SPG7 cDNA ORF clone, Tinamus guttatus(white-throated tinamou)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPG7
Entrez Gene ID	104572862
Full Name	SPG7, paraplegin matrix AAA peptidase subunit
Synonyms	Paraplegin
Gene Type	protein-coding
Organism	Tinamus guttatus(white-throated tinamou)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010220031.1	XP_010218333.1	paraplegin

Pathways from BioSystems
Gene Ontology

The following SPG7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPG7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTi21199	XM_010220031.1 Latest version!	Tinamus guttatus spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPG7 ( XM_010220031.1 ) cDNA ORF clone, Tinamus guttatus(white-throated tinamou) -> XP_010218333.1 Tinamus guttatus spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), mRNA.

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CloneID	OTi21199
Clone ID Related Accession (Same CDS sequence)	XM_010220031.1
Accession Version	XM_010220031.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-12
Organism	Tinamus guttatus(white-throated tinamou)
Product	paraplegin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010585302.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Tinamus guttatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGAAACTC AATTCCAGTT GTCTTCTCTT TCCTTCTTCT TTCTTTCTCT TCTTCTCCAG 
AGCCTTCTGT CGCAAACGCC TTCCCCCCCA TTTCAAGGTC TCAGTGGAGC CCTGGTGAAA 
CAACACTTGA TTCGAGATCC CGTCAGGCTT TGGAAGCTCT TAGGTGGCAC TTACTATTTC 
AGTACTTCAA GATCTCAAGG GAGTAACCAT AAGAATGGAG GATTCCAAAA GAAAACTCCA 
AAGGAGGATG AGGAGGATGA AAAACGCAGG AAACGAGAAA ATCAGATGCA TCTTGACCGC 
TTGCGGGCAC TCCTCGTCAT AACTTTCATA CTCCTGATGT TTCGATTCAC GGTCAGCGAG 
AACAGAGAAG GGACCAACAT TTCCTGGAAT TACTTTGTGA ATGAGATGCT GGCAAAAGGA 
GAGGTCCAGA GAGTTGAAGT AGTTCCGGAG AGTGATATTG TGGAAGTTTA TCTGCACCCA 
GGAGGAACTC CACATGGGCA AGTTAACGTT ACCCTGGTGT ACACGATGCG GGTGGCAAAC 
ATTGACAAGT TTGAGGAGAA GCTGAGAGCT GTGGAGGATG AGCTGAATAT CGATGAGAGA 
GAGAGACTCC CTGTTTCCTA CAAACACCCT GGCTTTTATG GAAATGACCT GTTCTCCCTG 
ATAGTGACCC TTGTGGCTTT GTCGATGTTG TGGAGCATCT TCCGCCTCGC TCGGGTGGCG 
AGCCGGGTGG GAGGATTCAA TGCCTTTAAC CAGCTGAAAA TGGCTCGTTT CACTGTCGTG 
GATGGCAAGT CTGGAAAAGG GATTGGCTTC AAGGATGTAG CAGGAAAGCA TGAGGCCAAA 
AGGGAGGTCA AAGAATTTGT GGATTATTTA AAGAGTCCGG AGCGCTACCT CCAGCTTGGG 
GCTAAAGTTC CCAAGGGCGC CCTGTTGCTC GGGCCGCCCG GCTGCGGGAA GACCTTGCTG 
GCGAAGGCGG TGGCTACCGA GGCGCAAGTG CCGTTCCTGG CCATGGCGGG CTCCGAGTTC 
GTGGAGGTGA TAGGAGGTCT CGGAGCTGCC CGAGTTCGGA GCCTCTTCAG AGAGGCGCAG 
GCCCGTGCGC CCTGCATCGT GTACATCGAC GAGATCGACG CCGTGGGCAA GAAGCGCTCC 
ACCAATATAT CTGGTTTTGC CAATGCCGAG GAGGAGCAGA CTTTAAACCA GCTGCTCGTG 
GAAATGGATG GAATGGGGAC CACGGATCAT GTCATCGTGC TGGCTTCCAC CAACCGCGCC 
GACGTCCTGG ATAACGCTCT GATGCGGCCC GGGCGGCTCG ACAGACACAT CTTCATTGAC 
CTTCCGACGC TGCAGGAGAG GAGAGAAATC TTCGAGCAGC ACCTGAAGAG TCTCAAGCTG 
ATCCAGGATG GCAGCTTCTA CTCGCAGCAC CTCGCTGAGC TGACTCCAGG CTTCAGTGGA 
GCTGACATAG CAAATATATG TAACGAAGCT GCTCTTCATG CTGCCAGAGA GGGTCACAAA 
TCCATTGATA CTTCCAACTT TGAGTATGCT GTGGAAAGAG TCATTGCAGG CACTGCCAAA 
AGGAGCAAGA TCCTGACACC AGAAGAGAGG AAGGTGGTGG CGTTCCACGA ATCGGGCCAC 
GCGCTGGTGG GCTGGCTGCT GGAGCACACG GAGGCTGTGA TGAAGGTTTC CATAGCCCCT 
CGAACAAATG CAGCTCTGGG ATTTGCTCAG ATCCTGCCAA GAGAGCAGTA CCTCTTCACC 
AAGGAGCAGC TGCTGGAGAG GATGTGTATG GCTCTCGGGG GCAGAGTGTC CGAGGCCATC 
ACCTTTAACA AGGTCACTAC AGGAGCACAG GATGACCTGA AGAAGGTGAC CCAGATCGCC 
TACTCCATGG TGAAGCAGTA CGGGATGGTG CCCAGCATCG GGCAGCTCTC CTTCCCGGAC 
GCGGAGAGCG CAGCCGGAAT TGGTCGGCGC CCTTTCAGCC AGGGACTTCA GCAGATGATG 
GACCATGAGG CAAAGGTGCT GGTGGCTCAG GCTTACAGAC GCACGGAGAA GCTGTTGTTG 
GACAACCGGG ACAAGCTGCA GGCGCTCTCT AATGCCCTCC TGGAGAAAGA AGTGATAAAT 
TACGATGACA TTGAAGCTTT GATTGGGCCT CCACCTTATG GGCCAAAGAA AATGATAGCT 
CCCCAGAGCT GGCTTCAAGC AGAGCGAGAC AAGCAAGACA CAAGAGACGA AGAGATGCCC 
CAGCAGCCAC CAAACCATGA GGAGGAGGAG GAACCGCGGC TGAGACCAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010218333.1
CDS	1..2274
Translation	METQFQLSSLSFFFLSLLLQSLLSQTPSPPFQGLSGALVKQHLIRDPVRLWKLLGGTYYFSTSRSQGSNHKNGGFQKKTPKEDEEDEKRRKRENQMHLDRLRALLVITFILLMFRFTVSENREGTNISWNYFVNEMLAKGEVQRVEVVPESDIVEVYLHPGGTPHGQVNVTLVYTMRVANIDKFEEKLRAVEDELNIDERERLPVSYKHPGFYGNDLFSLIVTLVALSMLWSIFRLARVASRVGGFNAFNQLKMARFTVVDGKSGKGIGFKDVAGKHEAKREVKEFVDYLKSPERYLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGSEFVEVIGGLGAARVRSLFREAQARAPCIVYIDEIDAVGKKRSTNISGFANAEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADVLDNALMRPGRLDRHIFIDLPTLQERREIFEQHLKSLKLIQDGSFYSQHLAELTPGFSGADIANICNEAALHAAREGHKSIDTSNFEYAVERVIAGTAKRSKILTPEERKVVAFHESGHALVGWLLEHTEAVMKVSIAPRTNAALGFAQILPREQYLFTKEQLLERMCMALGGRVSEAITFNKVTTGAQDDLKKVTQIAYSMVKQYGMVPSIGQLSFPDAESAAGIGRRPFSQGLQQMMDHEAKVLVAQAYRRTEKLLLDNRDKLQALSNALLEKEVINYDDIEALIGPPPYGPKKMIAPQSWLQAERDKQDTRDEEMPQQPPNHEEEEEPRLRPV

Target ORF information:

RefSeq Version	XM_010220031.1
Organism	Tinamus guttatus(white-throated tinamou)
Definition	Tinamus guttatus spastic paraplegia 7 (pure and complicated autosomal recessive) (SPG7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010220031.1

ORF Insert Sequence:

ATGGAAACTC AATTCCAGTT GTCTTCTCTT TCCTTCTTCT TTCTTTCTCT TCTTCTCCAG 
AGCCTTCTGT CGCAAACGCC TTCCCCCCCA TTTCAAGGTC TCAGTGGAGC CCTGGTGAAA 
CAACACTTGA TTCGAGATCC CGTCAGGCTT TGGAAGCTCT TAGGTGGCAC TTACTATTTC 
AGTACTTCAA GATCTCAAGG GAGTAACCAT AAGAATGGAG GATTCCAAAA GAAAACTCCA 
AAGGAGGATG AGGAGGATGA AAAACGCAGG AAACGAGAAA ATCAGATGCA TCTTGACCGC 
TTGCGGGCAC TCCTCGTCAT AACTTTCATA CTCCTGATGT TTCGATTCAC GGTCAGCGAG 
AACAGAGAAG GGACCAACAT TTCCTGGAAT TACTTTGTGA ATGAGATGCT GGCAAAAGGA 
GAGGTCCAGA GAGTTGAAGT AGTTCCGGAG AGTGATATTG TGGAAGTTTA TCTGCACCCA 
GGAGGAACTC CACATGGGCA AGTTAACGTT ACCCTGGTGT ACACGATGCG GGTGGCAAAC 
ATTGACAAGT TTGAGGAGAA GCTGAGAGCT GTGGAGGATG AGCTGAATAT CGATGAGAGA 
GAGAGACTCC CTGTTTCCTA CAAACACCCT GGCTTTTATG GAAATGACCT GTTCTCCCTG 
ATAGTGACCC TTGTGGCTTT GTCGATGTTG TGGAGCATCT TCCGCCTCGC TCGGGTGGCG 
AGCCGGGTGG GAGGATTCAA TGCCTTTAAC CAGCTGAAAA TGGCTCGTTT CACTGTCGTG 
GATGGCAAGT CTGGAAAAGG GATTGGCTTC AAGGATGTAG CAGGAAAGCA TGAGGCCAAA 
AGGGAGGTCA AAGAATTTGT GGATTATTTA AAGAGTCCGG AGCGCTACCT CCAGCTTGGG 
GCTAAAGTTC CCAAGGGCGC CCTGTTGCTC GGGCCGCCCG GCTGCGGGAA GACCTTGCTG 
GCGAAGGCGG TGGCTACCGA GGCGCAAGTG CCGTTCCTGG CCATGGCGGG CTCCGAGTTC 
GTGGAGGTGA TAGGAGGTCT CGGAGCTGCC CGAGTTCGGA GCCTCTTCAG AGAGGCGCAG 
GCCCGTGCGC CCTGCATCGT GTACATCGAC GAGATCGACG CCGTGGGCAA GAAGCGCTCC 
ACCAATATAT CTGGTTTTGC CAATGCCGAG GAGGAGCAGA CTTTAAACCA GCTGCTCGTG 
GAAATGGATG GAATGGGGAC CACGGATCAT GTCATCGTGC TGGCTTCCAC CAACCGCGCC 
GACGTCCTGG ATAACGCTCT GATGCGGCCC GGGCGGCTCG ACAGACACAT CTTCATTGAC 
CTTCCGACGC TGCAGGAGAG GAGAGAAATC TTCGAGCAGC ACCTGAAGAG TCTCAAGCTG 
ATCCAGGATG GCAGCTTCTA CTCGCAGCAC CTCGCTGAGC TGACTCCAGG CTTCAGTGGA 
GCTGACATAG CAAATATATG TAACGAAGCT GCTCTTCATG CTGCCAGAGA GGGTCACAAA 
TCCATTGATA CTTCCAACTT TGAGTATGCT GTGGAAAGAG TCATTGCAGG CACTGCCAAA 
AGGAGCAAGA TCCTGACACC AGAAGAGAGG AAGGTGGTGG CGTTCCACGA ATCGGGCCAC 
GCGCTGGTGG GCTGGCTGCT GGAGCACACG GAGGCTGTGA TGAAGGTTTC CATAGCCCCT 
CGAACAAATG CAGCTCTGGG ATTTGCTCAG ATCCTGCCAA GAGAGCAGTA CCTCTTCACC 
AAGGAGCAGC TGCTGGAGAG GATGTGTATG GCTCTCGGGG GCAGAGTGTC CGAGGCCATC 
ACCTTTAACA AGGTCACTAC AGGAGCACAG GATGACCTGA AGAAGGTGAC CCAGATCGCC 
TACTCCATGG TGAAGCAGTA CGGGATGGTG CCCAGCATCG GGCAGCTCTC CTTCCCGGAC 
GCGGAGAGCG CAGCCGGAAT TGGTCGGCGC CCTTTCAGCC AGGGACTTCA GCAGATGATG 
GACCATGAGG CAAAGGTGCT GGTGGCTCAG GCTTACAGAC GCACGGAGAA GCTGTTGTTG 
GACAACCGGG ACAAGCTGCA GGCGCTCTCT AATGCCCTCC TGGAGAAAGA AGTGATAAAT 
TACGATGACA TTGAAGCTTT GATTGGGCCT CCACCTTATG GGCCAAAGAA AATGATAGCT 
CCCCAGAGCT GGCTTCAAGC AGAGCGAGAC AAGCAAGACA CAAGAGACGA AGAGATGCCC 
CAGCAGCCAC CAAACCATGA GGAGGAGGAG GAACCGCGGC TGAGACCAGT ATGA