAACTGTG CGTCATTGTC 
CAGAGCAAGG GTGAGCGGGC TCCGCACGCC TTTTGCCTAA TCTACAAGAC CCTGAACAAG 
GGCTATGTTG GCAGCGACGT CTACCTGTGC TACAAGAAGT CCATGTACCG GCCTAAGCAC 
ATCAGCTACA AGCCGGAGAT CCTGCTGCGC TACCCCACCT TCGACCACAC CGACTTCCCG 
CTGAATCTGT GCCCGAGCGT GCCGCTCTTC TGCCTGCCAA TGGGCGCCTC CCTGGAGGCC 
TGGCCACACG TCAACGGCAC GGAGAAGCGG AAACCCATCA GTCCGGTGTT CAGCACTTTT 
GTGCTCACCG TGAGCGATGG CACCTACAAG GTTTACGGAT CGGCGTTAAC TTTCTACGAG 
GATTACGATG AGTCAAAGTT GTCGGCTGAG CAGAAGGAGC TGCTCGGTTG GGACGAGGAG 
TTCGGCGCCC AGCACTCGCT GCACATGATC AAGGCTATAT GTCTGCTGTC GCATCATCCC 
TTTGGCGACA CCTTCGACAA ATGGCTGAAG TACCTTCATC GAATGGTTCT GTATGACGTT 
AATATACCCA TTCCCGTGGA GCGCTACATC ACCCAGCTGC TGGACGAAGT ACCGTTTCCG 
GCGCCCAGCA TTCACCTGCA GCTGTCCAGC GAGTCCAACG ATCGCATACT GCTCACACAG 
CCGGAAGATT CCCCGCTGCC GCGAAGTGGC GCCGGTTTCC ACATACTGCT CCAGAACCTG 
GGCACCGACA ACTGTCTGCA TGTGCTGCTG CTGGCCCTCA CCGAACAGAA GATACTCATC 
CACTCACTCA GACCTGCCAC GTTGACTGCT GTGGCCGAAG CCATTGTCTC CCTGCTGTTT 
CCGTTCAAGT GGCAGTGTCC GTACATCCCA CTTTGTCCGC TGGGCTTGGC GGAGGTGCTC 
CATGCCCCAT TGCCTTATCT CATTGGCGTG GACTCGCGCT TCTTCGATTT GTACGAGCCG 
CCCACGGATG TCACCTGCAT CGATTTGGAC ACCAACAACA TTAGCCTATG TGAGTCGCAG 
CGTCACTTGT CACCCAAGCT GCTGCCGAAA CGGGCAGCCC GCCTACTTCG CCAGACGCTC 
ACCGAACTGG AGAATGCCAA GCCCATTAGC TATGACTCCA CAAACAGTTT GGATCGCGAT 
ATTCGAAAGC GGAAACGAGA ACTGGTGCTG GAGCAGCGCA TCCAGGAGGC CTTTCTGCTC 
TTCATGGCCA GCATTCTGCG CGGCTACAGA GACTTTCTGG TGCCCATATC CAAGGCACCA 
TCGGTGGGAG CCACCGATCC AAGTGCCCTC TTCCAGCTGA AGGCTTTTTT GCGATCGCGT 
GATAAGTCGC ACCAGAAGTT CTTCGAGCTG ATGATGAAGA CCCAGATGTT CATCCGTTTC 
ATTGAGGAGC GTTCCTTTGT CTCCGATGGC GATCATGGTC TCAGCTTCTT CGACGAGTGT 
GCGGAGAAGG TGGGCAACTA CGATGAAACG CCCGCCCAGC TGCATTTGGT GGACTGGGAC 
ACGGGGCAGA ACAGCGAGCG CACCAAGTAC ATCTTTCCGC CGGACAGCGT AACTCCGGCG 
GGATCTCAGC CTGGCGGCGG AGGAGGAGGA TTAGCCTACA ACTACGAGAA CTTTACGCTG 
CAACCGGAAC TGTTGCAGAG CACGAAGAAG ACGGCTTTAT CGAAATTTCT GCAGCTGCAG 
CTGAACGCTT CCCTCTCGCC AGGATCACCC ATTGCCCGGC GAACCAAACA CGAGATTAAG 
CTGTCCCAGA AGATGGCCAG CCGTTGTCAG CAGCATCCGG AGGCGTGGTC CAAATACTTG 
TTGGCCACCT GTTACTCGCT GTACTTCCTC ATCCTGCCAT CCATGGTCTT GGATCCGCGG 
CACGCCGGCA AGGAGCCAGA GATACTGCGC GCCGCCTACG ATGTCCTCGT GCGAGCCAGT 
CGTCTAAAGA TCACCTGTGA CGAGTTCTGC TACAGGATCA TGATGCAGCT ATGCGGTATT 
CACAATCTGC CTGTGCTGGC CGTCCGTCTG CACTACCTGA TGAAACGCTC TGGCGTGCAG 
GCCAATGCGC TCACCTATGG CTTCTACAAC CGCTGCGTCC TTGAGTCGCA GTGGCCCCAG 
GACAGTACCA CGATCAGCCA GATCCGCTGG AATCGCATTA AGAACGTGGT GTTGGGAGCG 
GCGCAGTTCC GCAAGGCTGG CAAACAAAGG GCCGCCTCCA AGGTGAACAA GTCGCTCAGT 
GCGTCCCAGG ACCAGAACCT CAGCACCCTG GAAACGGTGG ACGGACAGTC GCGCACCAGT 
TTGGCTTCCT CCTCCGGCGA TGGCGGTGGC CATGGACTGC TCGATTTTGC CGCCTTCGAT 
CGGCTCAGGA ACAAGTTGGG CAGCATTGTG CGGCAGACGG TGACGGGCGG CAATAACGAG 
ACAATGGGCG ATGCGGTCAA CAACACTGGG TTGCTCATAC CCGGCGAACT CAGCGCCCCG 
AATACGCCGA CCTATGGCGG TGACACCGAA ATCCTGGCCA AGGCACTTCA GCAACAGCAG 
CCACGCAAAC AGATCATGAG CATTGGCGGC GGCGATGACG ATGATGAGGA TGAGGACGAA 
GATGAATACA CGGCCGGATC GCCGAGCACG CCGCAAAAGC AACTGGAAGC GGGCGCCGAT 
GACCTGGAAT ACGCCGGTGG AGGAGACTAC GAGGCGGACG AGGAGGACGA AGATGAAGTC 
GACGAGCATG TGGCCGCACA GCGGGCACGT CAGCGAGTGC AGAGTCCAAC CAAAATCTCG 
CCACGCACGC CCGTGACCCA AAACGATCCA CTGGGTGCTC TCAACGAGGA GGAGTCCGCC 
GCCAGTGCCA CGCCCACGCA GGAGACGCAG CAGGAGCAGC AGCACTCACA GTCGCAGATC 
GACAGCAGCA TATACAGCGA CAAGCCGATC CTGTTCCGGG GCCAGAGGAG CGCCACTTTC 
GACGAGTCGA CGCAGATCGG GAAGAGTATG CACCGTTCGG AAACGATGCC GGTGGCCAGC 
AGCGGTGTGA CCAACAGCCT GGCCAACATC GGATCCTCGC TGAAGTTCAC GTTCGGTCCC 
AGCTTGACGG GCAAGAAGTC GAACGAGTTG ATCCAGGGCA GTCTGAGCAG CATCAAATCG 
GCGGCCAATT CGCTGACCAA AAAGTTCGAC GAGATCAAAG GCGTGATATC GGCCAATTCA 
ACGCCGACGA AAACGAACAA TGGACACCAT CCGCATGGCC TGCATCATGG TCACCATCAT 
CCGCACCACC ACCATCATCA TCATTCGCAG CACGGCAATG CGGAGCAGGA GGAGCACGAT 
GCCGCCGTCC ACGAGGAGGG CAAGCTGCGT CGCGTCTCAA GCGACCTGGA TCCATGGGGC 
AGGCTCAGCG AGTCGCGTAA ATCCAGCTAC AATAACCTCG TGCCGCTCGG CGAGAACAGC 
TCCACCGGCG CCCTTCACAT GCACGCCTTT CCCGCTGTGC CCGACAACCT CTATAGTCTA 
ACAAGCGAGA ACGCCGCGGA TCGTGACTGC GATGTGCTGA TCCAGCTGAC GACCTGCTCG 
CAGTGCCACA ATTGCTCGGT GCTCGTCTAC GACGAGGAGA TCATGAGCGG CTGGACGGCG 
GAGGACTCTA ACCTGAACAC CACCTGCCAT GCCTGCAACA AGCTCACGGT GCCTTTCCTC 
AGCGTACAGA TCGAACGGCA GGTGGAGGAG TCGGAACAGT CGGATCCGCT GCAGGACGGC 
AAGGAGCAAA TAGCCAACGG CAATAGCCAC AAGACCAGCC TGACCGTGCC GTATCTAAAT 
CCGCTGGTGC TGCGCAAGGA GCTGGAGAAC ATACTCACGC AGGAGGGCGA CATTGCGCTG 
ATAAAACCGG AATTTGTCGA TGAGCACCCG ATCATCTATT GGAACTTGCT CTGGCTGATG 
GAGCGCATCG AGAGCAAGAC GCACCTGCCC GAGCTCTGTC TGCCCGTGCC GAGCGACAAG 
GAGCACATCG ATCCGCTGAG CAAGGTGAAG ACGGTGCACA TCCAGTGCCT GTGGGACAAT 
CTGAGCCTGC ATACGGAGGC CAGTGGTCCA CCCATGTACT TGCTGTACAG GGAGACCCAG 
CCGACGAGTC CGCTCATCAA GGCGCTGCTC ACCGATCAGG CGCAGCTCAA CAAGAATGTC 
ATTCAGCAAA TCATTTCGGC CATCCGGTGC AATGACTTCG CCACTCCTCT CAAGCGCTTG 
GCCAATGAGC GACACAAGCT GAAGAGCAAC GGCGTGGAGC GATCGCACTC GTTCTATCGC 
GACATACTCT TCCTGGCCCT GACCGCCATC GGTAGGTCCA ACGTGGACCT GGCCACATTC 
CATCGCGAGT ATGCAGCCGT GTTCGACAAG CTGACGGAGC GGGAGTGCAA CATGTACTAT 
CGCAATCAGG ATCTGCCGCC CTCGGCATCG ACGATCTTCT GTCGTGCTTA CTTCCGCCCG 
CTTCTGCTGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_727281.2
CDS	578..5512
Translation	MEEKRIADYFVVAGMPENPQLLQENSFNDSGRLRAATTIEPITDIGVYFPLLGEEVPEGYEILSHTPTGLQANLNHGSVRTTDCYIYFRRGKDRPPLVDIGVLYDGHERIMSDAEIVAETPGGRVANVNNSSAKTFLTYRRARADMPCNELVVTELCVIVQSKGERAPHAFCLIYKTLNKGYVGSDVYLCYKKSMYRPKHISYKPEILLRYPTFDHTDFPLNLCPSVPLFCLPMGASLEAWPHVNGTEKRKPISPVFSTFVLTVSDGTYKVYGSALTFYEDYDESKLSAEQKELLGWDEEFGAQHSLHMIKAICLLSHHPFGDTFDKWLKYLHRMVLYDVNIPIPVERYITQLLDEVPFPAPSIHLQLSSESNDRILLTQPEDSPLPRSGAGFHILLQNLGTDNCLHVLLLALTEQKILIHSLRPATLTAVAEAIVSLLFPFKWQCPYIPLCPLGLAEVLHAPLPYLIGVDSRFFDLYEPPTDVTCIDLDTNNISLCESQRHLSPKLLPKRAARLLRQTLTELENAKPISYDSTNSLDRDIRKRKRELVLEQRIQEAFLLFMASILRGYRDFLVPISKAPSVGATDPSALFQLKAFLRSRDKSHQKFFELMMKTQMFIRFIEERSFVSDGDHGLSFFDECAEKVGNYDETPAQLHLVDWDTGQNSERTKYIFPPDSVTPAGSQPGGGGGGLAYNYENFTLQPELLQSTKKTALSKFLQLQLNASLSPGSPIARRTKHEIKLSQKMASRCQQHPEAWSKYLLATCYSLYFLILPSMVLDPRHAGKEPEILRAAYDVLVRASRLKITCDEFCYRIMMQLCGIHNLPVLAVRLHYLMKRSGVQANALTYGFYNRCVLESQWPQDSTTISQIRWNRIKNVVLGAAQFRKAGKQRAASKVNKSLSASQDQNLSTLETVDGQSRTSLASSSGDGGGHGLLDFAAFDRLRNKLGSIVRQTVTGGNNETMGDAVNNTGLLIPGELSAPNTPTYGGDTEILAKALQQQQPRKQIMSIGGGDDDDEDEDEDEYTAGSPSTPQKQLEAGADDLEYAGGGDYEADEEDEDEVDEHVAAQRARQRVQSPTKISPRTPVTQNDPLGALNEEESAASATPTQETQQEQQHSQSQIDSSIYSDKPILFRGQRSATFDESTQIGKSMHRSETMPVASSGVTNSLANIGSSLKFTFGPSLTGKKSNELIQGSLSSIKSAANSLTKKFDEIKGVISANSTPTKTNNGHHPHGLHHGHHHPHHHHHHHSQHGNAEQEEHDAAVHEEGKLRRVSSDLDPWGRLSESRKSSYNNLVPLGENSSTGALHMHAFPAVPDNLYSLTSENAADRDCDVLIQLTTCSQCHNCSVLVYDEEIMSGWTAEDSNLNTTCHACNKLTVPFLSVQIERQVEESEQSDPLQDGKEQIANGNSHKTSLTVPYLNPLVLRKELENILTQEGDIALIKPEFVDEHPIIYWNLLWLMERIESKTHLPELCLPVPSDKEHIDPLSKVKTVHIQCLWDNLSLHTEASGPPMYLLYRETQPTSPLIKALLTDQAQLNKNVIQQIISAIRCNDFATPLKRLANERHKLKSNGVERSHSFYRDILFLALTAIGRSNVDLATFHREYAAVFDKLTERECNMYYRNQDLPPSASTIFCRAYFRPLLLP

Target ORF information:

RefSeq Version	NM_167164.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster Calmodulin-binding protein related to a Rab3 GDP/GTP exchange protein (Crag), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167164.4

ORF Insert Sequence:

ATGGAGGAAA AGCGCATCGC CGACTACTTT GTGGTGGCCG GAATGCCGGA GAATCCGCAG 
CTGCTGCAGG AGAACAGCTT CAATGACTCG GGAAGATTGA GAGCGGCCAC CACCATCGAA 
CCGATCACGG ACATCGGCGT CTATTTCCCG CTGCTGGGCG AGGAGGTGCC CGAGGGATAC 
GAGATCCTCT CGCACACGCC CACGGGTCTA CAGGCGAACC TCAATCACGG ATCGGTCCGC 
ACCACCGACT GCTACATCTA CTTCCGGCGT GGCAAGGATC GCCCGCCGCT GGTGGACATA 
GGAGTCCTGT ACGATGGCCA CGAGCGGATC ATGTCGGATG CGGAAATCGT TGCGGAGACG 
CCTGGCGGCA GGGTGGCCAA TGTGAACAAC TCGTCCGCGA AGACGTTCCT CACGTACCGA 
CGCGCCCGAG CGGACATGCC CTGCAACGAG CTGGTGGTCA CCGAACTGTG CGTCATTGTC 
CAGAGCAAGG GTGAGCGGGC TCCGCACGCC TTTTGCCTAA TCTACAAGAC CCTGAACAAG 
GGCTATGTTG GCAGCGACGT CTACCTGTGC TACAAGAAGT CCATGTACCG GCCTAAGCAC 
ATCAGCTACA AGCCGGAGAT CCTGCTGCGC TACCCCACCT TCGACCACAC CGACTTCCCG 
CTGAATCTGT GCCCGAGCGT GCCGCTCTTC TGCCTGCCAA TGGGCGCCTC CCTGGAGGCC 
TGGCCACACG TCAACGGCAC GGAGAAGCGG AAACCCATCA GTCCGGTGTT CAGCACTTTT 
GTGCTCACCG TGAGCGATGG CACCTACAAG GTTTACGGAT CGGCGTTAAC TTTCTACGAG 
GATTACGATG AGTCAAAGTT GTCGGCTGAG CAGAAGGAGC TGCTCGGTTG GGACGAGGAG 
TTCGGCGCCC AGCACTCGCT GCACATGATC AAGGCTATAT GTCTGCTGTC GCATCATCCC 
TTTGGCGACA CCTTCGACAA ATGGCTGAAG TACCTTCATC GAATGGTTCT GTATGACGTT 
AATATACCCA TTCCCGTGGA GCGCTACATC ACCCAGCTGC TGGACGAAGT ACCGTTTCCG 
GCGCCCAGCA TTCACCTGCA GCTGTCCAGC GAGTCCAACG ATCGCATACT GCTCACACAG 
CCGGAAGATT CCCCGCTGCC GCGAAGTGGC GCCGGTTTCC ACATACTGCT CCAGAACCTG 
GGCACCGACA ACTGTCTGCA TGTGCTGCTG CTGGCCCTCA CCGAACAGAA GATACTCATC 
CACTCACTCA GACCTGCCAC GTTGACTGCT GTGGCCGAAG CCATTGTCTC CCTGCTGTTT 
CCGTTCAAGT GGCAGTGTCC GTACATCCCA CTTTGTCCGC TGGGCTTGGC GGAGGTGCTC 
CATGCCCCAT TGCCTTATCT CATTGGCGTG GACTCGCGCT TCTTCGATTT GTACGAGCCG 
CCCACGGATG TCACCTGCAT CGATTTGGAC ACCAACAACA TTAGCCTATG TGAGTCGCAG 
CGTCACTTGT CACCCAAGCT GCTGCCGAAA CGGGCAGCCC GCCTACTTCG CCAGACGCTC 
ACCGAACTGG AGAATGCCAA GCCCATTAGC TATGACTCCA CAAACAGTTT GGATCGCGAT 
ATTCGAAAGC GGAAACGAGA ACTGGTGCTG GAGCAGCGCA TCCAGGAGGC CTTTCTGCTC 
TTCATGGCCA GCATTCTGCG CGGCTACAGA GACTTTCTGG TGCCCATATC CAAGGCACCA 
TCGGTGGGAG CCACCGATCC AAGTGCCCTC TTCCAGCTGA AGGCTTTTTT GCGATCGCGT 
GATAAGTCGC ACCAGAAGTT CTTCGAGCTG ATGATGAAGA CCCAGATGTT CATCCGTTTC 
ATTGAGGAGC GTTCCTTTGT CTCCGATGGC GATCATGGTC TCAGCTTCTT CGACGAGTGT 
GCGGAGAAGG TGGGCAACTA CGATGAAACG CCCGCCCAGC TGCATTTGGT GGACTGGGAC 
ACGGGGCAGA ACAGCGAGCG CACCAAGTAC ATCTTTCCGC CGGACAGCGT AACTCCGGCG 
GGATCTCAGC CTGGCGGCGG AGGAGGAGGA TTAGCCTACA ACTACGAGAA CTTTACGCTG 
CAACCGGAAC TGTTGCAGAG CACGAAGAAG ACGGCTTTAT CGAAATTTCT GCAGCTGCAG 
CTGAACGCTT CCCTCTCGCC AGGATCACCC ATTGCCCGGC GAACCAAACA CGAGATTAAG 
CTGTCCCAGA AGATGGCCAG CCGTTGTCAG CAGCATCCGG AGGCGTGGTC CAAATACTTG 
TTGGCCACCT GTTACTCGCT GTACTTCCTC ATCCTGCCAT CCATGGTCTT GGATCCGCGG 
CACGCCGGCA AGGAGCCAGA GATACTGCGC GCCGCCTACG ATGTCCTCGT GCGAGCCAGT 
CGTCTAAAGA TCACCTGTGA CGAGTTCTGC TACAGGATCA TGATGCAGCT ATGCGGTATT 
CACAATCTGC CTGTGCTGGC CGTCCGTCTG CACTACCTGA TGAAACGCTC TGGCGTGCAG 
GCCAATGCGC TCACCTATGG CTTCTACAAC CGCTGCGTCC TTGAGTCGCA GTGGCCCCAG 
GACAGTACCA CGATCAGCCA GATCCGCTGG AATCGCATTA AGAACGTGGT GTTGGGAGCG 
GCGCAGTTCC GCAAGGCTGG CAAACAAAGG GCCGCCTCCA AGGTGAACAA GTCGCTCAGT 
GCGTCCCAGG ACCAGAACCT CAGCACCCTG GAAACGGTGG ACGGACAGTC GCGCACCAGT 
TTGGCTTCCT CCTCCGGCGA TGGCGGTGGC CATGGACTGC TCGATTTTGC CGCCTTCGAT 
CGGCTCAGGA ACAAGTTGGG CAGCATTGTG CGGCAGACGG TGACGGGCGG CAATAACGAG 
ACAATGGGCG ATGCGGTCAA CAACACTGGG TTGCTCATAC CCGGCGAACT CAGCGCCCCG 
AATACGCCGA CCTATGGCGG TGACACCGAA ATCCTGGCCA AGGCACTTCA GCAACAGCAG 
CCACGCAAAC AGATCATGAG CATTGGCGGC GGCGATGACG ATGATGAGGA TGAGGACGAA 
GATGAATACA CGGCCGGATC GCCGAGCACG CCGCAAAAGC AACTGGAAGC GGGCGCCGAT 
GACCTGGAAT ACGCCGGTGG AGGAGACTAC GAGGCGGACG AGGAGGACGA AGATGAAGTC 
GACGAGCATG TGGCCGCACA GCGGGCACGT CAGCGAGTGC AGAGTCCAAC CAAAATCTCG 
CCACGCACGC CCGTGACCCA AAACGATCCA CTGGGTGCTC TCAACGAGGA GGAGTCCGCC 
GCCAGTGCCA CGCCCACGCA GGAGACGCAG CAGGAGCAGC AGCACTCACA GTCGCAGATC 
GACAGCAGCA TATACAGCGA CAAGCCGATC CTGTTCCGGG GCCAGAGGAG CGCCACTTTC 
GACGAGTCGA CGCAGATCGG GAAGAGTATG CACCGTTCGG AAACGATGCC GGTGGCCAGC 
AGCGGTGTGA CCAACAGCCT GGCCAACATC GGATCCTCGC TGAAGTTCAC GTTCGGTCCC 
AGCTTGACGG GCAAGAAGTC GAACGAGTTG ATCCAGGGCA GTCTGAGCAG CATCAAATCG 
GCGGCCAATT CGCTGACCAA AAAGTTCGAC GAGATCAAAG GCGTGATATC GGCCAATTCA 
ACGCCGACGA AAACGAACAA TGGACACCAT CCGCATGGCC TGCATCATGG TCACCATCAT 
CCGCACCACC ACCATCATCA TCATTCGCAG CACGGCAATG CGGAGCAGGA GGAGCACGAT 
GCCGCCGTCC ACGAGGAGGG CAAGCTGCGT CGCGTCTCAA GCGACCTGGA TCCATGGGGC 
AGGCTCAGCG AGTCGCGTAA ATCCAGCTAC AATAACCTCG TGCCGCTCGG CGAGAACAGC 
TCCACCGGCG CCCTTCACAT GCACGCCTTT CCCGCTGTGC CCGACAACCT CTATAGTCTA 
ACAAGCGAGA ACGCCGCGGA TCGTGACTGC GATGTGCTGA TCCAGCTGAC GACCTGCTCG 
CAGTGCCACA ATTGCTCGGT GCTCGTCTAC GACGAGGAGA TCATGAGCGG CTGGACGGCG 
GAGGACTCTA ACCTGAACAC CACCTGCCAT GCCTGCAACA AGCTCACGGT GCCTTTCCTC 
AGCGTACAGA TCGAACGGCA GGTGGAGGAG TCGGAACAGT CGGATCCGCT GCAGGACGGC 
AAGGAGCAAA TAGCCAACGG CAATAGCCAC AAGACCAGCC TGACCGTGCC GTATCTAAAT 
CCGCTGGTGC TGCGCAAGGA GCTGGAGAAC ATACTCACGC AGGAGGGCGA CATTGCGCTG 
ATAAAACCGG AATTTGTCGA TGAGCACCCG ATCATCTATT GGAACTTGCT CTGGCTGATG 
GAGCGCATCG AGAGCAAGAC GCACCTGCCC GAGCTCTGTC TGCCCGTGCC GAGCGACAAG 
GAGCACATCG ATCCGCTGAG CAAGGTGAAG ACGGTGCACA TCCAGTGCCT GTGGGACAAT 
CTGAGCCTGC ATACGGAGGC CAGTGGTCCA CCCATGTACT TGCTGTACAG GGAGACCCAG 
CCGACGAGTC CGCTCATCAA GGCGCTGCTC ACCGATCAGG CGCAGCTCAA CAAGAATGTC 
ATTCAGCAAA TCATTTCGGC CATCCGGTGC AATGACTTCG CCACTCCTCT CAAGCGCTTG 
GCCAATGAGC GACACAAGCT GAAGAGCAAC GGCGTGGAGC GATCGCACTC GTTCTATCGC 
GACATACTCT TCCTGGCCCT GACCGCCATC GGTAGGTCCA ACGTGGACCT GGCCACATTC 
CATCGCGAGT ATGCAGCCGT GTTCGACAAG CTGACGGAGC GGGAGTGCAA CATGTACTAT 
CGCAATCAGG ATCTGCCGCC CTCGGCATCG ACGATCTTCT GTCGTGCTTA CTTCCGCCCG 
CTTCTGCTGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Crag cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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