LOC105221203 gene cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

mRNA	Protein	Name
XM_029046294.1	XP_028902127.1	uncharacterized protein LOC105221203 isoform X1
XM_029046296.1	XP_028902129.1	uncharacterized protein LOC105221203 isoform X1
XM_011197975.1	XP_011196277.1	uncharacterized protein LOC105221203 isoform X2
XM_011197973.1	XP_011196275.1	uncharacterized protein LOC105221203 isoform X1
XM_029046297.1	XP_028902130.1	uncharacterized protein LOC105221203 isoform X1
XM_029046298.1	XP_028902131.1	uncharacterized protein LOC105221203 isoform X1
XM_011197973.2	XP_011196275.1	uncharacterized protein LOC105221203 isoform X2
XM_029046295.1	XP_028902128.1	uncharacterized protein LOC105221203 isoform X1
XM_011197974.1	XP_011196276.1	uncharacterized protein LOC105221203 isoform X1
XM_029046299.1	XP_028902132.1	uncharacterized protein LOC105221203 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa193403	XM_011197973.2 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa193403	XM_011197974.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa193404	XM_011197975.1 Latest version!	Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa254280	XM_029046296.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa254280	XM_029046299.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa254280	XM_029046298.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa254280	XM_029046294.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa254280	XM_029046297.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa254280	XM_029046295.1 Latest version!	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
View Old Accession Versions >>
OBa193403	XM_011197973.1	Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

LOC105221203 ( XM_011197973.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196275.1 Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa193403
Clone ID Related Accession (Same CDS sequence)	XM_011197973.1 , XM_011197973.2 , XM_011197974.1
Accession Version	XM_011197973.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT GATGATAGAC AAATGGATTT CGCCGAACAA 
CTCGAGTCTG TGGCATATGT GATTTGTGGC GAAAATAGTA AGATCACACC GAAAAACTTT 
ACAGAAATTT GGCAAGCGCG TGGTATTCTG GATAAATTAT ACCGTCTGAT CGATGTCGAT 
GGCACAAATC TGATATCCAC AAATCAAATA ATGGAATTCA TTTCACACCT GACCAATTCA 
CGGCCACGCA CCGGTTTTGA TAAGAGCTCC CTGGTGCGTC TGGAGCAACT ATTTCGCAGC 
ACTGTAGGCA ATGAACGGGA GATTCGACGC GAAGAATTCC AGAAGATAGT CACATCGAAA 
AACCCATTCT TTACCGATCG TGTCTTTCAA ATTTTCGACA AGGATAACTC CGGTTCGATA 
TCATTGCAAG AGTTCATTGA TGCCATACAT CAATTCTCCG GCCAGTCAGC GGATGATAAA 
ATACGTTTCC TATTCAAAGT ATACGATATC GATGGTGACG GGCTGATACA GCATAAAGAA 
TTGCATGATG TGATACGCGC CTGCATGGAT GAAAATGGTA TGGAATTCTC CGAAGAACAA 
ATTGAAGATC TGACAACGGC TATGTTTGAA GATGCGGATC CATATAATCG TGGTGAAATC 
ACTTATGAAG CCTTAAAAAA TCAAATATGC AAACATGGTG GATTATTAGA GAATCTCAGT 
ATAACCATAG ATCGTTGGCT TGTGCCCATG ACGCAGGAGA ATCCGAAACC AAAGTCGAAA 
TGCCCGCGAT TGCGCTGTGG CAAGCCACAT CAACTCACTT TGGCCTACAT GAAAAACAAT 
CAAGCTTTCG TTTCGTATCT CTACATCTAC ATTGCCATAA ATGTGTGCCT CTTCATCTCA 
CGAGCCATTC AATATCGAGC GAGCAATGGT TTTGTGATCA TTGCGCGTGC TTGTGGACAA 
TGTTTGAATT TCAATTGCGC CTGGATATTA GTGCTCATGT TGCGACATTC ACTCACATAT 
CTGCGTTCAC AGGGTCTTTC GTCGTACTTA CCACTGGATA ACCACATATA TCTCCACAAA 
CTTACGGGCA TTGTCGTGTC AATTCTCAGT CTGCTTCACA CCATCGCACA TCTTTTTAAT 
TTCTCTATAA TTGTGGTGAA CGATCCGAAA ATCAATGCTG GTCACTACAC TATTGGCGAA 
TGGCTGCTAA CCGATCGTCC AGGGCTGTTC GGTCTCATTC CGGGCTGTGC CAACCCCACC 
GGTTTAGCTC TGCTAATCAT TCTATTTATA ATGTTCATTT GCTCGCAGCC ATTCGTACGC 
CGCAAGGGTT CCTTCGAAGT CTTCTATTGG ACTCATCTGC TTTATATACC ATTTTGGATT 
CTGCTACTGT TTCATGGTCC AAATTTCTGG AAATGGTTCT TGGTGCCAGG GCTGGTGTAC 
TTTGTGGAGC GTATATTGCG ATTTGTCTGG ATGAAAAGTG AACATGGTAA AACCTACATC 
AGTTCCGGTT TACTTTTACC ATCCAAAGTC ATACATTTGG TAATCAAACG TCCATTCAAC 
TTCAATTTCC GTCCTGGTGA TTATGTCTTT GTTAATATAC CGGCTATTGC CAACTATGAA 
TGGCATCCCT TCACAATTAG TTCAGCACCG GAACAAGAGG ATTACATGTG GCTGCACATA 
CGCACTGTAG GCGAGTGGAC CAATCGTTTG TACCAGTATT TCGAGAAGGA GCAGCAGAAA 
CTGCAAAATG GCGAAATAAT ACAGCATCGA CATGCGATAC CCACCGACCT TGTGCGTATT 
ACCAACAAAA CCGAAACAAA AACATCCGCA ACGCCGCAAA TCGATTTCCT TGCTCAAAAT 
CTAGCACGTT TACATGGTAA ACCCGGTGCG AATGATTCTG TATTAGTACC TAGTAAAGAC 
AAAAGTCCAA TGCAAAATGT TCCACTCAAA CCCGCGCGAC ACACACAGCA ACCTTCGAAA 
TTGGCCATTG AAAACACCAA TGCACGTATG GAGAACGACG GTGGGCCAAC AAAACACGCA 
GATCGTATAC GCAGTATTAA GAAAACACTA CAACGTACCT TCTCACGCAA AGATGTGCCT 
GCGAAGAATG AGACGGATGG CCACTGGAAT GAAGGTTTTG TAGGCGATCA AGTGGACTCA 
GCACAAAAGC CACTGGACAA ACGTTTACAG AAGTTACACT CACGTAAACC ACAGCTCGAA 
AAGAGTCTCT CGCTACCAGA TATAGCCGTC AAGTCTAAAA AGAAGGAACG GCTCAAAGCT 
TTACGAGCGC TCGGTCGTTC GGAATCGGAA AGGTCTTTCG ACGAATCACG TGTCCGTCGT 
GCGCGACTCC AGAGCGGAGG TTTAGCCTAT TTAAGTCCAC AAAATAAGTC GCTTGCACAG 
AGTTTTCGTT ACATGCGCAA TAAGCCGACA ATAATTGCCT TTAGAACACC GAGTCTAGAA 
GAATCCGAGC CAGATTTTAC AACAACACAA AGAGGCAGCC AGGATGCATA CAGAGAACTC 
GAAGAAGGAA AAGAAAATGG AGTAGACAAG ACCACCGGCA CGGTAATTGA GGTCTCCGAA 
GCCACCGTTT CCAAGCCGTT GGAAGATCCA ACATCGCGAA CTAAAGTTGG TCGTTTTCGC 
AGACCGACCT TCTTGCGTTC CCTCTCGACA TCCATCAAAA ATCACGGTAG CAATGGCAGC 
CTACCACCAC AGGGACACCA TAACTCTGGT AGCAAGGTTA CATTAGACGC TGGCGTATTA 
GAGATCTTCA TCGATGGACC GTATGGTGCG CCGTCGAGTC ATATATTTGG TGCTCAACAT 
GCTGTGCTCA TCGGCACTGG TATCGGTGTC ACGCCGTTCG CATCTATTTT GCAATCCATA 
ATGCATCGTT ACTGGAAGGC TCGACACACC TGTCCACGTT GTCACTTTGA GTGGGCTAGT 
GACATACCGA AAACGGTGAT GAATCTGCGT AAAGTGGACT TCTTTTGGAT CAACCGCGAT 
CAGCGCTCAT TCGAGTGGTT TGTTAATCTA TTATCACAGC TGGAAATCGA ACAGGCAGAG 
TTGGGCGGAC CAATGGAGAG ATTCCTCGAT ATGCACATGT ATATAACTAG TGCACTTCAA 
CGCACCGATA TGAAGGCAGT TGGACTACAG CTGGCTTTAG ATTTACTACA TGAAAAGGGC 
AAGCGTGATC TCATTACTGG TCTGAAGACA CGCACAAATG CCGGTCGTCC GAACTGGGAT 
AAAGTCTTTA AACAGCTGCA GGCGCAGAAG AAGGGCAAGG TGACGGTATT CTACTGTGGT 
CCACCGCAAC TTGGCAAAAC ACTACGCTAT AAATGCGATC AGTATGGCTT TGCATTCCGC 
AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196275.1
CDS	473..4927
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_011197973.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011197973.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT GATGATAGAC AAATGGATTT CGCCGAACAA 
CTCGAGTCTG TGGCATATGT GATTTGTGGC GAAAATAGTA AGATCACACC GAAAAACTTT 
ACAGAAATTT GGCAAGCGCG TGGTATTCTG GATAAATTAT ACCGTCTGAT CGATGTCGAT 
GGCACAAATC TGATATCCAC AAATCAAATA ATGGAATTCA TTTCACACCT GACCAATTCA 
CGGCCACGCA CCGGTTTTGA TAAGAGCTCC CTGGTGCGTC TGGAGCAACT ATTTCGCAGC 
ACTGTAGGCA ATGAACGGGA GATTCGACGC GAAGAATTCC AGAAGATAGT CACATCGAAA 
AACCCATTCT TTACCGATCG TGTCTTTCAA ATTTTCGACA AGGATAACTC CGGTTCGATA 
TCATTGCAAG AGTTCATTGA TGCCATACAT CAATTCTCCG GCCAGTCAGC GGATGATAAA 
ATACGTTTCC TATTCAAAGT ATACGATATC GATGGTGACG GGCTGATACA GCATAAAGAA 
TTGCATGATG TGATACGCGC CTGCATGGAT GAAAATGGTA TGGAATTCTC CGAAGAACAA 
ATTGAAGATC TGACAACGGC TATGTTTGAA GATGCGGATC CATATAATCG TGGTGAAATC 
ACTTATGAAG CCTTAAAAAA TCAAATATGC AAACATGGTG GATTATTAGA GAATCTCAGT 
ATAACCATAG ATCGTTGGCT TGTGCCCATG ACGCAGGAGA ATCCGAAACC AAAGTCGAAA 
TGCCCGCGAT TGCGCTGTGG CAAGCCACAT CAACTCACTT TGGCCTACAT GAAAAACAAT 
CAAGCTTTCG TTTCGTATCT CTACATCTAC ATTGCCATAA ATGTGTGCCT CTTCATCTCA 
CGAGCCATTC AATATCGAGC GAGCAATGGT TTTGTGATCA TTGCGCGTGC TTGTGGACAA 
TGTTTGAATT TCAATTGCGC CTGGATATTA GTGCTCATGT TGCGACATTC ACTCACATAT 
CTGCGTTCAC AGGGTCTTTC GTCGTACTTA CCACTGGATA ACCACATATA TCTCCACAAA 
CTTACGGGCA TTGTCGTGTC AATTCTCAGT CTGCTTCACA CCATCGCACA TCTTTTTAAT 
TTCTCTATAA TTGTGGTGAA CGATCCGAAA ATCAATGCTG GTCACTACAC TATTGGCGAA 
TGGCTGCTAA CCGATCGTCC AGGGCTGTTC GGTCTCATTC CGGGCTGTGC CAACCCCACC 
GGTTTAGCTC TGCTAATCAT TCTATTTATA ATGTTCATTT GCTCGCAGCC ATTCGTACGC 
CGCAAGGGTT CCTTCGAAGT CTTCTATTGG ACTCATCTGC TTTATATACC ATTTTGGATT 
CTGCTACTGT TTCATGGTCC AAATTTCTGG AAATGGTTCT TGGTGCCAGG GCTGGTGTAC 
TTTGTGGAGC GTATATTGCG ATTTGTCTGG ATGAAAAGTG AACATGGTAA AACCTACATC 
AGTTCCGGTT TACTTTTACC ATCCAAAGTC ATACATTTGG TAATCAAACG TCCATTCAAC 
TTCAATTTCC GTCCTGGTGA TTATGTCTTT GTTAATATAC CGGCTATTGC CAACTATGAA 
TGGCATCCCT TCACAATTAG TTCAGCACCG GAACAAGAGG ATTACATGTG GCTGCACATA 
CGCACTGTAG GCGAGTGGAC CAATCGTTTG TACCAGTATT TCGAGAAGGA GCAGCAGAAA 
CTGCAAAATG GCGAAATAAT ACAGCATCGA CATGCGATAC CCACCGACCT TGTGCGTATT 
ACCAACAAAA CCGAAACAAA AACATCCGCA ACGCCGCAAA TCGATTTCCT TGCTCAAAAT 
CTAGCACGTT TACATGGTAA ACCCGGTGCG AATGATTCTG TATTAGTACC TAGTAAAGAC 
AAAAGTCCAA TGCAAAATGT TCCACTCAAA CCCGCGCGAC ACACACAGCA ACCTTCGAAA 
TTGGCCATTG AAAACACCAA TGCACGTATG GAGAACGACG GTGGGCCAAC AAAACACGCA 
GATCGTATAC GCAGTATTAA GAAAACACTA CAACGTACCT TCTCACGCAA AGATGTGCCT 
GCGAAGAATG AGACGGATGG CCACTGGAAT GAAGGTTTTG TAGGCGATCA AGTGGACTCA 
GCACAAAAGC CACTGGACAA ACGTTTACAG AAGTTACACT CACGTAAACC ACAGCTCGAA 
AAGAGTCTCT CGCTACCAGA TATAGCCGTC AAGTCTAAAA AGAAGGAACG GCTCAAAGCT 
TTACGAGCGC TCGGTCGTTC GGAATCGGAA AGGTCTTTCG ACGAATCACG TGTCCGTCGT 
GCGCGACTCC AGAGCGGAGG TTTAGCCTAT TTAAGTCCAC AAAATAAGTC GCTTGCACAG 
AGTTTTCGTT ACATGCGCAA TAAGCCGACA ATAATTGCCT TTAGAACACC GAGTCTAGAA 
GAATCCGAGC CAGATTTTAC AACAACACAA AGAGGCAGCC AGGATGCATA CAGAGAACTC 
GAAGAAGGAA AAGAAAATGG AGTAGACAAG ACCACCGGCA CGGTAATTGA GGTCTCCGAA 
GCCACCGTTT CCAAGCCGTT GGAAGATCCA ACATCGCGAA CTAAAGTTGG TCGTTTTCGC 
AGACCGACCT TCTTGCGTTC CCTCTCGACA TCCATCAAAA ATCACGGTAG CAATGGCAGC 
CTACCACCAC AGGGACACCA TAACTCTGGT AGCAAGGTTA CATTAGACGC TGGCGTATTA 
GAGATCTTCA TCGATGGACC GTATGGTGCG CCGTCGAGTC ATATATTTGG TGCTCAACAT 
GCTGTGCTCA TCGGCACTGG TATCGGTGTC ACGCCGTTCG CATCTATTTT GCAATCCATA 
ATGCATCGTT ACTGGAAGGC TCGACACACC TGTCCACGTT GTCACTTTGA GTGGGCTAGT 
GACATACCGA AAACGGTGAT GAATCTGCGT AAAGTGGACT TCTTTTGGAT CAACCGCGAT 
CAGCGCTCAT TCGAGTGGTT TGTTAATCTA TTATCACAGC TGGAAATCGA ACAGGCAGAG 
TTGGGCGGAC CAATGGAGAG ATTCCTCGAT ATGCACATGT ATATAACTAG TGCACTTCAA 
CGCACCGATA TGAAGGCAGT TGGACTACAG CTGGCTTTAG ATTTACTACA TGAAAAGGGC 
AAGCGTGATC TCATTACTGG TCTGAAGACA CGCACAAATG CCGGTCGTCC GAACTGGGAT 
AAAGTCTTTA AACAGCTGCA GGCGCAGAAG AAGGGCAAGG TGACGGTATT CTACTGTGGT 
CCACCGCAAC TTGGCAAAAC ACTACGCTAT AAATGCGATC AGTATGGCTT TGCATTCCGC 
AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_011197973.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196275.1 Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa193403
Clone ID Related Accession (Same CDS sequence)	XM_011197973.1 , XM_011197973.2 , XM_011197974.1
Accession Version	XM_011197973.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011197973.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT GATGATAGAC AAATGGATTT CGCCGAACAA 
CTCGAGTCTG TGGCATATGT GATTTGTGGC GAAAATAGTA AGATCACACC GAAAAACTTT 
ACAGAAATTT GGCAAGCGCG TGGTATTCTG GATAAATTAT ACCGTCTGAT CGATGTCGAT 
GGCACAAATC TGATATCCAC AAATCAAATA ATGGAATTCA TTTCACACCT GACCAATTCA 
CGGCCACGCA CCGGTTTTGA TAAGAGCTCC CTGGTGCGTC TGGAGCAACT ATTTCGCAGC 
ACTGTAGGCA ATGAACGGGA GATTCGACGC GAAGAATTCC AGAAGATAGT CACATCGAAA 
AACCCATTCT TTACCGATCG TGTCTTTCAA ATTTTCGACA AGGATAACTC CGGTTCGATA 
TCATTGCAAG AGTTCATTGA TGCCATACAT CAATTCTCCG GCCAGTCAGC GGATGATAAA 
ATACGTTTCC TATTCAAAGT ATACGATATC GATGGTGACG GGCTGATACA GCATAAAGAA 
TTGCATGATG TGATACGCGC CTGCATGGAT GAAAATGGTA TGGAATTCTC CGAAGAACAA 
ATTGAAGATC TGACAACGGC TATGTTTGAA GATGCGGATC CATATAATCG TGGTGAAATC 
ACTTATGAAG CCTTAAAAAA TCAAATATGC AAACATGGTG GATTATTAGA GAATCTCAGT 
ATAACCATAG ATCGTTGGCT TGTGCCCATG ACGCAGGAGA ATCCGAAACC AAAGTCGAAA 
TGCCCGCGAT TGCGCTGTGG CAAGCCACAT CAACTCACTT TGGCCTACAT GAAAAACAAT 
CAAGCTTTCG TTTCGTATCT CTACATCTAC ATTGCCATAA ATGTGTGCCT CTTCATCTCA 
CGAGCCATTC AATATCGAGC GAGCAATGGT TTTGTGATCA TTGCGCGTGC TTGTGGACAA 
TGTTTGAATT TCAATTGCGC CTGGATATTA GTGCTCATGT TGCGACATTC ACTCACATAT 
CTGCGTTCAC AGGGTCTTTC GTCGTACTTA CCACTGGATA ACCACATATA TCTCCACAAA 
CTTACGGGCA TTGTCGTGTC AATTCTCAGT CTGCTTCACA CCATCGCACA TCTTTTTAAT 
TTCTCTATAA TTGTGGTGAA CGATCCGAAA ATCAATGCTG GTCACTACAC TATTGGCGAA 
TGGCTGCTAA CCGATCGTCC AGGGCTGTTC GGTCTCATTC CGGGCTGTGC CAACCCCACC 
GGTTTAGCTC TGCTAATCAT TCTATTTATA ATGTTCATTT GCTCGCAGCC ATTCGTACGC 
CGCAAGGGTT CCTTCGAAGT CTTCTATTGG ACTCATCTGC TTTATATACC ATTTTGGATT 
CTGCTACTGT TTCATGGTCC AAATTTCTGG AAATGGTTCT TGGTGCCAGG GCTGGTGTAC 
TTTGTGGAGC GTATATTGCG ATTTGTCTGG ATGAAAAGTG AACATGGTAA AACCTACATC 
AGTTCCGGTT TACTTTTACC ATCCAAAGTC ATACATTTGG TAATCAAACG TCCATTCAAC 
TTCAATTTCC GTCCTGGTGA TTATGTCTTT GTTAATATAC CGGCTATTGC CAACTATGAA 
TGGCATCCCT TCACAATTAG TTCAGCACCG GAACAAGAGG ATTACATGTG GCTGCACATA 
CGCACTGTAG GCGAGTGGAC CAATCGTTTG TACCAGTATT TCGAGAAGGA GCAGCAGAAA 
CTGCAAAATG GCGAAATAAT ACAGCATCGA CATGCGATAC CCACCGACCT TGTGCGTATT 
ACCAACAAAA CCGAAACAAA AACATCCGCA ACGCCGCAAA TCGATTTCCT TGCTCAAAAT 
CTAGCACGTT TACATGGTAA ACCCGGTGCG AATGATTCTG TATTAGTACC TAGTAAAGAC 
AAAAGTCCAA TGCAAAATGT TCCACTCAAA CCCGCGCGAC ACACACAGCA ACCTTCGAAA 
TTGGCCATTG AAAACACCAA TGCACGTATG GAGAACGACG GTGGGCCAAC AAAACACGCA 
GATCGTATAC GCAGTATTAA GAAAACACTA CAACGTACCT TCTCACGCAA AGATGTGCCT 
GCGAAGAATG AGACGGATGG CCACTGGAAT GAAGGTTTTG TAGGCGATCA AGTGGACTCA 
GCACAAAAGC CACTGGACAA ACGTTTACAG AAGTTACACT CACGTAAACC ACAGCTCGAA 
AAGAGTCTCT CGCTACCAGA TATAGCCGTC AAGTCTAAAA AGAAGGAACG GCTCAAAGCT 
TTACGAGCGC TCGGTCGTTC GGAATCGGAA AGGTCTTTCG ACGAATCACG TGTCCGTCGT 
GCGCGACTCC AGAGCGGAGG TTTAGCCTAT TTAAGTCCAC AAAATAAGTC GCTTGCACAG 
AGTTTTCGTT ACATGCGCAA TAAGCCGACA ATAATTGCCT TTAGAACACC GAGTCTAGAA 
GAATCCGAGC CAGATTTTAC AACAACACAA AGAGGCAGCC AGGATGCATA CAGAGAACTC 
GAAGAAGGAA AAGAAAATGG AGTAGACAAG ACCACCGGCA CGGTAATTGA GGTCTCCGAA 
GCCACCGTTT CCAAGCCGTT GGAAGATCCA ACATCGCGAA CTAAAGTTGG TCGTTTTCGC 
AGACCGACCT TCTTGCGTTC CCTCTCGACA TCCATCAAAA ATCACGGTAG CAATGGCAGC 
CTACCACCAC AGGGACACCA TAACTCTGGT AGCAAGGTTA CATTAGACGC TGGCGTATTA 
GAGATCTTCA TCGATGGACC GTATGGTGCG CCGTCGAGTC ATATATTTGG TGCTCAACAT 
GCTGTGCTCA TCGGCACTGG TATCGGTGTC ACGCCGTTCG CATCTATTTT GCAATCCATA 
ATGCATCGTT ACTGGAAGGC TCGACACACC TGTCCACGTT GTCACTTTGA GTGGGCTAGT 
GACATACCGA AAACGGTGAT GAATCTGCGT AAAGTGGACT TCTTTTGGAT CAACCGCGAT 
CAGCGCTCAT TCGAGTGGTT TGTTAATCTA TTATCACAGC TGGAAATCGA ACAGGCAGAG 
TTGGGCGGAC CAATGGAGAG ATTCCTCGAT ATGCACATGT ATATAACTAG TGCACTTCAA 
CGCACCGATA TGAAGGCAGT TGGACTACAG CTGGCTTTAG ATTTACTACA TGAAAAGGGC 
AAGCGTGATC TCATTACTGG TCTGAAGACA CGCACAAATG CCGGTCGTCC GAACTGGGAT 
AAAGTCTTTA AACAGCTGCA GGCGCAGAAG AAGGGCAAGG TGACGGTATT CTACTGTGGT 
CCACCGCAAC TTGGCAAAAC ACTACGCTAT AAATGCGATC AGTATGGCTT TGCATTCCGC 
AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196275.1
CDS	332..4786
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_011197973.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011197973.2

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT GATGATAGAC AAATGGATTT CGCCGAACAA 
CTCGAGTCTG TGGCATATGT GATTTGTGGC GAAAATAGTA AGATCACACC GAAAAACTTT 
ACAGAAATTT GGCAAGCGCG TGGTATTCTG GATAAATTAT ACCGTCTGAT CGATGTCGAT 
GGCACAAATC TGATATCCAC AAATCAAATA ATGGAATTCA TTTCACACCT GACCAATTCA 
CGGCCACGCA CCGGTTTTGA TAAGAGCTCC CTGGTGCGTC TGGAGCAACT ATTTCGCAGC 
ACTGTAGGCA ATGAACGGGA GATTCGACGC GAAGAATTCC AGAAGATAGT CACATCGAAA 
AACCCATTCT TTACCGATCG TGTCTTTCAA ATTTTCGACA AGGATAACTC CGGTTCGATA 
TCATTGCAAG AGTTCATTGA TGCCATACAT CAATTCTCCG GCCAGTCAGC GGATGATAAA 
ATACGTTTCC TATTCAAAGT ATACGATATC GATGGTGACG GGCTGATACA GCATAAAGAA 
TTGCATGATG TGATACGCGC CTGCATGGAT GAAAATGGTA TGGAATTCTC CGAAGAACAA 
ATTGAAGATC TGACAACGGC TATGTTTGAA GATGCGGATC CATATAATCG TGGTGAAATC 
ACTTATGAAG CCTTAAAAAA TCAAATATGC AAACATGGTG GATTATTAGA GAATCTCAGT 
ATAACCATAG ATCGTTGGCT TGTGCCCATG ACGCAGGAGA ATCCGAAACC AAAGTCGAAA 
TGCCCGCGAT TGCGCTGTGG CAAGCCACAT CAACTCACTT TGGCCTACAT GAAAAACAAT 
CAAGCTTTCG TTTCGTATCT CTACATCTAC ATTGCCATAA ATGTGTGCCT CTTCATCTCA 
CGAGCCATTC AATATCGAGC GAGCAATGGT TTTGTGATCA TTGCGCGTGC TTGTGGACAA 
TGTTTGAATT TCAATTGCGC CTGGATATTA GTGCTCATGT TGCGACATTC ACTCACATAT 
CTGCGTTCAC AGGGTCTTTC GTCGTACTTA CCACTGGATA ACCACATATA TCTCCACAAA 
CTTACGGGCA TTGTCGTGTC AATTCTCAGT CTGCTTCACA CCATCGCACA TCTTTTTAAT 
TTCTCTATAA TTGTGGTGAA CGATCCGAAA ATCAATGCTG GTCACTACAC TATTGGCGAA 
TGGCTGCTAA CCGATCGTCC AGGGCTGTTC GGTCTCATTC CGGGCTGTGC CAACCCCACC 
GGTTTAGCTC TGCTAATCAT TCTATTTATA ATGTTCATTT GCTCGCAGCC ATTCGTACGC 
CGCAAGGGTT CCTTCGAAGT CTTCTATTGG ACTCATCTGC TTTATATACC ATTTTGGATT 
CTGCTACTGT TTCATGGTCC AAATTTCTGG AAATGGTTCT TGGTGCCAGG GCTGGTGTAC 
TTTGTGGAGC GTATATTGCG ATTTGTCTGG ATGAAAAGTG AACATGGTAA AACCTACATC 
AGTTCCGGTT TACTTTTACC ATCCAAAGTC ATACATTTGG TAATCAAACG TCCATTCAAC 
TTCAATTTCC GTCCTGGTGA TTATGTCTTT GTTAATATAC CGGCTATTGC CAACTATGAA 
TGGCATCCCT TCACAATTAG TTCAGCACCG GAACAAGAGG ATTACATGTG GCTGCACATA 
CGCACTGTAG GCGAGTGGAC CAATCGTTTG TACCAGTATT TCGAGAAGGA GCAGCAGAAA 
CTGCAAAATG GCGAAATAAT ACAGCATCGA CATGCGATAC CCACCGACCT TGTGCGTATT 
ACCAACAAAA CCGAAACAAA AACATCCGCA ACGCCGCAAA TCGATTTCCT TGCTCAAAAT 
CTAGCACGTT TACATGGTAA ACCCGGTGCG AATGATTCTG TATTAGTACC TAGTAAAGAC 
AAAAGTCCAA TGCAAAATGT TCCACTCAAA CCCGCGCGAC ACACACAGCA ACCTTCGAAA 
TTGGCCATTG AAAACACCAA TGCACGTATG GAGAACGACG GTGGGCCAAC AAAACACGCA 
GATCGTATAC GCAGTATTAA GAAAACACTA CAACGTACCT TCTCACGCAA AGATGTGCCT 
GCGAAGAATG AGACGGATGG CCACTGGAAT GAAGGTTTTG TAGGCGATCA AGTGGACTCA 
GCACAAAAGC CACTGGACAA ACGTTTACAG AAGTTACACT CACGTAAACC ACAGCTCGAA 
AAGAGTCTCT CGCTACCAGA TATAGCCGTC AAGTCTAAAA AGAAGGAACG GCTCAAAGCT 
TTACGAGCGC TCGGTCGTTC GGAATCGGAA AGGTCTTTCG ACGAATCACG TGTCCGTCGT 
GCGCGACTCC AGAGCGGAGG TTTAGCCTAT TTAAGTCCAC AAAATAAGTC GCTTGCACAG 
AGTTTTCGTT ACATGCGCAA TAAGCCGACA ATAATTGCCT TTAGAACACC GAGTCTAGAA 
GAATCCGAGC CAGATTTTAC AACAACACAA AGAGGCAGCC AGGATGCATA CAGAGAACTC 
GAAGAAGGAA AAGAAAATGG AGTAGACAAG ACCACCGGCA CGGTAATTGA GGTCTCCGAA 
GCCACCGTTT CCAAGCCGTT GGAAGATCCA ACATCGCGAA CTAAAGTTGG TCGTTTTCGC 
AGACCGACCT TCTTGCGTTC CCTCTCGACA TCCATCAAAA ATCACGGTAG CAATGGCAGC 
CTACCACCAC AGGGACACCA TAACTCTGGT AGCAAGGTTA CATTAGACGC TGGCGTATTA 
GAGATCTTCA TCGATGGACC GTATGGTGCG CCGTCGAGTC ATATATTTGG TGCTCAACAT 
GCTGTGCTCA TCGGCACTGG TATCGGTGTC ACGCCGTTCG CATCTATTTT GCAATCCATA 
ATGCATCGTT ACTGGAAGGC TCGACACACC TGTCCACGTT GTCACTTTGA GTGGGCTAGT 
GACATACCGA AAACGGTGAT GAATCTGCGT AAAGTGGACT TCTTTTGGAT CAACCGCGAT 
CAGCGCTCAT TCGAGTGGTT TGTTAATCTA TTATCACAGC TGGAAATCGA ACAGGCAGAG 
TTGGGCGGAC CAATGGAGAG ATTCCTCGAT ATGCACATGT ATATAACTAG TGCACTTCAA 
CGCACCGATA TGAAGGCAGT TGGACTACAG CTGGCTTTAG ATTTACTACA TGAAAAGGGC 
AAGCGTGATC TCATTACTGG TCTGAAGACA CGCACAAATG CCGGTCGTCC GAACTGGGAT 
AAAGTCTTTA AACAGCTGCA GGCGCAGAAG AAGGGCAAGG TGACGGTATT CTACTGTGGT 
CCACCGCAAC TTGGCAAAAC ACTACGCTAT AAATGCGATC AGTATGGCTT TGCATTCCGC 
AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_011197974.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196276.1 Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X2, mRNA.

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CloneID	OBa193403
Clone ID Related Accession (Same CDS sequence)	XM_011197973.1 , XM_011197973.2 , XM_011197974.1
Accession Version	XM_011197974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT GATGATAGAC AAATGGATTT CGCCGAACAA 
CTCGAGTCTG TGGCATATGT GATTTGTGGC GAAAATAGTA AGATCACACC GAAAAACTTT 
ACAGAAATTT GGCAAGCGCG TGGTATTCTG GATAAATTAT ACCGTCTGAT CGATGTCGAT 
GGCACAAATC TGATATCCAC AAATCAAATA ATGGAATTCA TTTCACACCT GACCAATTCA 
CGGCCACGCA CCGGTTTTGA TAAGAGCTCC CTGGTGCGTC TGGAGCAACT ATTTCGCAGC 
ACTGTAGGCA ATGAACGGGA GATTCGACGC GAAGAATTCC AGAAGATAGT CACATCGAAA 
AACCCATTCT TTACCGATCG TGTCTTTCAA ATTTTCGACA AGGATAACTC CGGTTCGATA 
TCATTGCAAG AGTTCATTGA TGCCATACAT CAATTCTCCG GCCAGTCAGC GGATGATAAA 
ATACGTTTCC TATTCAAAGT ATACGATATC GATGGTGACG GGCTGATACA GCATAAAGAA 
TTGCATGATG TGATACGCGC CTGCATGGAT GAAAATGGTA TGGAATTCTC CGAAGAACAA 
ATTGAAGATC TGACAACGGC TATGTTTGAA GATGCGGATC CATATAATCG TGGTGAAATC 
ACTTATGAAG CCTTAAAAAA TCAAATATGC AAACATGGTG GATTATTAGA GAATCTCAGT 
ATAACCATAG ATCGTTGGCT TGTGCCCATG ACGCAGGAGA ATCCGAAACC AAAGTCGAAA 
TGCCCGCGAT TGCGCTGTGG CAAGCCACAT CAACTCACTT TGGCCTACAT GAAAAACAAT 
CAAGCTTTCG TTTCGTATCT CTACATCTAC ATTGCCATAA ATGTGTGCCT CTTCATCTCA 
CGAGCCATTC AATATCGAGC GAGCAATGGT TTTGTGATCA TTGCGCGTGC TTGTGGACAA 
TGTTTGAATT TCAATTGCGC CTGGATATTA GTGCTCATGT TGCGACATTC ACTCACATAT 
CTGCGTTCAC AGGGTCTTTC GTCGTACTTA CCACTGGATA ACCACATATA TCTCCACAAA 
CTTACGGGCA TTGTCGTGTC AATTCTCAGT CTGCTTCACA CCATCGCACA TCTTTTTAAT 
TTCTCTATAA TTGTGGTGAA CGATCCGAAA ATCAATGCTG GTCACTACAC TATTGGCGAA 
TGGCTGCTAA CCGATCGTCC AGGGCTGTTC GGTCTCATTC CGGGCTGTGC CAACCCCACC 
GGTTTAGCTC TGCTAATCAT TCTATTTATA ATGTTCATTT GCTCGCAGCC ATTCGTACGC 
CGCAAGGGTT CCTTCGAAGT CTTCTATTGG ACTCATCTGC TTTATATACC ATTTTGGATT 
CTGCTACTGT TTCATGGTCC AAATTTCTGG AAATGGTTCT TGGTGCCAGG GCTGGTGTAC 
TTTGTGGAGC GTATATTGCG ATTTGTCTGG ATGAAAAGTG AACATGGTAA AACCTACATC 
AGTTCCGGTT TACTTTTACC ATCCAAAGTC ATACATTTGG TAATCAAACG TCCATTCAAC 
TTCAATTTCC GTCCTGGTGA TTATGTCTTT GTTAATATAC CGGCTATTGC CAACTATGAA 
TGGCATCCCT TCACAATTAG TTCAGCACCG GAACAAGAGG ATTACATGTG GCTGCACATA 
CGCACTGTAG GCGAGTGGAC CAATCGTTTG TACCAGTATT TCGAGAAGGA GCAGCAGAAA 
CTGCAAAATG GCGAAATAAT ACAGCATCGA CATGCGATAC CCACCGACCT TGTGCGTATT 
ACCAACAAAA CCGAAACAAA AACATCCGCA ACGCCGCAAA TCGATTTCCT TGCTCAAAAT 
CTAGCACGTT TACATGGTAA ACCCGGTGCG AATGATTCTG TATTAGTACC TAGTAAAGAC 
AAAAGTCCAA TGCAAAATGT TCCACTCAAA CCCGCGCGAC ACACACAGCA ACCTTCGAAA 
TTGGCCATTG AAAACACCAA TGCACGTATG GAGAACGACG GTGGGCCAAC AAAACACGCA 
GATCGTATAC GCAGTATTAA GAAAACACTA CAACGTACCT TCTCACGCAA AGATGTGCCT 
GCGAAGAATG AGACGGATGG CCACTGGAAT GAAGGTTTTG TAGGCGATCA AGTGGACTCA 
GCACAAAAGC CACTGGACAA ACGTTTACAG AAGTTACACT CACGTAAACC ACAGCTCGAA 
AAGAGTCTCT CGCTACCAGA TATAGCCGTC AAGTCTAAAA AGAAGGAACG GCTCAAAGCT 
TTACGAGCGC TCGGTCGTTC GGAATCGGAA AGGTCTTTCG ACGAATCACG TGTCCGTCGT 
GCGCGACTCC AGAGCGGAGG TTTAGCCTAT TTAAGTCCAC AAAATAAGTC GCTTGCACAG 
AGTTTTCGTT ACATGCGCAA TAAGCCGACA ATAATTGCCT TTAGAACACC GAGTCTAGAA 
GAATCCGAGC CAGATTTTAC AACAACACAA AGAGGCAGCC AGGATGCATA CAGAGAACTC 
GAAGAAGGAA AAGAAAATGG AGTAGACAAG ACCACCGGCA CGGTAATTGA GGTCTCCGAA 
GCCACCGTTT CCAAGCCGTT GGAAGATCCA ACATCGCGAA CTAAAGTTGG TCGTTTTCGC 
AGACCGACCT TCTTGCGTTC CCTCTCGACA TCCATCAAAA ATCACGGTAG CAATGGCAGC 
CTACCACCAC AGGGACACCA TAACTCTGGT AGCAAGGTTA CATTAGACGC TGGCGTATTA 
GAGATCTTCA TCGATGGACC GTATGGTGCG CCGTCGAGTC ATATATTTGG TGCTCAACAT 
GCTGTGCTCA TCGGCACTGG TATCGGTGTC ACGCCGTTCG CATCTATTTT GCAATCCATA 
ATGCATCGTT ACTGGAAGGC TCGACACACC TGTCCACGTT GTCACTTTGA GTGGGCTAGT 
GACATACCGA AAACGGTGAT GAATCTGCGT AAAGTGGACT TCTTTTGGAT CAACCGCGAT 
CAGCGCTCAT TCGAGTGGTT TGTTAATCTA TTATCACAGC TGGAAATCGA ACAGGCAGAG 
TTGGGCGGAC CAATGGAGAG ATTCCTCGAT ATGCACATGT ATATAACTAG TGCACTTCAA 
CGCACCGATA TGAAGGCAGT TGGACTACAG CTGGCTTTAG ATTTACTACA TGAAAAGGGC 
AAGCGTGATC TCATTACTGG TCTGAAGACA CGCACAAATG CCGGTCGTCC GAACTGGGAT 
AAAGTCTTTA AACAGCTGCA GGCGCAGAAG AAGGGCAAGG TGACGGTATT CTACTGTGGT 
CCACCGCAAC TTGGCAAAAC ACTACGCTAT AAATGCGATC AGTATGGCTT TGCATTCCGC 
AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196276.1
CDS	430..4884
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_011197974.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011197974.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT GATGATAGAC AAATGGATTT CGCCGAACAA 
CTCGAGTCTG TGGCATATGT GATTTGTGGC GAAAATAGTA AGATCACACC GAAAAACTTT 
ACAGAAATTT GGCAAGCGCG TGGTATTCTG GATAAATTAT ACCGTCTGAT CGATGTCGAT 
GGCACAAATC TGATATCCAC AAATCAAATA ATGGAATTCA TTTCACACCT GACCAATTCA 
CGGCCACGCA CCGGTTTTGA TAAGAGCTCC CTGGTGCGTC TGGAGCAACT ATTTCGCAGC 
ACTGTAGGCA ATGAACGGGA GATTCGACGC GAAGAATTCC AGAAGATAGT CACATCGAAA 
AACCCATTCT TTACCGATCG TGTCTTTCAA ATTTTCGACA AGGATAACTC CGGTTCGATA 
TCATTGCAAG AGTTCATTGA TGCCATACAT CAATTCTCCG GCCAGTCAGC GGATGATAAA 
ATACGTTTCC TATTCAAAGT ATACGATATC GATGGTGACG GGCTGATACA GCATAAAGAA 
TTGCATGATG TGATACGCGC CTGCATGGAT GAAAATGGTA TGGAATTCTC CGAAGAACAA 
ATTGAAGATC TGACAACGGC TATGTTTGAA GATGCGGATC CATATAATCG TGGTGAAATC 
ACTTATGAAG CCTTAAAAAA TCAAATATGC AAACATGGTG GATTATTAGA GAATCTCAGT 
ATAACCATAG ATCGTTGGCT TGTGCCCATG ACGCAGGAGA ATCCGAAACC AAAGTCGAAA 
TGCCCGCGAT TGCGCTGTGG CAAGCCACAT CAACTCACTT TGGCCTACAT GAAAAACAAT 
CAAGCTTTCG TTTCGTATCT CTACATCTAC ATTGCCATAA ATGTGTGCCT CTTCATCTCA 
CGAGCCATTC AATATCGAGC GAGCAATGGT TTTGTGATCA TTGCGCGTGC TTGTGGACAA 
TGTTTGAATT TCAATTGCGC CTGGATATTA GTGCTCATGT TGCGACATTC ACTCACATAT 
CTGCGTTCAC AGGGTCTTTC GTCGTACTTA CCACTGGATA ACCACATATA TCTCCACAAA 
CTTACGGGCA TTGTCGTGTC AATTCTCAGT CTGCTTCACA CCATCGCACA TCTTTTTAAT 
TTCTCTATAA TTGTGGTGAA CGATCCGAAA ATCAATGCTG GTCACTACAC TATTGGCGAA 
TGGCTGCTAA CCGATCGTCC AGGGCTGTTC GGTCTCATTC CGGGCTGTGC CAACCCCACC 
GGTTTAGCTC TGCTAATCAT TCTATTTATA ATGTTCATTT GCTCGCAGCC ATTCGTACGC 
CGCAAGGGTT CCTTCGAAGT CTTCTATTGG ACTCATCTGC TTTATATACC ATTTTGGATT 
CTGCTACTGT TTCATGGTCC AAATTTCTGG AAATGGTTCT TGGTGCCAGG GCTGGTGTAC 
TTTGTGGAGC GTATATTGCG ATTTGTCTGG ATGAAAAGTG AACATGGTAA AACCTACATC 
AGTTCCGGTT TACTTTTACC ATCCAAAGTC ATACATTTGG TAATCAAACG TCCATTCAAC 
TTCAATTTCC GTCCTGGTGA TTATGTCTTT GTTAATATAC CGGCTATTGC CAACTATGAA 
TGGCATCCCT TCACAATTAG TTCAGCACCG GAACAAGAGG ATTACATGTG GCTGCACATA 
CGCACTGTAG GCGAGTGGAC CAATCGTTTG TACCAGTATT TCGAGAAGGA GCAGCAGAAA 
CTGCAAAATG GCGAAATAAT ACAGCATCGA CATGCGATAC CCACCGACCT TGTGCGTATT 
ACCAACAAAA CCGAAACAAA AACATCCGCA ACGCCGCAAA TCGATTTCCT TGCTCAAAAT 
CTAGCACGTT TACATGGTAA ACCCGGTGCG AATGATTCTG TATTAGTACC TAGTAAAGAC 
AAAAGTCCAA TGCAAAATGT TCCACTCAAA CCCGCGCGAC ACACACAGCA ACCTTCGAAA 
TTGGCCATTG AAAACACCAA TGCACGTATG GAGAACGACG GTGGGCCAAC AAAACACGCA 
GATCGTATAC GCAGTATTAA GAAAACACTA CAACGTACCT TCTCACGCAA AGATGTGCCT 
GCGAAGAATG AGACGGATGG CCACTGGAAT GAAGGTTTTG TAGGCGATCA AGTGGACTCA 
GCACAAAAGC CACTGGACAA ACGTTTACAG AAGTTACACT CACGTAAACC ACAGCTCGAA 
AAGAGTCTCT CGCTACCAGA TATAGCCGTC AAGTCTAAAA AGAAGGAACG GCTCAAAGCT 
TTACGAGCGC TCGGTCGTTC GGAATCGGAA AGGTCTTTCG ACGAATCACG TGTCCGTCGT 
GCGCGACTCC AGAGCGGAGG TTTAGCCTAT TTAAGTCCAC AAAATAAGTC GCTTGCACAG 
AGTTTTCGTT ACATGCGCAA TAAGCCGACA ATAATTGCCT TTAGAACACC GAGTCTAGAA 
GAATCCGAGC CAGATTTTAC AACAACACAA AGAGGCAGCC AGGATGCATA CAGAGAACTC 
GAAGAAGGAA AAGAAAATGG AGTAGACAAG ACCACCGGCA CGGTAATTGA GGTCTCCGAA 
GCCACCGTTT CCAAGCCGTT GGAAGATCCA ACATCGCGAA CTAAAGTTGG TCGTTTTCGC 
AGACCGACCT TCTTGCGTTC CCTCTCGACA TCCATCAAAA ATCACGGTAG CAATGGCAGC 
CTACCACCAC AGGGACACCA TAACTCTGGT AGCAAGGTTA CATTAGACGC TGGCGTATTA 
GAGATCTTCA TCGATGGACC GTATGGTGCG CCGTCGAGTC ATATATTTGG TGCTCAACAT 
GCTGTGCTCA TCGGCACTGG TATCGGTGTC ACGCCGTTCG CATCTATTTT GCAATCCATA 
ATGCATCGTT ACTGGAAGGC TCGACACACC TGTCCACGTT GTCACTTTGA GTGGGCTAGT 
GACATACCGA AAACGGTGAT GAATCTGCGT AAAGTGGACT TCTTTTGGAT CAACCGCGAT 
CAGCGCTCAT TCGAGTGGTT TGTTAATCTA TTATCACAGC TGGAAATCGA ACAGGCAGAG 
TTGGGCGGAC CAATGGAGAG ATTCCTCGAT ATGCACATGT ATATAACTAG TGCACTTCAA 
CGCACCGATA TGAAGGCAGT TGGACTACAG CTGGCTTTAG ATTTACTACA TGAAAAGGGC 
AAGCGTGATC TCATTACTGG TCTGAAGACA CGCACAAATG CCGGTCGTCC GAACTGGGAT 
AAAGTCTTTA AACAGCTGCA GGCGCAGAAG AAGGGCAAGG TGACGGTATT CTACTGTGGT 
CCACCGCAAC TTGGCAAAAC ACTACGCTAT AAATGCGATC AGTATGGCTT TGCATTCCGC 
AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_011197975.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011196277.1 Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X3, mRNA.

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CloneID	OBa193404
Clone ID Related Accession (Same CDS sequence)	XM_011197975.1
Accession Version	XM_011197975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT GATGATAGAC AAATGGATTT CGCCGAACAA 
CTCGAGTCTG TGGCATATGT GATTTGTGGC GAAAATAGTA AGATCACACC GAAAAACTTT 
ACAGAAATTT GGCAAGCGCG TGGTATTCTG GATAAATTAT ACCGTCTGAT CGATGTCGAT 
GGCACAAATC TGATATCCAC AAATCAAATA ATGGAATTCA TTTCACACCT GACCAATTCA 
CGGCCACGCA CCGGTTTTGA TAAGAGCTCC CTGGTGCGTC TGGAGCAACT ATTTCGCAGC 
ACTGTAGGCA ATGAACGGGA GATTCGACGC GAAGAATTCC AGAAGATAGT CACATCGAAA 
AACCCATTCT TTACCGATCG TGTCTTTCAA ATTTTCGACA AGGATAACTC CGGTTCGATA 
TCATTGCAAG AGTTCATTGA TGCCATACAT CAATTCTCCG GCCAGTCAGC GGATGATAAA 
ATACGTTTCC TATTCAAAGT ATACGATATC GATGGTGACG GGCTGATACA GCATAAAGAA 
TTGCATGATG TGATACGCGC CTGCATGGAT GAAAATGGTA TGGAATTCTC CGAAGAACAA 
ATTGAAGATC TGACAACGGC TATGTTTGAA GATGCGGATC CATATAATCG TGGTGAAATC 
ACTTATGAAG CCTTAAAAAA TCAAATATGC AAACATGGTG GATTATTAGA GAATCTCAGT 
ATAACCATAG ATCGTTGGCT TGTGCCCATG ACGCAGGAGA ATCCGAAACC AAAGTCGAAA 
TGCCCGCGAT TGCGCTGTGG CAAGCCACAT CAACTCACTT TGGCCTACAT GAAAAACAAT 
CAAGCTTTCG TTTCGTATCT CTACATCTAC ATTGCCATAA ATGTGTGCCT CTTCATCTCA 
CGAGCCATTC AATATCGAGC GAGCAATGGT TTTGTGATCA TTGCGCGTGC TTGTGGACAA 
TGTTTGAATT TCAATTGCGC CTGGATATTA GTGCTCATGT TGCGACATTC ACTCACATAT 
CTGCGTTCAC AGGGTCTTTC GTCGTACTTA CCACTGGATA ACCACATATA TCTCCACAAA 
CTTACGGGCA TTGTCGTGTC AATTCTCAGT CTGCTTCACA CCATCGCACA TCTTTTTAAT 
TTCTCTATAA TTGTGGTGAA CGATCCGAAA ATCAATGCTG GTCACTACAC TATTGGCGAA 
TGGCTGCTAA CCGATCGTCC AGGGCTGTTC GGTCTCATTC CGGGCTGTGC CAACCCCACC 
GGTTTAGCTC TGCTAATCAT TCTATTTATA ATGTTCATTT GCTCGCAGCC ATTCGTACGC 
CGCAAGGGTT CCTTCGAAGT CTTCTATTGG ACTCATCTGC TTTATATACC ATTTTGGATT 
CTGCTACTGT TTCATGGTCC AAATTTCTGG AAATGGTTCT TGGTGCCAGG GCTGGTGTAC 
TTTGTGGAGC GTATATTGCG ATTTGTCTGG ATGAAAAGTG AACATGGTAA AACCTACATC 
AGTTCCGGTT TACTTTTACC ATCCAAAGTC ATACATTTGG TAATCAAACG TCCATTCAAC 
TTCAATTTCC GTCCTGGTGA TTATGTCTTT GTTAATATAC CGGCTATTGC CAACTATGAA 
TGGCATCCCT TCACAATTAG TTCAGCACCG GAACAAGAGG ATTACATGTG GCTGCACATA 
CGCACTGTAG GCGAGTGGAC CAATCGTTTG TACCAGTATT TCGAGAAGGA GCAGCAGAAA 
CTGCAAAATG GCGAAATAAT ACAGCATCGA CATGCGATAC CCACCGACCT TGTGCGTATT 
ACCAACAAAA CCGAAACAAA AACATCCGCA ACGCCGCAAA TCGATTTCCT TGCTCAAAAT 
CTAGCACGTT TACATGGTAA ACCCGGTGCG AATGATTCTG TATTAGTACC TAGTAAAGAC 
AAAAGTCCAA TGCAAAATGT TCCACTCAAA CCCGCGCGAC ACACACAGCA ACCTTCGAAA 
TTGGCCATTG AAAACACCAA TGCACGTATG GAGAACGACG GTGGGCCAAC AAAACACGCA 
GATCGTATAC GCAGTATTAA GAAAACACTA CAACGTACCT TCTCACGCAA AGATGTGCCT 
GCGAAGAATG AGACGGATGG CCACTGGAAT GAAGGTTTTG TAGGCGATCA AGTGGACTCA 
GCACAAAAGC CACTGGACAA ACGTTTACAG AAGTTACACT CACGTAAACC ACAGCTCGAA 
AAGAGTCTCT CGCTACCAGA TATAGCCGTC AAGTCTAAAA AGAAGGAACG GCTCAAAGCT 
TTACGAGCGC TCGGTCGTTC GGAATCGGAA AGGTCTTTCG ACGAATCACG TGTCCGTCGT 
GCGCGACTCC AGAGCGGAGG TTTAGCCTAT TTAAGTCCAC AAAATAAGTC GCTTGCACAG 
AGTTTTCGTT ACATGCGCAA TAAGCCGACA ATAATTGCCT TTAGAACACC GAGTCTAGAA 
GAATCCGAGC CAGATTTTAC AACAACACAA AGAGGCAGCC AGGATGCATA CAGAGAACTC 
GAAGAAGGAA AAGAAAATGG AGTAGACAAG ACCACCGGCA CGGTAATTGA GGTCTCCGAA 
GCCACCGTTT CCAAGCCGTT GGAAATCTTC ATCGATGGAC CGTATGGTGC GCCGTCGAGT 
CATATATTTG GTGCTCAACA TGCTGTGCTC ATCGGCACTG GTATCGGTGT CACGCCGTTC 
GCATCTATTT TGCAATCCAT AATGCATCGT TACTGGAAGG CTCGACACAC CTGTCCACGT 
TGTCACTTTG AGTGGGCTAG TGACATACCG AAAACGGTGA TGAATCTGCG TAAAGTGGAC 
TTCTTTTGGA TCAACCGCGA TCAGCGCTCA TTCGAGTGGT TTGTTAATCT ATTATCACAG 
CTGGAAATCG AACAGGCAGA GTTGGGCGGA CCAATGGAGA GATTCCTCGA TATGCACATG 
TATATAACTA GTGCACTTCA ACGCACCGAT ATGAAGGCAG TTGGACTACA GCTGGCTTTA 
GATTTACTAC ATGAAAAGGG CAAGCGTGAT CTCATTACTG GTCTGAAGAC ACGCACAAAT 
GCCGGTCGTC CGAACTGGGA TAAAGTCTTT AAACAGCTGC AGGCGCAGAA GAAGGGCAAG 
GTGACGGTAT TCTACTGTGG TCCACCGCAA CTTGGCAAAA CACTACGCTA TAAATGCGAT 
CAGTATGGCT TTGCATTCCG CAAAGAATGT TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011196277.1
CDS	474..4769
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_011197975.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011197975.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT GATGATAGAC AAATGGATTT CGCCGAACAA 
CTCGAGTCTG TGGCATATGT GATTTGTGGC GAAAATAGTA AGATCACACC GAAAAACTTT 
ACAGAAATTT GGCAAGCGCG TGGTATTCTG GATAAATTAT ACCGTCTGAT CGATGTCGAT 
GGCACAAATC TGATATCCAC AAATCAAATA ATGGAATTCA TTTCACACCT GACCAATTCA 
CGGCCACGCA CCGGTTTTGA TAAGAGCTCC CTGGTGCGTC TGGAGCAACT ATTTCGCAGC 
ACTGTAGGCA ATGAACGGGA GATTCGACGC GAAGAATTCC AGAAGATAGT CACATCGAAA 
AACCCATTCT TTACCGATCG TGTCTTTCAA ATTTTCGACA AGGATAACTC CGGTTCGATA 
TCATTGCAAG AGTTCATTGA TGCCATACAT CAATTCTCCG GCCAGTCAGC GGATGATAAA 
ATACGTTTCC TATTCAAAGT ATACGATATC GATGGTGACG GGCTGATACA GCATAAAGAA 
TTGCATGATG TGATACGCGC CTGCATGGAT GAAAATGGTA TGGAATTCTC CGAAGAACAA 
ATTGAAGATC TGACAACGGC TATGTTTGAA GATGCGGATC CATATAATCG TGGTGAAATC 
ACTTATGAAG CCTTAAAAAA TCAAATATGC AAACATGGTG GATTATTAGA GAATCTCAGT 
ATAACCATAG ATCGTTGGCT TGTGCCCATG ACGCAGGAGA ATCCGAAACC AAAGTCGAAA 
TGCCCGCGAT TGCGCTGTGG CAAGCCACAT CAACTCACTT TGGCCTACAT GAAAAACAAT 
CAAGCTTTCG TTTCGTATCT CTACATCTAC ATTGCCATAA ATGTGTGCCT CTTCATCTCA 
CGAGCCATTC AATATCGAGC GAGCAATGGT TTTGTGATCA TTGCGCGTGC TTGTGGACAA 
TGTTTGAATT TCAATTGCGC CTGGATATTA GTGCTCATGT TGCGACATTC ACTCACATAT 
CTGCGTTCAC AGGGTCTTTC GTCGTACTTA CCACTGGATA ACCACATATA TCTCCACAAA 
CTTACGGGCA TTGTCGTGTC AATTCTCAGT CTGCTTCACA CCATCGCACA TCTTTTTAAT 
TTCTCTATAA TTGTGGTGAA CGATCCGAAA ATCAATGCTG GTCACTACAC TATTGGCGAA 
TGGCTGCTAA CCGATCGTCC AGGGCTGTTC GGTCTCATTC CGGGCTGTGC CAACCCCACC 
GGTTTAGCTC TGCTAATCAT TCTATTTATA ATGTTCATTT GCTCGCAGCC ATTCGTACGC 
CGCAAGGGTT CCTTCGAAGT CTTCTATTGG ACTCATCTGC TTTATATACC ATTTTGGATT 
CTGCTACTGT TTCATGGTCC AAATTTCTGG AAATGGTTCT TGGTGCCAGG GCTGGTGTAC 
TTTGTGGAGC GTATATTGCG ATTTGTCTGG ATGAAAAGTG AACATGGTAA AACCTACATC 
AGTTCCGGTT TACTTTTACC ATCCAAAGTC ATACATTTGG TAATCAAACG TCCATTCAAC 
TTCAATTTCC GTCCTGGTGA TTATGTCTTT GTTAATATAC CGGCTATTGC CAACTATGAA 
TGGCATCCCT TCACAATTAG TTCAGCACCG GAACAAGAGG ATTACATGTG GCTGCACATA 
CGCACTGTAG GCGAGTGGAC CAATCGTTTG TACCAGTATT TCGAGAAGGA GCAGCAGAAA 
CTGCAAAATG GCGAAATAAT ACAGCATCGA CATGCGATAC CCACCGACCT TGTGCGTATT 
ACCAACAAAA CCGAAACAAA AACATCCGCA ACGCCGCAAA TCGATTTCCT TGCTCAAAAT 
CTAGCACGTT TACATGGTAA ACCCGGTGCG AATGATTCTG TATTAGTACC TAGTAAAGAC 
AAAAGTCCAA TGCAAAATGT TCCACTCAAA CCCGCGCGAC ACACACAGCA ACCTTCGAAA 
TTGGCCATTG AAAACACCAA TGCACGTATG GAGAACGACG GTGGGCCAAC AAAACACGCA 
GATCGTATAC GCAGTATTAA GAAAACACTA CAACGTACCT TCTCACGCAA AGATGTGCCT 
GCGAAGAATG AGACGGATGG CCACTGGAAT GAAGGTTTTG TAGGCGATCA AGTGGACTCA 
GCACAAAAGC CACTGGACAA ACGTTTACAG AAGTTACACT CACGTAAACC ACAGCTCGAA 
AAGAGTCTCT CGCTACCAGA TATAGCCGTC AAGTCTAAAA AGAAGGAACG GCTCAAAGCT 
TTACGAGCGC TCGGTCGTTC GGAATCGGAA AGGTCTTTCG ACGAATCACG TGTCCGTCGT 
GCGCGACTCC AGAGCGGAGG TTTAGCCTAT TTAAGTCCAC AAAATAAGTC GCTTGCACAG 
AGTTTTCGTT ACATGCGCAA TAAGCCGACA ATAATTGCCT TTAGAACACC GAGTCTAGAA 
GAATCCGAGC CAGATTTTAC AACAACACAA AGAGGCAGCC AGGATGCATA CAGAGAACTC 
GAAGAAGGAA AAGAAAATGG AGTAGACAAG ACCACCGGCA CGGTAATTGA GGTCTCCGAA 
GCCACCGTTT CCAAGCCGTT GGAAATCTTC ATCGATGGAC CGTATGGTGC GCCGTCGAGT 
CATATATTTG GTGCTCAACA TGCTGTGCTC ATCGGCACTG GTATCGGTGT CACGCCGTTC 
GCATCTATTT TGCAATCCAT AATGCATCGT TACTGGAAGG CTCGACACAC CTGTCCACGT 
TGTCACTTTG AGTGGGCTAG TGACATACCG AAAACGGTGA TGAATCTGCG TAAAGTGGAC 
TTCTTTTGGA TCAACCGCGA TCAGCGCTCA TTCGAGTGGT TTGTTAATCT ATTATCACAG 
CTGGAAATCG AACAGGCAGA GTTGGGCGGA CCAATGGAGA GATTCCTCGA TATGCACATG 
TATATAACTA GTGCACTTCA ACGCACCGAT ATGAAGGCAG TTGGACTACA GCTGGCTTTA 
GATTTACTAC ATGAAAAGGG CAAGCGTGAT CTCATTACTG GTCTGAAGAC ACGCACAAAT 
GCCGGTCGTC CGAACTGGGA TAAAGTCTTT AAACAGCTGC AGGCGCAGAA GAAGGGCAAG 
GTGACGGTAT TCTACTGTGG TCCACCGCAA CTTGGCAAAA CACTACGCTA TAAATGCGAT 
CAGTATGGCT TTGCATTCCG CAAAGAATGT TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_029046294.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028902127.1 Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X1, mRNA.

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CloneID	OBa254280
Clone ID Related Accession (Same CDS sequence)	XM_029046294.1 , XM_029046296.1 , XM_029046297.1 , XM_029046298.1 , XM_029046295.1 , XM_029046299.1
Accession Version	XM_029046294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902127.1
CDS	520..5004
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTPLDSTYADSCDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_029046294.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046294.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_029046296.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028902129.1 Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa254280
Clone ID Related Accession (Same CDS sequence)	XM_029046294.1 , XM_029046296.1 , XM_029046297.1 , XM_029046298.1 , XM_029046295.1 , XM_029046299.1
Accession Version	XM_029046296.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902129.1
CDS	326..4810
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTPLDSTYADSCDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_029046296.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046296.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_029046297.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028902130.1 Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X4, mRNA.

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CloneID	OBa254280
Clone ID Related Accession (Same CDS sequence)	XM_029046294.1 , XM_029046296.1 , XM_029046297.1 , XM_029046298.1 , XM_029046295.1 , XM_029046299.1
Accession Version	XM_029046297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902130.1
CDS	419..4903
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTPLDSTYADSCDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_029046297.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046297.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_029046298.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028902131.1 Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X5, mRNA.

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CloneID	OBa254280
Clone ID Related Accession (Same CDS sequence)	XM_029046294.1 , XM_029046296.1 , XM_029046297.1 , XM_029046298.1 , XM_029046295.1 , XM_029046299.1
Accession Version	XM_029046298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902131.1
CDS	235..4719
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTPLDSTYADSCDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_029046298.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046298.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_029046295.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028902128.1 Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X2, mRNA.

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CloneID	OBa254280
Clone ID Related Accession (Same CDS sequence)	XM_029046294.1 , XM_029046296.1 , XM_029046297.1 , XM_029046298.1 , XM_029046295.1 , XM_029046299.1
Accession Version	XM_029046295.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902128.1
CDS	271..4755
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTPLDSTYADSCDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_029046295.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046295.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105221203 ( XM_029046299.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_028902132.1 Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X6, mRNA.

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CloneID	OBa254280
Clone ID Related Accession (Same CDS sequence)	XM_029046294.1 , XM_029046296.1 , XM_029046297.1 , XM_029046298.1 , XM_029046295.1 , XM_029046299.1
Accession Version	XM_029046299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	uncharacterized protein LOC105221203 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863671.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_028902132.1
CDS	267..4751
Translation	MDSNLKNQTSNENPRGSVSSIKSLEVPPTPTRSPKKVSFSDDLPFSETPAATQTSTTTTTGAGNDSSEAHNHNNSELTAPSSVTEEELTAVNHVLLQAAQYLEQLHGARDRHPESYSPPPTSHNEISTSSKQINLRQPPQKVKRLDRAAGGAGAVPNVRAQEIDISDEFDKVQLHKLPNINATATTTVPISPTTIVAANIVSVSPAVLELDNNIEQATTTIAQQQQLHSPLSKTQSIVSANTDPSSASATRKTFTTSLSNVQLAEPTTSAQLEEITEEQSHVARYIDRYNLNLDKNSSAMEVEARREKLRWLLISECSALLAEGKHTREAFRKLVLEKEFQQKFFHLFDLEGNGYLVQDRWIEHLKGRLTPLDSTYADSCDDRQMDFAEQLESVAYVICGENSKITPKNFTEIWQARGILDKLYRLIDVDGTNLISTNQIMEFISHLTNSRPRTGFDKSSLVRLEQLFRSTVGNEREIRREEFQKIVTSKNPFFTDRVFQIFDKDNSGSISLQEFIDAIHQFSGQSADDKIRFLFKVYDIDGDGLIQHKELHDVIRACMDENGMEFSEEQIEDLTTAMFEDADPYNRGEITYEALKNQICKHGGLLENLSITIDRWLVPMTQENPKPKSKCPRLRCGKPHQLTLAYMKNNQAFVSYLYIYIAINVCLFISRAIQYRASNGFVIIARACGQCLNFNCAWILVLMLRHSLTYLRSQGLSSYLPLDNHIYLHKLTGIVVSILSLLHTIAHLFNFSIIVVNDPKINAGHYTIGEWLLTDRPGLFGLIPGCANPTGLALLIILFIMFICSQPFVRRKGSFEVFYWTHLLYIPFWILLLFHGPNFWKWFLVPGLVYFVERILRFVWMKSEHGKTYISSGLLLPSKVIHLVIKRPFNFNFRPGDYVFVNIPAIANYEWHPFTISSAPEQEDYMWLHIRTVGEWTNRLYQYFEKEQQKLQNGEIIQHRHAIPTDLVRITNKTETKTSATPQIDFLAQNLARLHGKPGANDSVLVPSKDKSPMQNVPLKPARHTQQPSKLAIENTNARMENDGGPTKHADRIRSIKKTLQRTFSRKDVPAKNETDGHWNEGFVGDQVDSAQKPLDKRLQKLHSRKPQLEKSLSLPDIAVKSKKKERLKALRALGRSESERSFDESRVRRARLQSGGLAYLSPQNKSLAQSFRYMRNKPTIIAFRTPSLEESEPDFTTTQRGSQDAYRELEEGKENGVDKTTGTVIEVSEATVSKPLEDPTSRTKVGRFRRPTFLRSLSTSIKNHGSNGSLPPQGHHNSGSKVTLDAGVLEIFIDGPYGAPSSHIFGAQHAVLIGTGIGVTPFASILQSIMHRYWKARHTCPRCHFEWASDIPKTVMNLRKVDFFWINRDQRSFEWFVNLLSQLEIEQAELGGPMERFLDMHMYITSALQRTDMKAVGLQLALDLLHEKGKRDLITGLKTRTNAGRPNWDKVFKQLQAQKKGKVTVFYCGPPQLGKTLRYKCDQYGFAFRKECF

Target ORF information:

RefSeq Version	XM_029046299.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae uncharacterized LOC105221203 (LOC105221203), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029046299.1

ORF Insert Sequence:

ATGGACTCTA ATCTCAAGAA TCAAACCTCT AATGAGAACC CCCGCGGCTC TGTGTCCTCC 
ATTAAATCGC TGGAGGTGCC GCCCACACCA ACGCGATCAC CCAAGAAGGT TTCATTCTCG 
GATGACCTGC CTTTCAGTGA AACACCCGCC GCGACGCAAA CCAGCACCAC CACTACAACC 
GGTGCAGGTA ATGACAGCAG CGAGGCCCAC AATCACAACA ACAGTGAATT AACAGCGCCC 
AGCTCTGTTA CCGAGGAGGA GCTAACCGCA GTAAATCATG TGCTGCTGCA GGCGGCACAA 
TATCTGGAGC AATTGCACGG CGCTCGAGAT CGCCATCCAG AGTCATATAG CCCACCTCCA 
ACGAGCCACA ATGAGATCAG CACAAGCAGC AAGCAAATAA ATCTTCGGCA GCCACCACAG 
AAAGTGAAGC GCCTCGACAG AGCTGCTGGC GGCGCTGGTG CAGTGCCAAA TGTGAGAGCG 
CAAGAAATAG ATATCAGTGA TGAATTTGAT AAGGTGCAAT TGCATAAATT ACCCAATATC 
AACGCAACTG CTACAACAAC AGTGCCAATA TCACCAACAA CAATAGTAGC AGCTAACATT 
GTTAGCGTAT CACCTGCTGT GCTTGAATTG GACAACAACA TTGAACAAGC GACAACAACG 
ATAGCACAGC AACAACAACT GCACAGCCCA CTAAGCAAAA CACAATCAAT AGTGTCTGCA 
AATACAGACC CGTCCTCCGC GTCAGCGACA AGGAAAACAT TTACCACCTC CCTCAGCAAC 
GTGCAGCTGG CGGAACCAAC TACCTCGGCA CAATTGGAAG AGATAACCGA AGAGCAATCT 
CACGTGGCCC GATACATTGA CCGCTACAAC CTCAATTTAG ATAAGAACTC CAGCGCGATG 
GAAGTGGAGG CAAGACGTGA AAAACTACGC TGGCTACTCA TATCCGAGTG CAGCGCACTG 
CTCGCCGAGG GCAAGCACAC TCGCGAGGCA TTCCGGAAAT TGGTGCTGGA AAAGGAATTC 
CAACAAAAGT TTTTCCATCT CTTCGATCTC GAAGGCAACG GTTATTTGGT GCAGGATCGT 
TGGATTGAAC ATTTGAAAGG TCGTCTAACT CCTTTGGACT CAACATACGC AGATTCTTGT 
GATGATAGAC AAATGGATTT CGCCGAACAA CTCGAGTCTG TGGCATATGT GATTTGTGGC 
GAAAATAGTA AGATCACACC GAAAAACTTT ACAGAAATTT GGCAAGCGCG TGGTATTCTG 
GATAAATTAT ACCGTCTGAT CGATGTCGAT GGCACAAATC TGATATCCAC AAATCAAATA 
ATGGAATTCA TTTCACACCT GACCAATTCA CGGCCACGCA CCGGTTTTGA TAAGAGCTCC 
CTGGTGCGTC TGGAGCAACT ATTTCGCAGC ACTGTAGGCA ATGAACGGGA GATTCGACGC 
GAAGAATTCC AGAAGATAGT CACATCGAAA AACCCATTCT TTACCGATCG TGTCTTTCAA 
ATTTTCGACA AGGATAACTC CGGTTCGATA TCATTGCAAG AGTTCATTGA TGCCATACAT 
CAATTCTCCG GCCAGTCAGC GGATGATAAA ATACGTTTCC TATTCAAAGT ATACGATATC 
GATGGTGACG GGCTGATACA GCATAAAGAA TTGCATGATG TGATACGCGC CTGCATGGAT 
GAAAATGGTA TGGAATTCTC CGAAGAACAA ATTGAAGATC TGACAACGGC TATGTTTGAA 
GATGCGGATC CATATAATCG TGGTGAAATC ACTTATGAAG CCTTAAAAAA TCAAATATGC 
AAACATGGTG GATTATTAGA GAATCTCAGT ATAACCATAG ATCGTTGGCT TGTGCCCATG 
ACGCAGGAGA ATCCGAAACC AAAGTCGAAA TGCCCGCGAT TGCGCTGTGG CAAGCCACAT 
CAACTCACTT TGGCCTACAT GAAAAACAAT CAAGCTTTCG TTTCGTATCT CTACATCTAC 
ATTGCCATAA ATGTGTGCCT CTTCATCTCA CGAGCCATTC AATATCGAGC GAGCAATGGT 
TTTGTGATCA TTGCGCGTGC TTGTGGACAA TGTTTGAATT TCAATTGCGC CTGGATATTA 
GTGCTCATGT TGCGACATTC ACTCACATAT CTGCGTTCAC AGGGTCTTTC GTCGTACTTA 
CCACTGGATA ACCACATATA TCTCCACAAA CTTACGGGCA TTGTCGTGTC AATTCTCAGT 
CTGCTTCACA CCATCGCACA TCTTTTTAAT TTCTCTATAA TTGTGGTGAA CGATCCGAAA 
ATCAATGCTG GTCACTACAC TATTGGCGAA TGGCTGCTAA CCGATCGTCC AGGGCTGTTC 
GGTCTCATTC CGGGCTGTGC CAACCCCACC GGTTTAGCTC TGCTAATCAT TCTATTTATA 
ATGTTCATTT GCTCGCAGCC ATTCGTACGC CGCAAGGGTT CCTTCGAAGT CTTCTATTGG 
ACTCATCTGC TTTATATACC ATTTTGGATT CTGCTACTGT TTCATGGTCC AAATTTCTGG 
AAATGGTTCT TGGTGCCAGG GCTGGTGTAC TTTGTGGAGC GTATATTGCG ATTTGTCTGG 
ATGAAAAGTG AACATGGTAA AACCTACATC AGTTCCGGTT TACTTTTACC ATCCAAAGTC 
ATACATTTGG TAATCAAACG TCCATTCAAC TTCAATTTCC GTCCTGGTGA TTATGTCTTT 
GTTAATATAC CGGCTATTGC CAACTATGAA TGGCATCCCT TCACAATTAG TTCAGCACCG 
GAACAAGAGG ATTACATGTG GCTGCACATA CGCACTGTAG GCGAGTGGAC CAATCGTTTG 
TACCAGTATT TCGAGAAGGA GCAGCAGAAA CTGCAAAATG GCGAAATAAT ACAGCATCGA 
CATGCGATAC CCACCGACCT TGTGCGTATT ACCAACAAAA CCGAAACAAA AACATCCGCA 
ACGCCGCAAA TCGATTTCCT TGCTCAAAAT CTAGCACGTT TACATGGTAA ACCCGGTGCG 
AATGATTCTG TATTAGTACC TAGTAAAGAC AAAAGTCCAA TGCAAAATGT TCCACTCAAA 
CCCGCGCGAC ACACACAGCA ACCTTCGAAA TTGGCCATTG AAAACACCAA TGCACGTATG 
GAGAACGACG GTGGGCCAAC AAAACACGCA GATCGTATAC GCAGTATTAA GAAAACACTA 
CAACGTACCT TCTCACGCAA AGATGTGCCT GCGAAGAATG AGACGGATGG CCACTGGAAT 
GAAGGTTTTG TAGGCGATCA AGTGGACTCA GCACAAAAGC CACTGGACAA ACGTTTACAG 
AAGTTACACT CACGTAAACC ACAGCTCGAA AAGAGTCTCT CGCTACCAGA TATAGCCGTC 
AAGTCTAAAA AGAAGGAACG GCTCAAAGCT TTACGAGCGC TCGGTCGTTC GGAATCGGAA 
AGGTCTTTCG ACGAATCACG TGTCCGTCGT GCGCGACTCC AGAGCGGAGG TTTAGCCTAT 
TTAAGTCCAC AAAATAAGTC GCTTGCACAG AGTTTTCGTT ACATGCGCAA TAAGCCGACA 
ATAATTGCCT TTAGAACACC GAGTCTAGAA GAATCCGAGC CAGATTTTAC AACAACACAA 
AGAGGCAGCC AGGATGCATA CAGAGAACTC GAAGAAGGAA AAGAAAATGG AGTAGACAAG 
ACCACCGGCA CGGTAATTGA GGTCTCCGAA GCCACCGTTT CCAAGCCGTT GGAAGATCCA 
ACATCGCGAA CTAAAGTTGG TCGTTTTCGC AGACCGACCT TCTTGCGTTC CCTCTCGACA 
TCCATCAAAA ATCACGGTAG CAATGGCAGC CTACCACCAC AGGGACACCA TAACTCTGGT 
AGCAAGGTTA CATTAGACGC TGGCGTATTA GAGATCTTCA TCGATGGACC GTATGGTGCG 
CCGTCGAGTC ATATATTTGG TGCTCAACAT GCTGTGCTCA TCGGCACTGG TATCGGTGTC 
ACGCCGTTCG CATCTATTTT GCAATCCATA ATGCATCGTT ACTGGAAGGC TCGACACACC 
TGTCCACGTT GTCACTTTGA GTGGGCTAGT GACATACCGA AAACGGTGAT GAATCTGCGT 
AAAGTGGACT TCTTTTGGAT CAACCGCGAT CAGCGCTCAT TCGAGTGGTT TGTTAATCTA 
TTATCACAGC TGGAAATCGA ACAGGCAGAG TTGGGCGGAC CAATGGAGAG ATTCCTCGAT 
ATGCACATGT ATATAACTAG TGCACTTCAA CGCACCGATA TGAAGGCAGT TGGACTACAG 
CTGGCTTTAG ATTTACTACA TGAAAAGGGC AAGCGTGATC TCATTACTGG TCTGAAGACA 
CGCACAAATG CCGGTCGTCC GAACTGGGAT AAAGTCTTTA AACAGCTGCA GGCGCAGAAG 
AAGGGCAAGG TGACGGTATT CTACTGTGGT CCACCGCAAC TTGGCAAAAC ACTACGCTAT 
AAATGCGATC AGTATGGCTT TGCATTCCGC AAAGAATGTT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LOC105221203
Entrez Gene ID	105221203
Full Name	uncharacterized LOC105221203
Synonyms	NOX5_2
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC105221203 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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