Ddx28 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddx28
Entrez Gene ID	71986
Full Name	DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
Synonyms	2410004K13Rik,AI449652,Mddx28
General protein information	Preferred Names DEAD (Asp-Glu-Ala-Asp) box polypeptide 28 Names probable ATP-dependent RNA helicase DDX28 ATP-dependent RNA helicase Ddx28 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 28 mitochondrial DEAD box protein 28
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 8 Map Location: 8\|8 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_028038.3	NP_082314.2	probable ATP-dependent RNA helicase DDX28

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Ddx28	NP_082314.2
Drosophila melanogaster (fruit fly)	KH1	NP_608544.1
Xenopus tropicalis (tropical clawed frog)	ddx28	XP_002941020.1
Homo sapiens (human)	DDX28	NP_060850.2
Gallus gallus (chicken)	DDX28	XP_425116.4
Danio rerio (zebrafish)	ddx28	NP_001070800.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP013035	XP_003436418.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012856	XP_306103.4
Arabidopsis thaliana (thale cress)	RH39	NP_849348.1
Oryza sativa (rice)	Os01g0184500	NP_001042234.1
Canis lupus familiaris (dog)	DDX28	XP_546867.1
Rattus norvegicus (Norway rat)	Ddx28	NP_001102368.1
Pan troglodytes (chimpanzee)	DDX28	XP_511048.2
	DDX28	XP_001097528.1
Bos taurus (cattle)	DDX28	NP_001098892.1

Gene Ontology
Bibliography

Related articles in PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, Magen A, Canidio E, Pagani M, Peluso I, Lin-Marq N, Koch M, Bilio M, Cantiello I, Verde R, De Masi C, Bianchi SA, Cicchini J, Perroud E, Mehmeti S, Dagand E, Schrinner S, N?rnberger A, Schmidt K, Metz K, Zwingmann C, Brieske N, Springer C, Hernandez AM, Herzog S, Grabbe F, Sieverding C, Fischer B, Schrader K, Brockmeyer M, Dettmer S, Helbig C, Alunni V, Battaini MA, Mura C, Henrichsen CN, Garcia-Lopez R, Echevarria D, Puelles E, Garcia-Calero E, Kruse S, Uhr M, Kauck C, Feng G, Milyaev N, Ong CK, Kumar L, Lam M, Semple CA, Gyenesei A, Mundlos S, Radelof U, Lehrach H, Sarmientos P, Reymond A, Davidson DR, Doll? P, Antonarakis SE, Yaspo ML, Martinez S, Baldock RA, Eichele G, Ballabio A
PLoS biology9(1)e1000582(2011 Jan)

A mitochondrial protein compendium elucidates complex I disease biology.
Pagliarini DJ, Calvo SE, Chang B, Sheth SA, Vafai SB, Ong SE, Walford GA, Sugiana C, Boneh A, Chen WK, Hill DE, Vidal M, Evans JG, Thorburn DR, Carr SA, Mootha VK
Cell134(1)112-23(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ddx28 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddx28 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu58586	NM_028038.3 Latest version!	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 28 (Ddx28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddx28 ( NM_028038.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_082314.2 Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 28 (Ddx28), mRNA.

Price & Availability↑

CloneID	OMu58586
Clone ID Related Accession (Same CDS sequence)	NM_028038.3
Accession Version	NM_028038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	probable ATP-dependent RNA helicase DDX28
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC120582.1, AK163480.1 and AC133499.3. On Aug 4, 2009 this sequence version replaced NM_028038.2. ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTAG CCGGGCCATC GCGGCTGCTG GCGTTAGCAG TACGGTTGCT TCTGGAGCCT 
CGACGGAACC TAGTGGTGCG CGGTTCCGAC CAGTCCTTGC CAGTGGTGCG CGTTCCGCGG 
GCTCTGCAGC GACGGCAGGA ACAGCGACAG AGCGGCCGTG GGAGTTTGCA GCGGCCAGTG 
CTAGTGCGCC CCGGTCCCCT GCTGGTCTCG GCGCGGCGGC CGGAGTTGAA CCAGCCCGCA 
CGCCTCACGC TGGGCCGTTG GGAGCGAGCG CCACTGGCTT CCCGTGGCTG GAAGCATCGG 
CGATCGCGGC AAGACCATTT CTCCATAGAG CGAGTGCAGC AAGAGGCGCC CGCCCTGAGG 
AACCTCTCGT CCCGGGGCAG CTTCGTGGAC CTGGGTCTGG AACCCCGAGT GCTGCTGGCG 
CTTCAGGAGG CTGTCCCCGA AGTTGTGCAG CCAACCTCAG TGCAGTCGAA AACCATCCCC 
CCACTGCTTC GAGGCCGCCA CCTCCTTTGC GCTGCGGAAA CTGGCAGTGG CAAGACGCTT 
AGCTACCTAC TGCCCCTATT TCAGCGGCTC CTGAGAGGGT CAGACCTGGA CTCCCGCAGT 
TTCACGGCTC CTCGAGGCTT GGTTCTGGTA CCTTCCCGAG AACTAGCCGA ACAGGTGCAG 
GCAGTGGCCC AGTCACTGGG TGGGTACTTG GGCCTGCAGG TGATAGAACT TGGCGGAGGC 
CTCGGGATGA GTAGGCTCAA ACTTCAGCTG TATAGACGAC CGGCAGCCGA TGTGTTAGTG 
GCTACTCCAG GGGCGCTGTG GAAGGCTCTG AAAAGTCAAC TGATCAGCTT ACAGCATCTT 
AACTTCATAG TGCTAGATGA AGTAGACACA CTGCTAGATG AAAGCTTCCT GGAACTGGTG 
GACTACATCT TGGAAAAGAG CCCTATAGCA GAAAGTCCAG CCGAGTTAGA AGACCCCTTT 
AATCCCAAAG CCCAGTTAGT GCTGGTGGGA GCCACGTTTC CAGAAGGCCT AAACCAGTTA 
CTGAGTAAAG TCACCAGTCC AGACAGTCTG ACCACCATTA CCAGCTCCAA GCTGCACTGT 
CTCATGCCTC ATGTCAGACA GACATTTATG AGACTGAAGG GAGCAGACAA GGTGACAGAA 
CTGGTTCAGA TCCTCAAACA GCAGGACAAA GCAAGTAAGA CGGAACCCTC GGGAACTGTC 
CTGGTATTCT GCAACAGCGC CAGCACCGTG AACTGGCTTG GGTACATTCT GGATGACCAC 
AAGATTCAAC ATCTAAGGCT GCAAGGGCAA ATGCCGGCCT CAATGAGGGC AGGTATCTTC 
CAGAGTTTCC AGAAGGGCTC CCAGAACATA CTTGTCTGCA CAGACATCGC CTCCCGAGGC 
CTAGACAGTG TCCACGTGGA AGTGGTTATC AATTACGATT TCCCCCCTAC CCTGCAGGAT 
TACATCCACA GAGCAGGAAG GGTGGGTCGT GTGGGCAGCG AAGTGCCAGG GTCCGTTATC 
AGTTTTGTGA CCCACCCCTG GGATGTGAGC CTGGTTCAGA AAATTGAACT GGCAGCTCGC 
CGAAGGAGAA GCCTTCCAGG ACTAGCATCT TCAGTGGGGG ACCCTTTGCC TCAAAAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_082314.2
CDS	63..1685
Translation	MALAGPSRLLALAVRLLLEPRRNLVVRGSDQSLPVVRVPRALQRRQEQRQSGRGSLQRPVLVRPGPLLVSARRPELNQPARLTLGRWERAPLASRGWKHRRSRQDHFSIERVQQEAPALRNLSSRGSFVDLGLEPRVLLALQEAVPEVVQPTSVQSKTIPPLLRGRHLLCAAETGSGKTLSYLLPLFQRLLRGSDLDSRSFTAPRGLVLVPSRELAEQVQAVAQSLGGYLGLQVIELGGGLGMSRLKLQLYRRPAADVLVATPGALWKALKSQLISLQHLNFIVLDEVDTLLDESFLELVDYILEKSPIAESPAELEDPFNPKAQLVLVGATFPEGLNQLLSKVTSPDSLTTITSSKLHCLMPHVRQTFMRLKGADKVTELVQILKQQDKASKTEPSGTVLVFCNSASTVNWLGYILDDHKIQHLRLQGQMPASMRAGIFQSFQKGSQNILVCTDIASRGLDSVHVEVVINYDFPPTLQDYIHRAGRVGRVGSEVPGSVISFVTHPWDVSLVQKIELAARRRRSLPGLASSVGDPLPQKA

Target ORF information:

RefSeq Version	NM_028038.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus DEAD (Asp-Glu-Ala-Asp) box polypeptide 28 (Ddx28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_028038.3

ORF Insert Sequence:

ATGGCTTTAG CCGGGCCATC GCGGCTGCTG GCGTTAGCAG TACGGTTGCT TCTGGAGCCT 
CGACGGAACC TAGTGGTGCG CGGTTCCGAC CAGTCCTTGC CAGTGGTGCG CGTTCCGCGG 
GCTCTGCAGC GACGGCAGGA ACAGCGACAG AGCGGCCGTG GGAGTTTGCA GCGGCCAGTG 
CTAGTGCGCC CCGGTCCCCT GCTGGTCTCG GCGCGGCGGC CGGAGTTGAA CCAGCCCGCA 
CGCCTCACGC TGGGCCGTTG GGAGCGAGCG CCACTGGCTT CCCGTGGCTG GAAGCATCGG 
CGATCGCGGC AAGACCATTT CTCCATAGAG CGAGTGCAGC AAGAGGCGCC CGCCCTGAGG 
AACCTCTCGT CCCGGGGCAG CTTCGTGGAC CTGGGTCTGG AACCCCGAGT GCTGCTGGCG 
CTTCAGGAGG CTGTCCCCGA AGTTGTGCAG CCAACCTCAG TGCAGTCGAA AACCATCCCC 
CCACTGCTTC GAGGCCGCCA CCTCCTTTGC GCTGCGGAAA CTGGCAGTGG CAAGACGCTT 
AGCTACCTAC TGCCCCTATT TCAGCGGCTC CTGAGAGGGT CAGACCTGGA CTCCCGCAGT 
TTCACGGCTC CTCGAGGCTT GGTTCTGGTA CCTTCCCGAG AACTAGCCGA ACAGGTGCAG 
GCAGTGGCCC AGTCACTGGG TGGGTACTTG GGCCTGCAGG TGATAGAACT TGGCGGAGGC 
CTCGGGATGA GTAGGCTCAA ACTTCAGCTG TATAGACGAC CGGCAGCCGA TGTGTTAGTG 
GCTACTCCAG GGGCGCTGTG GAAGGCTCTG AAAAGTCAAC TGATCAGCTT ACAGCATCTT 
AACTTCATAG TGCTAGATGA AGTAGACACA CTGCTAGATG AAAGCTTCCT GGAACTGGTG 
GACTACATCT TGGAAAAGAG CCCTATAGCA GAAAGTCCAG CCGAGTTAGA AGACCCCTTT 
AATCCCAAAG CCCAGTTAGT GCTGGTGGGA GCCACGTTTC CAGAAGGCCT AAACCAGTTA 
CTGAGTAAAG TCACCAGTCC AGACAGTCTG ACCACCATTA CCAGCTCCAA GCTGCACTGT 
CTCATGCCTC ATGTCAGACA GACATTTATG AGACTGAAGG GAGCAGACAA GGTGACAGAA 
CTGGTTCAGA TCCTCAAACA GCAGGACAAA GCAAGTAAGA CGGAACCCTC GGGAACTGTC 
CTGGTATTCT GCAACAGCGC CAGCACCGTG AACTGGCTTG GGTACATTCT GGATGACCAC 
AAGATTCAAC ATCTAAGGCT GCAAGGGCAA ATGCCGGCCT CAATGAGGGC AGGTATCTTC 
CAGAGTTTCC AGAAGGGCTC CCAGAACATA CTTGTCTGCA CAGACATCGC CTCCCGAGGC 
CTAGACAGTG TCCACGTGGA AGTGGTTATC AATTACGATT TCCCCCCTAC CCTGCAGGAT 
TACATCCACA GAGCAGGAAG GGTGGGTCGT GTGGGCAGCG AAGTGCCAGG GTCCGTTATC 
AGTTTTGTGA CCCACCCCTG GGATGTGAGC CTGGTTCAGA AAATTGAACT GGCAGCTCGC 
CGAAGGAGAA GCCTTCCAGG ACTAGCATCT TCAGTGGGGG ACCCTTTGCC TCAAAAGGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

A high-resolution anatomical atlas of the transcriptome in the mouse embryo.
PLoS biology9(1)e1000582(2011 Jan)
Diez-Roux G,Banfi S,Sultan M,Geffers L,Anand S,Rozado D,Magen A,Canidio E,Pagani M,Peluso I,Lin-Marq N,Koch M,Bilio M,Cantiello I,Verde R,De Masi C,Bianchi SA,Cicchini J,Perroud E,Mehmeti S,Dagand E,Schrinner S,N?rnberger A,Schmidt K,Metz K,Zwingmann C,Brieske N,Springer C,Hernandez AM,Herzog S,Grabbe F,Sieverding C,Fischer B,Schrader K,Brockmeyer M,Dettmer S,Helbig C,Alunni V,Battaini MA,Mura C,Henrichsen CN,Garcia-Lopez R,Echevarria D,Puelles E,Garcia-Calero E,Kruse S,Uhr M,Kauck C,Feng G,Milyaev N,Ong CK,Kumar L,Lam M,Semple CA,Gyenesei A,Mundlos S,Radelof U,Lehrach H,Sarmientos P,Reymond A,Davidson DR,Doll? P,Antonarakis SE,Yaspo ML,Martinez S,Baldock RA,Eichele G,Ballabio A

A mitochondrial protein compendium elucidates complex I disease biology.
Cell134(1)112-23(2008 Jul)
Pagliarini DJ,Calvo SE,Chang B,Sheth SA,Vafai SB,Ong SE,Walford GA,Sugiana C,Boneh A,Chen WK,Hill DE,Vidal M,Evans JG,Thorburn DR,Carr SA,Mootha VK

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Ddx28 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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