LOC101209860 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101209860
Entrez Gene ID	101209860
Full Name	bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004141926.3	XP_004141974.1	bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic
XM_004141926.2	XP_004141974.1	bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic

Pathways from BioSystems
Gene Ontology

The following LOC101209860 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101209860 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCu70887	XM_004141926.3 Latest version!	Cucumis sativus bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (CPC-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
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OCu70887	XM_004141926.2	Cucumis sativus bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (LOC101209860), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	Quote Price
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101209860 ( XM_004141926.3 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004141974.1 Cucumis sativus bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (CPC-1), mRNA.

Price & Availability↑

CloneID	OCu70887
Clone ID Related Accession (Same CDS sequence)	XM_004141926.3 , XM_004141926.2
Accession Version	XM_004141926.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026660.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 17, 2019 this sequence version replaced XM_004141926.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GGCCGGCATT TTCTGGCTTA GGCATCAGAA CTGAAGGGAT TAGCAGGAAT 
TCAACACTTA TTTGTGTGCC AATAATGTCA GAATCAATTG ACAAGATGAT TGTTGACGTT 
AAGAAAGCTG GAGAGAATGG TGCTGACATT GTGGAGATAA GATTGGACGC ATTGAAGATT 
TTCAATCCCC ATGAAGATCT TAAACTTCTC ATTAAGGAGT CCCCTCTACC CACCTTATTC 
ACTTACAGAC CAAATTGGGA GGGTGGTCAG TATGATGGTG ATGAAAACCA ACGACTGGAT 
GTTCTTCGAT TAGCCATGAA ATTGGGAGCA GACTATATTG ATGTTGAGCT TCAGGTGGCT 
CGGGAGTTCA TTGACTCTAT ACAGCAAGAG AAGCCAAACA ATTTTAAAGT CATTGTTTCC 
AATCACAATT ATAAGAAGAC CCCATCTTTA GAGGATTTAG GGAGTCTTGT TGCAGAAATC 
CAAGCAACTG GAGCTGACAT TGTGAAGATT GCAACGACTG CATTGGATAT CACTGATGTT 
GCTCGCATGT TTCAAGTACT TGTCTATTCA CAAGTTCCAA TAATTGGACT GGTTATGAAG 
GATAGAGGAT TCATTTGTCG ACTACTTTGC CCCAAATATG GTGGTTATCT TACTTTTGGT 
ACCCTTGAGG AGGGAATAGT TTCAGCTCCT GGTCAACCGA CCATCAAAGA TCTATTAAAT 
CTATACAACT TCAGACACAT AGGGCCTGAC ACTAAGGTCT TTGGCATTAT TGGAAACCCT 
GTAGGTGACA GCAAATTACC TGCATTGTAC AATGAGCTTT TCAAGTTAGT TGGTCTCGAC 
GCCATTTATG TTCCCTTATT GGTGGACGAT CCTACCAAGT TCTTAGATAC TTATTCATCA 
AGTGATATTG GCGGATTCAG TGTTACAATC CCACACAAGG AAGCAGTATT CAAATATTGC 
GATGAGGTTG ATTCAGTTGC AAAGGCAACT GGAGCTGTTA ACTTTGTTCT TAGAAGACAA 
TCTGATGGAA AATTATGTGG CTATAACACA GATTGTTATG GTGCTATTTC AGCCATTGAA 
GACGGATTAC TCGGCTTGCA TAACGACAGC TTCGCAGTCA GTTCACGCTT AGCTGGAAAG 
CTCGTTGTTG TCATTGGTGC TGGTGGAGTT GGCAAAGCTG TTGCTTATGG TGCAAAACAA 
AAGGGAGCCA GGATTGTAAT TGCTAACCGC TCGTTTGATC GGGCTAGAGA ACTTGCAGAC 
CGTGTTGGGG GACAGTCTGT GTCACTTGCT GACTTAGAAA ATTTTCACCC AGAGGATGGC 
ATGATTCTTG TCAACGCTAC GCCAATTGGA ATGCAGCCCA AAGTTGATGA AACTCCCATT 
CCAAAGCATG CTCTAAAACG CTACTCCCTG GTTTTCGATG CTGTTTACAC ACCAAGAATG 
ACTAGACTCT TGCGGGAAGC TGAGGAGTCT GGAGCAGCTA TTGTTCCTGG GTTGGAGATG 
TTCACCAGGC AAGCATATGA ACAATATGAA GGGTTCACCG GCCTACCTGC ACCCAAGGAA 
CGTATTAGGA AATTTTTGGC AGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004141974.1
CDS	574..2163
Translation	MEFGPAFSGLGIRTEGISRNSTLICVPIMSESIDKMIVDVKKAGENGADIVEIRLDALKIFNPHEDLKLLIKESPLPTLFTYRPNWEGGQYDGDENQRLDVLRLAMKLGADYIDVELQVAREFIDSIQQEKPNNFKVIVSNHNYKKTPSLEDLGSLVAEIQATGADIVKIATTALDITDVARMFQVLVYSQVPIIGLVMKDRGFICRLLCPKYGGYLTFGTLEEGIVSAPGQPTIKDLLNLYNFRHIGPDTKVFGIIGNPVGDSKLPALYNELFKLVGLDAIYVPLLVDDPTKFLDTYSSSDIGGFSVTIPHKEAVFKYCDEVDSVAKATGAVNFVLRRQSDGKLCGYNTDCYGAISAIEDGLLGLHNDSFAVSSRLAGKLVVVIGAGGVGKAVAYGAKQKGARIVIANRSFDRARELADRVGGQSVSLADLENFHPEDGMILVNATPIGMQPKVDETPIPKHALKRYSLVFDAVYTPRMTRLLREAEESGAAIVPGLEMFTRQAYEQYEGFTGLPAPKERIRKFLADN

Target ORF information:

RefSeq Version	XM_004141926.3
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (CPC-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004141926.3

ORF Insert Sequence:

ATGGAGTTTG GGCCGGCATT TTCTGGCTTA GGCATCAGAA CTGAAGGGAT TAGCAGGAAT 
TCAACACTTA TTTGTGTGCC AATAATGTCA GAATCAATTG ACAAGATGAT TGTTGACGTT 
AAGAAAGCTG GAGAGAATGG TGCTGACATT GTGGAGATAA GATTGGACGC ATTGAAGATT 
TTCAATCCCC ATGAAGATCT TAAACTTCTC ATTAAGGAGT CCCCTCTACC CACCTTATTC 
ACTTACAGAC CAAATTGGGA GGGTGGTCAG TATGATGGTG ATGAAAACCA ACGACTGGAT 
GTTCTTCGAT TAGCCATGAA ATTGGGAGCA GACTATATTG ATGTTGAGCT TCAGGTGGCT 
CGGGAGTTCA TTGACTCTAT ACAGCAAGAG AAGCCAAACA ATTTTAAAGT CATTGTTTCC 
AATCACAATT ATAAGAAGAC CCCATCTTTA GAGGATTTAG GGAGTCTTGT TGCAGAAATC 
CAAGCAACTG GAGCTGACAT TGTGAAGATT GCAACGACTG CATTGGATAT CACTGATGTT 
GCTCGCATGT TTCAAGTACT TGTCTATTCA CAAGTTCCAA TAATTGGACT GGTTATGAAG 
GATAGAGGAT TCATTTGTCG ACTACTTTGC CCCAAATATG GTGGTTATCT TACTTTTGGT 
ACCCTTGAGG AGGGAATAGT TTCAGCTCCT GGTCAACCGA CCATCAAAGA TCTATTAAAT 
CTATACAACT TCAGACACAT AGGGCCTGAC ACTAAGGTCT TTGGCATTAT TGGAAACCCT 
GTAGGTGACA GCAAATTACC TGCATTGTAC AATGAGCTTT TCAAGTTAGT TGGTCTCGAC 
GCCATTTATG TTCCCTTATT GGTGGACGAT CCTACCAAGT TCTTAGATAC TTATTCATCA 
AGTGATATTG GCGGATTCAG TGTTACAATC CCACACAAGG AAGCAGTATT CAAATATTGC 
GATGAGGTTG ATTCAGTTGC AAAGGCAACT GGAGCTGTTA ACTTTGTTCT TAGAAGACAA 
TCTGATGGAA AATTATGTGG CTATAACACA GATTGTTATG GTGCTATTTC AGCCATTGAA 
GACGGATTAC TCGGCTTGCA TAACGACAGC TTCGCAGTCA GTTCACGCTT AGCTGGAAAG 
CTCGTTGTTG TCATTGGTGC TGGTGGAGTT GGCAAAGCTG TTGCTTATGG TGCAAAACAA 
AAGGGAGCCA GGATTGTAAT TGCTAACCGC TCGTTTGATC GGGCTAGAGA ACTTGCAGAC 
CGTGTTGGGG GACAGTCTGT GTCACTTGCT GACTTAGAAA ATTTTCACCC AGAGGATGGC 
ATGATTCTTG TCAACGCTAC GCCAATTGGA ATGCAGCCCA AAGTTGATGA AACTCCCATT 
CCAAAGCATG CTCTAAAACG CTACTCCCTG GTTTTCGATG CTGTTTACAC ACCAAGAATG 
ACTAGACTCT TGCGGGAAGC TGAGGAGTCT GGAGCAGCTA TTGTTCCTGG GTTGGAGATG 
TTCACCAGGC AAGCATATGA ACAATATGAA GGGTTCACCG GCCTACCTGC ACCCAAGGAA 
CGTATTAGGA AATTTTTGGC AGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101209860 ( XM_004141926.2 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_004141974.1 Cucumis sativus bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (LOC101209860), mRNA.

Price & Availability↑

CloneID	OCu70887
Clone ID Related Accession (Same CDS sequence)	XM_004141926.3 , XM_004141926.2
Accession Version	XM_004141926.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1590bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-22
Organism	Cucumis sativus(cucumber)
Product	bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011953808.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 23, 2015 this sequence version replaced XM_004141926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cucumis sativus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GGCCGGCATT TTCTGGCTTA GGCATCAGAA CTGAAGGGAT TAGCAGGAAT 
TCAACACTTA TTTGTGTGCC AATAATGTCA GAATCAATTG ACAAGATGAT TGTTGACGTT 
AAGAAAGCTG GAGAGAATGG TGCTGACATT GTGGAGATAA GATTGGACGC ATTGAAGATT 
TTCAATCCCC ATGAAGATCT TAAACTTCTC ATTAAGGAGT CCCCTCTACC CACCTTATTC 
ACTTACAGAC CAAATTGGGA GGGTGGTCAG TATGATGGTG ATGAAAACCA ACGACTGGAT 
GTTCTTCGAT TAGCCATGAA ATTGGGAGCA GACTATATTG ATGTTGAGCT TCAGGTGGCT 
CGGGAGTTCA TTGACTCTAT ACAGCAAGAG AAGCCAAACA ATTTTAAAGT CATTGTTTCC 
AATCACAATT ATAAGAAGAC CCCATCTTTA GAGGATTTAG GGAGTCTTGT TGCAGAAATC 
CAAGCAACTG GAGCTGACAT TGTGAAGATT GCAACGACTG CATTGGATAT CACTGATGTT 
GCTCGCATGT TTCAAGTACT TGTCTATTCA CAAGTTCCAA TAATTGGACT GGTTATGAAG 
GATAGAGGAT TCATTTGTCG ACTACTTTGC CCCAAATATG GTGGTTATCT TACTTTTGGT 
ACCCTTGAGG AGGGAATAGT TTCAGCTCCT GGTCAACCGA CCATCAAAGA TCTATTAAAT 
CTATACAACT TCAGACACAT AGGGCCTGAC ACTAAGGTCT TTGGCATTAT TGGAAACCCT 
GTAGGTGACA GCAAATTACC TGCATTGTAC AATGAGCTTT TCAAGTTAGT TGGTCTCGAC 
GCCATTTATG TTCCCTTATT GGTGGACGAT CCTACCAAGT TCTTAGATAC TTATTCATCA 
AGTGATATTG GCGGATTCAG TGTTACAATC CCACACAAGG AAGCAGTATT CAAATATTGC 
GATGAGGTTG ATTCAGTTGC AAAGGCAACT GGAGCTGTTA ACTTTGTTCT TAGAAGACAA 
TCTGATGGAA AATTATGTGG CTATAACACA GATTGTTATG GTGCTATTTC AGCCATTGAA 
GACGGATTAC TCGGCTTGCA TAACGACAGC TTCGCAGTCA GTTCACGCTT AGCTGGAAAG 
CTCGTTGTTG TCATTGGTGC TGGTGGAGTT GGCAAAGCTG TTGCTTATGG TGCAAAACAA 
AAGGGAGCCA GGATTGTAAT TGCTAACCGC TCGTTTGATC GGGCTAGAGA ACTTGCAGAC 
CGTGTTGGGG GACAGTCTGT GTCACTTGCT GACTTAGAAA ATTTTCACCC AGAGGATGGC 
ATGATTCTTG TCAACGCTAC GCCAATTGGA ATGCAGCCCA AAGTTGATGA AACTCCCATT 
CCAAAGCATG CTCTAAAACG CTACTCCCTG GTTTTCGATG CTGTTTACAC ACCAAGAATG 
ACTAGACTCT TGCGGGAAGC TGAGGAGTCT GGAGCAGCTA TTGTTCCTGG GTTGGAGATG 
TTCACCAGGC AAGCATATGA ACAATATGAA GGGTTCACCG GCCTACCTGC ACCCAAGGAA 
CGTATTAGGA AATTTTTGGC AGATAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004141974.1
CDS	414..2003
Translation	MEFGPAFSGLGIRTEGISRNSTLICVPIMSESIDKMIVDVKKAGENGADIVEIRLDALKIFNPHEDLKLLIKESPLPTLFTYRPNWEGGQYDGDENQRLDVLRLAMKLGADYIDVELQVAREFIDSIQQEKPNNFKVIVSNHNYKKTPSLEDLGSLVAEIQATGADIVKIATTALDITDVARMFQVLVYSQVPIIGLVMKDRGFICRLLCPKYGGYLTFGTLEEGIVSAPGQPTIKDLLNLYNFRHIGPDTKVFGIIGNPVGDSKLPALYNELFKLVGLDAIYVPLLVDDPTKFLDTYSSSDIGGFSVTIPHKEAVFKYCDEVDSVAKATGAVNFVLRRQSDGKLCGYNTDCYGAISAIEDGLLGLHNDSFAVSSRLAGKLVVVIGAGGVGKAVAYGAKQKGARIVIANRSFDRARELADRVGGQSVSLADLENFHPEDGMILVNATPIGMQPKVDETPIPKHALKRYSLVFDAVYTPRMTRLLREAEESGAAIVPGLEMFTRQAYEQYEGFTGLPAPKERIRKFLADN

Target ORF information:

RefSeq Version	XM_004141926.2
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic (LOC101209860), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004141926.2

ORF Insert Sequence:

ATGGAGTTTG GGCCGGCATT TTCTGGCTTA GGCATCAGAA CTGAAGGGAT TAGCAGGAAT 
TCAACACTTA TTTGTGTGCC AATAATGTCA GAATCAATTG ACAAGATGAT TGTTGACGTT 
AAGAAAGCTG GAGAGAATGG TGCTGACATT GTGGAGATAA GATTGGACGC ATTGAAGATT 
TTCAATCCCC ATGAAGATCT TAAACTTCTC ATTAAGGAGT CCCCTCTACC CACCTTATTC 
ACTTACAGAC CAAATTGGGA GGGTGGTCAG TATGATGGTG ATGAAAACCA ACGACTGGAT 
GTTCTTCGAT TAGCCATGAA ATTGGGAGCA GACTATATTG ATGTTGAGCT TCAGGTGGCT 
CGGGAGTTCA TTGACTCTAT ACAGCAAGAG AAGCCAAACA ATTTTAAAGT CATTGTTTCC 
AATCACAATT ATAAGAAGAC CCCATCTTTA GAGGATTTAG GGAGTCTTGT TGCAGAAATC 
CAAGCAACTG GAGCTGACAT TGTGAAGATT GCAACGACTG CATTGGATAT CACTGATGTT 
GCTCGCATGT TTCAAGTACT TGTCTATTCA CAAGTTCCAA TAATTGGACT GGTTATGAAG 
GATAGAGGAT TCATTTGTCG ACTACTTTGC CCCAAATATG GTGGTTATCT TACTTTTGGT 
ACCCTTGAGG AGGGAATAGT TTCAGCTCCT GGTCAACCGA CCATCAAAGA TCTATTAAAT 
CTATACAACT TCAGACACAT AGGGCCTGAC ACTAAGGTCT TTGGCATTAT TGGAAACCCT 
GTAGGTGACA GCAAATTACC TGCATTGTAC AATGAGCTTT TCAAGTTAGT TGGTCTCGAC 
GCCATTTATG TTCCCTTATT GGTGGACGAT CCTACCAAGT TCTTAGATAC TTATTCATCA 
AGTGATATTG GCGGATTCAG TGTTACAATC CCACACAAGG AAGCAGTATT CAAATATTGC 
GATGAGGTTG ATTCAGTTGC AAAGGCAACT GGAGCTGTTA ACTTTGTTCT TAGAAGACAA 
TCTGATGGAA AATTATGTGG CTATAACACA GATTGTTATG GTGCTATTTC AGCCATTGAA 
GACGGATTAC TCGGCTTGCA TAACGACAGC TTCGCAGTCA GTTCACGCTT AGCTGGAAAG 
CTCGTTGTTG TCATTGGTGC TGGTGGAGTT GGCAAAGCTG TTGCTTATGG TGCAAAACAA 
AAGGGAGCCA GGATTGTAAT TGCTAACCGC TCGTTTGATC GGGCTAGAGA ACTTGCAGAC 
CGTGTTGGGG GACAGTCTGT GTCACTTGCT GACTTAGAAA ATTTTCACCC AGAGGATGGC 
ATGATTCTTG TCAACGCTAC GCCAATTGGA ATGCAGCCCA AAGTTGATGA AACTCCCATT 
CCAAAGCATG CTCTAAAACG CTACTCCCTG GTTTTCGATG CTGTTTACAC ACCAAGAATG 
ACTAGACTCT TGCGGGAAGC TGAGGAGTCT GGAGCAGCTA TTGTTCCTGG GTTGGAGATG 
TTCACCAGGC AAGCATATGA ACAATATGAA GGGTTCACCG GCCTACCTGC ACCCAAGGAA 
CGTATTAGGA AATTTTTGGC AGATAATTAA