lefty.L cDNA ORF clone, Xenopus laevis(African clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	lefty.L
Entrez Gene ID	373829
Full Name	left-right determination factor L homeolog
Synonyms	XATV,Xlefty,antivin,atv,leftb,lefty,lefty-1,lefty-a,lefty-b,lefty1,leftyb,lft-a
General protein information	Preferred Names left-right determination factor L homeolog Names left-right determination factor L homeolog TGF-beta family member lefty-A
Gene Type	protein-coding
Organism	Xenopus laevis(African clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001085745.1	NP_001079214.1	left-right determination factor L homeolog precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus.
Marjoram L, Wright C
Development (Cambridge, England)138(3)475-85(2011 Feb)

Cooperative non-cell and cell autonomous regulation of Nodal gene expression and signaling by Lefty/Antivin and Brachyury in Xenopus.
Cha YR, Takahashi S, Wright CV
Developmental biology290(2)246-64(2006 Feb)

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

Regulation of gut and heart left-right asymmetry by context-dependent interactions between xenopus lefty and BMP4 signaling.
Branford WW, Essner JJ, Yost HJ
Developmental biology223(2)291-306(2000 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus.
Title: Rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus.

there is a two-level suppressive mechanism for restricting the level, range, and duration of Xnr signaling via extracellular inhibition by Xatv/Xlefty coupled with potent indirect transcriptional repression by Xbra
Title: Cooperative non-cell and cell autonomous regulation of Nodal gene expression and signaling by Lefty/Antivin and Brachyury in Xenopus.

The following lefty.L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lefty.L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAf08957	NM_001085745.1 Latest version!	Xenopus laevis left-right determination factor L homeolog (lefty.L), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lefty.L ( NM_001085745.1 ) cDNA ORF clone, Xenopus laevis(African clawed frog) -> NP_001079214.1 Xenopus laevis left-right determination factor L homeolog (lefty.L), mRNA.

Price & Availability↑

CloneID	OAf08957
Clone ID Related Accession (Same CDS sequence)	NM_001085745.1
Accession Version	NM_001085745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1101bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-27
Organism	Xenopus laevis(African clawed frog)
Product	left-right determination factor L homeolog precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF283562.1. ##Evidence-Data-START## Transcript exon combination :: AF283562.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04157058 [ECO:0000348] ##Evidence-Data-END##

ATGGGTGTCA CTACCAAATC TTTGAGGCTC TGCTTGATCT TCAGCACCCT GTGCCTGGCT 
GCCATCAATG GCTTCAACCC TGACAACATC AGAGATGCTC TGCTCAAAAA ACTCAACCTC 
CAAGAGGTTC CCAAACTGGA GAAAAGAGAC GTTGAGAACC TGGTGATCCC TAGGCACATC 
CAAGCCAAAT ACATGTCTAT GCTGCACAGT CACAGAGAGA GGAAGAGGAG ATCACTGCCC 
AGCTTGGCTG GCATCCTCAG GGGCATCTCA GGCAATGCAG ATATTTCTGG GGAGATTCTA 
TACTCAGACT CCAGCAAGCA GACCATGGTC TTTGGAATGG AGTCCCGTAT TCCAGAGAAC 
AGTGAGGTGA CCATGGCTGA ACTCAAGCTC TTCAAGAAAC CCCCTAAAAT CATGAACGTC 
CCAGAGAGGA GATTCCACCG ACCAGTCAGC AATGCCAGAG TCAGTGTGTA TTATGTAGAG 
ATATTGAAAG ATGGGACCAA CAGAACCTCC CTGGTGGACT CTAGGCTTGT GCCAATTATG 
GAGTCAGGCT GGAGGAGCTT TGATGTGACC CAGGCTGTGC ATTACTGGAT GAAAAGTGGC 
GGACATTCAT CGATGCACTT AGAGATCCAC GTGGATGGTG AACGACATGG AAGTCATGCA 
TCTGAGATGG CAAAAATGGT TCGTTTCACC ACCCAGAGCC CATCAGACAA CTCTCTGGGA 
AAACCAGAAC TTGTCTTGTT CACTCTCAAC CTTGATGAGC ATGGGGCCCG TGGAGACTGT 
TCAGCATCAG GTGCTAAAAA AGACAACATC TGCTGCAGAG AGGAATATTT CATTAACTTT 
CGGGAGCTTA CCTGGACACA GTACTGGATT ATTGAGCCAG CAGGATATAA TGCTTTTCGG 
TGCGCAGGAA GTTGCAAGCA ACCAAAGTAC CCCTTGTCTC ATCACTATGG AGAGAGAATG 
TGCGCCGTGG TGGAAAGTGC CCCACTGCCC GTTATGTACC TGGTCAAAAA GGGAGACTAC 
ACGGAAATCG AAGTGGCAGA ATTCCCCAAT ATGATTGTGG AAAAATGCGG TTGCACAATG 
GACAATATCG CTATAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001079214.1
CDS	64..1164
Translation	MGVTTKSLRLCLIFSTLCLAAINGFNPDNIRDALLKKLNLQEVPKLEKRDVENLVIPRHIQAKYMSMLHSHRERKRRSLPSLAGILRGISGNADISGEILYSDSSKQTMVFGMESRIPENSEVTMAELKLFKKPPKIMNVPERRFHRPVSNARVSVYYVEILKDGTNRTSLVDSRLVPIMESGWRSFDVTQAVHYWMKSGGHSSMHLEIHVDGERHGSHASEMAKMVRFTTQSPSDNSLGKPELVLFTLNLDEHGARGDCSASGAKKDNICCREEYFINFRELTWTQYWIIEPAGYNAFRCAGSCKQPKYPLSHHYGERMCAVVESAPLPVMYLVKKGDYTEIEVAEFPNMIVEKCGCTMDNIAII

Target ORF information:

RefSeq Version	NM_001085745.1
Organism	Xenopus laevis(African clawed frog)
Definition	Xenopus laevis left-right determination factor L homeolog (lefty.L), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085745.1

ORF Insert Sequence:

ATGGGTGTCA CTACCAAATC TTTGAGGCTC TGCTTGATCT TCAGCACCCT GTGCCTGGCT 
GCCATCAATG GCTTCAACCC TGACAACATC AGAGATGCTC TGCTCAAAAA ACTCAACCTC 
CAAGAGGTTC CCAAACTGGA GAAAAGAGAC GTTGAGAACC TGGTGATCCC TAGGCACATC 
CAAGCCAAAT ACATGTCTAT GCTGCACAGT CACAGAGAGA GGAAGAGGAG ATCACTGCCC 
AGCTTGGCTG GCATCCTCAG GGGCATCTCA GGCAATGCAG ATATTTCTGG GGAGATTCTA 
TACTCAGACT CCAGCAAGCA GACCATGGTC TTTGGAATGG AGTCCCGTAT TCCAGAGAAC 
AGTGAGGTGA CCATGGCTGA ACTCAAGCTC TTCAAGAAAC CCCCTAAAAT CATGAACGTC 
CCAGAGAGGA GATTCCACCG ACCAGTCAGC AATGCCAGAG TCAGTGTGTA TTATGTAGAG 
ATATTGAAAG ATGGGACCAA CAGAACCTCC CTGGTGGACT CTAGGCTTGT GCCAATTATG 
GAGTCAGGCT GGAGGAGCTT TGATGTGACC CAGGCTGTGC ATTACTGGAT GAAAAGTGGC 
GGACATTCAT CGATGCACTT AGAGATCCAC GTGGATGGTG AACGACATGG AAGTCATGCA 
TCTGAGATGG CAAAAATGGT TCGTTTCACC ACCCAGAGCC CATCAGACAA CTCTCTGGGA 
AAACCAGAAC TTGTCTTGTT CACTCTCAAC CTTGATGAGC ATGGGGCCCG TGGAGACTGT 
TCAGCATCAG GTGCTAAAAA AGACAACATC TGCTGCAGAG AGGAATATTT CATTAACTTT 
CGGGAGCTTA CCTGGACACA GTACTGGATT ATTGAGCCAG CAGGATATAA TGCTTTTCGG 
TGCGCAGGAA GTTGCAAGCA ACCAAAGTAC CCCTTGTCTC ATCACTATGG AGAGAGAATG 
TGCGCCGTGG TGGAAAGTGC CCCACTGCCC GTTATGTACC TGGTCAAAAA GGGAGACTAC 
ACGGAAATCG AAGTGGCAGA ATTCCCCAAT ATGATTGTGG AAAAATGCGG TTGCACAATG 
GACAATATCG CTATAATATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rapid differential transport of Nodal and Lefty on sulfated proteoglycan-rich extracellular matrix regulates left-right asymmetry in Xenopus.
Development (Cambridge, England)138(3)475-85(2011 Feb)
Marjoram L,Wright C

Cooperative non-cell and cell autonomous regulation of Nodal gene expression and signaling by Lefty/Antivin and Brachyury in Xenopus.
Developmental biology290(2)246-64(2006 Feb)
Cha YR,Takahashi S,Wright CV

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

Regulation of gut and heart left-right asymmetry by context-dependent interactions between xenopus lefty and BMP4 signaling.
Developmental biology223(2)291-306(2000 Jul)
Branford WW,Essner JJ,Yost HJ