si:ch211-285f17.1 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005162585.4	XP_005162642.1	sickle tail protein homolog isoform X2
XM_005162585.3	XP_005162642.1	sickle tail protein homolog isoform X1
XM_009297304.2	XP_009295579.1	sickle tail protein homolog isoform X3
XM_009297306.2	XP_009295581.1	sickle tail protein isoform X11
XM_005162592.3	XP_005162649.1	sickle tail protein homolog isoform X9
XM_017353919.1	XP_017209408.1	sickle tail protein homolog isoform X4
XM_005162589.3	XP_005162646.1	sickle tail protein homolog isoform X5
XM_005162590.3	XP_005162647.1	sickle tail protein homolog isoform X6
XM_021470275.1	XP_021325950.1	sickle tail protein homolog isoform X3
XM_021470277.1	XP_021325952.1	sickle tail protein homolog isoform X5
XM_021470279.1	XP_021325954.1	sickle tail protein homolog isoform X7
XM_021470280.1	XP_021325955.1	sickle tail protein homolog isoform X8
XM_685844.9	XP_690936.6	sickle tail protein homolog isoform X11
XM_009297305.2	XP_009295580.1	sickle tail protein isoform X10
XM_021470276.1	XP_021325951.1	sickle tail protein homolog isoform X4
XM_021470281.1	XP_021325956.1	sickle tail protein homolog isoform X9
XM_021470282.1	XP_021325957.1	sickle tail protein homolog isoform X12
XM_021470285.1	XP_021325960.1	sickle tail protein homolog isoform X15
XM_005162591.3	XP_005162648.1	sickle tail protein homolog isoform X7
XM_021470278.1	XP_021325953.1	sickle tail protein homolog isoform X6
XM_021470283.1	XP_021325958.1	sickle tail protein isoform X13
XM_685844.8	XP_690936.6	sickle tail protein homolog isoform X8
XM_005162587.3	XP_005162644.1	sickle tail protein homolog isoform X2
XM_021470274.1	XP_021325949.1	sickle tail protein homolog isoform X1
XM_005162591.4	XP_005162648.1	sickle tail protein homolog isoform X10
XM_021470284.1	XP_021325959.1	sickle tail protein isoform X14

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151782	XM_021470284.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa151783	XM_021470285.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa151775	XM_021470277.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa151776	XM_021470278.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa151777	XM_021470279.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa151778	XM_021470280.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa151779	XM_021470281.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa151780	XM_021470282.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa151781	XM_021470283.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa57669	XM_017353919.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa28377	XM_005162589.3 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa28378	XM_005162590.3 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa28379	XM_005162591.4 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa28380	XM_685844.9 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa28381	XM_005162592.3 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43821	XM_009297305.2 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa43822	XM_009297306.2 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa151772	XM_021470274.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa151773	XM_021470275.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa151774	XM_021470276.1 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa28373	XM_005162585.4 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa28375	XM_005162587.3 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa43820	XM_009297304.2 Latest version!	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
ODa28373	XM_005162585.3	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
ODa28380	XM_685844.8	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa28379	XM_005162591.3	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

si:ch211-285f17.1 ( XM_021470284.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325959.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa151782
Clone ID Related Accession (Same CDS sequence)	XM_021470284.1
Accession Version	XM_021470284.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4422bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGA ACGCCAACGT GGTCTCACCT ACTAGTCGGA TGCCAGTGCC AGTTTCTGTC 
AAGGCGCGGC AGCCATCAAG CACCTCAGAA AAAGAGAGGG CAACAAAACC GCAAAAACTA 
CAGGACTCTC AGAGGCAGTT CCGCCAGGCT AATGGAAGTG CTAAGAGATC TGGAGGAGAT 
CCCAAATCCA CTTCTCCTAC CGTGCCTGCT TCTAAAATTC CCGCTTTTTC CACTAGTACG 
GGAAAAAGCT TGTCCCAGTC TGATTCTACT AATAATGTTA ATTCTTCTTC TCCTTTATCT 
TCTTCCCTTC CCACCAGCAA ATCCTCTATC CCTCCTCCTC GATCCAGTTC CATCCCCTCC 
TCCCACATCC CCTCTATTTC TAACGGTTCT TTAAAATATG CCCCTCCCAC TCACAGCACT 
AAAGGCCTCT CTATTCCCAC ACAGACACAA AATGGTCGAC CTTCCACTTC TTCTTCCTCC 
TCCTGTTCCC ACTCCCCCTT GTCCCCGACT ATGTTGAGTC AGAGTGCGAA GAGTATCCGT 
ACCATTCACA CACCCAGCTT CAACAGCTAC AGTCGGCCGC ACAACGGAAG TGCCGGTAAA 
TCAGCTATAC CAACGGTAAC TGTCGCTAAG GATGCTGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325959.1
CDS	593..5014
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470284.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470284.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGA ACGCCAACGT GGTCTCACCT ACTAGTCGGA TGCCAGTGCC AGTTTCTGTC 
AAGGCGCGGC AGCCATCAAG CACCTCAGAA AAAGAGAGGG CAACAAAACC GCAAAAACTA 
CAGGACTCTC AGAGGCAGTT CCGCCAGGCT AATGGAAGTG CTAAGAGATC TGGAGGAGAT 
CCCAAATCCA CTTCTCCTAC CGTGCCTGCT TCTAAAATTC CCGCTTTTTC CACTAGTACG 
GGAAAAAGCT TGTCCCAGTC TGATTCTACT AATAATGTTA ATTCTTCTTC TCCTTTATCT 
TCTTCCCTTC CCACCAGCAA ATCCTCTATC CCTCCTCCTC GATCCAGTTC CATCCCCTCC 
TCCCACATCC CCTCTATTTC TAACGGTTCT TTAAAATATG CCCCTCCCAC TCACAGCACT 
AAAGGCCTCT CTATTCCCAC ACAGACACAA AATGGTCGAC CTTCCACTTC TTCTTCCTCC 
TCCTGTTCCC ACTCCCCCTT GTCCCCGACT ATGTTGAGTC AGAGTGCGAA GAGTATCCGT 
ACCATTCACA CACCCAGCTT CAACAGCTAC AGTCGGCCGC ACAACGGAAG TGCCGGTAAA 
TCAGCTATAC CAACGGTAAC TGTCGCTAAG GATGCTGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470285.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325960.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ODa151783
Clone ID Related Accession (Same CDS sequence)	XM_021470285.1
Accession Version	XM_021470285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGA CACAAAATGG TCGACCTTCC ACTTCTTCTT CCTCCTCCTG TTCCCACTCC 
CCCTTGTCCC CGACTATGTT GAGTCAGAGT GCGAAGAGTA TCCGTACCAT TCACACACCC 
AGCTTCAACA GCTACAGTCG GCCGCACAAC GGAAGTGCCG GTAAATCAGC TATACCAACG 
GTAACTGTCG CTAAGGATGC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325960.1
CDS	596..4582
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470285.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470285.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGA CACAAAATGG TCGACCTTCC ACTTCTTCTT CCTCCTCCTG TTCCCACTCC 
CCCTTGTCCC CGACTATGTT GAGTCAGAGT GCGAAGAGTA TCCGTACCAT TCACACACCC 
AGCTTCAACA GCTACAGTCG GCCGCACAAC GGAAGTGCCG GTAAATCAGC TATACCAACG 
GTAACTGTCG CTAAGGATGC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470277.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325952.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa151775
Clone ID Related Accession (Same CDS sequence)	XM_021470277.1
Accession Version	XM_021470277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGAC ACCGGGTTCA GTTCGCGGAT CTTCCTTCTA CCAGCGAGCG CAAAGGCAGC 
CCCAAAATCA GCAAGCCTCT GAAGGGTAAT CTAAAGGTGA CTTCCAGTGA AGACAGCGAA 
CACCTGACCC GTAAACAGCA GGTTGTCTCT GCTAACAGCT CTGGTAACCG CTGTGAGGCC 
AAATCCAGCC GCGCAGGGGT TCCACGCAGA CACACTGTGG GTGGAGCGCG CAGCTCACGG 
GAGATCCTGG CCATGCAGCC CTCGGATATG GATAAAAAAC GAGAGGCTTT TCTGGAACAT 
CTGAAGCAGA AGTATCCGCA CCACGCCTCT GCCATAATGG GCCATCAGGA GAGACTCAGA 
GAACAGTCAT TTCAGAGGAT GCTGTCGGCT TTGCACTCTG AGATAGACAT TCAAAGATAT 
CTGCTGAAAA TCCAGGCTGC TGGTTCACGC AGAACCCGGG GCTCTAAGCA GAGTGTGGGG 
TGTCCGAGTC CAGTGCCTGT TTTTGTGGAT CAGGTAGAGC ACGGCTCACT GGTCTCTCTA 
GATGCTCTGG AGGTCATGTC TGAGTGTGAT ATGCCCCTCG CCTTCACCCG AGGCTCCCGC 
TCTCGAGCCA GTCTGCCAGT GGTCCGCTCT ACTAACCAGA CCAAGGATCG CTCACTAGGA 
GTGCTGTATC TGCAGTATGG GGACGACACA AAGCAGATCC GCATGCCTAA TGAGATAACA 
AGTGTTGACA CAATCAGAGC CCTGTTCGTT AGTGCCTTCC CTCATCAGCT CAACATGAAG 
ATGCTGGAGT CGCCCAGCAC TGCCATCTAC GTCAAAGATG AGACCAGAAA TGTGTATTAT 
GAACTCACCG ATGTCAGGAG CATCACAGAT CACTCCTGCC TGAAAGTGTA TCATAAAGAC 
CCAGCACACG CCTTCAGTCA CGGACCCCGG CCGAGCAATG GAGACACAAG GGTTCACAGG 
GAAATGATAT TTGCTGGACG GGATGGTCAC CCACTCCGGA ACCCCCCAAT GGGTCCTCCA 
CCCCCCCACA TGCAAGGCTC CATGTCACCC ACTACACCTC ACGGGATGCC TCCCTCTCCA 
TCTCGAATTC CCTTTGGCCC GCGAAACCCA GGTGGAGGTG TTGCCACAAT GCCACGTGAG 
CGAGCCGGAC ACACACTCCC AGCCCCTGCC CGAGCATCCT CCCCCTGCCC GAGTGCCATC 
TTGGAGCGGC GGGATGTCAA ACCTGACGAG GATGTAGCTG CGAAGACCAT GTCTCTGAAC 
ACCAGAGCTG AAAGTCATTA CGCCGACCCT TATATGTTAC TTCACCATGC CCCGCCCCCT 
GACACAGTAG ACCACGCCTA CCACCGTGCT ACCATGCGCT CTTACAGCAA CCCCAGCGTT 
CCCTTGGAGC CCCCAGACCA TCTCTTCCGA CAGAAAAGCA GGAATAGCCA ACTCCCCATG 
CTTGGCTCCA AAACCCCGCC CCCTTCCCCG CACCGAATGG GTGAGATGAG AATGGTGGAC 
ATCCATACGG TCCAACCGCA GAGCTCCATG ACTCTGGAAA GGGCTTCACC TGTTAGACAA 
TCATTTCGGA AGGAGGCTCC TCCTATGGAT GCCATGGTAA AGGCAAGAGG GGGAATGGGG 
TCTCCAGCCA CCCCCGATCT TCAGGTTCAC AATCCTCTGG CGCCCCCGAC AGACCCTCAA 
ACACGAGAGA GAATGAAGGC CATGGAGGAG CAAATAGCCA GTCTGACTGG TCTTGTGCAG 
CACGCTCTCT TAAAGAGTAA CAGCACTAAC AGCAACAAGG AGCACTCCAG CGAAAGACCA 
GTGAAGTCTG GATCACCAGC TCACAGCACA TCTAGCACAG GAGGCTCTCC AGTATTAGCA 
CCAAAGGTTG TTGCTGCTCC TCCTGAGCGT AGCCCCGCCC CCACACCACC TTTAGGACCT 
GCCCCTTTGC ATGTTAACCT TCATCTGTTC AGGAAGAATG TCTCTGAGCT CCGCCTCCAA 
CTACAACATA TGAGACAACT ACAGCTTCAG AATCAGGAGT GTGTACGTGC TCAGATAAAG 
CGCGCAGAAC AGGAGATCGG TGTGAAACTG GCTGAAATGC TGAGACGACA GGATGATCCG 
ACACAGAAAC AGAGGATGCT TGTGGAGGAA GAGAGACACC GATATCTCAG CATGCAGGAA 
AGAGTTCTCA CACAGCTGGG GGATCTGGAG CAGTTTGTGG AGACTCTGAG GACTGATTCG 
GGTCCTGCTG CGTCTCAGAG AGCAGTGACG CTGAAGGAGG TTGAGGATGG GGCTGTCAGC 
CTGAGGAAGG TGGGAGAAAA CCTGGCTGGA CTCAAAGGTG AGTTTCCGGC TCTTCAGTCC 
CGCATGCGCT CAGTGCTCAG GGTTGAAGTA GAGGCTGTAA AGTTCCTCAA AGAAGAGCCA 
CACAAACTGG ACAGCATGTT AAAGAGAGTC CGGACCCTCA CTGACACACT CAGCAACCTT 
CGCAGGTGTG CCACCGATAA CCTGCTTAAA CCCACTGATC AGGTTTGCTC AACACCTCCT 
GCCTCTGAAA AACCAGAACT TATTGAATCC CCAGAACCTG CACCGGTCAC ACCTCAGAGC 
TCCATCGTGA GGTCAGAGGT TATGACCTCA AACCCTGTGG TGATCCACCA TGCTCAGGCC 
TCTCCAGTAG CATCACAACA ATCCCAGCAA TCAACAGCAC CTTCACCCAT CCCTACACAA 
AGAAGAGAGT CTACACCGTC ACCCACTAAA CAGCTCAGAA AACCCTCACT GGAACAAACA 
CAAAACAACA CCAGTAATGG AGTTTCTGCT AATGGCGTAC ATGGCAAGGG TCCTTCTCCA 
AACCTGATCA TTGAGGAGAT CCACGCCAGC CACAGCAAGA ACAAGAACAG AGCTGTGTCC 
ATAGAGGCTG CAGAGAAGGA ATGGGAGGAG AAAAGGCAGA ACTTGGGTCA TTATGATGGA 
CAGGAGTTTG AGCGGATTCT CCAGGAAGCA GAAGCAAACA TGCTGAGGGG AATACCAAAC 
CTCGAGGTGC CCAGTCAACT TGAGGGTTCT CCTGCTCCAC CTGCCATGCT TCCTGCCTCT 
CTGGAGGAGG TAGTAGAAAT AAACGAACCA GTAGCAGCAC CAAGAGAACA CGATGACAAT 
CAGCCAAACT CTGAGAAACC AGCTAAGACT GCTTCAGAGA AACCTCCCAA ACCTCTGGAG 
AAACCAGTTA AGCCAGCCTT GGAGAAACCT CTTAAACCCA GCCTATCCAT CAAATCCAAC 
CTAGAGAGAC AGAGTAAGAC TCAGGAAAAA GTTAAACTTC AGAGCACAGA GAAAACCAAC 
AAGTCTCCTC CTCCACCCCC ACCTCGACGC AACTACACCA CCAACACCGG CATGACCACC 
ACCCGCTCTG GAGAGGTCAT TTACACCAGC AGGAAAGAGT CTGTAACGGA AGGTGAAGAG 
GAGTCCAACA TTTCAACAAT GTCTCAGCCT GAACCAAAGA CCCCCCCAGA GGTAAAGCCC 
AAACCCATCA CCCCTCCACC TATCTCTGCC TCAGCCATCA CTGAAGAAGA GGACGAAGGA 
GACAAGATTA TGGCAGAGCT ACAGGTTTTC CAGAAGTGCT CTGTTAAGGA GGTAGGGCTC 
AAGGGTTTGG TAGAGCCACA GATCAGAGAG CTAAGACCTG GGGTCTTACT GCCCCTTAAA 
GATAAGAAGA ACACAGAGAA CCAAGATGAC AAGAATACTG ACACAGATGA GAATGGCAAC 
AATACTATTC GTCAAAGTCC TGGGGTCATC TACTATGTCA CAGCTCAGAT CTCCAAGGGA 
ACTCCACCAC CGTCAGAGGA ATCAACTGAG CGTAGAGATG CATCGCAGTC TTTCCCATCA 
CAGGTGTCAC AAATCAATGC TTCTGACCAA TCCCAAAGCC AGCAGCAGGT TTCCAATAAC 
AAATCAGATG CCCTCAAAAA CATTGGCCTA GCCAACTTTT CTGGCACCCA GCAACAAAAT 
CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA CATCCAGCCT CTTCATGGGA AGAAGTTCCT 
GGTAGCCCAA CACAAGGGAA GGTTCATGTG GTCAAGGCTG CTCAAGTGCA GGTTAGCATA 
GAGGAGAGGG TAGAGTCTCC AACAGCTGTT TGCTCACCAG TCTCTTTTGG AGACTCTGAA 
CCCATCCTTT CTAAAGTGAT CCCTGTCGAG ACAGCTTTGA CAAATCAAAA GCTTCCATTT 
GGTTCATCCA AGGTGAAGGA AAGGAAATTT GAGCAAATGG AGGAAAATAA TGAGGCTTTT 
CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA CCTCCTCCAC CACCTGATAA CTTTGCCTTT 
ATGATAACCA ACACCAAGGT TCAAGCCCTC TCCACTGGAG AGTACCATCA GCTTGTCAAC 
GCCAAGAAAG GAAGTGTACA GACAGTGACT TTTGGCAACA AACAACCAAG TGCTTCTTAC 
AGTGGCATGG CATCAGGAGG TGATGCTAAT ACCAATGGCC CTCCTGGAGC TGATAACACT 
AGTAAAAAGC CAGTTATCAT AATCTTTGAT GAGCCAATGG ACATCCGTTC AGCTTACAAG 
CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG GAGCTAGATC GGATGCTTGC AGAAGAAAGA 
ATAGATGAGG AGAGTGAGGA AACTGAGGAA GAAGAATGGA CGAGAGGAGA GAAGGTCAAA 
CAGAATGGAG TTGAAAGACA GTCTGTAAAA AGAACTCTTG CAGATGTCAC AGATGGTGAA 
GTCAAGAACT CTTCATCATC CTTACTGGTT GCTGGTGATG CAAAGCAAGA TGGGAAGAAA 
AAGTTTAAGT TGAAATTTCC CAAGAAACAG CTAGCAGCAT TAACACAAGC TATTCGGGCT 
GGAAGTAAAA CAGGCAAAAA GACTTTGCAA GTTGTCGTCT ATGAGGATGA GGAAGAGTCC 
GATGGCACTG TCAAGCAACA TAAAGAGACC AAGAGGTATG AAATTGCTAG CTCCAAAACA 
GAATCCTCAA AATCAAATAC ACAGGAATCT AATGGACGAA CTGATGAGAT TCGTAAGAGC 
ACCTACAAAA CACTTGACAG TCTAGAGCAA ACAATAAAGC AGCTAGAGAA TACAATTTGC 
GAAATGGGAC CAACGTCTTC GGAAGACACT GAAAGCCTAA CGAATTCTGA AACTCAAGAA 
AACAAAGTAG TGGAGAATTC AGGCCACTCA GAGAAGGGAC TTTCTTCCAA AACCTTGGTT 
CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT AGTCTCCGAA AGAAGACCAA ACCACAGCTC 
CTTCCTCGGC CTACCAACAT ACCAACTACA GGGGTCTCAG TAACACCAGT CGCTACCCAC 
CAGAACGCCA ACGTGGTCTC ACCTACTAGT CGGATGCCAG TGCCAGTTTC TGTCAAGGCG 
CGGCAGCCAT CAAGCACCTC AGAAAAAGAG AGGGCAACAA AACCGCAAAA ACTACAGGAC 
TCTCAGAGGC AGTTCCGCCA GGCTAATGGA AGTGCTAAGA GATCTGGAGG AGATCCCAAA 
TCCACTTCTC CTACCGTGCC TGCTTCTAAA ATTCCCGCTT TTTCCACTAG TACGGGAAAA 
AGCTTGTCCC AGTCTGATTC TACTAATAAT GTTAATTCTT CTTCTCCTTT ATCTTCTTCC 
CTTCCCACCA GCAAATCCTC TATCCCTCCT CCTCGATCCA GTTCCATCCC CTCCTCCCAC 
ATCCCCTCTA TTTCTAACGG TTCTTTAAAA TATGCCCCTC CCACTCACAG CACTAAAGGC 
CTCTCTATTC CCACACAGAC ACAAAATGGT CGACCTTCCA CTTCTTCTTC CTCCTCCTGT 
TCCCACTCCC CCTTGTCCCC GACTATGTTG AGTCAGAGTG CGAAGAGTAT CCGTACCATT 
CACACACCCA GCTTCAACAG CTACAGTCGG CCGCACAACG GAAGTGCCGG TAAATCAGCT 
ATACCAACGG TAACTGTCGC TAAGGATGCT GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325952.1
CDS	122..6097
Translation	MSGHRVQFADLPSTSERKGSPKISKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470277.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470277.1

ORF Insert Sequence:

ATGTCCGGAC ACCGGGTTCA GTTCGCGGAT CTTCCTTCTA CCAGCGAGCG CAAAGGCAGC 
CCCAAAATCA GCAAGCCTCT GAAGGGTAAT CTAAAGGTGA CTTCCAGTGA AGACAGCGAA 
CACCTGACCC GTAAACAGCA GGTTGTCTCT GCTAACAGCT CTGGTAACCG CTGTGAGGCC 
AAATCCAGCC GCGCAGGGGT TCCACGCAGA CACACTGTGG GTGGAGCGCG CAGCTCACGG 
GAGATCCTGG CCATGCAGCC CTCGGATATG GATAAAAAAC GAGAGGCTTT TCTGGAACAT 
CTGAAGCAGA AGTATCCGCA CCACGCCTCT GCCATAATGG GCCATCAGGA GAGACTCAGA 
GAACAGTCAT TTCAGAGGAT GCTGTCGGCT TTGCACTCTG AGATAGACAT TCAAAGATAT 
CTGCTGAAAA TCCAGGCTGC TGGTTCACGC AGAACCCGGG GCTCTAAGCA GAGTGTGGGG 
TGTCCGAGTC CAGTGCCTGT TTTTGTGGAT CAGGTAGAGC ACGGCTCACT GGTCTCTCTA 
GATGCTCTGG AGGTCATGTC TGAGTGTGAT ATGCCCCTCG CCTTCACCCG AGGCTCCCGC 
TCTCGAGCCA GTCTGCCAGT GGTCCGCTCT ACTAACCAGA CCAAGGATCG CTCACTAGGA 
GTGCTGTATC TGCAGTATGG GGACGACACA AAGCAGATCC GCATGCCTAA TGAGATAACA 
AGTGTTGACA CAATCAGAGC CCTGTTCGTT AGTGCCTTCC CTCATCAGCT CAACATGAAG 
ATGCTGGAGT CGCCCAGCAC TGCCATCTAC GTCAAAGATG AGACCAGAAA TGTGTATTAT 
GAACTCACCG ATGTCAGGAG CATCACAGAT CACTCCTGCC TGAAAGTGTA TCATAAAGAC 
CCAGCACACG CCTTCAGTCA CGGACCCCGG CCGAGCAATG GAGACACAAG GGTTCACAGG 
GAAATGATAT TTGCTGGACG GGATGGTCAC CCACTCCGGA ACCCCCCAAT GGGTCCTCCA 
CCCCCCCACA TGCAAGGCTC CATGTCACCC ACTACACCTC ACGGGATGCC TCCCTCTCCA 
TCTCGAATTC CCTTTGGCCC GCGAAACCCA GGTGGAGGTG TTGCCACAAT GCCACGTGAG 
CGAGCCGGAC ACACACTCCC AGCCCCTGCC CGAGCATCCT CCCCCTGCCC GAGTGCCATC 
TTGGAGCGGC GGGATGTCAA ACCTGACGAG GATGTAGCTG CGAAGACCAT GTCTCTGAAC 
ACCAGAGCTG AAAGTCATTA CGCCGACCCT TATATGTTAC TTCACCATGC CCCGCCCCCT 
GACACAGTAG ACCACGCCTA CCACCGTGCT ACCATGCGCT CTTACAGCAA CCCCAGCGTT 
CCCTTGGAGC CCCCAGACCA TCTCTTCCGA CAGAAAAGCA GGAATAGCCA ACTCCCCATG 
CTTGGCTCCA AAACCCCGCC CCCTTCCCCG CACCGAATGG GTGAGATGAG AATGGTGGAC 
ATCCATACGG TCCAACCGCA GAGCTCCATG ACTCTGGAAA GGGCTTCACC TGTTAGACAA 
TCATTTCGGA AGGAGGCTCC TCCTATGGAT GCCATGGTAA AGGCAAGAGG GGGAATGGGG 
TCTCCAGCCA CCCCCGATCT TCAGGTTCAC AATCCTCTGG CGCCCCCGAC AGACCCTCAA 
ACACGAGAGA GAATGAAGGC CATGGAGGAG CAAATAGCCA GTCTGACTGG TCTTGTGCAG 
CACGCTCTCT TAAAGAGTAA CAGCACTAAC AGCAACAAGG AGCACTCCAG CGAAAGACCA 
GTGAAGTCTG GATCACCAGC TCACAGCACA TCTAGCACAG GAGGCTCTCC AGTATTAGCA 
CCAAAGGTTG TTGCTGCTCC TCCTGAGCGT AGCCCCGCCC CCACACCACC TTTAGGACCT 
GCCCCTTTGC ATGTTAACCT TCATCTGTTC AGGAAGAATG TCTCTGAGCT CCGCCTCCAA 
CTACAACATA TGAGACAACT ACAGCTTCAG AATCAGGAGT GTGTACGTGC TCAGATAAAG 
CGCGCAGAAC AGGAGATCGG TGTGAAACTG GCTGAAATGC TGAGACGACA GGATGATCCG 
ACACAGAAAC AGAGGATGCT TGTGGAGGAA GAGAGACACC GATATCTCAG CATGCAGGAA 
AGAGTTCTCA CACAGCTGGG GGATCTGGAG CAGTTTGTGG AGACTCTGAG GACTGATTCG 
GGTCCTGCTG CGTCTCAGAG AGCAGTGACG CTGAAGGAGG TTGAGGATGG GGCTGTCAGC 
CTGAGGAAGG TGGGAGAAAA CCTGGCTGGA CTCAAAGGTG AGTTTCCGGC TCTTCAGTCC 
CGCATGCGCT CAGTGCTCAG GGTTGAAGTA GAGGCTGTAA AGTTCCTCAA AGAAGAGCCA 
CACAAACTGG ACAGCATGTT AAAGAGAGTC CGGACCCTCA CTGACACACT CAGCAACCTT 
CGCAGGTGTG CCACCGATAA CCTGCTTAAA CCCACTGATC AGGTTTGCTC AACACCTCCT 
GCCTCTGAAA AACCAGAACT TATTGAATCC CCAGAACCTG CACCGGTCAC ACCTCAGAGC 
TCCATCGTGA GGTCAGAGGT TATGACCTCA AACCCTGTGG TGATCCACCA TGCTCAGGCC 
TCTCCAGTAG CATCACAACA ATCCCAGCAA TCAACAGCAC CTTCACCCAT CCCTACACAA 
AGAAGAGAGT CTACACCGTC ACCCACTAAA CAGCTCAGAA AACCCTCACT GGAACAAACA 
CAAAACAACA CCAGTAATGG AGTTTCTGCT AATGGCGTAC ATGGCAAGGG TCCTTCTCCA 
AACCTGATCA TTGAGGAGAT CCACGCCAGC CACAGCAAGA ACAAGAACAG AGCTGTGTCC 
ATAGAGGCTG CAGAGAAGGA ATGGGAGGAG AAAAGGCAGA ACTTGGGTCA TTATGATGGA 
CAGGAGTTTG AGCGGATTCT CCAGGAAGCA GAAGCAAACA TGCTGAGGGG AATACCAAAC 
CTCGAGGTGC CCAGTCAACT TGAGGGTTCT CCTGCTCCAC CTGCCATGCT TCCTGCCTCT 
CTGGAGGAGG TAGTAGAAAT AAACGAACCA GTAGCAGCAC CAAGAGAACA CGATGACAAT 
CAGCCAAACT CTGAGAAACC AGCTAAGACT GCTTCAGAGA AACCTCCCAA ACCTCTGGAG 
AAACCAGTTA AGCCAGCCTT GGAGAAACCT CTTAAACCCA GCCTATCCAT CAAATCCAAC 
CTAGAGAGAC AGAGTAAGAC TCAGGAAAAA GTTAAACTTC AGAGCACAGA GAAAACCAAC 
AAGTCTCCTC CTCCACCCCC ACCTCGACGC AACTACACCA CCAACACCGG CATGACCACC 
ACCCGCTCTG GAGAGGTCAT TTACACCAGC AGGAAAGAGT CTGTAACGGA AGGTGAAGAG 
GAGTCCAACA TTTCAACAAT GTCTCAGCCT GAACCAAAGA CCCCCCCAGA GGTAAAGCCC 
AAACCCATCA CCCCTCCACC TATCTCTGCC TCAGCCATCA CTGAAGAAGA GGACGAAGGA 
GACAAGATTA TGGCAGAGCT ACAGGTTTTC CAGAAGTGCT CTGTTAAGGA GGTAGGGCTC 
AAGGGTTTGG TAGAGCCACA GATCAGAGAG CTAAGACCTG GGGTCTTACT GCCCCTTAAA 
GATAAGAAGA ACACAGAGAA CCAAGATGAC AAGAATACTG ACACAGATGA GAATGGCAAC 
AATACTATTC GTCAAAGTCC TGGGGTCATC TACTATGTCA CAGCTCAGAT CTCCAAGGGA 
ACTCCACCAC CGTCAGAGGA ATCAACTGAG CGTAGAGATG CATCGCAGTC TTTCCCATCA 
CAGGTGTCAC AAATCAATGC TTCTGACCAA TCCCAAAGCC AGCAGCAGGT TTCCAATAAC 
AAATCAGATG CCCTCAAAAA CATTGGCCTA GCCAACTTTT CTGGCACCCA GCAACAAAAT 
CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA CATCCAGCCT CTTCATGGGA AGAAGTTCCT 
GGTAGCCCAA CACAAGGGAA GGTTCATGTG GTCAAGGCTG CTCAAGTGCA GGTTAGCATA 
GAGGAGAGGG TAGAGTCTCC AACAGCTGTT TGCTCACCAG TCTCTTTTGG AGACTCTGAA 
CCCATCCTTT CTAAAGTGAT CCCTGTCGAG ACAGCTTTGA CAAATCAAAA GCTTCCATTT 
GGTTCATCCA AGGTGAAGGA AAGGAAATTT GAGCAAATGG AGGAAAATAA TGAGGCTTTT 
CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA CCTCCTCCAC CACCTGATAA CTTTGCCTTT 
ATGATAACCA ACACCAAGGT TCAAGCCCTC TCCACTGGAG AGTACCATCA GCTTGTCAAC 
GCCAAGAAAG GAAGTGTACA GACAGTGACT TTTGGCAACA AACAACCAAG TGCTTCTTAC 
AGTGGCATGG CATCAGGAGG TGATGCTAAT ACCAATGGCC CTCCTGGAGC TGATAACACT 
AGTAAAAAGC CAGTTATCAT AATCTTTGAT GAGCCAATGG ACATCCGTTC AGCTTACAAG 
CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG GAGCTAGATC GGATGCTTGC AGAAGAAAGA 
ATAGATGAGG AGAGTGAGGA AACTGAGGAA GAAGAATGGA CGAGAGGAGA GAAGGTCAAA 
CAGAATGGAG TTGAAAGACA GTCTGTAAAA AGAACTCTTG CAGATGTCAC AGATGGTGAA 
GTCAAGAACT CTTCATCATC CTTACTGGTT GCTGGTGATG CAAAGCAAGA TGGGAAGAAA 
AAGTTTAAGT TGAAATTTCC CAAGAAACAG CTAGCAGCAT TAACACAAGC TATTCGGGCT 
GGAAGTAAAA CAGGCAAAAA GACTTTGCAA GTTGTCGTCT ATGAGGATGA GGAAGAGTCC 
GATGGCACTG TCAAGCAACA TAAAGAGACC AAGAGGTATG AAATTGCTAG CTCCAAAACA 
GAATCCTCAA AATCAAATAC ACAGGAATCT AATGGACGAA CTGATGAGAT TCGTAAGAGC 
ACCTACAAAA CACTTGACAG TCTAGAGCAA ACAATAAAGC AGCTAGAGAA TACAATTTGC 
GAAATGGGAC CAACGTCTTC GGAAGACACT GAAAGCCTAA CGAATTCTGA AACTCAAGAA 
AACAAAGTAG TGGAGAATTC AGGCCACTCA GAGAAGGGAC TTTCTTCCAA AACCTTGGTT 
CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT AGTCTCCGAA AGAAGACCAA ACCACAGCTC 
CTTCCTCGGC CTACCAACAT ACCAACTACA GGGGTCTCAG TAACACCAGT CGCTACCCAC 
CAGAACGCCA ACGTGGTCTC ACCTACTAGT CGGATGCCAG TGCCAGTTTC TGTCAAGGCG 
CGGCAGCCAT CAAGCACCTC AGAAAAAGAG AGGGCAACAA AACCGCAAAA ACTACAGGAC 
TCTCAGAGGC AGTTCCGCCA GGCTAATGGA AGTGCTAAGA GATCTGGAGG AGATCCCAAA 
TCCACTTCTC CTACCGTGCC TGCTTCTAAA ATTCCCGCTT TTTCCACTAG TACGGGAAAA 
AGCTTGTCCC AGTCTGATTC TACTAATAAT GTTAATTCTT CTTCTCCTTT ATCTTCTTCC 
CTTCCCACCA GCAAATCCTC TATCCCTCCT CCTCGATCCA GTTCCATCCC CTCCTCCCAC 
ATCCCCTCTA TTTCTAACGG TTCTTTAAAA TATGCCCCTC CCACTCACAG CACTAAAGGC 
CTCTCTATTC CCACACAGAC ACAAAATGGT CGACCTTCCA CTTCTTCTTC CTCCTCCTGT 
TCCCACTCCC CCTTGTCCCC GACTATGTTG AGTCAGAGTG CGAAGAGTAT CCGTACCATT 
CACACACCCA GCTTCAACAG CTACAGTCGG CCGCACAACG GAAGTGCCGG TAAATCAGCT 
ATACCAACGG TAACTGTCGC TAAGGATGCT GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470278.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325953.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X6, mRNA.

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CloneID	ODa151776
Clone ID Related Accession (Same CDS sequence)	XM_021470278.1
Accession Version	XM_021470278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC ATGGAGACAT GAACAGGACA GACTTGCCTG GGGAAGAGCC TCTGAAGGGT 
AATCTAAAGG TGACTTCCAG TGAAGACAGC GAACACCTGA CCCGTAAACA GCAGGTTGTC 
TCTGCTAACA GCTCTGGTAA CCGCTGTGAG GCCAAATCCA GCCGCGCAGG GGTTCCACGC 
AGACACACTG TGGGTGGAGC GCGCAGCTCA CGGGAGATCC TGGCCATGCA GCCCTCGGAT 
ATGGATAAAA AACGAGAGGC TTTTCTGGAA CATCTGAAGC AGAAGTATCC GCACCACGCC 
TCTGCCATAA TGGGCCATCA GGAGAGACTC AGAGAACAGT CATTTCAGAG GATGCTGTCG 
GCTTTGCACT CTGAGATAGA CATTCAAAGA TATCTGCTGA AAATCCAGGC TGCTGGTTCA 
CGCAGAACCC GGGGCTCTAA GCAGAGTGTG GGGTGTCCGA GTCCAGTGCC TGTTTTTGTG 
GATCAGGTAG AGCACGGCTC ACTGGTCTCT CTAGATGCTC TGGAGGTCAT GTCTGAGTGT 
GATATGCCCC TCGCCTTCAC CCGAGGCTCC CGCTCTCGAG CCAGTCTGCC AGTGGTCCGC 
TCTACTAACC AGACCAAGGA TCGCTCACTA GGAGTGCTGT ATCTGCAGTA TGGGGACGAC 
ACAAAGCAGA TCCGCATGCC TAATGAGATA ACAAGTGTTG ACACAATCAG AGCCCTGTTC 
GTTAGTGCCT TCCCTCATCA GCTCAACATG AAGATGCTGG AGTCGCCCAG CACTGCCATC 
TACGTCAAAG ATGAGACCAG AAATGTGTAT TATGAACTCA CCGATGTCAG GAGCATCACA 
GATCACTCCT GCCTGAAAGT GTATCATAAA GACCCAGCAC ACGCCTTCAG TCACGGACCC 
CGGCCGAGCA ATGGAGACAC AAGGGTTCAC AGGGAAATGA TATTTGCTGG ACGGGATGGT 
CACCCACTCC GGAACCCCCC AATGGGTCCT CCACCCCCCC ACATGCAAGG CTCCATGTCA 
CCCACTACAC CTCACGGGAT GCCTCCCTCT CCATCTCGAA TTCCCTTTGG CCCGCGAAAC 
CCAGGTGGAG GTGTTGCCAC AATGCCACGT GAGCGAGCCG GACACACACT CCCAGCCCCT 
GCCCGAGCAT CCTCCCCCTG CCCGAGTGCC ATCTTGGAGC GGCGGGATGT CAAACCTGAC 
GAGGATGTAG CTGCGAAGAC CATGTCTCTG AACACCAGAG CTGAAAGTCA TTACGCCGAC 
CCTTATATGT TACTTCACCA TGCCCCGCCC CCTGACACAG TAGACCACGC CTACCACCGT 
GCTACCATGC GCTCTTACAG CAACCCCAGC GTTCCCTTGG AGCCCCCAGA CCATCTCTTC 
CGACAGAAAA GCAGGAATAG CCAACTCCCC ATGCTTGGCT CCAAAACCCC GCCCCCTTCC 
CCGCACCGAA TGGGTGAGAT GAGAATGGTG GACATCCATA CGGTCCAACC GCAGAGCTCC 
ATGACTCTGG AAAGGGCTTC ACCTGTTAGA CAATCATTTC GGAAGGAGGC TCCTCCTATG 
GATGCCATGG TAAAGGCAAG AGGGGGAATG GGGTCTCCAG CCACCCCCGA TCTTCAGGTT 
CACAATCCTC TGGCGCCCCC GACAGACCCT CAAACACGAG AGAGAATGAA GGCCATGGAG 
GAGCAAATAG CCAGTCTGAC TGGTCTTGTG CAGCACGCTC TCTTAAAGAG TAACAGCACT 
AACAGCAACA AGGAGCACTC CAGCGAAAGA CCAGTGAAGT CTGGATCACC AGCTCACAGC 
ACATCTAGCA CAGGAGGCTC TCCAGTATTA GCACCAAAGG TTGTTGCTGC TCCTCCTGAG 
CGTAGCCCCG CCCCCACACC ACCTTTAGGA CCTGCCCCTT TGCATGTTAA CCTTCATCTG 
TTCAGGAAGA ATGTCTCTGA GCTCCGCCTC CAACTACAAC ATATGAGACA ACTACAGCTT 
CAGAATCAGG AGTGTGTACG TGCTCAGATA AAGCGCGCAG AACAGGAGAT CGGTGTGAAA 
CTGGCTGAAA TGCTGAGACG ACAGGATGAT CCGACACAGA AACAGAGGAT GCTTGTGGAG 
GAAGAGAGAC ACCGATATCT CAGCATGCAG GAAAGAGTTC TCACACAGCT GGGGGATCTG 
GAGCAGTTTG TGGAGACTCT GAGGACTGAT TCGGGTCCTG CTGCGTCTCA GAGAGCAGTG 
ACGCTGAAGG AGGTTGAGGA TGGGGCTGTC AGCCTGAGGA AGGTGGGAGA AAACCTGGCT 
GGACTCAAAG GTGAGTTTCC GGCTCTTCAG TCCCGCATGC GCTCAGTGCT CAGGGTTGAA 
GTAGAGGCTG TAAAGTTCCT CAAAGAAGAG CCACACAAAC TGGACAGCAT GTTAAAGAGA 
GTCCGGACCC TCACTGACAC ACTCAGCAAC CTTCGCAGGT GTGCCACCGA TAACCTGCTT 
AAACCCACTG ATCAGGTTTG CTCAACACCT CCTGCCTCTG AAAAACCAGA ACTTATTGAA 
TCCCCAGAAC CTGCACCGGT CACACCTCAG AGCTCCATCG TGAGGTCAGA GGTTATGACC 
TCAAACCCTG TGGTGATCCA CCATGCTCAG GCCTCTCCAG TAGCATCACA ACAATCCCAG 
CAATCAACAG CACCTTCACC CATCCCTACA CAAAGAAGAG AGTCTACACC GTCACCCACT 
AAACAGCTCA GAAAACCCTC ACTGGAACAA ACACAAAACA ACACCAGTAA TGGAGTTTCT 
GCTAATGGCG TACATGGCAA GGGTCCTTCT CCAAACCTGA TCATTGAGGA GATCCACGCC 
AGCCACAGCA AGAACAAGAA CAGAGCTGTG TCCATAGAGG CTGCAGAGAA GGAATGGGAG 
GAGAAAAGGC AGAACTTGGG TCATTATGAT GGACAGGAGT TTGAGCGGAT TCTCCAGGAA 
GCAGAAGCAA ACATGCTGAG GGGAATACCA AACCTCGAGG TGCCCAGTCA ACTTGAGGGT 
TCTCCTGCTC CACCTGCCAT GCTTCCTGCC TCTCTGGAGG AGGTAGTAGA AATAAACGAA 
CCAGTAGCAG CACCAAGAGA ACACGATGAC AATCAGCCAA ACTCTGAGAA ACCAGCTAAG 
ACTGCTTCAG AGAAACCTCC CAAACCTCTG GAGAAACCAG TTAAGCCAGC CTTGGAGAAA 
CCTCTTAAAC CCAGCCTATC CATCAAATCC AACCTAGAGA GACAGAGTAA GACTCAGGAA 
AAAGTTAAAC TTCAGAGCAC AGAGAAAACC AACAAGTCTC CTCCTCCACC CCCACCTCGA 
CGCAACTACA CCACCAACAC CGGCATGACC ACCACCCGCT CTGGAGAGGT CATTTACACC 
AGCAGGAAAG AGTCTGTAAC GGAAGGTGAA GAGGAGTCCA ACATTTCAAC AATGTCTCAG 
CCTGAACCAA AGACCCCCCC AGAGGTAAAG CCCAAACCCA TCACCCCTCC ACCTATCTCT 
GCCTCAGCCA TCACTGAAGA AGAGGACGAA GGAGACAAGA TTATGGCAGA GCTACAGGTT 
TTCCAGAAGT GCTCTGTTAA GGAGGTAGGG CTCAAGGGTT TGGTAGAGCC ACAGATCAGA 
GAGCTAAGAC CTGGGGTCTT ACTGCCCCTT AAAGATAAGA AGAACACAGA GAACCAAGAT 
GACAAGAATA CTGACACAGA TGAGAATGGC AACAATACTA TTCGTCAAAG TCCTGGGGTC 
ATCTACTATG TCACAGCTCA GATCTCCAAG GGAACTCCAC CACCGTCAGA GGAATCAACT 
GAGCGTAGAG ATGCATCGCA GTCTTTCCCA TCACAGGTGT CACAAATCAA TGCTTCTGAC 
CAATCCCAAA GCCAGCAGCA GGTTTCCAAT AACAAATCAG ATGCCCTCAA AAACATTGGC 
CTAGCCAACT TTTCTGGCAC CCAGCAACAA AATCAGCTGT CTCGTTCAGC CTCACTAAAG 
TTACATCCAG CCTCTTCATG GGAAGAAGTT CCTGGTAGCC CAACACAAGG GAAGGTTCAT 
GTGGTCAAGG CTGCTCAAGT GCAGGTTAGC ATAGAGGAGA GGGTAGAGTC TCCAACAGCT 
GTTTGCTCAC CAGTCTCTTT TGGAGACTCT GAACCCATCC TTTCTAAAGT GATCCCTGTC 
GAGACAGCTT TGACAAATCA AAAGCTTCCA TTTGGTTCAT CCAAGGTGAA GGAAAGGAAA 
TTTGAGCAAA TGGAGGAAAA TAATGAGGCT TTTCTCAGTC CCGATTTGTC AGGAGAAGAG 
CCACCTCCTC CACCACCTGA TAACTTTGCC TTTATGATAA CCAACACCAA GGTTCAAGCC 
CTCTCCACTG GAGAGTACCA TCAGCTTGTC AACGCCAAGA AAGGAAGTGT ACAGACAGTG 
ACTTTTGGCA ACAAACAACC AAGTGCTTCT TACAGTGGCA TGGCATCAGG AGGTGATGCT 
AATACCAATG GCCCTCCTGG AGCTGATAAC ACTAGTAAAA AGCCAGTTAT CATAATCTTT 
GATGAGCCAA TGGACATCCG TTCAGCTTAC AAGCGCCTCT CTACCGTTTT CGAATGTGAG 
GAGGAGCTAG ATCGGATGCT TGCAGAAGAA AGAATAGATG AGGAGAGTGA GGAAACTGAG 
GAAGAAGAAT GGACGAGAGG AGAGAAGGTC AAACAGAATG GAGTTGAAAG ACAGTCTGTA 
AAAAGAACTC TTGCAGATGT CACAGATGGT GAAGTCAAGA ACTCTTCATC ATCCTTACTG 
GTTGCTGGTG ATGCAAAGCA AGATGGGAAG AAAAAGTTTA AGTTGAAATT TCCCAAGAAA 
CAGCTAGCAG CATTAACACA AGCTATTCGG GCTGGAAGTA AAACAGGCAA AAAGACTTTG 
CAAGTTGTCG TCTATGAGGA TGAGGAAGAG TCCGATGGCA CTGTCAAGCA ACATAAAGAG 
ACCAAGAGGT ATGAAATTGC TAGCTCCAAA ACAGAATCCT CAAAATCAAA TACACAGGAA 
TCTAATGGAC GAACTGATGA GATTCGTAAG AGCACCTACA AAACACTTGA CAGTCTAGAG 
CAAACAATAA AGCAGCTAGA GAATACAATT TGCGAAATGG GACCAACGTC TTCGGAAGAC 
ACTGAAAGCC TAACGAATTC TGAAACTCAA GAAAACAAAG TAGTGGAGAA TTCAGGCCAC 
TCAGAGAAGG GACTTTCTTC CAAAACCTTG GTTCCTAGGT TGTCTGCTGC CAGTAAGGGT 
CCTAGTCTCC GAAAGAAGAC CAAACCACAG CTCCTTCCTC GGCCTACCAA CATACCAACT 
ACAGGGGTCT CAGTAACACC AGTCGCTACC CACCAGAACG CCAACGTGGT CTCACCTACT 
AGTCGGATGC CAGTGCCAGT TTCTGTCAAG GCGCGGCAGC CATCAAGCAC CTCAGAAAAA 
GAGAGGGCAA CAAAACCGCA AAAACTACAG GACTCTCAGA GGCAGTTCCG CCAGGCTAAT 
GGAAGTGCTA AGAGATCTGG AGGAGATCCC AAATCCACTT CTCCTACCGT GCCTGCTTCT 
AAAATTCCCG CTTTTTCCAC TAGTACGGGA AAAAGCTTGT CCCAGTCTGA TTCTACTAAT 
AATGTTAATT CTTCTTCTCC TTTATCTTCT TCCCTTCCCA CCAGCAAATC CTCTATCCCT 
CCTCCTCGAT CCAGTTCCAT CCCCTCCTCC CACATCCCCT CTATTTCTAA CGGTTCTTTA 
AAATATGCCC CTCCCACTCA CAGCACTAAA GGCCTCTCTA TTCCCACACA GACACAAAAT 
GGTCGACCTT CCACTTCTTC TTCCTCCTCC TGTTCCCACT CCCCCTTGTC CCCGACTATG 
TTGAGTCAGA GTGCGAAGAG TATCCGTACC ATTCACACAC CCAGCTTCAA CAGCTACAGT 
CGGCCGCACA ACGGAAGTGC CGGTAAATCA GCTATACCAA CGGTAACTGT CGCTAAGGAT 
GCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325953.1
CDS	227..6175
Translation	MAEHGDMNRTDLPGEEPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470278.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470278.1

ORF Insert Sequence:

ATGGCAGAAC ATGGAGACAT GAACAGGACA GACTTGCCTG GGGAAGAGCC TCTGAAGGGT 
AATCTAAAGG TGACTTCCAG TGAAGACAGC GAACACCTGA CCCGTAAACA GCAGGTTGTC 
TCTGCTAACA GCTCTGGTAA CCGCTGTGAG GCCAAATCCA GCCGCGCAGG GGTTCCACGC 
AGACACACTG TGGGTGGAGC GCGCAGCTCA CGGGAGATCC TGGCCATGCA GCCCTCGGAT 
ATGGATAAAA AACGAGAGGC TTTTCTGGAA CATCTGAAGC AGAAGTATCC GCACCACGCC 
TCTGCCATAA TGGGCCATCA GGAGAGACTC AGAGAACAGT CATTTCAGAG GATGCTGTCG 
GCTTTGCACT CTGAGATAGA CATTCAAAGA TATCTGCTGA AAATCCAGGC TGCTGGTTCA 
CGCAGAACCC GGGGCTCTAA GCAGAGTGTG GGGTGTCCGA GTCCAGTGCC TGTTTTTGTG 
GATCAGGTAG AGCACGGCTC ACTGGTCTCT CTAGATGCTC TGGAGGTCAT GTCTGAGTGT 
GATATGCCCC TCGCCTTCAC CCGAGGCTCC CGCTCTCGAG CCAGTCTGCC AGTGGTCCGC 
TCTACTAACC AGACCAAGGA TCGCTCACTA GGAGTGCTGT ATCTGCAGTA TGGGGACGAC 
ACAAAGCAGA TCCGCATGCC TAATGAGATA ACAAGTGTTG ACACAATCAG AGCCCTGTTC 
GTTAGTGCCT TCCCTCATCA GCTCAACATG AAGATGCTGG AGTCGCCCAG CACTGCCATC 
TACGTCAAAG ATGAGACCAG AAATGTGTAT TATGAACTCA CCGATGTCAG GAGCATCACA 
GATCACTCCT GCCTGAAAGT GTATCATAAA GACCCAGCAC ACGCCTTCAG TCACGGACCC 
CGGCCGAGCA ATGGAGACAC AAGGGTTCAC AGGGAAATGA TATTTGCTGG ACGGGATGGT 
CACCCACTCC GGAACCCCCC AATGGGTCCT CCACCCCCCC ACATGCAAGG CTCCATGTCA 
CCCACTACAC CTCACGGGAT GCCTCCCTCT CCATCTCGAA TTCCCTTTGG CCCGCGAAAC 
CCAGGTGGAG GTGTTGCCAC AATGCCACGT GAGCGAGCCG GACACACACT CCCAGCCCCT 
GCCCGAGCAT CCTCCCCCTG CCCGAGTGCC ATCTTGGAGC GGCGGGATGT CAAACCTGAC 
GAGGATGTAG CTGCGAAGAC CATGTCTCTG AACACCAGAG CTGAAAGTCA TTACGCCGAC 
CCTTATATGT TACTTCACCA TGCCCCGCCC CCTGACACAG TAGACCACGC CTACCACCGT 
GCTACCATGC GCTCTTACAG CAACCCCAGC GTTCCCTTGG AGCCCCCAGA CCATCTCTTC 
CGACAGAAAA GCAGGAATAG CCAACTCCCC ATGCTTGGCT CCAAAACCCC GCCCCCTTCC 
CCGCACCGAA TGGGTGAGAT GAGAATGGTG GACATCCATA CGGTCCAACC GCAGAGCTCC 
ATGACTCTGG AAAGGGCTTC ACCTGTTAGA CAATCATTTC GGAAGGAGGC TCCTCCTATG 
GATGCCATGG TAAAGGCAAG AGGGGGAATG GGGTCTCCAG CCACCCCCGA TCTTCAGGTT 
CACAATCCTC TGGCGCCCCC GACAGACCCT CAAACACGAG AGAGAATGAA GGCCATGGAG 
GAGCAAATAG CCAGTCTGAC TGGTCTTGTG CAGCACGCTC TCTTAAAGAG TAACAGCACT 
AACAGCAACA AGGAGCACTC CAGCGAAAGA CCAGTGAAGT CTGGATCACC AGCTCACAGC 
ACATCTAGCA CAGGAGGCTC TCCAGTATTA GCACCAAAGG TTGTTGCTGC TCCTCCTGAG 
CGTAGCCCCG CCCCCACACC ACCTTTAGGA CCTGCCCCTT TGCATGTTAA CCTTCATCTG 
TTCAGGAAGA ATGTCTCTGA GCTCCGCCTC CAACTACAAC ATATGAGACA ACTACAGCTT 
CAGAATCAGG AGTGTGTACG TGCTCAGATA AAGCGCGCAG AACAGGAGAT CGGTGTGAAA 
CTGGCTGAAA TGCTGAGACG ACAGGATGAT CCGACACAGA AACAGAGGAT GCTTGTGGAG 
GAAGAGAGAC ACCGATATCT CAGCATGCAG GAAAGAGTTC TCACACAGCT GGGGGATCTG 
GAGCAGTTTG TGGAGACTCT GAGGACTGAT TCGGGTCCTG CTGCGTCTCA GAGAGCAGTG 
ACGCTGAAGG AGGTTGAGGA TGGGGCTGTC AGCCTGAGGA AGGTGGGAGA AAACCTGGCT 
GGACTCAAAG GTGAGTTTCC GGCTCTTCAG TCCCGCATGC GCTCAGTGCT CAGGGTTGAA 
GTAGAGGCTG TAAAGTTCCT CAAAGAAGAG CCACACAAAC TGGACAGCAT GTTAAAGAGA 
GTCCGGACCC TCACTGACAC ACTCAGCAAC CTTCGCAGGT GTGCCACCGA TAACCTGCTT 
AAACCCACTG ATCAGGTTTG CTCAACACCT CCTGCCTCTG AAAAACCAGA ACTTATTGAA 
TCCCCAGAAC CTGCACCGGT CACACCTCAG AGCTCCATCG TGAGGTCAGA GGTTATGACC 
TCAAACCCTG TGGTGATCCA CCATGCTCAG GCCTCTCCAG TAGCATCACA ACAATCCCAG 
CAATCAACAG CACCTTCACC CATCCCTACA CAAAGAAGAG AGTCTACACC GTCACCCACT 
AAACAGCTCA GAAAACCCTC ACTGGAACAA ACACAAAACA ACACCAGTAA TGGAGTTTCT 
GCTAATGGCG TACATGGCAA GGGTCCTTCT CCAAACCTGA TCATTGAGGA GATCCACGCC 
AGCCACAGCA AGAACAAGAA CAGAGCTGTG TCCATAGAGG CTGCAGAGAA GGAATGGGAG 
GAGAAAAGGC AGAACTTGGG TCATTATGAT GGACAGGAGT TTGAGCGGAT TCTCCAGGAA 
GCAGAAGCAA ACATGCTGAG GGGAATACCA AACCTCGAGG TGCCCAGTCA ACTTGAGGGT 
TCTCCTGCTC CACCTGCCAT GCTTCCTGCC TCTCTGGAGG AGGTAGTAGA AATAAACGAA 
CCAGTAGCAG CACCAAGAGA ACACGATGAC AATCAGCCAA ACTCTGAGAA ACCAGCTAAG 
ACTGCTTCAG AGAAACCTCC CAAACCTCTG GAGAAACCAG TTAAGCCAGC CTTGGAGAAA 
CCTCTTAAAC CCAGCCTATC CATCAAATCC AACCTAGAGA GACAGAGTAA GACTCAGGAA 
AAAGTTAAAC TTCAGAGCAC AGAGAAAACC AACAAGTCTC CTCCTCCACC CCCACCTCGA 
CGCAACTACA CCACCAACAC CGGCATGACC ACCACCCGCT CTGGAGAGGT CATTTACACC 
AGCAGGAAAG AGTCTGTAAC GGAAGGTGAA GAGGAGTCCA ACATTTCAAC AATGTCTCAG 
CCTGAACCAA AGACCCCCCC AGAGGTAAAG CCCAAACCCA TCACCCCTCC ACCTATCTCT 
GCCTCAGCCA TCACTGAAGA AGAGGACGAA GGAGACAAGA TTATGGCAGA GCTACAGGTT 
TTCCAGAAGT GCTCTGTTAA GGAGGTAGGG CTCAAGGGTT TGGTAGAGCC ACAGATCAGA 
GAGCTAAGAC CTGGGGTCTT ACTGCCCCTT AAAGATAAGA AGAACACAGA GAACCAAGAT 
GACAAGAATA CTGACACAGA TGAGAATGGC AACAATACTA TTCGTCAAAG TCCTGGGGTC 
ATCTACTATG TCACAGCTCA GATCTCCAAG GGAACTCCAC CACCGTCAGA GGAATCAACT 
GAGCGTAGAG ATGCATCGCA GTCTTTCCCA TCACAGGTGT CACAAATCAA TGCTTCTGAC 
CAATCCCAAA GCCAGCAGCA GGTTTCCAAT AACAAATCAG ATGCCCTCAA AAACATTGGC 
CTAGCCAACT TTTCTGGCAC CCAGCAACAA AATCAGCTGT CTCGTTCAGC CTCACTAAAG 
TTACATCCAG CCTCTTCATG GGAAGAAGTT CCTGGTAGCC CAACACAAGG GAAGGTTCAT 
GTGGTCAAGG CTGCTCAAGT GCAGGTTAGC ATAGAGGAGA GGGTAGAGTC TCCAACAGCT 
GTTTGCTCAC CAGTCTCTTT TGGAGACTCT GAACCCATCC TTTCTAAAGT GATCCCTGTC 
GAGACAGCTT TGACAAATCA AAAGCTTCCA TTTGGTTCAT CCAAGGTGAA GGAAAGGAAA 
TTTGAGCAAA TGGAGGAAAA TAATGAGGCT TTTCTCAGTC CCGATTTGTC AGGAGAAGAG 
CCACCTCCTC CACCACCTGA TAACTTTGCC TTTATGATAA CCAACACCAA GGTTCAAGCC 
CTCTCCACTG GAGAGTACCA TCAGCTTGTC AACGCCAAGA AAGGAAGTGT ACAGACAGTG 
ACTTTTGGCA ACAAACAACC AAGTGCTTCT TACAGTGGCA TGGCATCAGG AGGTGATGCT 
AATACCAATG GCCCTCCTGG AGCTGATAAC ACTAGTAAAA AGCCAGTTAT CATAATCTTT 
GATGAGCCAA TGGACATCCG TTCAGCTTAC AAGCGCCTCT CTACCGTTTT CGAATGTGAG 
GAGGAGCTAG ATCGGATGCT TGCAGAAGAA AGAATAGATG AGGAGAGTGA GGAAACTGAG 
GAAGAAGAAT GGACGAGAGG AGAGAAGGTC AAACAGAATG GAGTTGAAAG ACAGTCTGTA 
AAAAGAACTC TTGCAGATGT CACAGATGGT GAAGTCAAGA ACTCTTCATC ATCCTTACTG 
GTTGCTGGTG ATGCAAAGCA AGATGGGAAG AAAAAGTTTA AGTTGAAATT TCCCAAGAAA 
CAGCTAGCAG CATTAACACA AGCTATTCGG GCTGGAAGTA AAACAGGCAA AAAGACTTTG 
CAAGTTGTCG TCTATGAGGA TGAGGAAGAG TCCGATGGCA CTGTCAAGCA ACATAAAGAG 
ACCAAGAGGT ATGAAATTGC TAGCTCCAAA ACAGAATCCT CAAAATCAAA TACACAGGAA 
TCTAATGGAC GAACTGATGA GATTCGTAAG AGCACCTACA AAACACTTGA CAGTCTAGAG 
CAAACAATAA AGCAGCTAGA GAATACAATT TGCGAAATGG GACCAACGTC TTCGGAAGAC 
ACTGAAAGCC TAACGAATTC TGAAACTCAA GAAAACAAAG TAGTGGAGAA TTCAGGCCAC 
TCAGAGAAGG GACTTTCTTC CAAAACCTTG GTTCCTAGGT TGTCTGCTGC CAGTAAGGGT 
CCTAGTCTCC GAAAGAAGAC CAAACCACAG CTCCTTCCTC GGCCTACCAA CATACCAACT 
ACAGGGGTCT CAGTAACACC AGTCGCTACC CACCAGAACG CCAACGTGGT CTCACCTACT 
AGTCGGATGC CAGTGCCAGT TTCTGTCAAG GCGCGGCAGC CATCAAGCAC CTCAGAAAAA 
GAGAGGGCAA CAAAACCGCA AAAACTACAG GACTCTCAGA GGCAGTTCCG CCAGGCTAAT 
GGAAGTGCTA AGAGATCTGG AGGAGATCCC AAATCCACTT CTCCTACCGT GCCTGCTTCT 
AAAATTCCCG CTTTTTCCAC TAGTACGGGA AAAAGCTTGT CCCAGTCTGA TTCTACTAAT 
AATGTTAATT CTTCTTCTCC TTTATCTTCT TCCCTTCCCA CCAGCAAATC CTCTATCCCT 
CCTCCTCGAT CCAGTTCCAT CCCCTCCTCC CACATCCCCT CTATTTCTAA CGGTTCTTTA 
AAATATGCCC CTCCCACTCA CAGCACTAAA GGCCTCTCTA TTCCCACACA GACACAAAAT 
GGTCGACCTT CCACTTCTTC TTCCTCCTCC TGTTCCCACT CCCCCTTGTC CCCGACTATG 
TTGAGTCAGA GTGCGAAGAG TATCCGTACC ATTCACACAC CCAGCTTCAA CAGCTACAGT 
CGGCCGCACA ACGGAAGTGC CGGTAAATCA GCTATACCAA CGGTAACTGT CGCTAAGGAT 
GCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470279.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325954.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X7, mRNA.

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CloneID	ODa151777
Clone ID Related Accession (Same CDS sequence)	XM_021470279.1
Accession Version	XM_021470279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGGT GTCACAAATC 
AATGCTTCTG ACCAATCCCA AAGCCAGCAG CAGGTTTCCA ATAACAAATC AGATGCCCTC 
AAAAACATTG GCCTAGCCAA CTTTTCTGGC ACCCAGCAAC AAAATCAGCT GTCTCGTTCA 
GCCTCACTAA AGTTACATCC AGCCTCTTCA TGGGAAGAAG TTCCTGGTAG CCCAACACAA 
GGGAAGGTTC ATGTGGTCAA GGCTGCTCAA GTGCAGGTTA GCATAGAGGA GAGGGTAGAG 
TCTCCAACAG CTGTTTGCTC ACCAGTCTCT TTTGGAGACT CTGAACCCAT CCTTTCTAAA 
GTGATCCCTG TCGAGACAGC TTTGACAAAT CAAAAGCTTC CATTTGGTTC ATCCAAGGTG 
AAGGAAAGGA AATTTGAGCA AATGGAGGAA AATAATGAGG CTTTTCTCAG TCCCGATTTG 
TCAGGAGAAG AGCCACCTCC TCCACCACCT GATAACTTTG CCTTTATGAT AACCAACACC 
AAGGTTCAAG CCCTCTCCAC TGGAGAGTAC CATCAGCTTG TCAACGCCAA GAAAGGAAGT 
GTACAGACAG TGACTTTTGG CAACAAACAA CCAAGTGCTT CTTACAGTGG CATGGCATCA 
GGAGGTGATG CTAATACCAA TGGCCCTCCT GGAGCTGATA ACACTAGTAA AAAGCCAGTT 
ATCATAATCT TTGATGAGCC AATGGACATC CGTTCAGCTT ACAAGCGCCT CTCTACCGTT 
TTCGAATGTG AGGAGGAGCT AGATCGGATG CTTGCAGAAG AAAGAATAGA TGAGGAGAGT 
GAGGAAACTG AGGAAGAAGA ATGGACGAGA GGAGAGAAGG TCAAACAGAA TGGAGTTGAA 
AGACAGTCTG TAAAAAGAAC TCTTGCAGAT GTCACAGATG GTGAAGTCAA GAACTCTTCA 
TCATCCTTAC TGGTTGCTGG TGATGCAAAG CAAGATGGGA AGAAAAAGTT TAAGTTGAAA 
TTTCCCAAGA AACAGCTAGC AGCATTAACA CAAGCTATTC GGGCTGGAAG TAAAACAGGC 
AAAAAGACTT TGCAAGTTGT CGTCTATGAG GATGAGGAAG AGTCCGATGG CACTGTCAAG 
CAACATAAAG AGACCAAGAG GTATGAAATT GCTAGCTCCA AAACAGAATC CTCAAAATCA 
AATACACAGG AATCTAATGG ACGAACTGAT GAGATTCGTA AGAGCACCTA CAAAACACTT 
GACAGTCTAG AGCAAACAAT AAAGCAGCTA GAGAATACAA TTTGCGAAAT GGGACCAACG 
TCTTCGGAAG ACACTGAAAG CCTAACGAAT TCTGAAACTC AAGAAAACAA AGTAGTGGAG 
AATTCAGGCC ACTCAGAGAA GGGACTTTCT TCCAAAACCT TGGTTCCTAG GTTGTCTGCT 
GCCAGTAAGG GTCCTAGTCT CCGAAAGAAG ACCAAACCAC AGCTCCTTCC TCGGCCTACC 
AACATACCAA CTACAGGGGT CTCAGTAACA CCAGTCGCTA CCCACCAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGACAC AAAATGGTCG ACCTTCCACT TCTTCTTCCT CCTCCTGTTC CCACTCCCCC 
TTGTCCCCGA CTATGTTGAG TCAGAGTGCG AAGAGTATCC GTACCATTCA CACACCCAGC 
TTCAACAGCT ACAGTCGGCC GCACAACGGA AGTGCCGGTA AATCAGCTAT ACCAACGGTA 
ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325954.1
CDS	599..6502
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470279.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470279.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGGT GTCACAAATC 
AATGCTTCTG ACCAATCCCA AAGCCAGCAG CAGGTTTCCA ATAACAAATC AGATGCCCTC 
AAAAACATTG GCCTAGCCAA CTTTTCTGGC ACCCAGCAAC AAAATCAGCT GTCTCGTTCA 
GCCTCACTAA AGTTACATCC AGCCTCTTCA TGGGAAGAAG TTCCTGGTAG CCCAACACAA 
GGGAAGGTTC ATGTGGTCAA GGCTGCTCAA GTGCAGGTTA GCATAGAGGA GAGGGTAGAG 
TCTCCAACAG CTGTTTGCTC ACCAGTCTCT TTTGGAGACT CTGAACCCAT CCTTTCTAAA 
GTGATCCCTG TCGAGACAGC TTTGACAAAT CAAAAGCTTC CATTTGGTTC ATCCAAGGTG 
AAGGAAAGGA AATTTGAGCA AATGGAGGAA AATAATGAGG CTTTTCTCAG TCCCGATTTG 
TCAGGAGAAG AGCCACCTCC TCCACCACCT GATAACTTTG CCTTTATGAT AACCAACACC 
AAGGTTCAAG CCCTCTCCAC TGGAGAGTAC CATCAGCTTG TCAACGCCAA GAAAGGAAGT 
GTACAGACAG TGACTTTTGG CAACAAACAA CCAAGTGCTT CTTACAGTGG CATGGCATCA 
GGAGGTGATG CTAATACCAA TGGCCCTCCT GGAGCTGATA ACACTAGTAA AAAGCCAGTT 
ATCATAATCT TTGATGAGCC AATGGACATC CGTTCAGCTT ACAAGCGCCT CTCTACCGTT 
TTCGAATGTG AGGAGGAGCT AGATCGGATG CTTGCAGAAG AAAGAATAGA TGAGGAGAGT 
GAGGAAACTG AGGAAGAAGA ATGGACGAGA GGAGAGAAGG TCAAACAGAA TGGAGTTGAA 
AGACAGTCTG TAAAAAGAAC TCTTGCAGAT GTCACAGATG GTGAAGTCAA GAACTCTTCA 
TCATCCTTAC TGGTTGCTGG TGATGCAAAG CAAGATGGGA AGAAAAAGTT TAAGTTGAAA 
TTTCCCAAGA AACAGCTAGC AGCATTAACA CAAGCTATTC GGGCTGGAAG TAAAACAGGC 
AAAAAGACTT TGCAAGTTGT CGTCTATGAG GATGAGGAAG AGTCCGATGG CACTGTCAAG 
CAACATAAAG AGACCAAGAG GTATGAAATT GCTAGCTCCA AAACAGAATC CTCAAAATCA 
AATACACAGG AATCTAATGG ACGAACTGAT GAGATTCGTA AGAGCACCTA CAAAACACTT 
GACAGTCTAG AGCAAACAAT AAAGCAGCTA GAGAATACAA TTTGCGAAAT GGGACCAACG 
TCTTCGGAAG ACACTGAAAG CCTAACGAAT TCTGAAACTC AAGAAAACAA AGTAGTGGAG 
AATTCAGGCC ACTCAGAGAA GGGACTTTCT TCCAAAACCT TGGTTCCTAG GTTGTCTGCT 
GCCAGTAAGG GTCCTAGTCT CCGAAAGAAG ACCAAACCAC AGCTCCTTCC TCGGCCTACC 
AACATACCAA CTACAGGGGT CTCAGTAACA CCAGTCGCTA CCCACCAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGACAC AAAATGGTCG ACCTTCCACT TCTTCTTCCT CCTCCTGTTC CCACTCCCCC 
TTGTCCCCGA CTATGTTGAG TCAGAGTGCG AAGAGTATCC GTACCATTCA CACACCCAGC 
TTCAACAGCT ACAGTCGGCC GCACAACGGA AGTGCCGGTA AATCAGCTAT ACCAACGGTA 
ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470280.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325955.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X8, mRNA.

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CloneID	ODa151778
Clone ID Related Accession (Same CDS sequence)	XM_021470280.1
Accession Version	XM_021470280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGATTCA TTCTGGACTG TGGCACGGCT GGAAAGATGC GAACTAAAAA GACAACAATG 
GCAGGGGTCT GTGCTAGTTG TAACCAGGTG TGCGCGGGGG GCGCTTGGAA CGGACTGCGG 
GAGTCATTTC AGAGGATGCT GTCGGCTTTG CACTCTGAGA TAGACATTCA AAGATATCTG 
CTGAAAATCC AGGCTGCTGG TTCACGCAGA ACCCGGGGCT CTAAGCAGAG TGTGGGGTGT 
CCGAGTCCAG TGCCTGTTTT TGTGGATCAG GTAGAGCACG GCTCACTGGT CTCTCTAGAT 
GCTCTGGAGG TCATGTCTGA GTGTGATATG CCCCTCGCCT TCACCCGAGG CTCCCGCTCT 
CGAGCCAGTC TGCCAGTGGT CCGCTCTACT AACCAGACCA AGGATCGCTC ACTAGGAGTG 
CTGTATCTGC AGTATGGGGA CGACACAAAG CAGATCCGCA TGCCTAATGA GATAACAAGT 
GTTGACACAA TCAGAGCCCT GTTCGTTAGT GCCTTCCCTC ATCAGCTCAA CATGAAGATG 
CTGGAGTCGC CCAGCACTGC CATCTACGTC AAAGATGAGA CCAGAAATGT GTATTATGAA 
CTCACCGATG TCAGGAGCAT CACAGATCAC TCCTGCCTGA AAGTGTATCA TAAAGACCCA 
GCACACGCCT TCAGTCACGG ACCCCGGCCG AGCAATGGAG ACACAAGGGT TCACAGGGAA 
ATGATATTTG CTGGACGGGA TGGTCACCCA CTCCGGAACC CCCCAATGGG TCCTCCACCC 
CCCCACATGC AAGGCTCCAT GTCACCCACT ACACCTCACG GGATGCCTCC CTCTCCATCT 
CGAATTCCCT TTGGCCCGCG AAACCCAGGT GGAGGTGTTG CCACAATGCC ACGTGAGCGA 
GCCGGACACA CACTCCCAGC CCCTGCCCGA GCATCCTCCC CCTGCCCGAG TGCCATCTTG 
GAGCGGCGGG ATGTCAAACC TGACGAGGAT GTAGCTGCGA AGACCATGTC TCTGAACACC 
AGAGCTGAAA GTCATTACGC CGACCCTTAT ATGTTACTTC ACCATGCCCC GCCCCCTGAC 
ACAGTAGACC ACGCCTACCA CCGTGCTACC ATGCGCTCTT ACAGCAACCC CAGCGTTCCC 
TTGGAGCCCC CAGACCATCT CTTCCGACAG AAAAGCAGGA ATAGCCAACT CCCCATGCTT 
GGCTCCAAAA CCCCGCCCCC TTCCCCGCAC CGAATGGGTG AGATGAGAAT GGTGGACATC 
CATACGGTCC AACCGCAGAG CTCCATGACT CTGGAAAGGG CTTCACCTGT TAGACAATCA 
TTTCGGAAGG AGGCTCCTCC TATGGATGCC ATGGTAAAGG CAAGAGGGGG AATGGGGTCT 
CCAGCCACCC CCGATCTTCA GGTTCACAAT CCTCTGGCGC CCCCGACAGA CCCTCAAACA 
CGAGAGAGAA TGAAGGCCAT GGAGGAGCAA ATAGCCAGTC TGACTGGTCT TGTGCAGCAC 
GCTCTCTTAA AGAGTAACAG CACTAACAGC AACAAGGAGC ACTCCAGCGA AAGACCAGTG 
AAGTCTGGAT CACCAGCTCA CAGCACATCT AGCACAGGAG GCTCTCCAGT ATTAGCACCA 
AAGGTTGTTG CTGCTCCTCC TGAGCGTAGC CCCGCCCCCA CACCACCTTT AGGACCTGCC 
CCTTTGCATG TTAACCTTCA TCTGTTCAGG AAGAATGTCT CTGAGCTCCG CCTCCAACTA 
CAACATATGA GACAACTACA GCTTCAGAAT CAGGAGTGTG TACGTGCTCA GATAAAGCGC 
GCAGAACAGG AGATCGGTGT GAAACTGGCT GAAATGCTGA GACGACAGGA TGATCCGACA 
CAGAAACAGA GGATGCTTGT GGAGGAAGAG AGACACCGAT ATCTCAGCAT GCAGGAAAGA 
GTTCTCACAC AGCTGGGGGA TCTGGAGCAG TTTGTGGAGA CTCTGAGGAC TGATTCGGGT 
CCTGCTGCGT CTCAGAGAGC AGTGACGCTG AAGGAGGTTG AGGATGGGGC TGTCAGCCTG 
AGGAAGGTGG GAGAAAACCT GGCTGGACTC AAAGGTGAGT TTCCGGCTCT TCAGTCCCGC 
ATGCGCTCAG TGCTCAGGGT TGAAGTAGAG GCTGTAAAGT TCCTCAAAGA AGAGCCACAC 
AAACTGGACA GCATGTTAAA GAGAGTCCGG ACCCTCACTG ACACACTCAG CAACCTTCGC 
AGGTGTGCCA CCGATAACCT GCTTAAACCC ACTGATCAGG TTTGCTCAAC ACCTCCTGCC 
TCTGAAAAAC CAGAACTTAT TGAATCCCCA GAACCTGCAC CGGTCACACC TCAGAGCTCC 
ATCGTGAGGT CAGAGGTTAT GACCTCAAAC CCTGTGGTGA TCCACCATGC TCAGGCCTCT 
CCAGTAGCAT CACAACAATC CCAGCAATCA ACAGCACCTT CACCCATCCC TACACAAAGA 
AGAGAGTCTA CACCGTCACC CACTAAACAG CTCAGAAAAC CCTCACTGGA ACAAACACAA 
AACAACACCA GTAATGGAGT TTCTGCTAAT GGCGTACATG GCAAGGGTCC TTCTCCAAAC 
CTGATCATTG AGGAGATCCA CGCCAGCCAC AGCAAGAACA AGAACAGAGC TGTGTCCATA 
GAGGCTGCAG AGAAGGAATG GGAGGAGAAA AGGCAGAACT TGGGTCATTA TGATGGACAG 
GAGTTTGAGC GGATTCTCCA GGAAGCAGAA GCAAACATGC TGAGGGGAAT ACCAAACCTC 
GAGGTGCCCA GTCAACTTGA GGGTTCTCCT GCTCCACCTG CCATGCTTCC TGCCTCTCTG 
GAGGAGGTAG TAGAAATAAA CGAACCAGTA GCAGCACCAA GAGAACACGA TGACAATCAG 
CCAAACTCTG AGAAACCAGC TAAGACTGCT TCAGAGAAAC CTCCCAAACC TCTGGAGAAA 
CCAGTTAAGC CAGCCTTGGA GAAACCTCTT AAACCCAGCC TATCCATCAA ATCCAACCTA 
GAGAGACAGA GTAAGACTCA GGAAAAAGTT AAACTTCAGA GCACAGAGAA AACCAACAAG 
TCTCCTCCTC CACCCCCACC TCGACGCAAC TACACCACCA ACACCGGCAT GACCACCACC 
CGCTCTGGAG AGGTCATTTA CACCAGCAGG AAAGAGTCTG TAACGGAAGG TGAAGAGGAG 
TCCAACATTT CAACAATGTC TCAGCCTGAA CCAAAGACCC CCCCAGAGGT AAAGCCCAAA 
CCCATCACCC CTCCACCTAT CTCTGCCTCA GCCATCACTG AAGAAGAGGA CGAAGGAGAC 
AAGATTATGG CAGAGCTACA GGTTTTCCAG AAGTGCTCTG TTAAGGAGGT AGGGCTCAAG 
GGTTTGGTAG AGCCACAGAT CAGAGAGCTA AGACCTGGGG TCTTACTGCC CCTTAAAGAT 
AAGAAGAACA CAGAGAACCA AGATGACAAG AATACTGACA CAGATGAGAA TGGCAACAAT 
ACTATTCGTC AAAGTCCTGG GGTCATCTAC TATGTCACAG CTCAGATCTC CAAGGGAACT 
CCACCACCGT CAGAGGAATC AACTGAGCGT AGAGATGCAT CGCAGTCTTT CCCATCACAG 
GTGTCACAAA TCAATGCTTC TGACCAATCC CAAAGCCAGC AGCAGGTTTC CAATAACAAA 
TCAGATGCCC TCAAAAACAT TGGCCTAGCC AACTTTTCTG GCACCCAGCA ACAAAATCAG 
CTGTCTCGTT CAGCCTCACT AAAGTTACAT CCAGCCTCTT CATGGGAAGA AGTTCCTGGT 
AGCCCAACAC AAGGGAAGGT TCATGTGGTC AAGGCTGCTC AAGTGCAGGT TAGCATAGAG 
GAGAGGGTAG AGTCTCCAAC AGCTGTTTGC TCACCAGTCT CTTTTGGAGA CTCTGAACCC 
ATCCTTTCTA AAGTGATCCC TGTCGAGACA GCTTTGACAA ATCAAAAGCT TCCATTTGGT 
TCATCCAAGG TGAAGGAAAG GAAATTTGAG CAAATGGAGG AAAATAATGA GGCTTTTCTC 
AGTCCCGATT TGTCAGGAGA AGAGCCACCT CCTCCACCAC CTGATAACTT TGCCTTTATG 
ATAACCAACA CCAAGGTTCA AGCCCTCTCC ACTGGAGAGT ACCATCAGCT TGTCAACGCC 
AAGAAAGGAA GTGTACAGAC AGTGACTTTT GGCAACAAAC AACCAAGTGC TTCTTACAGT 
GGCATGGCAT CAGGAGGTGA TGCTAATACC AATGGCCCTC CTGGAGCTGA TAACACTAGT 
AAAAAGCCAG TTATCATAAT CTTTGATGAG CCAATGGACA TCCGTTCAGC TTACAAGCGC 
CTCTCTACCG TTTTCGAATG TGAGGAGGAG CTAGATCGGA TGCTTGCAGA AGAAAGAATA 
GATGAGGAGA GTGAGGAAAC TGAGGAAGAA GAATGGACGA GAGGAGAGAA GGTCAAACAG 
AATGGAGTTG AAAGACAGTC TGTAAAAAGA ACTCTTGCAG ATGTCACAGA TGGTGAAGTC 
AAGAACTCTT CATCATCCTT ACTGGTTGCT GGTGATGCAA AGCAAGATGG GAAGAAAAAG 
TTTAAGTTGA AATTTCCCAA GAAACAGCTA GCAGCATTAA CACAAGCTAT TCGGGCTGGA 
AGTAAAACAG GCAAAAAGAC TTTGCAAGTT GTCGTCTATG AGGATGAGGA AGAGTCCGAT 
GGCACTGTCA AGCAACATAA AGAGACCAAG AGGTATGAAA TTGCTAGCTC CAAAACAGAA 
TCCTCAAAAT CAAATACACA GGAATCTAAT GGACGAACTG ATGAGATTCG TAAGAGCACC 
TACAAAACAC TTGACAGTCT AGAGCAAACA ATAAAGCAGC TAGAGAATAC AATTTGCGAA 
ATGGGACCAA CGTCTTCGGA AGACACTGAA AGCCTAACGA ATTCTGAAAC TCAAGAAAAC 
AAAGTAGTGG AGAATTCAGG CCACTCAGAG AAGGGACTTT CTTCCAAAAC CTTGGTTCCT 
AGGTTGTCTG CTGCCAGTAA GGGTCCTAGT CTCCGAAAGA AGACCAAACC ACAGCTCCTT 
CCTCGGCCTA CCAACATACC AACTACAGGG GTCTCAGTAA CACCAGTCGC TACCCACCAG 
AACGCCAACG TGGTCTCACC TACTAGTCGG ATGCCAGTGC CAGTTTCTGT CAAGGCGCGG 
CAGCCATCAA GCACCTCAGA AAAAGAGAGG GCAACAAAAC CGCAAAAACT ACAGGACTCT 
CAGAGGCAGT TCCGCCAGGC TAATGGAAGT GCTAAGAGAT CTGGAGGAGA TCCCAAATCC 
ACTTCTCCTA CCGTGCCTGC TTCTAAAATT CCCGCTTTTT CCACTAGTAC GGGAAAAAGC 
TTGTCCCAGT CTGATTCTAC TAATAATGTT AATTCTTCTT CTCCTTTATC TTCTTCCCTT 
CCCACCAGCA AATCCTCTAT CCCTCCTCCT CGATCCAGTT CCATCCCCTC CTCCCACATC 
CCCTCTATTT CTAACGGTTC TTTAAAATAT GCCCCTCCCA CTCACAGCAC TAAAGGCCTC 
TCTATTCCCA CACAGACACA AAATGGTCGA CCTTCCACTT CTTCTTCCTC CTCCTGTTCC 
CACTCCCCCT TGTCCCCGAC TATGTTGAGT CAGAGTGCGA AGAGTATCCG TACCATTCAC 
ACACCCAGCT TCAACAGCTA CAGTCGGCCG CACAACGGAA GTGCCGGTAA ATCAGCTATA 
CCAACGGTAA CTGTCGCTAA GGATGCTGCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325955.1
CDS	27..5759
Translation	MRFILDCGTAGKMRTKKTTMAGVCASCNQVCAGGAWNGLRESFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470280.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470280.1

ORF Insert Sequence:

ATGCGATTCA TTCTGGACTG TGGCACGGCT GGAAAGATGC GAACTAAAAA GACAACAATG 
GCAGGGGTCT GTGCTAGTTG TAACCAGGTG TGCGCGGGGG GCGCTTGGAA CGGACTGCGG 
GAGTCATTTC AGAGGATGCT GTCGGCTTTG CACTCTGAGA TAGACATTCA AAGATATCTG 
CTGAAAATCC AGGCTGCTGG TTCACGCAGA ACCCGGGGCT CTAAGCAGAG TGTGGGGTGT 
CCGAGTCCAG TGCCTGTTTT TGTGGATCAG GTAGAGCACG GCTCACTGGT CTCTCTAGAT 
GCTCTGGAGG TCATGTCTGA GTGTGATATG CCCCTCGCCT TCACCCGAGG CTCCCGCTCT 
CGAGCCAGTC TGCCAGTGGT CCGCTCTACT AACCAGACCA AGGATCGCTC ACTAGGAGTG 
CTGTATCTGC AGTATGGGGA CGACACAAAG CAGATCCGCA TGCCTAATGA GATAACAAGT 
GTTGACACAA TCAGAGCCCT GTTCGTTAGT GCCTTCCCTC ATCAGCTCAA CATGAAGATG 
CTGGAGTCGC CCAGCACTGC CATCTACGTC AAAGATGAGA CCAGAAATGT GTATTATGAA 
CTCACCGATG TCAGGAGCAT CACAGATCAC TCCTGCCTGA AAGTGTATCA TAAAGACCCA 
GCACACGCCT TCAGTCACGG ACCCCGGCCG AGCAATGGAG ACACAAGGGT TCACAGGGAA 
ATGATATTTG CTGGACGGGA TGGTCACCCA CTCCGGAACC CCCCAATGGG TCCTCCACCC 
CCCCACATGC AAGGCTCCAT GTCACCCACT ACACCTCACG GGATGCCTCC CTCTCCATCT 
CGAATTCCCT TTGGCCCGCG AAACCCAGGT GGAGGTGTTG CCACAATGCC ACGTGAGCGA 
GCCGGACACA CACTCCCAGC CCCTGCCCGA GCATCCTCCC CCTGCCCGAG TGCCATCTTG 
GAGCGGCGGG ATGTCAAACC TGACGAGGAT GTAGCTGCGA AGACCATGTC TCTGAACACC 
AGAGCTGAAA GTCATTACGC CGACCCTTAT ATGTTACTTC ACCATGCCCC GCCCCCTGAC 
ACAGTAGACC ACGCCTACCA CCGTGCTACC ATGCGCTCTT ACAGCAACCC CAGCGTTCCC 
TTGGAGCCCC CAGACCATCT CTTCCGACAG AAAAGCAGGA ATAGCCAACT CCCCATGCTT 
GGCTCCAAAA CCCCGCCCCC TTCCCCGCAC CGAATGGGTG AGATGAGAAT GGTGGACATC 
CATACGGTCC AACCGCAGAG CTCCATGACT CTGGAAAGGG CTTCACCTGT TAGACAATCA 
TTTCGGAAGG AGGCTCCTCC TATGGATGCC ATGGTAAAGG CAAGAGGGGG AATGGGGTCT 
CCAGCCACCC CCGATCTTCA GGTTCACAAT CCTCTGGCGC CCCCGACAGA CCCTCAAACA 
CGAGAGAGAA TGAAGGCCAT GGAGGAGCAA ATAGCCAGTC TGACTGGTCT TGTGCAGCAC 
GCTCTCTTAA AGAGTAACAG CACTAACAGC AACAAGGAGC ACTCCAGCGA AAGACCAGTG 
AAGTCTGGAT CACCAGCTCA CAGCACATCT AGCACAGGAG GCTCTCCAGT ATTAGCACCA 
AAGGTTGTTG CTGCTCCTCC TGAGCGTAGC CCCGCCCCCA CACCACCTTT AGGACCTGCC 
CCTTTGCATG TTAACCTTCA TCTGTTCAGG AAGAATGTCT CTGAGCTCCG CCTCCAACTA 
CAACATATGA GACAACTACA GCTTCAGAAT CAGGAGTGTG TACGTGCTCA GATAAAGCGC 
GCAGAACAGG AGATCGGTGT GAAACTGGCT GAAATGCTGA GACGACAGGA TGATCCGACA 
CAGAAACAGA GGATGCTTGT GGAGGAAGAG AGACACCGAT ATCTCAGCAT GCAGGAAAGA 
GTTCTCACAC AGCTGGGGGA TCTGGAGCAG TTTGTGGAGA CTCTGAGGAC TGATTCGGGT 
CCTGCTGCGT CTCAGAGAGC AGTGACGCTG AAGGAGGTTG AGGATGGGGC TGTCAGCCTG 
AGGAAGGTGG GAGAAAACCT GGCTGGACTC AAAGGTGAGT TTCCGGCTCT TCAGTCCCGC 
ATGCGCTCAG TGCTCAGGGT TGAAGTAGAG GCTGTAAAGT TCCTCAAAGA AGAGCCACAC 
AAACTGGACA GCATGTTAAA GAGAGTCCGG ACCCTCACTG ACACACTCAG CAACCTTCGC 
AGGTGTGCCA CCGATAACCT GCTTAAACCC ACTGATCAGG TTTGCTCAAC ACCTCCTGCC 
TCTGAAAAAC CAGAACTTAT TGAATCCCCA GAACCTGCAC CGGTCACACC TCAGAGCTCC 
ATCGTGAGGT CAGAGGTTAT GACCTCAAAC CCTGTGGTGA TCCACCATGC TCAGGCCTCT 
CCAGTAGCAT CACAACAATC CCAGCAATCA ACAGCACCTT CACCCATCCC TACACAAAGA 
AGAGAGTCTA CACCGTCACC CACTAAACAG CTCAGAAAAC CCTCACTGGA ACAAACACAA 
AACAACACCA GTAATGGAGT TTCTGCTAAT GGCGTACATG GCAAGGGTCC TTCTCCAAAC 
CTGATCATTG AGGAGATCCA CGCCAGCCAC AGCAAGAACA AGAACAGAGC TGTGTCCATA 
GAGGCTGCAG AGAAGGAATG GGAGGAGAAA AGGCAGAACT TGGGTCATTA TGATGGACAG 
GAGTTTGAGC GGATTCTCCA GGAAGCAGAA GCAAACATGC TGAGGGGAAT ACCAAACCTC 
GAGGTGCCCA GTCAACTTGA GGGTTCTCCT GCTCCACCTG CCATGCTTCC TGCCTCTCTG 
GAGGAGGTAG TAGAAATAAA CGAACCAGTA GCAGCACCAA GAGAACACGA TGACAATCAG 
CCAAACTCTG AGAAACCAGC TAAGACTGCT TCAGAGAAAC CTCCCAAACC TCTGGAGAAA 
CCAGTTAAGC CAGCCTTGGA GAAACCTCTT AAACCCAGCC TATCCATCAA ATCCAACCTA 
GAGAGACAGA GTAAGACTCA GGAAAAAGTT AAACTTCAGA GCACAGAGAA AACCAACAAG 
TCTCCTCCTC CACCCCCACC TCGACGCAAC TACACCACCA ACACCGGCAT GACCACCACC 
CGCTCTGGAG AGGTCATTTA CACCAGCAGG AAAGAGTCTG TAACGGAAGG TGAAGAGGAG 
TCCAACATTT CAACAATGTC TCAGCCTGAA CCAAAGACCC CCCCAGAGGT AAAGCCCAAA 
CCCATCACCC CTCCACCTAT CTCTGCCTCA GCCATCACTG AAGAAGAGGA CGAAGGAGAC 
AAGATTATGG CAGAGCTACA GGTTTTCCAG AAGTGCTCTG TTAAGGAGGT AGGGCTCAAG 
GGTTTGGTAG AGCCACAGAT CAGAGAGCTA AGACCTGGGG TCTTACTGCC CCTTAAAGAT 
AAGAAGAACA CAGAGAACCA AGATGACAAG AATACTGACA CAGATGAGAA TGGCAACAAT 
ACTATTCGTC AAAGTCCTGG GGTCATCTAC TATGTCACAG CTCAGATCTC CAAGGGAACT 
CCACCACCGT CAGAGGAATC AACTGAGCGT AGAGATGCAT CGCAGTCTTT CCCATCACAG 
GTGTCACAAA TCAATGCTTC TGACCAATCC CAAAGCCAGC AGCAGGTTTC CAATAACAAA 
TCAGATGCCC TCAAAAACAT TGGCCTAGCC AACTTTTCTG GCACCCAGCA ACAAAATCAG 
CTGTCTCGTT CAGCCTCACT AAAGTTACAT CCAGCCTCTT CATGGGAAGA AGTTCCTGGT 
AGCCCAACAC AAGGGAAGGT TCATGTGGTC AAGGCTGCTC AAGTGCAGGT TAGCATAGAG 
GAGAGGGTAG AGTCTCCAAC AGCTGTTTGC TCACCAGTCT CTTTTGGAGA CTCTGAACCC 
ATCCTTTCTA AAGTGATCCC TGTCGAGACA GCTTTGACAA ATCAAAAGCT TCCATTTGGT 
TCATCCAAGG TGAAGGAAAG GAAATTTGAG CAAATGGAGG AAAATAATGA GGCTTTTCTC 
AGTCCCGATT TGTCAGGAGA AGAGCCACCT CCTCCACCAC CTGATAACTT TGCCTTTATG 
ATAACCAACA CCAAGGTTCA AGCCCTCTCC ACTGGAGAGT ACCATCAGCT TGTCAACGCC 
AAGAAAGGAA GTGTACAGAC AGTGACTTTT GGCAACAAAC AACCAAGTGC TTCTTACAGT 
GGCATGGCAT CAGGAGGTGA TGCTAATACC AATGGCCCTC CTGGAGCTGA TAACACTAGT 
AAAAAGCCAG TTATCATAAT CTTTGATGAG CCAATGGACA TCCGTTCAGC TTACAAGCGC 
CTCTCTACCG TTTTCGAATG TGAGGAGGAG CTAGATCGGA TGCTTGCAGA AGAAAGAATA 
GATGAGGAGA GTGAGGAAAC TGAGGAAGAA GAATGGACGA GAGGAGAGAA GGTCAAACAG 
AATGGAGTTG AAAGACAGTC TGTAAAAAGA ACTCTTGCAG ATGTCACAGA TGGTGAAGTC 
AAGAACTCTT CATCATCCTT ACTGGTTGCT GGTGATGCAA AGCAAGATGG GAAGAAAAAG 
TTTAAGTTGA AATTTCCCAA GAAACAGCTA GCAGCATTAA CACAAGCTAT TCGGGCTGGA 
AGTAAAACAG GCAAAAAGAC TTTGCAAGTT GTCGTCTATG AGGATGAGGA AGAGTCCGAT 
GGCACTGTCA AGCAACATAA AGAGACCAAG AGGTATGAAA TTGCTAGCTC CAAAACAGAA 
TCCTCAAAAT CAAATACACA GGAATCTAAT GGACGAACTG ATGAGATTCG TAAGAGCACC 
TACAAAACAC TTGACAGTCT AGAGCAAACA ATAAAGCAGC TAGAGAATAC AATTTGCGAA 
ATGGGACCAA CGTCTTCGGA AGACACTGAA AGCCTAACGA ATTCTGAAAC TCAAGAAAAC 
AAAGTAGTGG AGAATTCAGG CCACTCAGAG AAGGGACTTT CTTCCAAAAC CTTGGTTCCT 
AGGTTGTCTG CTGCCAGTAA GGGTCCTAGT CTCCGAAAGA AGACCAAACC ACAGCTCCTT 
CCTCGGCCTA CCAACATACC AACTACAGGG GTCTCAGTAA CACCAGTCGC TACCCACCAG 
AACGCCAACG TGGTCTCACC TACTAGTCGG ATGCCAGTGC CAGTTTCTGT CAAGGCGCGG 
CAGCCATCAA GCACCTCAGA AAAAGAGAGG GCAACAAAAC CGCAAAAACT ACAGGACTCT 
CAGAGGCAGT TCCGCCAGGC TAATGGAAGT GCTAAGAGAT CTGGAGGAGA TCCCAAATCC 
ACTTCTCCTA CCGTGCCTGC TTCTAAAATT CCCGCTTTTT CCACTAGTAC GGGAAAAAGC 
TTGTCCCAGT CTGATTCTAC TAATAATGTT AATTCTTCTT CTCCTTTATC TTCTTCCCTT 
CCCACCAGCA AATCCTCTAT CCCTCCTCCT CGATCCAGTT CCATCCCCTC CTCCCACATC 
CCCTCTATTT CTAACGGTTC TTTAAAATAT GCCCCTCCCA CTCACAGCAC TAAAGGCCTC 
TCTATTCCCA CACAGACACA AAATGGTCGA CCTTCCACTT CTTCTTCCTC CTCCTGTTCC 
CACTCCCCCT TGTCCCCGAC TATGTTGAGT CAGAGTGCGA AGAGTATCCG TACCATTCAC 
ACACCCAGCT TCAACAGCTA CAGTCGGCCG CACAACGGAA GTGCCGGTAA ATCAGCTATA 
CCAACGGTAA CTGTCGCTAA GGATGCTGCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470281.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325956.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X9, mRNA.

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CloneID	ODa151779
Clone ID Related Accession (Same CDS sequence)	XM_021470281.1
Accession Version	XM_021470281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCT CGGATATGGA TAAAAAACGA GAGGCTTTTC TGGAACATCT GAAGCAGAAG 
TATCCGCACC ACGCCTCTGC CATAATGGGC CATCAGGAGA GACTCAGAGA ACAGTCATTT 
CAGAGGATGC TGTCGGCTTT GCACTCTGAG ATAGACATTC AAAGATATCT GCTGAAAATC 
CAGGCTGCTG GTTCACGCAG AACCCGGGGC TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA 
GTGCCTGTTT TTGTGGATCA GGTAGAGCAC GGCTCACTGG TCTCTCTAGA TGCTCTGGAG 
GTCATGTCTG AGTGTGATAT GCCCCTCGCC TTCACCCGAG GCTCCCGCTC TCGAGCCAGT 
CTGCCAGTGG TCCGCTCTAC TAACCAGACC AAGGATCGCT CACTAGGAGT GCTGTATCTG 
CAGTATGGGG ACGACACAAA GCAGATCCGC ATGCCTAATG AGATAACAAG TGTTGACACA 
ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT CATCAGCTCA ACATGAAGAT GCTGGAGTCG 
CCCAGCACTG CCATCTACGT CAAAGATGAG ACCAGAAATG TGTATTATGA ACTCACCGAT 
GTCAGGAGCA TCACAGATCA CTCCTGCCTG AAAGTGTATC ATAAAGACCC AGCACACGCC 
TTCAGTCACG GACCCCGGCC GAGCAATGGA GACACAAGGG TTCACAGGGA AATGATATTT 
GCTGGACGGG ATGGTCACCC ACTCCGGAAC CCCCCAATGG GTCCTCCACC CCCCCACATG 
CAAGGCTCCA TGTCACCCAC TACACCTCAC GGGATGCCTC CCTCTCCATC TCGAATTCCC 
TTTGGCCCGC GAAACCCAGG TGGAGGTGTT GCCACAATGC CACGTGAGCG AGCCGGACAC 
ACACTCCCAG CCCCTGCCCG AGCATCCTCC CCCTGCCCGA GTGCCATCTT GGAGCGGCGG 
GATGTCAAAC CTGACGAGGA TGTAGCTGCG AAGACCATGT CTCTGAACAC CAGAGCTGAA 
AGTCATTACG CCGACCCTTA TATGTTACTT CACCATGCCC CGCCCCCTGA CACAGTAGAC 
CACGCCTACC ACCGTGCTAC CATGCGCTCT TACAGCAACC CCAGCGTTCC CTTGGAGCCC 
CCAGACCATC TCTTCCGACA GAAAAGCAGG AATAGCCAAC TCCCCATGCT TGGCTCCAAA 
ACCCCGCCCC CTTCCCCGCA CCGAATGGGT GAGATGAGAA TGGTGGACAT CCATACGGTC 
CAACCGCAGA GCTCCATGAC TCTGGAAAGG GCTTCACCTG TTAGACAATC ATTTCGGAAG 
GAGGCTCCTC CTATGGATGC CATGGTAAAG GCAAGAGGGG GAATGGGGTC TCCAGCCACC 
CCCGATCTTC AGGTTCACAA TCCTCTGGCG CCCCCGACAG ACCCTCAAAC ACGAGAGAGA 
ATGAAGGCCA TGGAGGAGCA AATAGCCAGT CTGACTGGTC TTGTGCAGCA CGCTCTCTTA 
AAGAGTAACA GCACTAACAG CAACAAGGAG CACTCCAGCG AAAGACCAGT GAAGTCTGGA 
TCACCAGCTC ACAGCACATC TAGCACAGGA GGCTCTCCAG TATTAGCACC AAAGGTTGTT 
GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC ACACCACCTT TAGGACCTGC CCCTTTGCAT 
GTTAACCTTC ATCTGTTCAG GAAGAATGTC TCTGAGCTCC GCCTCCAACT ACAACATATG 
AGACAACTAC AGCTTCAGAA TCAGGAGTGT GTACGTGCTC AGATAAAGCG CGCAGAACAG 
GAGATCGGTG TGAAACTGGC TGAAATGCTG AGACGACAGG ATGATCCGAC ACAGAAACAG 
AGGATGCTTG TGGAGGAAGA GAGACACCGA TATCTCAGCA TGCAGGAAAG AGTTCTCACA 
CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG ACTCTGAGGA CTGATTCGGG TCCTGCTGCG 
TCTCAGAGAG CAGTGACGCT GAAGGAGGTT GAGGATGGGG CTGTCAGCCT GAGGAAGGTG 
GGAGAAAACC TGGCTGGACT CAAAGGTGAG TTTCCGGCTC TTCAGTCCCG CATGCGCTCA 
GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG TTCCTCAAAG AAGAGCCACA CAAACTGGAC 
AGCATGTTAA AGAGAGTCCG GACCCTCACT GACACACTCA GCAACCTTCG CAGGTGTGCC 
ACCGATAACC TGCTTAAACC CACTGATCAG GTTTGCTCAA CACCTCCTGC CTCTGAAAAA 
CCAGAACTTA TTGAATCCCC AGAACCTGCA CCGGTCACAC CTCAGAGCTC CATCGTGAGG 
TCAGAGGTTA TGACCTCAAA CCCTGTGGTG ATCCACCATG CTCAGGCCTC TCCAGTAGCA 
TCACAACAAT CCCAGCAATC AACAGCACCT TCACCCATCC CTACACAAAG AAGAGAGTCT 
ACACCGTCAC CCACTAAACA GCTCAGAAAA CCCTCACTGG AACAAACACA AAACAACACC 
AGTAATGGAG TTTCTGCTAA TGGCGTACAT GGCAAGGGTC CTTCTCCAAA CCTGATCATT 
GAGGAGATCC ACGCCAGCCA CAGCAAGAAC AAGAACAGAG CTGTGTCCAT AGAGGCTGCA 
GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC TTGGGTCATT ATGATGGACA GGAGTTTGAG 
CGGATTCTCC AGGAAGCAGA AGCAAACATG CTGAGGGGAA TACCAAACCT CGAGGTGCCC 
AGTCAACTTG AGGGTTCTCC TGCTCCACCT GCCATGCTTC CTGCCTCTCT GGAGGAGGTA 
GTAGAAATAA ACGAACCAGT AGCAGCACCA AGAGAACACG ATGACAATCA GCCAAACTCT 
GAGAAACCAG CTAAGACTGC TTCAGAGAAA CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG 
CCAGCCTTGG AGAAACCTCT TAAACCCAGC CTATCCATCA AATCCAACCT AGAGAGACAG 
AGTAAGACTC AGGAAAAAGT TAAACTTCAG AGCACAGAGA AAACCAACAA GTCTCCTCCT 
CCACCCCCAC CTCGACGCAA CTACACCACC AACACCGGCA TGACCACCAC CCGCTCTGGA 
GAGGTCATTT ACACCAGCAG GAAAGAGTCT GTAACGGAAG GTGAAGAGGA GTCCAACATT 
TCAACAATGT CTCAGCCTGA ACCAAAGACC CCCCCAGAGG TAAAGCCCAA ACCCATCACC 
CCTCCACCTA TCTCTGCCTC AGCCATCACT GAAGAAGAGG ACGAAGGAGA CAAGATTATG 
GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA 
GAGCCACAGA TCAGAGAGCT AAGACCTGGG GTCTTACTGC CCCTTAAAGA TAAGAAGAAC 
ACAGAGAACC AAGATGACAA GAATACTGAC ACAGATGAGA ATGGCAACAA TACTATTCGT 
CAAAGTCCTG GGGTCATCTA CTATGTCACA GCTCAGATCT CCAAGGGAAC TCCACCACCG 
TCAGAGGAAT CAACTGAGCG TAGAGATGCA TCGCAGTCTT TCCCATCACA GGTGTCACAA 
ATCAATGCTT CTGACCAATC CCAAAGCCAG CAGCAGGTTT CCAATAACAA ATCAGATGCC 
CTCAAAAACA TTGGCCTAGC CAACTTTTCT GGCACCCAGC AACAAAATCA GCTGTCTCGT 
TCAGCCTCAC TAAAGTTACA TCCAGCCTCT TCATGGGAAG AAGTTCCTGG TAGCCCAACA 
CAAGGGAAGG TTCATGTGGT CAAGGCTGCT CAAGTGCAGG TTAGCATAGA GGAGAGGGTA 
GAGTCTCCAA CAGCTGTTTG CTCACCAGTC TCTTTTGGAG ACTCTGAACC CATCCTTTCT 
AAAGTGATCC CTGTCGAGAC AGCTTTGACA AATCAAAAGC TTCCATTTGG TTCATCCAAG 
GTGAAGGAAA GGAAATTTGA GCAAATGGAG GAAAATAATG AGGCTTTTCT CAGTCCCGAT 
TTGTCAGGAG AAGAGCCACC TCCTCCACCA CCTGATAACT TTGCCTTTAT GATAACCAAC 
ACCAAGGTTC AAGCCCTCTC CACTGGAGAG TACCATCAGC TTGTCAACGC CAAGAAAGGA 
AGTGTACAGA CAGTGACTTT TGGCAACAAA CAACCAAGTG CTTCTTACAG TGGCATGGCA 
TCAGGAGGTG ATGCTAATAC CAATGGCCCT CCTGGAGCTG ATAACACTAG TAAAAAGCCA 
GTTATCATAA TCTTTGATGA GCCAATGGAC ATCCGTTCAG CTTACAAGCG CCTCTCTACC 
GTTTTCGAAT GTGAGGAGGA GCTAGATCGG ATGCTTGCAG AAGAAAGAAT AGATGAGGAG 
AGTGAGGAAA CTGAGGAAGA AGAATGGACG AGAGGAGAGA AGGTCAAACA GAATGGAGTT 
GAAAGACAGT CTGTAAAAAG AACTCTTGCA GATGTCACAG ATGGTGAAGT CAAGAACTCT 
TCATCATCCT TACTGGTTGC TGGTGATGCA AAGCAAGATG GGAAGAAAAA GTTTAAGTTG 
AAATTTCCCA AGAAACAGCT AGCAGCATTA ACACAAGCTA TTCGGGCTGG AAGTAAAACA 
GGCAAAAAGA CTTTGCAAGT TGTCGTCTAT GAGGATGAGG AAGAGTCCGA TGGCACTGTC 
AAGCAACATA AAGAGACCAA GAGGTATGAA ATTGCTAGCT CCAAAACAGA ATCCTCAAAA 
TCAAATACAC AGGAATCTAA TGGACGAACT GATGAGATTC GTAAGAGCAC CTACAAAACA 
CTTGACAGTC TAGAGCAAAC AATAAAGCAG CTAGAGAATA CAATTTGCGA AATGGGACCA 
ACGTCTTCGG AAGACACTGA AAGCCTAACG AATTCTGAAA CTCAAGAAAA CAAAGTAGTG 
GAGAATTCAG GCCACTCAGA GAAGGGACTT TCTTCCAAAA CCTTGGTTCC TAGGTTGTCT 
GCTGCCAGTA AGGGTCCTAG TCTCCGAAAG AAGACCAAAC CACAGCTCCT TCCTCGGCCT 
ACCAACATAC CAACTACAGG GGTCTCAGTA ACACCAGTCG CTACCCACCA GAACGCCAAC 
GTGGTCTCAC CTACTAGTCG GATGCCAGTG CCAGTTTCTG TCAAGGCGCG GCAGCCATCA 
AGCACCTCAG AAAAAGAGAG GGCAACAAAA CCGCAAAAAC TACAGGACTC TCAGAGGCAG 
TTCCGCCAGG CTAATGGAAG TGCTAAGAGA TCTGGAGGAG ATCCCAAATC CACTTCTCCT 
ACCGTGCCTG CTTCTAAAAT TCCCGCTTTT TCCACTAGTA CGGGAAAAAG CTTGTCCCAG 
TCTGATTCTA CTAATAATGT TAATTCTTCT TCTCCTTTAT CTTCTTCCCT TCCCACCAGC 
AAATCCTCTA TCCCTCCTCC TCGATCCAGT TCCATCCCCT CCTCCCACAT CCCCTCTATT 
TCTAACGGTT CTTTAAAATA TGCCCCTCCC ACTCACAGCA CTAAAGGCCT CTCTATTCCC 
ACACAGACAC AAAATGGTCG ACCTTCCACT TCTTCTTCCT CCTCCTGTTC CCACTCCCCC 
TTGTCCCCGA CTATGTTGAG TCAGAGTGCG AAGAGTATCC GTACCATTCA CACACCCAGC 
TTCAACAGCT ACAGTCGGCC GCACAACGGA AGTGCCGGTA AATCAGCTAT ACCAACGGTA 
ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325956.1
CDS	574..6297
Translation	MQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470281.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470281.1

ORF Insert Sequence:

ATGCAGCCCT CGGATATGGA TAAAAAACGA GAGGCTTTTC TGGAACATCT GAAGCAGAAG 
TATCCGCACC ACGCCTCTGC CATAATGGGC CATCAGGAGA GACTCAGAGA ACAGTCATTT 
CAGAGGATGC TGTCGGCTTT GCACTCTGAG ATAGACATTC AAAGATATCT GCTGAAAATC 
CAGGCTGCTG GTTCACGCAG AACCCGGGGC TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA 
GTGCCTGTTT TTGTGGATCA GGTAGAGCAC GGCTCACTGG TCTCTCTAGA TGCTCTGGAG 
GTCATGTCTG AGTGTGATAT GCCCCTCGCC TTCACCCGAG GCTCCCGCTC TCGAGCCAGT 
CTGCCAGTGG TCCGCTCTAC TAACCAGACC AAGGATCGCT CACTAGGAGT GCTGTATCTG 
CAGTATGGGG ACGACACAAA GCAGATCCGC ATGCCTAATG AGATAACAAG TGTTGACACA 
ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT CATCAGCTCA ACATGAAGAT GCTGGAGTCG 
CCCAGCACTG CCATCTACGT CAAAGATGAG ACCAGAAATG TGTATTATGA ACTCACCGAT 
GTCAGGAGCA TCACAGATCA CTCCTGCCTG AAAGTGTATC ATAAAGACCC AGCACACGCC 
TTCAGTCACG GACCCCGGCC GAGCAATGGA GACACAAGGG TTCACAGGGA AATGATATTT 
GCTGGACGGG ATGGTCACCC ACTCCGGAAC CCCCCAATGG GTCCTCCACC CCCCCACATG 
CAAGGCTCCA TGTCACCCAC TACACCTCAC GGGATGCCTC CCTCTCCATC TCGAATTCCC 
TTTGGCCCGC GAAACCCAGG TGGAGGTGTT GCCACAATGC CACGTGAGCG AGCCGGACAC 
ACACTCCCAG CCCCTGCCCG AGCATCCTCC CCCTGCCCGA GTGCCATCTT GGAGCGGCGG 
GATGTCAAAC CTGACGAGGA TGTAGCTGCG AAGACCATGT CTCTGAACAC CAGAGCTGAA 
AGTCATTACG CCGACCCTTA TATGTTACTT CACCATGCCC CGCCCCCTGA CACAGTAGAC 
CACGCCTACC ACCGTGCTAC CATGCGCTCT TACAGCAACC CCAGCGTTCC CTTGGAGCCC 
CCAGACCATC TCTTCCGACA GAAAAGCAGG AATAGCCAAC TCCCCATGCT TGGCTCCAAA 
ACCCCGCCCC CTTCCCCGCA CCGAATGGGT GAGATGAGAA TGGTGGACAT CCATACGGTC 
CAACCGCAGA GCTCCATGAC TCTGGAAAGG GCTTCACCTG TTAGACAATC ATTTCGGAAG 
GAGGCTCCTC CTATGGATGC CATGGTAAAG GCAAGAGGGG GAATGGGGTC TCCAGCCACC 
CCCGATCTTC AGGTTCACAA TCCTCTGGCG CCCCCGACAG ACCCTCAAAC ACGAGAGAGA 
ATGAAGGCCA TGGAGGAGCA AATAGCCAGT CTGACTGGTC TTGTGCAGCA CGCTCTCTTA 
AAGAGTAACA GCACTAACAG CAACAAGGAG CACTCCAGCG AAAGACCAGT GAAGTCTGGA 
TCACCAGCTC ACAGCACATC TAGCACAGGA GGCTCTCCAG TATTAGCACC AAAGGTTGTT 
GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC ACACCACCTT TAGGACCTGC CCCTTTGCAT 
GTTAACCTTC ATCTGTTCAG GAAGAATGTC TCTGAGCTCC GCCTCCAACT ACAACATATG 
AGACAACTAC AGCTTCAGAA TCAGGAGTGT GTACGTGCTC AGATAAAGCG CGCAGAACAG 
GAGATCGGTG TGAAACTGGC TGAAATGCTG AGACGACAGG ATGATCCGAC ACAGAAACAG 
AGGATGCTTG TGGAGGAAGA GAGACACCGA TATCTCAGCA TGCAGGAAAG AGTTCTCACA 
CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG ACTCTGAGGA CTGATTCGGG TCCTGCTGCG 
TCTCAGAGAG CAGTGACGCT GAAGGAGGTT GAGGATGGGG CTGTCAGCCT GAGGAAGGTG 
GGAGAAAACC TGGCTGGACT CAAAGGTGAG TTTCCGGCTC TTCAGTCCCG CATGCGCTCA 
GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG TTCCTCAAAG AAGAGCCACA CAAACTGGAC 
AGCATGTTAA AGAGAGTCCG GACCCTCACT GACACACTCA GCAACCTTCG CAGGTGTGCC 
ACCGATAACC TGCTTAAACC CACTGATCAG GTTTGCTCAA CACCTCCTGC CTCTGAAAAA 
CCAGAACTTA TTGAATCCCC AGAACCTGCA CCGGTCACAC CTCAGAGCTC CATCGTGAGG 
TCAGAGGTTA TGACCTCAAA CCCTGTGGTG ATCCACCATG CTCAGGCCTC TCCAGTAGCA 
TCACAACAAT CCCAGCAATC AACAGCACCT TCACCCATCC CTACACAAAG AAGAGAGTCT 
ACACCGTCAC CCACTAAACA GCTCAGAAAA CCCTCACTGG AACAAACACA AAACAACACC 
AGTAATGGAG TTTCTGCTAA TGGCGTACAT GGCAAGGGTC CTTCTCCAAA CCTGATCATT 
GAGGAGATCC ACGCCAGCCA CAGCAAGAAC AAGAACAGAG CTGTGTCCAT AGAGGCTGCA 
GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC TTGGGTCATT ATGATGGACA GGAGTTTGAG 
CGGATTCTCC AGGAAGCAGA AGCAAACATG CTGAGGGGAA TACCAAACCT CGAGGTGCCC 
AGTCAACTTG AGGGTTCTCC TGCTCCACCT GCCATGCTTC CTGCCTCTCT GGAGGAGGTA 
GTAGAAATAA ACGAACCAGT AGCAGCACCA AGAGAACACG ATGACAATCA GCCAAACTCT 
GAGAAACCAG CTAAGACTGC TTCAGAGAAA CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG 
CCAGCCTTGG AGAAACCTCT TAAACCCAGC CTATCCATCA AATCCAACCT AGAGAGACAG 
AGTAAGACTC AGGAAAAAGT TAAACTTCAG AGCACAGAGA AAACCAACAA GTCTCCTCCT 
CCACCCCCAC CTCGACGCAA CTACACCACC AACACCGGCA TGACCACCAC CCGCTCTGGA 
GAGGTCATTT ACACCAGCAG GAAAGAGTCT GTAACGGAAG GTGAAGAGGA GTCCAACATT 
TCAACAATGT CTCAGCCTGA ACCAAAGACC CCCCCAGAGG TAAAGCCCAA ACCCATCACC 
CCTCCACCTA TCTCTGCCTC AGCCATCACT GAAGAAGAGG ACGAAGGAGA CAAGATTATG 
GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA 
GAGCCACAGA TCAGAGAGCT AAGACCTGGG GTCTTACTGC CCCTTAAAGA TAAGAAGAAC 
ACAGAGAACC AAGATGACAA GAATACTGAC ACAGATGAGA ATGGCAACAA TACTATTCGT 
CAAAGTCCTG GGGTCATCTA CTATGTCACA GCTCAGATCT CCAAGGGAAC TCCACCACCG 
TCAGAGGAAT CAACTGAGCG TAGAGATGCA TCGCAGTCTT TCCCATCACA GGTGTCACAA 
ATCAATGCTT CTGACCAATC CCAAAGCCAG CAGCAGGTTT CCAATAACAA ATCAGATGCC 
CTCAAAAACA TTGGCCTAGC CAACTTTTCT GGCACCCAGC AACAAAATCA GCTGTCTCGT 
TCAGCCTCAC TAAAGTTACA TCCAGCCTCT TCATGGGAAG AAGTTCCTGG TAGCCCAACA 
CAAGGGAAGG TTCATGTGGT CAAGGCTGCT CAAGTGCAGG TTAGCATAGA GGAGAGGGTA 
GAGTCTCCAA CAGCTGTTTG CTCACCAGTC TCTTTTGGAG ACTCTGAACC CATCCTTTCT 
AAAGTGATCC CTGTCGAGAC AGCTTTGACA AATCAAAAGC TTCCATTTGG TTCATCCAAG 
GTGAAGGAAA GGAAATTTGA GCAAATGGAG GAAAATAATG AGGCTTTTCT CAGTCCCGAT 
TTGTCAGGAG AAGAGCCACC TCCTCCACCA CCTGATAACT TTGCCTTTAT GATAACCAAC 
ACCAAGGTTC AAGCCCTCTC CACTGGAGAG TACCATCAGC TTGTCAACGC CAAGAAAGGA 
AGTGTACAGA CAGTGACTTT TGGCAACAAA CAACCAAGTG CTTCTTACAG TGGCATGGCA 
TCAGGAGGTG ATGCTAATAC CAATGGCCCT CCTGGAGCTG ATAACACTAG TAAAAAGCCA 
GTTATCATAA TCTTTGATGA GCCAATGGAC ATCCGTTCAG CTTACAAGCG CCTCTCTACC 
GTTTTCGAAT GTGAGGAGGA GCTAGATCGG ATGCTTGCAG AAGAAAGAAT AGATGAGGAG 
AGTGAGGAAA CTGAGGAAGA AGAATGGACG AGAGGAGAGA AGGTCAAACA GAATGGAGTT 
GAAAGACAGT CTGTAAAAAG AACTCTTGCA GATGTCACAG ATGGTGAAGT CAAGAACTCT 
TCATCATCCT TACTGGTTGC TGGTGATGCA AAGCAAGATG GGAAGAAAAA GTTTAAGTTG 
AAATTTCCCA AGAAACAGCT AGCAGCATTA ACACAAGCTA TTCGGGCTGG AAGTAAAACA 
GGCAAAAAGA CTTTGCAAGT TGTCGTCTAT GAGGATGAGG AAGAGTCCGA TGGCACTGTC 
AAGCAACATA AAGAGACCAA GAGGTATGAA ATTGCTAGCT CCAAAACAGA ATCCTCAAAA 
TCAAATACAC AGGAATCTAA TGGACGAACT GATGAGATTC GTAAGAGCAC CTACAAAACA 
CTTGACAGTC TAGAGCAAAC AATAAAGCAG CTAGAGAATA CAATTTGCGA AATGGGACCA 
ACGTCTTCGG AAGACACTGA AAGCCTAACG AATTCTGAAA CTCAAGAAAA CAAAGTAGTG 
GAGAATTCAG GCCACTCAGA GAAGGGACTT TCTTCCAAAA CCTTGGTTCC TAGGTTGTCT 
GCTGCCAGTA AGGGTCCTAG TCTCCGAAAG AAGACCAAAC CACAGCTCCT TCCTCGGCCT 
ACCAACATAC CAACTACAGG GGTCTCAGTA ACACCAGTCG CTACCCACCA GAACGCCAAC 
GTGGTCTCAC CTACTAGTCG GATGCCAGTG CCAGTTTCTG TCAAGGCGCG GCAGCCATCA 
AGCACCTCAG AAAAAGAGAG GGCAACAAAA CCGCAAAAAC TACAGGACTC TCAGAGGCAG 
TTCCGCCAGG CTAATGGAAG TGCTAAGAGA TCTGGAGGAG ATCCCAAATC CACTTCTCCT 
ACCGTGCCTG CTTCTAAAAT TCCCGCTTTT TCCACTAGTA CGGGAAAAAG CTTGTCCCAG 
TCTGATTCTA CTAATAATGT TAATTCTTCT TCTCCTTTAT CTTCTTCCCT TCCCACCAGC 
AAATCCTCTA TCCCTCCTCC TCGATCCAGT TCCATCCCCT CCTCCCACAT CCCCTCTATT 
TCTAACGGTT CTTTAAAATA TGCCCCTCCC ACTCACAGCA CTAAAGGCCT CTCTATTCCC 
ACACAGACAC AAAATGGTCG ACCTTCCACT TCTTCTTCCT CCTCCTGTTC CCACTCCCCC 
TTGTCCCCGA CTATGTTGAG TCAGAGTGCG AAGAGTATCC GTACCATTCA CACACCCAGC 
TTCAACAGCT ACAGTCGGCC GCACAACGGA AGTGCCGGTA AATCAGCTAT ACCAACGGTA 
ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470282.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325957.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X12, mRNA.

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CloneID	ODa151780
Clone ID Related Accession (Same CDS sequence)	XM_021470282.1
Accession Version	XM_021470282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGGCTA ATGGAAGTGC TAAGAGATCT GGAGGAGATC CCAAATCCAC TTCTCCTACC 
GTGCCTGCTT CTAAAATTCC CGCTTTTTCC ACTAGTACGG GAAAAAGCTT GTCCCAGTCT 
GATTCTACTA ATAATGTTAA TTCTTCTTCT CCTTTATCTT CTTCCCTTCC CACCAGCAAA 
TCCTCTATCC CTCCTCCTCG ATCCAGTTCC ATCCCCTCCT CCCACATCCC CTCTATTTCT 
AACGGTTCTT TAAAATATGC CCCTCCCACT CACAGCACTA AAGGCCTCTC TATTCCCACA 
CAGACACAAA ATGGTCGACC TTCCACTTCT TCTTCCTCCT CCTGTTCCCA CTCCCCCTTG 
TCCCCGACTA TGTTGAGTCA GAGTGCGAAG AGTATCCGTA CCATTCACAC ACCCAGCTTC 
AACAGCTACA GTCGGCCGCA CAACGGAAGT GCCGGTAAAT CAGCTATACC AACGGTAACT 
GTCGCTAAGG ATGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325957.1
CDS	593..5293
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470282.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470282.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGGCTA ATGGAAGTGC TAAGAGATCT GGAGGAGATC CCAAATCCAC TTCTCCTACC 
GTGCCTGCTT CTAAAATTCC CGCTTTTTCC ACTAGTACGG GAAAAAGCTT GTCCCAGTCT 
GATTCTACTA ATAATGTTAA TTCTTCTTCT CCTTTATCTT CTTCCCTTCC CACCAGCAAA 
TCCTCTATCC CTCCTCCTCG ATCCAGTTCC ATCCCCTCCT CCCACATCCC CTCTATTTCT 
AACGGTTCTT TAAAATATGC CCCTCCCACT CACAGCACTA AAGGCCTCTC TATTCCCACA 
CAGACACAAA ATGGTCGACC TTCCACTTCT TCTTCCTCCT CCTGTTCCCA CTCCCCCTTG 
TCCCCGACTA TGTTGAGTCA GAGTGCGAAG AGTATCCGTA CCATTCACAC ACCCAGCTTC 
AACAGCTACA GTCGGCCGCA CAACGGAAGT GCCGGTAAAT CAGCTATACC AACGGTAACT 
GTCGCTAAGG ATGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470283.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325958.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X13, mRNA.

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CloneID	ODa151781
Clone ID Related Accession (Same CDS sequence)	XM_021470283.1
Accession Version	XM_021470283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4527bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACGCC 
AACGTGGTCT CACCTACTAG TCGGATGCCA GTGCCAGTTT CTGTCAAGGC GCGGCAGCCA 
TCAAGCACCT CAGAAAAAGA GAGGGCAACA AAACCGCAAA AACTACAGGA CTCTCAGAGG 
CAGTTCCGCC AGGCTAATGG AAGTGCTAAG AGATCTGGAG GAGATCCCAA ATCCACTTCT 
CCTACCGTGC CTGCTTCTAA AATTCCCGCT TTTTCCACTA GTACGGGAAA AAGCTTGTCC 
CAGTCTGATT CTACTAATAA TGTTAATTCT TCTTCTCCTT TATCTTCTTC CCTTCCCACC 
AGCAAATCCT CTATCCCTCC TCCTCGATCC AGTTCCATCC CCTCCTCCCA CATCCCCTCT 
ATTTCTAACG GTTCTTTAAA ATATGCCCCT CCCACTCACA GCACTAAAGG CCTCTCTATT 
CCCACACAGA CACAAAATGG TCGACCTTCC ACTTCTTCTT CCTCCTCCTG TTCCCACTCC 
CCCTTGTCCC CGACTATGTT GAGTCAGAGT GCGAAGAGTA TCCGTACCAT TCACACACCC 
AGCTTCAACA GCTACAGTCG GCCGCACAAC GGAAGTGCCG GTAAATCAGC TATACCAACG 
GTAACTGTCG CTAAGGATGC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325958.1
CDS	593..5119
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470283.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470283.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACGCC 
AACGTGGTCT CACCTACTAG TCGGATGCCA GTGCCAGTTT CTGTCAAGGC GCGGCAGCCA 
TCAAGCACCT CAGAAAAAGA GAGGGCAACA AAACCGCAAA AACTACAGGA CTCTCAGAGG 
CAGTTCCGCC AGGCTAATGG AAGTGCTAAG AGATCTGGAG GAGATCCCAA ATCCACTTCT 
CCTACCGTGC CTGCTTCTAA AATTCCCGCT TTTTCCACTA GTACGGGAAA AAGCTTGTCC 
CAGTCTGATT CTACTAATAA TGTTAATTCT TCTTCTCCTT TATCTTCTTC CCTTCCCACC 
AGCAAATCCT CTATCCCTCC TCCTCGATCC AGTTCCATCC CCTCCTCCCA CATCCCCTCT 
ATTTCTAACG GTTCTTTAAA ATATGCCCCT CCCACTCACA GCACTAAAGG CCTCTCTATT 
CCCACACAGA CACAAAATGG TCGACCTTCC ACTTCTTCTT CCTCCTCCTG TTCCCACTCC 
CCCTTGTCCC CGACTATGTT GAGTCAGAGT GCGAAGAGTA TCCGTACCAT TCACACACCC 
AGCTTCAACA GCTACAGTCG GCCGCACAAC GGAAGTGCCG GTAAATCAGC TATACCAACG 
GTAACTGTCG CTAAGGATGC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_017353919.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209408.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X4, mRNA.

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CloneID	ODa57669
Clone ID Related Accession (Same CDS sequence)	XM_017353919.1
Accession Version	XM_017353919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC ACAGAGAACC AAGATGACAA GAATACTGAC 
ACAGATGAGA ATGGCAACAA TACTATTCGT CAAAGTCCTG GGGTCATCTA CTATGTCACA 
GCTCAGATCT CCAAGGGAAC TCCACCACCG TCAGAGGAAT CAACTGAGCG TAGAGATGCA 
TCGCAGTCTT TCCCATCACA GGTGTCACAA ATCAATGCTT CTGACCAATC CCAAAGCCAG 
CAGCAGGTTT CCAATAACAA ATCAGATGCC CTCAAAAACA TTGGCCTAGC CAACTTTTCT 
GGCACCCAGC AACAAAATCA GCTGTCTCGT TCAGCCTCAC TAAAGTTACA TCCAGCCTCT 
TCATGGGAAG AAGTTCCTGG TAGCCCAACA CAAGGGAAGG TTCATGTGGT CAAGGCTGCT 
CAAGTGCAGG TTAGCATAGA GGAGAGGGTA GAGTCTCCAA CAGCTGTTTG CTCACCAGTC 
TCTTTTGGAG ACTCTGAACC CATCCTTTCT AAAGTGATCC CTGTCGAGAC AGCTTTGACA 
AATCAAAAGC TTCCATTTGG TTCATCCAAG GTGAAGGAAA GGAAATTTGA GCAAATGGAG 
GAAAATAATG AGGCTTTTCT CAGTCCCGAT TTGTCAGGAG AAGAGCCACC TCCTCCACCA 
CCTGATAACT TTGCCTTTAT GATAACCAAC ACCAAGGTTC AAGCCCTCTC CACTGGAGAG 
TACCATCAGC TTGTCAACGC CAAGAAAGGA AGTGTACAGA CAGTGACTTT TGGCAACAAA 
CAACCAAGTG CTTCTTACAG TGGCATGGCA TCAGGAGGTG ATGCTAATAC CAATGGCCCT 
CCTGGAGCTG ATAACACTAG TAAAAAGCCA GTTATCATAA TCTTTGATGA GCCAATGGAC 
ATCCGTTCAG CTTACAAGCG CCTCTCTACC GTTTTCGAAT GTGAGGAGGA GCTAGATCGG 
ATGCTTGCAG AAGAAAGAAT AGATGAGGAG AGTGAGGAAA CTGAGGAAGA AGAATGGACG 
AGAGGAGAGA AGGTCAAACA GAATGGAGTT GAAAGACAGT CTGTAAAAAG AACTCTTGCA 
GATGTCACAG ATGGTGAAGT CAAGAACTCT TCATCATCCT TACTGGTTGC TGGTGATGCA 
AAGCAAGATG GGAAGAAAAA GTTTAAGTTG AAATTTCCCA AGAAACAGCT AGCAGCATTA 
ACACAAGCTA TTCGGGCTGG AAGTAAAACA GGCAAAAAGA CTTTGCAAGT TGTCGTCTAT 
GAGGATGAGG AAGAGTCCGA TGGCACTGTC AAGCAACATA AAGAGACCAA GAGGTATGAA 
ATTGCTAGCT CCAAAACAGA ATCCTCAAAA TCAAATACAC AGGAATCTAA TGGACGAACT 
GATGAGATTC GTAAGAGCAC CTACAAAACA CTTGACAGTC TAGAGCAAAC AATAAAGCAG 
CTAGAGAATA CAATTTGCGA AATGGGACCA ACGTCTTCGG AAGACACTGA AAGCCTAACG 
AATTCTGAAA CTCAAGAAAA CAAAGTAGTG GAGAATTCAG GCCACTCAGA GAAGGGACTT 
TCTTCCAAAA CCTTGGTTCC TAGGTTGTCT GCTGCCAGTA AGGGTCCTAG TCTCCGAAAG 
AAGACCAAAC CACAGCTCCT TCCTCGGCCT ACCAACATAC CAACTACAGG GGTCTCAGTA 
ACACCAGTCG CTACCCACCA GGCTAATGGA AGTGCTAAGA GATCTGGAGG AGATCCCAAA 
TCCACTTCTC CTACCGTGCC TGCTTCTAAA ATTCCCGCTT TTTCCACTAG TACGGGAAAA 
AGCTTGTCCC AGTCTGATTC TACTAATAAT GTTAATTCTT CTTCTCCTTT ATCTTCTTCC 
CTTCCCACCA GCAAATCCTC TATCCCTCCT CCTCGATCCA GTTCCATCCC CTCCTCCCAC 
ATCCCCTCTA TTTCTAACGG TTCTTTAAAA TATGCCCCTC CCACTCACAG CACTAAAGGC 
CTCTCTATTC CCACACAGAC ACAAAATGGT CGACCTTCCA CTTCTTCTTC CTCCTCCTGT 
TCCCACTCCC CCTTGTCCCC GACTATGTTG AGTCAGAGTG CGAAGAGTAT CCGTACCATT 
CACACACCCA GCTTCAACAG CTACAGTCGG CCGCACAACG GAAGTGCCGG TAAATCAGCT 
ATACCAACGG TAACTGTCGC TAAGGATGCT GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209408.1
CDS	616..6591
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_017353919.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353919.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC ACAGAGAACC AAGATGACAA GAATACTGAC 
ACAGATGAGA ATGGCAACAA TACTATTCGT CAAAGTCCTG GGGTCATCTA CTATGTCACA 
GCTCAGATCT CCAAGGGAAC TCCACCACCG TCAGAGGAAT CAACTGAGCG TAGAGATGCA 
TCGCAGTCTT TCCCATCACA GGTGTCACAA ATCAATGCTT CTGACCAATC CCAAAGCCAG 
CAGCAGGTTT CCAATAACAA ATCAGATGCC CTCAAAAACA TTGGCCTAGC CAACTTTTCT 
GGCACCCAGC AACAAAATCA GCTGTCTCGT TCAGCCTCAC TAAAGTTACA TCCAGCCTCT 
TCATGGGAAG AAGTTCCTGG TAGCCCAACA CAAGGGAAGG TTCATGTGGT CAAGGCTGCT 
CAAGTGCAGG TTAGCATAGA GGAGAGGGTA GAGTCTCCAA CAGCTGTTTG CTCACCAGTC 
TCTTTTGGAG ACTCTGAACC CATCCTTTCT AAAGTGATCC CTGTCGAGAC AGCTTTGACA 
AATCAAAAGC TTCCATTTGG TTCATCCAAG GTGAAGGAAA GGAAATTTGA GCAAATGGAG 
GAAAATAATG AGGCTTTTCT CAGTCCCGAT TTGTCAGGAG AAGAGCCACC TCCTCCACCA 
CCTGATAACT TTGCCTTTAT GATAACCAAC ACCAAGGTTC AAGCCCTCTC CACTGGAGAG 
TACCATCAGC TTGTCAACGC CAAGAAAGGA AGTGTACAGA CAGTGACTTT TGGCAACAAA 
CAACCAAGTG CTTCTTACAG TGGCATGGCA TCAGGAGGTG ATGCTAATAC CAATGGCCCT 
CCTGGAGCTG ATAACACTAG TAAAAAGCCA GTTATCATAA TCTTTGATGA GCCAATGGAC 
ATCCGTTCAG CTTACAAGCG CCTCTCTACC GTTTTCGAAT GTGAGGAGGA GCTAGATCGG 
ATGCTTGCAG AAGAAAGAAT AGATGAGGAG AGTGAGGAAA CTGAGGAAGA AGAATGGACG 
AGAGGAGAGA AGGTCAAACA GAATGGAGTT GAAAGACAGT CTGTAAAAAG AACTCTTGCA 
GATGTCACAG ATGGTGAAGT CAAGAACTCT TCATCATCCT TACTGGTTGC TGGTGATGCA 
AAGCAAGATG GGAAGAAAAA GTTTAAGTTG AAATTTCCCA AGAAACAGCT AGCAGCATTA 
ACACAAGCTA TTCGGGCTGG AAGTAAAACA GGCAAAAAGA CTTTGCAAGT TGTCGTCTAT 
GAGGATGAGG AAGAGTCCGA TGGCACTGTC AAGCAACATA AAGAGACCAA GAGGTATGAA 
ATTGCTAGCT CCAAAACAGA ATCCTCAAAA TCAAATACAC AGGAATCTAA TGGACGAACT 
GATGAGATTC GTAAGAGCAC CTACAAAACA CTTGACAGTC TAGAGCAAAC AATAAAGCAG 
CTAGAGAATA CAATTTGCGA AATGGGACCA ACGTCTTCGG AAGACACTGA AAGCCTAACG 
AATTCTGAAA CTCAAGAAAA CAAAGTAGTG GAGAATTCAG GCCACTCAGA GAAGGGACTT 
TCTTCCAAAA CCTTGGTTCC TAGGTTGTCT GCTGCCAGTA AGGGTCCTAG TCTCCGAAAG 
AAGACCAAAC CACAGCTCCT TCCTCGGCCT ACCAACATAC CAACTACAGG GGTCTCAGTA 
ACACCAGTCG CTACCCACCA GGCTAATGGA AGTGCTAAGA GATCTGGAGG AGATCCCAAA 
TCCACTTCTC CTACCGTGCC TGCTTCTAAA ATTCCCGCTT TTTCCACTAG TACGGGAAAA 
AGCTTGTCCC AGTCTGATTC TACTAATAAT GTTAATTCTT CTTCTCCTTT ATCTTCTTCC 
CTTCCCACCA GCAAATCCTC TATCCCTCCT CCTCGATCCA GTTCCATCCC CTCCTCCCAC 
ATCCCCTCTA TTTCTAACGG TTCTTTAAAA TATGCCCCTC CCACTCACAG CACTAAAGGC 
CTCTCTATTC CCACACAGAC ACAAAATGGT CGACCTTCCA CTTCTTCTTC CTCCTCCTGT 
TCCCACTCCC CCTTGTCCCC GACTATGTTG AGTCAGAGTG CGAAGAGTAT CCGTACCATT 
CACACACCCA GCTTCAACAG CTACAGTCGG CCGCACAACG GAAGTGCCGG TAAATCAGCT 
ATACCAACGG TAACTGTCGC TAAGGATGCT GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_005162589.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162646.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X5, mRNA.

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CloneID	ODa28377
Clone ID Related Accession (Same CDS sequence)	XM_005162589.3
Accession Version	XM_005162589.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162589.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC ATGGAGACAT GAACAGGACA GACTTGCCTG GGGAAGAGCC TCTGAAGGGT 
AATCTAAAGG TGACTTCCAG TGAAGACAGC GAACACCTGA CCCGTAAACA GCAGGTTGTC 
TCTGCTAACA GCTCTGGTAA CCGCTGTGAG GCCAAATCCA GCCGCGCAGG GGTTCCACGC 
AGACACACTG TGGGTGGAGC GCGCAGCTCA CGGGAGATCC TGGCCATGCA GCCCTCGGAT 
ATGGATAAAA AACGAGAGGC TTTTCTGGAA CATCTGAAGC AGAAGTATCC GCACCACGCC 
TCTGCCATAA TGGGCCATCA GGAGAGACTC AGAGAACAGA CCCGGGGCTC TAAGCAGAGT 
GTGGGGTGTC CGAGTCCAGT GCCTGTTTTT GTGGATCAGG TAGAGCACGG CTCACTGGTC 
TCTCTAGATG CTCTGGAGGT CATGTCTGAG TGTGATATGC CCCTCGCCTT CACCCGAGGC 
TCCCGCTCTC GAGCCAGTCT GCCAGTGGTC CGCTCTACTA ACCAGACCAA GGATCGCTCA 
CTAGGAGTGC TGTATCTGCA GTATGGGGAC GACACAAAGC AGATCCGCAT GCCTAATGAG 
ATAACAAGTG TTGACACAAT CAGAGCCCTG TTCGTTAGTG CCTTCCCTCA TCAGCTCAAC 
ATGAAGATGC TGGAGTCGCC CAGCACTGCC ATCTACGTCA AAGATGAGAC CAGAAATGTG 
TATTATGAAC TCACCGATGT CAGGAGCATC ACAGATCACT CCTGCCTGAA AGTGTATCAT 
AAAGACCCAG CACACGCCTT CAGTCACGGA CCCCGGCCGA GCAATGGAGA CACAAGGGTT 
CACAGGGAAA TGATATTTGC TGGACGGGAT GGTCACCCAC TCCGGAACCC CCCAATGGGT 
CCTCCACCCC CCCACATGCA AGGCTCCATG TCACCCACTA CACCTCACGG GATGCCTCCC 
TCTCCATCTC GAATTCCCTT TGGCCCGCGA AACCCAGGTG GAGGTGTTGC CACAATGCCA 
CGTGAGCGAG CCGGACACAC ACTCCCAGCC CCTGCCCGAG CATCCTCCCC CTGCCCGAGT 
GCCATCTTGG AGCGGCGGGA TGTCAAACCT GACGAGGATG TAGCTGCGAA GACCATGTCT 
CTGAACACCA GAGCTGAAAG TCATTACGCC GACCCTTATA TGTTACTTCA CCATGCCCCG 
CCCCCTGACA CAGTAGACCA CGCCTACCAC CGTGCTACCA TGCGCTCTTA CAGCAACCCC 
AGCGTTCCCT TGGAGCCCCC AGACCATCTC TTCCGACAGA AAAGCAGGAA TAGCCAACTC 
CCCATGCTTG GCTCCAAAAC CCCGCCCCCT TCCCCGCACC GAATGGGTGA GATGAGAATG 
GTGGACATCC ATACGGTCCA ACCGCAGAGC TCCATGACTC TGGAAAGGGC TTCACCTGTT 
AGACAATCAT TTCGGAAGGA GGCTCCTCCT ATGGATGCCA TGGTAAAGGC AAGAGGGGGA 
ATGGGGTCTC CAGCCACCCC CGATCTTCAG GTTCACAATC CTCTGGCGCC CCCGACAGAC 
CCTCAAACAC GAGAGAGAAT GAAGGCCATG GAGGAGCAAA TAGCCAGTCT GACTGGTCTT 
GTGCAGCACG CTCTCTTAAA GAGTAACAGC ACTAACAGCA ACAAGGAGCA CTCCAGCGAA 
AGACCAGTGA AGTCTGGATC ACCAGCTCAC AGCACATCTA GCACAGGAGG CTCTCCAGTA 
TTAGCACCAA AGGTTGTTGC TGCTCCTCCT GAGCGTAGCC CCGCCCCCAC ACCACCTTTA 
GGACCTGCCC CTTTGCATGT TAACCTTCAT CTGTTCAGGA AGAATGTCTC TGAGCTCCGC 
CTCCAACTAC AACATATGAG ACAACTACAG CTTCAGAATC AGGAGTGTGT ACGTGCTCAG 
ATAAAGCGCG CAGAACAGGA GATCGGTGTG AAACTGGCTG AAATGCTGAG ACGACAGGAT 
GATCCGACAC AGAAACAGAG GATGCTTGTG GAGGAAGAGA GACACCGATA TCTCAGCATG 
CAGGAAAGAG TTCTCACACA GCTGGGGGAT CTGGAGCAGT TTGTGGAGAC TCTGAGGACT 
GATTCGGGTC CTGCTGCGTC TCAGAGAGCA GTGACGCTGA AGGAGGTTGA GGATGGGGCT 
GTCAGCCTGA GGAAGGTGGG AGAAAACCTG GCTGGACTCA AAGGTGAGTT TCCGGCTCTT 
CAGTCCCGCA TGCGCTCAGT GCTCAGGGTT GAAGTAGAGG CTGTAAAGTT CCTCAAAGAA 
GAGCCACACA AACTGGACAG CATGTTAAAG AGAGTCCGGA CCCTCACTGA CACACTCAGC 
AACCTTCGCA GGTGTGCCAC CGATAACCTG CTTAAACCCA CTGATCAGGT TTGCTCAACA 
CCTCCTGCCT CTGAAAAACC AGAACTTATT GAATCCCCAG AACCTGCACC GGTCACACCT 
CAGAGCTCCA TCGTGAGGTC AGAGGTTATG ACCTCAAACC CTGTGGTGAT CCACCATGCT 
CAGGCCTCTC CAGTAGCATC ACAACAATCC CAGCAATCAA CAGCACCTTC ACCCATCCCT 
ACACAAAGAA GAGAGTCTAC ACCGTCACCC ACTAAACAGC TCAGAAAACC CTCACTGGAA 
CAAACACAAA ACAACACCAG TAATGGAGTT TCTGCTAATG GCGTACATGG CAAGGGTCCT 
TCTCCAAACC TGATCATTGA GGAGATCCAC GCCAGCCACA GCAAGAACAA GAACAGAGCT 
GTGTCCATAG AGGCTGCAGA GAAGGAATGG GAGGAGAAAA GGCAGAACTT GGGTCATTAT 
GATGGACAGG AGTTTGAGCG GATTCTCCAG GAAGCAGAAG CAAACATGCT GAGGGGAATA 
CCAAACCTCG AGGTGCCCAG TCAACTTGAG GGTTCTCCTG CTCCACCTGC CATGCTTCCT 
GCCTCTCTGG AGGAGGTAGT AGAAATAAAC GAACCAGTAG CAGCACCAAG AGAACACGAT 
GACAATCAGC CAAACTCTGA GAAACCAGCT AAGACTGCTT CAGAGAAACC TCCCAAACCT 
CTGGAGAAAC CAGTTAAGCC AGCCTTGGAG AAACCTCTTA AACCCAGCCT ATCCATCAAA 
TCCAACCTAG AGAGACAGAG TAAGACTCAG GAAAAAGTTA AACTTCAGAG CACAGAGAAA 
ACCAACAAGT CTCCTCCTCC ACCCCCACCT CGACGCAACT ACACCACCAA CACCGGCATG 
ACCACCACCC GCTCTGGAGA GGTCATTTAC ACCAGCAGGA AAGAGTCTGT AACGGAAGGT 
GAAGAGGAGT CCAACATTTC AACAATGTCT CAGCCTGAAC CAAAGACCCC CCCAGAGGTA 
AAGCCCAAAC CCATCACCCC TCCACCTATC TCTGCCTCAG CCATCACTGA AGAAGAGGAC 
GAAGGAGACA AGATTATGGC AGAGCTACAG GTTTTCCAGA AGTGCTCTGT TAAGGAGGTA 
GGGCTCAAGG GTTTGGTAGA GCCACAGATC AGAGAGCTAA GACCTGGGGT CTTACTGCCC 
CTTAAAGATA AGAAGAACAC AGAGAACCAA GATGACAAGA ATACTGACAC AGATGAGAAT 
GGCAACAATA CTATTCGTCA AAGTCCTGGG GTCATCTACT ATGTCACAGC TCAGATCTCC 
AAGGGAACTC CACCACCGTC AGAGGAATCA ACTGAGCGTA GAGATGCATC GCAGTCTTTC 
CCATCACAGG TGTCACAAAT CAATGCTTCT GACCAATCCC AAAGCCAGCA GCAGGTTTCC 
AATAACAAAT CAGATGCCCT CAAAAACATT GGCCTAGCCA ACTTTTCTGG CACCCAGCAA 
CAAAATCAGC TGTCTCGTTC AGCCTCACTA AAGTTACATC CAGCCTCTTC ATGGGAAGAA 
GTTCCTGGTA GCCCAACACA AGGGAAGGTT CATGTGGTCA AGGCTGCTCA AGTGCAGGTT 
AGCATAGAGG AGAGGGTAGA GTCTCCAACA GCTGTTTGCT CACCAGTCTC TTTTGGAGAC 
TCTGAACCCA TCCTTTCTAA AGTGATCCCT GTCGAGACAG CTTTGACAAA TCAAAAGCTT 
CCATTTGGTT CATCCAAGGT GAAGGAAAGG AAATTTGAGC AAATGGAGGA AAATAATGAG 
GCTTTTCTCA GTCCCGATTT GTCAGGAGAA GAGCCACCTC CTCCACCACC TGATAACTTT 
GCCTTTATGA TAACCAACAC CAAGGTTCAA GCCCTCTCCA CTGGAGAGTA CCATCAGCTT 
GTCAACGCCA AGAAAGGAAG TGTACAGACA GTGACTTTTG GCAACAAACA ACCAAGTGCT 
TCTTACAGTG GCATGGCATC AGGAGGTGAT GCTAATACCA ATGGCCCTCC TGGAGCTGAT 
AACACTAGTA AAAAGCCAGT TATCATAATC TTTGATGAGC CAATGGACAT CCGTTCAGCT 
TACAAGCGCC TCTCTACCGT TTTCGAATGT GAGGAGGAGC TAGATCGGAT GCTTGCAGAA 
GAAAGAATAG ATGAGGAGAG TGAGGAAACT GAGGAAGAAG AATGGACGAG AGGAGAGAAG 
GTCAAACAGA ATGGAGTTGA AAGACAGTCT GTAAAAAGAA CTCTTGCAGA TGTCACAGAT 
GGTGAAGTCA AGAACTCTTC ATCATCCTTA CTGGTTGCTG GTGATGCAAA GCAAGATGGG 
AAGAAAAAGT TTAAGTTGAA ATTTCCCAAG AAACAGCTAG CAGCATTAAC ACAAGCTATT 
CGGGCTGGAA GTAAAACAGG CAAAAAGACT TTGCAAGTTG TCGTCTATGA GGATGAGGAA 
GAGTCCGATG GCACTGTCAA GCAACATAAA GAGACCAAGA GGTATGAAAT TGCTAGCTCC 
AAAACAGAAT CCTCAAAATC AAATACACAG GAATCTAATG GACGAACTGA TGAGATTCGT 
AAGAGCACCT ACAAAACACT TGACAGTCTA GAGCAAACAA TAAAGCAGCT AGAGAATACA 
ATTTGCGAAA TGGGACCAAC GTCTTCGGAA GACACTGAAA GCCTAACGAA TTCTGAAACT 
CAAGAAAACA AAGTAGTGGA GAATTCAGGC CACTCAGAGA AGGGACTTTC TTCCAAAACC 
TTGGTTCCTA GGTTGTCTGC TGCCAGTAAG GGTCCTAGTC TCCGAAAGAA GACCAAACCA 
CAGCTCCTTC CTCGGCCTAC CAACATACCA ACTACAGGGG TCTCAGTAAC ACCAGTCGCT 
ACCCACCAGA ACGCCAACGT GGTCTCACCT ACTAGTCGGA TGCCAGTGCC AGTTTCTGTC 
AAGGCGCGGC AGCCATCAAG CACCTCAGAA AAAGAGAGGG CAACAAAACC GCAAAAACTA 
CAGGACTCTC AGAGGCAGTT CCGCCAGGCT AATGGAAGTG CTAAGAGATC TGGAGGAGAT 
CCCAAATCCA CTTCTCCTAC CGTGCCTGCT TCTAAAATTC CCGCTTTTTC CACTAGTACG 
GGAAAAAGCT TGTCCCAGTC TGATTCTACT AATAATGTTA ATTCTTCTTC TCCTTTATCT 
TCTTCCCTTC CCACCAGCAA ATCCTCTATC CCTCCTCCTC GATCCAGTTC CATCCCCTCC 
TCCCACATCC CCTCTATTTC TAACGGTTCT TTAAAATATG CCCCTCCCAC TCACAGCACT 
AAAGGCCTCT CTATTCCCAC ACAGACACAA AATGGTCGAC CTTCCACTTC TTCTTCCTCC 
TCCTGTTCCC ACTCCCCCTT GTCCCCGACT ATGTTGAGTC AGAGTGCGAA GAGTATCCGT 
ACCATTCACA CACCCAGCTT CAACAGCTAC AGTCGGCCGC ACAACGGAAG TGCCGGTAAA 
TCAGCTATAC CAACGGTAAC TGTCGCTAAG GATGCTGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162646.1
CDS	218..6079
Translation	MAEHGDMNRTDLPGEEPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_005162589.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162589.3

ORF Insert Sequence:

ATGGCAGAAC ATGGAGACAT GAACAGGACA GACTTGCCTG GGGAAGAGCC TCTGAAGGGT 
AATCTAAAGG TGACTTCCAG TGAAGACAGC GAACACCTGA CCCGTAAACA GCAGGTTGTC 
TCTGCTAACA GCTCTGGTAA CCGCTGTGAG GCCAAATCCA GCCGCGCAGG GGTTCCACGC 
AGACACACTG TGGGTGGAGC GCGCAGCTCA CGGGAGATCC TGGCCATGCA GCCCTCGGAT 
ATGGATAAAA AACGAGAGGC TTTTCTGGAA CATCTGAAGC AGAAGTATCC GCACCACGCC 
TCTGCCATAA TGGGCCATCA GGAGAGACTC AGAGAACAGA CCCGGGGCTC TAAGCAGAGT 
GTGGGGTGTC CGAGTCCAGT GCCTGTTTTT GTGGATCAGG TAGAGCACGG CTCACTGGTC 
TCTCTAGATG CTCTGGAGGT CATGTCTGAG TGTGATATGC CCCTCGCCTT CACCCGAGGC 
TCCCGCTCTC GAGCCAGTCT GCCAGTGGTC CGCTCTACTA ACCAGACCAA GGATCGCTCA 
CTAGGAGTGC TGTATCTGCA GTATGGGGAC GACACAAAGC AGATCCGCAT GCCTAATGAG 
ATAACAAGTG TTGACACAAT CAGAGCCCTG TTCGTTAGTG CCTTCCCTCA TCAGCTCAAC 
ATGAAGATGC TGGAGTCGCC CAGCACTGCC ATCTACGTCA AAGATGAGAC CAGAAATGTG 
TATTATGAAC TCACCGATGT CAGGAGCATC ACAGATCACT CCTGCCTGAA AGTGTATCAT 
AAAGACCCAG CACACGCCTT CAGTCACGGA CCCCGGCCGA GCAATGGAGA CACAAGGGTT 
CACAGGGAAA TGATATTTGC TGGACGGGAT GGTCACCCAC TCCGGAACCC CCCAATGGGT 
CCTCCACCCC CCCACATGCA AGGCTCCATG TCACCCACTA CACCTCACGG GATGCCTCCC 
TCTCCATCTC GAATTCCCTT TGGCCCGCGA AACCCAGGTG GAGGTGTTGC CACAATGCCA 
CGTGAGCGAG CCGGACACAC ACTCCCAGCC CCTGCCCGAG CATCCTCCCC CTGCCCGAGT 
GCCATCTTGG AGCGGCGGGA TGTCAAACCT GACGAGGATG TAGCTGCGAA GACCATGTCT 
CTGAACACCA GAGCTGAAAG TCATTACGCC GACCCTTATA TGTTACTTCA CCATGCCCCG 
CCCCCTGACA CAGTAGACCA CGCCTACCAC CGTGCTACCA TGCGCTCTTA CAGCAACCCC 
AGCGTTCCCT TGGAGCCCCC AGACCATCTC TTCCGACAGA AAAGCAGGAA TAGCCAACTC 
CCCATGCTTG GCTCCAAAAC CCCGCCCCCT TCCCCGCACC GAATGGGTGA GATGAGAATG 
GTGGACATCC ATACGGTCCA ACCGCAGAGC TCCATGACTC TGGAAAGGGC TTCACCTGTT 
AGACAATCAT TTCGGAAGGA GGCTCCTCCT ATGGATGCCA TGGTAAAGGC AAGAGGGGGA 
ATGGGGTCTC CAGCCACCCC CGATCTTCAG GTTCACAATC CTCTGGCGCC CCCGACAGAC 
CCTCAAACAC GAGAGAGAAT GAAGGCCATG GAGGAGCAAA TAGCCAGTCT GACTGGTCTT 
GTGCAGCACG CTCTCTTAAA GAGTAACAGC ACTAACAGCA ACAAGGAGCA CTCCAGCGAA 
AGACCAGTGA AGTCTGGATC ACCAGCTCAC AGCACATCTA GCACAGGAGG CTCTCCAGTA 
TTAGCACCAA AGGTTGTTGC TGCTCCTCCT GAGCGTAGCC CCGCCCCCAC ACCACCTTTA 
GGACCTGCCC CTTTGCATGT TAACCTTCAT CTGTTCAGGA AGAATGTCTC TGAGCTCCGC 
CTCCAACTAC AACATATGAG ACAACTACAG CTTCAGAATC AGGAGTGTGT ACGTGCTCAG 
ATAAAGCGCG CAGAACAGGA GATCGGTGTG AAACTGGCTG AAATGCTGAG ACGACAGGAT 
GATCCGACAC AGAAACAGAG GATGCTTGTG GAGGAAGAGA GACACCGATA TCTCAGCATG 
CAGGAAAGAG TTCTCACACA GCTGGGGGAT CTGGAGCAGT TTGTGGAGAC TCTGAGGACT 
GATTCGGGTC CTGCTGCGTC TCAGAGAGCA GTGACGCTGA AGGAGGTTGA GGATGGGGCT 
GTCAGCCTGA GGAAGGTGGG AGAAAACCTG GCTGGACTCA AAGGTGAGTT TCCGGCTCTT 
CAGTCCCGCA TGCGCTCAGT GCTCAGGGTT GAAGTAGAGG CTGTAAAGTT CCTCAAAGAA 
GAGCCACACA AACTGGACAG CATGTTAAAG AGAGTCCGGA CCCTCACTGA CACACTCAGC 
AACCTTCGCA GGTGTGCCAC CGATAACCTG CTTAAACCCA CTGATCAGGT TTGCTCAACA 
CCTCCTGCCT CTGAAAAACC AGAACTTATT GAATCCCCAG AACCTGCACC GGTCACACCT 
CAGAGCTCCA TCGTGAGGTC AGAGGTTATG ACCTCAAACC CTGTGGTGAT CCACCATGCT 
CAGGCCTCTC CAGTAGCATC ACAACAATCC CAGCAATCAA CAGCACCTTC ACCCATCCCT 
ACACAAAGAA GAGAGTCTAC ACCGTCACCC ACTAAACAGC TCAGAAAACC CTCACTGGAA 
CAAACACAAA ACAACACCAG TAATGGAGTT TCTGCTAATG GCGTACATGG CAAGGGTCCT 
TCTCCAAACC TGATCATTGA GGAGATCCAC GCCAGCCACA GCAAGAACAA GAACAGAGCT 
GTGTCCATAG AGGCTGCAGA GAAGGAATGG GAGGAGAAAA GGCAGAACTT GGGTCATTAT 
GATGGACAGG AGTTTGAGCG GATTCTCCAG GAAGCAGAAG CAAACATGCT GAGGGGAATA 
CCAAACCTCG AGGTGCCCAG TCAACTTGAG GGTTCTCCTG CTCCACCTGC CATGCTTCCT 
GCCTCTCTGG AGGAGGTAGT AGAAATAAAC GAACCAGTAG CAGCACCAAG AGAACACGAT 
GACAATCAGC CAAACTCTGA GAAACCAGCT AAGACTGCTT CAGAGAAACC TCCCAAACCT 
CTGGAGAAAC CAGTTAAGCC AGCCTTGGAG AAACCTCTTA AACCCAGCCT ATCCATCAAA 
TCCAACCTAG AGAGACAGAG TAAGACTCAG GAAAAAGTTA AACTTCAGAG CACAGAGAAA 
ACCAACAAGT CTCCTCCTCC ACCCCCACCT CGACGCAACT ACACCACCAA CACCGGCATG 
ACCACCACCC GCTCTGGAGA GGTCATTTAC ACCAGCAGGA AAGAGTCTGT AACGGAAGGT 
GAAGAGGAGT CCAACATTTC AACAATGTCT CAGCCTGAAC CAAAGACCCC CCCAGAGGTA 
AAGCCCAAAC CCATCACCCC TCCACCTATC TCTGCCTCAG CCATCACTGA AGAAGAGGAC 
GAAGGAGACA AGATTATGGC AGAGCTACAG GTTTTCCAGA AGTGCTCTGT TAAGGAGGTA 
GGGCTCAAGG GTTTGGTAGA GCCACAGATC AGAGAGCTAA GACCTGGGGT CTTACTGCCC 
CTTAAAGATA AGAAGAACAC AGAGAACCAA GATGACAAGA ATACTGACAC AGATGAGAAT 
GGCAACAATA CTATTCGTCA AAGTCCTGGG GTCATCTACT ATGTCACAGC TCAGATCTCC 
AAGGGAACTC CACCACCGTC AGAGGAATCA ACTGAGCGTA GAGATGCATC GCAGTCTTTC 
CCATCACAGG TGTCACAAAT CAATGCTTCT GACCAATCCC AAAGCCAGCA GCAGGTTTCC 
AATAACAAAT CAGATGCCCT CAAAAACATT GGCCTAGCCA ACTTTTCTGG CACCCAGCAA 
CAAAATCAGC TGTCTCGTTC AGCCTCACTA AAGTTACATC CAGCCTCTTC ATGGGAAGAA 
GTTCCTGGTA GCCCAACACA AGGGAAGGTT CATGTGGTCA AGGCTGCTCA AGTGCAGGTT 
AGCATAGAGG AGAGGGTAGA GTCTCCAACA GCTGTTTGCT CACCAGTCTC TTTTGGAGAC 
TCTGAACCCA TCCTTTCTAA AGTGATCCCT GTCGAGACAG CTTTGACAAA TCAAAAGCTT 
CCATTTGGTT CATCCAAGGT GAAGGAAAGG AAATTTGAGC AAATGGAGGA AAATAATGAG 
GCTTTTCTCA GTCCCGATTT GTCAGGAGAA GAGCCACCTC CTCCACCACC TGATAACTTT 
GCCTTTATGA TAACCAACAC CAAGGTTCAA GCCCTCTCCA CTGGAGAGTA CCATCAGCTT 
GTCAACGCCA AGAAAGGAAG TGTACAGACA GTGACTTTTG GCAACAAACA ACCAAGTGCT 
TCTTACAGTG GCATGGCATC AGGAGGTGAT GCTAATACCA ATGGCCCTCC TGGAGCTGAT 
AACACTAGTA AAAAGCCAGT TATCATAATC TTTGATGAGC CAATGGACAT CCGTTCAGCT 
TACAAGCGCC TCTCTACCGT TTTCGAATGT GAGGAGGAGC TAGATCGGAT GCTTGCAGAA 
GAAAGAATAG ATGAGGAGAG TGAGGAAACT GAGGAAGAAG AATGGACGAG AGGAGAGAAG 
GTCAAACAGA ATGGAGTTGA AAGACAGTCT GTAAAAAGAA CTCTTGCAGA TGTCACAGAT 
GGTGAAGTCA AGAACTCTTC ATCATCCTTA CTGGTTGCTG GTGATGCAAA GCAAGATGGG 
AAGAAAAAGT TTAAGTTGAA ATTTCCCAAG AAACAGCTAG CAGCATTAAC ACAAGCTATT 
CGGGCTGGAA GTAAAACAGG CAAAAAGACT TTGCAAGTTG TCGTCTATGA GGATGAGGAA 
GAGTCCGATG GCACTGTCAA GCAACATAAA GAGACCAAGA GGTATGAAAT TGCTAGCTCC 
AAAACAGAAT CCTCAAAATC AAATACACAG GAATCTAATG GACGAACTGA TGAGATTCGT 
AAGAGCACCT ACAAAACACT TGACAGTCTA GAGCAAACAA TAAAGCAGCT AGAGAATACA 
ATTTGCGAAA TGGGACCAAC GTCTTCGGAA GACACTGAAA GCCTAACGAA TTCTGAAACT 
CAAGAAAACA AAGTAGTGGA GAATTCAGGC CACTCAGAGA AGGGACTTTC TTCCAAAACC 
TTGGTTCCTA GGTTGTCTGC TGCCAGTAAG GGTCCTAGTC TCCGAAAGAA GACCAAACCA 
CAGCTCCTTC CTCGGCCTAC CAACATACCA ACTACAGGGG TCTCAGTAAC ACCAGTCGCT 
ACCCACCAGA ACGCCAACGT GGTCTCACCT ACTAGTCGGA TGCCAGTGCC AGTTTCTGTC 
AAGGCGCGGC AGCCATCAAG CACCTCAGAA AAAGAGAGGG CAACAAAACC GCAAAAACTA 
CAGGACTCTC AGAGGCAGTT CCGCCAGGCT AATGGAAGTG CTAAGAGATC TGGAGGAGAT 
CCCAAATCCA CTTCTCCTAC CGTGCCTGCT TCTAAAATTC CCGCTTTTTC CACTAGTACG 
GGAAAAAGCT TGTCCCAGTC TGATTCTACT AATAATGTTA ATTCTTCTTC TCCTTTATCT 
TCTTCCCTTC CCACCAGCAA ATCCTCTATC CCTCCTCCTC GATCCAGTTC CATCCCCTCC 
TCCCACATCC CCTCTATTTC TAACGGTTCT TTAAAATATG CCCCTCCCAC TCACAGCACT 
AAAGGCCTCT CTATTCCCAC ACAGACACAA AATGGTCGAC CTTCCACTTC TTCTTCCTCC 
TCCTGTTCCC ACTCCCCCTT GTCCCCGACT ATGTTGAGTC AGAGTGCGAA GAGTATCCGT 
ACCATTCACA CACCCAGCTT CAACAGCTAC AGTCGGCCGC ACAACGGAAG TGCCGGTAAA 
TCAGCTATAC CAACGGTAAC TGTCGCTAAG GATGCTGCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_005162590.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162647.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X6, mRNA.

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CloneID	ODa28378
Clone ID Related Accession (Same CDS sequence)	XM_005162590.3
Accession Version	XM_005162590.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162590.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCT CGGATATGGA TAAAAAACGA GAGGCTTTTC TGGAACATCT GAAGCAGAAG 
TATCCGCACC ACGCCTCTGC CATAATGGGC CATCAGGAGA GACTCAGAGA ACAGACCCGG 
GGCTCTAAGC AGAGTGTGGG GTGTCCGAGT CCAGTGCCTG TTTTTGTGGA TCAGGTAGAG 
CACGGCTCAC TGGTCTCTCT AGATGCTCTG GAGGTCATGT CTGAGTGTGA TATGCCCCTC 
GCCTTCACCC GAGGCTCCCG CTCTCGAGCC AGTCTGCCAG TGGTCCGCTC TACTAACCAG 
ACCAAGGATC GCTCACTAGG AGTGCTGTAT CTGCAGTATG GGGACGACAC AAAGCAGATC 
CGCATGCCTA ATGAGATAAC AAGTGTTGAC ACAATCAGAG CCCTGTTCGT TAGTGCCTTC 
CCTCATCAGC TCAACATGAA GATGCTGGAG TCGCCCAGCA CTGCCATCTA CGTCAAAGAT 
GAGACCAGAA ATGTGTATTA TGAACTCACC GATGTCAGGA GCATCACAGA TCACTCCTGC 
CTGAAAGTGT ATCATAAAGA CCCAGCACAC GCCTTCAGTC ACGGACCCCG GCCGAGCAAT 
GGAGACACAA GGGTTCACAG GGAAATGATA TTTGCTGGAC GGGATGGTCA CCCACTCCGG 
AACCCCCCAA TGGGTCCTCC ACCCCCCCAC ATGCAAGGCT CCATGTCACC CACTACACCT 
CACGGGATGC CTCCCTCTCC ATCTCGAATT CCCTTTGGCC CGCGAAACCC AGGTGGAGGT 
GTTGCCACAA TGCCACGTGA GCGAGCCGGA CACACACTCC CAGCCCCTGC CCGAGCATCC 
TCCCCCTGCC CGAGTGCCAT CTTGGAGCGG CGGGATGTCA AACCTGACGA GGATGTAGCT 
GCGAAGACCA TGTCTCTGAA CACCAGAGCT GAAAGTCATT ACGCCGACCC TTATATGTTA 
CTTCACCATG CCCCGCCCCC TGACACAGTA GACCACGCCT ACCACCGTGC TACCATGCGC 
TCTTACAGCA ACCCCAGCGT TCCCTTGGAG CCCCCAGACC ATCTCTTCCG ACAGAAAAGC 
AGGAATAGCC AACTCCCCAT GCTTGGCTCC AAAACCCCGC CCCCTTCCCC GCACCGAATG 
GGTGAGATGA GAATGGTGGA CATCCATACG GTCCAACCGC AGAGCTCCAT GACTCTGGAA 
AGGGCTTCAC CTGTTAGACA ATCATTTCGG AAGGAGGCTC CTCCTATGGA TGCCATGGTA 
AAGGCAAGAG GGGGAATGGG GTCTCCAGCC ACCCCCGATC TTCAGGTTCA CAATCCTCTG 
GCGCCCCCGA CAGACCCTCA AACACGAGAG AGAATGAAGG CCATGGAGGA GCAAATAGCC 
AGTCTGACTG GTCTTGTGCA GCACGCTCTC TTAAAGAGTA ACAGCACTAA CAGCAACAAG 
GAGCACTCCA GCGAAAGACC AGTGAAGTCT GGATCACCAG CTCACAGCAC ATCTAGCACA 
GGAGGCTCTC CAGTATTAGC ACCAAAGGTT GTTGCTGCTC CTCCTGAGCG TAGCCCCGCC 
CCCACACCAC CTTTAGGACC TGCCCCTTTG CATGTTAACC TTCATCTGTT CAGGAAGAAT 
GTCTCTGAGC TCCGCCTCCA ACTACAACAT ATGAGACAAC TACAGCTTCA GAATCAGGAG 
TGTGTACGTG CTCAGATAAA GCGCGCAGAA CAGGAGATCG GTGTGAAACT GGCTGAAATG 
CTGAGACGAC AGGATGATCC GACACAGAAA CAGAGGATGC TTGTGGAGGA AGAGAGACAC 
CGATATCTCA GCATGCAGGA AAGAGTTCTC ACACAGCTGG GGGATCTGGA GCAGTTTGTG 
GAGACTCTGA GGACTGATTC GGGTCCTGCT GCGTCTCAGA GAGCAGTGAC GCTGAAGGAG 
GTTGAGGATG GGGCTGTCAG CCTGAGGAAG GTGGGAGAAA ACCTGGCTGG ACTCAAAGGT 
GAGTTTCCGG CTCTTCAGTC CCGCATGCGC TCAGTGCTCA GGGTTGAAGT AGAGGCTGTA 
AAGTTCCTCA AAGAAGAGCC ACACAAACTG GACAGCATGT TAAAGAGAGT CCGGACCCTC 
ACTGACACAC TCAGCAACCT TCGCAGGTGT GCCACCGATA ACCTGCTTAA ACCCACTGAT 
CAGGTTTGCT CAACACCTCC TGCCTCTGAA AAACCAGAAC TTATTGAATC CCCAGAACCT 
GCACCGGTCA CACCTCAGAG CTCCATCGTG AGGTCAGAGG TTATGACCTC AAACCCTGTG 
GTGATCCACC ATGCTCAGGC CTCTCCAGTA GCATCACAAC AATCCCAGCA ATCAACAGCA 
CCTTCACCCA TCCCTACACA AAGAAGAGAG TCTACACCGT CACCCACTAA ACAGCTCAGA 
AAACCCTCAC TGGAACAAAC ACAAAACAAC ACCAGTAATG GAGTTTCTGC TAATGGCGTA 
CATGGCAAGG GTCCTTCTCC AAACCTGATC ATTGAGGAGA TCCACGCCAG CCACAGCAAG 
AACAAGAACA GAGCTGTGTC CATAGAGGCT GCAGAGAAGG AATGGGAGGA GAAAAGGCAG 
AACTTGGGTC ATTATGATGG ACAGGAGTTT GAGCGGATTC TCCAGGAAGC AGAAGCAAAC 
ATGCTGAGGG GAATACCAAA CCTCGAGGTG CCCAGTCAAC TTGAGGGTTC TCCTGCTCCA 
CCTGCCATGC TTCCTGCCTC TCTGGAGGAG GTAGTAGAAA TAAACGAACC AGTAGCAGCA 
CCAAGAGAAC ACGATGACAA TCAGCCAAAC TCTGAGAAAC CAGCTAAGAC TGCTTCAGAG 
AAACCTCCCA AACCTCTGGA GAAACCAGTT AAGCCAGCCT TGGAGAAACC TCTTAAACCC 
AGCCTATCCA TCAAATCCAA CCTAGAGAGA CAGAGTAAGA CTCAGGAAAA AGTTAAACTT 
CAGAGCACAG AGAAAACCAA CAAGTCTCCT CCTCCACCCC CACCTCGACG CAACTACACC 
ACCAACACCG GCATGACCAC CACCCGCTCT GGAGAGGTCA TTTACACCAG CAGGAAAGAG 
TCTGTAACGG AAGGTGAAGA GGAGTCCAAC ATTTCAACAA TGTCTCAGCC TGAACCAAAG 
ACCCCCCCAG AGGTAAAGCC CAAACCCATC ACCCCTCCAC CTATCTCTGC CTCAGCCATC 
ACTGAAGAAG AGGACGAAGG AGACAAGATT ATGGCAGAGC TACAGGTTTT CCAGAAGTGC 
TCTGTTAAGG AGGTAGGGCT CAAGGGTTTG GTAGAGCCAC AGATCAGAGA GCTAAGACCT 
GGGGTCTTAC TGCCCCTTAA AGATAAGAAG AACACAGAGA ACCAAGATGA CAAGAATACT 
GACACAGATG AGAATGGCAA CAATACTATT CGTCAAAGTC CTGGGGTCAT CTACTATGTC 
ACAGCTCAGA TCTCCAAGGG AACTCCACCA CCGTCAGAGG AATCAACTGA GCGTAGAGAT 
GCATCGCAGT CTTTCCCATC ACAGGTGTCA CAAATCAATG CTTCTGACCA ATCCCAAAGC 
CAGCAGCAGG TTTCCAATAA CAAATCAGAT GCCCTCAAAA ACATTGGCCT AGCCAACTTT 
TCTGGCACCC AGCAACAAAA TCAGCTGTCT CGTTCAGCCT CACTAAAGTT ACATCCAGCC 
TCTTCATGGG AAGAAGTTCC TGGTAGCCCA ACACAAGGGA AGGTTCATGT GGTCAAGGCT 
GCTCAAGTGC AGGTTAGCAT AGAGGAGAGG GTAGAGTCTC CAACAGCTGT TTGCTCACCA 
GTCTCTTTTG GAGACTCTGA ACCCATCCTT TCTAAAGTGA TCCCTGTCGA GACAGCTTTG 
ACAAATCAAA AGCTTCCATT TGGTTCATCC AAGGTGAAGG AAAGGAAATT TGAGCAAATG 
GAGGAAAATA ATGAGGCTTT TCTCAGTCCC GATTTGTCAG GAGAAGAGCC ACCTCCTCCA 
CCACCTGATA ACTTTGCCTT TATGATAACC AACACCAAGG TTCAAGCCCT CTCCACTGGA 
GAGTACCATC AGCTTGTCAA CGCCAAGAAA GGAAGTGTAC AGACAGTGAC TTTTGGCAAC 
AAACAACCAA GTGCTTCTTA CAGTGGCATG GCATCAGGAG GTGATGCTAA TACCAATGGC 
CCTCCTGGAG CTGATAACAC TAGTAAAAAG CCAGTTATCA TAATCTTTGA TGAGCCAATG 
GACATCCGTT CAGCTTACAA GCGCCTCTCT ACCGTTTTCG AATGTGAGGA GGAGCTAGAT 
CGGATGCTTG CAGAAGAAAG AATAGATGAG GAGAGTGAGG AAACTGAGGA AGAAGAATGG 
ACGAGAGGAG AGAAGGTCAA ACAGAATGGA GTTGAAAGAC AGTCTGTAAA AAGAACTCTT 
GCAGATGTCA CAGATGGTGA AGTCAAGAAC TCTTCATCAT CCTTACTGGT TGCTGGTGAT 
GCAAAGCAAG ATGGGAAGAA AAAGTTTAAG TTGAAATTTC CCAAGAAACA GCTAGCAGCA 
TTAACACAAG CTATTCGGGC TGGAAGTAAA ACAGGCAAAA AGACTTTGCA AGTTGTCGTC 
TATGAGGATG AGGAAGAGTC CGATGGCACT GTCAAGCAAC ATAAAGAGAC CAAGAGGTAT 
GAAATTGCTA GCTCCAAAAC AGAATCCTCA AAATCAAATA CACAGGAATC TAATGGACGA 
ACTGATGAGA TTCGTAAGAG CACCTACAAA ACACTTGACA GTCTAGAGCA AACAATAAAG 
CAGCTAGAGA ATACAATTTG CGAAATGGGA CCAACGTCTT CGGAAGACAC TGAAAGCCTA 
ACGAATTCTG AAACTCAAGA AAACAAAGTA GTGGAGAATT CAGGCCACTC AGAGAAGGGA 
CTTTCTTCCA AAACCTTGGT TCCTAGGTTG TCTGCTGCCA GTAAGGGTCC TAGTCTCCGA 
AAGAAGACCA AACCACAGCT CCTTCCTCGG CCTACCAACA TACCAACTAC AGGGGTCTCA 
GTAACACCAG TCGCTACCCA CCAGAACGCC AACGTGGTCT CACCTACTAG TCGGATGCCA 
GTGCCAGTTT CTGTCAAGGC GCGGCAGCCA TCAAGCACCT CAGAAAAAGA GAGGGCAACA 
AAACCGCAAA AACTACAGGA CTCTCAGAGG CAGTTCCGCC AGGCTAATGG AAGTGCTAAG 
AGATCTGGAG GAGATCCCAA ATCCACTTCT CCTACCGTGC CTGCTTCTAA AATTCCCGCT 
TTTTCCACTA GTACGGGAAA AAGCTTGTCC CAGTCTGATT CTACTAATAA TGTTAATTCT 
TCTTCTCCTT TATCTTCTTC CCTTCCCACC AGCAAATCCT CTATCCCTCC TCCTCGATCC 
AGTTCCATCC CCTCCTCCCA CATCCCCTCT ATTTCTAACG GTTCTTTAAA ATATGCCCCT 
CCCACTCACA GCACTAAAGG CCTCTCTATT CCCACACAGA CACAAAATGG TCGACCTTCC 
ACTTCTTCTT CCTCCTCCTG TTCCCACTCC CCCTTGTCCC CGACTATGTT GAGTCAGAGT 
GCGAAGAGTA TCCGTACCAT TCACACACCC AGCTTCAACA GCTACAGTCG GCCGCACAAC 
GGAAGTGCCG GTAAATCAGC TATACCAACG GTAACTGTCG CTAAGGATGC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162647.1
CDS	591..6227
Translation	MQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_005162590.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162590.3

ORF Insert Sequence:

ATGCAGCCCT CGGATATGGA TAAAAAACGA GAGGCTTTTC TGGAACATCT GAAGCAGAAG 
TATCCGCACC ACGCCTCTGC CATAATGGGC CATCAGGAGA GACTCAGAGA ACAGACCCGG 
GGCTCTAAGC AGAGTGTGGG GTGTCCGAGT CCAGTGCCTG TTTTTGTGGA TCAGGTAGAG 
CACGGCTCAC TGGTCTCTCT AGATGCTCTG GAGGTCATGT CTGAGTGTGA TATGCCCCTC 
GCCTTCACCC GAGGCTCCCG CTCTCGAGCC AGTCTGCCAG TGGTCCGCTC TACTAACCAG 
ACCAAGGATC GCTCACTAGG AGTGCTGTAT CTGCAGTATG GGGACGACAC AAAGCAGATC 
CGCATGCCTA ATGAGATAAC AAGTGTTGAC ACAATCAGAG CCCTGTTCGT TAGTGCCTTC 
CCTCATCAGC TCAACATGAA GATGCTGGAG TCGCCCAGCA CTGCCATCTA CGTCAAAGAT 
GAGACCAGAA ATGTGTATTA TGAACTCACC GATGTCAGGA GCATCACAGA TCACTCCTGC 
CTGAAAGTGT ATCATAAAGA CCCAGCACAC GCCTTCAGTC ACGGACCCCG GCCGAGCAAT 
GGAGACACAA GGGTTCACAG GGAAATGATA TTTGCTGGAC GGGATGGTCA CCCACTCCGG 
AACCCCCCAA TGGGTCCTCC ACCCCCCCAC ATGCAAGGCT CCATGTCACC CACTACACCT 
CACGGGATGC CTCCCTCTCC ATCTCGAATT CCCTTTGGCC CGCGAAACCC AGGTGGAGGT 
GTTGCCACAA TGCCACGTGA GCGAGCCGGA CACACACTCC CAGCCCCTGC CCGAGCATCC 
TCCCCCTGCC CGAGTGCCAT CTTGGAGCGG CGGGATGTCA AACCTGACGA GGATGTAGCT 
GCGAAGACCA TGTCTCTGAA CACCAGAGCT GAAAGTCATT ACGCCGACCC TTATATGTTA 
CTTCACCATG CCCCGCCCCC TGACACAGTA GACCACGCCT ACCACCGTGC TACCATGCGC 
TCTTACAGCA ACCCCAGCGT TCCCTTGGAG CCCCCAGACC ATCTCTTCCG ACAGAAAAGC 
AGGAATAGCC AACTCCCCAT GCTTGGCTCC AAAACCCCGC CCCCTTCCCC GCACCGAATG 
GGTGAGATGA GAATGGTGGA CATCCATACG GTCCAACCGC AGAGCTCCAT GACTCTGGAA 
AGGGCTTCAC CTGTTAGACA ATCATTTCGG AAGGAGGCTC CTCCTATGGA TGCCATGGTA 
AAGGCAAGAG GGGGAATGGG GTCTCCAGCC ACCCCCGATC TTCAGGTTCA CAATCCTCTG 
GCGCCCCCGA CAGACCCTCA AACACGAGAG AGAATGAAGG CCATGGAGGA GCAAATAGCC 
AGTCTGACTG GTCTTGTGCA GCACGCTCTC TTAAAGAGTA ACAGCACTAA CAGCAACAAG 
GAGCACTCCA GCGAAAGACC AGTGAAGTCT GGATCACCAG CTCACAGCAC ATCTAGCACA 
GGAGGCTCTC CAGTATTAGC ACCAAAGGTT GTTGCTGCTC CTCCTGAGCG TAGCCCCGCC 
CCCACACCAC CTTTAGGACC TGCCCCTTTG CATGTTAACC TTCATCTGTT CAGGAAGAAT 
GTCTCTGAGC TCCGCCTCCA ACTACAACAT ATGAGACAAC TACAGCTTCA GAATCAGGAG 
TGTGTACGTG CTCAGATAAA GCGCGCAGAA CAGGAGATCG GTGTGAAACT GGCTGAAATG 
CTGAGACGAC AGGATGATCC GACACAGAAA CAGAGGATGC TTGTGGAGGA AGAGAGACAC 
CGATATCTCA GCATGCAGGA AAGAGTTCTC ACACAGCTGG GGGATCTGGA GCAGTTTGTG 
GAGACTCTGA GGACTGATTC GGGTCCTGCT GCGTCTCAGA GAGCAGTGAC GCTGAAGGAG 
GTTGAGGATG GGGCTGTCAG CCTGAGGAAG GTGGGAGAAA ACCTGGCTGG ACTCAAAGGT 
GAGTTTCCGG CTCTTCAGTC CCGCATGCGC TCAGTGCTCA GGGTTGAAGT AGAGGCTGTA 
AAGTTCCTCA AAGAAGAGCC ACACAAACTG GACAGCATGT TAAAGAGAGT CCGGACCCTC 
ACTGACACAC TCAGCAACCT TCGCAGGTGT GCCACCGATA ACCTGCTTAA ACCCACTGAT 
CAGGTTTGCT CAACACCTCC TGCCTCTGAA AAACCAGAAC TTATTGAATC CCCAGAACCT 
GCACCGGTCA CACCTCAGAG CTCCATCGTG AGGTCAGAGG TTATGACCTC AAACCCTGTG 
GTGATCCACC ATGCTCAGGC CTCTCCAGTA GCATCACAAC AATCCCAGCA ATCAACAGCA 
CCTTCACCCA TCCCTACACA AAGAAGAGAG TCTACACCGT CACCCACTAA ACAGCTCAGA 
AAACCCTCAC TGGAACAAAC ACAAAACAAC ACCAGTAATG GAGTTTCTGC TAATGGCGTA 
CATGGCAAGG GTCCTTCTCC AAACCTGATC ATTGAGGAGA TCCACGCCAG CCACAGCAAG 
AACAAGAACA GAGCTGTGTC CATAGAGGCT GCAGAGAAGG AATGGGAGGA GAAAAGGCAG 
AACTTGGGTC ATTATGATGG ACAGGAGTTT GAGCGGATTC TCCAGGAAGC AGAAGCAAAC 
ATGCTGAGGG GAATACCAAA CCTCGAGGTG CCCAGTCAAC TTGAGGGTTC TCCTGCTCCA 
CCTGCCATGC TTCCTGCCTC TCTGGAGGAG GTAGTAGAAA TAAACGAACC AGTAGCAGCA 
CCAAGAGAAC ACGATGACAA TCAGCCAAAC TCTGAGAAAC CAGCTAAGAC TGCTTCAGAG 
AAACCTCCCA AACCTCTGGA GAAACCAGTT AAGCCAGCCT TGGAGAAACC TCTTAAACCC 
AGCCTATCCA TCAAATCCAA CCTAGAGAGA CAGAGTAAGA CTCAGGAAAA AGTTAAACTT 
CAGAGCACAG AGAAAACCAA CAAGTCTCCT CCTCCACCCC CACCTCGACG CAACTACACC 
ACCAACACCG GCATGACCAC CACCCGCTCT GGAGAGGTCA TTTACACCAG CAGGAAAGAG 
TCTGTAACGG AAGGTGAAGA GGAGTCCAAC ATTTCAACAA TGTCTCAGCC TGAACCAAAG 
ACCCCCCCAG AGGTAAAGCC CAAACCCATC ACCCCTCCAC CTATCTCTGC CTCAGCCATC 
ACTGAAGAAG AGGACGAAGG AGACAAGATT ATGGCAGAGC TACAGGTTTT CCAGAAGTGC 
TCTGTTAAGG AGGTAGGGCT CAAGGGTTTG GTAGAGCCAC AGATCAGAGA GCTAAGACCT 
GGGGTCTTAC TGCCCCTTAA AGATAAGAAG AACACAGAGA ACCAAGATGA CAAGAATACT 
GACACAGATG AGAATGGCAA CAATACTATT CGTCAAAGTC CTGGGGTCAT CTACTATGTC 
ACAGCTCAGA TCTCCAAGGG AACTCCACCA CCGTCAGAGG AATCAACTGA GCGTAGAGAT 
GCATCGCAGT CTTTCCCATC ACAGGTGTCA CAAATCAATG CTTCTGACCA ATCCCAAAGC 
CAGCAGCAGG TTTCCAATAA CAAATCAGAT GCCCTCAAAA ACATTGGCCT AGCCAACTTT 
TCTGGCACCC AGCAACAAAA TCAGCTGTCT CGTTCAGCCT CACTAAAGTT ACATCCAGCC 
TCTTCATGGG AAGAAGTTCC TGGTAGCCCA ACACAAGGGA AGGTTCATGT GGTCAAGGCT 
GCTCAAGTGC AGGTTAGCAT AGAGGAGAGG GTAGAGTCTC CAACAGCTGT TTGCTCACCA 
GTCTCTTTTG GAGACTCTGA ACCCATCCTT TCTAAAGTGA TCCCTGTCGA GACAGCTTTG 
ACAAATCAAA AGCTTCCATT TGGTTCATCC AAGGTGAAGG AAAGGAAATT TGAGCAAATG 
GAGGAAAATA ATGAGGCTTT TCTCAGTCCC GATTTGTCAG GAGAAGAGCC ACCTCCTCCA 
CCACCTGATA ACTTTGCCTT TATGATAACC AACACCAAGG TTCAAGCCCT CTCCACTGGA 
GAGTACCATC AGCTTGTCAA CGCCAAGAAA GGAAGTGTAC AGACAGTGAC TTTTGGCAAC 
AAACAACCAA GTGCTTCTTA CAGTGGCATG GCATCAGGAG GTGATGCTAA TACCAATGGC 
CCTCCTGGAG CTGATAACAC TAGTAAAAAG CCAGTTATCA TAATCTTTGA TGAGCCAATG 
GACATCCGTT CAGCTTACAA GCGCCTCTCT ACCGTTTTCG AATGTGAGGA GGAGCTAGAT 
CGGATGCTTG CAGAAGAAAG AATAGATGAG GAGAGTGAGG AAACTGAGGA AGAAGAATGG 
ACGAGAGGAG AGAAGGTCAA ACAGAATGGA GTTGAAAGAC AGTCTGTAAA AAGAACTCTT 
GCAGATGTCA CAGATGGTGA AGTCAAGAAC TCTTCATCAT CCTTACTGGT TGCTGGTGAT 
GCAAAGCAAG ATGGGAAGAA AAAGTTTAAG TTGAAATTTC CCAAGAAACA GCTAGCAGCA 
TTAACACAAG CTATTCGGGC TGGAAGTAAA ACAGGCAAAA AGACTTTGCA AGTTGTCGTC 
TATGAGGATG AGGAAGAGTC CGATGGCACT GTCAAGCAAC ATAAAGAGAC CAAGAGGTAT 
GAAATTGCTA GCTCCAAAAC AGAATCCTCA AAATCAAATA CACAGGAATC TAATGGACGA 
ACTGATGAGA TTCGTAAGAG CACCTACAAA ACACTTGACA GTCTAGAGCA AACAATAAAG 
CAGCTAGAGA ATACAATTTG CGAAATGGGA CCAACGTCTT CGGAAGACAC TGAAAGCCTA 
ACGAATTCTG AAACTCAAGA AAACAAAGTA GTGGAGAATT CAGGCCACTC AGAGAAGGGA 
CTTTCTTCCA AAACCTTGGT TCCTAGGTTG TCTGCTGCCA GTAAGGGTCC TAGTCTCCGA 
AAGAAGACCA AACCACAGCT CCTTCCTCGG CCTACCAACA TACCAACTAC AGGGGTCTCA 
GTAACACCAG TCGCTACCCA CCAGAACGCC AACGTGGTCT CACCTACTAG TCGGATGCCA 
GTGCCAGTTT CTGTCAAGGC GCGGCAGCCA TCAAGCACCT CAGAAAAAGA GAGGGCAACA 
AAACCGCAAA AACTACAGGA CTCTCAGAGG CAGTTCCGCC AGGCTAATGG AAGTGCTAAG 
AGATCTGGAG GAGATCCCAA ATCCACTTCT CCTACCGTGC CTGCTTCTAA AATTCCCGCT 
TTTTCCACTA GTACGGGAAA AAGCTTGTCC CAGTCTGATT CTACTAATAA TGTTAATTCT 
TCTTCTCCTT TATCTTCTTC CCTTCCCACC AGCAAATCCT CTATCCCTCC TCCTCGATCC 
AGTTCCATCC CCTCCTCCCA CATCCCCTCT ATTTCTAACG GTTCTTTAAA ATATGCCCCT 
CCCACTCACA GCACTAAAGG CCTCTCTATT CCCACACAGA CACAAAATGG TCGACCTTCC 
ACTTCTTCTT CCTCCTCCTG TTCCCACTCC CCCTTGTCCC CGACTATGTT GAGTCAGAGT 
GCGAAGAGTA TCCGTACCAT TCACACACCC AGCTTCAACA GCTACAGTCG GCCGCACAAC 
GGAAGTGCCG GTAAATCAGC TATACCAACG GTAACTGTCG CTAAGGATGC TGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_005162591.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162648.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa28379
Clone ID Related Accession (Same CDS sequence)	XM_005162591.3 , XM_005162591.4
Accession Version	XM_005162591.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGGT GTCACAAATC 
AATGCTTCTG ACCAATCCCA AAGCCAGCAG CAGGTTTCCA ATAACAAATC AGATGCCCTC 
AAAAACATTG GCCTAGCCAA CTTTTCTGGC ACCCAGCAAC AAAATCAGCT GTCTCGTTCA 
GCCTCACTAA AGTTACATCC AGCCTCTTCA TGGGAAGAAG TTCCTGGTAG CCCAACACAA 
GGGAAGGTTC ATGTGGTCAA GGCTGCTCAA GTGCAGGTTA GCATAGAGGA GAGGGTAGAG 
TCTCCAACAG CTGTTTGCTC ACCAGTCTCT TTTGGAGACT CTGAACCCAT CCTTTCTAAA 
GTGATCCCTG TCGAGACAGC TTTGACAAAT CAAAAGCTTC CATTTGGTTC ATCCAAGGTG 
AAGGAAAGGA AATTTGAGCA AATGGAGGAA AATAATGAGG CTTTTCTCAG TCCCGATTTG 
TCAGGAGAAG AGCCACCTCC TCCACCACCT GATAACTTTG CCTTTATGAT AACCAACACC 
AAGGTTCAAG CCCTCTCCAC TGGAGAGTAC CATCAGCTTG TCAACGCCAA GAAAGGAAGT 
GTACAGACAG TGACTTTTGG CAACAAACAA CCAAGTGCTT CTTACAGTGG CATGGCATCA 
GGAGGTGATG CTAATACCAA TGGCCCTCCT GGAGCTGATA ACACTAGTAA AAAGCCAGTT 
ATCATAATCT TTGATGAGCC AATGGACATC CGTTCAGCTT ACAAGCGCCT CTCTACCGTT 
TTCGAATGTG AGGAGGAGCT AGATCGGATG CTTGCAGAAG AAAGAATAGA TGAGGAGAGT 
GAGGAAACTG AGGAAGAAGA ATGGACGAGA GGAGAGAAGG TCAAACAGAA TGGAGTTGAA 
AGACAGTCTG TAAAAAGAAC TCTTGCAGAT GTCACAGATG GTGAAGTCAA GAACTCTTCA 
TCATCCTTAC TGGTTGCTGG TGATGCAAAG CAAGATGGGA AGAAAAAGTT TAAGTTGAAA 
TTTCCCAAGA AACAGCTAGC AGCATTAACA CAAGCTATTC GGGCTGGAAG TAAAACAGGC 
AAAAAGACTT TGCAAGTTGT CGTCTATGAG GATGAGGAAG AGTCCGATGG CACTGTCAAG 
CAACATAAAG AGACCAAGAG GTATGAAATT GCTAGCTCCA AAACAGAATC CTCAAAATCA 
AATACACAGG AATCTAATGG ACGAACTGAT GAGATTCGTA AGAGCACCTA CAAAACACTT 
GACAGTCTAG AGCAAACAAT AAAGCAGCTA GAGAATACAA TTTGCGAAAT GGGACCAACG 
TCTTCGGAAG ACACTGAAAG CCTAACGAAT TCTGAAACTC AAGAAAACAA AGTAGTGGAG 
AATTCAGGCC ACTCAGAGAA GGGACTTTCT TCCAAAACCT TGGTTCCTAG GTTGTCTGCT 
GCCAGTAAGG GTCCTAGTCT CCGAAAGAAG ACCAAACCAC AGCTCCTTCC TCGGCCTACC 
AACATACCAA CTACAGGGGT CTCAGTAACA CCAGTCGCTA CCCACCAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGGCTA ATGGAAGTGC TAAGAGATCT GGAGGAGATC CCAAATCCAC TTCTCCTACC 
GTGCCTGCTT CTAAAATTCC CGCTTTTTCC ACTAGTACGG GAAAAAGCTT GTCCCAGTCT 
GATTCTACTA ATAATGTTAA TTCTTCTTCT CCTTTATCTT CTTCCCTTCC CACCAGCAAA 
TCCTCTATCC CTCCTCCTCG ATCCAGTTCC ATCCCCTCCT CCCACATCCC CTCTATTTCT 
AACGGTTCTT TAAAATATGC CCCTCCCACT CACAGCACTA AAGGCCTCTC TATTCCCACA 
CAGACACAAA ATGGTCGACC TTCCACTTCT TCTTCCTCCT CCTGTTCCCA CTCCCCCTTG 
TCCCCGACTA TGTTGAGTCA GAGTGCGAAG AGTATCCGTA CCATTCACAC ACCCAGCTTC 
AACAGCTACA GTCGGCCGCA CAACGGAAGT GCCGGTAAAT CAGCTATACC AACGGTAACT 
GTCGCTAAGG ATGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162648.1
CDS	367..5787
Translation	MSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_005162591.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162591.3

ORF Insert Sequence:

ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGGT GTCACAAATC 
AATGCTTCTG ACCAATCCCA AAGCCAGCAG CAGGTTTCCA ATAACAAATC AGATGCCCTC 
AAAAACATTG GCCTAGCCAA CTTTTCTGGC ACCCAGCAAC AAAATCAGCT GTCTCGTTCA 
GCCTCACTAA AGTTACATCC AGCCTCTTCA TGGGAAGAAG TTCCTGGTAG CCCAACACAA 
GGGAAGGTTC ATGTGGTCAA GGCTGCTCAA GTGCAGGTTA GCATAGAGGA GAGGGTAGAG 
TCTCCAACAG CTGTTTGCTC ACCAGTCTCT TTTGGAGACT CTGAACCCAT CCTTTCTAAA 
GTGATCCCTG TCGAGACAGC TTTGACAAAT CAAAAGCTTC CATTTGGTTC ATCCAAGGTG 
AAGGAAAGGA AATTTGAGCA AATGGAGGAA AATAATGAGG CTTTTCTCAG TCCCGATTTG 
TCAGGAGAAG AGCCACCTCC TCCACCACCT GATAACTTTG CCTTTATGAT AACCAACACC 
AAGGTTCAAG CCCTCTCCAC TGGAGAGTAC CATCAGCTTG TCAACGCCAA GAAAGGAAGT 
GTACAGACAG TGACTTTTGG CAACAAACAA CCAAGTGCTT CTTACAGTGG CATGGCATCA 
GGAGGTGATG CTAATACCAA TGGCCCTCCT GGAGCTGATA ACACTAGTAA AAAGCCAGTT 
ATCATAATCT TTGATGAGCC AATGGACATC CGTTCAGCTT ACAAGCGCCT CTCTACCGTT 
TTCGAATGTG AGGAGGAGCT AGATCGGATG CTTGCAGAAG AAAGAATAGA TGAGGAGAGT 
GAGGAAACTG AGGAAGAAGA ATGGACGAGA GGAGAGAAGG TCAAACAGAA TGGAGTTGAA 
AGACAGTCTG TAAAAAGAAC TCTTGCAGAT GTCACAGATG GTGAAGTCAA GAACTCTTCA 
TCATCCTTAC TGGTTGCTGG TGATGCAAAG CAAGATGGGA AGAAAAAGTT TAAGTTGAAA 
TTTCCCAAGA AACAGCTAGC AGCATTAACA CAAGCTATTC GGGCTGGAAG TAAAACAGGC 
AAAAAGACTT TGCAAGTTGT CGTCTATGAG GATGAGGAAG AGTCCGATGG CACTGTCAAG 
CAACATAAAG AGACCAAGAG GTATGAAATT GCTAGCTCCA AAACAGAATC CTCAAAATCA 
AATACACAGG AATCTAATGG ACGAACTGAT GAGATTCGTA AGAGCACCTA CAAAACACTT 
GACAGTCTAG AGCAAACAAT AAAGCAGCTA GAGAATACAA TTTGCGAAAT GGGACCAACG 
TCTTCGGAAG ACACTGAAAG CCTAACGAAT TCTGAAACTC AAGAAAACAA AGTAGTGGAG 
AATTCAGGCC ACTCAGAGAA GGGACTTTCT TCCAAAACCT TGGTTCCTAG GTTGTCTGCT 
GCCAGTAAGG GTCCTAGTCT CCGAAAGAAG ACCAAACCAC AGCTCCTTCC TCGGCCTACC 
AACATACCAA CTACAGGGGT CTCAGTAACA CCAGTCGCTA CCCACCAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGGCTA ATGGAAGTGC TAAGAGATCT GGAGGAGATC CCAAATCCAC TTCTCCTACC 
GTGCCTGCTT CTAAAATTCC CGCTTTTTCC ACTAGTACGG GAAAAAGCTT GTCCCAGTCT 
GATTCTACTA ATAATGTTAA TTCTTCTTCT CCTTTATCTT CTTCCCTTCC CACCAGCAAA 
TCCTCTATCC CTCCTCCTCG ATCCAGTTCC ATCCCCTCCT CCCACATCCC CTCTATTTCT 
AACGGTTCTT TAAAATATGC CCCTCCCACT CACAGCACTA AAGGCCTCTC TATTCCCACA 
CAGACACAAA ATGGTCGACC TTCCACTTCT TCTTCCTCCT CCTGTTCCCA CTCCCCCTTG 
TCCCCGACTA TGTTGAGTCA GAGTGCGAAG AGTATCCGTA CCATTCACAC ACCCAGCTTC 
AACAGCTACA GTCGGCCGCA CAACGGAAGT GCCGGTAAAT CAGCTATACC AACGGTAACT 
GTCGCTAAGG ATGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_005162591.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162648.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa28379
Clone ID Related Accession (Same CDS sequence)	XM_005162591.3 , XM_005162591.4
Accession Version	XM_005162591.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162591.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGGT GTCACAAATC 
AATGCTTCTG ACCAATCCCA AAGCCAGCAG CAGGTTTCCA ATAACAAATC AGATGCCCTC 
AAAAACATTG GCCTAGCCAA CTTTTCTGGC ACCCAGCAAC AAAATCAGCT GTCTCGTTCA 
GCCTCACTAA AGTTACATCC AGCCTCTTCA TGGGAAGAAG TTCCTGGTAG CCCAACACAA 
GGGAAGGTTC ATGTGGTCAA GGCTGCTCAA GTGCAGGTTA GCATAGAGGA GAGGGTAGAG 
TCTCCAACAG CTGTTTGCTC ACCAGTCTCT TTTGGAGACT CTGAACCCAT CCTTTCTAAA 
GTGATCCCTG TCGAGACAGC TTTGACAAAT CAAAAGCTTC CATTTGGTTC ATCCAAGGTG 
AAGGAAAGGA AATTTGAGCA AATGGAGGAA AATAATGAGG CTTTTCTCAG TCCCGATTTG 
TCAGGAGAAG AGCCACCTCC TCCACCACCT GATAACTTTG CCTTTATGAT AACCAACACC 
AAGGTTCAAG CCCTCTCCAC TGGAGAGTAC CATCAGCTTG TCAACGCCAA GAAAGGAAGT 
GTACAGACAG TGACTTTTGG CAACAAACAA CCAAGTGCTT CTTACAGTGG CATGGCATCA 
GGAGGTGATG CTAATACCAA TGGCCCTCCT GGAGCTGATA ACACTAGTAA AAAGCCAGTT 
ATCATAATCT TTGATGAGCC AATGGACATC CGTTCAGCTT ACAAGCGCCT CTCTACCGTT 
TTCGAATGTG AGGAGGAGCT AGATCGGATG CTTGCAGAAG AAAGAATAGA TGAGGAGAGT 
GAGGAAACTG AGGAAGAAGA ATGGACGAGA GGAGAGAAGG TCAAACAGAA TGGAGTTGAA 
AGACAGTCTG TAAAAAGAAC TCTTGCAGAT GTCACAGATG GTGAAGTCAA GAACTCTTCA 
TCATCCTTAC TGGTTGCTGG TGATGCAAAG CAAGATGGGA AGAAAAAGTT TAAGTTGAAA 
TTTCCCAAGA AACAGCTAGC AGCATTAACA CAAGCTATTC GGGCTGGAAG TAAAACAGGC 
AAAAAGACTT TGCAAGTTGT CGTCTATGAG GATGAGGAAG AGTCCGATGG CACTGTCAAG 
CAACATAAAG AGACCAAGAG GTATGAAATT GCTAGCTCCA AAACAGAATC CTCAAAATCA 
AATACACAGG AATCTAATGG ACGAACTGAT GAGATTCGTA AGAGCACCTA CAAAACACTT 
GACAGTCTAG AGCAAACAAT AAAGCAGCTA GAGAATACAA TTTGCGAAAT GGGACCAACG 
TCTTCGGAAG ACACTGAAAG CCTAACGAAT TCTGAAACTC AAGAAAACAA AGTAGTGGAG 
AATTCAGGCC ACTCAGAGAA GGGACTTTCT TCCAAAACCT TGGTTCCTAG GTTGTCTGCT 
GCCAGTAAGG GTCCTAGTCT CCGAAAGAAG ACCAAACCAC AGCTCCTTCC TCGGCCTACC 
AACATACCAA CTACAGGGGT CTCAGTAACA CCAGTCGCTA CCCACCAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGGCTA ATGGAAGTGC TAAGAGATCT GGAGGAGATC CCAAATCCAC TTCTCCTACC 
GTGCCTGCTT CTAAAATTCC CGCTTTTTCC ACTAGTACGG GAAAAAGCTT GTCCCAGTCT 
GATTCTACTA ATAATGTTAA TTCTTCTTCT CCTTTATCTT CTTCCCTTCC CACCAGCAAA 
TCCTCTATCC CTCCTCCTCG ATCCAGTTCC ATCCCCTCCT CCCACATCCC CTCTATTTCT 
AACGGTTCTT TAAAATATGC CCCTCCCACT CACAGCACTA AAGGCCTCTC TATTCCCACA 
CAGACACAAA ATGGTCGACC TTCCACTTCT TCTTCCTCCT CCTGTTCCCA CTCCCCCTTG 
TCCCCGACTA TGTTGAGTCA GAGTGCGAAG AGTATCCGTA CCATTCACAC ACCCAGCTTC 
AACAGCTACA GTCGGCCGCA CAACGGAAGT GCCGGTAAAT CAGCTATACC AACGGTAACT 
GTCGCTAAGG ATGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162648.1
CDS	324..5744
Translation	MSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_005162591.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162591.4

ORF Insert Sequence:

ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGGT GTCACAAATC 
AATGCTTCTG ACCAATCCCA AAGCCAGCAG CAGGTTTCCA ATAACAAATC AGATGCCCTC 
AAAAACATTG GCCTAGCCAA CTTTTCTGGC ACCCAGCAAC AAAATCAGCT GTCTCGTTCA 
GCCTCACTAA AGTTACATCC AGCCTCTTCA TGGGAAGAAG TTCCTGGTAG CCCAACACAA 
GGGAAGGTTC ATGTGGTCAA GGCTGCTCAA GTGCAGGTTA GCATAGAGGA GAGGGTAGAG 
TCTCCAACAG CTGTTTGCTC ACCAGTCTCT TTTGGAGACT CTGAACCCAT CCTTTCTAAA 
GTGATCCCTG TCGAGACAGC TTTGACAAAT CAAAAGCTTC CATTTGGTTC ATCCAAGGTG 
AAGGAAAGGA AATTTGAGCA AATGGAGGAA AATAATGAGG CTTTTCTCAG TCCCGATTTG 
TCAGGAGAAG AGCCACCTCC TCCACCACCT GATAACTTTG CCTTTATGAT AACCAACACC 
AAGGTTCAAG CCCTCTCCAC TGGAGAGTAC CATCAGCTTG TCAACGCCAA GAAAGGAAGT 
GTACAGACAG TGACTTTTGG CAACAAACAA CCAAGTGCTT CTTACAGTGG CATGGCATCA 
GGAGGTGATG CTAATACCAA TGGCCCTCCT GGAGCTGATA ACACTAGTAA AAAGCCAGTT 
ATCATAATCT TTGATGAGCC AATGGACATC CGTTCAGCTT ACAAGCGCCT CTCTACCGTT 
TTCGAATGTG AGGAGGAGCT AGATCGGATG CTTGCAGAAG AAAGAATAGA TGAGGAGAGT 
GAGGAAACTG AGGAAGAAGA ATGGACGAGA GGAGAGAAGG TCAAACAGAA TGGAGTTGAA 
AGACAGTCTG TAAAAAGAAC TCTTGCAGAT GTCACAGATG GTGAAGTCAA GAACTCTTCA 
TCATCCTTAC TGGTTGCTGG TGATGCAAAG CAAGATGGGA AGAAAAAGTT TAAGTTGAAA 
TTTCCCAAGA AACAGCTAGC AGCATTAACA CAAGCTATTC GGGCTGGAAG TAAAACAGGC 
AAAAAGACTT TGCAAGTTGT CGTCTATGAG GATGAGGAAG AGTCCGATGG CACTGTCAAG 
CAACATAAAG AGACCAAGAG GTATGAAATT GCTAGCTCCA AAACAGAATC CTCAAAATCA 
AATACACAGG AATCTAATGG ACGAACTGAT GAGATTCGTA AGAGCACCTA CAAAACACTT 
GACAGTCTAG AGCAAACAAT AAAGCAGCTA GAGAATACAA TTTGCGAAAT GGGACCAACG 
TCTTCGGAAG ACACTGAAAG CCTAACGAAT TCTGAAACTC AAGAAAACAA AGTAGTGGAG 
AATTCAGGCC ACTCAGAGAA GGGACTTTCT TCCAAAACCT TGGTTCCTAG GTTGTCTGCT 
GCCAGTAAGG GTCCTAGTCT CCGAAAGAAG ACCAAACCAC AGCTCCTTCC TCGGCCTACC 
AACATACCAA CTACAGGGGT CTCAGTAACA CCAGTCGCTA CCCACCAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGGCTA ATGGAAGTGC TAAGAGATCT GGAGGAGATC CCAAATCCAC TTCTCCTACC 
GTGCCTGCTT CTAAAATTCC CGCTTTTTCC ACTAGTACGG GAAAAAGCTT GTCCCAGTCT 
GATTCTACTA ATAATGTTAA TTCTTCTTCT CCTTTATCTT CTTCCCTTCC CACCAGCAAA 
TCCTCTATCC CTCCTCCTCG ATCCAGTTCC ATCCCCTCCT CCCACATCCC CTCTATTTCT 
AACGGTTCTT TAAAATATGC CCCTCCCACT CACAGCACTA AAGGCCTCTC TATTCCCACA 
CAGACACAAA ATGGTCGACC TTCCACTTCT TCTTCCTCCT CCTGTTCCCA CTCCCCCTTG 
TCCCCGACTA TGTTGAGTCA GAGTGCGAAG AGTATCCGTA CCATTCACAC ACCCAGCTTC 
AACAGCTACA GTCGGCCGCA CAACGGAAGT GCCGGTAAAT CAGCTATACC AACGGTAACT 
GTCGCTAAGG ATGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_685844.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_690936.6 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X11, mRNA.

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CloneID	ODa28380
Clone ID Related Accession (Same CDS sequence)	XM_685844.9 , XM_685844.8
Accession Version	XM_685844.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685844.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTTC GTGAGAAGGT AAACAAAGGA GTGCTGTATC TGCAGTATGG GGACGACACA 
AAGCAGATCC GCATGCCTAA TGAGATAACA AGTGTTGACA CAATCAGAGC CCTGTTCGTT 
AGTGCCTTCC CTCATCAGCT CAACATGAAG ATGCTGGAGT CGCCCAGCAC TGCCATCTAC 
GTCAAAGATG AGACCAGAAA TGTGTATTAT GAACTCACCG ATGTCAGGAG CATCACAGAT 
CACTCCTGCC TGAAAGTGTA TCATAAAGAC CCAGCACACG CCTTCAGTCA CGGACCCCGG 
CCGAGCAATG GAGACACAAG GGTTCACAGG GAAATGATAT TTGCTGGACG GGATGGTCAC 
CCACTCCGGA ACCCCCCAAT GGGTCCTCCA CCCCCCCACA TGCAAGGCTC CATGTCACCC 
ACTACACCTC ACGGGATGCC TCCCTCTCCA TCTCGAATTC CCTTTGGCCC GCGAAACCCA 
GGTGGAGGTG TTGCCACAAT GCCACGTGAG CGAGCCGGAC ACACACTCCC AGCCCCTGCC 
CGAGCATCCT CCCCCTGCCC GAGTGCCATC TTGGAGCGGC GGGATGTCAA ACCTGACGAG 
GATGTAGCTG CGAAGACCAT GTCTCTGAAC ACCAGAGCTG AAAGTCATTA CGCCGACCCT 
TATATGTTAC TTCACCATGC CCCGCCCCCT GACACAGTAG ACCACGCCTA CCACCGTGCT 
ACCATGCGCT CTTACAGCAA CCCCAGCGTT CCCTTGGAGC CCCCAGACCA TCTCTTCCGA 
CAGAAAAGCA GGAATAGCCA ACTCCCCATG CTTGGCTCCA AAACCCCGCC CCCTTCCCCG 
CACCGAATGG GTGAGATGAG AATGGTGGAC ATCCATACGG TCCAACCGCA GAGCTCCATG 
ACTCTGGAAA GGGCTTCACC TGTTAGACAA TCATTTCGGA AGGAGGCTCC TCCTATGGAT 
GCCATGGTAA AGGCAAGAGG GGGAATGGGG TCTCCAGCCA CCCCCGATCT TCAGGTTCAC 
AATCCTCTGG CGCCCCCGAC AGACCCTCAA ACACGAGAGA GAATGAAGGC CATGGAGGAG 
CAAATAGCCA GTCTGACTGG TCTTGTGCAG CACGCTCTCT TAAAGAGTAA CAGCACTAAC 
AGCAACAAGG AGCACTCCAG CGAAAGACCA GTGAAGTCTG GATCACCAGC TCACAGCACA 
TCTAGCACAG GAGGCTCTCC AGTATTAGCA CCAAAGGTTG TTGCTGCTCC TCCTGAGCGT 
AGCCCCGCCC CCACACCACC TTTAGGACCT GCCCCTTTGC ATGTTAACCT TCATCTGTTC 
AGGAAGAATG TCTCTGAGCT CCGCCTCCAA CTACAACATA TGAGACAACT ACAGCTTCAG 
AATCAGGAGT GTGTACGTGC TCAGATAAAG CGCGCAGAAC AGGAGATCGG TGTGAAACTG 
GCTGAAATGC TGAGACGACA GGATGATCCG ACACAGAAAC AGAGGATGCT TGTGGAGGAA 
GAGAGACACC GATATCTCAG CATGCAGGAA AGAGTTCTCA CACAGCTGGG GGATCTGGAG 
CAGTTTGTGG AGACTCTGAG GACTGATTCG GGTCCTGCTG CGTCTCAGAG AGCAGTGACG 
CTGAAGGAGG TTGAGGATGG GGCTGTCAGC CTGAGGAAGG TGGGAGAAAA CCTGGCTGGA 
CTCAAAGGTG AGTTTCCGGC TCTTCAGTCC CGCATGCGCT CAGTGCTCAG GGTTGAAGTA 
GAGGCTGTAA AGTTCCTCAA AGAAGAGCCA CACAAACTGG ACAGCATGTT AAAGAGAGTC 
CGGACCCTCA CTGACACACT CAGCAACCTT CGCAGGTGTG CCACCGATAA CCTGCTTAAA 
CCCACTGATC AGGTTTGCTC AACACCTCCT GCCTCTGAAA AACCAGAACT TATTGAATCC 
CCAGAACCTG CACCGGTCAC ACCTCAGAGC TCCATCGTGA GGTCAGAGGT TATGACCTCA 
AACCCTGTGG TGATCCACCA TGCTCAGGCC TCTCCAGTAG CATCACAACA ATCCCAGCAA 
TCAACAGCAC CTTCACCCAT CCCTACACAA AGAAGAGAGT CTACACCGTC ACCCACTAAA 
CAGCTCAGAA AACCCTCACT GGAACAAACA CAAAACAACA CCAGTAATGG AGTTTCTGCT 
AATGGCGTAC ATGGCAAGGG TCCTTCTCCA AACCTGATCA TTGAGGAGAT CCACGCCAGC 
CACAGCAAGA ACAAGAACAG AGCTGTGTCC ATAGAGGCTG CAGAGAAGGA ATGGGAGGAG 
AAAAGGCAGA ACTTGGGTCA TTATGATGGA CAGGAGTTTG AGCGGATTCT CCAGGAAGCA 
GAAGCAAACA TGCTGAGGGG AATACCAAAC CTCGAGGTGC CCAGTCAACT TGAGGGTTCT 
CCTGCTCCAC CTGCCATGCT TCCTGCCTCT CTGGAGGAGG TAGTAGAAAT AAACGAACCA 
GTAGCAGCAC CAAGAGAACA CGATGACAAT CAGCCAAACT CTGAGAAACC AGCTAAGACT 
GCTTCAGAGA AACCTCCCAA ACCTCTGGAG AAACCAGTTA AGCCAGCCTT GGAGAAACCT 
CTTAAACCCA GCCTATCCAT CAAATCCAAC CTAGAGAGAC AGAGTAAGAC TCAGGAAAAA 
GTTAAACTTC AGAGCACAGA GAAAACCAAC AAGTCTCCTC CTCCACCCCC ACCTCGACGC 
AACTACACCA CCAACACCGG CATGACCACC ACCCGCTCTG GAGAGGTCAT TTACACCAGC 
AGGAAAGAGT CTGTAACGGA AGGTGAAGAG GAGTCCAACA TTTCAACAAT GTCTCAGCCT 
GAACCAAAGA CCCCCCCAGA GGTAAAGCCC AAACCCATCA CCCCTCCACC TATCTCTGCC 
TCAGCCATCA CTGAAGAAGA GGACGAAGGA GACAAGATTA TGGCAGAGCT ACAGGTTTTC 
CAGAAGTGCT CTGTTAAGGA GGTAGGGCTC AAGGGTTTGG TAGAGCCACA GATCAGAGAG 
CTAAGACCTG GGGTCTTACT GCCCCTTAAA GATAAGAAGA ACACAGAGAA CCAAGATGAC 
AAGAATACTG ACACAGATGA GAATGGCAAC AATACTATTC GTCAAAGTCC TGGGGTCATC 
TACTATGTCA CAGCTCAGAT CTCCAAGGGA ACTCCACCAC CGTCAGAGGA ATCAACTGAG 
CGTAGAGATG CATCGCAGTC TTTCCCATCA CAGGTGTCAC AAATCAATGC TTCTGACCAA 
TCCCAAAGCC AGCAGCAGGT TTCCAATAAC AAATCAGATG CCCTCAAAAA CATTGGCCTA 
GCCAACTTTT CTGGCACCCA GCAACAAAAT CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA 
CATCCAGCCT CTTCATGGGA AGAAGTTCCT GGTAGCCCAA CACAAGGGAA GGTTCATGTG 
GTCAAGGCTG CTCAAGTGCA GGTTAGCATA GAGGAGAGGG TAGAGTCTCC AACAGCTGTT 
TGCTCACCAG TCTCTTTTGG AGACTCTGAA CCCATCCTTT CTAAAGTGAT CCCTGTCGAG 
ACAGCTTTGA CAAATCAAAA GCTTCCATTT GGTTCATCCA AGGTGAAGGA AAGGAAATTT 
GAGCAAATGG AGGAAAATAA TGAGGCTTTT CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA 
CCTCCTCCAC CACCTGATAA CTTTGCCTTT ATGATAACCA ACACCAAGGT TCAAGCCCTC 
TCCACTGGAG AGTACCATCA GCTTGTCAAC GCCAAGAAAG GAAGTGTACA GACAGTGACT 
TTTGGCAACA AACAACCAAG TGCTTCTTAC AGTGGCATGG CATCAGGAGG TGATGCTAAT 
ACCAATGGCC CTCCTGGAGC TGATAACACT AGTAAAAAGC CAGTTATCAT AATCTTTGAT 
GAGCCAATGG ACATCCGTTC AGCTTACAAG CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG 
GAGCTAGATC GGATGCTTGC AGAAGAAAGA ATAGATGAGG AGAGTGAGGA AACTGAGGAA 
GAAGAATGGA CGAGAGGAGA GAAGGTCAAA CAGAATGGAG TTGAAAGACA GTCTGTAAAA 
AGAACTCTTG CAGATGTCAC AGATGGTGAA GTCAAGAACT CTTCATCATC CTTACTGGTT 
GCTGGTGATG CAAAGCAAGA TGGGAAGAAA AAGTTTAAGT TGAAATTTCC CAAGAAACAG 
CTAGCAGCAT TAACACAAGC TATTCGGGCT GGAAGTAAAA CAGGCAAAAA GACTTTGCAA 
GTTGTCGTCT ATGAGGATGA GGAAGAGTCC GATGGCACTG TCAAGCAACA TAAAGAGACC 
AAGAGGTATG AAATTGCTAG CTCCAAAACA GAATCCTCAA AATCAAATAC ACAGGAATCT 
AATGGACGAA CTGATGAGAT TCGTAAGAGC ACCTACAAAA CACTTGACAG TCTAGAGCAA 
ACAATAAAGC AGCTAGAGAA TACAATTTGC GAAATGGGAC CAACGTCTTC GGAAGACACT 
GAAAGCCTAA CGAATTCTGA AACTCAAGAA AACAAAGTAG TGGAGAATTC AGGCCACTCA 
GAGAAGGGAC TTTCTTCCAA AACCTTGGTT CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT 
AGTCTCCGAA AGAAGACCAA ACCACAGCTC CTTCCTCGGC CTACCAACAT ACCAACTACA 
GGGGTCTCAG TAACACCAGT CGCTACCCAC CAGAACGCCA ACGTGGTCTC ACCTACTAGT 
CGGATGCCAG TGCCAGTTTC TGTCAAGGCG CGGCAGCCAT CAAGCACCTC AGAAAAAGAG 
AGGGCAACAA AACCGCAAAA ACTACAGGAC TCTCAGAGGC AGTTCCGCCA GGCTAATGGA 
AGTGCTAAGA GATCTGGAGG AGATCCCAAA TCCACTTCTC CTACCGTGCC TGCTTCTAAA 
ATTCCCGCTT TTTCCACTAG TACGGGAAAA AGCTTGTCCC AGTCTGATTC TACTAATAAT 
GTTAATTCTT CTTCTCCTTT ATCTTCTTCC CTTCCCACCA GCAAATCCTC TATCCCTCCT 
CCTCGATCCA GTTCCATCCC CTCCTCCCAC ATCCCCTCTA TTTCTAACGG TTCTTTAAAA 
TATGCCCCTC CCACTCACAG CACTAAAGGC CTCTCTATTC CCACACAGAC ACAAAATGGT 
CGACCTTCCA CTTCTTCTTC CTCCTCCTGT TCCCACTCCC CCTTGTCCCC GACTATGTTG 
AGTCAGAGTG CGAAGAGTAT CCGTACCATT CACACACCCA GCTTCAACAG CTACAGTCGG 
CCGCACAACG GAAGTGCCGG TAAATCAGCT ATACCAACGG TAACTGTCGC TAAGGATGCT 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690936.6
CDS	62..5407
Translation	MLFREKVNKGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_685844.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685844.9

ORF Insert Sequence:

ATGCTGTTTC GTGAGAAGGT AAACAAAGGA GTGCTGTATC TGCAGTATGG GGACGACACA 
AAGCAGATCC GCATGCCTAA TGAGATAACA AGTGTTGACA CAATCAGAGC CCTGTTCGTT 
AGTGCCTTCC CTCATCAGCT CAACATGAAG ATGCTGGAGT CGCCCAGCAC TGCCATCTAC 
GTCAAAGATG AGACCAGAAA TGTGTATTAT GAACTCACCG ATGTCAGGAG CATCACAGAT 
CACTCCTGCC TGAAAGTGTA TCATAAAGAC CCAGCACACG CCTTCAGTCA CGGACCCCGG 
CCGAGCAATG GAGACACAAG GGTTCACAGG GAAATGATAT TTGCTGGACG GGATGGTCAC 
CCACTCCGGA ACCCCCCAAT GGGTCCTCCA CCCCCCCACA TGCAAGGCTC CATGTCACCC 
ACTACACCTC ACGGGATGCC TCCCTCTCCA TCTCGAATTC CCTTTGGCCC GCGAAACCCA 
GGTGGAGGTG TTGCCACAAT GCCACGTGAG CGAGCCGGAC ACACACTCCC AGCCCCTGCC 
CGAGCATCCT CCCCCTGCCC GAGTGCCATC TTGGAGCGGC GGGATGTCAA ACCTGACGAG 
GATGTAGCTG CGAAGACCAT GTCTCTGAAC ACCAGAGCTG AAAGTCATTA CGCCGACCCT 
TATATGTTAC TTCACCATGC CCCGCCCCCT GACACAGTAG ACCACGCCTA CCACCGTGCT 
ACCATGCGCT CTTACAGCAA CCCCAGCGTT CCCTTGGAGC CCCCAGACCA TCTCTTCCGA 
CAGAAAAGCA GGAATAGCCA ACTCCCCATG CTTGGCTCCA AAACCCCGCC CCCTTCCCCG 
CACCGAATGG GTGAGATGAG AATGGTGGAC ATCCATACGG TCCAACCGCA GAGCTCCATG 
ACTCTGGAAA GGGCTTCACC TGTTAGACAA TCATTTCGGA AGGAGGCTCC TCCTATGGAT 
GCCATGGTAA AGGCAAGAGG GGGAATGGGG TCTCCAGCCA CCCCCGATCT TCAGGTTCAC 
AATCCTCTGG CGCCCCCGAC AGACCCTCAA ACACGAGAGA GAATGAAGGC CATGGAGGAG 
CAAATAGCCA GTCTGACTGG TCTTGTGCAG CACGCTCTCT TAAAGAGTAA CAGCACTAAC 
AGCAACAAGG AGCACTCCAG CGAAAGACCA GTGAAGTCTG GATCACCAGC TCACAGCACA 
TCTAGCACAG GAGGCTCTCC AGTATTAGCA CCAAAGGTTG TTGCTGCTCC TCCTGAGCGT 
AGCCCCGCCC CCACACCACC TTTAGGACCT GCCCCTTTGC ATGTTAACCT TCATCTGTTC 
AGGAAGAATG TCTCTGAGCT CCGCCTCCAA CTACAACATA TGAGACAACT ACAGCTTCAG 
AATCAGGAGT GTGTACGTGC TCAGATAAAG CGCGCAGAAC AGGAGATCGG TGTGAAACTG 
GCTGAAATGC TGAGACGACA GGATGATCCG ACACAGAAAC AGAGGATGCT TGTGGAGGAA 
GAGAGACACC GATATCTCAG CATGCAGGAA AGAGTTCTCA CACAGCTGGG GGATCTGGAG 
CAGTTTGTGG AGACTCTGAG GACTGATTCG GGTCCTGCTG CGTCTCAGAG AGCAGTGACG 
CTGAAGGAGG TTGAGGATGG GGCTGTCAGC CTGAGGAAGG TGGGAGAAAA CCTGGCTGGA 
CTCAAAGGTG AGTTTCCGGC TCTTCAGTCC CGCATGCGCT CAGTGCTCAG GGTTGAAGTA 
GAGGCTGTAA AGTTCCTCAA AGAAGAGCCA CACAAACTGG ACAGCATGTT AAAGAGAGTC 
CGGACCCTCA CTGACACACT CAGCAACCTT CGCAGGTGTG CCACCGATAA CCTGCTTAAA 
CCCACTGATC AGGTTTGCTC AACACCTCCT GCCTCTGAAA AACCAGAACT TATTGAATCC 
CCAGAACCTG CACCGGTCAC ACCTCAGAGC TCCATCGTGA GGTCAGAGGT TATGACCTCA 
AACCCTGTGG TGATCCACCA TGCTCAGGCC TCTCCAGTAG CATCACAACA ATCCCAGCAA 
TCAACAGCAC CTTCACCCAT CCCTACACAA AGAAGAGAGT CTACACCGTC ACCCACTAAA 
CAGCTCAGAA AACCCTCACT GGAACAAACA CAAAACAACA CCAGTAATGG AGTTTCTGCT 
AATGGCGTAC ATGGCAAGGG TCCTTCTCCA AACCTGATCA TTGAGGAGAT CCACGCCAGC 
CACAGCAAGA ACAAGAACAG AGCTGTGTCC ATAGAGGCTG CAGAGAAGGA ATGGGAGGAG 
AAAAGGCAGA ACTTGGGTCA TTATGATGGA CAGGAGTTTG AGCGGATTCT CCAGGAAGCA 
GAAGCAAACA TGCTGAGGGG AATACCAAAC CTCGAGGTGC CCAGTCAACT TGAGGGTTCT 
CCTGCTCCAC CTGCCATGCT TCCTGCCTCT CTGGAGGAGG TAGTAGAAAT AAACGAACCA 
GTAGCAGCAC CAAGAGAACA CGATGACAAT CAGCCAAACT CTGAGAAACC AGCTAAGACT 
GCTTCAGAGA AACCTCCCAA ACCTCTGGAG AAACCAGTTA AGCCAGCCTT GGAGAAACCT 
CTTAAACCCA GCCTATCCAT CAAATCCAAC CTAGAGAGAC AGAGTAAGAC TCAGGAAAAA 
GTTAAACTTC AGAGCACAGA GAAAACCAAC AAGTCTCCTC CTCCACCCCC ACCTCGACGC 
AACTACACCA CCAACACCGG CATGACCACC ACCCGCTCTG GAGAGGTCAT TTACACCAGC 
AGGAAAGAGT CTGTAACGGA AGGTGAAGAG GAGTCCAACA TTTCAACAAT GTCTCAGCCT 
GAACCAAAGA CCCCCCCAGA GGTAAAGCCC AAACCCATCA CCCCTCCACC TATCTCTGCC 
TCAGCCATCA CTGAAGAAGA GGACGAAGGA GACAAGATTA TGGCAGAGCT ACAGGTTTTC 
CAGAAGTGCT CTGTTAAGGA GGTAGGGCTC AAGGGTTTGG TAGAGCCACA GATCAGAGAG 
CTAAGACCTG GGGTCTTACT GCCCCTTAAA GATAAGAAGA ACACAGAGAA CCAAGATGAC 
AAGAATACTG ACACAGATGA GAATGGCAAC AATACTATTC GTCAAAGTCC TGGGGTCATC 
TACTATGTCA CAGCTCAGAT CTCCAAGGGA ACTCCACCAC CGTCAGAGGA ATCAACTGAG 
CGTAGAGATG CATCGCAGTC TTTCCCATCA CAGGTGTCAC AAATCAATGC TTCTGACCAA 
TCCCAAAGCC AGCAGCAGGT TTCCAATAAC AAATCAGATG CCCTCAAAAA CATTGGCCTA 
GCCAACTTTT CTGGCACCCA GCAACAAAAT CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA 
CATCCAGCCT CTTCATGGGA AGAAGTTCCT GGTAGCCCAA CACAAGGGAA GGTTCATGTG 
GTCAAGGCTG CTCAAGTGCA GGTTAGCATA GAGGAGAGGG TAGAGTCTCC AACAGCTGTT 
TGCTCACCAG TCTCTTTTGG AGACTCTGAA CCCATCCTTT CTAAAGTGAT CCCTGTCGAG 
ACAGCTTTGA CAAATCAAAA GCTTCCATTT GGTTCATCCA AGGTGAAGGA AAGGAAATTT 
GAGCAAATGG AGGAAAATAA TGAGGCTTTT CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA 
CCTCCTCCAC CACCTGATAA CTTTGCCTTT ATGATAACCA ACACCAAGGT TCAAGCCCTC 
TCCACTGGAG AGTACCATCA GCTTGTCAAC GCCAAGAAAG GAAGTGTACA GACAGTGACT 
TTTGGCAACA AACAACCAAG TGCTTCTTAC AGTGGCATGG CATCAGGAGG TGATGCTAAT 
ACCAATGGCC CTCCTGGAGC TGATAACACT AGTAAAAAGC CAGTTATCAT AATCTTTGAT 
GAGCCAATGG ACATCCGTTC AGCTTACAAG CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG 
GAGCTAGATC GGATGCTTGC AGAAGAAAGA ATAGATGAGG AGAGTGAGGA AACTGAGGAA 
GAAGAATGGA CGAGAGGAGA GAAGGTCAAA CAGAATGGAG TTGAAAGACA GTCTGTAAAA 
AGAACTCTTG CAGATGTCAC AGATGGTGAA GTCAAGAACT CTTCATCATC CTTACTGGTT 
GCTGGTGATG CAAAGCAAGA TGGGAAGAAA AAGTTTAAGT TGAAATTTCC CAAGAAACAG 
CTAGCAGCAT TAACACAAGC TATTCGGGCT GGAAGTAAAA CAGGCAAAAA GACTTTGCAA 
GTTGTCGTCT ATGAGGATGA GGAAGAGTCC GATGGCACTG TCAAGCAACA TAAAGAGACC 
AAGAGGTATG AAATTGCTAG CTCCAAAACA GAATCCTCAA AATCAAATAC ACAGGAATCT 
AATGGACGAA CTGATGAGAT TCGTAAGAGC ACCTACAAAA CACTTGACAG TCTAGAGCAA 
ACAATAAAGC AGCTAGAGAA TACAATTTGC GAAATGGGAC CAACGTCTTC GGAAGACACT 
GAAAGCCTAA CGAATTCTGA AACTCAAGAA AACAAAGTAG TGGAGAATTC AGGCCACTCA 
GAGAAGGGAC TTTCTTCCAA AACCTTGGTT CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT 
AGTCTCCGAA AGAAGACCAA ACCACAGCTC CTTCCTCGGC CTACCAACAT ACCAACTACA 
GGGGTCTCAG TAACACCAGT CGCTACCCAC CAGAACGCCA ACGTGGTCTC ACCTACTAGT 
CGGATGCCAG TGCCAGTTTC TGTCAAGGCG CGGCAGCCAT CAAGCACCTC AGAAAAAGAG 
AGGGCAACAA AACCGCAAAA ACTACAGGAC TCTCAGAGGC AGTTCCGCCA GGCTAATGGA 
AGTGCTAAGA GATCTGGAGG AGATCCCAAA TCCACTTCTC CTACCGTGCC TGCTTCTAAA 
ATTCCCGCTT TTTCCACTAG TACGGGAAAA AGCTTGTCCC AGTCTGATTC TACTAATAAT 
GTTAATTCTT CTTCTCCTTT ATCTTCTTCC CTTCCCACCA GCAAATCCTC TATCCCTCCT 
CCTCGATCCA GTTCCATCCC CTCCTCCCAC ATCCCCTCTA TTTCTAACGG TTCTTTAAAA 
TATGCCCCTC CCACTCACAG CACTAAAGGC CTCTCTATTC CCACACAGAC ACAAAATGGT 
CGACCTTCCA CTTCTTCTTC CTCCTCCTGT TCCCACTCCC CCTTGTCCCC GACTATGTTG 
AGTCAGAGTG CGAAGAGTAT CCGTACCATT CACACACCCA GCTTCAACAG CTACAGTCGG 
CCGCACAACG GAAGTGCCGG TAAATCAGCT ATACCAACGG TAACTGTCGC TAAGGATGCT 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_685844.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_690936.6 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X8, mRNA.

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CloneID	ODa28380
Clone ID Related Accession (Same CDS sequence)	XM_685844.9 , XM_685844.8
Accession Version	XM_685844.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685844.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTTTC GTGAGAAGGT AAACAAAGGA GTGCTGTATC TGCAGTATGG GGACGACACA 
AAGCAGATCC GCATGCCTAA TGAGATAACA AGTGTTGACA CAATCAGAGC CCTGTTCGTT 
AGTGCCTTCC CTCATCAGCT CAACATGAAG ATGCTGGAGT CGCCCAGCAC TGCCATCTAC 
GTCAAAGATG AGACCAGAAA TGTGTATTAT GAACTCACCG ATGTCAGGAG CATCACAGAT 
CACTCCTGCC TGAAAGTGTA TCATAAAGAC CCAGCACACG CCTTCAGTCA CGGACCCCGG 
CCGAGCAATG GAGACACAAG GGTTCACAGG GAAATGATAT TTGCTGGACG GGATGGTCAC 
CCACTCCGGA ACCCCCCAAT GGGTCCTCCA CCCCCCCACA TGCAAGGCTC CATGTCACCC 
ACTACACCTC ACGGGATGCC TCCCTCTCCA TCTCGAATTC CCTTTGGCCC GCGAAACCCA 
GGTGGAGGTG TTGCCACAAT GCCACGTGAG CGAGCCGGAC ACACACTCCC AGCCCCTGCC 
CGAGCATCCT CCCCCTGCCC GAGTGCCATC TTGGAGCGGC GGGATGTCAA ACCTGACGAG 
GATGTAGCTG CGAAGACCAT GTCTCTGAAC ACCAGAGCTG AAAGTCATTA CGCCGACCCT 
TATATGTTAC TTCACCATGC CCCGCCCCCT GACACAGTAG ACCACGCCTA CCACCGTGCT 
ACCATGCGCT CTTACAGCAA CCCCAGCGTT CCCTTGGAGC CCCCAGACCA TCTCTTCCGA 
CAGAAAAGCA GGAATAGCCA ACTCCCCATG CTTGGCTCCA AAACCCCGCC CCCTTCCCCG 
CACCGAATGG GTGAGATGAG AATGGTGGAC ATCCATACGG TCCAACCGCA GAGCTCCATG 
ACTCTGGAAA GGGCTTCACC TGTTAGACAA TCATTTCGGA AGGAGGCTCC TCCTATGGAT 
GCCATGGTAA AGGCAAGAGG GGGAATGGGG TCTCCAGCCA CCCCCGATCT TCAGGTTCAC 
AATCCTCTGG CGCCCCCGAC AGACCCTCAA ACACGAGAGA GAATGAAGGC CATGGAGGAG 
CAAATAGCCA GTCTGACTGG TCTTGTGCAG CACGCTCTCT TAAAGAGTAA CAGCACTAAC 
AGCAACAAGG AGCACTCCAG CGAAAGACCA GTGAAGTCTG GATCACCAGC TCACAGCACA 
TCTAGCACAG GAGGCTCTCC AGTATTAGCA CCAAAGGTTG TTGCTGCTCC TCCTGAGCGT 
AGCCCCGCCC CCACACCACC TTTAGGACCT GCCCCTTTGC ATGTTAACCT TCATCTGTTC 
AGGAAGAATG TCTCTGAGCT CCGCCTCCAA CTACAACATA TGAGACAACT ACAGCTTCAG 
AATCAGGAGT GTGTACGTGC TCAGATAAAG CGCGCAGAAC AGGAGATCGG TGTGAAACTG 
GCTGAAATGC TGAGACGACA GGATGATCCG ACACAGAAAC AGAGGATGCT TGTGGAGGAA 
GAGAGACACC GATATCTCAG CATGCAGGAA AGAGTTCTCA CACAGCTGGG GGATCTGGAG 
CAGTTTGTGG AGACTCTGAG GACTGATTCG GGTCCTGCTG CGTCTCAGAG AGCAGTGACG 
CTGAAGGAGG TTGAGGATGG GGCTGTCAGC CTGAGGAAGG TGGGAGAAAA CCTGGCTGGA 
CTCAAAGGTG AGTTTCCGGC TCTTCAGTCC CGCATGCGCT CAGTGCTCAG GGTTGAAGTA 
GAGGCTGTAA AGTTCCTCAA AGAAGAGCCA CACAAACTGG ACAGCATGTT AAAGAGAGTC 
CGGACCCTCA CTGACACACT CAGCAACCTT CGCAGGTGTG CCACCGATAA CCTGCTTAAA 
CCCACTGATC AGGTTTGCTC AACACCTCCT GCCTCTGAAA AACCAGAACT TATTGAATCC 
CCAGAACCTG CACCGGTCAC ACCTCAGAGC TCCATCGTGA GGTCAGAGGT TATGACCTCA 
AACCCTGTGG TGATCCACCA TGCTCAGGCC TCTCCAGTAG CATCACAACA ATCCCAGCAA 
TCAACAGCAC CTTCACCCAT CCCTACACAA AGAAGAGAGT CTACACCGTC ACCCACTAAA 
CAGCTCAGAA AACCCTCACT GGAACAAACA CAAAACAACA CCAGTAATGG AGTTTCTGCT 
AATGGCGTAC ATGGCAAGGG TCCTTCTCCA AACCTGATCA TTGAGGAGAT CCACGCCAGC 
CACAGCAAGA ACAAGAACAG AGCTGTGTCC ATAGAGGCTG CAGAGAAGGA ATGGGAGGAG 
AAAAGGCAGA ACTTGGGTCA TTATGATGGA CAGGAGTTTG AGCGGATTCT CCAGGAAGCA 
GAAGCAAACA TGCTGAGGGG AATACCAAAC CTCGAGGTGC CCAGTCAACT TGAGGGTTCT 
CCTGCTCCAC CTGCCATGCT TCCTGCCTCT CTGGAGGAGG TAGTAGAAAT AAACGAACCA 
GTAGCAGCAC CAAGAGAACA CGATGACAAT CAGCCAAACT CTGAGAAACC AGCTAAGACT 
GCTTCAGAGA AACCTCCCAA ACCTCTGGAG AAACCAGTTA AGCCAGCCTT GGAGAAACCT 
CTTAAACCCA GCCTATCCAT CAAATCCAAC CTAGAGAGAC AGAGTAAGAC TCAGGAAAAA 
GTTAAACTTC AGAGCACAGA GAAAACCAAC AAGTCTCCTC CTCCACCCCC ACCTCGACGC 
AACTACACCA CCAACACCGG CATGACCACC ACCCGCTCTG GAGAGGTCAT TTACACCAGC 
AGGAAAGAGT CTGTAACGGA AGGTGAAGAG GAGTCCAACA TTTCAACAAT GTCTCAGCCT 
GAACCAAAGA CCCCCCCAGA GGTAAAGCCC AAACCCATCA CCCCTCCACC TATCTCTGCC 
TCAGCCATCA CTGAAGAAGA GGACGAAGGA GACAAGATTA TGGCAGAGCT ACAGGTTTTC 
CAGAAGTGCT CTGTTAAGGA GGTAGGGCTC AAGGGTTTGG TAGAGCCACA GATCAGAGAG 
CTAAGACCTG GGGTCTTACT GCCCCTTAAA GATAAGAAGA ACACAGAGAA CCAAGATGAC 
AAGAATACTG ACACAGATGA GAATGGCAAC AATACTATTC GTCAAAGTCC TGGGGTCATC 
TACTATGTCA CAGCTCAGAT CTCCAAGGGA ACTCCACCAC CGTCAGAGGA ATCAACTGAG 
CGTAGAGATG CATCGCAGTC TTTCCCATCA CAGGTGTCAC AAATCAATGC TTCTGACCAA 
TCCCAAAGCC AGCAGCAGGT TTCCAATAAC AAATCAGATG CCCTCAAAAA CATTGGCCTA 
GCCAACTTTT CTGGCACCCA GCAACAAAAT CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA 
CATCCAGCCT CTTCATGGGA AGAAGTTCCT GGTAGCCCAA CACAAGGGAA GGTTCATGTG 
GTCAAGGCTG CTCAAGTGCA GGTTAGCATA GAGGAGAGGG TAGAGTCTCC AACAGCTGTT 
TGCTCACCAG TCTCTTTTGG AGACTCTGAA CCCATCCTTT CTAAAGTGAT CCCTGTCGAG 
ACAGCTTTGA CAAATCAAAA GCTTCCATTT GGTTCATCCA AGGTGAAGGA AAGGAAATTT 
GAGCAAATGG AGGAAAATAA TGAGGCTTTT CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA 
CCTCCTCCAC CACCTGATAA CTTTGCCTTT ATGATAACCA ACACCAAGGT TCAAGCCCTC 
TCCACTGGAG AGTACCATCA GCTTGTCAAC GCCAAGAAAG GAAGTGTACA GACAGTGACT 
TTTGGCAACA AACAACCAAG TGCTTCTTAC AGTGGCATGG CATCAGGAGG TGATGCTAAT 
ACCAATGGCC CTCCTGGAGC TGATAACACT AGTAAAAAGC CAGTTATCAT AATCTTTGAT 
GAGCCAATGG ACATCCGTTC AGCTTACAAG CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG 
GAGCTAGATC GGATGCTTGC AGAAGAAAGA ATAGATGAGG AGAGTGAGGA AACTGAGGAA 
GAAGAATGGA CGAGAGGAGA GAAGGTCAAA CAGAATGGAG TTGAAAGACA GTCTGTAAAA 
AGAACTCTTG CAGATGTCAC AGATGGTGAA GTCAAGAACT CTTCATCATC CTTACTGGTT 
GCTGGTGATG CAAAGCAAGA TGGGAAGAAA AAGTTTAAGT TGAAATTTCC CAAGAAACAG 
CTAGCAGCAT TAACACAAGC TATTCGGGCT GGAAGTAAAA CAGGCAAAAA GACTTTGCAA 
GTTGTCGTCT ATGAGGATGA GGAAGAGTCC GATGGCACTG TCAAGCAACA TAAAGAGACC 
AAGAGGTATG AAATTGCTAG CTCCAAAACA GAATCCTCAA AATCAAATAC ACAGGAATCT 
AATGGACGAA CTGATGAGAT TCGTAAGAGC ACCTACAAAA CACTTGACAG TCTAGAGCAA 
ACAATAAAGC AGCTAGAGAA TACAATTTGC GAAATGGGAC CAACGTCTTC GGAAGACACT 
GAAAGCCTAA CGAATTCTGA AACTCAAGAA AACAAAGTAG TGGAGAATTC AGGCCACTCA 
GAGAAGGGAC TTTCTTCCAA AACCTTGGTT CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT 
AGTCTCCGAA AGAAGACCAA ACCACAGCTC CTTCCTCGGC CTACCAACAT ACCAACTACA 
GGGGTCTCAG TAACACCAGT CGCTACCCAC CAGAACGCCA ACGTGGTCTC ACCTACTAGT 
CGGATGCCAG TGCCAGTTTC TGTCAAGGCG CGGCAGCCAT CAAGCACCTC AGAAAAAGAG 
AGGGCAACAA AACCGCAAAA ACTACAGGAC TCTCAGAGGC AGTTCCGCCA GGCTAATGGA 
AGTGCTAAGA GATCTGGAGG AGATCCCAAA TCCACTTCTC CTACCGTGCC TGCTTCTAAA 
ATTCCCGCTT TTTCCACTAG TACGGGAAAA AGCTTGTCCC AGTCTGATTC TACTAATAAT 
GTTAATTCTT CTTCTCCTTT ATCTTCTTCC CTTCCCACCA GCAAATCCTC TATCCCTCCT 
CCTCGATCCA GTTCCATCCC CTCCTCCCAC ATCCCCTCTA TTTCTAACGG TTCTTTAAAA 
TATGCCCCTC CCACTCACAG CACTAAAGGC CTCTCTATTC CCACACAGAC ACAAAATGGT 
CGACCTTCCA CTTCTTCTTC CTCCTCCTGT TCCCACTCCC CCTTGTCCCC GACTATGTTG 
AGTCAGAGTG CGAAGAGTAT CCGTACCATT CACACACCCA GCTTCAACAG CTACAGTCGG 
CCGCACAACG GAAGTGCCGG TAAATCAGCT ATACCAACGG TAACTGTCGC TAAGGATGCT 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690936.6
CDS	53..5398
Translation	MLFREKVNKGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_685844.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685844.8

ORF Insert Sequence:

ATGCTGTTTC GTGAGAAGGT AAACAAAGGA GTGCTGTATC TGCAGTATGG GGACGACACA 
AAGCAGATCC GCATGCCTAA TGAGATAACA AGTGTTGACA CAATCAGAGC CCTGTTCGTT 
AGTGCCTTCC CTCATCAGCT CAACATGAAG ATGCTGGAGT CGCCCAGCAC TGCCATCTAC 
GTCAAAGATG AGACCAGAAA TGTGTATTAT GAACTCACCG ATGTCAGGAG CATCACAGAT 
CACTCCTGCC TGAAAGTGTA TCATAAAGAC CCAGCACACG CCTTCAGTCA CGGACCCCGG 
CCGAGCAATG GAGACACAAG GGTTCACAGG GAAATGATAT TTGCTGGACG GGATGGTCAC 
CCACTCCGGA ACCCCCCAAT GGGTCCTCCA CCCCCCCACA TGCAAGGCTC CATGTCACCC 
ACTACACCTC ACGGGATGCC TCCCTCTCCA TCTCGAATTC CCTTTGGCCC GCGAAACCCA 
GGTGGAGGTG TTGCCACAAT GCCACGTGAG CGAGCCGGAC ACACACTCCC AGCCCCTGCC 
CGAGCATCCT CCCCCTGCCC GAGTGCCATC TTGGAGCGGC GGGATGTCAA ACCTGACGAG 
GATGTAGCTG CGAAGACCAT GTCTCTGAAC ACCAGAGCTG AAAGTCATTA CGCCGACCCT 
TATATGTTAC TTCACCATGC CCCGCCCCCT GACACAGTAG ACCACGCCTA CCACCGTGCT 
ACCATGCGCT CTTACAGCAA CCCCAGCGTT CCCTTGGAGC CCCCAGACCA TCTCTTCCGA 
CAGAAAAGCA GGAATAGCCA ACTCCCCATG CTTGGCTCCA AAACCCCGCC CCCTTCCCCG 
CACCGAATGG GTGAGATGAG AATGGTGGAC ATCCATACGG TCCAACCGCA GAGCTCCATG 
ACTCTGGAAA GGGCTTCACC TGTTAGACAA TCATTTCGGA AGGAGGCTCC TCCTATGGAT 
GCCATGGTAA AGGCAAGAGG GGGAATGGGG TCTCCAGCCA CCCCCGATCT TCAGGTTCAC 
AATCCTCTGG CGCCCCCGAC AGACCCTCAA ACACGAGAGA GAATGAAGGC CATGGAGGAG 
CAAATAGCCA GTCTGACTGG TCTTGTGCAG CACGCTCTCT TAAAGAGTAA CAGCACTAAC 
AGCAACAAGG AGCACTCCAG CGAAAGACCA GTGAAGTCTG GATCACCAGC TCACAGCACA 
TCTAGCACAG GAGGCTCTCC AGTATTAGCA CCAAAGGTTG TTGCTGCTCC TCCTGAGCGT 
AGCCCCGCCC CCACACCACC TTTAGGACCT GCCCCTTTGC ATGTTAACCT TCATCTGTTC 
AGGAAGAATG TCTCTGAGCT CCGCCTCCAA CTACAACATA TGAGACAACT ACAGCTTCAG 
AATCAGGAGT GTGTACGTGC TCAGATAAAG CGCGCAGAAC AGGAGATCGG TGTGAAACTG 
GCTGAAATGC TGAGACGACA GGATGATCCG ACACAGAAAC AGAGGATGCT TGTGGAGGAA 
GAGAGACACC GATATCTCAG CATGCAGGAA AGAGTTCTCA CACAGCTGGG GGATCTGGAG 
CAGTTTGTGG AGACTCTGAG GACTGATTCG GGTCCTGCTG CGTCTCAGAG AGCAGTGACG 
CTGAAGGAGG TTGAGGATGG GGCTGTCAGC CTGAGGAAGG TGGGAGAAAA CCTGGCTGGA 
CTCAAAGGTG AGTTTCCGGC TCTTCAGTCC CGCATGCGCT CAGTGCTCAG GGTTGAAGTA 
GAGGCTGTAA AGTTCCTCAA AGAAGAGCCA CACAAACTGG ACAGCATGTT AAAGAGAGTC 
CGGACCCTCA CTGACACACT CAGCAACCTT CGCAGGTGTG CCACCGATAA CCTGCTTAAA 
CCCACTGATC AGGTTTGCTC AACACCTCCT GCCTCTGAAA AACCAGAACT TATTGAATCC 
CCAGAACCTG CACCGGTCAC ACCTCAGAGC TCCATCGTGA GGTCAGAGGT TATGACCTCA 
AACCCTGTGG TGATCCACCA TGCTCAGGCC TCTCCAGTAG CATCACAACA ATCCCAGCAA 
TCAACAGCAC CTTCACCCAT CCCTACACAA AGAAGAGAGT CTACACCGTC ACCCACTAAA 
CAGCTCAGAA AACCCTCACT GGAACAAACA CAAAACAACA CCAGTAATGG AGTTTCTGCT 
AATGGCGTAC ATGGCAAGGG TCCTTCTCCA AACCTGATCA TTGAGGAGAT CCACGCCAGC 
CACAGCAAGA ACAAGAACAG AGCTGTGTCC ATAGAGGCTG CAGAGAAGGA ATGGGAGGAG 
AAAAGGCAGA ACTTGGGTCA TTATGATGGA CAGGAGTTTG AGCGGATTCT CCAGGAAGCA 
GAAGCAAACA TGCTGAGGGG AATACCAAAC CTCGAGGTGC CCAGTCAACT TGAGGGTTCT 
CCTGCTCCAC CTGCCATGCT TCCTGCCTCT CTGGAGGAGG TAGTAGAAAT AAACGAACCA 
GTAGCAGCAC CAAGAGAACA CGATGACAAT CAGCCAAACT CTGAGAAACC AGCTAAGACT 
GCTTCAGAGA AACCTCCCAA ACCTCTGGAG AAACCAGTTA AGCCAGCCTT GGAGAAACCT 
CTTAAACCCA GCCTATCCAT CAAATCCAAC CTAGAGAGAC AGAGTAAGAC TCAGGAAAAA 
GTTAAACTTC AGAGCACAGA GAAAACCAAC AAGTCTCCTC CTCCACCCCC ACCTCGACGC 
AACTACACCA CCAACACCGG CATGACCACC ACCCGCTCTG GAGAGGTCAT TTACACCAGC 
AGGAAAGAGT CTGTAACGGA AGGTGAAGAG GAGTCCAACA TTTCAACAAT GTCTCAGCCT 
GAACCAAAGA CCCCCCCAGA GGTAAAGCCC AAACCCATCA CCCCTCCACC TATCTCTGCC 
TCAGCCATCA CTGAAGAAGA GGACGAAGGA GACAAGATTA TGGCAGAGCT ACAGGTTTTC 
CAGAAGTGCT CTGTTAAGGA GGTAGGGCTC AAGGGTTTGG TAGAGCCACA GATCAGAGAG 
CTAAGACCTG GGGTCTTACT GCCCCTTAAA GATAAGAAGA ACACAGAGAA CCAAGATGAC 
AAGAATACTG ACACAGATGA GAATGGCAAC AATACTATTC GTCAAAGTCC TGGGGTCATC 
TACTATGTCA CAGCTCAGAT CTCCAAGGGA ACTCCACCAC CGTCAGAGGA ATCAACTGAG 
CGTAGAGATG CATCGCAGTC TTTCCCATCA CAGGTGTCAC AAATCAATGC TTCTGACCAA 
TCCCAAAGCC AGCAGCAGGT TTCCAATAAC AAATCAGATG CCCTCAAAAA CATTGGCCTA 
GCCAACTTTT CTGGCACCCA GCAACAAAAT CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA 
CATCCAGCCT CTTCATGGGA AGAAGTTCCT GGTAGCCCAA CACAAGGGAA GGTTCATGTG 
GTCAAGGCTG CTCAAGTGCA GGTTAGCATA GAGGAGAGGG TAGAGTCTCC AACAGCTGTT 
TGCTCACCAG TCTCTTTTGG AGACTCTGAA CCCATCCTTT CTAAAGTGAT CCCTGTCGAG 
ACAGCTTTGA CAAATCAAAA GCTTCCATTT GGTTCATCCA AGGTGAAGGA AAGGAAATTT 
GAGCAAATGG AGGAAAATAA TGAGGCTTTT CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA 
CCTCCTCCAC CACCTGATAA CTTTGCCTTT ATGATAACCA ACACCAAGGT TCAAGCCCTC 
TCCACTGGAG AGTACCATCA GCTTGTCAAC GCCAAGAAAG GAAGTGTACA GACAGTGACT 
TTTGGCAACA AACAACCAAG TGCTTCTTAC AGTGGCATGG CATCAGGAGG TGATGCTAAT 
ACCAATGGCC CTCCTGGAGC TGATAACACT AGTAAAAAGC CAGTTATCAT AATCTTTGAT 
GAGCCAATGG ACATCCGTTC AGCTTACAAG CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG 
GAGCTAGATC GGATGCTTGC AGAAGAAAGA ATAGATGAGG AGAGTGAGGA AACTGAGGAA 
GAAGAATGGA CGAGAGGAGA GAAGGTCAAA CAGAATGGAG TTGAAAGACA GTCTGTAAAA 
AGAACTCTTG CAGATGTCAC AGATGGTGAA GTCAAGAACT CTTCATCATC CTTACTGGTT 
GCTGGTGATG CAAAGCAAGA TGGGAAGAAA AAGTTTAAGT TGAAATTTCC CAAGAAACAG 
CTAGCAGCAT TAACACAAGC TATTCGGGCT GGAAGTAAAA CAGGCAAAAA GACTTTGCAA 
GTTGTCGTCT ATGAGGATGA GGAAGAGTCC GATGGCACTG TCAAGCAACA TAAAGAGACC 
AAGAGGTATG AAATTGCTAG CTCCAAAACA GAATCCTCAA AATCAAATAC ACAGGAATCT 
AATGGACGAA CTGATGAGAT TCGTAAGAGC ACCTACAAAA CACTTGACAG TCTAGAGCAA 
ACAATAAAGC AGCTAGAGAA TACAATTTGC GAAATGGGAC CAACGTCTTC GGAAGACACT 
GAAAGCCTAA CGAATTCTGA AACTCAAGAA AACAAAGTAG TGGAGAATTC AGGCCACTCA 
GAGAAGGGAC TTTCTTCCAA AACCTTGGTT CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT 
AGTCTCCGAA AGAAGACCAA ACCACAGCTC CTTCCTCGGC CTACCAACAT ACCAACTACA 
GGGGTCTCAG TAACACCAGT CGCTACCCAC CAGAACGCCA ACGTGGTCTC ACCTACTAGT 
CGGATGCCAG TGCCAGTTTC TGTCAAGGCG CGGCAGCCAT CAAGCACCTC AGAAAAAGAG 
AGGGCAACAA AACCGCAAAA ACTACAGGAC TCTCAGAGGC AGTTCCGCCA GGCTAATGGA 
AGTGCTAAGA GATCTGGAGG AGATCCCAAA TCCACTTCTC CTACCGTGCC TGCTTCTAAA 
ATTCCCGCTT TTTCCACTAG TACGGGAAAA AGCTTGTCCC AGTCTGATTC TACTAATAAT 
GTTAATTCTT CTTCTCCTTT ATCTTCTTCC CTTCCCACCA GCAAATCCTC TATCCCTCCT 
CCTCGATCCA GTTCCATCCC CTCCTCCCAC ATCCCCTCTA TTTCTAACGG TTCTTTAAAA 
TATGCCCCTC CCACTCACAG CACTAAAGGC CTCTCTATTC CCACACAGAC ACAAAATGGT 
CGACCTTCCA CTTCTTCTTC CTCCTCCTGT TCCCACTCCC CCTTGTCCCC GACTATGTTG 
AGTCAGAGTG CGAAGAGTAT CCGTACCATT CACACACCCA GCTTCAACAG CTACAGTCGG 
CCGCACAACG GAAGTGCCGG TAAATCAGCT ATACCAACGG TAACTGTCGC TAAGGATGCT 
GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_005162592.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162649.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X9, mRNA.

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CloneID	ODa28381
Clone ID Related Accession (Same CDS sequence)	XM_005162592.3
Accession Version	XM_005162592.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4614bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162592.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC ACAGAGAACC AAGATGACAA GAATACTGAC 
ACAGATGAGA ATGGCAACAA TACTATTCGT CAAAGTCCTG GGGTCATCTA CTATGTCACA 
GCTCAGATCT CCAAGGGAAC TCCACCACCG TCAGAGGAAT CAACTGAGCG TAGAGATGCA 
TCGCAGTCTT TCCCATCACA GAACGCCAAC GTGGTCTCAC CTACTAGTCG GATGCCAGTG 
CCAGTTTCTG TCAAGGCGCG GCAGCCATCA AGCACCTCAG AAAAAGAGAG GGCAACAAAA 
CCGCAAAAAC TACAGGACTC TCAGAGGCAG TTCCGCCAGG CTAATGGAAG TGCTAAGAGA 
TCTGGAGGAG ATCCCAAATC CACTTCTCCT ACCGTGCCTG CTTCTAAAAT TCCCGCTTTT 
TCCACTAGTA CGGGAAAAAG CTTGTCCCAG TCTGATTCTA CTAATAATGT TAATTCTTCT 
TCTCCTTTAT CTTCTTCCCT TCCCACCAGC AAATCCTCTA TCCCTCCTCC TCGATCCAGT 
TCCATCCCCT CCTCCCACAT CCCCTCTATT TCTAACGGTT CTTTAAAATA TGCCCCTCCC 
ACTCACAGCA CTAAAGGCCT CTCTATTCCC ACACAGACAC AAAATGGTCG ACCTTCCACT 
TCTTCTTCCT CCTCCTGTTC CCACTCCCCC TTGTCCCCGA CTATGTTGAG TCAGAGTGCG 
AAGAGTATCC GTACCATTCA CACACCCAGC TTCAACAGCT ACAGTCGGCC GCACAACGGA 
AGTGCCGGTA AATCAGCTAT ACCAACGGTA ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162649.1
CDS	613..5226
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_005162592.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162592.3

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC ACAGAGAACC AAGATGACAA GAATACTGAC 
ACAGATGAGA ATGGCAACAA TACTATTCGT CAAAGTCCTG GGGTCATCTA CTATGTCACA 
GCTCAGATCT CCAAGGGAAC TCCACCACCG TCAGAGGAAT CAACTGAGCG TAGAGATGCA 
TCGCAGTCTT TCCCATCACA GAACGCCAAC GTGGTCTCAC CTACTAGTCG GATGCCAGTG 
CCAGTTTCTG TCAAGGCGCG GCAGCCATCA AGCACCTCAG AAAAAGAGAG GGCAACAAAA 
CCGCAAAAAC TACAGGACTC TCAGAGGCAG TTCCGCCAGG CTAATGGAAG TGCTAAGAGA 
TCTGGAGGAG ATCCCAAATC CACTTCTCCT ACCGTGCCTG CTTCTAAAAT TCCCGCTTTT 
TCCACTAGTA CGGGAAAAAG CTTGTCCCAG TCTGATTCTA CTAATAATGT TAATTCTTCT 
TCTCCTTTAT CTTCTTCCCT TCCCACCAGC AAATCCTCTA TCCCTCCTCC TCGATCCAGT 
TCCATCCCCT CCTCCCACAT CCCCTCTATT TCTAACGGTT CTTTAAAATA TGCCCCTCCC 
ACTCACAGCA CTAAAGGCCT CTCTATTCCC ACACAGACAC AAAATGGTCG ACCTTCCACT 
TCTTCTTCCT CCTCCTGTTC CCACTCCCCC TTGTCCCCGA CTATGTTGAG TCAGAGTGCG 
AAGAGTATCC GTACCATTCA CACACCCAGC TTCAACAGCT ACAGTCGGCC GCACAACGGA 
AGTGCCGGTA AATCAGCTAT ACCAACGGTA ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_009297305.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295580.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X10, mRNA.

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CloneID	ODa43821
Clone ID Related Accession (Same CDS sequence)	XM_009297305.2
Accession Version	XM_009297305.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297305.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC GCCAACGTGG TCTCACCTAC TAGTCGGATG 
CCAGTGCCAG TTTCTGTCAA GGCGCGGCAG CCATCAAGCA CCTCAGAAAA AGAGAGGGCA 
ACAAAACCGC AAAAACTACA GGACTCTCAG AGGCAGTTCC GCCAGGCTAA TGGAAGTGCT 
AAGAGATCTG GAGGAGATCC CAAATCCACT TCTCCTACCG TGCCTGCTTC TAAAATTCCC 
GCTTTTTCCA CTAGTACGGG AAAAAGCTTG TCCCAGTCTG ATTCTACTAA TAATGTTAAT 
TCTTCTTCTC CTTTATCTTC TTCCCTTCCC ACCAGCAAAT CCTCTATCCC TCCTCCTCGA 
TCCAGTTCCA TCCCCTCCTC CCACATCCCC TCTATTTCTA ACGGTTCTTT AAAATATGCC 
CCTCCCACTC ACAGCACTAA AGGCCTCTCT ATTCCCACAC AGACACAAAA TGGTCGACCT 
TCCACTTCTT CTTCCTCCTC CTGTTCCCAC TCCCCCTTGT CCCCGACTAT GTTGAGTCAG 
AGTGCGAAGA GTATCCGTAC CATTCACACA CCCAGCTTCA ACAGCTACAG TCGGCCGCAC 
AACGGAAGTG CCGGTAAATC AGCTATACCA ACGGTAACTG TCGCTAAGGA TGCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295580.1
CDS	613..5052
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_009297305.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297305.2

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC GCCAACGTGG TCTCACCTAC TAGTCGGATG 
CCAGTGCCAG TTTCTGTCAA GGCGCGGCAG CCATCAAGCA CCTCAGAAAA AGAGAGGGCA 
ACAAAACCGC AAAAACTACA GGACTCTCAG AGGCAGTTCC GCCAGGCTAA TGGAAGTGCT 
AAGAGATCTG GAGGAGATCC CAAATCCACT TCTCCTACCG TGCCTGCTTC TAAAATTCCC 
GCTTTTTCCA CTAGTACGGG AAAAAGCTTG TCCCAGTCTG ATTCTACTAA TAATGTTAAT 
TCTTCTTCTC CTTTATCTTC TTCCCTTCCC ACCAGCAAAT CCTCTATCCC TCCTCCTCGA 
TCCAGTTCCA TCCCCTCCTC CCACATCCCC TCTATTTCTA ACGGTTCTTT AAAATATGCC 
CCTCCCACTC ACAGCACTAA AGGCCTCTCT ATTCCCACAC AGACACAAAA TGGTCGACCT 
TCCACTTCTT CTTCCTCCTC CTGTTCCCAC TCCCCCTTGT CCCCGACTAT GTTGAGTCAG 
AGTGCGAAGA GTATCCGTAC CATTCACACA CCCAGCTTCA ACAGCTACAG TCGGCCGCAC 
AACGGAAGTG CCGGTAAATC AGCTATACCA ACGGTAACTG TCGCTAAGGA TGCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_009297306.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295581.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X11, mRNA.

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CloneID	ODa43822
Clone ID Related Accession (Same CDS sequence)	XM_009297306.2
Accession Version	XM_009297306.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297306.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGAACGCCAA CGTGGTCTCA 
CCTACTAGTC GGATGCCAGT GCCAGTTTCT GTCAAGGCGC GGCAGCCATC AAGCACCTCA 
GAAAAAGAGA GGGCAACAAA ACCGCAAAAA CTACAGGACT CTCAGAGGCA GTTCCGCCAG 
GCTAATGGAA GTGCTAAGAG ATCTGGAGGA GATCCCAAAT CCACTTCTCC TACCGTGCCT 
GCTTCTAAAA TTCCCGCTTT TTCCACTAGT ACGGGAAAAA GCTTGTCCCA GTCTGATTCT 
ACTAATAATG TTAATTCTTC TTCTCCTTTA TCTTCTTCCC TTCCCACCAG CAAATCCTCT 
ATCCCTCCTC CTCGATCCAG TTCCATCCCC TCCTCCCACA TCCCCTCTAT TTCTAACGGT 
TCTTTAAAAT ATGCCCCTCC CACTCACAGC ACTAAAGGCC TCTCTATTCC CACACAGACA 
CAAAATGGTC GACCTTCCAC TTCTTCTTCC TCCTCCTGTT CCCACTCCCC CTTGTCCCCG 
ACTATGTTGA GTCAGAGTGC GAAGAGTATC CGTACCATTC ACACACCCAG CTTCAACAGC 
TACAGTCGGC CGCACAACGG AAGTGCCGGT AAATCAGCTA TACCAACGGT AACTGTCGCT 
AAGGATGCTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295581.1
CDS	613..4947
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_009297306.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297306.2

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGAACGCCAA CGTGGTCTCA 
CCTACTAGTC GGATGCCAGT GCCAGTTTCT GTCAAGGCGC GGCAGCCATC AAGCACCTCA 
GAAAAAGAGA GGGCAACAAA ACCGCAAAAA CTACAGGACT CTCAGAGGCA GTTCCGCCAG 
GCTAATGGAA GTGCTAAGAG ATCTGGAGGA GATCCCAAAT CCACTTCTCC TACCGTGCCT 
GCTTCTAAAA TTCCCGCTTT TTCCACTAGT ACGGGAAAAA GCTTGTCCCA GTCTGATTCT 
ACTAATAATG TTAATTCTTC TTCTCCTTTA TCTTCTTCCC TTCCCACCAG CAAATCCTCT 
ATCCCTCCTC CTCGATCCAG TTCCATCCCC TCCTCCCACA TCCCCTCTAT TTCTAACGGT 
TCTTTAAAAT ATGCCCCTCC CACTCACAGC ACTAAAGGCC TCTCTATTCC CACACAGACA 
CAAAATGGTC GACCTTCCAC TTCTTCTTCC TCCTCCTGTT CCCACTCCCC CTTGTCCCCG 
ACTATGTTGA GTCAGAGTGC GAAGAGTATC CGTACCATTC ACACACCCAG CTTCAACAGC 
TACAGTCGGC CGCACAACGG AAGTGCCGGT AAATCAGCTA TACCAACGGT AACTGTCGCT 
AAGGATGCTG CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470274.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325949.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa151772
Clone ID Related Accession (Same CDS sequence)	XM_021470274.1
Accession Version	XM_021470274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGGT GTCACAAATC 
AATGCTTCTG ACCAATCCCA AAGCCAGCAG CAGGTTTCCA ATAACAAATC AGATGCCCTC 
AAAAACATTG GCCTAGCCAA CTTTTCTGGC ACCCAGCAAC AAAATCAGCT GTCTCGTTCA 
GCCTCACTAA AGTTACATCC AGCCTCTTCA TGGGAAGAAG TTCCTGGTAG CCCAACACAA 
GGGAAGGTTC ATGTGGTCAA GGCTGCTCAA GTGCAGGTTA GCATAGAGGA GAGGGTAGAG 
TCTCCAACAG CTGTTTGCTC ACCAGTCTCT TTTGGAGACT CTGAACCCAT CCTTTCTAAA 
GTGATCCCTG TCGAGACAGC TTTGACAAAT CAAAAGCTTC CATTTGGTTC ATCCAAGGTG 
AAGGAAAGGA AATTTGAGCA AATGGAGGAA AATAATGAGG CTTTTCTCAG TCCCGATTTG 
TCAGGAGAAG AGCCACCTCC TCCACCACCT GATAACTTTG CCTTTATGAT AACCAACACC 
AAGGTTCAAG CCCTCTCCAC TGGAGAGTAC CATCAGCTTG TCAACGCCAA GAAAGGAAGT 
GTACAGACAG TGACTTTTGG CAACAAACAA CCAAGTGCTT CTTACAGTGG CATGGCATCA 
GGAGGTGATG CTAATACCAA TGGCCCTCCT GGAGCTGATA ACACTAGTAA AAAGCCAGTT 
ATCATAATCT TTGATGAGCC AATGGACATC CGTTCAGCTT ACAAGCGCCT CTCTACCGTT 
TTCGAATGTG AGGAGGAGCT AGATCGGATG CTTGCAGAAG AAAGAATAGA TGAGGAGAGT 
GAGGAAACTG AGGAAGAAGA ATGGACGAGA GGAGAGAAGG TCAAACAGAA TGGAGTTGAA 
AGACAGTCTG TAAAAAGAAC TCTTGCAGAT GTCACAGATG GTGAAGTCAA GAACTCTTCA 
TCATCCTTAC TGGTTGCTGG TGATGCAAAG CAAGATGGGA AGAAAAAGTT TAAGTTGAAA 
TTTCCCAAGA AACAGCTAGC AGCATTAACA CAAGCTATTC GGGCTGGAAG TAAAACAGGC 
AAAAAGACTT TGCAAGTTGT CGTCTATGAG GATGAGGAAG AGTCCGATGG CACTGTCAAG 
CAACATAAAG AGACCAAGAG GTATGAAATT GCTAGCTCCA AAACAGAATC CTCAAAATCA 
AATACACAGG AATCTAATGG ACGAACTGAT GAGATTCGTA AGAGCACCTA CAAAACACTT 
GACAGTCTAG AGCAAACAAT AAAGCAGCTA GAGAATACAA TTTGCGAAAT GGGACCAACG 
TCTTCGGAAG ACACTGAAAG CCTAACGAAT TCTGAAACTC AAGAAAACAA AGTAGTGGAG 
AATTCAGGCC ACTCAGAGAA GGGACTTTCT TCCAAAACCT TGGTTCCTAG GTTGTCTGCT 
GCCAGTAAGG GTCCTAGTCT CCGAAAGAAG ACCAAACCAC AGCTCCTTCC TCGGCCTACC 
AACATACCAA CTACAGGGGT CTCAGTAACA CCAGTCGCTA CCCACCAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGGCTA ATGGAAGTGC TAAGAGATCT GGAGGAGATC CCAAATCCAC TTCTCCTACC 
GTGCCTGCTT CTAAAATTCC CGCTTTTTCC ACTAGTACGG GAAAAAGCTT GTCCCAGTCT 
GATTCTACTA ATAATGTTAA TTCTTCTTCT CCTTTATCTT CTTCCCTTCC CACCAGCAAA 
TCCTCTATCC CTCCTCCTCG ATCCAGTTCC ATCCCCTCCT CCCACATCCC CTCTATTTCT 
AACGGTTCTT TAAAATATGC CCCTCCCACT CACAGCACTA AAGGCCTCTC TATTCCCACA 
CAGACACAAA ATGGTCGACC TTCCACTTCT TCTTCCTCCT CCTGTTCCCA CTCCCCCTTG 
TCCCCGACTA TGTTGAGTCA GAGTGCGAAG AGTATCCGTA CCATTCACAC ACCCAGCTTC 
AACAGCTACA GTCGGCCGCA CAACGGAAGT GCCGGTAAAT CAGCTATACC AACGGTAACT 
GTCGCTAAGG ATGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325949.1
CDS	596..6796
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470274.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470274.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGG TTTTCCAGAA GTGCTCTGTT AAGGAGGTAG GGCTCAAGGG TTTGGTAGAG 
CCACAGATCA GAGAGCTAAG ACCTGGGGTC TTACTGCCCC TTAAAGATAA GAAGAACACA 
GAGAACCAAG ATGACAAGAA TACTGACACA GATGAGAATG GCAACAATAC TATTCGTCAA 
AGTCCTGGGG TCATCTACTA TGTCACAGCT CAGATCTCCA AGGGAACTCC ACCACCGTCA 
GAGGAATCAA CTGAGCGTAG AGATGCATCG CAGTCTTTCC CATCACAGGT GTCACAAATC 
AATGCTTCTG ACCAATCCCA AAGCCAGCAG CAGGTTTCCA ATAACAAATC AGATGCCCTC 
AAAAACATTG GCCTAGCCAA CTTTTCTGGC ACCCAGCAAC AAAATCAGCT GTCTCGTTCA 
GCCTCACTAA AGTTACATCC AGCCTCTTCA TGGGAAGAAG TTCCTGGTAG CCCAACACAA 
GGGAAGGTTC ATGTGGTCAA GGCTGCTCAA GTGCAGGTTA GCATAGAGGA GAGGGTAGAG 
TCTCCAACAG CTGTTTGCTC ACCAGTCTCT TTTGGAGACT CTGAACCCAT CCTTTCTAAA 
GTGATCCCTG TCGAGACAGC TTTGACAAAT CAAAAGCTTC CATTTGGTTC ATCCAAGGTG 
AAGGAAAGGA AATTTGAGCA AATGGAGGAA AATAATGAGG CTTTTCTCAG TCCCGATTTG 
TCAGGAGAAG AGCCACCTCC TCCACCACCT GATAACTTTG CCTTTATGAT AACCAACACC 
AAGGTTCAAG CCCTCTCCAC TGGAGAGTAC CATCAGCTTG TCAACGCCAA GAAAGGAAGT 
GTACAGACAG TGACTTTTGG CAACAAACAA CCAAGTGCTT CTTACAGTGG CATGGCATCA 
GGAGGTGATG CTAATACCAA TGGCCCTCCT GGAGCTGATA ACACTAGTAA AAAGCCAGTT 
ATCATAATCT TTGATGAGCC AATGGACATC CGTTCAGCTT ACAAGCGCCT CTCTACCGTT 
TTCGAATGTG AGGAGGAGCT AGATCGGATG CTTGCAGAAG AAAGAATAGA TGAGGAGAGT 
GAGGAAACTG AGGAAGAAGA ATGGACGAGA GGAGAGAAGG TCAAACAGAA TGGAGTTGAA 
AGACAGTCTG TAAAAAGAAC TCTTGCAGAT GTCACAGATG GTGAAGTCAA GAACTCTTCA 
TCATCCTTAC TGGTTGCTGG TGATGCAAAG CAAGATGGGA AGAAAAAGTT TAAGTTGAAA 
TTTCCCAAGA AACAGCTAGC AGCATTAACA CAAGCTATTC GGGCTGGAAG TAAAACAGGC 
AAAAAGACTT TGCAAGTTGT CGTCTATGAG GATGAGGAAG AGTCCGATGG CACTGTCAAG 
CAACATAAAG AGACCAAGAG GTATGAAATT GCTAGCTCCA AAACAGAATC CTCAAAATCA 
AATACACAGG AATCTAATGG ACGAACTGAT GAGATTCGTA AGAGCACCTA CAAAACACTT 
GACAGTCTAG AGCAAACAAT AAAGCAGCTA GAGAATACAA TTTGCGAAAT GGGACCAACG 
TCTTCGGAAG ACACTGAAAG CCTAACGAAT TCTGAAACTC AAGAAAACAA AGTAGTGGAG 
AATTCAGGCC ACTCAGAGAA GGGACTTTCT TCCAAAACCT TGGTTCCTAG GTTGTCTGCT 
GCCAGTAAGG GTCCTAGTCT CCGAAAGAAG ACCAAACCAC AGCTCCTTCC TCGGCCTACC 
AACATACCAA CTACAGGGGT CTCAGTAACA CCAGTCGCTA CCCACCAGAA CGCCAACGTG 
GTCTCACCTA CTAGTCGGAT GCCAGTGCCA GTTTCTGTCA AGGCGCGGCA GCCATCAAGC 
ACCTCAGAAA AAGAGAGGGC AACAAAACCG CAAAAACTAC AGGACTCTCA GAGGCAGTTC 
CGCCAGGCTA ATGGAAGTGC TAAGAGATCT GGAGGAGATC CCAAATCCAC TTCTCCTACC 
GTGCCTGCTT CTAAAATTCC CGCTTTTTCC ACTAGTACGG GAAAAAGCTT GTCCCAGTCT 
GATTCTACTA ATAATGTTAA TTCTTCTTCT CCTTTATCTT CTTCCCTTCC CACCAGCAAA 
TCCTCTATCC CTCCTCCTCG ATCCAGTTCC ATCCCCTCCT CCCACATCCC CTCTATTTCT 
AACGGTTCTT TAAAATATGC CCCTCCCACT CACAGCACTA AAGGCCTCTC TATTCCCACA 
CAGACACAAA ATGGTCGACC TTCCACTTCT TCTTCCTCCT CCTGTTCCCA CTCCCCCTTG 
TCCCCGACTA TGTTGAGTCA GAGTGCGAAG AGTATCCGTA CCATTCACAC ACCCAGCTTC 
AACAGCTACA GTCGGCCGCA CAACGGAAGT GCCGGTAAAT CAGCTATACC AACGGTAACT 
GTCGCTAAGG ATGCTGCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470275.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325950.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X3, mRNA.

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CloneID	ODa151773
Clone ID Related Accession (Same CDS sequence)	XM_021470275.1
Accession Version	XM_021470275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGA ACACAGAGAA CCAAGATGAC AAGAATACTG ACACAGATGA GAATGGCAAC 
AATACTATTC GTCAAAGTCC TGGGGTCATC TACTATGTCA CAGCTCAGAT CTCCAAGGGA 
ACTCCACCAC CGTCAGAGGA ATCAACTGAG CGTAGAGATG CATCGCAGTC TTTCCCATCA 
CAGGTGTCAC AAATCAATGC TTCTGACCAA TCCCAAAGCC AGCAGCAGGT TTCCAATAAC 
AAATCAGATG CCCTCAAAAA CATTGGCCTA GCCAACTTTT CTGGCACCCA GCAACAAAAT 
CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA CATCCAGCCT CTTCATGGGA AGAAGTTCCT 
GGTAGCCCAA CACAAGGGAA GGTTCATGTG GTCAAGGCTG CTCAAGTGCA GGTTAGCATA 
GAGGAGAGGG TAGAGTCTCC AACAGCTGTT TGCTCACCAG TCTCTTTTGG AGACTCTGAA 
CCCATCCTTT CTAAAGTGAT CCCTGTCGAG ACAGCTTTGA CAAATCAAAA GCTTCCATTT 
GGTTCATCCA AGGTGAAGGA AAGGAAATTT GAGCAAATGG AGGAAAATAA TGAGGCTTTT 
CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA CCTCCTCCAC CACCTGATAA CTTTGCCTTT 
ATGATAACCA ACACCAAGGT TCAAGCCCTC TCCACTGGAG AGTACCATCA GCTTGTCAAC 
GCCAAGAAAG GAAGTGTACA GACAGTGACT TTTGGCAACA AACAACCAAG TGCTTCTTAC 
AGTGGCATGG CATCAGGAGG TGATGCTAAT ACCAATGGCC CTCCTGGAGC TGATAACACT 
AGTAAAAAGC CAGTTATCAT AATCTTTGAT GAGCCAATGG ACATCCGTTC AGCTTACAAG 
CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG GAGCTAGATC GGATGCTTGC AGAAGAAAGA 
ATAGATGAGG AGAGTGAGGA AACTGAGGAA GAAGAATGGA CGAGAGGAGA GAAGGTCAAA 
CAGAATGGAG TTGAAAGACA GTCTGTAAAA AGAACTCTTG CAGATGTCAC AGATGGTGAA 
GTCAAGAACT CTTCATCATC CTTACTGGTT GCTGGTGATG CAAAGCAAGA TGGGAAGAAA 
AAGTTTAAGT TGAAATTTCC CAAGAAACAG CTAGCAGCAT TAACACAAGC TATTCGGGCT 
GGAAGTAAAA CAGGCAAAAA GACTTTGCAA GTTGTCGTCT ATGAGGATGA GGAAGAGTCC 
GATGGCACTG TCAAGCAACA TAAAGAGACC AAGAGGTATG AAATTGCTAG CTCCAAAACA 
GAATCCTCAA AATCAAATAC ACAGGAATCT AATGGACGAA CTGATGAGAT TCGTAAGAGC 
ACCTACAAAA CACTTGACAG TCTAGAGCAA ACAATAAAGC AGCTAGAGAA TACAATTTGC 
GAAATGGGAC CAACGTCTTC GGAAGACACT GAAAGCCTAA CGAATTCTGA AACTCAAGAA 
AACAAAGTAG TGGAGAATTC AGGCCACTCA GAGAAGGGAC TTTCTTCCAA AACCTTGGTT 
CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT AGTCTCCGAA AGAAGACCAA ACCACAGCTC 
CTTCCTCGGC CTACCAACAT ACCAACTACA GGGGTCTCAG TAACACCAGT CGCTACCCAC 
CAGAACGCCA ACGTGGTCTC ACCTACTAGT CGGATGCCAG TGCCAGTTTC TGTCAAGGCG 
CGGCAGCCAT CAAGCACCTC AGAAAAAGAG AGGGCAACAA AACCGCAAAA ACTACAGGAC 
TCTCAGAGGC AGTTCCGCCA GGCTAATGGA AGTGCTAAGA GATCTGGAGG AGATCCCAAA 
TCCACTTCTC CTACCGTGCC TGCTTCTAAA ATTCCCGCTT TTTCCACTAG TACGGGAAAA 
AGCTTGTCCC AGTCTGATTC TACTAATAAT GTTAATTCTT CTTCTCCTTT ATCTTCTTCC 
CTTCCCACCA GCAAATCCTC TATCCCTCCT CCTCGATCCA GTTCCATCCC CTCCTCCCAC 
ATCCCCTCTA TTTCTAACGG TTCTTTAAAA TATGCCCCTC CCACTCACAG CACTAAAGGC 
CTCTCTATTC CCACACAGAC ACAAAATGGT CGACCTTCCA CTTCTTCTTC CTCCTCCTGT 
TCCCACTCCC CCTTGTCCCC GACTATGTTG AGTCAGAGTG CGAAGAGTAT CCGTACCATT 
CACACACCCA GCTTCAACAG CTACAGTCGG CCGCACAACG GAAGTGCCGG TAAATCAGCT 
ATACCAACGG TAACTGTCGC TAAGGATGCT GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325950.1
CDS	596..6691
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470275.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470275.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG AGAGAGAATG 
AAGGCCATGG AGGAGCAAAT AGCCAGTCTG ACTGGTCTTG TGCAGCACGC TCTCTTAAAG 
AGTAACAGCA CTAACAGCAA CAAGGAGCAC TCCAGCGAAA GACCAGTGAA GTCTGGATCA 
CCAGCTCACA GCACATCTAG CACAGGAGGC TCTCCAGTAT TAGCACCAAA GGTTGTTGCT 
GCTCCTCCTG AGCGTAGCCC CGCCCCCACA CCACCTTTAG GACCTGCCCC TTTGCATGTT 
AACCTTCATC TGTTCAGGAA GAATGTCTCT GAGCTCCGCC TCCAACTACA ACATATGAGA 
CAACTACAGC TTCAGAATCA GGAGTGTGTA CGTGCTCAGA TAAAGCGCGC AGAACAGGAG 
ATCGGTGTGA AACTGGCTGA AATGCTGAGA CGACAGGATG ATCCGACACA GAAACAGAGG 
ATGCTTGTGG AGGAAGAGAG ACACCGATAT CTCAGCATGC AGGAAAGAGT TCTCACACAG 
CTGGGGGATC TGGAGCAGTT TGTGGAGACT CTGAGGACTG ATTCGGGTCC TGCTGCGTCT 
CAGAGAGCAG TGACGCTGAA GGAGGTTGAG GATGGGGCTG TCAGCCTGAG GAAGGTGGGA 
GAAAACCTGG CTGGACTCAA AGGTGAGTTT CCGGCTCTTC AGTCCCGCAT GCGCTCAGTG 
CTCAGGGTTG AAGTAGAGGC TGTAAAGTTC CTCAAAGAAG AGCCACACAA ACTGGACAGC 
ATGTTAAAGA GAGTCCGGAC CCTCACTGAC ACACTCAGCA ACCTTCGCAG GTGTGCCACC 
GATAACCTGC TTAAACCCAC TGATCAGGTT TGCTCAACAC CTCCTGCCTC TGAAAAACCA 
GAACTTATTG AATCCCCAGA ACCTGCACCG GTCACACCTC AGAGCTCCAT CGTGAGGTCA 
GAGGTTATGA CCTCAAACCC TGTGGTGATC CACCATGCTC AGGCCTCTCC AGTAGCATCA 
CAACAATCCC AGCAATCAAC AGCACCTTCA CCCATCCCTA CACAAAGAAG AGAGTCTACA 
CCGTCACCCA CTAAACAGCT CAGAAAACCC TCACTGGAAC AAACACAAAA CAACACCAGT 
AATGGAGTTT CTGCTAATGG CGTACATGGC AAGGGTCCTT CTCCAAACCT GATCATTGAG 
GAGATCCACG CCAGCCACAG CAAGAACAAG AACAGAGCTG TGTCCATAGA GGCTGCAGAG 
AAGGAATGGG AGGAGAAAAG GCAGAACTTG GGTCATTATG ATGGACAGGA GTTTGAGCGG 
ATTCTCCAGG AAGCAGAAGC AAACATGCTG AGGGGAATAC CAAACCTCGA GGTGCCCAGT 
CAACTTGAGG GTTCTCCTGC TCCACCTGCC ATGCTTCCTG CCTCTCTGGA GGAGGTAGTA 
GAAATAAACG AACCAGTAGC AGCACCAAGA GAACACGATG ACAATCAGCC AAACTCTGAG 
AAACCAGCTA AGACTGCTTC AGAGAAACCT CCCAAACCTC TGGAGAAACC AGTTAAGCCA 
GCCTTGGAGA AACCTCTTAA ACCCAGCCTA TCCATCAAAT CCAACCTAGA GAGACAGAGT 
AAGACTCAGG AAAAAGTTAA ACTTCAGAGC ACAGAGAAAA CCAACAAGTC TCCTCCTCCA 
CCCCCACCTC GACGCAACTA CACCACCAAC ACCGGCATGA CCACCACCCG CTCTGGAGAG 
GTCATTTACA CCAGCAGGAA AGAGTCTGTA ACGGAAGGTG AAGAGGAGTC CAACATTTCA 
ACAATGTCTC AGCCTGAACC AAAGACCCCC CCAGAGGTAA AGCCCAAACC CATCACCCCT 
CCACCTATCT CTGCCTCAGC CATCACTGAA GAAGAGGACG AAGGAGACAA GATTATGGCA 
GAGCTACAGA ACACAGAGAA CCAAGATGAC AAGAATACTG ACACAGATGA GAATGGCAAC 
AATACTATTC GTCAAAGTCC TGGGGTCATC TACTATGTCA CAGCTCAGAT CTCCAAGGGA 
ACTCCACCAC CGTCAGAGGA ATCAACTGAG CGTAGAGATG CATCGCAGTC TTTCCCATCA 
CAGGTGTCAC AAATCAATGC TTCTGACCAA TCCCAAAGCC AGCAGCAGGT TTCCAATAAC 
AAATCAGATG CCCTCAAAAA CATTGGCCTA GCCAACTTTT CTGGCACCCA GCAACAAAAT 
CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA CATCCAGCCT CTTCATGGGA AGAAGTTCCT 
GGTAGCCCAA CACAAGGGAA GGTTCATGTG GTCAAGGCTG CTCAAGTGCA GGTTAGCATA 
GAGGAGAGGG TAGAGTCTCC AACAGCTGTT TGCTCACCAG TCTCTTTTGG AGACTCTGAA 
CCCATCCTTT CTAAAGTGAT CCCTGTCGAG ACAGCTTTGA CAAATCAAAA GCTTCCATTT 
GGTTCATCCA AGGTGAAGGA AAGGAAATTT GAGCAAATGG AGGAAAATAA TGAGGCTTTT 
CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA CCTCCTCCAC CACCTGATAA CTTTGCCTTT 
ATGATAACCA ACACCAAGGT TCAAGCCCTC TCCACTGGAG AGTACCATCA GCTTGTCAAC 
GCCAAGAAAG GAAGTGTACA GACAGTGACT TTTGGCAACA AACAACCAAG TGCTTCTTAC 
AGTGGCATGG CATCAGGAGG TGATGCTAAT ACCAATGGCC CTCCTGGAGC TGATAACACT 
AGTAAAAAGC CAGTTATCAT AATCTTTGAT GAGCCAATGG ACATCCGTTC AGCTTACAAG 
CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG GAGCTAGATC GGATGCTTGC AGAAGAAAGA 
ATAGATGAGG AGAGTGAGGA AACTGAGGAA GAAGAATGGA CGAGAGGAGA GAAGGTCAAA 
CAGAATGGAG TTGAAAGACA GTCTGTAAAA AGAACTCTTG CAGATGTCAC AGATGGTGAA 
GTCAAGAACT CTTCATCATC CTTACTGGTT GCTGGTGATG CAAAGCAAGA TGGGAAGAAA 
AAGTTTAAGT TGAAATTTCC CAAGAAACAG CTAGCAGCAT TAACACAAGC TATTCGGGCT 
GGAAGTAAAA CAGGCAAAAA GACTTTGCAA GTTGTCGTCT ATGAGGATGA GGAAGAGTCC 
GATGGCACTG TCAAGCAACA TAAAGAGACC AAGAGGTATG AAATTGCTAG CTCCAAAACA 
GAATCCTCAA AATCAAATAC ACAGGAATCT AATGGACGAA CTGATGAGAT TCGTAAGAGC 
ACCTACAAAA CACTTGACAG TCTAGAGCAA ACAATAAAGC AGCTAGAGAA TACAATTTGC 
GAAATGGGAC CAACGTCTTC GGAAGACACT GAAAGCCTAA CGAATTCTGA AACTCAAGAA 
AACAAAGTAG TGGAGAATTC AGGCCACTCA GAGAAGGGAC TTTCTTCCAA AACCTTGGTT 
CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT AGTCTCCGAA AGAAGACCAA ACCACAGCTC 
CTTCCTCGGC CTACCAACAT ACCAACTACA GGGGTCTCAG TAACACCAGT CGCTACCCAC 
CAGAACGCCA ACGTGGTCTC ACCTACTAGT CGGATGCCAG TGCCAGTTTC TGTCAAGGCG 
CGGCAGCCAT CAAGCACCTC AGAAAAAGAG AGGGCAACAA AACCGCAAAA ACTACAGGAC 
TCTCAGAGGC AGTTCCGCCA GGCTAATGGA AGTGCTAAGA GATCTGGAGG AGATCCCAAA 
TCCACTTCTC CTACCGTGCC TGCTTCTAAA ATTCCCGCTT TTTCCACTAG TACGGGAAAA 
AGCTTGTCCC AGTCTGATTC TACTAATAAT GTTAATTCTT CTTCTCCTTT ATCTTCTTCC 
CTTCCCACCA GCAAATCCTC TATCCCTCCT CCTCGATCCA GTTCCATCCC CTCCTCCCAC 
ATCCCCTCTA TTTCTAACGG TTCTTTAAAA TATGCCCCTC CCACTCACAG CACTAAAGGC 
CTCTCTATTC CCACACAGAC ACAAAATGGT CGACCTTCCA CTTCTTCTTC CTCCTCCTGT 
TCCCACTCCC CCTTGTCCCC GACTATGTTG AGTCAGAGTG CGAAGAGTAT CCGTACCATT 
CACACACCCA GCTTCAACAG CTACAGTCGG CCGCACAACG GAAGTGCCGG TAAATCAGCT 
ATACCAACGG TAACTGTCGC TAAGGATGCT GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_021470276.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325951.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X4, mRNA.

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CloneID	ODa151774
Clone ID Related Accession (Same CDS sequence)	XM_021470276.1
Accession Version	XM_021470276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG CGAAAGACCA 
GTGAAGTCTG GATCACCAGC TCACAGCACA TCTAGCACAG GAGGCTCTCC AGTATTAGCA 
CCAAAGGTTG TTGCTGCTCC TCCTGAGCGT AGCCCCGCCC CCACACCACC TTTAGGACCT 
GCCCCTTTGC ATGTTAACCT TCATCTGTTC AGGAAGAATG TCTCTGAGCT CCGCCTCCAA 
CTACAACATA TGAGACAACT ACAGCTTCAG AATCAGGAGT GTGTACGTGC TCAGATAAAG 
CGCGCAGAAC AGGAGATCGG TGTGAAACTG GCTGAAATGC TGAGACGACA GGATGATCCG 
ACACAGAAAC AGAGGATGCT TGTGGAGGAA GAGAGACACC GATATCTCAG CATGCAGGAA 
AGAGTTCTCA CACAGCTGGG GGATCTGGAG CAGTTTGTGG AGACTCTGAG GACTGATTCG 
GGTCCTGCTG CGTCTCAGAG AGCAGTGACG CTGAAGGAGG TTGAGGATGG GGCTGTCAGC 
CTGAGGAAGG TGGGAGAAAA CCTGGCTGGA CTCAAAGGTG AGTTTCCGGC TCTTCAGTCC 
CGCATGCGCT CAGTGCTCAG GGTTGAAGTA GAGGCTGTAA AGTTCCTCAA AGAAGAGCCA 
CACAAACTGG ACAGCATGTT AAAGAGAGTC CGGACCCTCA CTGACACACT CAGCAACCTT 
CGCAGGTGTG CCACCGATAA CCTGCTTAAA CCCACTGATC AGGTTTGCTC AACACCTCCT 
GCCTCTGAAA AACCAGAACT TATTGAATCC CCAGAACCTG CACCGGTCAC ACCTCAGAGC 
TCCATCGTGA GGTCAGAGGT TATGACCTCA AACCCTGTGG TGATCCACCA TGCTCAGGCC 
TCTCCAGTAG CATCACAACA ATCCCAGCAA TCAACAGCAC CTTCACCCAT CCCTACACAA 
AGAAGAGAGT CTACACCGTC ACCCACTAAA CAGCTCAGAA AACCCTCACT GGAACAAACA 
CAAAACAACA CCAGTAATGG AGTTTCTGCT AATGGCGTAC ATGGCAAGGG TCCTTCTCCA 
AACCTGATCA TTGAGGAGAT CCACGCCAGC CACAGCAAGA ACAAGAACAG AGCTGTGTCC 
ATAGAGGCTG CAGAGAAGGA ATGGGAGGAG AAAAGGCAGA ACTTGGGTCA TTATGATGGA 
CAGGAGTTTG AGCGGATTCT CCAGGAAGCA GAAGCAAACA TGCTGAGGGG AATACCAAAC 
CTCGAGGTGC CCAGTCAACT TGAGGGTTCT CCTGCTCCAC CTGCCATGCT TCCTGCCTCT 
CTGGAGGAGG TAGTAGAAAT AAACGAACCA GTAGCAGCAC CAAGAGAACA CGATGACAAT 
CAGCCAAACT CTGAGAAACC AGCTAAGACT GCTTCAGAGA AACCTCCCAA ACCTCTGGAG 
AAACCAGTTA AGCCAGCCTT GGAGAAACCT CTTAAACCCA GCCTATCCAT CAAATCCAAC 
CTAGAGAGAC AGAGTAAGAC TCAGGAAAAA GTTAAACTTC AGAGCACAGA GAAAACCAAC 
AAGTCTCCTC CTCCACCCCC ACCTCGACGC AACTACACCA CCAACACCGG CATGACCACC 
ACCCGCTCTG GAGAGGTCAT TTACACCAGC AGGAAAGAGT CTGTAACGGA AGGTGAAGAG 
GAGTCCAACA TTTCAACAAT GTCTCAGCCT GAACCAAAGA CCCCCCCAGA GGTAAAGCCC 
AAACCCATCA CCCCTCCACC TATCTCTGCC TCAGCCATCA CTGAAGAAGA GGACGAAGGA 
GACAAGATTA TGGCAGAGCT ACAGGTTTTC CAGAAGTGCT CTGTTAAGGA GGTAGGGCTC 
AAGGGTTTGG TAGAGCCACA GATCAGAGAG CTAAGACCTG GGGTCTTACT GCCCCTTAAA 
GATAAGAAGA ACACAGAGAA CCAAGATGAC AAGAATACTG ACACAGATGA GAATGGCAAC 
AATACTATTC GTCAAAGTCC TGGGGTCATC TACTATGTCA CAGCTCAGAT CTCCAAGGGA 
ACTCCACCAC CGTCAGAGGA ATCAACTGAG CGTAGAGATG CATCGCAGTC TTTCCCATCA 
CAGGTGTCAC AAATCAATGC TTCTGACCAA TCCCAAAGCC AGCAGCAGGT TTCCAATAAC 
AAATCAGATG CCCTCAAAAA CATTGGCCTA GCCAACTTTT CTGGCACCCA GCAACAAAAT 
CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA CATCCAGCCT CTTCATGGGA AGAAGTTCCT 
GGTAGCCCAA CACAAGGGAA GGTTCATGTG GTCAAGGCTG CTCAAGTGCA GGTTAGCATA 
GAGGAGAGGG TAGAGTCTCC AACAGCTGTT TGCTCACCAG TCTCTTTTGG AGACTCTGAA 
CCCATCCTTT CTAAAGTGAT CCCTGTCGAG ACAGCTTTGA CAAATCAAAA GCTTCCATTT 
GGTTCATCCA AGGTGAAGGA AAGGAAATTT GAGCAAATGG AGGAAAATAA TGAGGCTTTT 
CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA CCTCCTCCAC CACCTGATAA CTTTGCCTTT 
ATGATAACCA ACACCAAGGT TCAAGCCCTC TCCACTGGAG AGTACCATCA GCTTGTCAAC 
GCCAAGAAAG GAAGTGTACA GACAGTGACT TTTGGCAACA AACAACCAAG TGCTTCTTAC 
AGTGGCATGG CATCAGGAGG TGATGCTAAT ACCAATGGCC CTCCTGGAGC TGATAACACT 
AGTAAAAAGC CAGTTATCAT AATCTTTGAT GAGCCAATGG ACATCCGTTC AGCTTACAAG 
CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG GAGCTAGATC GGATGCTTGC AGAAGAAAGA 
ATAGATGAGG AGAGTGAGGA AACTGAGGAA GAAGAATGGA CGAGAGGAGA GAAGGTCAAA 
CAGAATGGAG TTGAAAGACA GTCTGTAAAA AGAACTCTTG CAGATGTCAC AGATGGTGAA 
GTCAAGAACT CTTCATCATC CTTACTGGTT GCTGGTGATG CAAAGCAAGA TGGGAAGAAA 
AAGTTTAAGT TGAAATTTCC CAAGAAACAG CTAGCAGCAT TAACACAAGC TATTCGGGCT 
GGAAGTAAAA CAGGCAAAAA GACTTTGCAA GTTGTCGTCT ATGAGGATGA GGAAGAGTCC 
GATGGCACTG TCAAGCAACA TAAAGAGACC AAGAGGTATG AAATTGCTAG CTCCAAAACA 
GAATCCTCAA AATCAAATAC ACAGGAATCT AATGGACGAA CTGATGAGAT TCGTAAGAGC 
ACCTACAAAA CACTTGACAG TCTAGAGCAA ACAATAAAGC AGCTAGAGAA TACAATTTGC 
GAAATGGGAC CAACGTCTTC GGAAGACACT GAAAGCCTAA CGAATTCTGA AACTCAAGAA 
AACAAAGTAG TGGAGAATTC AGGCCACTCA GAGAAGGGAC TTTCTTCCAA AACCTTGGTT 
CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT AGTCTCCGAA AGAAGACCAA ACCACAGCTC 
CTTCCTCGGC CTACCAACAT ACCAACTACA GGGGTCTCAG TAACACCAGT CGCTACCCAC 
CAGAACGCCA ACGTGGTCTC ACCTACTAGT CGGATGCCAG TGCCAGTTTC TGTCAAGGCG 
CGGCAGCCAT CAAGCACCTC AGAAAAAGAG AGGGCAACAA AACCGCAAAA ACTACAGGAC 
TCTCAGAGGC AGTTCCGCCA GGCTAATGGA AGTGCTAAGA GATCTGGAGG AGATCCCAAA 
TCCACTTCTC CTACCGTGCC TGCTTCTAAA ATTCCCGCTT TTTCCACTAG TACGGGAAAA 
AGCTTGTCCC AGTCTGATTC TACTAATAAT GTTAATTCTT CTTCTCCTTT ATCTTCTTCC 
CTTCCCACCA GCAAATCCTC TATCCCTCCT CCTCGATCCA GTTCCATCCC CTCCTCCCAC 
ATCCCCTCTA TTTCTAACGG TTCTTTAAAA TATGCCCCTC CCACTCACAG CACTAAAGGC 
CTCTCTATTC CCACACAGAC ACAAAATGGT CGACCTTCCA CTTCTTCTTC CTCCTCCTGT 
TCCCACTCCC CCTTGTCCCC GACTATGTTG AGTCAGAGTG CGAAGAGTAT CCGTACCATT 
CACACACCCA GCTTCAACAG CTACAGTCGG CCGCACAACG GAAGTGCCGG TAAATCAGCT 
ATACCAACGG TAACTGTCGC TAAGGATGCT GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325951.1
CDS	596..6691
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQSFQRMLSALHSEIDIQRYLLKIQAAGSRRTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_021470276.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470276.1

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GTCATTTCAG 
AGGATGCTGT CGGCTTTGCA CTCTGAGATA GACATTCAAA GATATCTGCT GAAAATCCAG 
GCTGCTGGTT CACGCAGAAC CCGGGGCTCT AAGCAGAGTG TGGGGTGTCC GAGTCCAGTG 
CCTGTTTTTG TGGATCAGGT AGAGCACGGC TCACTGGTCT CTCTAGATGC TCTGGAGGTC 
ATGTCTGAGT GTGATATGCC CCTCGCCTTC ACCCGAGGCT CCCGCTCTCG AGCCAGTCTG 
CCAGTGGTCC GCTCTACTAA CCAGACCAAG GATCGCTCAC TAGGAGTGCT GTATCTGCAG 
TATGGGGACG ACACAAAGCA GATCCGCATG CCTAATGAGA TAACAAGTGT TGACACAATC 
AGAGCCCTGT TCGTTAGTGC CTTCCCTCAT CAGCTCAACA TGAAGATGCT GGAGTCGCCC 
AGCACTGCCA TCTACGTCAA AGATGAGACC AGAAATGTGT ATTATGAACT CACCGATGTC 
AGGAGCATCA CAGATCACTC CTGCCTGAAA GTGTATCATA AAGACCCAGC ACACGCCTTC 
AGTCACGGAC CCCGGCCGAG CAATGGAGAC ACAAGGGTTC ACAGGGAAAT GATATTTGCT 
GGACGGGATG GTCACCCACT CCGGAACCCC CCAATGGGTC CTCCACCCCC CCACATGCAA 
GGCTCCATGT CACCCACTAC ACCTCACGGG ATGCCTCCCT CTCCATCTCG AATTCCCTTT 
GGCCCGCGAA ACCCAGGTGG AGGTGTTGCC ACAATGCCAC GTGAGCGAGC CGGACACACA 
CTCCCAGCCC CTGCCCGAGC ATCCTCCCCC TGCCCGAGTG CCATCTTGGA GCGGCGGGAT 
GTCAAACCTG ACGAGGATGT AGCTGCGAAG ACCATGTCTC TGAACACCAG AGCTGAAAGT 
CATTACGCCG ACCCTTATAT GTTACTTCAC CATGCCCCGC CCCCTGACAC AGTAGACCAC 
GCCTACCACC GTGCTACCAT GCGCTCTTAC AGCAACCCCA GCGTTCCCTT GGAGCCCCCA 
GACCATCTCT TCCGACAGAA AAGCAGGAAT AGCCAACTCC CCATGCTTGG CTCCAAAACC 
CCGCCCCCTT CCCCGCACCG AATGGGTGAG ATGAGAATGG TGGACATCCA TACGGTCCAA 
CCGCAGAGCT CCATGACTCT GGAAAGGGCT TCACCTGTTA GACAATCATT TCGGAAGGAG 
GCTCCTCCTA TGGATGCCAT GGTAAAGGCA AGAGGGGGAA TGGGGTCTCC AGCCACCCCC 
GATCTTCAGG TTCACAATCC TCTGGCGCCC CCGACAGACC CTCAAACACG CGAAAGACCA 
GTGAAGTCTG GATCACCAGC TCACAGCACA TCTAGCACAG GAGGCTCTCC AGTATTAGCA 
CCAAAGGTTG TTGCTGCTCC TCCTGAGCGT AGCCCCGCCC CCACACCACC TTTAGGACCT 
GCCCCTTTGC ATGTTAACCT TCATCTGTTC AGGAAGAATG TCTCTGAGCT CCGCCTCCAA 
CTACAACATA TGAGACAACT ACAGCTTCAG AATCAGGAGT GTGTACGTGC TCAGATAAAG 
CGCGCAGAAC AGGAGATCGG TGTGAAACTG GCTGAAATGC TGAGACGACA GGATGATCCG 
ACACAGAAAC AGAGGATGCT TGTGGAGGAA GAGAGACACC GATATCTCAG CATGCAGGAA 
AGAGTTCTCA CACAGCTGGG GGATCTGGAG CAGTTTGTGG AGACTCTGAG GACTGATTCG 
GGTCCTGCTG CGTCTCAGAG AGCAGTGACG CTGAAGGAGG TTGAGGATGG GGCTGTCAGC 
CTGAGGAAGG TGGGAGAAAA CCTGGCTGGA CTCAAAGGTG AGTTTCCGGC TCTTCAGTCC 
CGCATGCGCT CAGTGCTCAG GGTTGAAGTA GAGGCTGTAA AGTTCCTCAA AGAAGAGCCA 
CACAAACTGG ACAGCATGTT AAAGAGAGTC CGGACCCTCA CTGACACACT CAGCAACCTT 
CGCAGGTGTG CCACCGATAA CCTGCTTAAA CCCACTGATC AGGTTTGCTC AACACCTCCT 
GCCTCTGAAA AACCAGAACT TATTGAATCC CCAGAACCTG CACCGGTCAC ACCTCAGAGC 
TCCATCGTGA GGTCAGAGGT TATGACCTCA AACCCTGTGG TGATCCACCA TGCTCAGGCC 
TCTCCAGTAG CATCACAACA ATCCCAGCAA TCAACAGCAC CTTCACCCAT CCCTACACAA 
AGAAGAGAGT CTACACCGTC ACCCACTAAA CAGCTCAGAA AACCCTCACT GGAACAAACA 
CAAAACAACA CCAGTAATGG AGTTTCTGCT AATGGCGTAC ATGGCAAGGG TCCTTCTCCA 
AACCTGATCA TTGAGGAGAT CCACGCCAGC CACAGCAAGA ACAAGAACAG AGCTGTGTCC 
ATAGAGGCTG CAGAGAAGGA ATGGGAGGAG AAAAGGCAGA ACTTGGGTCA TTATGATGGA 
CAGGAGTTTG AGCGGATTCT CCAGGAAGCA GAAGCAAACA TGCTGAGGGG AATACCAAAC 
CTCGAGGTGC CCAGTCAACT TGAGGGTTCT CCTGCTCCAC CTGCCATGCT TCCTGCCTCT 
CTGGAGGAGG TAGTAGAAAT AAACGAACCA GTAGCAGCAC CAAGAGAACA CGATGACAAT 
CAGCCAAACT CTGAGAAACC AGCTAAGACT GCTTCAGAGA AACCTCCCAA ACCTCTGGAG 
AAACCAGTTA AGCCAGCCTT GGAGAAACCT CTTAAACCCA GCCTATCCAT CAAATCCAAC 
CTAGAGAGAC AGAGTAAGAC TCAGGAAAAA GTTAAACTTC AGAGCACAGA GAAAACCAAC 
AAGTCTCCTC CTCCACCCCC ACCTCGACGC AACTACACCA CCAACACCGG CATGACCACC 
ACCCGCTCTG GAGAGGTCAT TTACACCAGC AGGAAAGAGT CTGTAACGGA AGGTGAAGAG 
GAGTCCAACA TTTCAACAAT GTCTCAGCCT GAACCAAAGA CCCCCCCAGA GGTAAAGCCC 
AAACCCATCA CCCCTCCACC TATCTCTGCC TCAGCCATCA CTGAAGAAGA GGACGAAGGA 
GACAAGATTA TGGCAGAGCT ACAGGTTTTC CAGAAGTGCT CTGTTAAGGA GGTAGGGCTC 
AAGGGTTTGG TAGAGCCACA GATCAGAGAG CTAAGACCTG GGGTCTTACT GCCCCTTAAA 
GATAAGAAGA ACACAGAGAA CCAAGATGAC AAGAATACTG ACACAGATGA GAATGGCAAC 
AATACTATTC GTCAAAGTCC TGGGGTCATC TACTATGTCA CAGCTCAGAT CTCCAAGGGA 
ACTCCACCAC CGTCAGAGGA ATCAACTGAG CGTAGAGATG CATCGCAGTC TTTCCCATCA 
CAGGTGTCAC AAATCAATGC TTCTGACCAA TCCCAAAGCC AGCAGCAGGT TTCCAATAAC 
AAATCAGATG CCCTCAAAAA CATTGGCCTA GCCAACTTTT CTGGCACCCA GCAACAAAAT 
CAGCTGTCTC GTTCAGCCTC ACTAAAGTTA CATCCAGCCT CTTCATGGGA AGAAGTTCCT 
GGTAGCCCAA CACAAGGGAA GGTTCATGTG GTCAAGGCTG CTCAAGTGCA GGTTAGCATA 
GAGGAGAGGG TAGAGTCTCC AACAGCTGTT TGCTCACCAG TCTCTTTTGG AGACTCTGAA 
CCCATCCTTT CTAAAGTGAT CCCTGTCGAG ACAGCTTTGA CAAATCAAAA GCTTCCATTT 
GGTTCATCCA AGGTGAAGGA AAGGAAATTT GAGCAAATGG AGGAAAATAA TGAGGCTTTT 
CTCAGTCCCG ATTTGTCAGG AGAAGAGCCA CCTCCTCCAC CACCTGATAA CTTTGCCTTT 
ATGATAACCA ACACCAAGGT TCAAGCCCTC TCCACTGGAG AGTACCATCA GCTTGTCAAC 
GCCAAGAAAG GAAGTGTACA GACAGTGACT TTTGGCAACA AACAACCAAG TGCTTCTTAC 
AGTGGCATGG CATCAGGAGG TGATGCTAAT ACCAATGGCC CTCCTGGAGC TGATAACACT 
AGTAAAAAGC CAGTTATCAT AATCTTTGAT GAGCCAATGG ACATCCGTTC AGCTTACAAG 
CGCCTCTCTA CCGTTTTCGA ATGTGAGGAG GAGCTAGATC GGATGCTTGC AGAAGAAAGA 
ATAGATGAGG AGAGTGAGGA AACTGAGGAA GAAGAATGGA CGAGAGGAGA GAAGGTCAAA 
CAGAATGGAG TTGAAAGACA GTCTGTAAAA AGAACTCTTG CAGATGTCAC AGATGGTGAA 
GTCAAGAACT CTTCATCATC CTTACTGGTT GCTGGTGATG CAAAGCAAGA TGGGAAGAAA 
AAGTTTAAGT TGAAATTTCC CAAGAAACAG CTAGCAGCAT TAACACAAGC TATTCGGGCT 
GGAAGTAAAA CAGGCAAAAA GACTTTGCAA GTTGTCGTCT ATGAGGATGA GGAAGAGTCC 
GATGGCACTG TCAAGCAACA TAAAGAGACC AAGAGGTATG AAATTGCTAG CTCCAAAACA 
GAATCCTCAA AATCAAATAC ACAGGAATCT AATGGACGAA CTGATGAGAT TCGTAAGAGC 
ACCTACAAAA CACTTGACAG TCTAGAGCAA ACAATAAAGC AGCTAGAGAA TACAATTTGC 
GAAATGGGAC CAACGTCTTC GGAAGACACT GAAAGCCTAA CGAATTCTGA AACTCAAGAA 
AACAAAGTAG TGGAGAATTC AGGCCACTCA GAGAAGGGAC TTTCTTCCAA AACCTTGGTT 
CCTAGGTTGT CTGCTGCCAG TAAGGGTCCT AGTCTCCGAA AGAAGACCAA ACCACAGCTC 
CTTCCTCGGC CTACCAACAT ACCAACTACA GGGGTCTCAG TAACACCAGT CGCTACCCAC 
CAGAACGCCA ACGTGGTCTC ACCTACTAGT CGGATGCCAG TGCCAGTTTC TGTCAAGGCG 
CGGCAGCCAT CAAGCACCTC AGAAAAAGAG AGGGCAACAA AACCGCAAAA ACTACAGGAC 
TCTCAGAGGC AGTTCCGCCA GGCTAATGGA AGTGCTAAGA GATCTGGAGG AGATCCCAAA 
TCCACTTCTC CTACCGTGCC TGCTTCTAAA ATTCCCGCTT TTTCCACTAG TACGGGAAAA 
AGCTTGTCCC AGTCTGATTC TACTAATAAT GTTAATTCTT CTTCTCCTTT ATCTTCTTCC 
CTTCCCACCA GCAAATCCTC TATCCCTCCT CCTCGATCCA GTTCCATCCC CTCCTCCCAC 
ATCCCCTCTA TTTCTAACGG TTCTTTAAAA TATGCCCCTC CCACTCACAG CACTAAAGGC 
CTCTCTATTC CCACACAGAC ACAAAATGGT CGACCTTCCA CTTCTTCTTC CTCCTCCTGT 
TCCCACTCCC CCTTGTCCCC GACTATGTTG AGTCAGAGTG CGAAGAGTAT CCGTACCATT 
CACACACCCA GCTTCAACAG CTACAGTCGG CCGCACAACG GAAGTGCCGG TAAATCAGCT 
ATACCAACGG TAACTGTCGC TAAGGATGCT GCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_005162585.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162642.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X2, mRNA.

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CloneID	ODa28373
Clone ID Related Accession (Same CDS sequence)	XM_005162585.4 , XM_005162585.3
Accession Version	XM_005162585.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	sickle tail protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162585.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC ACAGAGAACC AAGATGACAA GAATACTGAC 
ACAGATGAGA ATGGCAACAA TACTATTCGT CAAAGTCCTG GGGTCATCTA CTATGTCACA 
GCTCAGATCT CCAAGGGAAC TCCACCACCG TCAGAGGAAT CAACTGAGCG TAGAGATGCA 
TCGCAGTCTT TCCCATCACA GGTGTCACAA ATCAATGCTT CTGACCAATC CCAAAGCCAG 
CAGCAGGTTT CCAATAACAA ATCAGATGCC CTCAAAAACA TTGGCCTAGC CAACTTTTCT 
GGCACCCAGC AACAAAATCA GCTGTCTCGT TCAGCCTCAC TAAAGTTACA TCCAGCCTCT 
TCATGGGAAG AAGTTCCTGG TAGCCCAACA CAAGGGAAGG TTCATGTGGT CAAGGCTGCT 
CAAGTGCAGG TTAGCATAGA GGAGAGGGTA GAGTCTCCAA CAGCTGTTTG CTCACCAGTC 
TCTTTTGGAG ACTCTGAACC CATCCTTTCT AAAGTGATCC CTGTCGAGAC AGCTTTGACA 
AATCAAAAGC TTCCATTTGG TTCATCCAAG GTGAAGGAAA GGAAATTTGA GCAAATGGAG 
GAAAATAATG AGGCTTTTCT CAGTCCCGAT TTGTCAGGAG AAGAGCCACC TCCTCCACCA 
CCTGATAACT TTGCCTTTAT GATAACCAAC ACCAAGGTTC AAGCCCTCTC CACTGGAGAG 
TACCATCAGC TTGTCAACGC CAAGAAAGGA AGTGTACAGA CAGTGACTTT TGGCAACAAA 
CAACCAAGTG CTTCTTACAG TGGCATGGCA TCAGGAGGTG ATGCTAATAC CAATGGCCCT 
CCTGGAGCTG ATAACACTAG TAAAAAGCCA GTTATCATAA TCTTTGATGA GCCAATGGAC 
ATCCGTTCAG CTTACAAGCG CCTCTCTACC GTTTTCGAAT GTGAGGAGGA GCTAGATCGG 
ATGCTTGCAG AAGAAAGAAT AGATGAGGAG AGTGAGGAAA CTGAGGAAGA AGAATGGACG 
AGAGGAGAGA AGGTCAAACA GAATGGAGTT GAAAGACAGT CTGTAAAAAG AACTCTTGCA 
GATGTCACAG ATGGTGAAGT CAAGAACTCT TCATCATCCT TACTGGTTGC TGGTGATGCA 
AAGCAAGATG GGAAGAAAAA GTTTAAGTTG AAATTTCCCA AGAAACAGCT AGCAGCATTA 
ACACAAGCTA TTCGGGCTGG AAGTAAAACA GGCAAAAAGA CTTTGCAAGT TGTCGTCTAT 
GAGGATGAGG AAGAGTCCGA TGGCACTGTC AAGCAACATA AAGAGACCAA GAGGTATGAA 
ATTGCTAGCT CCAAAACAGA ATCCTCAAAA TCAAATACAC AGGAATCTAA TGGACGAACT 
GATGAGATTC GTAAGAGCAC CTACAAAACA CTTGACAGTC TAGAGCAAAC AATAAAGCAG 
CTAGAGAATA CAATTTGCGA AATGGGACCA ACGTCTTCGG AAGACACTGA AAGCCTAACG 
AATTCTGAAA CTCAAGAAAA CAAAGTAGTG GAGAATTCAG GCCACTCAGA GAAGGGACTT 
TCTTCCAAAA CCTTGGTTCC TAGGTTGTCT GCTGCCAGTA AGGGTCCTAG TCTCCGAAAG 
AAGACCAAAC CACAGCTCCT TCCTCGGCCT ACCAACATAC CAACTACAGG GGTCTCAGTA 
ACACCAGTCG CTACCCACCA GAACGCCAAC GTGGTCTCAC CTACTAGTCG GATGCCAGTG 
CCAGTTTCTG TCAAGGCGCG GCAGCCATCA AGCACCTCAG AAAAAGAGAG GGCAACAAAA 
CCGCAAAAAC TACAGGACTC TCAGAGGCAG TTCCGCCAGG CTAATGGAAG TGCTAAGAGA 
TCTGGAGGAG ATCCCAAATC CACTTCTCCT ACCGTGCCTG CTTCTAAAAT TCCCGCTTTT 
TCCACTAGTA CGGGAAAAAG CTTGTCCCAG TCTGATTCTA CTAATAATGT TAATTCTTCT 
TCTCCTTTAT CTTCTTCCCT TCCCACCAGC AAATCCTCTA TCCCTCCTCC TCGATCCAGT 
TCCATCCCCT CCTCCCACAT CCCCTCTATT TCTAACGGTT CTTTAAAATA TGCCCCTCCC 
ACTCACAGCA CTAAAGGCCT CTCTATTCCC ACACAGACAC AAAATGGTCG ACCTTCCACT 
TCTTCTTCCT CCTCCTGTTC CCACTCCCCC TTGTCCCCGA CTATGTTGAG TCAGAGTGCG 
AAGAGTATCC GTACCATTCA CACACCCAGC TTCAACAGCT ACAGTCGGCC GCACAACGGA 
AGTGCCGGTA AATCAGCTAT ACCAACGGTA ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162642.1
CDS	596..6709
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_005162585.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162585.4

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC ACAGAGAACC AAGATGACAA GAATACTGAC 
ACAGATGAGA ATGGCAACAA TACTATTCGT CAAAGTCCTG GGGTCATCTA CTATGTCACA 
GCTCAGATCT CCAAGGGAAC TCCACCACCG TCAGAGGAAT CAACTGAGCG TAGAGATGCA 
TCGCAGTCTT TCCCATCACA GGTGTCACAA ATCAATGCTT CTGACCAATC CCAAAGCCAG 
CAGCAGGTTT CCAATAACAA ATCAGATGCC CTCAAAAACA TTGGCCTAGC CAACTTTTCT 
GGCACCCAGC AACAAAATCA GCTGTCTCGT TCAGCCTCAC TAAAGTTACA TCCAGCCTCT 
TCATGGGAAG AAGTTCCTGG TAGCCCAACA CAAGGGAAGG TTCATGTGGT CAAGGCTGCT 
CAAGTGCAGG TTAGCATAGA GGAGAGGGTA GAGTCTCCAA CAGCTGTTTG CTCACCAGTC 
TCTTTTGGAG ACTCTGAACC CATCCTTTCT AAAGTGATCC CTGTCGAGAC AGCTTTGACA 
AATCAAAAGC TTCCATTTGG TTCATCCAAG GTGAAGGAAA GGAAATTTGA GCAAATGGAG 
GAAAATAATG AGGCTTTTCT CAGTCCCGAT TTGTCAGGAG AAGAGCCACC TCCTCCACCA 
CCTGATAACT TTGCCTTTAT GATAACCAAC ACCAAGGTTC AAGCCCTCTC CACTGGAGAG 
TACCATCAGC TTGTCAACGC CAAGAAAGGA AGTGTACAGA CAGTGACTTT TGGCAACAAA 
CAACCAAGTG CTTCTTACAG TGGCATGGCA TCAGGAGGTG ATGCTAATAC CAATGGCCCT 
CCTGGAGCTG ATAACACTAG TAAAAAGCCA GTTATCATAA TCTTTGATGA GCCAATGGAC 
ATCCGTTCAG CTTACAAGCG CCTCTCTACC GTTTTCGAAT GTGAGGAGGA GCTAGATCGG 
ATGCTTGCAG AAGAAAGAAT AGATGAGGAG AGTGAGGAAA CTGAGGAAGA AGAATGGACG 
AGAGGAGAGA AGGTCAAACA GAATGGAGTT GAAAGACAGT CTGTAAAAAG AACTCTTGCA 
GATGTCACAG ATGGTGAAGT CAAGAACTCT TCATCATCCT TACTGGTTGC TGGTGATGCA 
AAGCAAGATG GGAAGAAAAA GTTTAAGTTG AAATTTCCCA AGAAACAGCT AGCAGCATTA 
ACACAAGCTA TTCGGGCTGG AAGTAAAACA GGCAAAAAGA CTTTGCAAGT TGTCGTCTAT 
GAGGATGAGG AAGAGTCCGA TGGCACTGTC AAGCAACATA AAGAGACCAA GAGGTATGAA 
ATTGCTAGCT CCAAAACAGA ATCCTCAAAA TCAAATACAC AGGAATCTAA TGGACGAACT 
GATGAGATTC GTAAGAGCAC CTACAAAACA CTTGACAGTC TAGAGCAAAC AATAAAGCAG 
CTAGAGAATA CAATTTGCGA AATGGGACCA ACGTCTTCGG AAGACACTGA AAGCCTAACG 
AATTCTGAAA CTCAAGAAAA CAAAGTAGTG GAGAATTCAG GCCACTCAGA GAAGGGACTT 
TCTTCCAAAA CCTTGGTTCC TAGGTTGTCT GCTGCCAGTA AGGGTCCTAG TCTCCGAAAG 
AAGACCAAAC CACAGCTCCT TCCTCGGCCT ACCAACATAC CAACTACAGG GGTCTCAGTA 
ACACCAGTCG CTACCCACCA GAACGCCAAC GTGGTCTCAC CTACTAGTCG GATGCCAGTG 
CCAGTTTCTG TCAAGGCGCG GCAGCCATCA AGCACCTCAG AAAAAGAGAG GGCAACAAAA 
CCGCAAAAAC TACAGGACTC TCAGAGGCAG TTCCGCCAGG CTAATGGAAG TGCTAAGAGA 
TCTGGAGGAG ATCCCAAATC CACTTCTCCT ACCGTGCCTG CTTCTAAAAT TCCCGCTTTT 
TCCACTAGTA CGGGAAAAAG CTTGTCCCAG TCTGATTCTA CTAATAATGT TAATTCTTCT 
TCTCCTTTAT CTTCTTCCCT TCCCACCAGC AAATCCTCTA TCCCTCCTCC TCGATCCAGT 
TCCATCCCCT CCTCCCACAT CCCCTCTATT TCTAACGGTT CTTTAAAATA TGCCCCTCCC 
ACTCACAGCA CTAAAGGCCT CTCTATTCCC ACACAGACAC AAAATGGTCG ACCTTCCACT 
TCTTCTTCCT CCTCCTGTTC CCACTCCCCC TTGTCCCCGA CTATGTTGAG TCAGAGTGCG 
AAGAGTATCC GTACCATTCA CACACCCAGC TTCAACAGCT ACAGTCGGCC GCACAACGGA 
AGTGCCGGTA AATCAGCTAT ACCAACGGTA ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_005162585.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162642.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X1, mRNA.

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CloneID	ODa28373
Clone ID Related Accession (Same CDS sequence)	XM_005162585.4 , XM_005162585.3
Accession Version	XM_005162585.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6114bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC ACAGAGAACC AAGATGACAA GAATACTGAC 
ACAGATGAGA ATGGCAACAA TACTATTCGT CAAAGTCCTG GGGTCATCTA CTATGTCACA 
GCTCAGATCT CCAAGGGAAC TCCACCACCG TCAGAGGAAT CAACTGAGCG TAGAGATGCA 
TCGCAGTCTT TCCCATCACA GGTGTCACAA ATCAATGCTT CTGACCAATC CCAAAGCCAG 
CAGCAGGTTT CCAATAACAA ATCAGATGCC CTCAAAAACA TTGGCCTAGC CAACTTTTCT 
GGCACCCAGC AACAAAATCA GCTGTCTCGT TCAGCCTCAC TAAAGTTACA TCCAGCCTCT 
TCATGGGAAG AAGTTCCTGG TAGCCCAACA CAAGGGAAGG TTCATGTGGT CAAGGCTGCT 
CAAGTGCAGG TTAGCATAGA GGAGAGGGTA GAGTCTCCAA CAGCTGTTTG CTCACCAGTC 
TCTTTTGGAG ACTCTGAACC CATCCTTTCT AAAGTGATCC CTGTCGAGAC AGCTTTGACA 
AATCAAAAGC TTCCATTTGG TTCATCCAAG GTGAAGGAAA GGAAATTTGA GCAAATGGAG 
GAAAATAATG AGGCTTTTCT CAGTCCCGAT TTGTCAGGAG AAGAGCCACC TCCTCCACCA 
CCTGATAACT TTGCCTTTAT GATAACCAAC ACCAAGGTTC AAGCCCTCTC CACTGGAGAG 
TACCATCAGC TTGTCAACGC CAAGAAAGGA AGTGTACAGA CAGTGACTTT TGGCAACAAA 
CAACCAAGTG CTTCTTACAG TGGCATGGCA TCAGGAGGTG ATGCTAATAC CAATGGCCCT 
CCTGGAGCTG ATAACACTAG TAAAAAGCCA GTTATCATAA TCTTTGATGA GCCAATGGAC 
ATCCGTTCAG CTTACAAGCG CCTCTCTACC GTTTTCGAAT GTGAGGAGGA GCTAGATCGG 
ATGCTTGCAG AAGAAAGAAT AGATGAGGAG AGTGAGGAAA CTGAGGAAGA AGAATGGACG 
AGAGGAGAGA AGGTCAAACA GAATGGAGTT GAAAGACAGT CTGTAAAAAG AACTCTTGCA 
GATGTCACAG ATGGTGAAGT CAAGAACTCT TCATCATCCT TACTGGTTGC TGGTGATGCA 
AAGCAAGATG GGAAGAAAAA GTTTAAGTTG AAATTTCCCA AGAAACAGCT AGCAGCATTA 
ACACAAGCTA TTCGGGCTGG AAGTAAAACA GGCAAAAAGA CTTTGCAAGT TGTCGTCTAT 
GAGGATGAGG AAGAGTCCGA TGGCACTGTC AAGCAACATA AAGAGACCAA GAGGTATGAA 
ATTGCTAGCT CCAAAACAGA ATCCTCAAAA TCAAATACAC AGGAATCTAA TGGACGAACT 
GATGAGATTC GTAAGAGCAC CTACAAAACA CTTGACAGTC TAGAGCAAAC AATAAAGCAG 
CTAGAGAATA CAATTTGCGA AATGGGACCA ACGTCTTCGG AAGACACTGA AAGCCTAACG 
AATTCTGAAA CTCAAGAAAA CAAAGTAGTG GAGAATTCAG GCCACTCAGA GAAGGGACTT 
TCTTCCAAAA CCTTGGTTCC TAGGTTGTCT GCTGCCAGTA AGGGTCCTAG TCTCCGAAAG 
AAGACCAAAC CACAGCTCCT TCCTCGGCCT ACCAACATAC CAACTACAGG GGTCTCAGTA 
ACACCAGTCG CTACCCACCA GAACGCCAAC GTGGTCTCAC CTACTAGTCG GATGCCAGTG 
CCAGTTTCTG TCAAGGCGCG GCAGCCATCA AGCACCTCAG AAAAAGAGAG GGCAACAAAA 
CCGCAAAAAC TACAGGACTC TCAGAGGCAG TTCCGCCAGG CTAATGGAAG TGCTAAGAGA 
TCTGGAGGAG ATCCCAAATC CACTTCTCCT ACCGTGCCTG CTTCTAAAAT TCCCGCTTTT 
TCCACTAGTA CGGGAAAAAG CTTGTCCCAG TCTGATTCTA CTAATAATGT TAATTCTTCT 
TCTCCTTTAT CTTCTTCCCT TCCCACCAGC AAATCCTCTA TCCCTCCTCC TCGATCCAGT 
TCCATCCCCT CCTCCCACAT CCCCTCTATT TCTAACGGTT CTTTAAAATA TGCCCCTCCC 
ACTCACAGCA CTAAAGGCCT CTCTATTCCC ACACAGACAC AAAATGGTCG ACCTTCCACT 
TCTTCTTCCT CCTCCTGTTC CCACTCCCCC TTGTCCCCGA CTATGTTGAG TCAGAGTGCG 
AAGAGTATCC GTACCATTCA CACACCCAGC TTCAACAGCT ACAGTCGGCC GCACAACGGA 
AGTGCCGGTA AATCAGCTAT ACCAACGGTA ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162642.1
CDS	615..6728
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_005162585.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162585.3

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGGTTTTCCA GAAGTGCTCT 
GTTAAGGAGG TAGGGCTCAA GGGTTTGGTA GAGCCACAGA TCAGAGAGCT AAGACCTGGG 
GTCTTACTGC CCCTTAAAGA TAAGAAGAAC ACAGAGAACC AAGATGACAA GAATACTGAC 
ACAGATGAGA ATGGCAACAA TACTATTCGT CAAAGTCCTG GGGTCATCTA CTATGTCACA 
GCTCAGATCT CCAAGGGAAC TCCACCACCG TCAGAGGAAT CAACTGAGCG TAGAGATGCA 
TCGCAGTCTT TCCCATCACA GGTGTCACAA ATCAATGCTT CTGACCAATC CCAAAGCCAG 
CAGCAGGTTT CCAATAACAA ATCAGATGCC CTCAAAAACA TTGGCCTAGC CAACTTTTCT 
GGCACCCAGC AACAAAATCA GCTGTCTCGT TCAGCCTCAC TAAAGTTACA TCCAGCCTCT 
TCATGGGAAG AAGTTCCTGG TAGCCCAACA CAAGGGAAGG TTCATGTGGT CAAGGCTGCT 
CAAGTGCAGG TTAGCATAGA GGAGAGGGTA GAGTCTCCAA CAGCTGTTTG CTCACCAGTC 
TCTTTTGGAG ACTCTGAACC CATCCTTTCT AAAGTGATCC CTGTCGAGAC AGCTTTGACA 
AATCAAAAGC TTCCATTTGG TTCATCCAAG GTGAAGGAAA GGAAATTTGA GCAAATGGAG 
GAAAATAATG AGGCTTTTCT CAGTCCCGAT TTGTCAGGAG AAGAGCCACC TCCTCCACCA 
CCTGATAACT TTGCCTTTAT GATAACCAAC ACCAAGGTTC AAGCCCTCTC CACTGGAGAG 
TACCATCAGC TTGTCAACGC CAAGAAAGGA AGTGTACAGA CAGTGACTTT TGGCAACAAA 
CAACCAAGTG CTTCTTACAG TGGCATGGCA TCAGGAGGTG ATGCTAATAC CAATGGCCCT 
CCTGGAGCTG ATAACACTAG TAAAAAGCCA GTTATCATAA TCTTTGATGA GCCAATGGAC 
ATCCGTTCAG CTTACAAGCG CCTCTCTACC GTTTTCGAAT GTGAGGAGGA GCTAGATCGG 
ATGCTTGCAG AAGAAAGAAT AGATGAGGAG AGTGAGGAAA CTGAGGAAGA AGAATGGACG 
AGAGGAGAGA AGGTCAAACA GAATGGAGTT GAAAGACAGT CTGTAAAAAG AACTCTTGCA 
GATGTCACAG ATGGTGAAGT CAAGAACTCT TCATCATCCT TACTGGTTGC TGGTGATGCA 
AAGCAAGATG GGAAGAAAAA GTTTAAGTTG AAATTTCCCA AGAAACAGCT AGCAGCATTA 
ACACAAGCTA TTCGGGCTGG AAGTAAAACA GGCAAAAAGA CTTTGCAAGT TGTCGTCTAT 
GAGGATGAGG AAGAGTCCGA TGGCACTGTC AAGCAACATA AAGAGACCAA GAGGTATGAA 
ATTGCTAGCT CCAAAACAGA ATCCTCAAAA TCAAATACAC AGGAATCTAA TGGACGAACT 
GATGAGATTC GTAAGAGCAC CTACAAAACA CTTGACAGTC TAGAGCAAAC AATAAAGCAG 
CTAGAGAATA CAATTTGCGA AATGGGACCA ACGTCTTCGG AAGACACTGA AAGCCTAACG 
AATTCTGAAA CTCAAGAAAA CAAAGTAGTG GAGAATTCAG GCCACTCAGA GAAGGGACTT 
TCTTCCAAAA CCTTGGTTCC TAGGTTGTCT GCTGCCAGTA AGGGTCCTAG TCTCCGAAAG 
AAGACCAAAC CACAGCTCCT TCCTCGGCCT ACCAACATAC CAACTACAGG GGTCTCAGTA 
ACACCAGTCG CTACCCACCA GAACGCCAAC GTGGTCTCAC CTACTAGTCG GATGCCAGTG 
CCAGTTTCTG TCAAGGCGCG GCAGCCATCA AGCACCTCAG AAAAAGAGAG GGCAACAAAA 
CCGCAAAAAC TACAGGACTC TCAGAGGCAG TTCCGCCAGG CTAATGGAAG TGCTAAGAGA 
TCTGGAGGAG ATCCCAAATC CACTTCTCCT ACCGTGCCTG CTTCTAAAAT TCCCGCTTTT 
TCCACTAGTA CGGGAAAAAG CTTGTCCCAG TCTGATTCTA CTAATAATGT TAATTCTTCT 
TCTCCTTTAT CTTCTTCCCT TCCCACCAGC AAATCCTCTA TCCCTCCTCC TCGATCCAGT 
TCCATCCCCT CCTCCCACAT CCCCTCTATT TCTAACGGTT CTTTAAAATA TGCCCCTCCC 
ACTCACAGCA CTAAAGGCCT CTCTATTCCC ACACAGACAC AAAATGGTCG ACCTTCCACT 
TCTTCTTCCT CCTCCTGTTC CCACTCCCCC TTGTCCCCGA CTATGTTGAG TCAGAGTGCG 
AAGAGTATCC GTACCATTCA CACACCCAGC TTCAACAGCT ACAGTCGGCC GCACAACGGA 
AGTGCCGGTA AATCAGCTAT ACCAACGGTA ACTGTCGCTA AGGATGCTGC GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_005162587.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162644.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X2, mRNA.

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CloneID	ODa28375
Clone ID Related Accession (Same CDS sequence)	XM_005162587.3
Accession Version	XM_005162587.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGAACACAGA GAACCAAGAT 
GACAAGAATA CTGACACAGA TGAGAATGGC AACAATACTA TTCGTCAAAG TCCTGGGGTC 
ATCTACTATG TCACAGCTCA GATCTCCAAG GGAACTCCAC CACCGTCAGA GGAATCAACT 
GAGCGTAGAG ATGCATCGCA GTCTTTCCCA TCACAGGTGT CACAAATCAA TGCTTCTGAC 
CAATCCCAAA GCCAGCAGCA GGTTTCCAAT AACAAATCAG ATGCCCTCAA AAACATTGGC 
CTAGCCAACT TTTCTGGCAC CCAGCAACAA AATCAGCTGT CTCGTTCAGC CTCACTAAAG 
TTACATCCAG CCTCTTCATG GGAAGAAGTT CCTGGTAGCC CAACACAAGG GAAGGTTCAT 
GTGGTCAAGG CTGCTCAAGT GCAGGTTAGC ATAGAGGAGA GGGTAGAGTC TCCAACAGCT 
GTTTGCTCAC CAGTCTCTTT TGGAGACTCT GAACCCATCC TTTCTAAAGT GATCCCTGTC 
GAGACAGCTT TGACAAATCA AAAGCTTCCA TTTGGTTCAT CCAAGGTGAA GGAAAGGAAA 
TTTGAGCAAA TGGAGGAAAA TAATGAGGCT TTTCTCAGTC CCGATTTGTC AGGAGAAGAG 
CCACCTCCTC CACCACCTGA TAACTTTGCC TTTATGATAA CCAACACCAA GGTTCAAGCC 
CTCTCCACTG GAGAGTACCA TCAGCTTGTC AACGCCAAGA AAGGAAGTGT ACAGACAGTG 
ACTTTTGGCA ACAAACAACC AAGTGCTTCT TACAGTGGCA TGGCATCAGG AGGTGATGCT 
AATACCAATG GCCCTCCTGG AGCTGATAAC ACTAGTAAAA AGCCAGTTAT CATAATCTTT 
GATGAGCCAA TGGACATCCG TTCAGCTTAC AAGCGCCTCT CTACCGTTTT CGAATGTGAG 
GAGGAGCTAG ATCGGATGCT TGCAGAAGAA AGAATAGATG AGGAGAGTGA GGAAACTGAG 
GAAGAAGAAT GGACGAGAGG AGAGAAGGTC AAACAGAATG GAGTTGAAAG ACAGTCTGTA 
AAAAGAACTC TTGCAGATGT CACAGATGGT GAAGTCAAGA ACTCTTCATC ATCCTTACTG 
GTTGCTGGTG ATGCAAAGCA AGATGGGAAG AAAAAGTTTA AGTTGAAATT TCCCAAGAAA 
CAGCTAGCAG CATTAACACA AGCTATTCGG GCTGGAAGTA AAACAGGCAA AAAGACTTTG 
CAAGTTGTCG TCTATGAGGA TGAGGAAGAG TCCGATGGCA CTGTCAAGCA ACATAAAGAG 
ACCAAGAGGT ATGAAATTGC TAGCTCCAAA ACAGAATCCT CAAAATCAAA TACACAGGAA 
TCTAATGGAC GAACTGATGA GATTCGTAAG AGCACCTACA AAACACTTGA CAGTCTAGAG 
CAAACAATAA AGCAGCTAGA GAATACAATT TGCGAAATGG GACCAACGTC TTCGGAAGAC 
ACTGAAAGCC TAACGAATTC TGAAACTCAA GAAAACAAAG TAGTGGAGAA TTCAGGCCAC 
TCAGAGAAGG GACTTTCTTC CAAAACCTTG GTTCCTAGGT TGTCTGCTGC CAGTAAGGGT 
CCTAGTCTCC GAAAGAAGAC CAAACCACAG CTCCTTCCTC GGCCTACCAA CATACCAACT 
ACAGGGGTCT CAGTAACACC AGTCGCTACC CACCAGAACG CCAACGTGGT CTCACCTACT 
AGTCGGATGC CAGTGCCAGT TTCTGTCAAG GCGCGGCAGC CATCAAGCAC CTCAGAAAAA 
GAGAGGGCAA CAAAACCGCA AAAACTACAG GACTCTCAGA GGCAGTTCCG CCAGGCTAAT 
GGAAGTGCTA AGAGATCTGG AGGAGATCCC AAATCCACTT CTCCTACCGT GCCTGCTTCT 
AAAATTCCCG CTTTTTCCAC TAGTACGGGA AAAAGCTTGT CCCAGTCTGA TTCTACTAAT 
AATGTTAATT CTTCTTCTCC TTTATCTTCT TCCCTTCCCA CCAGCAAATC CTCTATCCCT 
CCTCCTCGAT CCAGTTCCAT CCCCTCCTCC CACATCCCCT CTATTTCTAA CGGTTCTTTA 
AAATATGCCC CTCCCACTCA CAGCACTAAA GGCCTCTCTA TTCCCACACA GACACAAAAT 
GGTCGACCTT CCACTTCTTC TTCCTCCTCC TGTTCCCACT CCCCCTTGTC CCCGACTATG 
TTGAGTCAGA GTGCGAAGAG TATCCGTACC ATTCACACAC CCAGCTTCAA CAGCTACAGT 
CGGCCGCACA ACGGAAGTGC CGGTAAATCA GCTATACCAA CGGTAACTGT CGCTAAGGAT 
GCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162644.1
CDS	615..6623
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERMKAMEEQIASLTGLVQHALLKSNSTNSNKEHSSERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_005162587.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162587.3

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGAGAGAGA ATGAAGGCCA TGGAGGAGCA AATAGCCAGT 
CTGACTGGTC TTGTGCAGCA CGCTCTCTTA AAGAGTAACA GCACTAACAG CAACAAGGAG 
CACTCCAGCG AAAGACCAGT GAAGTCTGGA TCACCAGCTC ACAGCACATC TAGCACAGGA 
GGCTCTCCAG TATTAGCACC AAAGGTTGTT GCTGCTCCTC CTGAGCGTAG CCCCGCCCCC 
ACACCACCTT TAGGACCTGC CCCTTTGCAT GTTAACCTTC ATCTGTTCAG GAAGAATGTC 
TCTGAGCTCC GCCTCCAACT ACAACATATG AGACAACTAC AGCTTCAGAA TCAGGAGTGT 
GTACGTGCTC AGATAAAGCG CGCAGAACAG GAGATCGGTG TGAAACTGGC TGAAATGCTG 
AGACGACAGG ATGATCCGAC ACAGAAACAG AGGATGCTTG TGGAGGAAGA GAGACACCGA 
TATCTCAGCA TGCAGGAAAG AGTTCTCACA CAGCTGGGGG ATCTGGAGCA GTTTGTGGAG 
ACTCTGAGGA CTGATTCGGG TCCTGCTGCG TCTCAGAGAG CAGTGACGCT GAAGGAGGTT 
GAGGATGGGG CTGTCAGCCT GAGGAAGGTG GGAGAAAACC TGGCTGGACT CAAAGGTGAG 
TTTCCGGCTC TTCAGTCCCG CATGCGCTCA GTGCTCAGGG TTGAAGTAGA GGCTGTAAAG 
TTCCTCAAAG AAGAGCCACA CAAACTGGAC AGCATGTTAA AGAGAGTCCG GACCCTCACT 
GACACACTCA GCAACCTTCG CAGGTGTGCC ACCGATAACC TGCTTAAACC CACTGATCAG 
GTTTGCTCAA CACCTCCTGC CTCTGAAAAA CCAGAACTTA TTGAATCCCC AGAACCTGCA 
CCGGTCACAC CTCAGAGCTC CATCGTGAGG TCAGAGGTTA TGACCTCAAA CCCTGTGGTG 
ATCCACCATG CTCAGGCCTC TCCAGTAGCA TCACAACAAT CCCAGCAATC AACAGCACCT 
TCACCCATCC CTACACAAAG AAGAGAGTCT ACACCGTCAC CCACTAAACA GCTCAGAAAA 
CCCTCACTGG AACAAACACA AAACAACACC AGTAATGGAG TTTCTGCTAA TGGCGTACAT 
GGCAAGGGTC CTTCTCCAAA CCTGATCATT GAGGAGATCC ACGCCAGCCA CAGCAAGAAC 
AAGAACAGAG CTGTGTCCAT AGAGGCTGCA GAGAAGGAAT GGGAGGAGAA AAGGCAGAAC 
TTGGGTCATT ATGATGGACA GGAGTTTGAG CGGATTCTCC AGGAAGCAGA AGCAAACATG 
CTGAGGGGAA TACCAAACCT CGAGGTGCCC AGTCAACTTG AGGGTTCTCC TGCTCCACCT 
GCCATGCTTC CTGCCTCTCT GGAGGAGGTA GTAGAAATAA ACGAACCAGT AGCAGCACCA 
AGAGAACACG ATGACAATCA GCCAAACTCT GAGAAACCAG CTAAGACTGC TTCAGAGAAA 
CCTCCCAAAC CTCTGGAGAA ACCAGTTAAG CCAGCCTTGG AGAAACCTCT TAAACCCAGC 
CTATCCATCA AATCCAACCT AGAGAGACAG AGTAAGACTC AGGAAAAAGT TAAACTTCAG 
AGCACAGAGA AAACCAACAA GTCTCCTCCT CCACCCCCAC CTCGACGCAA CTACACCACC 
AACACCGGCA TGACCACCAC CCGCTCTGGA GAGGTCATTT ACACCAGCAG GAAAGAGTCT 
GTAACGGAAG GTGAAGAGGA GTCCAACATT TCAACAATGT CTCAGCCTGA ACCAAAGACC 
CCCCCAGAGG TAAAGCCCAA ACCCATCACC CCTCCACCTA TCTCTGCCTC AGCCATCACT 
GAAGAAGAGG ACGAAGGAGA CAAGATTATG GCAGAGCTAC AGAACACAGA GAACCAAGAT 
GACAAGAATA CTGACACAGA TGAGAATGGC AACAATACTA TTCGTCAAAG TCCTGGGGTC 
ATCTACTATG TCACAGCTCA GATCTCCAAG GGAACTCCAC CACCGTCAGA GGAATCAACT 
GAGCGTAGAG ATGCATCGCA GTCTTTCCCA TCACAGGTGT CACAAATCAA TGCTTCTGAC 
CAATCCCAAA GCCAGCAGCA GGTTTCCAAT AACAAATCAG ATGCCCTCAA AAACATTGGC 
CTAGCCAACT TTTCTGGCAC CCAGCAACAA AATCAGCTGT CTCGTTCAGC CTCACTAAAG 
TTACATCCAG CCTCTTCATG GGAAGAAGTT CCTGGTAGCC CAACACAAGG GAAGGTTCAT 
GTGGTCAAGG CTGCTCAAGT GCAGGTTAGC ATAGAGGAGA GGGTAGAGTC TCCAACAGCT 
GTTTGCTCAC CAGTCTCTTT TGGAGACTCT GAACCCATCC TTTCTAAAGT GATCCCTGTC 
GAGACAGCTT TGACAAATCA AAAGCTTCCA TTTGGTTCAT CCAAGGTGAA GGAAAGGAAA 
TTTGAGCAAA TGGAGGAAAA TAATGAGGCT TTTCTCAGTC CCGATTTGTC AGGAGAAGAG 
CCACCTCCTC CACCACCTGA TAACTTTGCC TTTATGATAA CCAACACCAA GGTTCAAGCC 
CTCTCCACTG GAGAGTACCA TCAGCTTGTC AACGCCAAGA AAGGAAGTGT ACAGACAGTG 
ACTTTTGGCA ACAAACAACC AAGTGCTTCT TACAGTGGCA TGGCATCAGG AGGTGATGCT 
AATACCAATG GCCCTCCTGG AGCTGATAAC ACTAGTAAAA AGCCAGTTAT CATAATCTTT 
GATGAGCCAA TGGACATCCG TTCAGCTTAC AAGCGCCTCT CTACCGTTTT CGAATGTGAG 
GAGGAGCTAG ATCGGATGCT TGCAGAAGAA AGAATAGATG AGGAGAGTGA GGAAACTGAG 
GAAGAAGAAT GGACGAGAGG AGAGAAGGTC AAACAGAATG GAGTTGAAAG ACAGTCTGTA 
AAAAGAACTC TTGCAGATGT CACAGATGGT GAAGTCAAGA ACTCTTCATC ATCCTTACTG 
GTTGCTGGTG ATGCAAAGCA AGATGGGAAG AAAAAGTTTA AGTTGAAATT TCCCAAGAAA 
CAGCTAGCAG CATTAACACA AGCTATTCGG GCTGGAAGTA AAACAGGCAA AAAGACTTTG 
CAAGTTGTCG TCTATGAGGA TGAGGAAGAG TCCGATGGCA CTGTCAAGCA ACATAAAGAG 
ACCAAGAGGT ATGAAATTGC TAGCTCCAAA ACAGAATCCT CAAAATCAAA TACACAGGAA 
TCTAATGGAC GAACTGATGA GATTCGTAAG AGCACCTACA AAACACTTGA CAGTCTAGAG 
CAAACAATAA AGCAGCTAGA GAATACAATT TGCGAAATGG GACCAACGTC TTCGGAAGAC 
ACTGAAAGCC TAACGAATTC TGAAACTCAA GAAAACAAAG TAGTGGAGAA TTCAGGCCAC 
TCAGAGAAGG GACTTTCTTC CAAAACCTTG GTTCCTAGGT TGTCTGCTGC CAGTAAGGGT 
CCTAGTCTCC GAAAGAAGAC CAAACCACAG CTCCTTCCTC GGCCTACCAA CATACCAACT 
ACAGGGGTCT CAGTAACACC AGTCGCTACC CACCAGAACG CCAACGTGGT CTCACCTACT 
AGTCGGATGC CAGTGCCAGT TTCTGTCAAG GCGCGGCAGC CATCAAGCAC CTCAGAAAAA 
GAGAGGGCAA CAAAACCGCA AAAACTACAG GACTCTCAGA GGCAGTTCCG CCAGGCTAAT 
GGAAGTGCTA AGAGATCTGG AGGAGATCCC AAATCCACTT CTCCTACCGT GCCTGCTTCT 
AAAATTCCCG CTTTTTCCAC TAGTACGGGA AAAAGCTTGT CCCAGTCTGA TTCTACTAAT 
AATGTTAATT CTTCTTCTCC TTTATCTTCT TCCCTTCCCA CCAGCAAATC CTCTATCCCT 
CCTCCTCGAT CCAGTTCCAT CCCCTCCTCC CACATCCCCT CTATTTCTAA CGGTTCTTTA 
AAATATGCCC CTCCCACTCA CAGCACTAAA GGCCTCTCTA TTCCCACACA GACACAAAAT 
GGTCGACCTT CCACTTCTTC TTCCTCCTCC TGTTCCCACT CCCCCTTGTC CCCGACTATG 
TTGAGTCAGA GTGCGAAGAG TATCCGTACC ATTCACACAC CCAGCTTCAA CAGCTACAGT 
CGGCCGCACA ACGGAAGTGC CGGTAAATCA GCTATACCAA CGGTAACTGT CGCTAAGGAT 
GCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:ch211-285f17.1 ( XM_009297304.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295579.1 Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X3, mRNA.

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CloneID	ODa43820
Clone ID Related Accession (Same CDS sequence)	XM_009297304.2
Accession Version	XM_009297304.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	sickle tail protein homolog isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297304.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGCGAAAGA CCAGTGAAGT CTGGATCACC AGCTCACAGC 
ACATCTAGCA CAGGAGGCTC TCCAGTATTA GCACCAAAGG TTGTTGCTGC TCCTCCTGAG 
CGTAGCCCCG CCCCCACACC ACCTTTAGGA CCTGCCCCTT TGCATGTTAA CCTTCATCTG 
TTCAGGAAGA ATGTCTCTGA GCTCCGCCTC CAACTACAAC ATATGAGACA ACTACAGCTT 
CAGAATCAGG AGTGTGTACG TGCTCAGATA AAGCGCGCAG AACAGGAGAT CGGTGTGAAA 
CTGGCTGAAA TGCTGAGACG ACAGGATGAT CCGACACAGA AACAGAGGAT GCTTGTGGAG 
GAAGAGAGAC ACCGATATCT CAGCATGCAG GAAAGAGTTC TCACACAGCT GGGGGATCTG 
GAGCAGTTTG TGGAGACTCT GAGGACTGAT TCGGGTCCTG CTGCGTCTCA GAGAGCAGTG 
ACGCTGAAGG AGGTTGAGGA TGGGGCTGTC AGCCTGAGGA AGGTGGGAGA AAACCTGGCT 
GGACTCAAAG GTGAGTTTCC GGCTCTTCAG TCCCGCATGC GCTCAGTGCT CAGGGTTGAA 
GTAGAGGCTG TAAAGTTCCT CAAAGAAGAG CCACACAAAC TGGACAGCAT GTTAAAGAGA 
GTCCGGACCC TCACTGACAC ACTCAGCAAC CTTCGCAGGT GTGCCACCGA TAACCTGCTT 
AAACCCACTG ATCAGGTTTG CTCAACACCT CCTGCCTCTG AAAAACCAGA ACTTATTGAA 
TCCCCAGAAC CTGCACCGGT CACACCTCAG AGCTCCATCG TGAGGTCAGA GGTTATGACC 
TCAAACCCTG TGGTGATCCA CCATGCTCAG GCCTCTCCAG TAGCATCACA ACAATCCCAG 
CAATCAACAG CACCTTCACC CATCCCTACA CAAAGAAGAG AGTCTACACC GTCACCCACT 
AAACAGCTCA GAAAACCCTC ACTGGAACAA ACACAAAACA ACACCAGTAA TGGAGTTTCT 
GCTAATGGCG TACATGGCAA GGGTCCTTCT CCAAACCTGA TCATTGAGGA GATCCACGCC 
AGCCACAGCA AGAACAAGAA CAGAGCTGTG TCCATAGAGG CTGCAGAGAA GGAATGGGAG 
GAGAAAAGGC AGAACTTGGG TCATTATGAT GGACAGGAGT TTGAGCGGAT TCTCCAGGAA 
GCAGAAGCAA ACATGCTGAG GGGAATACCA AACCTCGAGG TGCCCAGTCA ACTTGAGGGT 
TCTCCTGCTC CACCTGCCAT GCTTCCTGCC TCTCTGGAGG AGGTAGTAGA AATAAACGAA 
CCAGTAGCAG CACCAAGAGA ACACGATGAC AATCAGCCAA ACTCTGAGAA ACCAGCTAAG 
ACTGCTTCAG AGAAACCTCC CAAACCTCTG GAGAAACCAG TTAAGCCAGC CTTGGAGAAA 
CCTCTTAAAC CCAGCCTATC CATCAAATCC AACCTAGAGA GACAGAGTAA GACTCAGGAA 
AAAGTTAAAC TTCAGAGCAC AGAGAAAACC AACAAGTCTC CTCCTCCACC CCCACCTCGA 
CGCAACTACA CCACCAACAC CGGCATGACC ACCACCCGCT CTGGAGAGGT CATTTACACC 
AGCAGGAAAG AGTCTGTAAC GGAAGGTGAA GAGGAGTCCA ACATTTCAAC AATGTCTCAG 
CCTGAACCAA AGACCCCCCC AGAGGTAAAG CCCAAACCCA TCACCCCTCC ACCTATCTCT 
GCCTCAGCCA TCACTGAAGA AGAGGACGAA GGAGACAAGA TTATGGCAGA GCTACAGGTT 
TTCCAGAAGT GCTCTGTTAA GGAGGTAGGG CTCAAGGGTT TGGTAGAGCC ACAGATCAGA 
GAGCTAAGAC CTGGGGTCTT ACTGCCCCTT AAAGATAAGA AGAACACAGA GAACCAAGAT 
GACAAGAATA CTGACACAGA TGAGAATGGC AACAATACTA TTCGTCAAAG TCCTGGGGTC 
ATCTACTATG TCACAGCTCA GATCTCCAAG GGAACTCCAC CACCGTCAGA GGAATCAACT 
GAGCGTAGAG ATGCATCGCA GTCTTTCCCA TCACAGGTGT CACAAATCAA TGCTTCTGAC 
CAATCCCAAA GCCAGCAGCA GGTTTCCAAT AACAAATCAG ATGCCCTCAA AAACATTGGC 
CTAGCCAACT TTTCTGGCAC CCAGCAACAA AATCAGCTGT CTCGTTCAGC CTCACTAAAG 
TTACATCCAG CCTCTTCATG GGAAGAAGTT CCTGGTAGCC CAACACAAGG GAAGGTTCAT 
GTGGTCAAGG CTGCTCAAGT GCAGGTTAGC ATAGAGGAGA GGGTAGAGTC TCCAACAGCT 
GTTTGCTCAC CAGTCTCTTT TGGAGACTCT GAACCCATCC TTTCTAAAGT GATCCCTGTC 
GAGACAGCTT TGACAAATCA AAAGCTTCCA TTTGGTTCAT CCAAGGTGAA GGAAAGGAAA 
TTTGAGCAAA TGGAGGAAAA TAATGAGGCT TTTCTCAGTC CCGATTTGTC AGGAGAAGAG 
CCACCTCCTC CACCACCTGA TAACTTTGCC TTTATGATAA CCAACACCAA GGTTCAAGCC 
CTCTCCACTG GAGAGTACCA TCAGCTTGTC AACGCCAAGA AAGGAAGTGT ACAGACAGTG 
ACTTTTGGCA ACAAACAACC AAGTGCTTCT TACAGTGGCA TGGCATCAGG AGGTGATGCT 
AATACCAATG GCCCTCCTGG AGCTGATAAC ACTAGTAAAA AGCCAGTTAT CATAATCTTT 
GATGAGCCAA TGGACATCCG TTCAGCTTAC AAGCGCCTCT CTACCGTTTT CGAATGTGAG 
GAGGAGCTAG ATCGGATGCT TGCAGAAGAA AGAATAGATG AGGAGAGTGA GGAAACTGAG 
GAAGAAGAAT GGACGAGAGG AGAGAAGGTC AAACAGAATG GAGTTGAAAG ACAGTCTGTA 
AAAAGAACTC TTGCAGATGT CACAGATGGT GAAGTCAAGA ACTCTTCATC ATCCTTACTG 
GTTGCTGGTG ATGCAAAGCA AGATGGGAAG AAAAAGTTTA AGTTGAAATT TCCCAAGAAA 
CAGCTAGCAG CATTAACACA AGCTATTCGG GCTGGAAGTA AAACAGGCAA AAAGACTTTG 
CAAGTTGTCG TCTATGAGGA TGAGGAAGAG TCCGATGGCA CTGTCAAGCA ACATAAAGAG 
ACCAAGAGGT ATGAAATTGC TAGCTCCAAA ACAGAATCCT CAAAATCAAA TACACAGGAA 
TCTAATGGAC GAACTGATGA GATTCGTAAG AGCACCTACA AAACACTTGA CAGTCTAGAG 
CAAACAATAA AGCAGCTAGA GAATACAATT TGCGAAATGG GACCAACGTC TTCGGAAGAC 
ACTGAAAGCC TAACGAATTC TGAAACTCAA GAAAACAAAG TAGTGGAGAA TTCAGGCCAC 
TCAGAGAAGG GACTTTCTTC CAAAACCTTG GTTCCTAGGT TGTCTGCTGC CAGTAAGGGT 
CCTAGTCTCC GAAAGAAGAC CAAACCACAG CTCCTTCCTC GGCCTACCAA CATACCAACT 
ACAGGGGTCT CAGTAACACC AGTCGCTACC CACCAGAACG CCAACGTGGT CTCACCTACT 
AGTCGGATGC CAGTGCCAGT TTCTGTCAAG GCGCGGCAGC CATCAAGCAC CTCAGAAAAA 
GAGAGGGCAA CAAAACCGCA AAAACTACAG GACTCTCAGA GGCAGTTCCG CCAGGCTAAT 
GGAAGTGCTA AGAGATCTGG AGGAGATCCC AAATCCACTT CTCCTACCGT GCCTGCTTCT 
AAAATTCCCG CTTTTTCCAC TAGTACGGGA AAAAGCTTGT CCCAGTCTGA TTCTACTAAT 
AATGTTAATT CTTCTTCTCC TTTATCTTCT TCCCTTCCCA CCAGCAAATC CTCTATCCCT 
CCTCCTCGAT CCAGTTCCAT CCCCTCCTCC CACATCCCCT CTATTTCTAA CGGTTCTTTA 
AAATATGCCC CTCCCACTCA CAGCACTAAA GGCCTCTCTA TTCCCACACA GACACAAAAT 
GGTCGACCTT CCACTTCTTC TTCCTCCTCC TGTTCCCACT CCCCCTTGTC CCCGACTATG 
TTGAGTCAGA GTGCGAAGAG TATCCGTACC ATTCACACAC CCAGCTTCAA CAGCTACAGT 
CGGCCGCACA ACGGAAGTGC CGGTAAATCA GCTATACCAA CGGTAACTGT CGCTAAGGAT 
GCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295579.1
CDS	615..6623
Translation	MSKPSRLARPSSAGPKLSSSRRDTPGGKPRMPVVEEKVMRIGSEGSIVGHRPPVNGTTHSQILQSAHLKEEDEASQSKSRISPPKGSTTNVSQQQVTQPKPLKGNLKVTSSEDSEHLTRKQQVVSANSSGNRCEAKSSRAGVPRRHTVGGARSSREILAMQPSDMDKKREAFLEHLKQKYPHHASAIMGHQERLREQTRGSKQSVGCPSPVPVFVDQVEHGSLVSLDALEVMSECDMPLAFTRGSRSRASLPVVRSTNQTKDRSLGVLYLQYGDDTKQIRMPNEITSVDTIRALFVSAFPHQLNMKMLESPSTAIYVKDETRNVYYELTDVRSITDHSCLKVYHKDPAHAFSHGPRPSNGDTRVHREMIFAGRDGHPLRNPPMGPPPPHMQGSMSPTTPHGMPPSPSRIPFGPRNPGGGVATMPRERAGHTLPAPARASSPCPSAILERRDVKPDEDVAAKTMSLNTRAESHYADPYMLLHHAPPPDTVDHAYHRATMRSYSNPSVPLEPPDHLFRQKSRNSQLPMLGSKTPPPSPHRMGEMRMVDIHTVQPQSSMTLERASPVRQSFRKEAPPMDAMVKARGGMGSPATPDLQVHNPLAPPTDPQTRERPVKSGSPAHSTSSTGGSPVLAPKVVAAPPERSPAPTPPLGPAPLHVNLHLFRKNVSELRLQLQHMRQLQLQNQECVRAQIKRAEQEIGVKLAEMLRRQDDPTQKQRMLVEEERHRYLSMQERVLTQLGDLEQFVETLRTDSGPAASQRAVTLKEVEDGAVSLRKVGENLAGLKGEFPALQSRMRSVLRVEVEAVKFLKEEPHKLDSMLKRVRTLTDTLSNLRRCATDNLLKPTDQVCSTPPASEKPELIESPEPAPVTPQSSIVRSEVMTSNPVVIHHAQASPVASQQSQQSTAPSPIPTQRRESTPSPTKQLRKPSLEQTQNNTSNGVSANGVHGKGPSPNLIIEEIHASHSKNKNRAVSIEAAEKEWEEKRQNLGHYDGQEFERILQEAEANMLRGIPNLEVPSQLEGSPAPPAMLPASLEEVVEINEPVAAPREHDDNQPNSEKPAKTASEKPPKPLEKPVKPALEKPLKPSLSIKSNLERQSKTQEKVKLQSTEKTNKSPPPPPPRRNYTTNTGMTTTRSGEVIYTSRKESVTEGEEESNISTMSQPEPKTPPEVKPKPITPPPISASAITEEEDEGDKIMAELQVFQKCSVKEVGLKGLVEPQIRELRPGVLLPLKDKKNTENQDDKNTDTDENGNNTIRQSPGVIYYVTAQISKGTPPPSEESTERRDASQSFPSQVSQINASDQSQSQQQVSNNKSDALKNIGLANFSGTQQQNQLSRSASLKLHPASSWEEVPGSPTQGKVHVVKAAQVQVSIEERVESPTAVCSPVSFGDSEPILSKVIPVETALTNQKLPFGSSKVKERKFEQMEENNEAFLSPDLSGEEPPPPPPDNFAFMITNTKVQALSTGEYHQLVNAKKGSVQTVTFGNKQPSASYSGMASGGDANTNGPPGADNTSKKPVIIIFDEPMDIRSAYKRLSTVFECEEELDRMLAEERIDEESEETEEEEWTRGEKVKQNGVERQSVKRTLADVTDGEVKNSSSSLLVAGDAKQDGKKKFKLKFPKKQLAALTQAIRAGSKTGKKTLQVVVYEDEEESDGTVKQHKETKRYEIASSKTESSKSNTQESNGRTDEIRKSTYKTLDSLEQTIKQLENTICEMGPTSSEDTESLTNSETQENKVVENSGHSEKGLSSKTLVPRLSAASKGPSLRKKTKPQLLPRPTNIPTTGVSVTPVATHQNANVVSPTSRMPVPVSVKARQPSSTSEKERATKPQKLQDSQRQFRQANGSAKRSGGDPKSTSPTVPASKIPAFSTSTGKSLSQSDSTNNVNSSSPLSSSLPTSKSSIPPPRSSSIPSSHIPSISNGSLKYAPPTHSTKGLSIPTQTQNGRPSTSSSSSCSHSPLSPTMLSQSAKSIRTIHTPSFNSYSRPHNGSAGKSAIPTVTVAKDAA

Target ORF information:

RefSeq Version	XM_009297304.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch211-285f17.1 (si:ch211-285f17.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297304.2

ORF Insert Sequence:

ATGTCTAAAC CGTCAAGACT TGCCCGTCCG TCCTCTGCGG GTCCCAAACT CTCGTCGTCC 
CGTCGGGATA CACCTGGTGG AAAACCCAGA ATGCCTGTAG TAGAGGAGAA GGTGATGCGG 
ATTGGAAGCG AGGGCAGTAT TGTCGGTCAT AGGCCTCCTG TCAATGGGAC GACACATTCA 
CAGATTTTGC AAAGCGCTCA TTTAAAGGAA GAGGATGAGG CGTCCCAATC AAAGAGCAGA 
ATCAGTCCAC CTAAAGGGTC CACAACGAAT GTATCCCAGC AACAGGTCAC ACAGCCCAAG 
CCTCTGAAGG GTAATCTAAA GGTGACTTCC AGTGAAGACA GCGAACACCT GACCCGTAAA 
CAGCAGGTTG TCTCTGCTAA CAGCTCTGGT AACCGCTGTG AGGCCAAATC CAGCCGCGCA 
GGGGTTCCAC GCAGACACAC TGTGGGTGGA GCGCGCAGCT CACGGGAGAT CCTGGCCATG 
CAGCCCTCGG ATATGGATAA AAAACGAGAG GCTTTTCTGG AACATCTGAA GCAGAAGTAT 
CCGCACCACG CCTCTGCCAT AATGGGCCAT CAGGAGAGAC TCAGAGAACA GACCCGGGGC 
TCTAAGCAGA GTGTGGGGTG TCCGAGTCCA GTGCCTGTTT TTGTGGATCA GGTAGAGCAC 
GGCTCACTGG TCTCTCTAGA TGCTCTGGAG GTCATGTCTG AGTGTGATAT GCCCCTCGCC 
TTCACCCGAG GCTCCCGCTC TCGAGCCAGT CTGCCAGTGG TCCGCTCTAC TAACCAGACC 
AAGGATCGCT CACTAGGAGT GCTGTATCTG CAGTATGGGG ACGACACAAA GCAGATCCGC 
ATGCCTAATG AGATAACAAG TGTTGACACA ATCAGAGCCC TGTTCGTTAG TGCCTTCCCT 
CATCAGCTCA ACATGAAGAT GCTGGAGTCG CCCAGCACTG CCATCTACGT CAAAGATGAG 
ACCAGAAATG TGTATTATGA ACTCACCGAT GTCAGGAGCA TCACAGATCA CTCCTGCCTG 
AAAGTGTATC ATAAAGACCC AGCACACGCC TTCAGTCACG GACCCCGGCC GAGCAATGGA 
GACACAAGGG TTCACAGGGA AATGATATTT GCTGGACGGG ATGGTCACCC ACTCCGGAAC 
CCCCCAATGG GTCCTCCACC CCCCCACATG CAAGGCTCCA TGTCACCCAC TACACCTCAC 
GGGATGCCTC CCTCTCCATC TCGAATTCCC TTTGGCCCGC GAAACCCAGG TGGAGGTGTT 
GCCACAATGC CACGTGAGCG AGCCGGACAC ACACTCCCAG CCCCTGCCCG AGCATCCTCC 
CCCTGCCCGA GTGCCATCTT GGAGCGGCGG GATGTCAAAC CTGACGAGGA TGTAGCTGCG 
AAGACCATGT CTCTGAACAC CAGAGCTGAA AGTCATTACG CCGACCCTTA TATGTTACTT 
CACCATGCCC CGCCCCCTGA CACAGTAGAC CACGCCTACC ACCGTGCTAC CATGCGCTCT 
TACAGCAACC CCAGCGTTCC CTTGGAGCCC CCAGACCATC TCTTCCGACA GAAAAGCAGG 
AATAGCCAAC TCCCCATGCT TGGCTCCAAA ACCCCGCCCC CTTCCCCGCA CCGAATGGGT 
GAGATGAGAA TGGTGGACAT CCATACGGTC CAACCGCAGA GCTCCATGAC TCTGGAAAGG 
GCTTCACCTG TTAGACAATC ATTTCGGAAG GAGGCTCCTC CTATGGATGC CATGGTAAAG 
GCAAGAGGGG GAATGGGGTC TCCAGCCACC CCCGATCTTC AGGTTCACAA TCCTCTGGCG 
CCCCCGACAG ACCCTCAAAC ACGCGAAAGA CCAGTGAAGT CTGGATCACC AGCTCACAGC 
ACATCTAGCA CAGGAGGCTC TCCAGTATTA GCACCAAAGG TTGTTGCTGC TCCTCCTGAG 
CGTAGCCCCG CCCCCACACC ACCTTTAGGA CCTGCCCCTT TGCATGTTAA CCTTCATCTG 
TTCAGGAAGA ATGTCTCTGA GCTCCGCCTC CAACTACAAC ATATGAGACA ACTACAGCTT 
CAGAATCAGG AGTGTGTACG TGCTCAGATA AAGCGCGCAG AACAGGAGAT CGGTGTGAAA 
CTGGCTGAAA TGCTGAGACG ACAGGATGAT CCGACACAGA AACAGAGGAT GCTTGTGGAG 
GAAGAGAGAC ACCGATATCT CAGCATGCAG GAAAGAGTTC TCACACAGCT GGGGGATCTG 
GAGCAGTTTG TGGAGACTCT GAGGACTGAT TCGGGTCCTG CTGCGTCTCA GAGAGCAGTG 
ACGCTGAAGG AGGTTGAGGA TGGGGCTGTC AGCCTGAGGA AGGTGGGAGA AAACCTGGCT 
GGACTCAAAG GTGAGTTTCC GGCTCTTCAG TCCCGCATGC GCTCAGTGCT CAGGGTTGAA 
GTAGAGGCTG TAAAGTTCCT CAAAGAAGAG CCACACAAAC TGGACAGCAT GTTAAAGAGA 
GTCCGGACCC TCACTGACAC ACTCAGCAAC CTTCGCAGGT GTGCCACCGA TAACCTGCTT 
AAACCCACTG ATCAGGTTTG CTCAACACCT CCTGCCTCTG AAAAACCAGA ACTTATTGAA 
TCCCCAGAAC CTGCACCGGT CACACCTCAG AGCTCCATCG TGAGGTCAGA GGTTATGACC 
TCAAACCCTG TGGTGATCCA CCATGCTCAG GCCTCTCCAG TAGCATCACA ACAATCCCAG 
CAATCAACAG CACCTTCACC CATCCCTACA CAAAGAAGAG AGTCTACACC GTCACCCACT 
AAACAGCTCA GAAAACCCTC ACTGGAACAA ACACAAAACA ACACCAGTAA TGGAGTTTCT 
GCTAATGGCG TACATGGCAA GGGTCCTTCT CCAAACCTGA TCATTGAGGA GATCCACGCC 
AGCCACAGCA AGAACAAGAA CAGAGCTGTG TCCATAGAGG CTGCAGAGAA GGAATGGGAG 
GAGAAAAGGC AGAACTTGGG TCATTATGAT GGACAGGAGT TTGAGCGGAT TCTCCAGGAA 
GCAGAAGCAA ACATGCTGAG GGGAATACCA AACCTCGAGG TGCCCAGTCA ACTTGAGGGT 
TCTCCTGCTC CACCTGCCAT GCTTCCTGCC TCTCTGGAGG AGGTAGTAGA AATAAACGAA 
CCAGTAGCAG CACCAAGAGA ACACGATGAC AATCAGCCAA ACTCTGAGAA ACCAGCTAAG 
ACTGCTTCAG AGAAACCTCC CAAACCTCTG GAGAAACCAG TTAAGCCAGC CTTGGAGAAA 
CCTCTTAAAC CCAGCCTATC CATCAAATCC AACCTAGAGA GACAGAGTAA GACTCAGGAA 
AAAGTTAAAC TTCAGAGCAC AGAGAAAACC AACAAGTCTC CTCCTCCACC CCCACCTCGA 
CGCAACTACA CCACCAACAC CGGCATGACC ACCACCCGCT CTGGAGAGGT CATTTACACC 
AGCAGGAAAG AGTCTGTAAC GGAAGGTGAA GAGGAGTCCA ACATTTCAAC AATGTCTCAG 
CCTGAACCAA AGACCCCCCC AGAGGTAAAG CCCAAACCCA TCACCCCTCC ACCTATCTCT 
GCCTCAGCCA TCACTGAAGA AGAGGACGAA GGAGACAAGA TTATGGCAGA GCTACAGGTT 
TTCCAGAAGT GCTCTGTTAA GGAGGTAGGG CTCAAGGGTT TGGTAGAGCC ACAGATCAGA 
GAGCTAAGAC CTGGGGTCTT ACTGCCCCTT AAAGATAAGA AGAACACAGA GAACCAAGAT 
GACAAGAATA CTGACACAGA TGAGAATGGC AACAATACTA TTCGTCAAAG TCCTGGGGTC 
ATCTACTATG TCACAGCTCA GATCTCCAAG GGAACTCCAC CACCGTCAGA GGAATCAACT 
GAGCGTAGAG ATGCATCGCA GTCTTTCCCA TCACAGGTGT CACAAATCAA TGCTTCTGAC 
CAATCCCAAA GCCAGCAGCA GGTTTCCAAT AACAAATCAG ATGCCCTCAA AAACATTGGC 
CTAGCCAACT TTTCTGGCAC CCAGCAACAA AATCAGCTGT CTCGTTCAGC CTCACTAAAG 
TTACATCCAG CCTCTTCATG GGAAGAAGTT CCTGGTAGCC CAACACAAGG GAAGGTTCAT 
GTGGTCAAGG CTGCTCAAGT GCAGGTTAGC ATAGAGGAGA GGGTAGAGTC TCCAACAGCT 
GTTTGCTCAC CAGTCTCTTT TGGAGACTCT GAACCCATCC TTTCTAAAGT GATCCCTGTC 
GAGACAGCTT TGACAAATCA AAAGCTTCCA TTTGGTTCAT CCAAGGTGAA GGAAAGGAAA 
TTTGAGCAAA TGGAGGAAAA TAATGAGGCT TTTCTCAGTC CCGATTTGTC AGGAGAAGAG 
CCACCTCCTC CACCACCTGA TAACTTTGCC TTTATGATAA CCAACACCAA GGTTCAAGCC 
CTCTCCACTG GAGAGTACCA TCAGCTTGTC AACGCCAAGA AAGGAAGTGT ACAGACAGTG 
ACTTTTGGCA ACAAACAACC AAGTGCTTCT TACAGTGGCA TGGCATCAGG AGGTGATGCT 
AATACCAATG GCCCTCCTGG AGCTGATAAC ACTAGTAAAA AGCCAGTTAT CATAATCTTT 
GATGAGCCAA TGGACATCCG TTCAGCTTAC AAGCGCCTCT CTACCGTTTT CGAATGTGAG 
GAGGAGCTAG ATCGGATGCT TGCAGAAGAA AGAATAGATG AGGAGAGTGA GGAAACTGAG 
GAAGAAGAAT GGACGAGAGG AGAGAAGGTC AAACAGAATG GAGTTGAAAG ACAGTCTGTA 
AAAAGAACTC TTGCAGATGT CACAGATGGT GAAGTCAAGA ACTCTTCATC ATCCTTACTG 
GTTGCTGGTG ATGCAAAGCA AGATGGGAAG AAAAAGTTTA AGTTGAAATT TCCCAAGAAA 
CAGCTAGCAG CATTAACACA AGCTATTCGG GCTGGAAGTA AAACAGGCAA AAAGACTTTG 
CAAGTTGTCG TCTATGAGGA TGAGGAAGAG TCCGATGGCA CTGTCAAGCA ACATAAAGAG 
ACCAAGAGGT ATGAAATTGC TAGCTCCAAA ACAGAATCCT CAAAATCAAA TACACAGGAA 
TCTAATGGAC GAACTGATGA GATTCGTAAG AGCACCTACA AAACACTTGA CAGTCTAGAG 
CAAACAATAA AGCAGCTAGA GAATACAATT TGCGAAATGG GACCAACGTC TTCGGAAGAC 
ACTGAAAGCC TAACGAATTC TGAAACTCAA GAAAACAAAG TAGTGGAGAA TTCAGGCCAC 
TCAGAGAAGG GACTTTCTTC CAAAACCTTG GTTCCTAGGT TGTCTGCTGC CAGTAAGGGT 
CCTAGTCTCC GAAAGAAGAC CAAACCACAG CTCCTTCCTC GGCCTACCAA CATACCAACT 
ACAGGGGTCT CAGTAACACC AGTCGCTACC CACCAGAACG CCAACGTGGT CTCACCTACT 
AGTCGGATGC CAGTGCCAGT TTCTGTCAAG GCGCGGCAGC CATCAAGCAC CTCAGAAAAA 
GAGAGGGCAA CAAAACCGCA AAAACTACAG GACTCTCAGA GGCAGTTCCG CCAGGCTAAT 
GGAAGTGCTA AGAGATCTGG AGGAGATCCC AAATCCACTT CTCCTACCGT GCCTGCTTCT 
AAAATTCCCG CTTTTTCCAC TAGTACGGGA AAAAGCTTGT CCCAGTCTGA TTCTACTAAT 
AATGTTAATT CTTCTTCTCC TTTATCTTCT TCCCTTCCCA CCAGCAAATC CTCTATCCCT 
CCTCCTCGAT CCAGTTCCAT CCCCTCCTCC CACATCCCCT CTATTTCTAA CGGTTCTTTA 
AAATATGCCC CTCCCACTCA CAGCACTAAA GGCCTCTCTA TTCCCACACA GACACAAAAT 
GGTCGACCTT CCACTTCTTC TTCCTCCTCC TGTTCCCACT CCCCCTTGTC CCCGACTATG 
TTGAGTCAGA GTGCGAAGAG TATCCGTACC ATTCACACAC CCAGCTTCAA CAGCTACAGT 
CGGCCGCACA ACGGAAGTGC CGGTAAATCA GCTATACCAA CGGTAACTGT CGCTAAGGAT 
GCTGCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	si:ch211-285f17.1
Entrez Gene ID	562465
Full Name	si:ch211-285f17.1
Synonyms	fj77d08,id:ibd5057,wu:fj77d08
General protein information	Preferred Names si:ch211-285f17.1 Names si:ch211-285f17.1 sickle tail protein homolog sickle tail protein sickle tail-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

	LOC705851	XP_001099259.1
Homo sapiens (human)	KIAA1217	NP_062536.2
Rattus norvegicus (Norway rat)	Etl4	XP_006254390.1
Gallus gallus (chicken)	KIAA1217	XP_004939283.1
Danio rerio (zebrafish)	si:ch211-285f17.1	XP_005162642.1
Mus musculus (house mouse)	Etl4	NP_001074475.1
Xenopus tropicalis (tropical clawed frog)	kiaa1217	XP_002933193.2
Pan troglodytes (chimpanzee)	KIAA1217	XP_003951649.1
Canis lupus familiaris (dog)	KIAA1217	XP_005617059.1
Bos taurus (cattle)	KIAA1217	XP_002692115.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

si:ch211-285f17.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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