CLCA cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLCA
Entrez Gene ID	101210008
Full Name	chloride channel protein CLC-b-like
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001280747.1	NP_001267676.1	chloride channel protein CLC-b-like

Pathways from BioSystems
Gene Ontology

The following CLCA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLCA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu36505	NM_001280747.1 Latest version!	Cucumis sativus chloride channel protein CLC-b-like (CLCA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLCA ( NM_001280747.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001267676.1 Cucumis sativus chloride channel protein CLC-b-like (CLCA), mRNA.

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CloneID	OCu36505
Clone ID Related Accession (Same CDS sequence)	NM_001280747.1
Accession Version	NM_001280747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cucumis sativus(cucumber)
Product	chloride channel protein CLC-b-like
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JQ513914.1. On or before Jul 23, 2013 this sequence version replaced XM_004168613.1, XM_004138565.1. ##Evidence-Data-START## Transcript exon combination :: JQ513914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02570546, SAMN02570552 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAGA ATCCAAGCTT TGTTACAGAA TCAACTCTCA CAAACTCCAT GGAAGCAGAA 
CCTCAAGATG AAGAAAGAGA CCCAGAAAGC AATCCTTTAA ACCAACCCCT TCTCAAGAGA 
AACAGAACTC TCTCTTCTAG TCCTCTTGCC ATTGTTGGGG CAAAAGTTTC TCTCATTGAA 
AGCTTGGATT ACGAGATCAA TGAGAACGAT CTGTTTAAGC ATGATTGGAG AAGCAGATCC 
AAAGTTCAAG TGCTGCAGTA CATATTCTCT AAATGGACTT TGGCTTGTCT TGTTGGACTT 
CTTACAGGGA TCATTGCCAC TCTCATCAAT CTTGCCATTG AAAATATTGC TGGCTACAAG 
CTTTTGAAAG TGGTTGATTA CATAAAGGAA GAAAGGTACT TAATGGGGTT CGCATACTTT 
ACCACTGCCA ACTTCCTTCT GACTTTTGTT GCTGCTGCTC TCTGTGTATG CTTTGCTCCC 
ACCGCAGCTG GGCCTGGCAT ACCAGAAATT AAAGCCTATC TCAATGGCAT AGACACTCCT 
AATATGTTCG GCGCCACGAC GTTGATCGTT AAGATTGTTG GAAGCATTGG AGCAGTGGCA 
GCTGGGCTAG ATCTTGGGAA AGAAGGGCCT CTAGTACACA TTGGCAGCTG CATTGCTTCT 
TTACTTGGAC AGGGTGGACC AGATAACTAT CGAGTCAAAT GGACATGGCT ACGCTATTTC 
AACAATGATC GTGATCGTCG GGATCTTATC ACATGCGGGG CATCATCAGG GGTTTGTGCA 
GCTTTTCGGG CTCCAGTTGG GGGTGTCCTA TTTGCTCTAG AAGAGGTGGC TACTTGGTGG 
AGGAGTGCCC TGCTATGGAG GACCTTCTTC AGCACAGCCA TTGTTGTGGT AGTGCTAAGA 
ACTTTCATTG AAATATGCAA AGCAGGTGAT TGTGGGCTGT TTGGAGAAGG AGGGCTCATC 
ATGTTTGATG TCAGTGGTGT TTCTGTAAGT TACCATATCA TGGACATTAT TCCAGTTGCT 
ATCATTGGCC TTCTTGGTGG ATTCCTTGGA AGCCTTTACA ATCATCTTCT CCACAAAATC 
CTCAGAGTTT ACAACCTCAT CAATCAGAAA GGGAGAATGC ACAAACTATT ACTAGCCTTA 
GCAGTATCAC TTTTCACTTC AATTTGCCAA TACAGTTTGC CATACCTTGT TCAATGCACT 
CCCTGCAACT CTTCTCTTTC AGATTCAGCT TGCCCAACCA ATGGCCGGTC CGGCAACTTC 
AAGCAATTCA ACTGTCCTAA AGGCTATTAC AATGATCTCG CTACCCTTCT TCTCACCACC 
AACGACGACG CTGTTCGAAA CATCTTCTCC ATCAACACTC CAGCCGAGTA TCAGCCTCTT 
TCCCTTGTCA TCTTCTTCCT TCTCTACTGC ATATTAGGGT TATTCACCTT CGGTATCGCC 
GTCCCTTCCG GTCTCTTCCT TCCGATCATC TTGATGGGCT CTGGCTATGG CCGCCTCATC 
GGCCTCCTCA TGCGACCCTA CACCAATCTC GATCAAGGAC TCTTAGCTGT TCTCGGTGCT 
GCTTCCTTAA TGGCCGGCTC CATGCGAATG ACCGTTTCTC TTTGTGTCAT ATTCCTCGAA 
CTCACCAACA ATCTCCTTCT CCTTCCGATC ACCATGATTG TTCTTCTAAT TGCAAAAACA 
GTCGGAGACA GTTTTAATCC TAGCATTTAC GACATCATTC TCCACCTCAA AGGACTTCCA 
TTCCTCGACG CGAATCCGGA GCCATGGATG AGAAACATAA CCGTCGGTGA GCTCGCCGAC 
GCGAAACCAG CGGTGGTGAC ACTTCGGGGA TTAGAGAAAG TATCACGCAT TGTGGAGGTT 
CTTAGGAACA CAACACACAA TGGATTCCCT GTTGTGGATG CCGATGCGGT TGTGCCGCCG 
GTGGGCATGG CAGTGGGAGC GACGGAGCTG CACGGACTAG TACTGAGAGC GCATCTACTA 
CAAGTGCTGA AGAAGAAATG GTTCTTGAGA GAGAGGAGGA GGACGGAGGA CTGGGAAGTG 
AGGGAGAAAT TTACATGGGT GGAATTGGCG GAGAGGGAAG GAAAGATCGA AGAGCTTGTA 
GTGACGAAAG AGGAAATGGA AATGTACGTT GATCTTCATC CATTGACGAA CACTACACCG 
TATACAGTAC TGGAAAGCAT GTCGGTGGCA AAAGCTTTGG TTCTTTTCCG GCAAGTAGGA 
CTCCGCCATT TGTTGATCGT CCCCAAATAT GAAGCAGCCG GAGTTCCGCC GGTGATTGGG 
ATCTTGACGA GGCAAGATTT AAGGCCATAC AATATATTGA GTGCATTTCC TGATTTGGCA 
AGGATCAAAG GCAATGAAAA ACGGAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001267676.1
CDS	1..2370
Translation	MDENPSFVTESTLTNSMEAEPQDEERDPESNPLNQPLLKRNRTLSSSPLAIVGAKVSLIESLDYEINENDLFKHDWRSRSKVQVLQYIFSKWTLACLVGLLTGIIATLINLAIENIAGYKLLKVVDYIKEERYLMGFAYFTTANFLLTFVAAALCVCFAPTAAGPGIPEIKAYLNGIDTPNMFGATTLIVKIVGSIGAVAAGLDLGKEGPLVHIGSCIASLLGQGGPDNYRVKWTWLRYFNNDRDRRDLITCGASSGVCAAFRAPVGGVLFALEEVATWWRSALLWRTFFSTAIVVVVLRTFIEICKAGDCGLFGEGGLIMFDVSGVSVSYHIMDIIPVAIIGLLGGFLGSLYNHLLHKILRVYNLINQKGRMHKLLLALAVSLFTSICQYSLPYLVQCTPCNSSLSDSACPTNGRSGNFKQFNCPKGYYNDLATLLLTTNDDAVRNIFSINTPAEYQPLSLVIFFLLYCILGLFTFGIAVPSGLFLPIILMGSGYGRLIGLLMRPYTNLDQGLLAVLGAASLMAGSMRMTVSLCVIFLELTNNLLLLPITMIVLLIAKTVGDSFNPSIYDIILHLKGLPFLDANPEPWMRNITVGELADAKPAVVTLRGLEKVSRIVEVLRNTTHNGFPVVDADAVVPPVGMAVGATELHGLVLRAHLLQVLKKKWFLRERRRTEDWEVREKFTWVELAEREGKIEELVVTKEEMEMYVDLHPLTNTTPYTVLESMSVAKALVLFRQVGLRHLLIVPKYEAAGVPPVIGILTRQDLRPYNILSAFPDLARIKGNEKRN

Target ORF information:

RefSeq Version	NM_001280747.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus chloride channel protein CLC-b-like (CLCA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001280747.1

ORF Insert Sequence:

ATGGATGAGA ATCCAAGCTT TGTTACAGAA TCAACTCTCA CAAACTCCAT GGAAGCAGAA 
CCTCAAGATG AAGAAAGAGA CCCAGAAAGC AATCCTTTAA ACCAACCCCT TCTCAAGAGA 
AACAGAACTC TCTCTTCTAG TCCTCTTGCC ATTGTTGGGG CAAAAGTTTC TCTCATTGAA 
AGCTTGGATT ACGAGATCAA TGAGAACGAT CTGTTTAAGC ATGATTGGAG AAGCAGATCC 
AAAGTTCAAG TGCTGCAGTA CATATTCTCT AAATGGACTT TGGCTTGTCT TGTTGGACTT 
CTTACAGGGA TCATTGCCAC TCTCATCAAT CTTGCCATTG AAAATATTGC TGGCTACAAG 
CTTTTGAAAG TGGTTGATTA CATAAAGGAA GAAAGGTACT TAATGGGGTT CGCATACTTT 
ACCACTGCCA ACTTCCTTCT GACTTTTGTT GCTGCTGCTC TCTGTGTATG CTTTGCTCCC 
ACCGCAGCTG GGCCTGGCAT ACCAGAAATT AAAGCCTATC TCAATGGCAT AGACACTCCT 
AATATGTTCG GCGCCACGAC GTTGATCGTT AAGATTGTTG GAAGCATTGG AGCAGTGGCA 
GCTGGGCTAG ATCTTGGGAA AGAAGGGCCT CTAGTACACA TTGGCAGCTG CATTGCTTCT 
TTACTTGGAC AGGGTGGACC AGATAACTAT CGAGTCAAAT GGACATGGCT ACGCTATTTC 
AACAATGATC GTGATCGTCG GGATCTTATC ACATGCGGGG CATCATCAGG GGTTTGTGCA 
GCTTTTCGGG CTCCAGTTGG GGGTGTCCTA TTTGCTCTAG AAGAGGTGGC TACTTGGTGG 
AGGAGTGCCC TGCTATGGAG GACCTTCTTC AGCACAGCCA TTGTTGTGGT AGTGCTAAGA 
ACTTTCATTG AAATATGCAA AGCAGGTGAT TGTGGGCTGT TTGGAGAAGG AGGGCTCATC 
ATGTTTGATG TCAGTGGTGT TTCTGTAAGT TACCATATCA TGGACATTAT TCCAGTTGCT 
ATCATTGGCC TTCTTGGTGG ATTCCTTGGA AGCCTTTACA ATCATCTTCT CCACAAAATC 
CTCAGAGTTT ACAACCTCAT CAATCAGAAA GGGAGAATGC ACAAACTATT ACTAGCCTTA 
GCAGTATCAC TTTTCACTTC AATTTGCCAA TACAGTTTGC CATACCTTGT TCAATGCACT 
CCCTGCAACT CTTCTCTTTC AGATTCAGCT TGCCCAACCA ATGGCCGGTC CGGCAACTTC 
AAGCAATTCA ACTGTCCTAA AGGCTATTAC AATGATCTCG CTACCCTTCT TCTCACCACC 
AACGACGACG CTGTTCGAAA CATCTTCTCC ATCAACACTC CAGCCGAGTA TCAGCCTCTT 
TCCCTTGTCA TCTTCTTCCT TCTCTACTGC ATATTAGGGT TATTCACCTT CGGTATCGCC 
GTCCCTTCCG GTCTCTTCCT TCCGATCATC TTGATGGGCT CTGGCTATGG CCGCCTCATC 
GGCCTCCTCA TGCGACCCTA CACCAATCTC GATCAAGGAC TCTTAGCTGT TCTCGGTGCT 
GCTTCCTTAA TGGCCGGCTC CATGCGAATG ACCGTTTCTC TTTGTGTCAT ATTCCTCGAA 
CTCACCAACA ATCTCCTTCT CCTTCCGATC ACCATGATTG TTCTTCTAAT TGCAAAAACA 
GTCGGAGACA GTTTTAATCC TAGCATTTAC GACATCATTC TCCACCTCAA AGGACTTCCA 
TTCCTCGACG CGAATCCGGA GCCATGGATG AGAAACATAA CCGTCGGTGA GCTCGCCGAC 
GCGAAACCAG CGGTGGTGAC ACTTCGGGGA TTAGAGAAAG TATCACGCAT TGTGGAGGTT 
CTTAGGAACA CAACACACAA TGGATTCCCT GTTGTGGATG CCGATGCGGT TGTGCCGCCG 
GTGGGCATGG CAGTGGGAGC GACGGAGCTG CACGGACTAG TACTGAGAGC GCATCTACTA 
CAAGTGCTGA AGAAGAAATG GTTCTTGAGA GAGAGGAGGA GGACGGAGGA CTGGGAAGTG 
AGGGAGAAAT TTACATGGGT GGAATTGGCG GAGAGGGAAG GAAAGATCGA AGAGCTTGTA 
GTGACGAAAG AGGAAATGGA AATGTACGTT GATCTTCATC CATTGACGAA CACTACACCG 
TATACAGTAC TGGAAAGCAT GTCGGTGGCA AAAGCTTTGG TTCTTTTCCG GCAAGTAGGA 
CTCCGCCATT TGTTGATCGT CCCCAAATAT GAAGCAGCCG GAGTTCCGCC GGTGATTGGG 
ATCTTGACGA GGCAAGATTT AAGGCCATAC AATATATTGA GTGCATTTCC TGATTTGGCA 
AGGATCAAAG GCAATGAAAA ACGGAATTGA