DENND4A gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005254120.4	XP_005254177.1	C-myc promoter-binding protein isoform X1
XM_017021862.1	XP_016877351.1	C-myc promoter-binding protein isoform X1
XM_005254121.4	XP_005254178.1	C-myc promoter-binding protein isoform X2
XM_005254120.5	XP_005254177.1	C-myc promoter-binding protein isoform X1
XM_024449819.1	XP_024305587.1	C-myc promoter-binding protein isoform X7
NM_001320835.1	NP_001307764.1	C-myc promoter-binding protein isoform 3
NM_001376920.1	NP_001363849.1	C-myc promoter-binding protein isoform 2
NM_005848.4	NP_005839.3	C-myc promoter-binding protein isoform 2
XM_005254121.3	XP_005254178.1	C-myc promoter-binding protein isoform X2
XM_017021865.1	XP_016877354.1	C-myc promoter-binding protein isoform X7
XM_006720371.4	XP_006720434.1	C-myc promoter-binding protein isoform X1
XM_017021865.2	XP_016877354.1	C-myc promoter-binding protein isoform X8
NM_001376919.1	NP_001363848.1	C-myc promoter-binding protein isoform 2
XM_017021862.2	XP_016877351.1	C-myc promoter-binding protein isoform X1
XM_017021864.2	XP_016877353.1	C-myc promoter-binding protein isoform X3
XM_017021863.1	XP_016877352.1	C-myc promoter-binding protein isoform X2
XM_017021864.1	XP_016877353.1	C-myc promoter-binding protein isoform X3
XM_005254122.4	XP_005254179.1	C-myc promoter-binding protein isoform X6
XM_011521157.2	XP_011519459.1	C-myc promoter-binding protein isoform X5
XM_017021863.2	XP_016877352.1	C-myc promoter-binding protein isoform X2
XM_011521156.3	XP_011519458.1	C-myc promoter-binding protein isoform X4
XM_011521157.3	XP_011519459.1	C-myc promoter-binding protein isoform X5
XM_006720371.3	XP_006720434.1	C-myc promoter-binding protein isoform X1
XM_011521156.2	XP_011519458.1	C-myc promoter-binding protein isoform X4
NM_001144823.2	NP_001138295.1	C-myc promoter-binding protein isoform 1
XM_005254122.5	XP_005254179.1	C-myc promoter-binding protein isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu103982	XM_024449819.1 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu85228	XM_017021865.2 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu48055	XM_005254122.5 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu22718	NM_001376919.1 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu22718	NM_001376920.1 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85227	XM_017021864.2 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu22448	NM_001144823.2 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu22718	NM_005848.4 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu48054	NM_001320835.1 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu22718	XM_017021863.2 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu22718	XM_005254121.4 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu48054	XM_005254120.5 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu48054	XM_006720371.4 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu48054	XM_017021862.2 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu63822	XM_011521156.3 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu63823	XM_011521157.3 Latest version!	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu48054	XM_005254120.4	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu22718	XM_005254121.3	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu85228	XM_017021865.1	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu48054	XM_017021862.1	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu85227	XM_017021864.1	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu22718	XM_017021863.1	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu63823	XM_011521157.2	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu48054	XM_006720371.3	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu63822	XM_011521156.2	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu48055	XM_005254122.4	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

DENND4A ( XM_024449819.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305587.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu103982
Clone ID Related Accession (Same CDS sequence)	XM_024449819.1
Accession Version	XM_024449819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGA TGGCAGTGAC CTGGATGCTG TTAGTCATGG CAGCATGGAT 
AGTGGTCATG GGACACACAC TGTGGAGCAG GCACCTTTTA ATACGGGTTT AATCAAAGTA 
TATGCTACTG ATGATAGATC TAGTACAGGT GGCCAGTCTG ATCTTGGATA TAATTCATTA 
TCTAAAGATG AAGTTAGAAG AGGGGATACA TCTACTGAAG ACATTCAAGA AGAAAAAGAT 
AAAAAAGGGA GTGATTGTAG TTCCTTGTCA GAGAGTGAGA GTACAAAAGG AAGTGCTGAT 
TGCCTTCCTA AGCTCAGTTA TCAAAATTCT TCCAGTATCG TTCGCCTCAC TGGTACAAGT 
AACAACAGTG CTGGTAAAAT ATCAGGAGAA AGCATGGAAA GCACACCTGA GCTGCTCTTA 
ATATCATCTC TTGAAGATAC AAATGAAACA CGAAACATTC AAAGTAGATG CTTCAGGAAA 
AGACATAAAA GTGACAATGA AACTAATTTG CAGCAGCAAG TGGTCTGGGG AAATAGAAAC 
CGTAATCTTA GTGGAGGGGT ACTGATGGGA TTTATGCTCA ATAGAATTAA CCAGGAAGCA 
ACCCCTGGAG ATATAGTTGA AAAATTGGGA GCTGATGCAA AAATTCTTTC AAATGTTATC 
TCAAAAAGCA CGAGACCAAA TACTCTTGAT ATTGGGAAGC CACCTTTAAG ATCAAAAAGA 
GACAGTCTAG AAAAGGAATC TAGTGATGAT GATACACCTT TTGATGGTTC TAACTATTTG 
GCAGATAAAG TGGATTCTCC TGTTATTTTT GACTTAGAAG ACTTAGACAG TGAAACAGAT 
GTATCAAAAG CAGGATGTGT TGCTACACAA AATCCCAAGA GGATTCAACG TATGAACAGC 
AGCTTTTCAG TAAAACCTTT TGAAAAAACT GATGTCGCAA CAGGATTTGA TCCCCTCTCT 
CTTTTGGTTG CTGAGACTGA ACAGCAGCAA AAAGAAGAAG AGGAGGAGGA TGAAGATGAT 
AGCAAAAGCA TTTCAACACC ATCTGCTAGG CGTGATTTAG CTGAAGAAAT TGTGATGTAT 
ATGAATAACA TGAGCAGTCC TTTGACAAGT CGTACACCAA GCATTGATTT ACAACGGGCA 
TGTGATGATA AATTAAATAA GAAAAGTCCA CCATTGGTCA AGGCATGCAG AAGATCTAGT 
CTGCCTCCTA ATTCTCCTAA GCCAGTGAGA CTTACCAAAT CTAAAAGTTA CACAAAAAGT 
GAGGAGAAAC CAAGGGATAG ACTTTGGTCT TCTCCAGCCT TCTCACCTAC TTGCCCATTT 
AGGGAAGAAT CTCAGGATAC ATTAACCCAC TCATCACCTT CATTTAACTT AGATACACTA 
CTAGTACCTA AACTAGATGT TCTAAGAAAC AGTATGTTCA CTGCTGGAAA AGGAGTTGCA 
GAGAAAGCAA GCAAATGGTA TTCAAGATTC ACCATGTACA CCACATCATC TAAGGATCAA 
AGTTCTGATC GTACCAGTCT TTCTTCAGTG GGAGCCCAGG ATTCTGAATC TACCTCTTTA 
ACAGATGAAG ATGTCTGCCA TGAGTTGGAA GGACCTATCT CCTCTCAAGA GACCAGTGCT 
ACTTCAGGGA CTAAGAGAAT TGATCTCAGC CGAATAAGCC TGGAAAGTTC TGCATCCTTG 
GAAGGATCTC TGTCGAAGTT TGCCTTACCT GGGAAATCAG AAGTGACATC TTCCTTCAAC 
GCGAGTAATA CAAATATCTT CCAGAACTAT GCAATGGAGG TTCTCATCTC AAGTTGCTCT 
CGGTGTAGAA CTTGTGATTG TCTTGTCCAT GATGAGGAAA TCATGGCTGG CTGGACAGCA 
GATGATTCAA ATCTCAATAC TACATGCCCA TTCTGTGGCA ATATCTTCTT ACCCTTTCTG 
AATATAGAAA TAAGAGATTT AAGACGACCT GGAAGATACT TTCTAAAGTC AAGCCCATCA 
ACAGAAAATA TGCACTTTCC ATCCTCCATT TCAAGTCAGA CGAGGCAGTC TTGCATTTCA 
ACATCAGCCT CTGGTCTTGA CACATCTGCT CTCTCTGTTC AAGGGAATTT TGATCTAAAT 
AGCAAATCTA AACTGCAGGA AAATTTTTGC ACCCGAAGTA TTCAGATCCC TGCTAATAGA 
TCAAAAACAG CTATGTCTAA ATGTCCAATA TTTCCAATGG CCAGGAGTAT TAGTACTTCT 
GGCCCCTTGG ATAAGGAAGA TACTGGAAGA CAGAAGCTCA TTTCTACAGG CAGCCTGCCA 
GCTACTTTAC AAGGAGCTAC AGATTCTTTA GGTTTAGAAT GGCACCTTCC AAGTCCCGAT 
CCTGTCACTG TTCCGTATCT TAGTCCTTTA GTGGTATGGA AAGAACTTGA AAGCTTATTG 
GAAAATGAAG GTGATCATGC AATAACAGTA GCAGACTTTG TGGACCATCA TCCAATTGTT 
TTTTGGAACC TGGTATGGTA TTTCAGACGT CTTGACTTGC CCAGTAACTT ACCTGGATTG 
ATTCTTTCTT CTGAGCACTG TAACAAGTAT TCAAAGATTC CCCGCCACTG TATGTCTGAA 
GATAGCAAAT ATGTTTTAAT ACAAATGTTA TGGGACAATA TGAAGTTACA TCAGGATCCG 
GGACAGCCCT TGTACATTCT CTGGAATGCA CACACCCAAA AATATCCAAT GGTCCATTTA 
TTGCAAAAAA GTGATAACTC ATTTAACCAG GAACTGTTGA AAAGTATGGT AAAAAGCATT 
AAAATGAATG ATGTCTATGG ACCAATGAGT CAGATTTTAG AGACACTGAA TAAGTGTCCA 
CATTTTAAAA GACAGAGGAG TTTATACAGA GAAATACTAT TTCTCTCACT TGTGGCACTG 
GGAAGAGAAA ACATTGATAT AGATGCTTTT GATAAAGAGT ACAAGATGGC ATACGATCGT 
CTCACACCTA GTCAAGTCAA GAGTACACAC AACTGTGATA GACCACCAAG TACAGGGGTG 
ATGGAATGTC GAAAAACCTT TGGAGAACCT TATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305587.1
CDS	2519..6037
Translation	MFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_024449819.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024449819.1

ORF Insert Sequence:

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGA TGGCAGTGAC CTGGATGCTG TTAGTCATGG CAGCATGGAT 
AGTGGTCATG GGACACACAC TGTGGAGCAG GCACCTTTTA ATACGGGTTT AATCAAAGTA 
TATGCTACTG ATGATAGATC TAGTACAGGT GGCCAGTCTG ATCTTGGATA TAATTCATTA 
TCTAAAGATG AAGTTAGAAG AGGGGATACA TCTACTGAAG ACATTCAAGA AGAAAAAGAT 
AAAAAAGGGA GTGATTGTAG TTCCTTGTCA GAGAGTGAGA GTACAAAAGG AAGTGCTGAT 
TGCCTTCCTA AGCTCAGTTA TCAAAATTCT TCCAGTATCG TTCGCCTCAC TGGTACAAGT 
AACAACAGTG CTGGTAAAAT ATCAGGAGAA AGCATGGAAA GCACACCTGA GCTGCTCTTA 
ATATCATCTC TTGAAGATAC AAATGAAACA CGAAACATTC AAAGTAGATG CTTCAGGAAA 
AGACATAAAA GTGACAATGA AACTAATTTG CAGCAGCAAG TGGTCTGGGG AAATAGAAAC 
CGTAATCTTA GTGGAGGGGT ACTGATGGGA TTTATGCTCA ATAGAATTAA CCAGGAAGCA 
ACCCCTGGAG ATATAGTTGA AAAATTGGGA GCTGATGCAA AAATTCTTTC AAATGTTATC 
TCAAAAAGCA CGAGACCAAA TACTCTTGAT ATTGGGAAGC CACCTTTAAG ATCAAAAAGA 
GACAGTCTAG AAAAGGAATC TAGTGATGAT GATACACCTT TTGATGGTTC TAACTATTTG 
GCAGATAAAG TGGATTCTCC TGTTATTTTT GACTTAGAAG ACTTAGACAG TGAAACAGAT 
GTATCAAAAG CAGGATGTGT TGCTACACAA AATCCCAAGA GGATTCAACG TATGAACAGC 
AGCTTTTCAG TAAAACCTTT TGAAAAAACT GATGTCGCAA CAGGATTTGA TCCCCTCTCT 
CTTTTGGTTG CTGAGACTGA ACAGCAGCAA AAAGAAGAAG AGGAGGAGGA TGAAGATGAT 
AGCAAAAGCA TTTCAACACC ATCTGCTAGG CGTGATTTAG CTGAAGAAAT TGTGATGTAT 
ATGAATAACA TGAGCAGTCC TTTGACAAGT CGTACACCAA GCATTGATTT ACAACGGGCA 
TGTGATGATA AATTAAATAA GAAAAGTCCA CCATTGGTCA AGGCATGCAG AAGATCTAGT 
CTGCCTCCTA ATTCTCCTAA GCCAGTGAGA CTTACCAAAT CTAAAAGTTA CACAAAAAGT 
GAGGAGAAAC CAAGGGATAG ACTTTGGTCT TCTCCAGCCT TCTCACCTAC TTGCCCATTT 
AGGGAAGAAT CTCAGGATAC ATTAACCCAC TCATCACCTT CATTTAACTT AGATACACTA 
CTAGTACCTA AACTAGATGT TCTAAGAAAC AGTATGTTCA CTGCTGGAAA AGGAGTTGCA 
GAGAAAGCAA GCAAATGGTA TTCAAGATTC ACCATGTACA CCACATCATC TAAGGATCAA 
AGTTCTGATC GTACCAGTCT TTCTTCAGTG GGAGCCCAGG ATTCTGAATC TACCTCTTTA 
ACAGATGAAG ATGTCTGCCA TGAGTTGGAA GGACCTATCT CCTCTCAAGA GACCAGTGCT 
ACTTCAGGGA CTAAGAGAAT TGATCTCAGC CGAATAAGCC TGGAAAGTTC TGCATCCTTG 
GAAGGATCTC TGTCGAAGTT TGCCTTACCT GGGAAATCAG AAGTGACATC TTCCTTCAAC 
GCGAGTAATA CAAATATCTT CCAGAACTAT GCAATGGAGG TTCTCATCTC AAGTTGCTCT 
CGGTGTAGAA CTTGTGATTG TCTTGTCCAT GATGAGGAAA TCATGGCTGG CTGGACAGCA 
GATGATTCAA ATCTCAATAC TACATGCCCA TTCTGTGGCA ATATCTTCTT ACCCTTTCTG 
AATATAGAAA TAAGAGATTT AAGACGACCT GGAAGATACT TTCTAAAGTC AAGCCCATCA 
ACAGAAAATA TGCACTTTCC ATCCTCCATT TCAAGTCAGA CGAGGCAGTC TTGCATTTCA 
ACATCAGCCT CTGGTCTTGA CACATCTGCT CTCTCTGTTC AAGGGAATTT TGATCTAAAT 
AGCAAATCTA AACTGCAGGA AAATTTTTGC ACCCGAAGTA TTCAGATCCC TGCTAATAGA 
TCAAAAACAG CTATGTCTAA ATGTCCAATA TTTCCAATGG CCAGGAGTAT TAGTACTTCT 
GGCCCCTTGG ATAAGGAAGA TACTGGAAGA CAGAAGCTCA TTTCTACAGG CAGCCTGCCA 
GCTACTTTAC AAGGAGCTAC AGATTCTTTA GGTTTAGAAT GGCACCTTCC AAGTCCCGAT 
CCTGTCACTG TTCCGTATCT TAGTCCTTTA GTGGTATGGA AAGAACTTGA AAGCTTATTG 
GAAAATGAAG GTGATCATGC AATAACAGTA GCAGACTTTG TGGACCATCA TCCAATTGTT 
TTTTGGAACC TGGTATGGTA TTTCAGACGT CTTGACTTGC CCAGTAACTT ACCTGGATTG 
ATTCTTTCTT CTGAGCACTG TAACAAGTAT TCAAAGATTC CCCGCCACTG TATGTCTGAA 
GATAGCAAAT ATGTTTTAAT ACAAATGTTA TGGGACAATA TGAAGTTACA TCAGGATCCG 
GGACAGCCCT TGTACATTCT CTGGAATGCA CACACCCAAA AATATCCAAT GGTCCATTTA 
TTGCAAAAAA GTGATAACTC ATTTAACCAG GAACTGTTGA AAAGTATGGT AAAAAGCATT 
AAAATGAATG ATGTCTATGG ACCAATGAGT CAGATTTTAG AGACACTGAA TAAGTGTCCA 
CATTTTAAAA GACAGAGGAG TTTATACAGA GAAATACTAT TTCTCTCACT TGTGGCACTG 
GGAAGAGAAA ACATTGATAT AGATGCTTTT GATAAAGAGT ACAAGATGGC ATACGATCGT 
CTCACACCTA GTCAAGTCAA GAGTACACAC AACTGTGATA GACCACCAAG TACAGGGGTG 
ATGGAATGTC GAAAAACCTT TGGAGAACCT TATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_017021865.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877354.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu85228
Clone ID Related Accession (Same CDS sequence)	XM_017021865.1 , XM_017021865.2
Accession Version	XM_017021865.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGG TGGCCAGTCT GATCTTGGAT ATAATTCATT ATCTAAAGAT 
GAAGTTAGAA GAGGGGATAC ATCTACTGAA GACATTCAAG AAGAAAAAGA TAAAAAAGGG 
AGTGATTGTA GTTCCTTGTC AGAGAGTGAG AGTACAAAAG GAAGTGCTGA TTGCCTTCCT 
AAGCTCAGTT ATCAAAATTC TTCCAGTATC GTTCGCCTCA CTGGTACAAG TAACAACAGT 
GCTGGTAAAA TATCAGGAGA AAGCATGGAA AGCACACCTG AGCTGCTCTT AATATCATCT 
CTTGAAGATA CAAATGAAAC ACGAAACATT CAAAGTAGAT GCTTCAGGAA AAGACATAAA 
AGTGACAATG AAACTAATTT GCAGCAGCAA GTGGTCTGGG GAAATAGAAA CCGTAATCTT 
AGTGGAGGGG TACTGATGGG ATTTATGCTC AATAGAATTA ACCAGGAAGC AACCCCTGGA 
GATATAGTTG AAAAATTGGG AGCTGATGCA AAAATTCTTT CAAATGTTAT CTCAAAAAGC 
ACGAGACCAA ATACTCTTGA TATTGGGAAG CCACCTTTAA GATCAAAAAG AGACAGTCTA 
GAAAAGGAAT CTAGTGATGA TGATACACCT TTTGATGGTT CTAACTATTT GGCAGATAAA 
GTGGATTCTC CTGTTATTTT TGACTTAGAA GACTTAGACA GTGAAACAGA TGTATCAAAA 
GCAGGATGTG TTGCTACACA AAATCCCAAG AGGATTCAAC GTATGAACAG CAGCTTTTCA 
GTAAAACCTT TTGAAAAAAC TGATGTCGCA ACAGGATTTG ATCCCCTCTC TCTTTTGGTT 
GCTGAGACTG AACAGCAGCA AAAAGAAGAA GAGGAGGAGG ATGAAGATGA TAGCAAAAGC 
ATTTCAACAC CATCTGCTAG GCGTGATTTA GCTGAAGAAA TTGTGATGTA TATGAATAAC 
ATGAGCAGTC CTTTGACAAG TCGTACACCA AGCATTGATT TACAACGGGC ATGTGATGAT 
AAATTAAATA AGAAAAGTCC ACCATTGGTC AAGGCATGCA GAAGATCTAG TCTGCCTCCT 
AATTCTCCTA AGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGAGAAA 
CCAAGGGATA GACTTTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAGGATA CATTAACCCA CTCATCACCT TCATTTAACT TAGATACACT ACTAGTACCT 
AAACTAGATG TTCTAAGAAA CAGTATGTTC ACTGCTGGAA AAGGAGTTGC AGAGAAAGCA 
AGCAAATGGT ATTCAAGATT CACCATGTAC ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTCTGCC ATGAGTTGGA AGGACCTATC TCCTCTCAAG AGACCAGTGC TACTTCAGGG 
ACTAAGAGAA TTGATCTCAG CCGAATAAGC CTGGAAAGTT CTGCATCCTT GGAAGGATCT 
CTGTCGAAGT TTGCCTTACC TGGGAAATCA GAAGTGACAT CTTCCTTCAA CGCGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC ATTCTGTGGC AATATCTTCT TACCCTTTCT GAATATAGAA 
ATAAGAGATT TAAGACGACC TGGAAGATAC TTTCTAAAGT CAAGCCCATC AACAGAAAAT 
ATGCACTTTC CATCCTCCAT TTCAAGTCAG ACGAGGCAGT CTTGCATTTC AACATCAGCC 
TCTGGTCTTG ACACATCTGC TCTCTCTGTT CAAGGGAATT TTGATCTAAA TAGCAAATCT 
AAACTGCAGG AAAATTTTTG CACCCGAAGT ATTCAGATCC CTGCTAATAG ATCAAAAACA 
GCTATGTCTA AATGTCCAAT ATTTCCAATG GCCAGGAGTA TTAGTACTTC TGGCCCCTTG 
GATAAGGAAG ATACTGGAAG ACAGAAGCTC ATTTCTACAG GCAGCCTGCC AGCTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGTTTAGAA TGGCACCTTC CAAGTCCCGA TCCTGTCACT 
GTTCCGTATC TTAGTCCTTT AGTGGTATGG AAAGAACTTG AAAGCTTATT GGAAAATGAA 
GGTGATCATG CAATAACAGT AGCAGACTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTGGTATGGT ATTTCAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGCAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAGTTAC ATCAGGATCC GGGACAGCCC 
TTGTACATTC TCTGGAATGC ACACACCCAA AAATATCCAA TGGTCCATTT ATTGCAAAAA 
AGTGATAACT CATTTAACCA GGAACTGTTG AAAAGTATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAGTGTCC ACATTTTAAA 
AGACAGAGGA GTTTATACAG AGAAATACTA TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGATA TAGATGCTTT TGATAAAGAG TACAAGATGG CATACGATCG TCTCACACCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACAGGGGT GATGGAATGT 
CGAAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877354.1
CDS	2569..5958
Translation	MFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_017021865.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021865.1

ORF Insert Sequence:

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGG TGGCCAGTCT GATCTTGGAT ATAATTCATT ATCTAAAGAT 
GAAGTTAGAA GAGGGGATAC ATCTACTGAA GACATTCAAG AAGAAAAAGA TAAAAAAGGG 
AGTGATTGTA GTTCCTTGTC AGAGAGTGAG AGTACAAAAG GAAGTGCTGA TTGCCTTCCT 
AAGCTCAGTT ATCAAAATTC TTCCAGTATC GTTCGCCTCA CTGGTACAAG TAACAACAGT 
GCTGGTAAAA TATCAGGAGA AAGCATGGAA AGCACACCTG AGCTGCTCTT AATATCATCT 
CTTGAAGATA CAAATGAAAC ACGAAACATT CAAAGTAGAT GCTTCAGGAA AAGACATAAA 
AGTGACAATG AAACTAATTT GCAGCAGCAA GTGGTCTGGG GAAATAGAAA CCGTAATCTT 
AGTGGAGGGG TACTGATGGG ATTTATGCTC AATAGAATTA ACCAGGAAGC AACCCCTGGA 
GATATAGTTG AAAAATTGGG AGCTGATGCA AAAATTCTTT CAAATGTTAT CTCAAAAAGC 
ACGAGACCAA ATACTCTTGA TATTGGGAAG CCACCTTTAA GATCAAAAAG AGACAGTCTA 
GAAAAGGAAT CTAGTGATGA TGATACACCT TTTGATGGTT CTAACTATTT GGCAGATAAA 
GTGGATTCTC CTGTTATTTT TGACTTAGAA GACTTAGACA GTGAAACAGA TGTATCAAAA 
GCAGGATGTG TTGCTACACA AAATCCCAAG AGGATTCAAC GTATGAACAG CAGCTTTTCA 
GTAAAACCTT TTGAAAAAAC TGATGTCGCA ACAGGATTTG ATCCCCTCTC TCTTTTGGTT 
GCTGAGACTG AACAGCAGCA AAAAGAAGAA GAGGAGGAGG ATGAAGATGA TAGCAAAAGC 
ATTTCAACAC CATCTGCTAG GCGTGATTTA GCTGAAGAAA TTGTGATGTA TATGAATAAC 
ATGAGCAGTC CTTTGACAAG TCGTACACCA AGCATTGATT TACAACGGGC ATGTGATGAT 
AAATTAAATA AGAAAAGTCC ACCATTGGTC AAGGCATGCA GAAGATCTAG TCTGCCTCCT 
AATTCTCCTA AGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGAGAAA 
CCAAGGGATA GACTTTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAGGATA CATTAACCCA CTCATCACCT TCATTTAACT TAGATACACT ACTAGTACCT 
AAACTAGATG TTCTAAGAAA CAGTATGTTC ACTGCTGGAA AAGGAGTTGC AGAGAAAGCA 
AGCAAATGGT ATTCAAGATT CACCATGTAC ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTCTGCC ATGAGTTGGA AGGACCTATC TCCTCTCAAG AGACCAGTGC TACTTCAGGG 
ACTAAGAGAA TTGATCTCAG CCGAATAAGC CTGGAAAGTT CTGCATCCTT GGAAGGATCT 
CTGTCGAAGT TTGCCTTACC TGGGAAATCA GAAGTGACAT CTTCCTTCAA CGCGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC ATTCTGTGGC AATATCTTCT TACCCTTTCT GAATATAGAA 
ATAAGAGATT TAAGACGACC TGGAAGATAC TTTCTAAAGT CAAGCCCATC AACAGAAAAT 
ATGCACTTTC CATCCTCCAT TTCAAGTCAG ACGAGGCAGT CTTGCATTTC AACATCAGCC 
TCTGGTCTTG ACACATCTGC TCTCTCTGTT CAAGGGAATT TTGATCTAAA TAGCAAATCT 
AAACTGCAGG AAAATTTTTG CACCCGAAGT ATTCAGATCC CTGCTAATAG ATCAAAAACA 
GCTATGTCTA AATGTCCAAT ATTTCCAATG GCCAGGAGTA TTAGTACTTC TGGCCCCTTG 
GATAAGGAAG ATACTGGAAG ACAGAAGCTC ATTTCTACAG GCAGCCTGCC AGCTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGTTTAGAA TGGCACCTTC CAAGTCCCGA TCCTGTCACT 
GTTCCGTATC TTAGTCCTTT AGTGGTATGG AAAGAACTTG AAAGCTTATT GGAAAATGAA 
GGTGATCATG CAATAACAGT AGCAGACTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTGGTATGGT ATTTCAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGCAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAGTTAC ATCAGGATCC GGGACAGCCC 
TTGTACATTC TCTGGAATGC ACACACCCAA AAATATCCAA TGGTCCATTT ATTGCAAAAA 
AGTGATAACT CATTTAACCA GGAACTGTTG AAAAGTATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAGTGTCC ACATTTTAAA 
AGACAGAGGA GTTTATACAG AGAAATACTA TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGATA TAGATGCTTT TGATAAAGAG TACAAGATGG CATACGATCG TCTCACACCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACAGGGGT GATGGAATGT 
CGAAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_017021865.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877354.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X13, mRNA.

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CloneID	OHu85228
Clone ID Related Accession (Same CDS sequence)	XM_017021865.1 , XM_017021865.2
Accession Version	XM_017021865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGG TGGCCAGTCT GATCTTGGAT ATAATTCATT ATCTAAAGAT 
GAAGTTAGAA GAGGGGATAC ATCTACTGAA GACATTCAAG AAGAAAAAGA TAAAAAAGGG 
AGTGATTGTA GTTCCTTGTC AGAGAGTGAG AGTACAAAAG GAAGTGCTGA TTGCCTTCCT 
AAGCTCAGTT ATCAAAATTC TTCCAGTATC GTTCGCCTCA CTGGTACAAG TAACAACAGT 
GCTGGTAAAA TATCAGGAGA AAGCATGGAA AGCACACCTG AGCTGCTCTT AATATCATCT 
CTTGAAGATA CAAATGAAAC ACGAAACATT CAAAGTAGAT GCTTCAGGAA AAGACATAAA 
AGTGACAATG AAACTAATTT GCAGCAGCAA GTGGTCTGGG GAAATAGAAA CCGTAATCTT 
AGTGGAGGGG TACTGATGGG ATTTATGCTC AATAGAATTA ACCAGGAAGC AACCCCTGGA 
GATATAGTTG AAAAATTGGG AGCTGATGCA AAAATTCTTT CAAATGTTAT CTCAAAAAGC 
ACGAGACCAA ATACTCTTGA TATTGGGAAG CCACCTTTAA GATCAAAAAG AGACAGTCTA 
GAAAAGGAAT CTAGTGATGA TGATACACCT TTTGATGGTT CTAACTATTT GGCAGATAAA 
GTGGATTCTC CTGTTATTTT TGACTTAGAA GACTTAGACA GTGAAACAGA TGTATCAAAA 
GCAGGATGTG TTGCTACACA AAATCCCAAG AGGATTCAAC GTATGAACAG CAGCTTTTCA 
GTAAAACCTT TTGAAAAAAC TGATGTCGCA ACAGGATTTG ATCCCCTCTC TCTTTTGGTT 
GCTGAGACTG AACAGCAGCA AAAAGAAGAA GAGGAGGAGG ATGAAGATGA TAGCAAAAGC 
ATTTCAACAC CATCTGCTAG GCGTGATTTA GCTGAAGAAA TTGTGATGTA TATGAATAAC 
ATGAGCAGTC CTTTGACAAG TCGTACACCA AGCATTGATT TACAACGGGC ATGTGATGAT 
AAATTAAATA AGAAAAGTCC ACCATTGGTC AAGGCATGCA GAAGATCTAG TCTGCCTCCT 
AATTCTCCTA AGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGAGAAA 
CCAAGGGATA GACTTTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAGGATA CATTAACCCA CTCATCACCT TCATTTAACT TAGATACACT ACTAGTACCT 
AAACTAGATG TTCTAAGAAA CAGTATGTTC ACTGCTGGAA AAGGAGTTGC AGAGAAAGCA 
AGCAAATGGT ATTCAAGATT CACCATGTAC ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTCTGCC ATGAGTTGGA AGGACCTATC TCCTCTCAAG AGACCAGTGC TACTTCAGGG 
ACTAAGAGAA TTGATCTCAG CCGAATAAGC CTGGAAAGTT CTGCATCCTT GGAAGGATCT 
CTGTCGAAGT TTGCCTTACC TGGGAAATCA GAAGTGACAT CTTCCTTCAA CGCGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC ATTCTGTGGC AATATCTTCT TACCCTTTCT GAATATAGAA 
ATAAGAGATT TAAGACGACC TGGAAGATAC TTTCTAAAGT CAAGCCCATC AACAGAAAAT 
ATGCACTTTC CATCCTCCAT TTCAAGTCAG ACGAGGCAGT CTTGCATTTC AACATCAGCC 
TCTGGTCTTG ACACATCTGC TCTCTCTGTT CAAGGGAATT TTGATCTAAA TAGCAAATCT 
AAACTGCAGG AAAATTTTTG CACCCGAAGT ATTCAGATCC CTGCTAATAG ATCAAAAACA 
GCTATGTCTA AATGTCCAAT ATTTCCAATG GCCAGGAGTA TTAGTACTTC TGGCCCCTTG 
GATAAGGAAG ATACTGGAAG ACAGAAGCTC ATTTCTACAG GCAGCCTGCC AGCTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGTTTAGAA TGGCACCTTC CAAGTCCCGA TCCTGTCACT 
GTTCCGTATC TTAGTCCTTT AGTGGTATGG AAAGAACTTG AAAGCTTATT GGAAAATGAA 
GGTGATCATG CAATAACAGT AGCAGACTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTGGTATGGT ATTTCAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGCAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAGTTAC ATCAGGATCC GGGACAGCCC 
TTGTACATTC TCTGGAATGC ACACACCCAA AAATATCCAA TGGTCCATTT ATTGCAAAAA 
AGTGATAACT CATTTAACCA GGAACTGTTG AAAAGTATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAGTGTCC ACATTTTAAA 
AGACAGAGGA GTTTATACAG AGAAATACTA TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGATA TAGATGCTTT TGATAAAGAG TACAAGATGG CATACGATCG TCTCACACCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACAGGGGT GATGGAATGT 
CGAAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877354.1
CDS	2519..5908
Translation	MFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_017021865.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021865.2

ORF Insert Sequence:

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGG TGGCCAGTCT GATCTTGGAT ATAATTCATT ATCTAAAGAT 
GAAGTTAGAA GAGGGGATAC ATCTACTGAA GACATTCAAG AAGAAAAAGA TAAAAAAGGG 
AGTGATTGTA GTTCCTTGTC AGAGAGTGAG AGTACAAAAG GAAGTGCTGA TTGCCTTCCT 
AAGCTCAGTT ATCAAAATTC TTCCAGTATC GTTCGCCTCA CTGGTACAAG TAACAACAGT 
GCTGGTAAAA TATCAGGAGA AAGCATGGAA AGCACACCTG AGCTGCTCTT AATATCATCT 
CTTGAAGATA CAAATGAAAC ACGAAACATT CAAAGTAGAT GCTTCAGGAA AAGACATAAA 
AGTGACAATG AAACTAATTT GCAGCAGCAA GTGGTCTGGG GAAATAGAAA CCGTAATCTT 
AGTGGAGGGG TACTGATGGG ATTTATGCTC AATAGAATTA ACCAGGAAGC AACCCCTGGA 
GATATAGTTG AAAAATTGGG AGCTGATGCA AAAATTCTTT CAAATGTTAT CTCAAAAAGC 
ACGAGACCAA ATACTCTTGA TATTGGGAAG CCACCTTTAA GATCAAAAAG AGACAGTCTA 
GAAAAGGAAT CTAGTGATGA TGATACACCT TTTGATGGTT CTAACTATTT GGCAGATAAA 
GTGGATTCTC CTGTTATTTT TGACTTAGAA GACTTAGACA GTGAAACAGA TGTATCAAAA 
GCAGGATGTG TTGCTACACA AAATCCCAAG AGGATTCAAC GTATGAACAG CAGCTTTTCA 
GTAAAACCTT TTGAAAAAAC TGATGTCGCA ACAGGATTTG ATCCCCTCTC TCTTTTGGTT 
GCTGAGACTG AACAGCAGCA AAAAGAAGAA GAGGAGGAGG ATGAAGATGA TAGCAAAAGC 
ATTTCAACAC CATCTGCTAG GCGTGATTTA GCTGAAGAAA TTGTGATGTA TATGAATAAC 
ATGAGCAGTC CTTTGACAAG TCGTACACCA AGCATTGATT TACAACGGGC ATGTGATGAT 
AAATTAAATA AGAAAAGTCC ACCATTGGTC AAGGCATGCA GAAGATCTAG TCTGCCTCCT 
AATTCTCCTA AGCCAGTGAG ACTTACCAAA TCTAAAAGTT ACACAAAAAG TGAGGAGAAA 
CCAAGGGATA GACTTTGGTC TTCTCCAGCC TTCTCACCTA CTTGCCCATT TAGGGAAGAA 
TCTCAGGATA CATTAACCCA CTCATCACCT TCATTTAACT TAGATACACT ACTAGTACCT 
AAACTAGATG TTCTAAGAAA CAGTATGTTC ACTGCTGGAA AAGGAGTTGC AGAGAAAGCA 
AGCAAATGGT ATTCAAGATT CACCATGTAC ACCACATCAT CTAAGGATCA AAGTTCTGAT 
CGTACCAGTC TTTCTTCAGT GGGAGCCCAG GATTCTGAAT CTACCTCTTT AACAGATGAA 
GATGTCTGCC ATGAGTTGGA AGGACCTATC TCCTCTCAAG AGACCAGTGC TACTTCAGGG 
ACTAAGAGAA TTGATCTCAG CCGAATAAGC CTGGAAAGTT CTGCATCCTT GGAAGGATCT 
CTGTCGAAGT TTGCCTTACC TGGGAAATCA GAAGTGACAT CTTCCTTCAA CGCGAGTAAT 
ACAAATATCT TCCAGAACTA TGCAATGGAG GTTCTCATCT CAAGTTGCTC TCGGTGTAGA 
ACTTGTGATT GTCTTGTCCA TGATGAGGAA ATCATGGCTG GCTGGACAGC AGATGATTCA 
AATCTCAATA CTACATGCCC ATTCTGTGGC AATATCTTCT TACCCTTTCT GAATATAGAA 
ATAAGAGATT TAAGACGACC TGGAAGATAC TTTCTAAAGT CAAGCCCATC AACAGAAAAT 
ATGCACTTTC CATCCTCCAT TTCAAGTCAG ACGAGGCAGT CTTGCATTTC AACATCAGCC 
TCTGGTCTTG ACACATCTGC TCTCTCTGTT CAAGGGAATT TTGATCTAAA TAGCAAATCT 
AAACTGCAGG AAAATTTTTG CACCCGAAGT ATTCAGATCC CTGCTAATAG ATCAAAAACA 
GCTATGTCTA AATGTCCAAT ATTTCCAATG GCCAGGAGTA TTAGTACTTC TGGCCCCTTG 
GATAAGGAAG ATACTGGAAG ACAGAAGCTC ATTTCTACAG GCAGCCTGCC AGCTACTTTA 
CAAGGAGCTA CAGATTCTTT AGGTTTAGAA TGGCACCTTC CAAGTCCCGA TCCTGTCACT 
GTTCCGTATC TTAGTCCTTT AGTGGTATGG AAAGAACTTG AAAGCTTATT GGAAAATGAA 
GGTGATCATG CAATAACAGT AGCAGACTTT GTGGACCATC ATCCAATTGT TTTTTGGAAC 
CTGGTATGGT ATTTCAGACG TCTTGACTTG CCCAGTAACT TACCTGGATT GATTCTTTCT 
TCTGAGCACT GTAACAAGTA TTCAAAGATT CCCCGCCACT GTATGTCTGA AGATAGCAAA 
TATGTTTTAA TACAAATGTT ATGGGACAAT ATGAAGTTAC ATCAGGATCC GGGACAGCCC 
TTGTACATTC TCTGGAATGC ACACACCCAA AAATATCCAA TGGTCCATTT ATTGCAAAAA 
AGTGATAACT CATTTAACCA GGAACTGTTG AAAAGTATGG TAAAAAGCAT TAAAATGAAT 
GATGTCTATG GACCAATGAG TCAGATTTTA GAGACACTGA ATAAGTGTCC ACATTTTAAA 
AGACAGAGGA GTTTATACAG AGAAATACTA TTTCTCTCAC TTGTGGCACT GGGAAGAGAA 
AACATTGATA TAGATGCTTT TGATAAAGAG TACAAGATGG CATACGATCG TCTCACACCT 
AGTCAAGTCA AGAGTACACA CAACTGTGAT AGACCACCAA GTACAGGGGT GATGGAATGT 
CGAAAAACCT TTGGAGAACC TTATCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005254122.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254179.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu48055
Clone ID Related Accession (Same CDS sequence)	XM_005254122.4 , XM_005254122.5
Accession Version	XM_005254122.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254122.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGC AGATGGCAGT GACCTGGATG CTGTTAGTCA TGGCAGCATG 
GATAGTGGTC ATGGGACACA CACTGTGGAG CAGGCACCTT TTAATACGGG TTTAATCAAA 
GTATATGCTA CTGATGATAG ATCTAGTACA GGTGGCCAGT CTGATCTTGG ATATAATTCA 
TTATCTAAAG ATGAAGTTAG AAGAGGGGAT ACATCTACTG AAGACATTCA AGAAGAAAAA 
GATAAAAAAG GGAGTGATTG TAGTTCCTTG TCAGAGAGTG AGAGTACAAA AGGAAGTGCT 
GATTGCCTTC CTAAGCTCAG TTATCAAAAT TCTTCCAGTA TCGTTCGCCT CACTGGTACA 
AGTAACAACA GTGCTGGTAA AATATCAGGA GAAAGCATGG AAAGCACACC TGAGCTGCTC 
TTAATATCAT CTCTTGAAGA TACAAATGAA ACACGAAACA TTCAAAGTAG ATGCTTCAGG 
AAAAGACATA AAAGTGACAA TGAAACTAAT TTGCAGCAGC AAGTGGTCTG GGGAAATAGA 
AACCGTAATC TTAGTGGAGG GGTACTGATG GGATTTATGC TCAATAGAAT TAACCAGGAA 
GCAACCCCTG GAGATATAGT TGAAAAATTG GGAGCTGATG CAAAAATTCT TTCAAATGTT 
ATCTCAAAAA GCACGAGACC AAATACTCTT GATATTGGGA AGCCACCTTT AAGATCAAAA 
AGAGACAGTC TAGAAAAGGA ATCTAGTGAT GATGATACAC CTTTTGATGG TTCTAACTAT 
TTGGCAGATA AAGTGGATTC TCCTGTTATT TTTGACTTAG AAGACTTAGA CAGTGAAACA 
GATGTATCAA AAGCAGGATG TGTTGCTACA CAAAATCCCA AGAGGATTCA ACGTATGAAC 
AGCAGCTTTT CAGTAAAACC TTTTGAAAAA ACTGATGTCG CAACAGGATT TGATCCCCTC 
TCTCTTTTGG TTGCTGAGAC TGAACAGCAG CAAAAAGAAG AAGAGGAGGA GGATGAAGAT 
GATAGCAAAA GCATTTCAAC ACCATCTGCT AGGCGTGATT TAGCTGAAGA AATTGTGATG 
TATATGAATA ACATGAGCAG TCCTTTGACA AGTCGTACAC CAAGCATTGA TTTACAACGG 
GCATGTGATG ATAAATTAAA TAAGAAAAGT CCACCATTGG TCAAGGCATG CAGAAGATCT 
AGTCTGCCTC CTAATTCTCC TAAGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGAGA AACCAAGGGA TAGACTTTGG TCTTCTCCAG CCTTCTCACC TACTTGCCCA 
TTTAGGGAAG AATCTCAGGA TACATTAACC CACTCATCAC CTTCATTTAA CTTAGATACA 
CTACTAGTAC CTAAACTAGA TGTTCTAAGA AACAGTATGT TCACTGCTGG AAAAGGAGTT 
GCAGAGAAAG CAAGCAAATG GTATTCAAGA TTCACCATGT ACACCACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTACCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTACCTCT 
TTAACAGATG AAGATGTCTG CCATGAGTTG GAAGGACCTA TCTCCTCTCA AGAGACCAGT 
GCTACTTCAG GGACTAAGAG AATTGATCTC AGCCGAATAA GCCTGGAAAG TTCTGCATCC 
TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC 
AACGCGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA TTGTCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGATT CAAATCTCAA TACTACATGC CCATTCTGTG GCAATATCTT CTTACCCTTT 
CTGAATATAG AAATAAGAGA TTTAAGACGA CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA 
TCAACAGAAA ATATGCACTT TCCATCCTCC ATTTCAAGTC AGACGAGGCA GTCTTGCATT 
TCAACATCAG CCTCTGGTCT TGACACATCT GCTCTCTCTG TTCAAGGGAA TTTTGATCTA 
AATAGCAAAT CTAAACTGCA GGAAAATTTT TGCACCCGAA GTATTCAGAT CCCTGCTAAT 
AGATCAAAAA CAGCTATGTC TAAATGTCCA ATATTTCCAA TGGCCAGGAG TATTAGTACT 
TCTGGCCCCT TGGATAAGGA AGATACTGGA AGACAGAAGC TCATTTCTAC AGGCAGCCTG 
CCAGCTACTT TACAAGGAGC TACAGATTCT TTAGGTTTAG AATGGCACCT TCCAAGTCCC 
GATCCTGTCA CTGTTCCGTA TCTTAGTCCT TTAGTGGTAT GGAAAGAACT TGAAAGCTTA 
TTGGAAAATG AAGGTGATCA TGCAATAACA GTAGCAGACT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTGGTATG GTATTTCAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA CTGTAACAAG TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGCA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAGTT ACATCAGGAT 
CCGGGACAGC CCTTGTACAT TCTCTGGAAT GCACACACCC AAAAATATCC AATGGTCCAT 
TTATTGCAAA AAAGTGATAA CTCATTTAAC CAGGAACTGT TGAAAAGTAT GGTAAAAAGC 
ATTAAAATGA ATGATGTCTA TGGACCAATG AGTCAGATTT TAGAGACACT GAATAAGTGT 
CCACATTTTA AAAGACAGAG GAGTTTATAC AGAGAAATAC TATTTCTCTC ACTTGTGGCA 
CTGGGAAGAG AAAACATTGA TATAGATGCT TTTGATAAAG AGTACAAGAT GGCATACGAT 
CGTCTCACAC CTAGTCAAGT CAAGAGTACA CACAACTGTG ATAGACCACC AAGTACAGGG 
GTGATGGAAT GTCGAAAAAC CTTTGGAGAA CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254179.1
CDS	2570..6091
Translation	MFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005254122.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254122.4

ORF Insert Sequence:

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGC AGATGGCAGT GACCTGGATG CTGTTAGTCA TGGCAGCATG 
GATAGTGGTC ATGGGACACA CACTGTGGAG CAGGCACCTT TTAATACGGG TTTAATCAAA 
GTATATGCTA CTGATGATAG ATCTAGTACA GGTGGCCAGT CTGATCTTGG ATATAATTCA 
TTATCTAAAG ATGAAGTTAG AAGAGGGGAT ACATCTACTG AAGACATTCA AGAAGAAAAA 
GATAAAAAAG GGAGTGATTG TAGTTCCTTG TCAGAGAGTG AGAGTACAAA AGGAAGTGCT 
GATTGCCTTC CTAAGCTCAG TTATCAAAAT TCTTCCAGTA TCGTTCGCCT CACTGGTACA 
AGTAACAACA GTGCTGGTAA AATATCAGGA GAAAGCATGG AAAGCACACC TGAGCTGCTC 
TTAATATCAT CTCTTGAAGA TACAAATGAA ACACGAAACA TTCAAAGTAG ATGCTTCAGG 
AAAAGACATA AAAGTGACAA TGAAACTAAT TTGCAGCAGC AAGTGGTCTG GGGAAATAGA 
AACCGTAATC TTAGTGGAGG GGTACTGATG GGATTTATGC TCAATAGAAT TAACCAGGAA 
GCAACCCCTG GAGATATAGT TGAAAAATTG GGAGCTGATG CAAAAATTCT TTCAAATGTT 
ATCTCAAAAA GCACGAGACC AAATACTCTT GATATTGGGA AGCCACCTTT AAGATCAAAA 
AGAGACAGTC TAGAAAAGGA ATCTAGTGAT GATGATACAC CTTTTGATGG TTCTAACTAT 
TTGGCAGATA AAGTGGATTC TCCTGTTATT TTTGACTTAG AAGACTTAGA CAGTGAAACA 
GATGTATCAA AAGCAGGATG TGTTGCTACA CAAAATCCCA AGAGGATTCA ACGTATGAAC 
AGCAGCTTTT CAGTAAAACC TTTTGAAAAA ACTGATGTCG CAACAGGATT TGATCCCCTC 
TCTCTTTTGG TTGCTGAGAC TGAACAGCAG CAAAAAGAAG AAGAGGAGGA GGATGAAGAT 
GATAGCAAAA GCATTTCAAC ACCATCTGCT AGGCGTGATT TAGCTGAAGA AATTGTGATG 
TATATGAATA ACATGAGCAG TCCTTTGACA AGTCGTACAC CAAGCATTGA TTTACAACGG 
GCATGTGATG ATAAATTAAA TAAGAAAAGT CCACCATTGG TCAAGGCATG CAGAAGATCT 
AGTCTGCCTC CTAATTCTCC TAAGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGAGA AACCAAGGGA TAGACTTTGG TCTTCTCCAG CCTTCTCACC TACTTGCCCA 
TTTAGGGAAG AATCTCAGGA TACATTAACC CACTCATCAC CTTCATTTAA CTTAGATACA 
CTACTAGTAC CTAAACTAGA TGTTCTAAGA AACAGTATGT TCACTGCTGG AAAAGGAGTT 
GCAGAGAAAG CAAGCAAATG GTATTCAAGA TTCACCATGT ACACCACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTACCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTACCTCT 
TTAACAGATG AAGATGTCTG CCATGAGTTG GAAGGACCTA TCTCCTCTCA AGAGACCAGT 
GCTACTTCAG GGACTAAGAG AATTGATCTC AGCCGAATAA GCCTGGAAAG TTCTGCATCC 
TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC 
AACGCGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA TTGTCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGATT CAAATCTCAA TACTACATGC CCATTCTGTG GCAATATCTT CTTACCCTTT 
CTGAATATAG AAATAAGAGA TTTAAGACGA CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA 
TCAACAGAAA ATATGCACTT TCCATCCTCC ATTTCAAGTC AGACGAGGCA GTCTTGCATT 
TCAACATCAG CCTCTGGTCT TGACACATCT GCTCTCTCTG TTCAAGGGAA TTTTGATCTA 
AATAGCAAAT CTAAACTGCA GGAAAATTTT TGCACCCGAA GTATTCAGAT CCCTGCTAAT 
AGATCAAAAA CAGCTATGTC TAAATGTCCA ATATTTCCAA TGGCCAGGAG TATTAGTACT 
TCTGGCCCCT TGGATAAGGA AGATACTGGA AGACAGAAGC TCATTTCTAC AGGCAGCCTG 
CCAGCTACTT TACAAGGAGC TACAGATTCT TTAGGTTTAG AATGGCACCT TCCAAGTCCC 
GATCCTGTCA CTGTTCCGTA TCTTAGTCCT TTAGTGGTAT GGAAAGAACT TGAAAGCTTA 
TTGGAAAATG AAGGTGATCA TGCAATAACA GTAGCAGACT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTGGTATG GTATTTCAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA CTGTAACAAG TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGCA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAGTT ACATCAGGAT 
CCGGGACAGC CCTTGTACAT TCTCTGGAAT GCACACACCC AAAAATATCC AATGGTCCAT 
TTATTGCAAA AAAGTGATAA CTCATTTAAC CAGGAACTGT TGAAAAGTAT GGTAAAAAGC 
ATTAAAATGA ATGATGTCTA TGGACCAATG AGTCAGATTT TAGAGACACT GAATAAGTGT 
CCACATTTTA AAAGACAGAG GAGTTTATAC AGAGAAATAC TATTTCTCTC ACTTGTGGCA 
CTGGGAAGAG AAAACATTGA TATAGATGCT TTTGATAAAG AGTACAAGAT GGCATACGAT 
CGTCTCACAC CTAGTCAAGT CAAGAGTACA CACAACTGTG ATAGACCACC AAGTACAGGG 
GTGATGGAAT GTCGAAAAAC CTTTGGAGAA CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005254122.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254179.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu48055
Clone ID Related Accession (Same CDS sequence)	XM_005254122.4 , XM_005254122.5
Accession Version	XM_005254122.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005254122.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGC AGATGGCAGT GACCTGGATG CTGTTAGTCA TGGCAGCATG 
GATAGTGGTC ATGGGACACA CACTGTGGAG CAGGCACCTT TTAATACGGG TTTAATCAAA 
GTATATGCTA CTGATGATAG ATCTAGTACA GGTGGCCAGT CTGATCTTGG ATATAATTCA 
TTATCTAAAG ATGAAGTTAG AAGAGGGGAT ACATCTACTG AAGACATTCA AGAAGAAAAA 
GATAAAAAAG GGAGTGATTG TAGTTCCTTG TCAGAGAGTG AGAGTACAAA AGGAAGTGCT 
GATTGCCTTC CTAAGCTCAG TTATCAAAAT TCTTCCAGTA TCGTTCGCCT CACTGGTACA 
AGTAACAACA GTGCTGGTAA AATATCAGGA GAAAGCATGG AAAGCACACC TGAGCTGCTC 
TTAATATCAT CTCTTGAAGA TACAAATGAA ACACGAAACA TTCAAAGTAG ATGCTTCAGG 
AAAAGACATA AAAGTGACAA TGAAACTAAT TTGCAGCAGC AAGTGGTCTG GGGAAATAGA 
AACCGTAATC TTAGTGGAGG GGTACTGATG GGATTTATGC TCAATAGAAT TAACCAGGAA 
GCAACCCCTG GAGATATAGT TGAAAAATTG GGAGCTGATG CAAAAATTCT TTCAAATGTT 
ATCTCAAAAA GCACGAGACC AAATACTCTT GATATTGGGA AGCCACCTTT AAGATCAAAA 
AGAGACAGTC TAGAAAAGGA ATCTAGTGAT GATGATACAC CTTTTGATGG TTCTAACTAT 
TTGGCAGATA AAGTGGATTC TCCTGTTATT TTTGACTTAG AAGACTTAGA CAGTGAAACA 
GATGTATCAA AAGCAGGATG TGTTGCTACA CAAAATCCCA AGAGGATTCA ACGTATGAAC 
AGCAGCTTTT CAGTAAAACC TTTTGAAAAA ACTGATGTCG CAACAGGATT TGATCCCCTC 
TCTCTTTTGG TTGCTGAGAC TGAACAGCAG CAAAAAGAAG AAGAGGAGGA GGATGAAGAT 
GATAGCAAAA GCATTTCAAC ACCATCTGCT AGGCGTGATT TAGCTGAAGA AATTGTGATG 
TATATGAATA ACATGAGCAG TCCTTTGACA AGTCGTACAC CAAGCATTGA TTTACAACGG 
GCATGTGATG ATAAATTAAA TAAGAAAAGT CCACCATTGG TCAAGGCATG CAGAAGATCT 
AGTCTGCCTC CTAATTCTCC TAAGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGAGA AACCAAGGGA TAGACTTTGG TCTTCTCCAG CCTTCTCACC TACTTGCCCA 
TTTAGGGAAG AATCTCAGGA TACATTAACC CACTCATCAC CTTCATTTAA CTTAGATACA 
CTACTAGTAC CTAAACTAGA TGTTCTAAGA AACAGTATGT TCACTGCTGG AAAAGGAGTT 
GCAGAGAAAG CAAGCAAATG GTATTCAAGA TTCACCATGT ACACCACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTACCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTACCTCT 
TTAACAGATG AAGATGTCTG CCATGAGTTG GAAGGACCTA TCTCCTCTCA AGAGACCAGT 
GCTACTTCAG GGACTAAGAG AATTGATCTC AGCCGAATAA GCCTGGAAAG TTCTGCATCC 
TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC 
AACGCGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA TTGTCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGATT CAAATCTCAA TACTACATGC CCATTCTGTG GCAATATCTT CTTACCCTTT 
CTGAATATAG AAATAAGAGA TTTAAGACGA CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA 
TCAACAGAAA ATATGCACTT TCCATCCTCC ATTTCAAGTC AGACGAGGCA GTCTTGCATT 
TCAACATCAG CCTCTGGTCT TGACACATCT GCTCTCTCTG TTCAAGGGAA TTTTGATCTA 
AATAGCAAAT CTAAACTGCA GGAAAATTTT TGCACCCGAA GTATTCAGAT CCCTGCTAAT 
AGATCAAAAA CAGCTATGTC TAAATGTCCA ATATTTCCAA TGGCCAGGAG TATTAGTACT 
TCTGGCCCCT TGGATAAGGA AGATACTGGA AGACAGAAGC TCATTTCTAC AGGCAGCCTG 
CCAGCTACTT TACAAGGAGC TACAGATTCT TTAGGTTTAG AATGGCACCT TCCAAGTCCC 
GATCCTGTCA CTGTTCCGTA TCTTAGTCCT TTAGTGGTAT GGAAAGAACT TGAAAGCTTA 
TTGGAAAATG AAGGTGATCA TGCAATAACA GTAGCAGACT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTGGTATG GTATTTCAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA CTGTAACAAG TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGCA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAGTT ACATCAGGAT 
CCGGGACAGC CCTTGTACAT TCTCTGGAAT GCACACACCC AAAAATATCC AATGGTCCAT 
TTATTGCAAA AAAGTGATAA CTCATTTAAC CAGGAACTGT TGAAAAGTAT GGTAAAAAGC 
ATTAAAATGA ATGATGTCTA TGGACCAATG AGTCAGATTT TAGAGACACT GAATAAGTGT 
CCACATTTTA AAAGACAGAG GAGTTTATAC AGAGAAATAC TATTTCTCTC ACTTGTGGCA 
CTGGGAAGAG AAAACATTGA TATAGATGCT TTTGATAAAG AGTACAAGAT GGCATACGAT 
CGTCTCACAC CTAGTCAAGT CAAGAGTACA CACAACTGTG ATAGACCACC AAGTACAGGG 
GTGATGGAAT GTCGAAAAAC CTTTGGAGAA CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254179.1
CDS	2519..6040
Translation	MFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005254122.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254122.5

ORF Insert Sequence:

ATGTTCAGAA GAACAAAACA GGAAATTAAA TCAGCCCATA AAATTGCAAA GAGGTACTCT 
TCCATCCCTC AGATGTGGTC TAGGTGTCTA CTGCGCCACT GTTATGGACT GTGGTTTATT 
TGTCTCCCAG CTTATGTGAA AGTCTGTCAT TCAAAAGTCA GGGCTCTGAA AACAGCATAT 
GATGTGCTTA AAAAAATGCA GTCAAAGAAG ATGGATCCAC CTGATGAGGT ATGCTACCGC 
ATTCTTATGC AACTCTGTGG ACAATATGAT CAGCCAGTGC TTGCAGTTCG AGTGCTTTTT 
GAAATGCAGA AAGCTGGTAT TGACCCCAAT GCCATTACTT ATGGTTATTA TAATAAGGCT 
GTTTTGGAAA GTACCTGGCC TTCAAGAAGT CGTAGTGGCT ATTTTCTTTG GACAAAAGTA 
AGAAATGTTG TTTTAGGAGT AACACAGTTC AAAAGAGCTT TAAAGAAGCA TGCACACTTA 
TCACAAACAA CTCTCTCAGC AGATGGCAGT GACCTGGATG CTGTTAGTCA TGGCAGCATG 
GATAGTGGTC ATGGGACACA CACTGTGGAG CAGGCACCTT TTAATACGGG TTTAATCAAA 
GTATATGCTA CTGATGATAG ATCTAGTACA GGTGGCCAGT CTGATCTTGG ATATAATTCA 
TTATCTAAAG ATGAAGTTAG AAGAGGGGAT ACATCTACTG AAGACATTCA AGAAGAAAAA 
GATAAAAAAG GGAGTGATTG TAGTTCCTTG TCAGAGAGTG AGAGTACAAA AGGAAGTGCT 
GATTGCCTTC CTAAGCTCAG TTATCAAAAT TCTTCCAGTA TCGTTCGCCT CACTGGTACA 
AGTAACAACA GTGCTGGTAA AATATCAGGA GAAAGCATGG AAAGCACACC TGAGCTGCTC 
TTAATATCAT CTCTTGAAGA TACAAATGAA ACACGAAACA TTCAAAGTAG ATGCTTCAGG 
AAAAGACATA AAAGTGACAA TGAAACTAAT TTGCAGCAGC AAGTGGTCTG GGGAAATAGA 
AACCGTAATC TTAGTGGAGG GGTACTGATG GGATTTATGC TCAATAGAAT TAACCAGGAA 
GCAACCCCTG GAGATATAGT TGAAAAATTG GGAGCTGATG CAAAAATTCT TTCAAATGTT 
ATCTCAAAAA GCACGAGACC AAATACTCTT GATATTGGGA AGCCACCTTT AAGATCAAAA 
AGAGACAGTC TAGAAAAGGA ATCTAGTGAT GATGATACAC CTTTTGATGG TTCTAACTAT 
TTGGCAGATA AAGTGGATTC TCCTGTTATT TTTGACTTAG AAGACTTAGA CAGTGAAACA 
GATGTATCAA AAGCAGGATG TGTTGCTACA CAAAATCCCA AGAGGATTCA ACGTATGAAC 
AGCAGCTTTT CAGTAAAACC TTTTGAAAAA ACTGATGTCG CAACAGGATT TGATCCCCTC 
TCTCTTTTGG TTGCTGAGAC TGAACAGCAG CAAAAAGAAG AAGAGGAGGA GGATGAAGAT 
GATAGCAAAA GCATTTCAAC ACCATCTGCT AGGCGTGATT TAGCTGAAGA AATTGTGATG 
TATATGAATA ACATGAGCAG TCCTTTGACA AGTCGTACAC CAAGCATTGA TTTACAACGG 
GCATGTGATG ATAAATTAAA TAAGAAAAGT CCACCATTGG TCAAGGCATG CAGAAGATCT 
AGTCTGCCTC CTAATTCTCC TAAGCCAGTG AGACTTACCA AATCTAAAAG TTACACAAAA 
AGTGAGGAGA AACCAAGGGA TAGACTTTGG TCTTCTCCAG CCTTCTCACC TACTTGCCCA 
TTTAGGGAAG AATCTCAGGA TACATTAACC CACTCATCAC CTTCATTTAA CTTAGATACA 
CTACTAGTAC CTAAACTAGA TGTTCTAAGA AACAGTATGT TCACTGCTGG AAAAGGAGTT 
GCAGAGAAAG CAAGCAAATG GTATTCAAGA TTCACCATGT ACACCACATC ATCTAAGGAT 
CAAAGTTCTG ATCGTACCAG TCTTTCTTCA GTGGGAGCCC AGGATTCTGA ATCTACCTCT 
TTAACAGATG AAGATGTCTG CCATGAGTTG GAAGGACCTA TCTCCTCTCA AGAGACCAGT 
GCTACTTCAG GGACTAAGAG AATTGATCTC AGCCGAATAA GCCTGGAAAG TTCTGCATCC 
TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC 
AACGCGAGTA ATACAAATAT CTTCCAGAAC TATGCAATGG AGGTTCTCAT CTCAAGTTGC 
TCTCGGTGTA GAACTTGTGA TTGTCTTGTC CATGATGAGG AAATCATGGC TGGCTGGACA 
GCAGATGATT CAAATCTCAA TACTACATGC CCATTCTGTG GCAATATCTT CTTACCCTTT 
CTGAATATAG AAATAAGAGA TTTAAGACGA CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA 
TCAACAGAAA ATATGCACTT TCCATCCTCC ATTTCAAGTC AGACGAGGCA GTCTTGCATT 
TCAACATCAG CCTCTGGTCT TGACACATCT GCTCTCTCTG TTCAAGGGAA TTTTGATCTA 
AATAGCAAAT CTAAACTGCA GGAAAATTTT TGCACCCGAA GTATTCAGAT CCCTGCTAAT 
AGATCAAAAA CAGCTATGTC TAAATGTCCA ATATTTCCAA TGGCCAGGAG TATTAGTACT 
TCTGGCCCCT TGGATAAGGA AGATACTGGA AGACAGAAGC TCATTTCTAC AGGCAGCCTG 
CCAGCTACTT TACAAGGAGC TACAGATTCT TTAGGTTTAG AATGGCACCT TCCAAGTCCC 
GATCCTGTCA CTGTTCCGTA TCTTAGTCCT TTAGTGGTAT GGAAAGAACT TGAAAGCTTA 
TTGGAAAATG AAGGTGATCA TGCAATAACA GTAGCAGACT TTGTGGACCA TCATCCAATT 
GTTTTTTGGA ACCTGGTATG GTATTTCAGA CGTCTTGACT TGCCCAGTAA CTTACCTGGA 
TTGATTCTTT CTTCTGAGCA CTGTAACAAG TATTCAAAGA TTCCCCGCCA CTGTATGTCT 
GAAGATAGCA AATATGTTTT AATACAAATG TTATGGGACA ATATGAAGTT ACATCAGGAT 
CCGGGACAGC CCTTGTACAT TCTCTGGAAT GCACACACCC AAAAATATCC AATGGTCCAT 
TTATTGCAAA AAAGTGATAA CTCATTTAAC CAGGAACTGT TGAAAAGTAT GGTAAAAAGC 
ATTAAAATGA ATGATGTCTA TGGACCAATG AGTCAGATTT TAGAGACACT GAATAAGTGT 
CCACATTTTA AAAGACAGAG GAGTTTATAC AGAGAAATAC TATTTCTCTC ACTTGTGGCA 
CTGGGAAGAG AAAACATTGA TATAGATGCT TTTGATAAAG AGTACAAGAT GGCATACGAT 
CGTCTCACAC CTAGTCAAGT CAAGAGTACA CACAACTGTG ATAGACCACC AAGTACAGGG 
GTGATGGAAT GTCGAAAAAC CTTTGGAGAA CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( NM_001376920.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363849.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 5, mRNA.

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CloneID	OHu22718
Clone ID Related Accession (Same CDS sequence)	XM_005254121.4 , NM_001376920.1 , NM_005848.4 , XM_005254121.3 , NM_001376919.1 , XM_017021863.1 , XM_017021863.2
Accession Version	NM_001376920.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011939.9. On Nov 27, 2019 this sequence version replaced XM_017021863.2. ##Evidence-Data-START## CDS exon combination :: AL832602.2 [ECO:0000331] ##Evidence-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363849.1
CDS	307..5898
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	NM_001376920.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376920.1

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( NM_001376919.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363848.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 4, mRNA.

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CloneID	OHu22718
Clone ID Related Accession (Same CDS sequence)	XM_005254121.4 , NM_001376920.1 , NM_005848.4 , XM_005254121.3 , NM_001376919.1 , XM_017021863.1 , XM_017021863.2
Accession Version	NM_001376919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011939.9. On Nov 27, 2019 this sequence version replaced XM_005254121.4. ##Evidence-Data-START## CDS exon combination :: AL832602.2 [ECO:0000331] ##Evidence-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363848.1
CDS	386..5977
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	NM_001376919.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376919.1

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_017021864.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877353.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

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CloneID	OHu85227
Clone ID Related Accession (Same CDS sequence)	XM_017021864.2 , XM_017021864.1
Accession Version	XM_017021864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAG TCAAAGTCGT ACTCTTTTGT TTGAGAGTGA GTGTTTGATT 
TTTTTAGTGC TACCTTCTAT GGAGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877353.1
CDS	341..5770
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQSRTLLFESECLIFLVLPSMEV

Target ORF information:

RefSeq Version	XM_017021864.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021864.2

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAG TCAAAGTCGT ACTCTTTTGT TTGAGAGTGA GTGTTTGATT 
TTTTTAGTGC TACCTTCTAT GGAGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_017021864.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877353.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

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CloneID	OHu85227
Clone ID Related Accession (Same CDS sequence)	XM_017021864.2 , XM_017021864.1
Accession Version	XM_017021864.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAG TCAAAGTCGT ACTCTTTTGT TTGAGAGTGA GTGTTTGATT 
TTTTTAGTGC TACCTTCTAT GGAGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877353.1
CDS	381..5810
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHSQSRTLLFESECLIFLVLPSMEV

Target ORF information:

RefSeq Version	XM_017021864.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021864.1

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAG TCAAAGTCGT ACTCTTTTGT TTGAGAGTGA GTGTTTGATT 
TTTTTAGTGC TACCTTCTAT GGAGGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( NM_001144823.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138295.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 1, mRNA.

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CloneID	OHu22448
Clone ID Related Accession (Same CDS sequence)	NM_001144823.2
Accession Version	NM_001144823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK307411.1, AL833317.1, BC028057.1, BC041706.1 and AC011939.9. On Mar 9, 2016 this sequence version replaced NM_001144823.1. Transcript Variant: This variant (1) encodes isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AL833317.1, SRR1803612.46104.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GATGGCAGTG ACCTGGATGC TGTTAGTCAT GGCAGCATGG ATAGTGGTCA TGGGACACAC 
ACTGTGGAGC AGGCACCTTT TAATACGGGT TTAATCAAAG TATATGCTAC TGATGATAGA 
TCTAGTACAG GTGGCCAGTC TGATCTTGGA TATAATTCAT TATCTAAAGA TGAAGTTAGA 
AGAGGGGATA CATCTACTGA AGACATTCAA GAAGAAAAAG ATAAAAAAGG GAGTGATTGT 
AGTTCCTTGT CAGAGAGTGA GAGTACAAAA GGAAGTGCTG ATTGCCTTCC TAAGCTCAGT 
TATCAAAATT CTTCCAGTAT CGTTCGCCTC ACTGGTACAA GTAACAACAG TGCTGGTAAA 
ATATCAGGAG AAAGCATGGA AAGCACACCT GAGCTGCTCT TAATATCATC TCTTGAAGAT 
ACAAATGAAA CACGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAAACTAATT TGCAGCAGCA AGTGGTCTGG GGAAATAGAA ACCGTAATCT TAGTGGAGGG 
GTACTGATGG GATTTATGCT CAATAGAATT AACCAGGAAG CAACCCCTGG AGATATAGTT 
GAAAAATTGG GAGCTGATGC AAAAATTCTT TCAAATGTTA TCTCAAAAAG CACGAGACCA 
AATACTCTTG ATATTGGGAA GCCACCTTTA AGATCAAAAA GAGACAGTCT AGAAAAGGAA 
TCTAGTGATG ATGATACACC TTTTGATGGT TCTAACTATT TGGCAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AGTGAAACAG ATGTATCAAA AGCAGGATGT 
GTTGCTACAC AAAATCCCAA GAGGATTCAA CGTATGAACA GCAGCTTTTC AGTAAAACCT 
TTTGAAAAAA CTGATGTCGC AACAGGATTT GATCCCCTCT CTCTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAAGA AGAGGAGGAG GATGAAGATG ATAGCAAAAG CATTTCAACA 
CCATCTGCTA GGCGTGATTT AGCTGAAGAA ATTGTGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGTACACC AAGCATTGAT TTACAACGGG CATGTGATGA TAAATTAAAT 
AAGAAAAGTC CACCATTGGT CAAGGCATGC AGAAGATCTA GTCTGCCTCC TAATTCTCCT 
AAGCCAGTGA GACTTACCAA ATCTAAAAGT TACACAAAAA GTGAGGAGAA ACCAAGGGAT 
AGACTTTGGT CTTCTCCAGC CTTCTCACCT ACTTGCCCAT TTAGGGAAGA ATCTCAGGAT 
ACATTAACCC ACTCATCACC TTCATTTAAC TTAGATACAC TACTAGTACC TAAACTAGAT 
GTTCTAAGAA ACAGTATGTT CACTGCTGGA AAAGGAGTTG CAGAGAAAGC AAGCAAATGG 
TATTCAAGAT TCACCATGTA CACCACATCA TCTAAGGATC AAAGTTCTGA TCGTACCAGT 
CTTTCTTCAG TGGGAGCCCA GGATTCTGAA TCTACCTCTT TAACAGATGA AGATGTCTGC 
CATGAGTTGG AAGGACCTAT CTCCTCTCAA GAGACCAGTG CTACTTCAGG GACTAAGAGA 
ATTGATCTCA GCCGAATAAG CCTGGAAAGT TCTGCATCCT TGGAAGGATC TCTGTCGAAG 
TTTGCCTTAC CTGGGAAATC AGAAGTGACA TCTTCCTTCA ACGCGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTTCTCATC TCAAGTTGCT CTCGGTGTAG AACTTGTGAT 
TGTCTTGTCC ATGATGAGGA AATCATGGCT GGCTGGACAG CAGATGATTC AAATCTCAAT 
ACTACATGCC CATTCTGTGG CAATATCTTC TTACCCTTTC TGAATATAGA AATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTCTAAAG TCAAGCCCAT CAACAGAAAA TATGCACTTT 
CCATCCTCCA TTTCAAGTCA GACGAGGCAG TCTTGCATTT CAACATCAGC CTCTGGTCTT 
GACACATCTG CTCTCTCTGT TCAAGGGAAT TTTGATCTAA ATAGCAAATC TAAACTGCAG 
GAAAATTTTT GCACCCGAAG TATTCAGATC CCTGCTAATA GATCAAAAAC AGCTATGTCT 
AAATGTCCAA TATTTCCAAT GGCCAGGAGT ATTAGTACTT CTGGCCCCTT GGATAAGGAA 
GATACTGGAA GACAGAAGCT CATTTCTACA GGCAGCCTGC CAGCTACTTT ACAAGGAGCT 
ACAGATTCTT TAGGTTTAGA ATGGCACCTT CCAAGTCCCG ATCCTGTCAC TGTTCCGTAT 
CTTAGTCCTT TAGTGGTATG GAAAGAACTT GAAAGCTTAT TGGAAAATGA AGGTGATCAT 
GCAATAACAG TAGCAGACTT TGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTATGG 
TATTTCAGAC GTCTTGACTT GCCCAGTAAC TTACCTGGAT TGATTCTTTC TTCTGAGCAC 
TGTAACAAGT ATTCAAAGAT TCCCCGCCAC TGTATGTCTG AAGATAGCAA ATATGTTTTA 
ATACAAATGT TATGGGACAA TATGAAGTTA CATCAGGATC CGGGACAGCC CTTGTACATT 
CTCTGGAATG CACACACCCA AAAATATCCA ATGGTCCATT TATTGCAAAA AAGTGATAAC 
TCATTTAACC AGGAACTGTT GAAAAGTATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGAGACACTG AATAAGTGTC CACATTTTAA AAGACAGAGG 
AGTTTATACA GAGAAATACT ATTTCTCTCA CTTGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCTT TTGATAAAGA GTACAAGATG GCATACGATC GTCTCACACC TAGTCAAGTC 
AAGAGTACAC ACAACTGTGA TAGACCACCA AGTACAGGGG TGATGGAATG TCGAAAAACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138295.1
CDS	386..6106
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSDGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	NM_001144823.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144823.2

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GATGGCAGTG ACCTGGATGC TGTTAGTCAT GGCAGCATGG ATAGTGGTCA TGGGACACAC 
ACTGTGGAGC AGGCACCTTT TAATACGGGT TTAATCAAAG TATATGCTAC TGATGATAGA 
TCTAGTACAG GTGGCCAGTC TGATCTTGGA TATAATTCAT TATCTAAAGA TGAAGTTAGA 
AGAGGGGATA CATCTACTGA AGACATTCAA GAAGAAAAAG ATAAAAAAGG GAGTGATTGT 
AGTTCCTTGT CAGAGAGTGA GAGTACAAAA GGAAGTGCTG ATTGCCTTCC TAAGCTCAGT 
TATCAAAATT CTTCCAGTAT CGTTCGCCTC ACTGGTACAA GTAACAACAG TGCTGGTAAA 
ATATCAGGAG AAAGCATGGA AAGCACACCT GAGCTGCTCT TAATATCATC TCTTGAAGAT 
ACAAATGAAA CACGAAACAT TCAAAGTAGA TGCTTCAGGA AAAGACATAA AAGTGACAAT 
GAAACTAATT TGCAGCAGCA AGTGGTCTGG GGAAATAGAA ACCGTAATCT TAGTGGAGGG 
GTACTGATGG GATTTATGCT CAATAGAATT AACCAGGAAG CAACCCCTGG AGATATAGTT 
GAAAAATTGG GAGCTGATGC AAAAATTCTT TCAAATGTTA TCTCAAAAAG CACGAGACCA 
AATACTCTTG ATATTGGGAA GCCACCTTTA AGATCAAAAA GAGACAGTCT AGAAAAGGAA 
TCTAGTGATG ATGATACACC TTTTGATGGT TCTAACTATT TGGCAGATAA AGTGGATTCT 
CCTGTTATTT TTGACTTAGA AGACTTAGAC AGTGAAACAG ATGTATCAAA AGCAGGATGT 
GTTGCTACAC AAAATCCCAA GAGGATTCAA CGTATGAACA GCAGCTTTTC AGTAAAACCT 
TTTGAAAAAA CTGATGTCGC AACAGGATTT GATCCCCTCT CTCTTTTGGT TGCTGAGACT 
GAACAGCAGC AAAAAGAAGA AGAGGAGGAG GATGAAGATG ATAGCAAAAG CATTTCAACA 
CCATCTGCTA GGCGTGATTT AGCTGAAGAA ATTGTGATGT ATATGAATAA CATGAGCAGT 
CCTTTGACAA GTCGTACACC AAGCATTGAT TTACAACGGG CATGTGATGA TAAATTAAAT 
AAGAAAAGTC CACCATTGGT CAAGGCATGC AGAAGATCTA GTCTGCCTCC TAATTCTCCT 
AAGCCAGTGA GACTTACCAA ATCTAAAAGT TACACAAAAA GTGAGGAGAA ACCAAGGGAT 
AGACTTTGGT CTTCTCCAGC CTTCTCACCT ACTTGCCCAT TTAGGGAAGA ATCTCAGGAT 
ACATTAACCC ACTCATCACC TTCATTTAAC TTAGATACAC TACTAGTACC TAAACTAGAT 
GTTCTAAGAA ACAGTATGTT CACTGCTGGA AAAGGAGTTG CAGAGAAAGC AAGCAAATGG 
TATTCAAGAT TCACCATGTA CACCACATCA TCTAAGGATC AAAGTTCTGA TCGTACCAGT 
CTTTCTTCAG TGGGAGCCCA GGATTCTGAA TCTACCTCTT TAACAGATGA AGATGTCTGC 
CATGAGTTGG AAGGACCTAT CTCCTCTCAA GAGACCAGTG CTACTTCAGG GACTAAGAGA 
ATTGATCTCA GCCGAATAAG CCTGGAAAGT TCTGCATCCT TGGAAGGATC TCTGTCGAAG 
TTTGCCTTAC CTGGGAAATC AGAAGTGACA TCTTCCTTCA ACGCGAGTAA TACAAATATC 
TTCCAGAACT ATGCAATGGA GGTTCTCATC TCAAGTTGCT CTCGGTGTAG AACTTGTGAT 
TGTCTTGTCC ATGATGAGGA AATCATGGCT GGCTGGACAG CAGATGATTC AAATCTCAAT 
ACTACATGCC CATTCTGTGG CAATATCTTC TTACCCTTTC TGAATATAGA AATAAGAGAT 
TTAAGACGAC CTGGAAGATA CTTTCTAAAG TCAAGCCCAT CAACAGAAAA TATGCACTTT 
CCATCCTCCA TTTCAAGTCA GACGAGGCAG TCTTGCATTT CAACATCAGC CTCTGGTCTT 
GACACATCTG CTCTCTCTGT TCAAGGGAAT TTTGATCTAA ATAGCAAATC TAAACTGCAG 
GAAAATTTTT GCACCCGAAG TATTCAGATC CCTGCTAATA GATCAAAAAC AGCTATGTCT 
AAATGTCCAA TATTTCCAAT GGCCAGGAGT ATTAGTACTT CTGGCCCCTT GGATAAGGAA 
GATACTGGAA GACAGAAGCT CATTTCTACA GGCAGCCTGC CAGCTACTTT ACAAGGAGCT 
ACAGATTCTT TAGGTTTAGA ATGGCACCTT CCAAGTCCCG ATCCTGTCAC TGTTCCGTAT 
CTTAGTCCTT TAGTGGTATG GAAAGAACTT GAAAGCTTAT TGGAAAATGA AGGTGATCAT 
GCAATAACAG TAGCAGACTT TGTGGACCAT CATCCAATTG TTTTTTGGAA CCTGGTATGG 
TATTTCAGAC GTCTTGACTT GCCCAGTAAC TTACCTGGAT TGATTCTTTC TTCTGAGCAC 
TGTAACAAGT ATTCAAAGAT TCCCCGCCAC TGTATGTCTG AAGATAGCAA ATATGTTTTA 
ATACAAATGT TATGGGACAA TATGAAGTTA CATCAGGATC CGGGACAGCC CTTGTACATT 
CTCTGGAATG CACACACCCA AAAATATCCA ATGGTCCATT TATTGCAAAA AAGTGATAAC 
TCATTTAACC AGGAACTGTT GAAAAGTATG GTAAAAAGCA TTAAAATGAA TGATGTCTAT 
GGACCAATGA GTCAGATTTT AGAGACACTG AATAAGTGTC CACATTTTAA AAGACAGAGG 
AGTTTATACA GAGAAATACT ATTTCTCTCA CTTGTGGCAC TGGGAAGAGA AAACATTGAT 
ATAGATGCTT TTGATAAAGA GTACAAGATG GCATACGATC GTCTCACACC TAGTCAAGTC 
AAGAGTACAC ACAACTGTGA TAGACCACCA AGTACAGGGG TGATGGAATG TCGAAAAACC 
TTTGGAGAAC CTTATCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( NM_005848.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005839.3 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 2, mRNA.

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CloneID	OHu22718
Clone ID Related Accession (Same CDS sequence)	XM_005254121.4 , NM_001376920.1 , NM_005848.4 , XM_005254121.3 , NM_001376919.1 , XM_017021863.1 , XM_017021863.2
Accession Version	NM_005848.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB462436.1, BC041706.1 and AC011939.9. On Mar 9, 2016 this sequence version replaced NM_005848.3. Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC041706.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005839.3
CDS	379..5970
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	NM_005848.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005848.4

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( NM_001320835.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001307764.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 3, mRNA.

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CloneID	OHu48054
Clone ID Related Accession (Same CDS sequence)	XM_005254120.4 , XM_017021862.1 , XM_005254120.5 , NM_001320835.1 , XM_006720371.4 , XM_017021862.2 , XM_006720371.3
Accession Version	NM_001320835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-29
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011939.9. On Mar 9, 2016 this sequence version replaced XM_005254119.2. Transcript Variant: This variant (3) uses an alternate splice site compared to variant 1. The encoded isoform (3) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.75482.1, SRR1803615.173183.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001307764.1
CDS	386..6109
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	NM_001320835.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001320835.1

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005254121.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254178.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

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CloneID	OHu22718
Clone ID Related Accession (Same CDS sequence)	XM_005254121.4 , NM_001376920.1 , NM_005848.4 , XM_005254121.3 , NM_001376919.1 , XM_017021863.1 , XM_017021863.2
Accession Version	XM_005254121.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005254121.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254178.1
CDS	341..5932
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005254121.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254121.4

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005254121.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254178.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

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CloneID	OHu22718
Clone ID Related Accession (Same CDS sequence)	XM_005254121.4 , NM_001376920.1 , NM_005848.4 , XM_005254121.3 , NM_001376919.1 , XM_017021863.1 , XM_017021863.2
Accession Version	XM_005254121.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254178.1
CDS	386..5977
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005254121.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254121.3

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_017021863.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877352.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

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CloneID	OHu22718
Clone ID Related Accession (Same CDS sequence)	XM_005254121.4 , NM_001376920.1 , NM_005848.4 , XM_005254121.3 , NM_001376919.1 , XM_017021863.1 , XM_017021863.2
Accession Version	XM_017021863.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877352.1
CDS	164..5755
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_017021863.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021863.1

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_017021863.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877352.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

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CloneID	OHu22718
Clone ID Related Accession (Same CDS sequence)	XM_005254121.4 , NM_001376920.1 , NM_005848.4 , XM_005254121.3 , NM_001376919.1 , XM_017021863.1 , XM_017021863.2
Accession Version	XM_017021863.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021863.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877352.1
CDS	164..5755
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_017021863.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021863.2

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GGTGGCCAGT CTGATCTTGG ATATAATTCA TTATCTAAAG ATGAAGTTAG AAGAGGGGAT 
ACATCTACTG AAGACATTCA AGAAGAAAAA GATAAAAAAG GGAGTGATTG TAGTTCCTTG 
TCAGAGAGTG AGAGTACAAA AGGAAGTGCT GATTGCCTTC CTAAGCTCAG TTATCAAAAT 
TCTTCCAGTA TCGTTCGCCT CACTGGTACA AGTAACAACA GTGCTGGTAA AATATCAGGA 
GAAAGCATGG AAAGCACACC TGAGCTGCTC TTAATATCAT CTCTTGAAGA TACAAATGAA 
ACACGAAACA TTCAAAGTAG ATGCTTCAGG AAAAGACATA AAAGTGACAA TGAAACTAAT 
TTGCAGCAGC AAGTGGTCTG GGGAAATAGA AACCGTAATC TTAGTGGAGG GGTACTGATG 
GGATTTATGC TCAATAGAAT TAACCAGGAA GCAACCCCTG GAGATATAGT TGAAAAATTG 
GGAGCTGATG CAAAAATTCT TTCAAATGTT ATCTCAAAAA GCACGAGACC AAATACTCTT 
GATATTGGGA AGCCACCTTT AAGATCAAAA AGAGACAGTC TAGAAAAGGA ATCTAGTGAT 
GATGATACAC CTTTTGATGG TTCTAACTAT TTGGCAGATA AAGTGGATTC TCCTGTTATT 
TTTGACTTAG AAGACTTAGA CAGTGAAACA GATGTATCAA AAGCAGGATG TGTTGCTACA 
CAAAATCCCA AGAGGATTCA ACGTATGAAC AGCAGCTTTT CAGTAAAACC TTTTGAAAAA 
ACTGATGTCG CAACAGGATT TGATCCCCTC TCTCTTTTGG TTGCTGAGAC TGAACAGCAG 
CAAAAAGAAG AAGAGGAGGA GGATGAAGAT GATAGCAAAA GCATTTCAAC ACCATCTGCT 
AGGCGTGATT TAGCTGAAGA AATTGTGATG TATATGAATA ACATGAGCAG TCCTTTGACA 
AGTCGTACAC CAAGCATTGA TTTACAACGG GCATGTGATG ATAAATTAAA TAAGAAAAGT 
CCACCATTGG TCAAGGCATG CAGAAGATCT AGTCTGCCTC CTAATTCTCC TAAGCCAGTG 
AGACTTACCA AATCTAAAAG TTACACAAAA AGTGAGGAGA AACCAAGGGA TAGACTTTGG 
TCTTCTCCAG CCTTCTCACC TACTTGCCCA TTTAGGGAAG AATCTCAGGA TACATTAACC 
CACTCATCAC CTTCATTTAA CTTAGATACA CTACTAGTAC CTAAACTAGA TGTTCTAAGA 
AACAGTATGT TCACTGCTGG AAAAGGAGTT GCAGAGAAAG CAAGCAAATG GTATTCAAGA 
TTCACCATGT ACACCACATC ATCTAAGGAT CAAAGTTCTG ATCGTACCAG TCTTTCTTCA 
GTGGGAGCCC AGGATTCTGA ATCTACCTCT TTAACAGATG AAGATGTCTG CCATGAGTTG 
GAAGGACCTA TCTCCTCTCA AGAGACCAGT GCTACTTCAG GGACTAAGAG AATTGATCTC 
AGCCGAATAA GCCTGGAAAG TTCTGCATCC TTGGAAGGAT CTCTGTCGAA GTTTGCCTTA 
CCTGGGAAAT CAGAAGTGAC ATCTTCCTTC AACGCGAGTA ATACAAATAT CTTCCAGAAC 
TATGCAATGG AGGTTCTCAT CTCAAGTTGC TCTCGGTGTA GAACTTGTGA TTGTCTTGTC 
CATGATGAGG AAATCATGGC TGGCTGGACA GCAGATGATT CAAATCTCAA TACTACATGC 
CCATTCTGTG GCAATATCTT CTTACCCTTT CTGAATATAG AAATAAGAGA TTTAAGACGA 
CCTGGAAGAT ACTTTCTAAA GTCAAGCCCA TCAACAGAAA ATATGCACTT TCCATCCTCC 
ATTTCAAGTC AGACGAGGCA GTCTTGCATT TCAACATCAG CCTCTGGTCT TGACACATCT 
GCTCTCTCTG TTCAAGGGAA TTTTGATCTA AATAGCAAAT CTAAACTGCA GGAAAATTTT 
TGCACCCGAA GTATTCAGAT CCCTGCTAAT AGATCAAAAA CAGCTATGTC TAAATGTCCA 
ATATTTCCAA TGGCCAGGAG TATTAGTACT TCTGGCCCCT TGGATAAGGA AGATACTGGA 
AGACAGAAGC TCATTTCTAC AGGCAGCCTG CCAGCTACTT TACAAGGAGC TACAGATTCT 
TTAGGTTTAG AATGGCACCT TCCAAGTCCC GATCCTGTCA CTGTTCCGTA TCTTAGTCCT 
TTAGTGGTAT GGAAAGAACT TGAAAGCTTA TTGGAAAATG AAGGTGATCA TGCAATAACA 
GTAGCAGACT TTGTGGACCA TCATCCAATT GTTTTTTGGA ACCTGGTATG GTATTTCAGA 
CGTCTTGACT TGCCCAGTAA CTTACCTGGA TTGATTCTTT CTTCTGAGCA CTGTAACAAG 
TATTCAAAGA TTCCCCGCCA CTGTATGTCT GAAGATAGCA AATATGTTTT AATACAAATG 
TTATGGGACA ATATGAAGTT ACATCAGGAT CCGGGACAGC CCTTGTACAT TCTCTGGAAT 
GCACACACCC AAAAATATCC AATGGTCCAT TTATTGCAAA AAAGTGATAA CTCATTTAAC 
CAGGAACTGT TGAAAAGTAT GGTAAAAAGC ATTAAAATGA ATGATGTCTA TGGACCAATG 
AGTCAGATTT TAGAGACACT GAATAAGTGT CCACATTTTA AAAGACAGAG GAGTTTATAC 
AGAGAAATAC TATTTCTCTC ACTTGTGGCA CTGGGAAGAG AAAACATTGA TATAGATGCT 
TTTGATAAAG AGTACAAGAT GGCATACGAT CGTCTCACAC CTAGTCAAGT CAAGAGTACA 
CACAACTGTG ATAGACCACC AAGTACAGGG GTGATGGAAT GTCGAAAAAC CTTTGGAGAA 
CCTTATCTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005254120.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254177.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

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CloneID	OHu48054
Clone ID Related Accession (Same CDS sequence)	XM_005254120.4 , XM_017021862.1 , XM_005254120.5 , NM_001320835.1 , XM_006720371.4 , XM_017021862.2 , XM_006720371.3
Accession Version	XM_005254120.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254120.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254177.1
CDS	165..5888
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005254120.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254120.4

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_017021862.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877351.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

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CloneID	OHu48054
Clone ID Related Accession (Same CDS sequence)	XM_005254120.4 , XM_017021862.1 , XM_005254120.5 , NM_001320835.1 , XM_006720371.4 , XM_017021862.2 , XM_006720371.3
Accession Version	XM_017021862.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877351.1
CDS	106..5829
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_017021862.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021862.1

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_005254120.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254177.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

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CloneID	OHu48054
Clone ID Related Accession (Same CDS sequence)	XM_005254120.4 , XM_017021862.1 , XM_005254120.5 , NM_001320835.1 , XM_006720371.4 , XM_017021862.2 , XM_006720371.3
Accession Version	XM_005254120.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005254120.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254177.1
CDS	164..5887
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_005254120.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254120.5

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_006720371.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720434.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

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CloneID	OHu48054
Clone ID Related Accession (Same CDS sequence)	XM_005254120.4 , XM_017021862.1 , XM_005254120.5 , NM_001320835.1 , XM_006720371.4 , XM_017021862.2 , XM_006720371.3
Accession Version	XM_006720371.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006720371.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720434.1
CDS	121..5844
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_006720371.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720371.4

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_017021862.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877351.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

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CloneID	OHu48054
Clone ID Related Accession (Same CDS sequence)	XM_005254120.4 , XM_017021862.1 , XM_005254120.5 , NM_001320835.1 , XM_006720371.4 , XM_017021862.2 , XM_006720371.3
Accession Version	XM_017021862.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017021862.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877351.1
CDS	106..5829
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_017021862.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021862.2

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_006720371.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720434.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

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CloneID	OHu48054
Clone ID Related Accession (Same CDS sequence)	XM_005254120.4 , XM_017021862.1 , XM_005254120.5 , NM_001320835.1 , XM_006720371.4 , XM_017021862.2 , XM_006720371.3
Accession Version	XM_006720371.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006720371.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720434.1
CDS	121..5844
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_006720371.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720371.3

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGG ATCAAAGTTC TGATCGTACC 
AGTCTTTCTT CAGTGGGAGC CCAGGATTCT GAATCTACCT CTTTAACAGA TGAAGATGTC 
TGCCATGAGT TGGAAGGACC TATCTCCTCT CAAGAGACCA GTGCTACTTC AGGGACTAAG 
AGAATTGATC TCAGCCGAAT AAGCCTGGAA AGTTCTGCAT CCTTGGAAGG ATCTCTGTCG 
AAGTTTGCCT TACCTGGGAA ATCAGAAGTG ACATCTTCCT TCAACGCGAG TAATACAAAT 
ATCTTCCAGA ACTATGCAAT GGAGGTTCTC ATCTCAAGTT GCTCTCGGTG TAGAACTTGT 
GATTGTCTTG TCCATGATGA GGAAATCATG GCTGGCTGGA CAGCAGATGA TTCAAATCTC 
AATACTACAT GCCCATTCTG TGGCAATATC TTCTTACCCT TTCTGAATAT AGAAATAAGA 
GATTTAAGAC GACCTGGAAG ATACTTTCTA AAGTCAAGCC CATCAACAGA AAATATGCAC 
TTTCCATCCT CCATTTCAAG TCAGACGAGG CAGTCTTGCA TTTCAACATC AGCCTCTGGT 
CTTGACACAT CTGCTCTCTC TGTTCAAGGG AATTTTGATC TAAATAGCAA ATCTAAACTG 
CAGGAAAATT TTTGCACCCG AAGTATTCAG ATCCCTGCTA ATAGATCAAA AACAGCTATG 
TCTAAATGTC CAATATTTCC AATGGCCAGG AGTATTAGTA CTTCTGGCCC CTTGGATAAG 
GAAGATACTG GAAGACAGAA GCTCATTTCT ACAGGCAGCC TGCCAGCTAC TTTACAAGGA 
GCTACAGATT CTTTAGGTTT AGAATGGCAC CTTCCAAGTC CCGATCCTGT CACTGTTCCG 
TATCTTAGTC CTTTAGTGGT ATGGAAAGAA CTTGAAAGCT TATTGGAAAA TGAAGGTGAT 
CATGCAATAA CAGTAGCAGA CTTTGTGGAC CATCATCCAA TTGTTTTTTG GAACCTGGTA 
TGGTATTTCA GACGTCTTGA CTTGCCCAGT AACTTACCTG GATTGATTCT TTCTTCTGAG 
CACTGTAACA AGTATTCAAA GATTCCCCGC CACTGTATGT CTGAAGATAG CAAATATGTT 
TTAATACAAA TGTTATGGGA CAATATGAAG TTACATCAGG ATCCGGGACA GCCCTTGTAC 
ATTCTCTGGA ATGCACACAC CCAAAAATAT CCAATGGTCC ATTTATTGCA AAAAAGTGAT 
AACTCATTTA ACCAGGAACT GTTGAAAAGT ATGGTAAAAA GCATTAAAAT GAATGATGTC 
TATGGACCAA TGAGTCAGAT TTTAGAGACA CTGAATAAGT GTCCACATTT TAAAAGACAG 
AGGAGTTTAT ACAGAGAAAT ACTATTTCTC TCACTTGTGG CACTGGGAAG AGAAAACATT 
GATATAGATG CTTTTGATAA AGAGTACAAG ATGGCATACG ATCGTCTCAC ACCTAGTCAA 
GTCAAGAGTA CACACAACTG TGATAGACCA CCAAGTACAG GGGTGATGGA ATGTCGAAAA 
ACCTTTGGAG AACCTTATCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_011521156.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519458.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.

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CloneID	OHu63822
Clone ID Related Accession (Same CDS sequence)	XM_011521156.3 , XM_011521156.2
Accession Version	XM_011521156.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521156.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519458.1
CDS	341..4522
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSK

Target ORF information:

RefSeq Version	XM_011521156.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521156.3

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_011521156.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519458.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu63822
Clone ID Related Accession (Same CDS sequence)	XM_011521156.3 , XM_011521156.2
Accession Version	XM_011521156.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519458.1
CDS	381..4562
Translation	MIEDKGPRVADYFVVAGLTDVSKPLEEEIHFNDACHKVAKPKEPITDVSVIIKSLGEEVPQDYICIDVTPTGLSADLNNGSLVGPQIYLCYRRGRDKPPLTDLGVLYDWKERLKQGCEIIQSTPYGRPANISGSTSSQRIYITYRRASENMTQNTLAVTDICIIIPSKGESPPHTFCKVDKNLNNSMWGSAVYLCYKKSVAKTNTVSYKAGLICRYPQEDYESFSLPESVPLFCLPMGATIECWPSNSKYPLPVFSTFVLTGASAEKVYGAAIQFYEPYSEENLTEKQRLLLGLTSADGKSDSSKTIHTNKCICLLSHWPFFDAFRKFLTFLYRYSISGPHVLPIEKHISHFMHKVPFPSPQRPRILVQLSPHDNLILSQPVSSPLPLSGGKFSTLLQNLGPENAVTLLVFAVTEHKILIHSLRPSVLTSVTEALVSMIFPFHWPCPYVPLCPLALADVLSAPCPFIVGIDSRYFDLYDPPPDVSCVDVDTNTISQIGDKKNVAWKILPKKPCKNLMNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSK

Target ORF information:

RefSeq Version	XM_011521156.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521156.2

ORF Insert Sequence:

ATGATTGAAG ACAAGGGGCC TCGTGTTGCT GACTACTTTG TTGTAGCAGG ATTAACTGAT 
GTTTCAAAGC CTCTAGAAGA AGAAATTCAC TTCAATGATG CTTGTCATAA AGTAGCTAAA 
CCAAAAGAAC CTATTACAGA TGTTTCAGTT ATTATCAAAT CTCTTGGGGA GGAAGTCCCA 
CAGGATTATA TCTGTATTGA TGTTACCCCA ACTGGATTGT CAGCTGATCT TAATAATGGA 
AGTCTTGTGG GGCCACAGAT TTACCTTTGC TATAGAAGAG GAAGAGATAA GCCTCCACTT 
ACAGATCTGG GGGTTTTATA TGACTGGAAA GAAAGATTGA AACAGGGTTG TGAAATTATT 
CAGAGTACTC CCTATGGGCG CCCCGCAAAT ATTAGTGGGA GTACCTCATC ACAAAGAATT 
TATATCACTT ACCGAAGAGC CTCTGAAAAC ATGACTCAGA ATACGTTGGC TGTCACTGAC 
ATATGTATTA TTATACCCAG TAAAGGAGAA AGCCCACCAC ACACGTTCTG CAAAGTCGAC 
AAGAATCTCA ATAACAGTAT GTGGGGATCA GCAGTATACT TGTGTTATAA AAAATCGGTG 
GCAAAGACGA ACACTGTATC TTACAAAGCT GGCCTAATTT GTAGATATCC TCAAGAAGAT 
TATGAGTCAT TCTCACTACC GGAATCTGTT CCTCTATTTT GTTTACCAAT GGGTGCAACC 
ATTGAATGTT GGCCATCAAA TAGCAAATAC CCTCTGCCTG TATTTTCTAC TTTTGTTTTA 
ACTGGAGCCT CAGCTGAAAA GGTTTATGGT GCTGCTATTC AGTTTTATGA ACCATACTCT 
GAGGAGAATC TCACAGAAAA GCAGAGACTT CTTTTGGGTT TAACATCAGC AGATGGGAAG 
TCTGATAGTT CCAAAACAAT TCATACTAAC AAATGCATCT GTCTTCTTTC TCACTGGCCT 
TTTTTTGATG CATTCAGGAA GTTTCTGACT TTTCTGTATC GTTATTCCAT CTCTGGGCCT 
CATGTCCTTC CAATTGAGAA GCATATTTCT CATTTTATGC ATAAAGTTCC TTTTCCATCT 
CCTCAGAGAC CACGGATTTT AGTTCAGTTA TCACCACATG ATAATCTGAT TCTCAGTCAG 
CCTGTGTCTT CACCTCTTCC ACTAAGTGGT GGCAAGTTTT CAACACTACT GCAGAATTTA 
GGCCCTGAAA ATGCTGTGAC ACTACTGGTG TTTGCAGTAA CAGAACATAA AATTCTTATC 
CATTCCCTAC GGCCATCCGT GCTTACTAGT GTGACAGAAG CATTAGTGTC TATGATTTTC 
CCTTTCCACT GGCCATGCCC GTATGTTCCT CTCTGCCCAC TGGCTTTAGC AGATGTCTTG 
AGTGCACCAT GTCCATTCAT AGTAGGGATT GATTCAAGAT ACTTTGATCT CTATGATCCT 
CCACCAGATG TCAGTTGTGT TGATGTAGAT ACCAACACTA TTTCTCAAAT TGGTGACAAG 
AAAAATGTAG CCTGGAAGAT ACTTCCAAAG AAGCCATGTA AAAATCTGAT GAACACACTG 
AATAATTTGC ATCAGCAATT GGCAAAATTG CAGCAGAGAC CGAGAGATGA TGGACTCATG 
GATTTAGCAA TAAATGACTA TGATTTTAAT TCTGGAAAGA GGTTGCACAT GATAGATTTG 
GAAATCCAAG AGGCATTTTT GTTTTTCATG GCCTCTATTT TAAAAGGTTA CAGATCATAC 
TTAAGGCCAA TAACACAAGC CCCATCTGAG ACAGCCACAG ATGCAGCCTC TCTCTTTGCC 
CTACAAGCTT TCTTAAGAAG CCGGGACCGG TCACATCAAA AATTCTATAA CATGATGACC 
AAAACACAAA TGTTTATTCG CTTCATTGAA GAATGTTCTT TTGTTAGTGA CAAAGATGCA 
AGCCTGGCAT TTTTTGATGA TTGTGTAGAT AAAGTGGATA TGGACAAGAG CGGTGAAGTA 
CGACTTATAG AACTGGATGA ATCTTTCAAA AGTGAACACA CTGTTTTTGT AACACCACCT 
GAGATCCCCC ACCTACCCAA TGGTGAAGAA CCCCCTTTAC AGTACAGCTA TAATGGATTT 
CCAGTTCTTA GGAACAATTT ATTTGAAAGA CCTGAAGGAT TTCTACAAGC AAAGAAGAAC 
AAATTGCCTT CAAAATCAAG TAGTCCTAAT AGCCCTTTGC CCATGTTCAG AAGAACAAAA 
CAGGAAATTA AATCAGCCCA TAAAATTGCA AAGAGGTACT CTTCCATCCC TCAGATGTGG 
TCTAGGTGTC TACTGCGCCA CTGTTATGGA CTGTGGTTTA TTTGTCTCCC AGCTTATGTG 
AAAGTCTGTC ATTCAAAAGT CAGGGCTCTG AAAACAGCAT ATGATGTGCT TAAAAAAATG 
CAGTCAAAGA AGATGGATCC ACCTGATGAG GTATGCTACC GCATTCTTAT GCAACTCTGT 
GGACAATATG ATCAGCCAGT GCTTGCAGTT CGAGTGCTTT TTGAAATGCA GAAAGCTGGT 
ATTGACCCCA ATGCCATTAC TTATGGTTAT TATAATAAGG CTGTTTTGGA AAGTACCTGG 
CCTTCAAGAA GTCGTAGTGG CTATTTTCTT TGGACAAAAG TAAGAAATGT TGTTTTAGGA 
GTAACACAGT TCAAAAGAGC TTTAAAGAAG CATGCACACT TATCACAAAC AACTCTCTCA 
GCAGATGGCA GTGACCTGGA TGCTGTTAGT CATGGCAGCA TGGATAGTGG TCATGGGACA 
CACACTGTGG AGCAGGCACC TTTTAATACG GGTTTAATCA AAGTATATGC TACTGATGAT 
AGATCTAGTA CAGGTGGCCA GTCTGATCTT GGATATAATT CATTATCTAA AGATGAAGTT 
AGAAGAGGGG ATACATCTAC TGAAGACATT CAAGAAGAAA AAGATAAAAA AGGGAGTGAT 
TGTAGTTCCT TGTCAGAGAG TGAGAGTACA AAAGGAAGTG CTGATTGCCT TCCTAAGCTC 
AGTTATCAAA ATTCTTCCAG TATCGTTCGC CTCACTGGTA CAAGTAACAA CAGTGCTGGT 
AAAATATCAG GAGAAAGCAT GGAAAGCACA CCTGAGCTGC TCTTAATATC ATCTCTTGAA 
GATACAAATG AAACACGAAA CATTCAAAGT AGATGCTTCA GGAAAAGACA TAAAAGTGAC 
AATGAAACTA ATTTGCAGCA GCAAGTGGTC TGGGGAAATA GAAACCGTAA TCTTAGTGGA 
GGGGTACTGA TGGGATTTAT GCTCAATAGA ATTAACCAGG AAGCAACCCC TGGAGATATA 
GTTGAAAAAT TGGGAGCTGA TGCAAAAATT CTTTCAAATG TTATCTCAAA AAGCACGAGA 
CCAAATACTC TTGATATTGG GAAGCCACCT TTAAGATCAA AAAGAGACAG TCTAGAAAAG 
GAATCTAGTG ATGATGATAC ACCTTTTGAT GGTTCTAACT ATTTGGCAGA TAAAGTGGAT 
TCTCCTGTTA TTTTTGACTT AGAAGACTTA GACAGTGAAA CAGATGTATC AAAAGCAGGA 
TGTGTTGCTA CACAAAATCC CAAGAGGATT CAACGTATGA ACAGCAGCTT TTCAGTAAAA 
CCTTTTGAAA AAACTGATGT CGCAACAGGA TTTGATCCCC TCTCTCTTTT GGTTGCTGAG 
ACTGAACAGC AGCAAAAAGA AGAAGAGGAG GAGGATGAAG ATGATAGCAA AAGCATTTCA 
ACACCATCTG CTAGGCGTGA TTTAGCTGAA GAAATTGTGA TGTATATGAA TAACATGAGC 
AGTCCTTTGA CAAGTCGTAC ACCAAGCATT GATTTACAAC GGGCATGTGA TGATAAATTA 
AATAAGAAAA GTCCACCATT GGTCAAGGCA TGCAGAAGAT CTAGTCTGCC TCCTAATTCT 
CCTAAGCCAG TGAGACTTAC CAAATCTAAA AGTTACACAA AAAGTGAGGA GAAACCAAGG 
GATAGACTTT GGTCTTCTCC AGCCTTCTCA CCTACTTGCC CATTTAGGGA AGAATCTCAG 
GATACATTAA CCCACTCATC ACCTTCATTT AACTTAGATA CACTACTAGT ACCTAAACTA 
GATGTTCTAA GAAACAGTAT GTTCACTGCT GGAAAAGGAG TTGCAGAGAA AGCAAGCAAA 
TGGTATTCAA GATTCACCAT GTACACCACA TCATCTAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_011521157.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519459.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu63823
Clone ID Related Accession (Same CDS sequence)	XM_011521157.2 , XM_011521157.3
Accession Version	XM_011521157.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAC TGAATAATTT GCATCAGCAA TTGGCAAAAT TGCAGCAGAG ACCGAGAGAT 
GATGGACTCA TGGATTTAGC AATAAATGAC TATGATTTTA ATTCTGGAAA GAGGTTGCAC 
ATGATAGATT TGGAAATCCA AGAGGCATTT TTGTTTTTCA TGGCCTCTAT TTTAAAAGGT 
TACAGATCAT ACTTAAGGCC AATAACACAA GCCCCATCTG AGACAGCCAC AGATGCAGCC 
TCTCTCTTTG CCCTACAAGC TTTCTTAAGA AGCCGGGACC GGTCACATCA AAAATTCTAT 
AACATGATGA CCAAAACACA AATGTTTATT CGCTTCATTG AAGAATGTTC TTTTGTTAGT 
GACAAAGATG CAAGCCTGGC ATTTTTTGAT GATTGTGTAG ATAAAGTGGA TATGGACAAG 
AGCGGTGAAG TACGACTTAT AGAACTGGAT GAATCTTTCA AAAGTGAACA CACTGTTTTT 
GTAACACCAC CTGAGATCCC CCACCTACCC AATGGTGAAG AACCCCCTTT ACAGTACAGC 
TATAATGGAT TTCCAGTTCT TAGGAACAAT TTATTTGAAA GACCTGAAGG ATTTCTACAA 
GCAAAGAAGA ACAAATTGCC TTCAAAATCA AGTAGTCCTA ATAGCCCTTT GCCCATGTTC 
AGAAGAACAA AACAGGAAAT TAAATCAGCC CATAAAATTG CAAAGAGGTA CTCTTCCATC 
CCTCAGATGT GGTCTAGGTG TCTACTGCGC CACTGTTATG GACTGTGGTT TATTTGTCTC 
CCAGCTTATG TGAAAGTCTG TCATTCAAAA GTCAGGGCTC TGAAAACAGC ATATGATGTG 
CTTAAAAAAA TGCAGTCAAA GAAGATGGAT CCACCTGATG AGGTATGCTA CCGCATTCTT 
ATGCAACTCT GTGGACAATA TGATCAGCCA GTGCTTGCAG TTCGAGTGCT TTTTGAAATG 
CAGAAAGCTG GTATTGACCC CAATGCCATT ACTTATGGTT ATTATAATAA GGCTGTTTTG 
GAAAGTACCT GGCCTTCAAG AAGTCGTAGT GGCTATTTTC TTTGGACAAA AGTAAGAAAT 
GTTGTTTTAG GAGTAACACA GTTCAAAAGA GCTTTAAAGA AGCATGCACA CTTATCACAA 
ACAACTCTCT CAGCAGATGG CAGTGACCTG GATGCTGTTA GTCATGGCAG CATGGATAGT 
GGTCATGGGA CACACACTGT GGAGCAGGCA CCTTTTAATA CGGGTTTAAT CAAAGTATAT 
GCTACTGATG ATAGATCTAG TACAGGTGGC CAGTCTGATC TTGGATATAA TTCATTATCT 
AAAGATGAAG TTAGAAGAGG GGATACATCT ACTGAAGACA TTCAAGAAGA AAAAGATAAA 
AAAGGGAGTG ATTGTAGTTC CTTGTCAGAG AGTGAGAGTA CAAAAGGAAG TGCTGATTGC 
CTTCCTAAGC TCAGTTATCA AAATTCTTCC AGTATCGTTC GCCTCACTGG TACAAGTAAC 
AACAGTGCTG GTAAAATATC AGGAGAAAGC ATGGAAAGCA CACCTGAGCT GCTCTTAATA 
TCATCTCTTG AAGATACAAA TGAAACACGA AACATTCAAA GTAGATGCTT CAGGAAAAGA 
CATAAAAGTG ACAATGAAAC TAATTTGCAG CAGCAAGTGG TCTGGGGAAA TAGAAACCGT 
AATCTTAGTG GAGGGGTACT GATGGGATTT ATGCTCAATA GAATTAACCA GGAAGCAACC 
CCTGGAGATA TAGTTGAAAA ATTGGGAGCT GATGCAAAAA TTCTTTCAAA TGTTATCTCA 
AAAAGCACGA GACCAAATAC TCTTGATATT GGGAAGCCAC CTTTAAGATC AAAAAGAGAC 
AGTCTAGAAA AGGAATCTAG TGATGATGAT ACACCTTTTG ATGGTTCTAA CTATTTGGCA 
GATAAAGTGG ATTCTCCTGT TATTTTTGAC TTAGAAGACT TAGACAGTGA AACAGATGTA 
TCAAAAGCAG GATGTGTTGC TACACAAAAT CCCAAGAGGA TTCAACGTAT GAACAGCAGC 
TTTTCAGTAA AACCTTTTGA AAAAACTGAT GTCGCAACAG GATTTGATCC CCTCTCTCTT 
TTGGTTGCTG AGACTGAACA GCAGCAAAAA GAAGAAGAGG AGGAGGATGA AGATGATAGC 
AAAAGCATTT CAACACCATC TGCTAGGCGT GATTTAGCTG AAGAAATTGT GATGTATATG 
AATAACATGA GCAGTCCTTT GACAAGTCGT ACACCAAGCA TTGATTTACA ACGGGCATGT 
GATGATAAAT TAAATAAGAA AAGTCCACCA TTGGTCAAGG CATGCAGAAG ATCTAGTCTG 
CCTCCTAATT CTCCTAAGCC AGTGAGACTT ACCAAATCTA AAAGTTACAC AAAAAGTGAG 
GAGAAACCAA GGGATAGACT TTGGTCTTCT CCAGCCTTCT CACCTACTTG CCCATTTAGG 
GAAGAATCTC AGGATACATT AACCCACTCA TCACCTTCAT TTAACTTAGA TACACTACTA 
GTACCTAAAC TAGATGTTCT AAGAAACAGT ATGTTCACTG CTGGAAAAGG AGTTGCAGAG 
AAAGCAAGCA AATGGTATTC AAGATTCACC ATGTACACCA CATCATCTAA GGATCAAAGT 
TCTGATCGTA CCAGTCTTTC TTCAGTGGGA GCCCAGGATT CTGAATCTAC CTCTTTAACA 
GATGAAGATG TCTGCCATGA GTTGGAAGGA CCTATCTCCT CTCAAGAGAC CAGTGCTACT 
TCAGGGACTA AGAGAATTGA TCTCAGCCGA ATAAGCCTGG AAAGTTCTGC ATCCTTGGAA 
GGATCTCTGT CGAAGTTTGC CTTACCTGGG AAATCAGAAG TGACATCTTC CTTCAACGCG 
AGTAATACAA ATATCTTCCA GAACTATGCA ATGGAGGTTC TCATCTCAAG TTGCTCTCGG 
TGTAGAACTT GTGATTGTCT TGTCCATGAT GAGGAAATCA TGGCTGGCTG GACAGCAGAT 
GATTCAAATC TCAATACTAC ATGCCCATTC TGTGGCAATA TCTTCTTACC CTTTCTGAAT 
ATAGAAATAA GAGATTTAAG ACGACCTGGA AGATACTTTC TAAAGTCAAG CCCATCAACA 
GAAAATATGC ACTTTCCATC CTCCATTTCA AGTCAGACGA GGCAGTCTTG CATTTCAACA 
TCAGCCTCTG GTCTTGACAC ATCTGCTCTC TCTGTTCAAG GGAATTTTGA TCTAAATAGC 
AAATCTAAAC TGCAGGAAAA TTTTTGCACC CGAAGTATTC AGATCCCTGC TAATAGATCA 
AAAACAGCTA TGTCTAAATG TCCAATATTT CCAATGGCCA GGAGTATTAG TACTTCTGGC 
CCCTTGGATA AGGAAGATAC TGGAAGACAG AAGCTCATTT CTACAGGCAG CCTGCCAGCT 
ACTTTACAAG GAGCTACAGA TTCTTTAGGT TTAGAATGGC ACCTTCCAAG TCCCGATCCT 
GTCACTGTTC CGTATCTTAG TCCTTTAGTG GTATGGAAAG AACTTGAAAG CTTATTGGAA 
AATGAAGGTG ATCATGCAAT AACAGTAGCA GACTTTGTGG ACCATCATCC AATTGTTTTT 
TGGAACCTGG TATGGTATTT CAGACGTCTT GACTTGCCCA GTAACTTACC TGGATTGATT 
CTTTCTTCTG AGCACTGTAA CAAGTATTCA AAGATTCCCC GCCACTGTAT GTCTGAAGAT 
AGCAAATATG TTTTAATACA AATGTTATGG GACAATATGA AGTTACATCA GGATCCGGGA 
CAGCCCTTGT ACATTCTCTG GAATGCACAC ACCCAAAAAT ATCCAATGGT CCATTTATTG 
CAAAAAAGTG ATAACTCATT TAACCAGGAA CTGTTGAAAA GTATGGTAAA AAGCATTAAA 
ATGAATGATG TCTATGGACC AATGAGTCAG ATTTTAGAGA CACTGAATAA GTGTCCACAT 
TTTAAAAGAC AGAGGAGTTT ATACAGAGAA ATACTATTTC TCTCACTTGT GGCACTGGGA 
AGAGAAAACA TTGATATAGA TGCTTTTGAT AAAGAGTACA AGATGGCATA CGATCGTCTC 
ACACCTAGTC AAGTCAAGAG TACACACAAC TGTGATAGAC CACCAAGTAC AGGGGTGATG 
GAATGTCGAA AAACCTTTGG AGAACCTTAT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519459.1
CDS	269..4444
Translation	MNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_011521157.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521157.2

ORF Insert Sequence:

ATGAACACAC TGAATAATTT GCATCAGCAA TTGGCAAAAT TGCAGCAGAG ACCGAGAGAT 
GATGGACTCA TGGATTTAGC AATAAATGAC TATGATTTTA ATTCTGGAAA GAGGTTGCAC 
ATGATAGATT TGGAAATCCA AGAGGCATTT TTGTTTTTCA TGGCCTCTAT TTTAAAAGGT 
TACAGATCAT ACTTAAGGCC AATAACACAA GCCCCATCTG AGACAGCCAC AGATGCAGCC 
TCTCTCTTTG CCCTACAAGC TTTCTTAAGA AGCCGGGACC GGTCACATCA AAAATTCTAT 
AACATGATGA CCAAAACACA AATGTTTATT CGCTTCATTG AAGAATGTTC TTTTGTTAGT 
GACAAAGATG CAAGCCTGGC ATTTTTTGAT GATTGTGTAG ATAAAGTGGA TATGGACAAG 
AGCGGTGAAG TACGACTTAT AGAACTGGAT GAATCTTTCA AAAGTGAACA CACTGTTTTT 
GTAACACCAC CTGAGATCCC CCACCTACCC AATGGTGAAG AACCCCCTTT ACAGTACAGC 
TATAATGGAT TTCCAGTTCT TAGGAACAAT TTATTTGAAA GACCTGAAGG ATTTCTACAA 
GCAAAGAAGA ACAAATTGCC TTCAAAATCA AGTAGTCCTA ATAGCCCTTT GCCCATGTTC 
AGAAGAACAA AACAGGAAAT TAAATCAGCC CATAAAATTG CAAAGAGGTA CTCTTCCATC 
CCTCAGATGT GGTCTAGGTG TCTACTGCGC CACTGTTATG GACTGTGGTT TATTTGTCTC 
CCAGCTTATG TGAAAGTCTG TCATTCAAAA GTCAGGGCTC TGAAAACAGC ATATGATGTG 
CTTAAAAAAA TGCAGTCAAA GAAGATGGAT CCACCTGATG AGGTATGCTA CCGCATTCTT 
ATGCAACTCT GTGGACAATA TGATCAGCCA GTGCTTGCAG TTCGAGTGCT TTTTGAAATG 
CAGAAAGCTG GTATTGACCC CAATGCCATT ACTTATGGTT ATTATAATAA GGCTGTTTTG 
GAAAGTACCT GGCCTTCAAG AAGTCGTAGT GGCTATTTTC TTTGGACAAA AGTAAGAAAT 
GTTGTTTTAG GAGTAACACA GTTCAAAAGA GCTTTAAAGA AGCATGCACA CTTATCACAA 
ACAACTCTCT CAGCAGATGG CAGTGACCTG GATGCTGTTA GTCATGGCAG CATGGATAGT 
GGTCATGGGA CACACACTGT GGAGCAGGCA CCTTTTAATA CGGGTTTAAT CAAAGTATAT 
GCTACTGATG ATAGATCTAG TACAGGTGGC CAGTCTGATC TTGGATATAA TTCATTATCT 
AAAGATGAAG TTAGAAGAGG GGATACATCT ACTGAAGACA TTCAAGAAGA AAAAGATAAA 
AAAGGGAGTG ATTGTAGTTC CTTGTCAGAG AGTGAGAGTA CAAAAGGAAG TGCTGATTGC 
CTTCCTAAGC TCAGTTATCA AAATTCTTCC AGTATCGTTC GCCTCACTGG TACAAGTAAC 
AACAGTGCTG GTAAAATATC AGGAGAAAGC ATGGAAAGCA CACCTGAGCT GCTCTTAATA 
TCATCTCTTG AAGATACAAA TGAAACACGA AACATTCAAA GTAGATGCTT CAGGAAAAGA 
CATAAAAGTG ACAATGAAAC TAATTTGCAG CAGCAAGTGG TCTGGGGAAA TAGAAACCGT 
AATCTTAGTG GAGGGGTACT GATGGGATTT ATGCTCAATA GAATTAACCA GGAAGCAACC 
CCTGGAGATA TAGTTGAAAA ATTGGGAGCT GATGCAAAAA TTCTTTCAAA TGTTATCTCA 
AAAAGCACGA GACCAAATAC TCTTGATATT GGGAAGCCAC CTTTAAGATC AAAAAGAGAC 
AGTCTAGAAA AGGAATCTAG TGATGATGAT ACACCTTTTG ATGGTTCTAA CTATTTGGCA 
GATAAAGTGG ATTCTCCTGT TATTTTTGAC TTAGAAGACT TAGACAGTGA AACAGATGTA 
TCAAAAGCAG GATGTGTTGC TACACAAAAT CCCAAGAGGA TTCAACGTAT GAACAGCAGC 
TTTTCAGTAA AACCTTTTGA AAAAACTGAT GTCGCAACAG GATTTGATCC CCTCTCTCTT 
TTGGTTGCTG AGACTGAACA GCAGCAAAAA GAAGAAGAGG AGGAGGATGA AGATGATAGC 
AAAAGCATTT CAACACCATC TGCTAGGCGT GATTTAGCTG AAGAAATTGT GATGTATATG 
AATAACATGA GCAGTCCTTT GACAAGTCGT ACACCAAGCA TTGATTTACA ACGGGCATGT 
GATGATAAAT TAAATAAGAA AAGTCCACCA TTGGTCAAGG CATGCAGAAG ATCTAGTCTG 
CCTCCTAATT CTCCTAAGCC AGTGAGACTT ACCAAATCTA AAAGTTACAC AAAAAGTGAG 
GAGAAACCAA GGGATAGACT TTGGTCTTCT CCAGCCTTCT CACCTACTTG CCCATTTAGG 
GAAGAATCTC AGGATACATT AACCCACTCA TCACCTTCAT TTAACTTAGA TACACTACTA 
GTACCTAAAC TAGATGTTCT AAGAAACAGT ATGTTCACTG CTGGAAAAGG AGTTGCAGAG 
AAAGCAAGCA AATGGTATTC AAGATTCACC ATGTACACCA CATCATCTAA GGATCAAAGT 
TCTGATCGTA CCAGTCTTTC TTCAGTGGGA GCCCAGGATT CTGAATCTAC CTCTTTAACA 
GATGAAGATG TCTGCCATGA GTTGGAAGGA CCTATCTCCT CTCAAGAGAC CAGTGCTACT 
TCAGGGACTA AGAGAATTGA TCTCAGCCGA ATAAGCCTGG AAAGTTCTGC ATCCTTGGAA 
GGATCTCTGT CGAAGTTTGC CTTACCTGGG AAATCAGAAG TGACATCTTC CTTCAACGCG 
AGTAATACAA ATATCTTCCA GAACTATGCA ATGGAGGTTC TCATCTCAAG TTGCTCTCGG 
TGTAGAACTT GTGATTGTCT TGTCCATGAT GAGGAAATCA TGGCTGGCTG GACAGCAGAT 
GATTCAAATC TCAATACTAC ATGCCCATTC TGTGGCAATA TCTTCTTACC CTTTCTGAAT 
ATAGAAATAA GAGATTTAAG ACGACCTGGA AGATACTTTC TAAAGTCAAG CCCATCAACA 
GAAAATATGC ACTTTCCATC CTCCATTTCA AGTCAGACGA GGCAGTCTTG CATTTCAACA 
TCAGCCTCTG GTCTTGACAC ATCTGCTCTC TCTGTTCAAG GGAATTTTGA TCTAAATAGC 
AAATCTAAAC TGCAGGAAAA TTTTTGCACC CGAAGTATTC AGATCCCTGC TAATAGATCA 
AAAACAGCTA TGTCTAAATG TCCAATATTT CCAATGGCCA GGAGTATTAG TACTTCTGGC 
CCCTTGGATA AGGAAGATAC TGGAAGACAG AAGCTCATTT CTACAGGCAG CCTGCCAGCT 
ACTTTACAAG GAGCTACAGA TTCTTTAGGT TTAGAATGGC ACCTTCCAAG TCCCGATCCT 
GTCACTGTTC CGTATCTTAG TCCTTTAGTG GTATGGAAAG AACTTGAAAG CTTATTGGAA 
AATGAAGGTG ATCATGCAAT AACAGTAGCA GACTTTGTGG ACCATCATCC AATTGTTTTT 
TGGAACCTGG TATGGTATTT CAGACGTCTT GACTTGCCCA GTAACTTACC TGGATTGATT 
CTTTCTTCTG AGCACTGTAA CAAGTATTCA AAGATTCCCC GCCACTGTAT GTCTGAAGAT 
AGCAAATATG TTTTAATACA AATGTTATGG GACAATATGA AGTTACATCA GGATCCGGGA 
CAGCCCTTGT ACATTCTCTG GAATGCACAC ACCCAAAAAT ATCCAATGGT CCATTTATTG 
CAAAAAAGTG ATAACTCATT TAACCAGGAA CTGTTGAAAA GTATGGTAAA AAGCATTAAA 
ATGAATGATG TCTATGGACC AATGAGTCAG ATTTTAGAGA CACTGAATAA GTGTCCACAT 
TTTAAAAGAC AGAGGAGTTT ATACAGAGAA ATACTATTTC TCTCACTTGT GGCACTGGGA 
AGAGAAAACA TTGATATAGA TGCTTTTGAT AAAGAGTACA AGATGGCATA CGATCGTCTC 
ACACCTAGTC AAGTCAAGAG TACACACAAC TGTGATAGAC CACCAAGTAC AGGGGTGATG 
GAATGTCGAA AAACCTTTGG AGAACCTTAT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DENND4A ( XM_011521157.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519459.1 Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X9, mRNA.

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CloneID	OHu63823
Clone ID Related Accession (Same CDS sequence)	XM_011521157.2 , XM_011521157.3
Accession Version	XM_011521157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-myc promoter-binding protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521157.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACACAC TGAATAATTT GCATCAGCAA TTGGCAAAAT TGCAGCAGAG ACCGAGAGAT 
GATGGACTCA TGGATTTAGC AATAAATGAC TATGATTTTA ATTCTGGAAA GAGGTTGCAC 
ATGATAGATT TGGAAATCCA AGAGGCATTT TTGTTTTTCA TGGCCTCTAT TTTAAAAGGT 
TACAGATCAT ACTTAAGGCC AATAACACAA GCCCCATCTG AGACAGCCAC AGATGCAGCC 
TCTCTCTTTG CCCTACAAGC TTTCTTAAGA AGCCGGGACC GGTCACATCA AAAATTCTAT 
AACATGATGA CCAAAACACA AATGTTTATT CGCTTCATTG AAGAATGTTC TTTTGTTAGT 
GACAAAGATG CAAGCCTGGC ATTTTTTGAT GATTGTGTAG ATAAAGTGGA TATGGACAAG 
AGCGGTGAAG TACGACTTAT AGAACTGGAT GAATCTTTCA AAAGTGAACA CACTGTTTTT 
GTAACACCAC CTGAGATCCC CCACCTACCC AATGGTGAAG AACCCCCTTT ACAGTACAGC 
TATAATGGAT TTCCAGTTCT TAGGAACAAT TTATTTGAAA GACCTGAAGG ATTTCTACAA 
GCAAAGAAGA ACAAATTGCC TTCAAAATCA AGTAGTCCTA ATAGCCCTTT GCCCATGTTC 
AGAAGAACAA AACAGGAAAT TAAATCAGCC CATAAAATTG CAAAGAGGTA CTCTTCCATC 
CCTCAGATGT GGTCTAGGTG TCTACTGCGC CACTGTTATG GACTGTGGTT TATTTGTCTC 
CCAGCTTATG TGAAAGTCTG TCATTCAAAA GTCAGGGCTC TGAAAACAGC ATATGATGTG 
CTTAAAAAAA TGCAGTCAAA GAAGATGGAT CCACCTGATG AGGTATGCTA CCGCATTCTT 
ATGCAACTCT GTGGACAATA TGATCAGCCA GTGCTTGCAG TTCGAGTGCT TTTTGAAATG 
CAGAAAGCTG GTATTGACCC CAATGCCATT ACTTATGGTT ATTATAATAA GGCTGTTTTG 
GAAAGTACCT GGCCTTCAAG AAGTCGTAGT GGCTATTTTC TTTGGACAAA AGTAAGAAAT 
GTTGTTTTAG GAGTAACACA GTTCAAAAGA GCTTTAAAGA AGCATGCACA CTTATCACAA 
ACAACTCTCT CAGCAGATGG CAGTGACCTG GATGCTGTTA GTCATGGCAG CATGGATAGT 
GGTCATGGGA CACACACTGT GGAGCAGGCA CCTTTTAATA CGGGTTTAAT CAAAGTATAT 
GCTACTGATG ATAGATCTAG TACAGGTGGC CAGTCTGATC TTGGATATAA TTCATTATCT 
AAAGATGAAG TTAGAAGAGG GGATACATCT ACTGAAGACA TTCAAGAAGA AAAAGATAAA 
AAAGGGAGTG ATTGTAGTTC CTTGTCAGAG AGTGAGAGTA CAAAAGGAAG TGCTGATTGC 
CTTCCTAAGC TCAGTTATCA AAATTCTTCC AGTATCGTTC GCCTCACTGG TACAAGTAAC 
AACAGTGCTG GTAAAATATC AGGAGAAAGC ATGGAAAGCA CACCTGAGCT GCTCTTAATA 
TCATCTCTTG AAGATACAAA TGAAACACGA AACATTCAAA GTAGATGCTT CAGGAAAAGA 
CATAAAAGTG ACAATGAAAC TAATTTGCAG CAGCAAGTGG TCTGGGGAAA TAGAAACCGT 
AATCTTAGTG GAGGGGTACT GATGGGATTT ATGCTCAATA GAATTAACCA GGAAGCAACC 
CCTGGAGATA TAGTTGAAAA ATTGGGAGCT GATGCAAAAA TTCTTTCAAA TGTTATCTCA 
AAAAGCACGA GACCAAATAC TCTTGATATT GGGAAGCCAC CTTTAAGATC AAAAAGAGAC 
AGTCTAGAAA AGGAATCTAG TGATGATGAT ACACCTTTTG ATGGTTCTAA CTATTTGGCA 
GATAAAGTGG ATTCTCCTGT TATTTTTGAC TTAGAAGACT TAGACAGTGA AACAGATGTA 
TCAAAAGCAG GATGTGTTGC TACACAAAAT CCCAAGAGGA TTCAACGTAT GAACAGCAGC 
TTTTCAGTAA AACCTTTTGA AAAAACTGAT GTCGCAACAG GATTTGATCC CCTCTCTCTT 
TTGGTTGCTG AGACTGAACA GCAGCAAAAA GAAGAAGAGG AGGAGGATGA AGATGATAGC 
AAAAGCATTT CAACACCATC TGCTAGGCGT GATTTAGCTG AAGAAATTGT GATGTATATG 
AATAACATGA GCAGTCCTTT GACAAGTCGT ACACCAAGCA TTGATTTACA ACGGGCATGT 
GATGATAAAT TAAATAAGAA AAGTCCACCA TTGGTCAAGG CATGCAGAAG ATCTAGTCTG 
CCTCCTAATT CTCCTAAGCC AGTGAGACTT ACCAAATCTA AAAGTTACAC AAAAAGTGAG 
GAGAAACCAA GGGATAGACT TTGGTCTTCT CCAGCCTTCT CACCTACTTG CCCATTTAGG 
GAAGAATCTC AGGATACATT AACCCACTCA TCACCTTCAT TTAACTTAGA TACACTACTA 
GTACCTAAAC TAGATGTTCT AAGAAACAGT ATGTTCACTG CTGGAAAAGG AGTTGCAGAG 
AAAGCAAGCA AATGGTATTC AAGATTCACC ATGTACACCA CATCATCTAA GGATCAAAGT 
TCTGATCGTA CCAGTCTTTC TTCAGTGGGA GCCCAGGATT CTGAATCTAC CTCTTTAACA 
GATGAAGATG TCTGCCATGA GTTGGAAGGA CCTATCTCCT CTCAAGAGAC CAGTGCTACT 
TCAGGGACTA AGAGAATTGA TCTCAGCCGA ATAAGCCTGG AAAGTTCTGC ATCCTTGGAA 
GGATCTCTGT CGAAGTTTGC CTTACCTGGG AAATCAGAAG TGACATCTTC CTTCAACGCG 
AGTAATACAA ATATCTTCCA GAACTATGCA ATGGAGGTTC TCATCTCAAG TTGCTCTCGG 
TGTAGAACTT GTGATTGTCT TGTCCATGAT GAGGAAATCA TGGCTGGCTG GACAGCAGAT 
GATTCAAATC TCAATACTAC ATGCCCATTC TGTGGCAATA TCTTCTTACC CTTTCTGAAT 
ATAGAAATAA GAGATTTAAG ACGACCTGGA AGATACTTTC TAAAGTCAAG CCCATCAACA 
GAAAATATGC ACTTTCCATC CTCCATTTCA AGTCAGACGA GGCAGTCTTG CATTTCAACA 
TCAGCCTCTG GTCTTGACAC ATCTGCTCTC TCTGTTCAAG GGAATTTTGA TCTAAATAGC 
AAATCTAAAC TGCAGGAAAA TTTTTGCACC CGAAGTATTC AGATCCCTGC TAATAGATCA 
AAAACAGCTA TGTCTAAATG TCCAATATTT CCAATGGCCA GGAGTATTAG TACTTCTGGC 
CCCTTGGATA AGGAAGATAC TGGAAGACAG AAGCTCATTT CTACAGGCAG CCTGCCAGCT 
ACTTTACAAG GAGCTACAGA TTCTTTAGGT TTAGAATGGC ACCTTCCAAG TCCCGATCCT 
GTCACTGTTC CGTATCTTAG TCCTTTAGTG GTATGGAAAG AACTTGAAAG CTTATTGGAA 
AATGAAGGTG ATCATGCAAT AACAGTAGCA GACTTTGTGG ACCATCATCC AATTGTTTTT 
TGGAACCTGG TATGGTATTT CAGACGTCTT GACTTGCCCA GTAACTTACC TGGATTGATT 
CTTTCTTCTG AGCACTGTAA CAAGTATTCA AAGATTCCCC GCCACTGTAT GTCTGAAGAT 
AGCAAATATG TTTTAATACA AATGTTATGG GACAATATGA AGTTACATCA GGATCCGGGA 
CAGCCCTTGT ACATTCTCTG GAATGCACAC ACCCAAAAAT ATCCAATGGT CCATTTATTG 
CAAAAAAGTG ATAACTCATT TAACCAGGAA CTGTTGAAAA GTATGGTAAA AAGCATTAAA 
ATGAATGATG TCTATGGACC AATGAGTCAG ATTTTAGAGA CACTGAATAA GTGTCCACAT 
TTTAAAAGAC AGAGGAGTTT ATACAGAGAA ATACTATTTC TCTCACTTGT GGCACTGGGA 
AGAGAAAACA TTGATATAGA TGCTTTTGAT AAAGAGTACA AGATGGCATA CGATCGTCTC 
ACACCTAGTC AAGTCAAGAG TACACACAAC TGTGATAGAC CACCAAGTAC AGGGGTGATG 
GAATGTCGAA AAACCTTTGG AGAACCTTAT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519459.1
CDS	269..4444
Translation	MNTLNNLHQQLAKLQQRPRDDGLMDLAINDYDFNSGKRLHMIDLEIQEAFLFFMASILKGYRSYLRPITQAPSETATDAASLFALQAFLRSRDRSHQKFYNMMTKTQMFIRFIEECSFVSDKDASLAFFDDCVDKVDMDKSGEVRLIELDESFKSEHTVFVTPPEIPHLPNGEEPPLQYSYNGFPVLRNNLFERPEGFLQAKKNKLPSKSSSPNSPLPMFRRTKQEIKSAHKIAKRYSSIPQMWSRCLLRHCYGLWFICLPAYVKVCHSKVRALKTAYDVLKKMQSKKMDPPDEVCYRILMQLCGQYDQPVLAVRVLFEMQKAGIDPNAITYGYYNKAVLESTWPSRSRSGYFLWTKVRNVVLGVTQFKRALKKHAHLSQTTLSADGSDLDAVSHGSMDSGHGTHTVEQAPFNTGLIKVYATDDRSSTGGQSDLGYNSLSKDEVRRGDTSTEDIQEEKDKKGSDCSSLSESESTKGSADCLPKLSYQNSSSIVRLTGTSNNSAGKISGESMESTPELLLISSLEDTNETRNIQSRCFRKRHKSDNETNLQQQVVWGNRNRNLSGGVLMGFMLNRINQEATPGDIVEKLGADAKILSNVISKSTRPNTLDIGKPPLRSKRDSLEKESSDDDTPFDGSNYLADKVDSPVIFDLEDLDSETDVSKAGCVATQNPKRIQRMNSSFSVKPFEKTDVATGFDPLSLLVAETEQQQKEEEEEDEDDSKSISTPSARRDLAEEIVMYMNNMSSPLTSRTPSIDLQRACDDKLNKKSPPLVKACRRSSLPPNSPKPVRLTKSKSYTKSEEKPRDRLWSSPAFSPTCPFREESQDTLTHSSPSFNLDTLLVPKLDVLRNSMFTAGKGVAEKASKWYSRFTMYTTSSKDQSSDRTSLSSVGAQDSESTSLTDEDVCHELEGPISSQETSATSGTKRIDLSRISLESSASLEGSLSKFALPGKSEVTSSFNASNTNIFQNYAMEVLISSCSRCRTCDCLVHDEEIMAGWTADDSNLNTTCPFCGNIFLPFLNIEIRDLRRPGRYFLKSSPSTENMHFPSSISSQTRQSCISTSASGLDTSALSVQGNFDLNSKSKLQENFCTRSIQIPANRSKTAMSKCPIFPMARSISTSGPLDKEDTGRQKLISTGSLPATLQGATDSLGLEWHLPSPDPVTVPYLSPLVVWKELESLLENEGDHAITVADFVDHHPIVFWNLVWYFRRLDLPSNLPGLILSSEHCNKYSKIPRHCMSEDSKYVLIQMLWDNMKLHQDPGQPLYILWNAHTQKYPMVHLLQKSDNSFNQELLKSMVKSIKMNDVYGPMSQILETLNKCPHFKRQRSLYREILFLSLVALGRENIDIDAFDKEYKMAYDRLTPSQVKSTHNCDRPPSTGVMECRKTFGEPYL

Target ORF information:

RefSeq Version	XM_011521157.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DENN domain containing 4A (DENND4A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521157.3

ORF Insert Sequence:

ATGAACACAC TGAATAATTT GCATCAGCAA TTGGCAAAAT TGCAGCAGAG ACCGAGAGAT 
GATGGACTCA TGGATTTAGC AATAAATGAC TATGATTTTA ATTCTGGAAA GAGGTTGCAC 
ATGATAGATT TGGAAATCCA AGAGGCATTT TTGTTTTTCA TGGCCTCTAT TTTAAAAGGT 
TACAGATCAT ACTTAAGGCC AATAACACAA GCCCCATCTG AGACAGCCAC AGATGCAGCC 
TCTCTCTTTG CCCTACAAGC TTTCTTAAGA AGCCGGGACC GGTCACATCA AAAATTCTAT 
AACATGATGA CCAAAACACA AATGTTTATT CGCTTCATTG AAGAATGTTC TTTTGTTAGT 
GACAAAGATG CAAGCCTGGC ATTTTTTGAT GATTGTGTAG ATAAAGTGGA TATGGACAAG 
AGCGGTGAAG TACGACTTAT AGAACTGGAT GAATCTTTCA AAAGTGAACA CACTGTTTTT 
GTAACACCAC CTGAGATCCC CCACCTACCC AATGGTGAAG AACCCCCTTT ACAGTACAGC 
TATAATGGAT TTCCAGTTCT TAGGAACAAT TTATTTGAAA GACCTGAAGG ATTTCTACAA 
GCAAAGAAGA ACAAATTGCC TTCAAAATCA AGTAGTCCTA ATAGCCCTTT GCCCATGTTC 
AGAAGAACAA AACAGGAAAT TAAATCAGCC CATAAAATTG CAAAGAGGTA CTCTTCCATC 
CCTCAGATGT GGTCTAGGTG TCTACTGCGC CACTGTTATG GACTGTGGTT TATTTGTCTC 
CCAGCTTATG TGAAAGTCTG TCATTCAAAA GTCAGGGCTC TGAAAACAGC ATATGATGTG 
CTTAAAAAAA TGCAGTCAAA GAAGATGGAT CCACCTGATG AGGTATGCTA CCGCATTCTT 
ATGCAACTCT GTGGACAATA TGATCAGCCA GTGCTTGCAG TTCGAGTGCT TTTTGAAATG 
CAGAAAGCTG GTATTGACCC CAATGCCATT ACTTATGGTT ATTATAATAA GGCTGTTTTG 
GAAAGTACCT GGCCTTCAAG AAGTCGTAGT GGCTATTTTC TTTGGACAAA AGTAAGAAAT 
GTTGTTTTAG GAGTAACACA GTTCAAAAGA GCTTTAAAGA AGCATGCACA CTTATCACAA 
ACAACTCTCT CAGCAGATGG CAGTGACCTG GATGCTGTTA GTCATGGCAG CATGGATAGT 
GGTCATGGGA CACACACTGT GGAGCAGGCA CCTTTTAATA CGGGTTTAAT CAAAGTATAT 
GCTACTGATG ATAGATCTAG TACAGGTGGC CAGTCTGATC TTGGATATAA TTCATTATCT 
AAAGATGAAG TTAGAAGAGG GGATACATCT ACTGAAGACA TTCAAGAAGA AAAAGATAAA 
AAAGGGAGTG ATTGTAGTTC CTTGTCAGAG AGTGAGAGTA CAAAAGGAAG TGCTGATTGC 
CTTCCTAAGC TCAGTTATCA AAATTCTTCC AGTATCGTTC GCCTCACTGG TACAAGTAAC 
AACAGTGCTG GTAAAATATC AGGAGAAAGC ATGGAAAGCA CACCTGAGCT GCTCTTAATA 
TCATCTCTTG AAGATACAAA TGAAACACGA AACATTCAAA GTAGATGCTT CAGGAAAAGA 
CATAAAAGTG ACAATGAAAC TAATTTGCAG CAGCAAGTGG TCTGGGGAAA TAGAAACCGT 
AATCTTAGTG GAGGGGTACT GATGGGATTT ATGCTCAATA GAATTAACCA GGAAGCAACC 
CCTGGAGATA TAGTTGAAAA ATTGGGAGCT GATGCAAAAA TTCTTTCAAA TGTTATCTCA 
AAAAGCACGA GACCAAATAC TCTTGATATT GGGAAGCCAC CTTTAAGATC AAAAAGAGAC 
AGTCTAGAAA AGGAATCTAG TGATGATGAT ACACCTTTTG ATGGTTCTAA CTATTTGGCA 
GATAAAGTGG ATTCTCCTGT TATTTTTGAC TTAGAAGACT TAGACAGTGA AACAGATGTA 
TCAAAAGCAG GATGTGTTGC TACACAAAAT CCCAAGAGGA TTCAACGTAT GAACAGCAGC 
TTTTCAGTAA AACCTTTTGA AAAAACTGAT GTCGCAACAG GATTTGATCC CCTCTCTCTT 
TTGGTTGCTG AGACTGAACA GCAGCAAAAA GAAGAAGAGG AGGAGGATGA AGATGATAGC 
AAAAGCATTT CAACACCATC TGCTAGGCGT GATTTAGCTG AAGAAATTGT GATGTATATG 
AATAACATGA GCAGTCCTTT GACAAGTCGT ACACCAAGCA TTGATTTACA ACGGGCATGT 
GATGATAAAT TAAATAAGAA AAGTCCACCA TTGGTCAAGG CATGCAGAAG ATCTAGTCTG 
CCTCCTAATT CTCCTAAGCC AGTGAGACTT ACCAAATCTA AAAGTTACAC AAAAAGTGAG 
GAGAAACCAA GGGATAGACT TTGGTCTTCT CCAGCCTTCT CACCTACTTG CCCATTTAGG 
GAAGAATCTC AGGATACATT AACCCACTCA TCACCTTCAT TTAACTTAGA TACACTACTA 
GTACCTAAAC TAGATGTTCT AAGAAACAGT ATGTTCACTG CTGGAAAAGG AGTTGCAGAG 
AAAGCAAGCA AATGGTATTC AAGATTCACC ATGTACACCA CATCATCTAA GGATCAAAGT 
TCTGATCGTA CCAGTCTTTC TTCAGTGGGA GCCCAGGATT CTGAATCTAC CTCTTTAACA 
GATGAAGATG TCTGCCATGA GTTGGAAGGA CCTATCTCCT CTCAAGAGAC CAGTGCTACT 
TCAGGGACTA AGAGAATTGA TCTCAGCCGA ATAAGCCTGG AAAGTTCTGC ATCCTTGGAA 
GGATCTCTGT CGAAGTTTGC CTTACCTGGG AAATCAGAAG TGACATCTTC CTTCAACGCG 
AGTAATACAA ATATCTTCCA GAACTATGCA ATGGAGGTTC TCATCTCAAG TTGCTCTCGG 
TGTAGAACTT GTGATTGTCT TGTCCATGAT GAGGAAATCA TGGCTGGCTG GACAGCAGAT 
GATTCAAATC TCAATACTAC ATGCCCATTC TGTGGCAATA TCTTCTTACC CTTTCTGAAT 
ATAGAAATAA GAGATTTAAG ACGACCTGGA AGATACTTTC TAAAGTCAAG CCCATCAACA 
GAAAATATGC ACTTTCCATC CTCCATTTCA AGTCAGACGA GGCAGTCTTG CATTTCAACA 
TCAGCCTCTG GTCTTGACAC ATCTGCTCTC TCTGTTCAAG GGAATTTTGA TCTAAATAGC 
AAATCTAAAC TGCAGGAAAA TTTTTGCACC CGAAGTATTC AGATCCCTGC TAATAGATCA 
AAAACAGCTA TGTCTAAATG TCCAATATTT CCAATGGCCA GGAGTATTAG TACTTCTGGC 
CCCTTGGATA AGGAAGATAC TGGAAGACAG AAGCTCATTT CTACAGGCAG CCTGCCAGCT 
ACTTTACAAG GAGCTACAGA TTCTTTAGGT TTAGAATGGC ACCTTCCAAG TCCCGATCCT 
GTCACTGTTC CGTATCTTAG TCCTTTAGTG GTATGGAAAG AACTTGAAAG CTTATTGGAA 
AATGAAGGTG ATCATGCAAT AACAGTAGCA GACTTTGTGG ACCATCATCC AATTGTTTTT 
TGGAACCTGG TATGGTATTT CAGACGTCTT GACTTGCCCA GTAACTTACC TGGATTGATT 
CTTTCTTCTG AGCACTGTAA CAAGTATTCA AAGATTCCCC GCCACTGTAT GTCTGAAGAT 
AGCAAATATG TTTTAATACA AATGTTATGG GACAATATGA AGTTACATCA GGATCCGGGA 
CAGCCCTTGT ACATTCTCTG GAATGCACAC ACCCAAAAAT ATCCAATGGT CCATTTATTG 
CAAAAAAGTG ATAACTCATT TAACCAGGAA CTGTTGAAAA GTATGGTAAA AAGCATTAAA 
ATGAATGATG TCTATGGACC AATGAGTCAG ATTTTAGAGA CACTGAATAA GTGTCCACAT 
TTTAAAAGAC AGAGGAGTTT ATACAGAGAA ATACTATTTC TCTCACTTGT GGCACTGGGA 
AGAGAAAACA TTGATATAGA TGCTTTTGAT AAAGAGTACA AGATGGCATA CGATCGTCTC 
ACACCTAGTC AAGTCAAGAG TACACACAAC TGTGATAGAC CACCAAGTAC AGGGGTGATG 
GAATGTCGAA AAACCTTTGG AGAACCTTAT CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DENND4A
Entrez Gene ID	10260
Full Name	DENN domain containing 4A
Synonyms	IRLB,MYCPBP
General protein information	Preferred Names DENN domain containing 4A Names C-myc promoter-binding protein DENN domain-containing protein 4A DENN/MADD domain containing 4A c-myc promoter binding protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q22.31
Summary	This gene encodes a DENN domain-containing protein that may function as a guanine nucleotide exchange factor that specifically activates ras-related protein Rab-10. This protein also contains a interferon stimulated response element-binding domain and may be involved in regulating the v-myc avian myelocytomatosis viral (MYC) oncogene. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8. [provided by RefSeq, Mar 2016].
Disorder MIM:	600382

Homo sapiens (human)	DENND4A	NP_001138295.1
Bos taurus (cattle)	DENND4A	NP_001192454.1
Gallus gallus (chicken)	DENND4A	XP_413911.4
Drosophila melanogaster (fruit fly)	Crag	NP_727281.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001102	XP_322065.3
Pan troglodytes (chimpanzee)	DENND4A	XP_523102.2
	DENND4A	XP_001109950.1
Rattus norvegicus (Norway rat)	Dennd4a	XP_006243364.1
Mus musculus (house mouse)	Dennd4a	NP_001156389.1
Canis lupus familiaris (dog)	DENND4A	XP_005638584.1
Danio rerio (zebrafish)	dennd4a	NP_001074458.1
Xenopus tropicalis (tropical clawed frog)	dennd4a	XP_004912733.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DENND4A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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