LOC100749463 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100749463
Entrez Gene ID	100749463
Full Name	ubiquitin carboxyl-terminal hydrolase 7
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012387504.1	XP_012242927.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X2
XM_012387499.1	XP_012242922.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X1
XM_012387514.1	XP_012242937.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X3
XM_012387504.2	XP_012242927.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X2
XM_012387523.2	XP_012242946.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X4
XM_012387499.2	XP_012242922.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X1
XM_012387514.2	XP_012242937.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X3
XM_012387523.1	XP_012242946.1	ubiquitin carboxyl-terminal hydrolase 7 isoform X4

Pathways from BioSystems
Gene Ontology

The following LOC100749463 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100749463 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo96769	XM_012387499.2 Latest version!	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo96770	XM_012387504.2 Latest version!	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo96771	XM_012387514.2 Latest version!	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo96772	XM_012387523.2 Latest version!	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OBo96770	XM_012387504.1	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo96769	XM_012387499.1	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo96771	XM_012387514.1	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo96772	XM_012387523.1	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100749463 ( XM_012387499.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012242922.1 Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo96769
Clone ID Related Accession (Same CDS sequence)	XM_012387499.1 , XM_012387499.2
Accession Version	XM_012387499.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176452.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTTAAGTTC 
GCGGTGGTGA CGATGGGTAA ACCACACTTT ATTATGGACT CGCCAGAATA CTGCATGGAC 
CTTGCAGATT TCCGTATTCA TCCAAATCAA ATTTATCCAT TTTTAAACGC AGGCACATCG 
CCACTTAGGC CTTGGCTCGG CCTAGAACAC GTCAACAAAG CGCCAAAGCG CTCTCGTATC 
AACTACCTCG AAAAGGCCAT TAAAATTTAC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012242922.1
CDS	1..3336
Translation	MNHVNDQENLKQLNLAPVQVNEVEEMDTQEGETPNDGGGDGNDTSPMNGESELACIVQDQEMEEDEARSEATFRYTVENLSKMKETQLSPPCYVRNLPWKIMIMPRSSQTQDRAPQKSLGFFLQCNGESESSWSCYAVADLRLLSCKEGQEPFSRKIQHLFYSKENDWGFSHFMTWQDVLDPDKGFIKDDSITLEVHVMADAPHGVSWDSKKHTGFVGLKNQGATCYMNSLLQTLYFTNQLRKAVYKMPTESDDSSKSVALALQRVFHELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTCVEGTVPKLFEGKMVSFIKCKNIDYKSTRVETFYDIQLNIKGKKNIYESFNDYVSTESLDGDNKYDAGENGLQEAEKGVIFSSFPPVLHLHLMRFQYDPVTDCSVKFNDRFEFYEKISLGKYLQNKEATSADYTLHAVLVHSGDNHGGHYVVFINPAGDGKWCKFDDDVVSRCTKQEAIEHNYGGQDEDMSIAVKHCTNAYMLVYIRDSELENVLQEVKEEDIPQELVERLQEEKRLEQIRRKERTEAYLYITVNVLLEDNFDGHQGNDLYDPEHALYRVFRVRKQCTLHEFLELLSDSLKYPIEQIRLWPLNVRSNQTCRPMPIELENDLQKSIYQCAENPNVWNVFVELVPPDSDLTALPPFDKDTDVLLFFKLYDPKNKKIHYCGHHYMPVTAKVQELIPILNERAGFPPDTELALYEEIKPNLVEKIDNLTEPLEKVLEELMDGDIIVFQKEGDNQMYELPTCREYFKDLFHRVEVTFCDKTIPNDTGFTMELSLRMTYDQMARAVAQRLGTDPYLLQFFKCQTYKDSPGHPLKCTFEGSLKDLVSYCKPKAKKLYYQQLSIRVNELENKKQFKCIWVGPSLKEEKEIILYPNKNGTVATLLEEAKKQVELSENGSGKLRILEINSSKLSPGPREDVPLDNLNTSGTKLYRIEEIPNDELNLAEDEMLVPVAHFHKDIFSTFGIPFFFKIKHGEPFPKMKERLLKKLGVQEKEFEKFKFAVVTMGKPHFIMDSPEYCMDLADFRIHPNQIYPFLNAGTSPLRPWLGLEHVNKAPKRSRINYLEKAIKIYN

Target ORF information:

RefSeq Version	XM_012387499.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387499.1

ORF Insert Sequence:

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTTAAGTTC 
GCGGTGGTGA CGATGGGTAA ACCACACTTT ATTATGGACT CGCCAGAATA CTGCATGGAC 
CTTGCAGATT TCCGTATTCA TCCAAATCAA ATTTATCCAT TTTTAAACGC AGGCACATCG 
CCACTTAGGC CTTGGCTCGG CCTAGAACAC GTCAACAAAG CGCCAAAGCG CTCTCGTATC 
AACTACCTCG AAAAGGCCAT TAAAATTTAC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749463 ( XM_012387499.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012242922.1 Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo96769
Clone ID Related Accession (Same CDS sequence)	XM_012387499.1 , XM_012387499.2
Accession Version	XM_012387499.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176452.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012387499.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTTAAGTTC 
GCGGTGGTGA CGATGGGTAA ACCACACTTT ATTATGGACT CGCCAGAATA CTGCATGGAC 
CTTGCAGATT TCCGTATTCA TCCAAATCAA ATTTATCCAT TTTTAAACGC AGGCACATCG 
CCACTTAGGC CTTGGCTCGG CCTAGAACAC GTCAACAAAG CGCCAAAGCG CTCTCGTATC 
AACTACCTCG AAAAGGCCAT TAAAATTTAC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012242922.1
CDS	813..4148
Translation	MNHVNDQENLKQLNLAPVQVNEVEEMDTQEGETPNDGGGDGNDTSPMNGESELACIVQDQEMEEDEARSEATFRYTVENLSKMKETQLSPPCYVRNLPWKIMIMPRSSQTQDRAPQKSLGFFLQCNGESESSWSCYAVADLRLLSCKEGQEPFSRKIQHLFYSKENDWGFSHFMTWQDVLDPDKGFIKDDSITLEVHVMADAPHGVSWDSKKHTGFVGLKNQGATCYMNSLLQTLYFTNQLRKAVYKMPTESDDSSKSVALALQRVFHELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTCVEGTVPKLFEGKMVSFIKCKNIDYKSTRVETFYDIQLNIKGKKNIYESFNDYVSTESLDGDNKYDAGENGLQEAEKGVIFSSFPPVLHLHLMRFQYDPVTDCSVKFNDRFEFYEKISLGKYLQNKEATSADYTLHAVLVHSGDNHGGHYVVFINPAGDGKWCKFDDDVVSRCTKQEAIEHNYGGQDEDMSIAVKHCTNAYMLVYIRDSELENVLQEVKEEDIPQELVERLQEEKRLEQIRRKERTEAYLYITVNVLLEDNFDGHQGNDLYDPEHALYRVFRVRKQCTLHEFLELLSDSLKYPIEQIRLWPLNVRSNQTCRPMPIELENDLQKSIYQCAENPNVWNVFVELVPPDSDLTALPPFDKDTDVLLFFKLYDPKNKKIHYCGHHYMPVTAKVQELIPILNERAGFPPDTELALYEEIKPNLVEKIDNLTEPLEKVLEELMDGDIIVFQKEGDNQMYELPTCREYFKDLFHRVEVTFCDKTIPNDTGFTMELSLRMTYDQMARAVAQRLGTDPYLLQFFKCQTYKDSPGHPLKCTFEGSLKDLVSYCKPKAKKLYYQQLSIRVNELENKKQFKCIWVGPSLKEEKEIILYPNKNGTVATLLEEAKKQVELSENGSGKLRILEINSSKLSPGPREDVPLDNLNTSGTKLYRIEEIPNDELNLAEDEMLVPVAHFHKDIFSTFGIPFFFKIKHGEPFPKMKERLLKKLGVQEKEFEKFKFAVVTMGKPHFIMDSPEYCMDLADFRIHPNQIYPFLNAGTSPLRPWLGLEHVNKAPKRSRINYLEKAIKIYN

Target ORF information:

RefSeq Version	XM_012387499.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387499.2

ORF Insert Sequence:

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTTAAGTTC 
GCGGTGGTGA CGATGGGTAA ACCACACTTT ATTATGGACT CGCCAGAATA CTGCATGGAC 
CTTGCAGATT TCCGTATTCA TCCAAATCAA ATTTATCCAT TTTTAAACGC AGGCACATCG 
CCACTTAGGC CTTGGCTCGG CCTAGAACAC GTCAACAAAG CGCCAAAGCG CTCTCGTATC 
AACTACCTCG AAAAGGCCAT TAAAATTTAC AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749463 ( XM_012387504.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012242927.1 Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo96770
Clone ID Related Accession (Same CDS sequence)	XM_012387504.1 , XM_012387504.2
Accession Version	XM_012387504.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176452.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTCGCGGTG 
GTGACGATGG GTAAACCACA CTTTATTATG GACTCGCCAG AATACTGCAT GGACCTTGCA 
GATTTCCGTA TTCATCCAAA TCAAATTTAT CCATTTTTAA ACGCAGGCAC ATCGCCACTT 
AGGCCTTGGC TCGGCCTAGA ACACGTCAAC AAAGCGCCAA AGCGCTCTCG TATCAACTAC 
CTCGAAAAGG CCATTAAAAT TTACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012242927.1
CDS	817..4146
Translation	MNHVNDQENLKQLNLAPVQVNEVEEMDTQEGETPNDGGGDGNDTSPMNGESELACIVQDQEMEEDEARSEATFRYTVENLSKMKETQLSPPCYVRNLPWKIMIMPRSSQTQDRAPQKSLGFFLQCNGESESSWSCYAVADLRLLSCKEGQEPFSRKIQHLFYSKENDWGFSHFMTWQDVLDPDKGFIKDDSITLEVHVMADAPHGVSWDSKKHTGFVGLKNQGATCYMNSLLQTLYFTNQLRKAVYKMPTESDDSSKSVALALQRVFHELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTCVEGTVPKLFEGKMVSFIKCKNIDYKSTRVETFYDIQLNIKGKKNIYESFNDYVSTESLDGDNKYDAGENGLQEAEKGVIFSSFPPVLHLHLMRFQYDPVTDCSVKFNDRFEFYEKISLGKYLQNKEATSADYTLHAVLVHSGDNHGGHYVVFINPAGDGKWCKFDDDVVSRCTKQEAIEHNYGGQDEDMSIAVKHCTNAYMLVYIRDSELENVLQEVKEEDIPQELVERLQEEKRLEQIRRKERTEAYLYITVNVLLEDNFDGHQGNDLYDPEHALYRVFRVRKQCTLHEFLELLSDSLKYPIEQIRLWPLNVRSNQTCRPMPIELENDLQKSIYQCAENPNVWNVFVELVPPDSDLTALPPFDKDTDVLLFFKLYDPKNKKIHYCGHHYMPVTAKVQELIPILNERAGFPPDTELALYEEIKPNLVEKIDNLTEPLEKVLEELMDGDIIVFQKEGDNQMYELPTCREYFKDLFHRVEVTFCDKTIPNDTGFTMELSLRMTYDQMARAVAQRLGTDPYLLQFFKCQTYKDSPGHPLKCTFEGSLKDLVSYCKPKAKKLYYQQLSIRVNELENKKQFKCIWVGPSLKEEKEIILYPNKNGTVATLLEEAKKQVELSENGSGKLRILEINSSKLSPGPREDVPLDNLNTSGTKLYRIEEIPNDELNLAEDEMLVPVAHFHKDIFSTFGIPFFFKIKHGEPFPKMKERLLKKLGVQEKEFEKFAVVTMGKPHFIMDSPEYCMDLADFRIHPNQIYPFLNAGTSPLRPWLGLEHVNKAPKRSRINYLEKAIKIYN

Target ORF information:

RefSeq Version	XM_012387504.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387504.1

ORF Insert Sequence:

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTCGCGGTG 
GTGACGATGG GTAAACCACA CTTTATTATG GACTCGCCAG AATACTGCAT GGACCTTGCA 
GATTTCCGTA TTCATCCAAA TCAAATTTAT CCATTTTTAA ACGCAGGCAC ATCGCCACTT 
AGGCCTTGGC TCGGCCTAGA ACACGTCAAC AAAGCGCCAA AGCGCTCTCG TATCAACTAC 
CTCGAAAAGG CCATTAAAAT TTACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749463 ( XM_012387504.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012242927.1 Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo96770
Clone ID Related Accession (Same CDS sequence)	XM_012387504.1 , XM_012387504.2
Accession Version	XM_012387504.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176452.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012387504.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTCGCGGTG 
GTGACGATGG GTAAACCACA CTTTATTATG GACTCGCCAG AATACTGCAT GGACCTTGCA 
GATTTCCGTA TTCATCCAAA TCAAATTTAT CCATTTTTAA ACGCAGGCAC ATCGCCACTT 
AGGCCTTGGC TCGGCCTAGA ACACGTCAAC AAAGCGCCAA AGCGCTCTCG TATCAACTAC 
CTCGAAAAGG CCATTAAAAT TTACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012242927.1
CDS	813..4142
Translation	MNHVNDQENLKQLNLAPVQVNEVEEMDTQEGETPNDGGGDGNDTSPMNGESELACIVQDQEMEEDEARSEATFRYTVENLSKMKETQLSPPCYVRNLPWKIMIMPRSSQTQDRAPQKSLGFFLQCNGESESSWSCYAVADLRLLSCKEGQEPFSRKIQHLFYSKENDWGFSHFMTWQDVLDPDKGFIKDDSITLEVHVMADAPHGVSWDSKKHTGFVGLKNQGATCYMNSLLQTLYFTNQLRKAVYKMPTESDDSSKSVALALQRVFHELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTCVEGTVPKLFEGKMVSFIKCKNIDYKSTRVETFYDIQLNIKGKKNIYESFNDYVSTESLDGDNKYDAGENGLQEAEKGVIFSSFPPVLHLHLMRFQYDPVTDCSVKFNDRFEFYEKISLGKYLQNKEATSADYTLHAVLVHSGDNHGGHYVVFINPAGDGKWCKFDDDVVSRCTKQEAIEHNYGGQDEDMSIAVKHCTNAYMLVYIRDSELENVLQEVKEEDIPQELVERLQEEKRLEQIRRKERTEAYLYITVNVLLEDNFDGHQGNDLYDPEHALYRVFRVRKQCTLHEFLELLSDSLKYPIEQIRLWPLNVRSNQTCRPMPIELENDLQKSIYQCAENPNVWNVFVELVPPDSDLTALPPFDKDTDVLLFFKLYDPKNKKIHYCGHHYMPVTAKVQELIPILNERAGFPPDTELALYEEIKPNLVEKIDNLTEPLEKVLEELMDGDIIVFQKEGDNQMYELPTCREYFKDLFHRVEVTFCDKTIPNDTGFTMELSLRMTYDQMARAVAQRLGTDPYLLQFFKCQTYKDSPGHPLKCTFEGSLKDLVSYCKPKAKKLYYQQLSIRVNELENKKQFKCIWVGPSLKEEKEIILYPNKNGTVATLLEEAKKQVELSENGSGKLRILEINSSKLSPGPREDVPLDNLNTSGTKLYRIEEIPNDELNLAEDEMLVPVAHFHKDIFSTFGIPFFFKIKHGEPFPKMKERLLKKLGVQEKEFEKFAVVTMGKPHFIMDSPEYCMDLADFRIHPNQIYPFLNAGTSPLRPWLGLEHVNKAPKRSRINYLEKAIKIYN

Target ORF information:

RefSeq Version	XM_012387504.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387504.2

ORF Insert Sequence:

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTCGCGGTG 
GTGACGATGG GTAAACCACA CTTTATTATG GACTCGCCAG AATACTGCAT GGACCTTGCA 
GATTTCCGTA TTCATCCAAA TCAAATTTAT CCATTTTTAA ACGCAGGCAC ATCGCCACTT 
AGGCCTTGGC TCGGCCTAGA ACACGTCAAC AAAGCGCCAA AGCGCTCTCG TATCAACTAC 
CTCGAAAAGG CCATTAAAAT TTACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749463 ( XM_012387514.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012242937.1 Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X3, mRNA.

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CloneID	OBo96771
Clone ID Related Accession (Same CDS sequence)	XM_012387514.1 , XM_012387514.2
Accession Version	XM_012387514.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176452.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTTAAGTTC 
GCGGTGGTGA CGATGGGTAA ACCACACTTT ATTATGGACT CGCCAGAATA CTGCATGGAC 
CTTGCAGATT TCCGTATTCA TCCAAATCAA AGCACATCGC CACTTAGGCC TTGGCTCGGC 
CTAGAACACG TCAACAAAGC GCCAAAGCGC TCTCGTATCA ACTACCTCGA AAAGGCCATT 
AAAATTTACA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012242937.1
CDS	1..3315
Translation	MNHVNDQENLKQLNLAPVQVNEVEEMDTQEGETPNDGGGDGNDTSPMNGESELACIVQDQEMEEDEARSEATFRYTVENLSKMKETQLSPPCYVRNLPWKIMIMPRSSQTQDRAPQKSLGFFLQCNGESESSWSCYAVADLRLLSCKEGQEPFSRKIQHLFYSKENDWGFSHFMTWQDVLDPDKGFIKDDSITLEVHVMADAPHGVSWDSKKHTGFVGLKNQGATCYMNSLLQTLYFTNQLRKAVYKMPTESDDSSKSVALALQRVFHELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTCVEGTVPKLFEGKMVSFIKCKNIDYKSTRVETFYDIQLNIKGKKNIYESFNDYVSTESLDGDNKYDAGENGLQEAEKGVIFSSFPPVLHLHLMRFQYDPVTDCSVKFNDRFEFYEKISLGKYLQNKEATSADYTLHAVLVHSGDNHGGHYVVFINPAGDGKWCKFDDDVVSRCTKQEAIEHNYGGQDEDMSIAVKHCTNAYMLVYIRDSELENVLQEVKEEDIPQELVERLQEEKRLEQIRRKERTEAYLYITVNVLLEDNFDGHQGNDLYDPEHALYRVFRVRKQCTLHEFLELLSDSLKYPIEQIRLWPLNVRSNQTCRPMPIELENDLQKSIYQCAENPNVWNVFVELVPPDSDLTALPPFDKDTDVLLFFKLYDPKNKKIHYCGHHYMPVTAKVQELIPILNERAGFPPDTELALYEEIKPNLVEKIDNLTEPLEKVLEELMDGDIIVFQKEGDNQMYELPTCREYFKDLFHRVEVTFCDKTIPNDTGFTMELSLRMTYDQMARAVAQRLGTDPYLLQFFKCQTYKDSPGHPLKCTFEGSLKDLVSYCKPKAKKLYYQQLSIRVNELENKKQFKCIWVGPSLKEEKEIILYPNKNGTVATLLEEAKKQVELSENGSGKLRILEINSSKLSPGPREDVPLDNLNTSGTKLYRIEEIPNDELNLAEDEMLVPVAHFHKDIFSTFGIPFFFKIKHGEPFPKMKERLLKKLGVQEKEFEKFKFAVVTMGKPHFIMDSPEYCMDLADFRIHPNQSTSPLRPWLGLEHVNKAPKRSRINYLEKAIKIYN

Target ORF information:

RefSeq Version	XM_012387514.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387514.1

ORF Insert Sequence:

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTTAAGTTC 
GCGGTGGTGA CGATGGGTAA ACCACACTTT ATTATGGACT CGCCAGAATA CTGCATGGAC 
CTTGCAGATT TCCGTATTCA TCCAAATCAA AGCACATCGC CACTTAGGCC TTGGCTCGGC 
CTAGAACACG TCAACAAAGC GCCAAAGCGC TCTCGTATCA ACTACCTCGA AAAGGCCATT 
AAAATTTACA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749463 ( XM_012387514.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012242937.1 Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBo96771
Clone ID Related Accession (Same CDS sequence)	XM_012387514.1 , XM_012387514.2
Accession Version	XM_012387514.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176452.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012387514.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTTAAGTTC 
GCGGTGGTGA CGATGGGTAA ACCACACTTT ATTATGGACT CGCCAGAATA CTGCATGGAC 
CTTGCAGATT TCCGTATTCA TCCAAATCAA AGCACATCGC CACTTAGGCC TTGGCTCGGC 
CTAGAACACG TCAACAAAGC GCCAAAGCGC TCTCGTATCA ACTACCTCGA AAAGGCCATT 
AAAATTTACA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012242937.1
CDS	812..4126
Translation	MNHVNDQENLKQLNLAPVQVNEVEEMDTQEGETPNDGGGDGNDTSPMNGESELACIVQDQEMEEDEARSEATFRYTVENLSKMKETQLSPPCYVRNLPWKIMIMPRSSQTQDRAPQKSLGFFLQCNGESESSWSCYAVADLRLLSCKEGQEPFSRKIQHLFYSKENDWGFSHFMTWQDVLDPDKGFIKDDSITLEVHVMADAPHGVSWDSKKHTGFVGLKNQGATCYMNSLLQTLYFTNQLRKAVYKMPTESDDSSKSVALALQRVFHELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTCVEGTVPKLFEGKMVSFIKCKNIDYKSTRVETFYDIQLNIKGKKNIYESFNDYVSTESLDGDNKYDAGENGLQEAEKGVIFSSFPPVLHLHLMRFQYDPVTDCSVKFNDRFEFYEKISLGKYLQNKEATSADYTLHAVLVHSGDNHGGHYVVFINPAGDGKWCKFDDDVVSRCTKQEAIEHNYGGQDEDMSIAVKHCTNAYMLVYIRDSELENVLQEVKEEDIPQELVERLQEEKRLEQIRRKERTEAYLYITVNVLLEDNFDGHQGNDLYDPEHALYRVFRVRKQCTLHEFLELLSDSLKYPIEQIRLWPLNVRSNQTCRPMPIELENDLQKSIYQCAENPNVWNVFVELVPPDSDLTALPPFDKDTDVLLFFKLYDPKNKKIHYCGHHYMPVTAKVQELIPILNERAGFPPDTELALYEEIKPNLVEKIDNLTEPLEKVLEELMDGDIIVFQKEGDNQMYELPTCREYFKDLFHRVEVTFCDKTIPNDTGFTMELSLRMTYDQMARAVAQRLGTDPYLLQFFKCQTYKDSPGHPLKCTFEGSLKDLVSYCKPKAKKLYYQQLSIRVNELENKKQFKCIWVGPSLKEEKEIILYPNKNGTVATLLEEAKKQVELSENGSGKLRILEINSSKLSPGPREDVPLDNLNTSGTKLYRIEEIPNDELNLAEDEMLVPVAHFHKDIFSTFGIPFFFKIKHGEPFPKMKERLLKKLGVQEKEFEKFKFAVVTMGKPHFIMDSPEYCMDLADFRIHPNQSTSPLRPWLGLEHVNKAPKRSRINYLEKAIKIYN

Target ORF information:

RefSeq Version	XM_012387514.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387514.2

ORF Insert Sequence:

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTTAAGTTC 
GCGGTGGTGA CGATGGGTAA ACCACACTTT ATTATGGACT CGCCAGAATA CTGCATGGAC 
CTTGCAGATT TCCGTATTCA TCCAAATCAA AGCACATCGC CACTTAGGCC TTGGCTCGGC 
CTAGAACACG TCAACAAAGC GCCAAAGCGC TCTCGTATCA ACTACCTCGA AAAGGCCATT 
AAAATTTACA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749463 ( XM_012387523.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012242946.1 Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBo96772
Clone ID Related Accession (Same CDS sequence)	XM_012387523.2 , XM_012387523.1
Accession Version	XM_012387523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176452.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012387523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTCGCGGTG 
GTGACGATGG GTAAACCACA CTTTATTATG GACTCGCCAG AATACTGCAT GGACCTTGCA 
GATTTCCGTA TTCATCCAAA TCAAAGCACA TCGCCACTTA GGCCTTGGCT CGGCCTAGAA 
CACGTCAACA AAGCGCCAAA GCGCTCTCGT ATCAACTACC TCGAAAAGGC CATTAAAATT 
TACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012242946.1
CDS	812..4120
Translation	MNHVNDQENLKQLNLAPVQVNEVEEMDTQEGETPNDGGGDGNDTSPMNGESELACIVQDQEMEEDEARSEATFRYTVENLSKMKETQLSPPCYVRNLPWKIMIMPRSSQTQDRAPQKSLGFFLQCNGESESSWSCYAVADLRLLSCKEGQEPFSRKIQHLFYSKENDWGFSHFMTWQDVLDPDKGFIKDDSITLEVHVMADAPHGVSWDSKKHTGFVGLKNQGATCYMNSLLQTLYFTNQLRKAVYKMPTESDDSSKSVALALQRVFHELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTCVEGTVPKLFEGKMVSFIKCKNIDYKSTRVETFYDIQLNIKGKKNIYESFNDYVSTESLDGDNKYDAGENGLQEAEKGVIFSSFPPVLHLHLMRFQYDPVTDCSVKFNDRFEFYEKISLGKYLQNKEATSADYTLHAVLVHSGDNHGGHYVVFINPAGDGKWCKFDDDVVSRCTKQEAIEHNYGGQDEDMSIAVKHCTNAYMLVYIRDSELENVLQEVKEEDIPQELVERLQEEKRLEQIRRKERTEAYLYITVNVLLEDNFDGHQGNDLYDPEHALYRVFRVRKQCTLHEFLELLSDSLKYPIEQIRLWPLNVRSNQTCRPMPIELENDLQKSIYQCAENPNVWNVFVELVPPDSDLTALPPFDKDTDVLLFFKLYDPKNKKIHYCGHHYMPVTAKVQELIPILNERAGFPPDTELALYEEIKPNLVEKIDNLTEPLEKVLEELMDGDIIVFQKEGDNQMYELPTCREYFKDLFHRVEVTFCDKTIPNDTGFTMELSLRMTYDQMARAVAQRLGTDPYLLQFFKCQTYKDSPGHPLKCTFEGSLKDLVSYCKPKAKKLYYQQLSIRVNELENKKQFKCIWVGPSLKEEKEIILYPNKNGTVATLLEEAKKQVELSENGSGKLRILEINSSKLSPGPREDVPLDNLNTSGTKLYRIEEIPNDELNLAEDEMLVPVAHFHKDIFSTFGIPFFFKIKHGEPFPKMKERLLKKLGVQEKEFEKFAVVTMGKPHFIMDSPEYCMDLADFRIHPNQSTSPLRPWLGLEHVNKAPKRSRINYLEKAIKIYN

Target ORF information:

RefSeq Version	XM_012387523.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387523.2

ORF Insert Sequence:

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTCGCGGTG 
GTGACGATGG GTAAACCACA CTTTATTATG GACTCGCCAG AATACTGCAT GGACCTTGCA 
GATTTCCGTA TTCATCCAAA TCAAAGCACA TCGCCACTTA GGCCTTGGCT CGGCCTAGAA 
CACGTCAACA AAGCGCCAAA GCGCTCTCGT ATCAACTACC TCGAAAAGGC CATTAAAATT 
TACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100749463 ( XM_012387523.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012242946.1 Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X4, mRNA.

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CloneID	OBo96772
Clone ID Related Accession (Same CDS sequence)	XM_012387523.2 , XM_012387523.1
Accession Version	XM_012387523.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3309bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	ubiquitin carboxyl-terminal hydrolase 7 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176452.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTCGCGGTG 
GTGACGATGG GTAAACCACA CTTTATTATG GACTCGCCAG AATACTGCAT GGACCTTGCA 
GATTTCCGTA TTCATCCAAA TCAAAGCACA TCGCCACTTA GGCCTTGGCT CGGCCTAGAA 
CACGTCAACA AAGCGCCAAA GCGCTCTCGT ATCAACTACC TCGAAAAGGC CATTAAAATT 
TACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012242946.1
CDS	817..4125
Translation	MNHVNDQENLKQLNLAPVQVNEVEEMDTQEGETPNDGGGDGNDTSPMNGESELACIVQDQEMEEDEARSEATFRYTVENLSKMKETQLSPPCYVRNLPWKIMIMPRSSQTQDRAPQKSLGFFLQCNGESESSWSCYAVADLRLLSCKEGQEPFSRKIQHLFYSKENDWGFSHFMTWQDVLDPDKGFIKDDSITLEVHVMADAPHGVSWDSKKHTGFVGLKNQGATCYMNSLLQTLYFTNQLRKAVYKMPTESDDSSKSVALALQRVFHELQFSDKPVGTKKLTKSFGWETLDSFMQHDVQEFLRVLLDKLESKMKGTCVEGTVPKLFEGKMVSFIKCKNIDYKSTRVETFYDIQLNIKGKKNIYESFNDYVSTESLDGDNKYDAGENGLQEAEKGVIFSSFPPVLHLHLMRFQYDPVTDCSVKFNDRFEFYEKISLGKYLQNKEATSADYTLHAVLVHSGDNHGGHYVVFINPAGDGKWCKFDDDVVSRCTKQEAIEHNYGGQDEDMSIAVKHCTNAYMLVYIRDSELENVLQEVKEEDIPQELVERLQEEKRLEQIRRKERTEAYLYITVNVLLEDNFDGHQGNDLYDPEHALYRVFRVRKQCTLHEFLELLSDSLKYPIEQIRLWPLNVRSNQTCRPMPIELENDLQKSIYQCAENPNVWNVFVELVPPDSDLTALPPFDKDTDVLLFFKLYDPKNKKIHYCGHHYMPVTAKVQELIPILNERAGFPPDTELALYEEIKPNLVEKIDNLTEPLEKVLEELMDGDIIVFQKEGDNQMYELPTCREYFKDLFHRVEVTFCDKTIPNDTGFTMELSLRMTYDQMARAVAQRLGTDPYLLQFFKCQTYKDSPGHPLKCTFEGSLKDLVSYCKPKAKKLYYQQLSIRVNELENKKQFKCIWVGPSLKEEKEIILYPNKNGTVATLLEEAKKQVELSENGSGKLRILEINSSKLSPGPREDVPLDNLNTSGTKLYRIEEIPNDELNLAEDEMLVPVAHFHKDIFSTFGIPFFFKIKHGEPFPKMKERLLKKLGVQEKEFEKFAVVTMGKPHFIMDSPEYCMDLADFRIHPNQSTSPLRPWLGLEHVNKAPKRSRINYLEKAIKIYN

Target ORF information:

RefSeq Version	XM_012387523.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens ubiquitin carboxyl-terminal hydrolase 7 (LOC100749463), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012387523.1

ORF Insert Sequence:

ATGAACCACG TTAACGATCA GGAAAATCTT AAACAGCTCA ACCTGGCACC GGTTCAGGTC 
AATGAAGTCG AAGAAATGGA TACACAAGAA GGAGAAACAC CAAATGATGG TGGAGGAGAT 
GGTAATGATA CTAGTCCTAT GAACGGAGAA TCAGAATTAG CTTGTATAGT TCAAGATCAA 
GAGATGGAGG AAGATGAAGC AAGATCAGAG GCAACATTTC GTTATACAGT GGAAAATCTT 
TCTAAAATGA AAGAAACCCA GTTGTCACCT CCATGTTACG TTCGTAATTT ACCATGGAAA 
ATAATGATAA TGCCAAGGTC AAGTCAGACA CAAGATAGAG CTCCTCAGAA ATCTCTTGGT 
TTTTTTCTGC AATGTAATGG AGAAAGTGAA TCATCATGGA GTTGTTATGC AGTTGCAGAT 
CTTCGATTAC TTTCTTGTAA GGAAGGACAG GAACCATTTA GTAGAAAAAT TCAACATCTC 
TTCTATAGTA AAGAAAATGA TTGGGGATTT AGTCATTTTA TGACATGGCA GGATGTCCTG 
GATCCTGATA AAGGTTTTAT TAAAGATGAT TCTATTACAC TTGAGGTTCA TGTCATGGCT 
GATGCTCCAC ATGGTGTCAG CTGGGATAGT AAAAAGCATA CAGGATTTGT AGGCTTAAAA 
AATCAAGGTG CTACATGCTA TATGAACTCT TTACTTCAAA CTTTGTATTT TACGAATCAG 
TTACGTAAAG CAGTTTACAA AATGCCAACA GAAAGTGATG ATTCTAGCAA AAGTGTTGCT 
TTGGCTTTAC AAAGGGTATT TCATGAATTA CAGTTCTCTG ATAAGCCAGT AGGTACAAAA 
AAATTAACCA AAAGCTTTGG TTGGGAAACA TTGGATTCAT TCATGCAACA TGATGTACAA 
GAATTTTTAC GAGTGCTTTT AGACAAGTTG GAAAGTAAAA TGAAAGGTAC ATGTGTAGAA 
GGTACAGTGC CAAAATTATT TGAAGGAAAA ATGGTCTCAT TTATAAAATG TAAAAATATT 
GATTACAAAT CAACTAGAGT TGAAACTTTC TATGATATAC AGTTAAATAT AAAAGGAAAG 
AAAAATATTT ATGAGTCTTT TAATGATTAT GTGAGTACTG AAAGTCTGGA TGGTGATAAT 
AAATATGATG CGGGGGAGAA TGGATTGCAA GAGGCAGAGA AAGGTGTTAT CTTTTCATCA 
TTTCCACCAG TGTTGCATTT ACATTTAATG CGATTTCAAT ATGATCCAGT TACAGATTGT 
TCTGTAAAAT TTAATGATAG GTTTGAATTC TATGAAAAAA TAAGCCTTGG CAAATATTTA 
CAAAATAAAG AAGCAACAAG TGCAGATTAT ACATTGCACG CAGTCTTAGT ACACAGTGGG 
GATAATCATG GTGGGCATTA TGTTGTATTT ATCAACCCAG CTGGTGATGG AAAATGGTGC 
AAATTCGACG ATGATGTCGT CTCAAGGTGT ACGAAACAAG AAGCTATTGA ACACAATTAT 
GGTGGTCAAG ACGAGGATAT GTCCATAGCT GTCAAACATT GTACGAACGC CTATATGTTG 
GTGTACATAA GGGACTCTGA GCTGGAAAAC GTCCTACAAG AAGTCAAGGA AGAGGATATA 
CCTCAAGAGC TGGTGGAGAG GCTGCAAGAG GAGAAGAGGC TGGAACAAAT ACGAAGAAAG 
GAAAGAACAG AAGCATACTT GTATATAACC GTCAACGTCC TTCTTGAGGA TAATTTTGAC 
GGTCACCAAG GGAATGACTT ATATGATCCA GAGCATGCTT TGTATCGTGT ATTTCGCGTA 
CGTAAGCAGT GTACCTTGCA CGAGTTTCTC GAATTGCTGA GTGATAGCTT GAAATATCCA 
ATAGAACAAA TTCGTTTGTG GCCGTTGAAT GTGCGTTCAA ACCAGACTTG TAGACCAATG 
CCAATTGAAT TAGAAAATGA CCTACAAAAA TCCATTTACC AATGCGCAGA GAACCCAAAT 
GTTTGGAACG TGTTTGTTGA ACTTGTTCCT CCAGATTCTG ATCTAACAGC ATTGCCACCT 
TTCGATAAAG ACACTGATGT TCTGCTATTT TTTAAATTAT ATGATCCCAA AAATAAGAAG 
ATTCATTACT GCGGACATCA TTATATGCCT GTCACAGCTA AAGTCCAGGA ACTTATACCA 
ATTTTAAATG AAAGAGCCGG TTTCCCACCT GATACAGAAT TGGCTCTTTA CGAAGAAATC 
AAACCAAACT TGGTAGAAAA GATAGACAAC TTAACAGAAC CATTAGAGAA AGTTCTTGAG 
GAATTAATGG ATGGAGATAT AATTGTTTTT CAAAAAGAGG GTGACAATCA AATGTATGAG 
CTTCCAACAT GTAGAGAATA CTTCAAAGAC CTATTTCATA GAGTGGAAGT TACATTTTGT 
GATAAAACAA TTCCTAATGA TACTGGTTTT ACAATGGAAC TTTCATTAAG AATGACATAT 
GACCAAATGG CAAGAGCTGT GGCACAGAGA CTTGGTACAG ATCCTTATCT TTTGCAATTC 
TTTAAATGTC AAACCTACAA AGATTCACCT GGTCACCCAC TAAAATGCAC ATTTGAAGGT 
TCGTTGAAAG ATTTAGTTTC TTATTGTAAA CCTAAAGCAA AAAAGTTATA TTATCAGCAA 
CTTAGTATTA GAGTAAATGA ACTCGAAAAC AAAAAGCAGT TCAAATGTAT ATGGGTTGGT 
CCGTCTCTTA AAGAAGAGAA GGAAATTATT CTTTATCCTA ACAAAAATGG AACAGTAGCA 
ACACTGCTGG AAGAAGCTAA AAAACAAGTA GAATTATCAG AAAACGGATC TGGAAAATTA 
AGGATATTAG AAATTAATTC TAGTAAACTT TCGCCTGGAC CAAGGGAAGA TGTACCTTTA 
GATAATTTAA ATACATCTGG CACAAAATTA TACAGGATAG AAGAAATTCC AAATGATGAG 
TTAAATTTAG CAGAAGATGA AATGTTGGTT CCTGTTGCCC ATTTCCACAA GGATATTTTT 
TCAACATTTG GTATTCCCTT TTTCTTTAAA ATTAAACATG GCGAGCCTTT TCCAAAGATG 
AAAGAAAGAC TATTGAAGAA GTTGGGGGTA CAAGAAAAAG AATTTGAAAA GTTCGCGGTG 
GTGACGATGG GTAAACCACA CTTTATTATG GACTCGCCAG AATACTGCAT GGACCTTGCA 
GATTTCCGTA TTCATCCAAA TCAAAGCACA TCGCCACTTA GGCCTTGGCT CGGCCTAGAA 
CACGTCAACA AAGCGCCAAA GCGCTCTCGT ATCAACTACC TCGAAAAGGC CATTAAAATT 
TACAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100749463 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム