best4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	best4
Entrez Gene ID	563711
Full Name	bestrophin 4
Synonyms	si:ch211-106k21.4
General protein information	Preferred Names bestrophin 4 Names bestrophin-4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_687068.5	XP_692160.4	bestrophin-4
XM_687068.6	XP_692160.4	bestrophin-4

Pathways from BioSystems

Homologies

	BEST4	XP_001098771.1
Canis lupus familiaris (dog)	BEST4	XP_539638.1
Bos taurus (cattle)	BEST4	NP_001192608.1
Xenopus tropicalis (tropical clawed frog)	best4	NP_001116914.1
Homo sapiens (human)	BEST4	NP_695006.1
Pan troglodytes (chimpanzee)	BEST4	XP_524571.2
Rattus norvegicus (Norway rat)	Best4	XP_001066317.1
Gallus gallus (chicken)	BEST4	NP_001239054.1
Danio rerio (zebrafish)	best4	XP_692160.3
Drosophila melanogaster (fruit fly)	Best4	NP_001097609.1

Gene Ontology

The following best4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the best4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44158	XM_687068.6 Latest version!	Danio rerio bestrophin 4 (best4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa44158	XM_687068.5	Danio rerio bestrophin 4 (best4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

best4 ( XM_687068.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_692160.4 Danio rerio bestrophin 4 (best4), mRNA.

Price & Availability↑

CloneID	ODa44158
Clone ID Related Accession (Same CDS sequence)	XM_687068.5 , XM_687068.6
Accession Version	XM_687068.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	bestrophin-4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_687068.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCCA GAGTAATCAT TCATAATGCT GAGACCTGTG TGAAACTGAC ACTGGCCATG 
AATATTTCTA CTGAGTTCCC AAACATAAAT CACAAGAAGG ATCGACAGGT CCACATTACA 
GTCAGAGATA TAAAAGGCAA ATCAAGAGGT GTGCTTTTCC CTCTAAAAGG AGTCAGACTT 
GGAAATCTTA AGGATTCCAC CATGACGGTC TCCTACACGC TCAAGGTTGC CAATGCTAAG 
TTTGGAGGTT TCTCGAAGCT GCTCTTCAGA TGGAAAGGAA GCATTTACAA GCTTCTCTAC 
AGAGAATTTC TGGTTTTCTG CTTACTGTAC ACCTTTATCA GTATTCTGTA CAGGTGTGTC 
CTTAATAACA ATCAACAGGA GATATTCGCG AGACTAGCAC GTCATTGTAA CAAGTTTGCC 
AAACTCATCC CCATGTCATT TGTTCTTGGG TTCTACGTCA CTCAAGCATT TCAGCGCTGG 
TGGGGTCAGT ACACCAGCTT TCCCCTGCCT GATAACCTGA TGATGGTGGT CTCTGGAAAC 
GTCCATGGTA CAGATGAAAG AGGTCGTCTG TTGAGGAGAA CTCTGATGAG ATATGCTAAC 
CTGTCATCTG TCCTCATACT GCGTTCCATC AGCACACGAG TGCGCAAGAG GTTTACAACT 
CTGGAGGACA TTGTGGAGGC AGGATTCATG ACCACAGATG AGCTCAGGAA ACTAAACCAT 
CTGTACTCAG ACTTCAACAA ATACTGGATG CCGCTTGCAT GGTTTGCCAA CCTGGCGTCT 
CAGGCCAGAA AAGAAGGGCG TGTGAAAGAT GATATAGCAT TGCGTCTGCT AATGGATGAG 
CTGAATAACT ATAGATCAAA ATGCAGCATG CTCTTCCACT ATGACTGGAT CAGCATTCCC 
TTGGTGTACA CACAGGTGGT GACTGTGGCC GTGTACTCCT TCTTTACATT TAGTCTTATT 
GGGAGGCAGT TTGTGAAGCC TGCATCTTCT GAAGAACACC TTGACCTGTA CATCCCCATT 
TTCACTTTAC TGGAGTTCTT CTTTTATGCT GGATGGTTAA AGGTAGGGGA ACTTATCATC 
AACCCGTTTG GAGAAGATGA TGATGATTTT GAAACCAACC AACTCATAGA TCGAAACATT 
CAGGTTTCTA TGCTAGCCGT AGATGACATG CACCAGAATC TTCCTCCTCT TGAGAAGGAC 
AAATACTGGG TGGATAAAGG TCCTTCATAT CCTTCGGCTT CTATGAAGCC TGTTTTCATG 
GGTTCTACAC ATGACATGAG GATACTTGAG GATGATCTTG AAGAATGCGT GTCAACCCCC 
AAGACTCAGA TGCTTGCCAT TAATGTGTGG CCGACTACAC ACAAAGTGAA AAAGTCGAAA 
AGGAGTTTGC AACAATTATG TATTTGCTGC AGAGCTGAAG GTGAGGATGA CTGCTCTAAA 
CTTGAACCTT CCAATAGCAG CGTCCATTCA CCACAACACA AGACTGTTCA ACACAGCTGC 
CAAGAAAAAC TCTTTGGGTC ACAGGAAACT ACAGGACCAG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_692160.4
CDS	1671..3218
Translation	MFSRVIIHNAETCVKLTLAMNISTEFPNINHKKDRQVHITVRDIKGKSRGVLFPLKGVRLGNLKDSTMTVSYTLKVANAKFGGFSKLLFRWKGSIYKLLYREFLVFCLLYTFISILYRCVLNNNQQEIFARLARHCNKFAKLIPMSFVLGFYVTQAFQRWWGQYTSFPLPDNLMMVVSGNVHGTDERGRLLRRTLMRYANLSSVLILRSISTRVRKRFTTLEDIVEAGFMTTDELRKLNHLYSDFNKYWMPLAWFANLASQARKEGRVKDDIALRLLMDELNNYRSKCSMLFHYDWISIPLVYTQVVTVAVYSFFTFSLIGRQFVKPASSEEHLDLYIPIFTLLEFFFYAGWLKVGELIINPFGEDDDDFETNQLIDRNIQVSMLAVDDMHQNLPPLEKDKYWVDKGPSYPSASMKPVFMGSTHDMRILEDDLEECVSTPKTQMLAINVWPTTHKVKKSKRSLQQLCICCRAEGEDDCSKLEPSNSSVHSPQHKTVQHSCQEKLFGSQETTGPAN

Target ORF information:

RefSeq Version	XM_687068.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio bestrophin 4 (best4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_687068.5

ORF Insert Sequence:

ATGTTTTCCA GAGTAATCAT TCATAATGCT GAGACCTGTG TGAAACTGAC ACTGGCCATG 
AATATTTCTA CTGAGTTCCC AAACATAAAT CACAAGAAGG ATCGACAGGT CCACATTACA 
GTCAGAGATA TAAAAGGCAA ATCAAGAGGT GTGCTTTTCC CTCTAAAAGG AGTCAGACTT 
GGAAATCTTA AGGATTCCAC CATGACGGTC TCCTACACGC TCAAGGTTGC CAATGCTAAG 
TTTGGAGGTT TCTCGAAGCT GCTCTTCAGA TGGAAAGGAA GCATTTACAA GCTTCTCTAC 
AGAGAATTTC TGGTTTTCTG CTTACTGTAC ACCTTTATCA GTATTCTGTA CAGGTGTGTC 
CTTAATAACA ATCAACAGGA GATATTCGCG AGACTAGCAC GTCATTGTAA CAAGTTTGCC 
AAACTCATCC CCATGTCATT TGTTCTTGGG TTCTACGTCA CTCAAGCATT TCAGCGCTGG 
TGGGGTCAGT ACACCAGCTT TCCCCTGCCT GATAACCTGA TGATGGTGGT CTCTGGAAAC 
GTCCATGGTA CAGATGAAAG AGGTCGTCTG TTGAGGAGAA CTCTGATGAG ATATGCTAAC 
CTGTCATCTG TCCTCATACT GCGTTCCATC AGCACACGAG TGCGCAAGAG GTTTACAACT 
CTGGAGGACA TTGTGGAGGC AGGATTCATG ACCACAGATG AGCTCAGGAA ACTAAACCAT 
CTGTACTCAG ACTTCAACAA ATACTGGATG CCGCTTGCAT GGTTTGCCAA CCTGGCGTCT 
CAGGCCAGAA AAGAAGGGCG TGTGAAAGAT GATATAGCAT TGCGTCTGCT AATGGATGAG 
CTGAATAACT ATAGATCAAA ATGCAGCATG CTCTTCCACT ATGACTGGAT CAGCATTCCC 
TTGGTGTACA CACAGGTGGT GACTGTGGCC GTGTACTCCT TCTTTACATT TAGTCTTATT 
GGGAGGCAGT TTGTGAAGCC TGCATCTTCT GAAGAACACC TTGACCTGTA CATCCCCATT 
TTCACTTTAC TGGAGTTCTT CTTTTATGCT GGATGGTTAA AGGTAGGGGA ACTTATCATC 
AACCCGTTTG GAGAAGATGA TGATGATTTT GAAACCAACC AACTCATAGA TCGAAACATT 
CAGGTTTCTA TGCTAGCCGT AGATGACATG CACCAGAATC TTCCTCCTCT TGAGAAGGAC 
AAATACTGGG TGGATAAAGG TCCTTCATAT CCTTCGGCTT CTATGAAGCC TGTTTTCATG 
GGTTCTACAC ATGACATGAG GATACTTGAG GATGATCTTG AAGAATGCGT GTCAACCCCC 
AAGACTCAGA TGCTTGCCAT TAATGTGTGG CCGACTACAC ACAAAGTGAA AAAGTCGAAA 
AGGAGTTTGC AACAATTATG TATTTGCTGC AGAGCTGAAG GTGAGGATGA CTGCTCTAAA 
CTTGAACCTT CCAATAGCAG CGTCCATTCA CCACAACACA AGACTGTTCA ACACAGCTGC 
CAAGAAAAAC TCTTTGGGTC ACAGGAAACT ACAGGACCAG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

best4 ( XM_687068.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_692160.4 Danio rerio bestrophin 4 (best4), mRNA.

Price & Availability↑

CloneID	ODa44158
Clone ID Related Accession (Same CDS sequence)	XM_687068.5 , XM_687068.6
Accession Version	XM_687068.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	bestrophin-4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_687068.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCCA GAGTAATCAT TCATAATGCT GAGACCTGTG TGAAACTGAC ACTGGCCATG 
AATATTTCTA CTGAGTTCCC AAACATAAAT CACAAGAAGG ATCGACAGGT CCACATTACA 
GTCAGAGATA TAAAAGGCAA ATCAAGAGGT GTGCTTTTCC CTCTAAAAGG AGTCAGACTT 
GGAAATCTTA AGGATTCCAC CATGACGGTC TCCTACACGC TCAAGGTTGC CAATGCTAAG 
TTTGGAGGTT TCTCGAAGCT GCTCTTCAGA TGGAAAGGAA GCATTTACAA GCTTCTCTAC 
AGAGAATTTC TGGTTTTCTG CTTACTGTAC ACCTTTATCA GTATTCTGTA CAGGTGTGTC 
CTTAATAACA ATCAACAGGA GATATTCGCG AGACTAGCAC GTCATTGTAA CAAGTTTGCC 
AAACTCATCC CCATGTCATT TGTTCTTGGG TTCTACGTCA CTCAAGCATT TCAGCGCTGG 
TGGGGTCAGT ACACCAGCTT TCCCCTGCCT GATAACCTGA TGATGGTGGT CTCTGGAAAC 
GTCCATGGTA CAGATGAAAG AGGTCGTCTG TTGAGGAGAA CTCTGATGAG ATATGCTAAC 
CTGTCATCTG TCCTCATACT GCGTTCCATC AGCACACGAG TGCGCAAGAG GTTTACAACT 
CTGGAGGACA TTGTGGAGGC AGGATTCATG ACCACAGATG AGCTCAGGAA ACTAAACCAT 
CTGTACTCAG ACTTCAACAA ATACTGGATG CCGCTTGCAT GGTTTGCCAA CCTGGCGTCT 
CAGGCCAGAA AAGAAGGGCG TGTGAAAGAT GATATAGCAT TGCGTCTGCT AATGGATGAG 
CTGAATAACT ATAGATCAAA ATGCAGCATG CTCTTCCACT ATGACTGGAT CAGCATTCCC 
TTGGTGTACA CACAGGTGGT GACTGTGGCC GTGTACTCCT TCTTTACATT TAGTCTTATT 
GGGAGGCAGT TTGTGAAGCC TGCATCTTCT GAAGAACACC TTGACCTGTA CATCCCCATT 
TTCACTTTAC TGGAGTTCTT CTTTTATGCT GGATGGTTAA AGGTAGGGGA ACTTATCATC 
AACCCGTTTG GAGAAGATGA TGATGATTTT GAAACCAACC AACTCATAGA TCGAAACATT 
CAGGTTTCTA TGCTAGCCGT AGATGACATG CACCAGAATC TTCCTCCTCT TGAGAAGGAC 
AAATACTGGG TGGATAAAGG TCCTTCATAT CCTTCGGCTT CTATGAAGCC TGTTTTCATG 
GGTTCTACAC ATGACATGAG GATACTTGAG GATGATCTTG AAGAATGCGT GTCAACCCCC 
AAGACTCAGA TGCTTGCCAT TAATGTGTGG CCGACTACAC ACAAAGTGAA AAAGTCGAAA 
AGGAGTTTGC AACAATTATG TATTTGCTGC AGAGCTGAAG GTGAGGATGA CTGCTCTAAA 
CTTGAACCTT CCAATAGCAG CGTCCATTCA CCACAACACA AGACTGTTCA ACACAGCTGC 
CAAGAAAAAC TCTTTGGGTC ACAGGAAACT ACAGGACCAG CAAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_692160.4
CDS	880..2427
Translation	MFSRVIIHNAETCVKLTLAMNISTEFPNINHKKDRQVHITVRDIKGKSRGVLFPLKGVRLGNLKDSTMTVSYTLKVANAKFGGFSKLLFRWKGSIYKLLYREFLVFCLLYTFISILYRCVLNNNQQEIFARLARHCNKFAKLIPMSFVLGFYVTQAFQRWWGQYTSFPLPDNLMMVVSGNVHGTDERGRLLRRTLMRYANLSSVLILRSISTRVRKRFTTLEDIVEAGFMTTDELRKLNHLYSDFNKYWMPLAWFANLASQARKEGRVKDDIALRLLMDELNNYRSKCSMLFHYDWISIPLVYTQVVTVAVYSFFTFSLIGRQFVKPASSEEHLDLYIPIFTLLEFFFYAGWLKVGELIINPFGEDDDDFETNQLIDRNIQVSMLAVDDMHQNLPPLEKDKYWVDKGPSYPSASMKPVFMGSTHDMRILEDDLEECVSTPKTQMLAINVWPTTHKVKKSKRSLQQLCICCRAEGEDDCSKLEPSNSSVHSPQHKTVQHSCQEKLFGSQETTGPAN

Target ORF information:

RefSeq Version	XM_687068.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio bestrophin 4 (best4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_687068.6

ORF Insert Sequence:

ATGTTTTCCA GAGTAATCAT TCATAATGCT GAGACCTGTG TGAAACTGAC ACTGGCCATG 
AATATTTCTA CTGAGTTCCC AAACATAAAT CACAAGAAGG ATCGACAGGT CCACATTACA 
GTCAGAGATA TAAAAGGCAA ATCAAGAGGT GTGCTTTTCC CTCTAAAAGG AGTCAGACTT 
GGAAATCTTA AGGATTCCAC CATGACGGTC TCCTACACGC TCAAGGTTGC CAATGCTAAG 
TTTGGAGGTT TCTCGAAGCT GCTCTTCAGA TGGAAAGGAA GCATTTACAA GCTTCTCTAC 
AGAGAATTTC TGGTTTTCTG CTTACTGTAC ACCTTTATCA GTATTCTGTA CAGGTGTGTC 
CTTAATAACA ATCAACAGGA GATATTCGCG AGACTAGCAC GTCATTGTAA CAAGTTTGCC 
AAACTCATCC CCATGTCATT TGTTCTTGGG TTCTACGTCA CTCAAGCATT TCAGCGCTGG 
TGGGGTCAGT ACACCAGCTT TCCCCTGCCT GATAACCTGA TGATGGTGGT CTCTGGAAAC 
GTCCATGGTA CAGATGAAAG AGGTCGTCTG TTGAGGAGAA CTCTGATGAG ATATGCTAAC 
CTGTCATCTG TCCTCATACT GCGTTCCATC AGCACACGAG TGCGCAAGAG GTTTACAACT 
CTGGAGGACA TTGTGGAGGC AGGATTCATG ACCACAGATG AGCTCAGGAA ACTAAACCAT 
CTGTACTCAG ACTTCAACAA ATACTGGATG CCGCTTGCAT GGTTTGCCAA CCTGGCGTCT 
CAGGCCAGAA AAGAAGGGCG TGTGAAAGAT GATATAGCAT TGCGTCTGCT AATGGATGAG 
CTGAATAACT ATAGATCAAA ATGCAGCATG CTCTTCCACT ATGACTGGAT CAGCATTCCC 
TTGGTGTACA CACAGGTGGT GACTGTGGCC GTGTACTCCT TCTTTACATT TAGTCTTATT 
GGGAGGCAGT TTGTGAAGCC TGCATCTTCT GAAGAACACC TTGACCTGTA CATCCCCATT 
TTCACTTTAC TGGAGTTCTT CTTTTATGCT GGATGGTTAA AGGTAGGGGA ACTTATCATC 
AACCCGTTTG GAGAAGATGA TGATGATTTT GAAACCAACC AACTCATAGA TCGAAACATT 
CAGGTTTCTA TGCTAGCCGT AGATGACATG CACCAGAATC TTCCTCCTCT TGAGAAGGAC 
AAATACTGGG TGGATAAAGG TCCTTCATAT CCTTCGGCTT CTATGAAGCC TGTTTTCATG 
GGTTCTACAC ATGACATGAG GATACTTGAG GATGATCTTG AAGAATGCGT GTCAACCCCC 
AAGACTCAGA TGCTTGCCAT TAATGTGTGG CCGACTACAC ACAAAGTGAA AAAGTCGAAA 
AGGAGTTTGC AACAATTATG TATTTGCTGC AGAGCTGAAG GTGAGGATGA CTGCTCTAAA 
CTTGAACCTT CCAATAGCAG CGTCCATTCA CCACAACACA AGACTGTTCA ACACAGCTGC 
CAAGAAAAAC TCTTTGGGTC ACAGGAAACT ACAGGACCAG CAAACTGA