grem1.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	grem1.L
Entrez Gene ID	399094
Full Name	gremlin 1, DAN family BMP antagonist L homeolog
Synonyms	cktsf1b1,grem1
General protein information	Preferred Names gremlin 1, DAN family BMP antagonist L homeolog Names gremlin-1 cysteine knot superfamily 1, BMP antagonist 1
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001090277.1	NP_001083746.1	gremlin-1 precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gremlin1 induces anterior-posterior limb bifurcations in developing Xenopus limbs but does not enhance limb regeneration.
Wang YH, Keenan SR, Lynn J, McEwan JC, Beck CW
Mechanisms of development138 Pt 3256-67(2015 Nov)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

The Xenopus dorsalizing factor Gremlin identifies a novel family of secreted proteins that antagonize BMP activities.
Hsu DR, Economides AN, Wang X, Eimon PM, Harland RM
Molecular cell1(5)673-83(1998 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

both the regenerating and contralateral, developing limb of grem1 transgenics developed skeletal defects, suggesting that overexpressing grem1 negatively affects limb patterning
Title: Gremlin1 induces anterior-posterior limb bifurcations in developing Xenopus limbs but does not enhance limb regeneration.

The following grem1.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the grem1.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf01251	NM_001090277.1 Latest version!	Xenopus laevis gremlin 1, DAN family BMP antagonist L homeolog (grem1.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

grem1.L ( NM_001090277.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001083746.1 Xenopus laevis gremlin 1, DAN family BMP antagonist L homeolog (grem1.L), mRNA.

Price & Availability↑

CloneID	OAf01251
Clone ID Related Accession (Same CDS sequence)	NM_001090277.1
Accession Version	NM_001090277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Xenopus laevis(African clawed frog)
Product	gremlin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF045798.1. On Jul 25, 2007 this sequence version replaced NM_001090278.1. ##Evidence-Data-START## Transcript exon combination :: AF045798.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00012418, SAMD00012419 [ECO:0000348] ##Evidence-Data-END##

ATGAACTGTC TCGTTTATGC TCTAGGATCC CTTTTCCTTC TGAGTGGGCT CCTCCTCCCC 
AGCTCCGAAG GGAAGAAGAA GGTTAGTGGA TCACAGGGAG CCATTCCTCC CCCTGACAAA 
GGGCAGCCCA ATGACTCTGA GCAAGGTCAA GCTCAGCCAG GAGACCGGGT CAGAGGGAAG 
GGGAAGGGAC AAGCACTAGC GGCTGAAGAA GTGCTGGAGT CCAGTCAAGA AGCTCTACAC 
GTCACTGAAC GCAAGTATCT AAAGAGGGAC TGGTGTAAGA CTCAGCCCTT AAAGCAAACA 
ATTCACGAGG ACGGGTGCAA CAGTCGTACC ATCATCAACC GCTTCTGCTA TGGGCAATGC 
AACTCCTTCT ACATCCCCCG TCACATACGC AGGGAGGAGG GCTCATTTCA ATCCTGCTCC 
TTCTGCAAGC CTAAAAAATT CACCACAATG GTGGTCACCC TGAACTGCCC AGAGCTACAA 
CCTCCCACAA AGAAGAAGAG AATTACACGT GTCAAGCAGT GTCGCTGCAT CTCCATAGAC 
CTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001083746.1
CDS	66..614
Translation	MNCLVYALGSLFLLSGLLLPSSEGKKKVSGSQGAIPPPDKGQPNDSEQGQAQPGDRVRGKGKGQALAAEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEDGCNSRTIINRFCYGQCNSFYIPRHIRREEGSFQSCSFCKPKKFTTMVVTLNCPELQPPTKKKRITRVKQCRCISIDLD

Target ORF information:

RefSeq Version	NM_001090277.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis gremlin 1, DAN family BMP antagonist L homeolog (grem1.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001090277.1

ORF Insert Sequence:

ATGAACTGTC TCGTTTATGC TCTAGGATCC CTTTTCCTTC TGAGTGGGCT CCTCCTCCCC 
AGCTCCGAAG GGAAGAAGAA GGTTAGTGGA TCACAGGGAG CCATTCCTCC CCCTGACAAA 
GGGCAGCCCA ATGACTCTGA GCAAGGTCAA GCTCAGCCAG GAGACCGGGT CAGAGGGAAG 
GGGAAGGGAC AAGCACTAGC GGCTGAAGAA GTGCTGGAGT CCAGTCAAGA AGCTCTACAC 
GTCACTGAAC GCAAGTATCT AAAGAGGGAC TGGTGTAAGA CTCAGCCCTT AAAGCAAACA 
ATTCACGAGG ACGGGTGCAA CAGTCGTACC ATCATCAACC GCTTCTGCTA TGGGCAATGC 
AACTCCTTCT ACATCCCCCG TCACATACGC AGGGAGGAGG GCTCATTTCA ATCCTGCTCC 
TTCTGCAAGC CTAAAAAATT CACCACAATG GTGGTCACCC TGAACTGCCC AGAGCTACAA 
CCTCCCACAA AGAAGAAGAG AATTACACGT GTCAAGCAGT GTCGCTGCAT CTCCATAGAC 
CTGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gremlin1 induces anterior-posterior limb bifurcations in developing Xenopus limbs but does not enhance limb regeneration.
Mechanisms of development138 Pt 3256-67(2015 Nov)
Wang YH,Keenan SR,Lynn J,McEwan JC,Beck CW

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

The Xenopus dorsalizing factor Gremlin identifies a novel family of secreted proteins that antagonize BMP activities.
Molecular cell1(5)673-83(1998 Apr)
Hsu DR,Economides AN,Wang X,Eimon PM,Harland RM