GCLC cDNA ORF clone, Tyto alba(Barn owl)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCLC
Entrez Gene ID	104358808
Full Name	glutamate-cysteine ligase catalytic subunit
Gene Type	protein-coding
Organism	Tyto alba(Barn owl)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009964463.1	XP_009962765.1	glutamate--cysteine ligase catalytic subunit

Pathways from BioSystems
Gene Ontology

The following GCLC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTy00277	XM_009964463.1 Latest version!	Tyto alba glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCLC ( XM_009964463.1 ) cDNA ORF clone, Tyto alba(Barn owl) -> XP_009962765.1 Tyto alba glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.

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CloneID	OTy00277
Clone ID Related Accession (Same CDS sequence)	XM_009964463.1
Accession Version	XM_009964463.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-30
Organism	Tyto alba(Barn owl)
Product	glutamate--cysteine ligase catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009972614.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tyto alba Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGTGG TTAATAGCTT GCATAGGGTT TTAATTTTTT TTTGTCTGAA AAATAATGCA 
GAGGTGGAAT ATATGTTAGT AAAATTTGAC CATGAGAATA AGAAAGTGCG CTTGGTGCTC 
AGTGGTGAAG AAGTTCTTCA AACACTGCAA GACAAGGGGG AGAAGATAAA CCCCAACCAC 
CCAACGCTCT GGCGACCAGA ATATGGAAGC TATATGATTG AAGGGACGCC TGGGCAGCCG 
TATGGAGGAA CCATGTCTGA ATTCAACACT GTGCAGGACA ACATGAGGAA AAGACGGCAA 
GAGGCCGCTT CTGTGCTCAA AGAAAATGAG GCTGTTTGCA CTGTGACGTC ATTTCCGAGG 
TTAGGGTGTC CTGGGTTTAC GGTGCCAGAA TATAAGCCAA CGCCAATAGA AGGAGGAGCT 
TCAAAATCCC TCTTTTTTCC AGATGAAGCC ATCAATAAAC ATCCTAGATT TAGTACACTG 
ACCAGGAACA TTCGGCACCG CAGAGGAGAG AAGGTTGTGA TAAATGTACC AATATTTAAA 
GATAAGAACA CACCATCACC GTTTATTGAA ACGTTTCCTA ATGATGATGG AGAAGCAGCA 
AAGGCGGCTA AACCAGACTA TATATACATG GATGCTATGG GTTTTGGAAT GGGAAACTGT 
TGTCTTCAGG TAACATTTCA GGCTTGCAGC ATTTCTGAAG CAAGGTATCT CTATGATCAG 
CTAGCCACGA TCTGTCCCAT TGTGATGGCG TTGAGTGCTG CATCTCCATT CTACAGAGGC 
TACGTGTCTG ATATTGACTG TCGTTGGGGA GTCATCTCTG CATCTGTAGA TGATCGAACG 
CGAGAAGAGA GGGGGCTAGA GCCACTGAAG AACAACCATT ATAGAATCAG TAAATCCAGA 
TATGATTCCA TAGACAGTTA TCTATCTGAA TGTGGTGAGA AATACAATGA CATTGATTTG 
ACAATAGACA AAGATATCTA CGAGCACCTA ATAAAGGAAG GTATTGACCA CCTTTTGGCA 
CAGCATATTG CACACTTGTT CATTCGAGAC CCATTGACTT TGTTTGAGGA GAAAATACAT 
CTAGACGATG CAAATGAATC CGACCATTTT GAGAATATTC AGTCTACGAA CTGGCAGACA 
ATGAGGTTTA AGCCACCTCC TCCAAATTCA GATATTGGAT GGAGAGTAGA ATTCAGACCT 
ATGGAGGTCC AGTTAACAGA CTTTGAAAAC TCTGCATATG TTGTGTTCGT GGTGTTGCTA 
ACCAGAGTGA TTCTGTCATA TAAACTGGAT TTTCTCATTC CTTTGTCCAA GGTGGATGAA 
AACATGAAGG TGGCCCAGAA AAGAGATGCT GTCCGGCAGG GAATGTTTTA CTTCAGAAAA 
GATATTTGCA AAGGTGGAAA CACTGTTGTG GATGGATGTG GCTCTGCACA GAACGGGACG 
GGCACAGACA CAGAAGAGTA CAGCTTAATG AGCATTGACA CAATTATCAA TGGGAAGGAA 
GGAGTCTTTC CTGGTTTGAT CCCAATTTTG AATTCCTATC TTGAAAACAT GGAAGTGGAT 
GTTGACACAA GGTGCACCAT CCTAAACTAC TTGAAGCTAA TAAAAAAGAG AGCATCTGGA 
GAATTAATGA CAGTTGCTCG ATGGATGAGG GAATTTATCG CACAGCATCC AGACTACAAG 
CAAGATAGCG TGATAACTGA TGAAATTAAT TATAGCCTTA TTTGGAGATG CAACCAAATT 
GCACAAGGCC AGGCAGAGTG TCCTGAACTG TTGGGGGTAG GATTTAATAA GAAACAAAGT 
GGAAACAAAA CTGGTTCACT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009962765.1
CDS	1..1824
Translation	MFVVNSLHRVLIFFCLKNNAEVEYMLVKFDHENKKVRLVLSGEEVLQTLQDKGEKINPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVQDNMRKRRQEAASVLKENEAVCTVTSFPRLGCPGFTVPEYKPTPIEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPSPFIETFPNDDGEAAKAAKPDYIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTIDKDIYEHLIKEGIDHLLAQHIAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVRQGMFYFRKDICKGGNTVVDGCGSAQNGTGTDTEEYSLMSIDTIINGKEGVFPGLIPILNSYLENMEVDVDTRCTILNYLKLIKKRASGELMTVARWMREFIAQHPDYKQDSVITDEINYSLIWRCNQIAQGQAECPELLGVGFNKKQSGNKTGSL

Target ORF information:

RefSeq Version	XM_009964463.1
Organism	Tyto alba(Barn owl)
Definition	Tyto alba glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009964463.1

ORF Insert Sequence:

ATGTTTGTGG TTAATAGCTT GCATAGGGTT TTAATTTTTT TTTGTCTGAA AAATAATGCA 
GAGGTGGAAT ATATGTTAGT AAAATTTGAC CATGAGAATA AGAAAGTGCG CTTGGTGCTC 
AGTGGTGAAG AAGTTCTTCA AACACTGCAA GACAAGGGGG AGAAGATAAA CCCCAACCAC 
CCAACGCTCT GGCGACCAGA ATATGGAAGC TATATGATTG AAGGGACGCC TGGGCAGCCG 
TATGGAGGAA CCATGTCTGA ATTCAACACT GTGCAGGACA ACATGAGGAA AAGACGGCAA 
GAGGCCGCTT CTGTGCTCAA AGAAAATGAG GCTGTTTGCA CTGTGACGTC ATTTCCGAGG 
TTAGGGTGTC CTGGGTTTAC GGTGCCAGAA TATAAGCCAA CGCCAATAGA AGGAGGAGCT 
TCAAAATCCC TCTTTTTTCC AGATGAAGCC ATCAATAAAC ATCCTAGATT TAGTACACTG 
ACCAGGAACA TTCGGCACCG CAGAGGAGAG AAGGTTGTGA TAAATGTACC AATATTTAAA 
GATAAGAACA CACCATCACC GTTTATTGAA ACGTTTCCTA ATGATGATGG AGAAGCAGCA 
AAGGCGGCTA AACCAGACTA TATATACATG GATGCTATGG GTTTTGGAAT GGGAAACTGT 
TGTCTTCAGG TAACATTTCA GGCTTGCAGC ATTTCTGAAG CAAGGTATCT CTATGATCAG 
CTAGCCACGA TCTGTCCCAT TGTGATGGCG TTGAGTGCTG CATCTCCATT CTACAGAGGC 
TACGTGTCTG ATATTGACTG TCGTTGGGGA GTCATCTCTG CATCTGTAGA TGATCGAACG 
CGAGAAGAGA GGGGGCTAGA GCCACTGAAG AACAACCATT ATAGAATCAG TAAATCCAGA 
TATGATTCCA TAGACAGTTA TCTATCTGAA TGTGGTGAGA AATACAATGA CATTGATTTG 
ACAATAGACA AAGATATCTA CGAGCACCTA ATAAAGGAAG GTATTGACCA CCTTTTGGCA 
CAGCATATTG CACACTTGTT CATTCGAGAC CCATTGACTT TGTTTGAGGA GAAAATACAT 
CTAGACGATG CAAATGAATC CGACCATTTT GAGAATATTC AGTCTACGAA CTGGCAGACA 
ATGAGGTTTA AGCCACCTCC TCCAAATTCA GATATTGGAT GGAGAGTAGA ATTCAGACCT 
ATGGAGGTCC AGTTAACAGA CTTTGAAAAC TCTGCATATG TTGTGTTCGT GGTGTTGCTA 
ACCAGAGTGA TTCTGTCATA TAAACTGGAT TTTCTCATTC CTTTGTCCAA GGTGGATGAA 
AACATGAAGG TGGCCCAGAA AAGAGATGCT GTCCGGCAGG GAATGTTTTA CTTCAGAAAA 
GATATTTGCA AAGGTGGAAA CACTGTTGTG GATGGATGTG GCTCTGCACA GAACGGGACG 
GGCACAGACA CAGAAGAGTA CAGCTTAATG AGCATTGACA CAATTATCAA TGGGAAGGAA 
GGAGTCTTTC CTGGTTTGAT CCCAATTTTG AATTCCTATC TTGAAAACAT GGAAGTGGAT 
GTTGACACAA GGTGCACCAT CCTAAACTAC TTGAAGCTAA TAAAAAAGAG AGCATCTGGA 
GAATTAATGA CAGTTGCTCG ATGGATGAGG GAATTTATCG CACAGCATCC AGACTACAAG 
CAAGATAGCG TGATAACTGA TGAAATTAAT TATAGCCTTA TTTGGAGATG CAACCAAATT 
GCACAAGGCC AGGCAGAGTG TCCTGAACTG TTGGGGGTAG GATTTAATAA GAAACAAAGT 
GGAAACAAAA CTGGTTCACT GTAA