mthfd2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mthfd2
Entrez Gene ID	100004977
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Synonyms	wu:fb38b11,wu:fe12e11,zgc:91891
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase Names bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001002181.1	NP_001002181.1	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MTHFD2	XP_005630608.1
Gallus gallus (chicken)	MTHFD2	NP_001026531.1
Danio rerio (zebrafish)	LOC101886265	XP_005168726.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004677	XP_001687783.1
Pan troglodytes (chimpanzee)	MTHFD2	XP_001154612.1
Bos taurus (cattle)	MTHFD2	NP_001069223.1
Mus musculus (house mouse)	Mthfd2	NP_032664.1
Xenopus tropicalis (tropical clawed frog)	mthfd2	NP_001123716.1
Rattus norvegicus (Norway rat)	Mthfd2	NP_001102868.1
Homo sapiens (human)	MTHFD2	NP_006627.2
Danio rerio (zebrafish)	mthfd2	NP_001002181.1
Drosophila melanogaster (fruit fly)	Nmdmc	NP_476929.1

Gene Ontology
Bibliography

Related articles in PubMed

TET2 plays an essential role in erythropoiesis by regulating lineage-specific genes via DNA oxidative demethylation in a zebrafish model.
Ge L, Zhang RP, Wan F, Guo DY, Wang P, Xiang LX, Shao JZ
Molecular and cellular biology34(6)989-1002(2014 Mar)

Regulation of E2F1 by the von Hippel-Lindau tumour suppressor protein predicts survival in renal cell cancer patients.
Mans DA, Vermaat JS, Weijts BG, van Rooijen E, van Reeuwijk J, Boldt K, Daenen LG, van der Groep P, Rowland BD, Jans JJ, Roepman R, Voest EE, van Diest PJ, Verhaar MC, de Bruin A, Giles RH
The Journal of pathology231(1)117-29(2013 Sep)

Disruption of the folate pathway in zebrafish causes developmental defects.
Lee MS, Bonner JR, Bernard DJ, Sanchez EL, Sause ET, Prentice RR, Burgess SM, Brody LC
BMC developmental biology1212(2012 Apr)

ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis.
Krens SF, Corredor-Ad?mez M, He S, Snaar-Jagalska BE, Spaink HP
BMC genomics9196(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mthfd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mthfd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02039	NM_001002181.1 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (mthfd2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mthfd2 ( NM_001002181.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002181.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (mthfd2), mRNA.

Price & Availability↑

CloneID	ODa02039
Clone ID Related Accession (Same CDS sequence)	NM_001002181.1
Accession Version	NM_001002181.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-18
Organism	Danio rerio(zebrafish)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC074043.1. On Dec 14, 2007 this sequence version replaced XM_001340523.1. ##Evidence-Data-START## Transcript exon combination :: BC074043.1, BC155262.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCTACAC TCCGGGCATT GCGGAAACTC TGCCGGCATT TCCACCATCA AACATGTAGT 
TTTCATTTGT CATCTTCAAG ACAAGATGCT GTGGTGATCT CCGGTCGGAA ACTGGCCCGT 
CAGATCCGCA ATGAAGCACG TGATGATGTG GAGGAGTGGG TTTCTGCTGG AAATCGACGG 
CCTCACCTGA GTGTGGTTTT AGTAGGTGAC AATCCAGCCA GTCACTCTTA CGTCCTCAAT 
AAGACCCGCG CGGCAGCTGA AGTTGGTATC AGCAGCGAGA CCATCTTGAA ACCTTCTAAT 
ATCAGCGAAG AGGAGCTGCT TGATCTCATT GTGAAACTAA ACTCAGATCA CAGAGTAGAC 
GGGCTGTTGG TTCAGCTGCC ACTTCCTGAT CACATCGATG AGCGGCGTGT ATGTAATGCA 
GTGTGCCCGG GGAAAGATGT TGATGGTTTC CATGTGGTCA ATGTGGGTCG CATGTGCCTG 
GATCAGTCAA CTATGTTACC CGCCACTCCA TGGGGAGTTT GGGAGATTAT CAAACGCACA 
GGCATAGAGA CGCTGGGAAA GAATGTGTTG GTGGCTGGCC GCTCCAAGAA CGTGGGGATG 
CCCATCGCAA TGCTGCTACA CACCGACGGA CGACACGAAA GGCCCGGAGG TGATGCCACA 
GTGACAATCT CCCACAGATA CACTCCCAAA GAACAGCTCC GACAGCACAC TAAAATCGCA 
GATATTATCG TCGCCGCTGC AGGTATTCCT AACCTCATCA CGGCTGATAT GATAAAGGAG 
GGTGCAGCAG TTATAGATGT CGGGATCAAC AGAATATTGG ACCCCATGAC TGGGAAAAAC 
AGACTTGTTG GTGACGTGGA CTTTGAAGGT GTCAGAAAGA AGGCCAGCTT CATTACTCCA 
GTTCCCGGCG GCGTTGGCCC CATGACTGTA GCCATGCTGA TGAAAAACAC AATCATAGCT 
GCAAAAAATA TGATTTTAAC TCCACCTCAG AGAGTCCGAA TGGCAGCGTC TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002181.1
CDS	162..1178
Translation	MATLRALRKLCRHFHHQTCSFHLSSSRQDAVVISGRKLARQIRNEARDDVEEWVSAGNRRPHLSVVLVGDNPASHSYVLNKTRAAAEVGISSETILKPSNISEEELLDLIVKLNSDHRVDGLLVQLPLPDHIDERRVCNAVCPGKDVDGFHVVNVGRMCLDQSTMLPATPWGVWEIIKRTGIETLGKNVLVAGRSKNVGMPIAMLLHTDGRHERPGGDATVTISHRYTPKEQLRQHTKIADIIVAAAGIPNLITADMIKEGAAVIDVGINRILDPMTGKNRLVGDVDFEGVRKKASFITPVPGGVGPMTVAMLMKNTIIAAKNMILTPPQRVRMAASS

Target ORF information:

RefSeq Version	NM_001002181.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (mthfd2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002181.1

ORF Insert Sequence:

ATGGCTACAC TCCGGGCATT GCGGAAACTC TGCCGGCATT TCCACCATCA AACATGTAGT 
TTTCATTTGT CATCTTCAAG ACAAGATGCT GTGGTGATCT CCGGTCGGAA ACTGGCCCGT 
CAGATCCGCA ATGAAGCACG TGATGATGTG GAGGAGTGGG TTTCTGCTGG AAATCGACGG 
CCTCACCTGA GTGTGGTTTT AGTAGGTGAC AATCCAGCCA GTCACTCTTA CGTCCTCAAT 
AAGACCCGCG CGGCAGCTGA AGTTGGTATC AGCAGCGAGA CCATCTTGAA ACCTTCTAAT 
ATCAGCGAAG AGGAGCTGCT TGATCTCATT GTGAAACTAA ACTCAGATCA CAGAGTAGAC 
GGGCTGTTGG TTCAGCTGCC ACTTCCTGAT CACATCGATG AGCGGCGTGT ATGTAATGCA 
GTGTGCCCGG GGAAAGATGT TGATGGTTTC CATGTGGTCA ATGTGGGTCG CATGTGCCTG 
GATCAGTCAA CTATGTTACC CGCCACTCCA TGGGGAGTTT GGGAGATTAT CAAACGCACA 
GGCATAGAGA CGCTGGGAAA GAATGTGTTG GTGGCTGGCC GCTCCAAGAA CGTGGGGATG 
CCCATCGCAA TGCTGCTACA CACCGACGGA CGACACGAAA GGCCCGGAGG TGATGCCACA 
GTGACAATCT CCCACAGATA CACTCCCAAA GAACAGCTCC GACAGCACAC TAAAATCGCA 
GATATTATCG TCGCCGCTGC AGGTATTCCT AACCTCATCA CGGCTGATAT GATAAAGGAG 
GGTGCAGCAG TTATAGATGT CGGGATCAAC AGAATATTGG ACCCCATGAC TGGGAAAAAC 
AGACTTGTTG GTGACGTGGA CTTTGAAGGT GTCAGAAAGA AGGCCAGCTT CATTACTCCA 
GTTCCCGGCG GCGTTGGCCC CATGACTGTA GCCATGCTGA TGAAAAACAC AATCATAGCT 
GCAAAAAATA TGATTTTAAC TCCACCTCAG AGAGTCCGAA TGGCAGCGTC TTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TET2 plays an essential role in erythropoiesis by regulating lineage-specific genes via DNA oxidative demethylation in a zebrafish model.
Molecular and cellular biology34(6)989-1002(2014 Mar)
Ge L,Zhang RP,Wan F,Guo DY,Wang P,Xiang LX,Shao JZ

Regulation of E2F1 by the von Hippel-Lindau tumour suppressor protein predicts survival in renal cell cancer patients.
The Journal of pathology231(1)117-29(2013 Sep)
Mans DA,Vermaat JS,Weijts BG,van Rooijen E,van Reeuwijk J,Boldt K,Daenen LG,van der Groep P,Rowland BD,Jans JJ,Roepman R,Voest EE,van Diest PJ,Verhaar MC,de Bruin A,Giles RH

Disruption of the folate pathway in zebrafish causes developmental defects.
BMC developmental biology1212(2012 Apr)
Lee MS,Bonner JR,Bernard DJ,Sanchez EL,Sause ET,Prentice RR,Burgess SM,Brody LC

ERK1 and ERK2 MAPK are key regulators of distinct gene sets in zebrafish embryogenesis.
BMC genomics9196(2008 Apr)
Krens SF,Corredor-Ad?mez M,He S,Snaar-Jagalska BE,Spaink HP