ITCH cDNA ORF clone, Chaetura pelagica(chimney swift)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITCH
Entrez Gene ID	104390256
Full Name	itchy E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Chaetura pelagica(chimney swift)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009999360.1	XP_009997662.1	E3 ubiquitin-protein ligase Itchy homolog

Pathways from BioSystems
Gene Ontology

The following ITCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCh185812	XM_009999360.1 Latest version!	Chaetura pelagica itchy E3 ubiquitin protein ligase (ITCH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITCH ( XM_009999360.1 ) cDNA ORF clone, Chaetura pelagica(chimney swift) -> XP_009997662.1 Chaetura pelagica itchy E3 ubiquitin protein ligase (ITCH), mRNA.

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CloneID	OCh185812
Clone ID Related Accession (Same CDS sequence)	XM_009999360.1
Accession Version	XM_009999360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Chaetura pelagica(chimney swift)
Product	E3 ubiquitin-protein ligase Itchy homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009953503.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Chaetura pelagica Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGCA GTGGGTCTAA ATCAGGACCA ACTGGTGGGC TTTCCATGAA ATCACAGCTT 
CAGATCACAG TTATTTCAGC AAAACTAAAA GAAAAGAATA AATGGTTTGG ACCTAGCCCT 
TACGTGGAAG TGTCAGTAGA TGGGCAGTCA AAGAAGACAG AAAAATGCAA CAACACAAAT 
AGTCCAAAGT GGAAACAGCA TCTTACAGTA ATTGTCACTC CTGTAAGTAA ACTAAACTTT 
CGAGTCTGGA GCCATCAAAC ATTAAAATCT GATGTCCTTC TGGGAAGTGC TGCTCTGGAT 
ATTCATGAGA CTTTGAAATC AAACAGTATG AAACTTGAGC AGGTAGTGGT GACACTGCAT 
CTTGCTGGTG ACAGAGAACC AACAGAAGTG GTGGGGGATT TATCTGTCTG TCTAGATGGG 
ATGCAGGTTG ATCCTGAGCT CCTCACCAAT GGTGAAGCCT CAGGTACAAG AAGTCCTACA 
CAGAATGACA GCTCGAAAGT AAAGAATGAT ACACGAACTA ACTCAAATGG CCTTGAAAGC 
TGTGAAGATG CTGTTCCCAG TGAAAAAAAG ACTGTTAATG GCAGTGATTC TCCCTTACTC 
ACAAATGGAA ACTGCAAACC TCCTAGACCT CCCAGGCCTT CTAGACCACC TCCACCCACA 
CCCCGCAGAC CCACCTCCAT AGCTGCAAGT GTAAATGGTC CCTCCTCCAC GAGCACTGAA 
AATGATGGAG CCAGTGCAGG ATCACTGGCA GGTACAGCTT CAAACACACC TTCAGAGCAG 
AGCCCTGAGG GGGCAACAGC TGCAACTACA GCTGCAACTA CAGCTGCAAC AACAGCTCCT 
GCAACACCAG TAAACCCTTC ACCTCAGGCC ACAGTCAGTC AAGGTCCTCT TCCACCTGGT 
TGGGAGCAAA GAGTAGACCA ACATGGTCGT GTGTACTATG TAGATCATGT TGAGAAGAGA 
ACAACGTGGG ATCGACCAGA GCCTCTTCCT CCAAGCTGGG AGCGACGAGT TGACAACATG 
GGGAGAATTT ATTACGTTGA CCATTTCACC AGAACCACAA CGTGGCAGAG ACCAACCTTG 
GAGTCAGTGC GGAACTACGA GCAGTGGCAG CTGCAGCGCA GCCAGCTGCA GGGAGCCATG 
CAGCAGTTCA ACCAGAGGTT CATCTATGGG AACCAGGATT TTTCATCCAC ACAGAACAAA 
GAGTTTGATC CTCTTGGCCC TCTGCCGTCT GGATGGGAAA AGAGAACTGA CAGCAATGGC 
AGGGTGTATT TTGTCAATCA CAACACACGA ATTACTCAGT GGGAAGATCC CAGGAGTCAA 
GGTCAATTAA ATGAGAAGCC CTTACCAGAA GGCTGGGAAA TGAGATTCAC TGTGGATGGC 
ATTCCCTATT TTGTGGATCA CAACAGAAGA ACTACCACCT ACATAGACCC CCGCACGGGC 
AAGTCTGCTC TAGACAATGG ACCCCAGATA GCTTATGTGC GTGACTTCAA GGCAAAGGTC 
CATTATTTCA GATTCTGGTG TCAGCAACTG GGGATGCCAC AGCACATAAA GATCACAGTG 
AGCAGAAAAA CATTATTTGA GGACTCATTT CAACAGATAA TGAGCTTCAG CCCTCAGGAT 
CTACGGAGAC GTTTATGGGT TATTTTCCCT GGTGAAGAAG GCTTAGATTA TGGAGGTGTT 
GCAAGGGAGT GGTTCTTTCT ATTGTCACAT GAAGTTTTGA ACCCAATGTA TTGCCTGTTT 
GAATATGCAG GGAAAGATAA CTACTGCTTA CAGATAAACC CAGCCTCTTA CATTAATCCA 
GACCACCTGA AATACTTCCG ATTTATTGGA AGATTCATTG CCATGGCTCT GTTCCATGGG 
AAGTTCATTG ACACTGGTTT CTCTCTGCCC TTCTATAAAC GGATGCTAAA CAAGCCAGTA 
GGACTCAAGG ATTTAGAATC TGTTGACCCA GAGTTTTACA ACTCTCTCAT CTGGGTAAAA 
GAGAATAATA TTGAAGAATG TGGCTTGGAA ATGTTTTTCT CAGTTGATAA AGAAATCCTA 
GGTGAAATCA AAAGCCATGA TTTGAAGCCA AATGGTAGCA ACATTCTCGT CACAGAAGAA 
AACAAGGAAG AGTATATTAG GTTAGTAGCA GAATGGAGAC TTTCCCGAGG TGTTGAGGAG 
CAGACACAGG CTTTCTTTGA AGGCTTCAAT GAAATTCTGC CACAACAGTA TTTGCAGTAT 
TTTGATGCTA AGGAACTGGA GGTGCTTCTG TGTGGAATGC AAGAAATTGA CCTGAACGAC 
TGGCAGAGAC ACACCATCTA CAGGCACTAC ACCAGGACCA GCAGGCAGAT CCTCTGGTTC 
TGGCAGTTTG TAAAAGAAAT AGATAATGAG AAGAGGATGA GACTCTTGCA GTTTGTCACT 
GGAACATGCA GATTACCCGT GGGAGGATTT GTTGACCTCA TGGGGAGCAA CGGCCCCCAG 
AAGTTTTGTA TTGAAAAAGT TGGAAAGGAA AATTGGTTAC CTAGGAGTCA CACCTGTTTC 
AATCGCTTAG ACCTTCCACC CTATAAGAAT TACGAGCAGT TGAAGGAAAA GCTGTTGTTT 
GCAATTGAAG AAACAGAAGG ATTTGGGCAA GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009997662.1
CDS	20..2635
Translation	MAGSGSKSGPTGGLSMKSQLQITVISAKLKEKNKWFGPSPYVEVSVDGQSKKTEKCNNTNSPKWKQHLTVIVTPVSKLNFRVWSHQTLKSDVLLGSAALDIHETLKSNSMKLEQVVVTLHLAGDREPTEVVGDLSVCLDGMQVDPELLTNGEASGTRSPTQNDSSKVKNDTRTNSNGLESCEDAVPSEKKTVNGSDSPLLTNGNCKPPRPPRPSRPPPPTPRRPTSIAASVNGPSSTSTENDGASAGSLAGTASNTPSEQSPEGATAATTAATTAATTAPATPVNPSPQATVSQGPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPSWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDFSSTQNKEFDPLGPLPSGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKSALDNGPQIAYVRDFKAKVHYFRFWCQQLGMPQHIKITVSRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRMLNKPVGLKDLESVDPEFYNSLIWVKENNIEECGLEMFFSVDKEILGEIKSHDLKPNGSNILVTEENKEEYIRLVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHTIYRHYTRTSRQILWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFVDLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKNYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_009999360.1
Organism	Chaetura pelagica(chimney swift)
Definition	Chaetura pelagica itchy E3 ubiquitin protein ligase (ITCH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009999360.1

ORF Insert Sequence:

ATGGCTGGCA GTGGGTCTAA ATCAGGACCA ACTGGTGGGC TTTCCATGAA ATCACAGCTT 
CAGATCACAG TTATTTCAGC AAAACTAAAA GAAAAGAATA AATGGTTTGG ACCTAGCCCT 
TACGTGGAAG TGTCAGTAGA TGGGCAGTCA AAGAAGACAG AAAAATGCAA CAACACAAAT 
AGTCCAAAGT GGAAACAGCA TCTTACAGTA ATTGTCACTC CTGTAAGTAA ACTAAACTTT 
CGAGTCTGGA GCCATCAAAC ATTAAAATCT GATGTCCTTC TGGGAAGTGC TGCTCTGGAT 
ATTCATGAGA CTTTGAAATC AAACAGTATG AAACTTGAGC AGGTAGTGGT GACACTGCAT 
CTTGCTGGTG ACAGAGAACC AACAGAAGTG GTGGGGGATT TATCTGTCTG TCTAGATGGG 
ATGCAGGTTG ATCCTGAGCT CCTCACCAAT GGTGAAGCCT CAGGTACAAG AAGTCCTACA 
CAGAATGACA GCTCGAAAGT AAAGAATGAT ACACGAACTA ACTCAAATGG CCTTGAAAGC 
TGTGAAGATG CTGTTCCCAG TGAAAAAAAG ACTGTTAATG GCAGTGATTC TCCCTTACTC 
ACAAATGGAA ACTGCAAACC TCCTAGACCT CCCAGGCCTT CTAGACCACC TCCACCCACA 
CCCCGCAGAC CCACCTCCAT AGCTGCAAGT GTAAATGGTC CCTCCTCCAC GAGCACTGAA 
AATGATGGAG CCAGTGCAGG ATCACTGGCA GGTACAGCTT CAAACACACC TTCAGAGCAG 
AGCCCTGAGG GGGCAACAGC TGCAACTACA GCTGCAACTA CAGCTGCAAC AACAGCTCCT 
GCAACACCAG TAAACCCTTC ACCTCAGGCC ACAGTCAGTC AAGGTCCTCT TCCACCTGGT 
TGGGAGCAAA GAGTAGACCA ACATGGTCGT GTGTACTATG TAGATCATGT TGAGAAGAGA 
ACAACGTGGG ATCGACCAGA GCCTCTTCCT CCAAGCTGGG AGCGACGAGT TGACAACATG 
GGGAGAATTT ATTACGTTGA CCATTTCACC AGAACCACAA CGTGGCAGAG ACCAACCTTG 
GAGTCAGTGC GGAACTACGA GCAGTGGCAG CTGCAGCGCA GCCAGCTGCA GGGAGCCATG 
CAGCAGTTCA ACCAGAGGTT CATCTATGGG AACCAGGATT TTTCATCCAC ACAGAACAAA 
GAGTTTGATC CTCTTGGCCC TCTGCCGTCT GGATGGGAAA AGAGAACTGA CAGCAATGGC 
AGGGTGTATT TTGTCAATCA CAACACACGA ATTACTCAGT GGGAAGATCC CAGGAGTCAA 
GGTCAATTAA ATGAGAAGCC CTTACCAGAA GGCTGGGAAA TGAGATTCAC TGTGGATGGC 
ATTCCCTATT TTGTGGATCA CAACAGAAGA ACTACCACCT ACATAGACCC CCGCACGGGC 
AAGTCTGCTC TAGACAATGG ACCCCAGATA GCTTATGTGC GTGACTTCAA GGCAAAGGTC 
CATTATTTCA GATTCTGGTG TCAGCAACTG GGGATGCCAC AGCACATAAA GATCACAGTG 
AGCAGAAAAA CATTATTTGA GGACTCATTT CAACAGATAA TGAGCTTCAG CCCTCAGGAT 
CTACGGAGAC GTTTATGGGT TATTTTCCCT GGTGAAGAAG GCTTAGATTA TGGAGGTGTT 
GCAAGGGAGT GGTTCTTTCT ATTGTCACAT GAAGTTTTGA ACCCAATGTA TTGCCTGTTT 
GAATATGCAG GGAAAGATAA CTACTGCTTA CAGATAAACC CAGCCTCTTA CATTAATCCA 
GACCACCTGA AATACTTCCG ATTTATTGGA AGATTCATTG CCATGGCTCT GTTCCATGGG 
AAGTTCATTG ACACTGGTTT CTCTCTGCCC TTCTATAAAC GGATGCTAAA CAAGCCAGTA 
GGACTCAAGG ATTTAGAATC TGTTGACCCA GAGTTTTACA ACTCTCTCAT CTGGGTAAAA 
GAGAATAATA TTGAAGAATG TGGCTTGGAA ATGTTTTTCT CAGTTGATAA AGAAATCCTA 
GGTGAAATCA AAAGCCATGA TTTGAAGCCA AATGGTAGCA ACATTCTCGT CACAGAAGAA 
AACAAGGAAG AGTATATTAG GTTAGTAGCA GAATGGAGAC TTTCCCGAGG TGTTGAGGAG 
CAGACACAGG CTTTCTTTGA AGGCTTCAAT GAAATTCTGC CACAACAGTA TTTGCAGTAT 
TTTGATGCTA AGGAACTGGA GGTGCTTCTG TGTGGAATGC AAGAAATTGA CCTGAACGAC 
TGGCAGAGAC ACACCATCTA CAGGCACTAC ACCAGGACCA GCAGGCAGAT CCTCTGGTTC 
TGGCAGTTTG TAAAAGAAAT AGATAATGAG AAGAGGATGA GACTCTTGCA GTTTGTCACT 
GGAACATGCA GATTACCCGT GGGAGGATTT GTTGACCTCA TGGGGAGCAA CGGCCCCCAG 
AAGTTTTGTA TTGAAAAAGT TGGAAAGGAA AATTGGTTAC CTAGGAGTCA CACCTGTTTC 
AATCGCTTAG ACCTTCCACC CTATAAGAAT TACGAGCAGT TGAAGGAAAA GCTGTTGTTT 
GCAATTGAAG AAACAGAAGG ATTTGGGCAA GAGTAA