LOC105209336 cDNA ORF clone, Zeugodacus cucurbitae(melon fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105209336
Entrez Gene ID	105209336
Full Name	cytochrome P450 12b1, mitochondrial-like
Gene Type	protein-coding
Organism	Zeugodacus cucurbitae(melon fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011179700.2	XP_011178002.1	cytochrome P450 12b1, mitochondrial
XM_011179700.1	XP_011178002.1	cytochrome P450 12b1, mitochondrial-like

Pathways from BioSystems
Gene Ontology

The following LOC105209336 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105209336 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa195146	XM_011179700.2 Latest version!	Zeugodacus cucurbitae cytochrome P450 12b1, mitochondrial (Cyp12b1_1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OBa195146	XM_011179700.1	Bactrocera cucurbitae cytochrome P450 12b1, mitochondrial-like (LOC105209336), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105209336 ( XM_011179700.2 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011178002.1 Zeugodacus cucurbitae cytochrome P450 12b1, mitochondrial (Cyp12b1_1), mRNA.

Price & Availability↑

CloneID	OBa195146
Clone ID Related Accession (Same CDS sequence)	XM_011179700.2 , XM_011179700.1
Accession Version	XM_011179700.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-27
Organism	Zeugodacus cucurbitae(melon fly)
Product	cytochrome P450 12b1, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863678.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2019 this sequence version replaced XM_011179700.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Zeugodacus cucurbitae Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGA TGCGATATGC AGTTAAACAA ACCGGTCATG GGCTTGCTCG ACTTCCACAG 
CTCAGCTGTG CCGCTCAACA GGCGCAACAG CGGCGTACCG GCTTTTCGAG TGCGACCGAA 
CAACAATTAA GAGAACTCAT CGACGAAAAT GCACCACATC GCACGGATGC CGAATGGTTG 
AATGCCAAAG AGTACAACGC AATTCCCGGA CCATCTAAAT ATGGATTTTT GCTGAACTTT 
CTGCCTGGCG GTGAATTCTA TGAAATGAAC TATGTGGATA TGAATAAAAT ACTGCGCAAG 
CGTTACGGCG ACATATTTCT CTTGAAGGGT GTATTTAGTC GTCCCGACAC TATATTCACT 
TACAATGCGG CAGATTTTGA AGTGGTCTAT CGCAATGAGG GCATCTGGCC GATACGTGTG 
GCTCTCATGA GTTTTGATTA TTTTCGAAAA GTCAAACGTG TGGATTTCTT CAAGGGCTGC 
AGTGGACTTG TGACTGAACA TGGACGCAGT TGGGGTGATA TGCGCAATAA AGTGAATCCG 
GTTATGATGA AATCGACCAG CATACGTGAG GATCTGCCGG AAATCGATAA AATAACCATG 
AAGTTTGTCG ACAGACTTGA AACGTTGCGC GATCCGATCA CAGGACTCTT GAAAAACGAT 
TTTCATATTG AGCTTAAAAA GTGGGCCTTT GAGTCGGTCA CCTATATCGC GTTGAATAAA 
CGCCTAAATA TGTCTAGTGA AGAAGGTAAT AAGGAAGCAT CGGCGTTAGC ACAAAATTTA 
GCAGATTTCC TAAATCTAAG CTTTATCTAC GATATGACAC CCACCATGTG TAAATACTTC 
GAAACACCGG GGTTCAAGAA ACTAATGCGC GCCTACAACA GCATCACCGA GATCACTTAC 
AAGTATGTGC TCGAAGCGAC GAAACGCATC GAAAGTGAGC GCAATGCGGA AGCGAAGAGC 
GTGTTGGAAA AACTATTGCG CATCGATACA CATTACGCGA TGTTAATGAG CATCGATTCG 
TTAACCTCCG GCATGGACAC GACCGCTTCC ACTTTCATTA CAACGCTCTA TCAAATTGCG 
CGTAATCCCG AGAAGCAGCG TGCCTTGCGT CGTGAGTTAA TGCAAATTAT GCCCGATCCA 
CACACCCCTT TAAATGCACA GAACACCAAA CACATGCCAT ACTTACGCGC TTGCATCAAA 
GAGGCGCAGC GCATAACACC CATAGCGCCC GGTAATCTGC GCACAATGCC GAAAGACGTT 
GTGCTCTCCG GCTATCGCAT ACCGCGCGGC ACCTCGGTGC ACATGGGCAA TATGGCGCTC 
TGCAACAGCG AGAAATATTA TCCGCGTTAC AAAGAGTTCA TTCCAGAACG TTGGTTGAAA 
TCTGGCGAAA GTGCTTGCCC AGAATTGCGC AGCAGCAATC CGTTTATTTA TGCGCCCTTC 
GGATTTGGTC CTCGCACTTG TGTCGGTAAG CGCATAGCCG AAATGGAGTT GGAAACGCTG 
TTGGCGCGTC TGTTGCGTTT GTATCAAGTG AGCTGGGCCG CACCGGAGGA GATGCAGTAC 
ATAAGCAATC TCATTTTGAC ACCAAAGGCG CCCATGAAGT TCAGATTTAT GCCGCTCGAA 
GAGGAGGACT GCGCCATTGG CAGTTTCTCT GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011178002.1
CDS	197..1852
Translation	MWKMRYAVKQTGHGLARLPQLSCAAQQAQQRRTGFSSATEQQLRELIDENAPHRTDAEWLNAKEYNAIPGPSKYGFLLNFLPGGEFYEMNYVDMNKILRKRYGDIFLLKGVFSRPDTIFTYNAADFEVVYRNEGIWPIRVALMSFDYFRKVKRVDFFKGCSGLVTEHGRSWGDMRNKVNPVMMKSTSIREDLPEIDKITMKFVDRLETLRDPITGLLKNDFHIELKKWAFESVTYIALNKRLNMSSEEGNKEASALAQNLADFLNLSFIYDMTPTMCKYFETPGFKKLMRAYNSITEITYKYVLEATKRIESERNAEAKSVLEKLLRIDTHYAMLMSIDSLTSGMDTTASTFITTLYQIARNPEKQRALRRELMQIMPDPHTPLNAQNTKHMPYLRACIKEAQRITPIAPGNLRTMPKDVVLSGYRIPRGTSVHMGNMALCNSEKYYPRYKEFIPERWLKSGESACPELRSSNPFIYAPFGFGPRTCVGKRIAEMELETLLARLLRLYQVSWAAPEEMQYISNLILTPKAPMKFRFMPLEEEDCAIGSFSV

Target ORF information:

RefSeq Version	XM_011179700.2
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Zeugodacus cucurbitae cytochrome P450 12b1, mitochondrial (Cyp12b1_1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011179700.2

ORF Insert Sequence:

ATGTGGAAGA TGCGATATGC AGTTAAACAA ACCGGTCATG GGCTTGCTCG ACTTCCACAG 
CTCAGCTGTG CCGCTCAACA GGCGCAACAG CGGCGTACCG GCTTTTCGAG TGCGACCGAA 
CAACAATTAA GAGAACTCAT CGACGAAAAT GCACCACATC GCACGGATGC CGAATGGTTG 
AATGCCAAAG AGTACAACGC AATTCCCGGA CCATCTAAAT ATGGATTTTT GCTGAACTTT 
CTGCCTGGCG GTGAATTCTA TGAAATGAAC TATGTGGATA TGAATAAAAT ACTGCGCAAG 
CGTTACGGCG ACATATTTCT CTTGAAGGGT GTATTTAGTC GTCCCGACAC TATATTCACT 
TACAATGCGG CAGATTTTGA AGTGGTCTAT CGCAATGAGG GCATCTGGCC GATACGTGTG 
GCTCTCATGA GTTTTGATTA TTTTCGAAAA GTCAAACGTG TGGATTTCTT CAAGGGCTGC 
AGTGGACTTG TGACTGAACA TGGACGCAGT TGGGGTGATA TGCGCAATAA AGTGAATCCG 
GTTATGATGA AATCGACCAG CATACGTGAG GATCTGCCGG AAATCGATAA AATAACCATG 
AAGTTTGTCG ACAGACTTGA AACGTTGCGC GATCCGATCA CAGGACTCTT GAAAAACGAT 
TTTCATATTG AGCTTAAAAA GTGGGCCTTT GAGTCGGTCA CCTATATCGC GTTGAATAAA 
CGCCTAAATA TGTCTAGTGA AGAAGGTAAT AAGGAAGCAT CGGCGTTAGC ACAAAATTTA 
GCAGATTTCC TAAATCTAAG CTTTATCTAC GATATGACAC CCACCATGTG TAAATACTTC 
GAAACACCGG GGTTCAAGAA ACTAATGCGC GCCTACAACA GCATCACCGA GATCACTTAC 
AAGTATGTGC TCGAAGCGAC GAAACGCATC GAAAGTGAGC GCAATGCGGA AGCGAAGAGC 
GTGTTGGAAA AACTATTGCG CATCGATACA CATTACGCGA TGTTAATGAG CATCGATTCG 
TTAACCTCCG GCATGGACAC GACCGCTTCC ACTTTCATTA CAACGCTCTA TCAAATTGCG 
CGTAATCCCG AGAAGCAGCG TGCCTTGCGT CGTGAGTTAA TGCAAATTAT GCCCGATCCA 
CACACCCCTT TAAATGCACA GAACACCAAA CACATGCCAT ACTTACGCGC TTGCATCAAA 
GAGGCGCAGC GCATAACACC CATAGCGCCC GGTAATCTGC GCACAATGCC GAAAGACGTT 
GTGCTCTCCG GCTATCGCAT ACCGCGCGGC ACCTCGGTGC ACATGGGCAA TATGGCGCTC 
TGCAACAGCG AGAAATATTA TCCGCGTTAC AAAGAGTTCA TTCCAGAACG TTGGTTGAAA 
TCTGGCGAAA GTGCTTGCCC AGAATTGCGC AGCAGCAATC CGTTTATTTA TGCGCCCTTC 
GGATTTGGTC CTCGCACTTG TGTCGGTAAG CGCATAGCCG AAATGGAGTT GGAAACGCTG 
TTGGCGCGTC TGTTGCGTTT GTATCAAGTG AGCTGGGCCG CACCGGAGGA GATGCAGTAC 
ATAAGCAATC TCATTTTGAC ACCAAAGGCG CCCATGAAGT TCAGATTTAT GCCGCTCGAA 
GAGGAGGACT GCGCCATTGG CAGTTTCTCT GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105209336 ( XM_011179700.1 ) cDNA ORF clone, Zeugodacus cucurbitae(melon fly) -> XP_011178002.1 Bactrocera cucurbitae cytochrome P450 12b1, mitochondrial-like (LOC105209336), mRNA.

Price & Availability↑

CloneID	OBa195146
Clone ID Related Accession (Same CDS sequence)	XM_011179700.2 , XM_011179700.1
Accession Version	XM_011179700.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-28
Organism	Zeugodacus cucurbitae(melon fly)
Product	cytochrome P450 12b1, mitochondrial-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011863678.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera cucurbitae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGAAGA TGCGATATGC AGTTAAACAA ACCGGTCATG GGCTTGCTCG ACTTCCACAG 
CTCAGCTGTG CCGCTCAACA GGCGCAACAG CGGCGTACCG GCTTTTCGAG TGCGACCGAA 
CAACAATTAA GAGAACTCAT CGACGAAAAT GCACCACATC GCACGGATGC CGAATGGTTG 
AATGCCAAAG AGTACAACGC AATTCCCGGA CCATCTAAAT ATGGATTTTT GCTGAACTTT 
CTGCCTGGCG GTGAATTCTA TGAAATGAAC TATGTGGATA TGAATAAAAT ACTGCGCAAG 
CGTTACGGCG ACATATTTCT CTTGAAGGGT GTATTTAGTC GTCCCGACAC TATATTCACT 
TACAATGCGG CAGATTTTGA AGTGGTCTAT CGCAATGAGG GCATCTGGCC GATACGTGTG 
GCTCTCATGA GTTTTGATTA TTTTCGAAAA GTCAAACGTG TGGATTTCTT CAAGGGCTGC 
AGTGGACTTG TGACTGAACA TGGACGCAGT TGGGGTGATA TGCGCAATAA AGTGAATCCG 
GTTATGATGA AATCGACCAG CATACGTGAG GATCTGCCGG AAATCGATAA AATAACCATG 
AAGTTTGTCG ACAGACTTGA AACGTTGCGC GATCCGATCA CAGGACTCTT GAAAAACGAT 
TTTCATATTG AGCTTAAAAA GTGGGCCTTT GAGTCGGTCA CCTATATCGC GTTGAATAAA 
CGCCTAAATA TGTCTAGTGA AGAAGGTAAT AAGGAAGCAT CGGCGTTAGC ACAAAATTTA 
GCAGATTTCC TAAATCTAAG CTTTATCTAC GATATGACAC CCACCATGTG TAAATACTTC 
GAAACACCGG GGTTCAAGAA ACTAATGCGC GCCTACAACA GCATCACCGA GATCACTTAC 
AAGTATGTGC TCGAAGCGAC GAAACGCATC GAAAGTGAGC GCAATGCGGA AGCGAAGAGC 
GTGTTGGAAA AACTATTGCG CATCGATACA CATTACGCGA TGTTAATGAG CATCGATTCG 
TTAACCTCCG GCATGGACAC GACCGCTTCC ACTTTCATTA CAACGCTCTA TCAAATTGCG 
CGTAATCCCG AGAAGCAGCG TGCCTTGCGT CGTGAGTTAA TGCAAATTAT GCCCGATCCA 
CACACCCCTT TAAATGCACA GAACACCAAA CACATGCCAT ACTTACGCGC TTGCATCAAA 
GAGGCGCAGC GCATAACACC CATAGCGCCC GGTAATCTGC GCACAATGCC GAAAGACGTT 
GTGCTCTCCG GCTATCGCAT ACCGCGCGGC ACCTCGGTGC ACATGGGCAA TATGGCGCTC 
TGCAACAGCG AGAAATATTA TCCGCGTTAC AAAGAGTTCA TTCCAGAACG TTGGTTGAAA 
TCTGGCGAAA GTGCTTGCCC AGAATTGCGC AGCAGCAATC CGTTTATTTA TGCGCCCTTC 
GGATTTGGTC CTCGCACTTG TGTCGGTAAG CGCATAGCCG AAATGGAGTT GGAAACGCTG 
TTGGCGCGTC TGTTGCGTTT GTATCAAGTG AGCTGGGCCG CACCGGAGGA GATGCAGTAC 
ATAAGCAATC TCATTTTGAC ACCAAAGGCG CCCATGAAGT TCAGATTTAT GCCGCTCGAA 
GAGGAGGACT GCGCCATTGG CAGTTTCTCT GTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011178002.1
CDS	210..1865
Translation	MWKMRYAVKQTGHGLARLPQLSCAAQQAQQRRTGFSSATEQQLRELIDENAPHRTDAEWLNAKEYNAIPGPSKYGFLLNFLPGGEFYEMNYVDMNKILRKRYGDIFLLKGVFSRPDTIFTYNAADFEVVYRNEGIWPIRVALMSFDYFRKVKRVDFFKGCSGLVTEHGRSWGDMRNKVNPVMMKSTSIREDLPEIDKITMKFVDRLETLRDPITGLLKNDFHIELKKWAFESVTYIALNKRLNMSSEEGNKEASALAQNLADFLNLSFIYDMTPTMCKYFETPGFKKLMRAYNSITEITYKYVLEATKRIESERNAEAKSVLEKLLRIDTHYAMLMSIDSLTSGMDTTASTFITTLYQIARNPEKQRALRRELMQIMPDPHTPLNAQNTKHMPYLRACIKEAQRITPIAPGNLRTMPKDVVLSGYRIPRGTSVHMGNMALCNSEKYYPRYKEFIPERWLKSGESACPELRSSNPFIYAPFGFGPRTCVGKRIAEMELETLLARLLRLYQVSWAAPEEMQYISNLILTPKAPMKFRFMPLEEEDCAIGSFSV

Target ORF information:

RefSeq Version	XM_011179700.1
Organism	Zeugodacus cucurbitae(melon fly)
Definition	Bactrocera cucurbitae cytochrome P450 12b1, mitochondrial-like (LOC105209336), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011179700.1

ORF Insert Sequence:

ATGTGGAAGA TGCGATATGC AGTTAAACAA ACCGGTCATG GGCTTGCTCG ACTTCCACAG 
CTCAGCTGTG CCGCTCAACA GGCGCAACAG CGGCGTACCG GCTTTTCGAG TGCGACCGAA 
CAACAATTAA GAGAACTCAT CGACGAAAAT GCACCACATC GCACGGATGC CGAATGGTTG 
AATGCCAAAG AGTACAACGC AATTCCCGGA CCATCTAAAT ATGGATTTTT GCTGAACTTT 
CTGCCTGGCG GTGAATTCTA TGAAATGAAC TATGTGGATA TGAATAAAAT ACTGCGCAAG 
CGTTACGGCG ACATATTTCT CTTGAAGGGT GTATTTAGTC GTCCCGACAC TATATTCACT 
TACAATGCGG CAGATTTTGA AGTGGTCTAT CGCAATGAGG GCATCTGGCC GATACGTGTG 
GCTCTCATGA GTTTTGATTA TTTTCGAAAA GTCAAACGTG TGGATTTCTT CAAGGGCTGC 
AGTGGACTTG TGACTGAACA TGGACGCAGT TGGGGTGATA TGCGCAATAA AGTGAATCCG 
GTTATGATGA AATCGACCAG CATACGTGAG GATCTGCCGG AAATCGATAA AATAACCATG 
AAGTTTGTCG ACAGACTTGA AACGTTGCGC GATCCGATCA CAGGACTCTT GAAAAACGAT 
TTTCATATTG AGCTTAAAAA GTGGGCCTTT GAGTCGGTCA CCTATATCGC GTTGAATAAA 
CGCCTAAATA TGTCTAGTGA AGAAGGTAAT AAGGAAGCAT CGGCGTTAGC ACAAAATTTA 
GCAGATTTCC TAAATCTAAG CTTTATCTAC GATATGACAC CCACCATGTG TAAATACTTC 
GAAACACCGG GGTTCAAGAA ACTAATGCGC GCCTACAACA GCATCACCGA GATCACTTAC 
AAGTATGTGC TCGAAGCGAC GAAACGCATC GAAAGTGAGC GCAATGCGGA AGCGAAGAGC 
GTGTTGGAAA AACTATTGCG CATCGATACA CATTACGCGA TGTTAATGAG CATCGATTCG 
TTAACCTCCG GCATGGACAC GACCGCTTCC ACTTTCATTA CAACGCTCTA TCAAATTGCG 
CGTAATCCCG AGAAGCAGCG TGCCTTGCGT CGTGAGTTAA TGCAAATTAT GCCCGATCCA 
CACACCCCTT TAAATGCACA GAACACCAAA CACATGCCAT ACTTACGCGC TTGCATCAAA 
GAGGCGCAGC GCATAACACC CATAGCGCCC GGTAATCTGC GCACAATGCC GAAAGACGTT 
GTGCTCTCCG GCTATCGCAT ACCGCGCGGC ACCTCGGTGC ACATGGGCAA TATGGCGCTC 
TGCAACAGCG AGAAATATTA TCCGCGTTAC AAAGAGTTCA TTCCAGAACG TTGGTTGAAA 
TCTGGCGAAA GTGCTTGCCC AGAATTGCGC AGCAGCAATC CGTTTATTTA TGCGCCCTTC 
GGATTTGGTC CTCGCACTTG TGTCGGTAAG CGCATAGCCG AAATGGAGTT GGAAACGCTG 
TTGGCGCGTC TGTTGCGTTT GTATCAAGTG AGCTGGGCCG CACCGGAGGA GATGCAGTAC 
ATAAGCAATC TCATTTTGAC ACCAAAGGCG CCCATGAAGT TCAGATTTAT GCCGCTCGAA 
GAGGAGGACT GCGCCATTGG CAGTTTCTCT GTATAA