ddx28 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ddx28
Entrez Gene ID	100487122
Full Name	DEAD-box helicase 28
General protein information	Preferred Names DEAD-box helicase 28 Names probable ATP-dependent RNA helicase DDX28 DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002940974.5	XP_002941020.2	probable ATP-dependent RNA helicase DDX28
XM_002940974.4	XP_002941020.1	probable ATP-dependent RNA helicase DDX28

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx28	NP_082314.2
Drosophila melanogaster (fruit fly)	KH1	NP_608544.1
Xenopus tropicalis (tropical clawed frog)	ddx28	XP_002941020.1
Homo sapiens (human)	DDX28	NP_060850.2
Gallus gallus (chicken)	DDX28	XP_425116.4
Danio rerio (zebrafish)	ddx28	NP_001070800.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013035	XP_003436418.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012856	XP_306103.4
Arabidopsis thaliana (thale cress)	RH39	NP_849348.1
Oryza sativa (rice)	Os01g0184500	NP_001042234.1
Canis lupus familiaris (dog)	DDX28	XP_546867.1
Rattus norvegicus (Norway rat)	Ddx28	NP_001102368.1
Pan troglodytes (chimpanzee)	DDX28	XP_511048.2
	DDX28	XP_001097528.1
Bos taurus (cattle)	DDX28	NP_001098892.1

Gene Ontology

The following ddx28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ddx28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OXa08780	XM_002940974.4 Latest version!	Xenopus tropicalis DEAD-box helicase 28 (ddx28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OXa56774	XM_002940974.5 Latest version!	Xenopus tropicalis DEAD-box helicase 28 (ddx28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ddx28 ( XM_002940974.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941020.1 Xenopus tropicalis DEAD-box helicase 28 (ddx28), mRNA.

Price & Availability↑

CloneID	OXa08780
Clone ID Related Accession (Same CDS sequence)	XM_002940974.4
Accession Version	XM_002940974.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002940974.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAACG TAAGAAACGG CGTGTTTCGA CCTGCTTTTC ACTGGTATTT TAATTACCGG 
AGTTATTGTG TTAACTTGCC AGTAATACGT CTTCCTTGTC GTTCTCAACT ACGTCTTGAT 
CATTTAAGAA AGCAACCATC GGCTCCATCT GTGTTACCTA CCCGAACAGG GAAACTTCTG 
GTCCTTAGCA AAAGAAGGGA ACTGAACCAG TATGCTGCTG AGACCATCGG GCGATGGGAG 
AATCGCCCAT TGGTGAGTCA AGGATGGAAA CATAATGCAT CACGTGGTGA TCATTTTATC 
ATTTTACAGC AACAAGGACA TGGGCCAACA CCTGACATCT CCTGCAACTT TCAGTCTTTG 
GGATTGGATG AAGAACTGGT ATCAATACTT AATTCCATAG GCATCACCAG TCCTACCTGG 
GTGCAAATAA AGTCAATCCG TAATCTTCTT CAAGGAAAAA ATATTCTCAG TACAGCTGAA 
ACGGGCAGTG GCAAAACACT TGCGTACCTT TTGCCCATTT TGCATGAACT CAAACGTTCC 
AAACGCTCAG CTCATTCCTC ATTCCCCCGT TGCTTGGTTT TGGTGCCATC AAGGGAGCTT 
GCTGGTCAGA TTGTGTCAGT AGCTCAAAAC TTTTGTGCCA AGTTACAATT GACTGTACAA 
TTTGTTGGGG GTGGATTAGG CCAAACAGCA GTGGAAAGCA AACTAGGTAG GGGTCCAATA 
GATATTTTGG TAGCTACACC TGGGGCACTC TGGAAAGCTT TGCAAAAAGA CATTATTAGT 
CTGAACGAAC TTTGTTATGT GGTATTGGAT GAAGTAGATA CCCTGATCGA TCATACCTTT 
CTAAAACTGA CTGGTGATAT TCTTAAGCAC ATAAAGATAG CTTCCAGTCC CAGTGACTTG 
CAGGGTTCTG AAAAAATGGC TCAGCTTGCG GTTATTGGAG CCACTTTCCC TAATGGCACT 
GATCAGGTCC TAGGTAAAAT AACGGGTCTA GAAAATATTA CGACAATTAA AAGCAGCAAG 
GTGCACTTCC CACTGTTGCA TGTCCAGCAG ACTTTCCTTA GAGTTAAAGG GTCAGAAAAA 
GTATTGGAAC TACTTACGTT GTTGAAGAAG CAACAAGTTG GGAGTCCTGG GTCTGGGGTT 
TTCATCTTTT GCAACAGTTC TAGTACTGTG AATTGGCTGA GTTACATCCT AGAGGAACAT 
GGCATGAAGC ACTACCGACT ACATGGTCAA ATGCCAGCCA GAATGCGAAT GAACATCTAT 
AAATCTTTTC AGAAAGGACA AAAGGAAGCG CTGGTTTGCA CAGACATAGC CTCCCGGGGC 
CTGGACATTT CTAGAGTGAA CACAGTGGTG AATTATGACT TCCCTTTTAC TCTTCAAGAC 
TACTTGCATA GAGTGGGCAG AGTGGGACGT GTAGGTAGTG GGGAACACTG TTCTGCTGTT 
AGCTTTGTTA CACATGCTTG GGATGTGGAA CTGGTGCAGA AAATTGAAAC AGCAGTTCGT 
AAACATATTA TGCTTCCTTG CATGTATATC AATACAAAAG AAAATCTACA AAAAGAACTT 
GTAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941020.1
CDS	78..1646
Translation	MLNVRNGVFRPAFHWYFNYRSYCVNLPVIRLPCRSQLRLDHLRKQPSAPSVLPTRTGKLLVLSKRRELNQYAAETIGRWENRPLVSQGWKHNASRGDHFIILQQQGHGPTPDISCNFQSLGLDEELVSILNSIGITSPTWVQIKSIRNLLQGKNILSTAETGSGKTLAYLLPILHELKRSKRSAHSSFPRCLVLVPSRELAGQIVSVAQNFCAKLQLTVQFVGGGLGQTAVESKLGRGPIDILVATPGALWKALQKDIISLNELCYVVLDEVDTLIDHTFLKLTGDILKHIKIASSPSDLQGSEKMAQLAVIGATFPNGTDQVLGKITGLENITTIKSSKVHFPLLHVQQTFLRVKGSEKVLELLTLLKKQQVGSPGSGVFIFCNSSSTVNWLSYILEEHGMKHYRLHGQMPARMRMNIYKSFQKGQKEALVCTDIASRGLDISRVNTVVNYDFPFTLQDYLHRVGRVGRVGSGEHCSAVSFVTHAWDVELVQKIETAVRKHIMLPCMYINTKENLQKELVK

Target ORF information:

RefSeq Version	XM_002940974.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 28 (ddx28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940974.4

ORF Insert Sequence:

ATGTTAAACG TAAGAAACGG CGTGTTTCGA CCTGCTTTTC ACTGGTATTT TAATTACCGG 
AGTTATTGTG TTAACTTGCC AGTAATACGT CTTCCTTGTC GTTCTCAACT ACGTCTTGAT 
CATTTAAGAA AGCAACCATC GGCTCCATCT GTGTTACCTA CCCGAACAGG GAAACTTCTG 
GTCCTTAGCA AAAGAAGGGA ACTGAACCAG TATGCTGCTG AGACCATCGG GCGATGGGAG 
AATCGCCCAT TGGTGAGTCA AGGATGGAAA CATAATGCAT CACGTGGTGA TCATTTTATC 
ATTTTACAGC AACAAGGACA TGGGCCAACA CCTGACATCT CCTGCAACTT TCAGTCTTTG 
GGATTGGATG AAGAACTGGT ATCAATACTT AATTCCATAG GCATCACCAG TCCTACCTGG 
GTGCAAATAA AGTCAATCCG TAATCTTCTT CAAGGAAAAA ATATTCTCAG TACAGCTGAA 
ACGGGCAGTG GCAAAACACT TGCGTACCTT TTGCCCATTT TGCATGAACT CAAACGTTCC 
AAACGCTCAG CTCATTCCTC ATTCCCCCGT TGCTTGGTTT TGGTGCCATC AAGGGAGCTT 
GCTGGTCAGA TTGTGTCAGT AGCTCAAAAC TTTTGTGCCA AGTTACAATT GACTGTACAA 
TTTGTTGGGG GTGGATTAGG CCAAACAGCA GTGGAAAGCA AACTAGGTAG GGGTCCAATA 
GATATTTTGG TAGCTACACC TGGGGCACTC TGGAAAGCTT TGCAAAAAGA CATTATTAGT 
CTGAACGAAC TTTGTTATGT GGTATTGGAT GAAGTAGATA CCCTGATCGA TCATACCTTT 
CTAAAACTGA CTGGTGATAT TCTTAAGCAC ATAAAGATAG CTTCCAGTCC CAGTGACTTG 
CAGGGTTCTG AAAAAATGGC TCAGCTTGCG GTTATTGGAG CCACTTTCCC TAATGGCACT 
GATCAGGTCC TAGGTAAAAT AACGGGTCTA GAAAATATTA CGACAATTAA AAGCAGCAAG 
GTGCACTTCC CACTGTTGCA TGTCCAGCAG ACTTTCCTTA GAGTTAAAGG GTCAGAAAAA 
GTATTGGAAC TACTTACGTT GTTGAAGAAG CAACAAGTTG GGAGTCCTGG GTCTGGGGTT 
TTCATCTTTT GCAACAGTTC TAGTACTGTG AATTGGCTGA GTTACATCCT AGAGGAACAT 
GGCATGAAGC ACTACCGACT ACATGGTCAA ATGCCAGCCA GAATGCGAAT GAACATCTAT 
AAATCTTTTC AGAAAGGACA AAAGGAAGCG CTGGTTTGCA CAGACATAGC CTCCCGGGGC 
CTGGACATTT CTAGAGTGAA CACAGTGGTG AATTATGACT TCCCTTTTAC TCTTCAAGAC 
TACTTGCATA GAGTGGGCAG AGTGGGACGT GTAGGTAGTG GGGAACACTG TTCTGCTGTT 
AGCTTTGTTA CACATGCTTG GGATGTGGAA CTGGTGCAGA AAATTGAAAC AGCAGTTCGT 
AAACATATTA TGCTTCCTTG CATGTATATC AATACAAAAG AAAATCTACA AAAAGAACTT 
GTAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ddx28 ( XM_002940974.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002941020.2 Xenopus tropicalis DEAD-box helicase 28 (ddx28), mRNA.

Price & Availability↑

CloneID	OXa56774
Clone ID Related Accession (Same CDS sequence)	XM_002940974.5
Accession Version	XM_002940974.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	probable ATP-dependent RNA helicase DDX28
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030680.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002940974.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAACG TAAGAAACGG CGTGTTTCGA CCTGCTTTTC ATTGGTATTT TAATTACCGG 
AGTTATTGTG TTAACTTGCC AGTAATACGT CTTCCTTGTC GTTCTCAACT ACGTCTTGAT 
CATTTAAGAA AGCAACCATC GGCGCCATCT GTGTTACCTA CCCGAACAGG GAAACTTCTG 
GTCCTTAGCA AAAGAAGGGA ACTGAACCAG TATGCTGCTG AGACCATCGG GCGATGGGAG 
AATCGCCCAT TGGTGAGTCA AGGATGGAAA CATAATGCGT CACGTGGTGA TCATTTTATC 
ATTTTACAGC AACAAGGACA TGGGCCAACA CCTGACATCT CCTGCAACTT TCAGTCTTTG 
GGATTGGATG AAGAACTGGT ATCAATACTT AATTCCATAG GCATCACCAG TCCTACCTGG 
GTGCAAATAA AGTCAATCCG TAATCTTCTT CAAGGAAAAA ATATTCTCAG TACAGCTGAA 
ACGGGCAGTG GCAAAACACT TGCGTACCTT TTGCCCATTT TGCATGAACT CAAACGTTCC 
AAACGCTCAG CTCATTCCTC ACTCCCCCGT TGCTTGGTTT TGGTGCCATC AAGGGAGCTT 
GCTGGTCAGA TTGTGTCAGT AGCTCAAAAC TTTTGTGCCA AGTTACAATT GACTGTACAA 
TTTGTTGGGG GTGGATTAGG CCAAACAGCA GTGGAAAGCA AACTAGGTAG GGGTCCAATA 
GATATTTTGG TAGCTACACC TGGGGCACTC TGGAAAGCTT TGCAAAAAGA CATTATTAGT 
CTGAACGAAC TTTGTTATGT GGTATTGGAT GAAGTAGATA CCCTGATCGA TCATACCTTT 
CTAAAACTGA CTGGTGATAT TCTTAAGCAC ATAAAGATAG CTTCCAGTCC CAGTGACTTG 
CAGGGTTCTG AAAAAATGGC TCAGCTTGCG GTTATTGGAG CCACTTTCCC TAATGGCACT 
GATCAGGTCC TAGGTAAAAT AACGGGTCTA GAAAATATTA CGACAATTAA AAGCAGCAAG 
GTGCACTTCC CACTGTTGCA TGTCCAGCAG ACTTTCCTTA GAGTTAAAGG GTCAGAAAAA 
GTATTGGAAC TACTTACGTT GTTGAAGAAG CAACAACTTG GGAGTCCTGG GTCTGGGGTT 
TTCATCTTTT GCAACAGTTC TAGTACTGTG AATTGGCTGA GTTACATCCT AGAGGAACAT 
GGCATGAAGC ACTACCGACT ACATGGTCAA ATGCCAGCCA GAATGCGAAT GAACATCTAT 
AAATCTTTTC AGAAAGGACA AAAGGAAGTG CTGGTTTGCA CAGACATAGC CTCCCGGGGC 
CTGGACATTT CTAGAGTGAA CACAGTGGTG AATTATGACT TCCCTTTTAC TCTTCAAGAC 
TACTTGCATA GAGTGGGCAG AGTGGGACGT GTAGGTAGTG GGGAACACTG TTCTGCTGTT 
AGCTTTGTTA CACATGCTTG GGATGTGGAA CTGGTGCAGA AAATTGAAAC AGCAGTTCGT 
AAACATATTA TGCTTCCTTG CATGTATATC AATACAAAAG AAAATCTACA AAAAGAACTT 
GTAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002941020.2
CDS	86..1654
Translation	MLNVRNGVFRPAFHWYFNYRSYCVNLPVIRLPCRSQLRLDHLRKQPSAPSVLPTRTGKLLVLSKRRELNQYAAETIGRWENRPLVSQGWKHNASRGDHFIILQQQGHGPTPDISCNFQSLGLDEELVSILNSIGITSPTWVQIKSIRNLLQGKNILSTAETGSGKTLAYLLPILHELKRSKRSAHSSLPRCLVLVPSRELAGQIVSVAQNFCAKLQLTVQFVGGGLGQTAVESKLGRGPIDILVATPGALWKALQKDIISLNELCYVVLDEVDTLIDHTFLKLTGDILKHIKIASSPSDLQGSEKMAQLAVIGATFPNGTDQVLGKITGLENITTIKSSKVHFPLLHVQQTFLRVKGSEKVLELLTLLKKQQLGSPGSGVFIFCNSSSTVNWLSYILEEHGMKHYRLHGQMPARMRMNIYKSFQKGQKEVLVCTDIASRGLDISRVNTVVNYDFPFTLQDYLHRVGRVGRVGSGEHCSAVSFVTHAWDVELVQKIETAVRKHIMLPCMYINTKENLQKELVK

Target ORF information:

RefSeq Version	XM_002940974.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis DEAD-box helicase 28 (ddx28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002940974.5

ORF Insert Sequence:

ATGTTAAACG TAAGAAACGG CGTGTTTCGA CCTGCTTTTC ATTGGTATTT TAATTACCGG 
AGTTATTGTG TTAACTTGCC AGTAATACGT CTTCCTTGTC GTTCTCAACT ACGTCTTGAT 
CATTTAAGAA AGCAACCATC GGCGCCATCT GTGTTACCTA CCCGAACAGG GAAACTTCTG 
GTCCTTAGCA AAAGAAGGGA ACTGAACCAG TATGCTGCTG AGACCATCGG GCGATGGGAG 
AATCGCCCAT TGGTGAGTCA AGGATGGAAA CATAATGCGT CACGTGGTGA TCATTTTATC 
ATTTTACAGC AACAAGGACA TGGGCCAACA CCTGACATCT CCTGCAACTT TCAGTCTTTG 
GGATTGGATG AAGAACTGGT ATCAATACTT AATTCCATAG GCATCACCAG TCCTACCTGG 
GTGCAAATAA AGTCAATCCG TAATCTTCTT CAAGGAAAAA ATATTCTCAG TACAGCTGAA 
ACGGGCAGTG GCAAAACACT TGCGTACCTT TTGCCCATTT TGCATGAACT CAAACGTTCC 
AAACGCTCAG CTCATTCCTC ACTCCCCCGT TGCTTGGTTT TGGTGCCATC AAGGGAGCTT 
GCTGGTCAGA TTGTGTCAGT AGCTCAAAAC TTTTGTGCCA AGTTACAATT GACTGTACAA 
TTTGTTGGGG GTGGATTAGG CCAAACAGCA GTGGAAAGCA AACTAGGTAG GGGTCCAATA 
GATATTTTGG TAGCTACACC TGGGGCACTC TGGAAAGCTT TGCAAAAAGA CATTATTAGT 
CTGAACGAAC TTTGTTATGT GGTATTGGAT GAAGTAGATA CCCTGATCGA TCATACCTTT 
CTAAAACTGA CTGGTGATAT TCTTAAGCAC ATAAAGATAG CTTCCAGTCC CAGTGACTTG 
CAGGGTTCTG AAAAAATGGC TCAGCTTGCG GTTATTGGAG CCACTTTCCC TAATGGCACT 
GATCAGGTCC TAGGTAAAAT AACGGGTCTA GAAAATATTA CGACAATTAA AAGCAGCAAG 
GTGCACTTCC CACTGTTGCA TGTCCAGCAG ACTTTCCTTA GAGTTAAAGG GTCAGAAAAA 
GTATTGGAAC TACTTACGTT GTTGAAGAAG CAACAACTTG GGAGTCCTGG GTCTGGGGTT 
TTCATCTTTT GCAACAGTTC TAGTACTGTG AATTGGCTGA GTTACATCCT AGAGGAACAT 
GGCATGAAGC ACTACCGACT ACATGGTCAA ATGCCAGCCA GAATGCGAAT GAACATCTAT 
AAATCTTTTC AGAAAGGACA AAAGGAAGTG CTGGTTTGCA CAGACATAGC CTCCCGGGGC 
CTGGACATTT CTAGAGTGAA CACAGTGGTG AATTATGACT TCCCTTTTAC TCTTCAAGAC 
TACTTGCATA GAGTGGGCAG AGTGGGACGT GTAGGTAGTG GGGAACACTG TTCTGCTGTT 
AGCTTTGTTA CACATGCTTG GGATGTGGAA CTGGTGCAGA AAATTGAAAC AGCAGTTCGT 
AAACATATTA TGCTTCCTTG CATGTATATC AATACAAAAG AAAATCTACA AAAAGAACTT 
GTAAAATGA