TTHERM_00859240 cDNA ORF clone, Tetrahymena thermophila SB210

Gene
Clones
gRNAs

Summary

Gene Symbol	TTHERM_00859240
Entrez Gene ID	7835374
Full Name	alpha/beta superfamily hydrolase
Gene Type	protein-coding
Organism	Tetrahymena thermophila SB210

mRNA and Protein(s)

mRNA	Protein	Name
XM_001021964.4	XP_001021964.3	alpha/beta superfamily hydrolase

Pathways from BioSystems
Gene Ontology

The following TTHERM_00859240 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTHERM_00859240 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OTe54324	XM_001021964.4 Latest version!	Tetrahymena thermophila SB210 alpha/beta superfamily hydrolase partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TTHERM_00859240 ( XM_001021964.4 ) cDNA ORF clone, Tetrahymena thermophila SB210 -> XP_001021964.3 Tetrahymena thermophila SB210 alpha/beta superfamily hydrolase partial mRNA.

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CloneID	OTe54324
Clone ID Related Accession (Same CDS sequence)	XM_001021964.4
Accession Version	XM_001021964.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-08
Organism	Tetrahymena thermophila SB210
Product	alpha/beta superfamily hydrolase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NW_002476271). On Jun 9, 2015 this sequence version replaced XM_001021964.3. COMPLETENESS: incomplete on the 3' end.

ATGTCTGTAG ACAGAATCAT TTTTCCTCGT CCAAAACCTC AATATAATGA GTAATCCATA 
AGAACCAAGG ATGAAAATTC GTCCATCTAA TTTATAGATT CTCCATCAGT AAAGAATCAA 
AATGAGATCC ACCGCGTTCC AGTGATGTCT ATGGTTTATA AAATATATGA AGAATCTTGT 
GATATTTATA TCTTATTTTT TCATGGTAAT GCTGAAGATT TGGGGAGCTC TATGCAGTTC 
TTAAAGCTTT TGAGAGAGAG CTTAAGAGCA AATATTATAG CTGTTGAATA TCCTGGTTAT 
GGTATATATG ACAAGAAGGT ATCTGCTGAA TAAATTAAGC AAGATGCTTT AAAGGTTTAT 
GATTCTTTAG TTGTAGATTC AGGTATTGAT CAGTCAAAGA TATTCGTGTT TGGTCGTTCT 
ATAGGAACTG GACCAGCTTG TGAAATAGGA GCTAGAAGAA GACCTGGAGG AGTAATCCTC 
TTAAGCGCTT TTACATCAAT AAAGAAGTTG TCAGGTGAGC TAGCTTTTAG CTTAGTAAGC 
TATTTTATAA AAGAGAGATT TAACAATATA GAAAATGTAT GTCGTTTCAG TTCTCCATGT 
CTACTTATCC ATGGTCAAGC AGACAGTCTT ATAAAACATT AACATTCTCA ATAACTCCAA 
GAGGCTATGA GATTAAATGG CAAGATAGTG CTTGCTTTTT ATCCTGAGAA GATGACTCAC 
AATCAATTTT AGATCAGAAA AGATATCATC AGACCTATCG ATACATTTTT AAAAAGAATT 
CAATTTCAAC AAGTTAAGAT TCAAACAAAG TTACTACCAG ATATATTTAA GAATTCTCTA 
TCTTAAGTAT AACTAATAAA TAAAAATTTC AGACAAGCAA CTGAAGAATA AAATAAATAA 
CTACCAAAAT AACAGCAGTA ATAACAATCC TAGTTACCTT TAAAACTATA AAAGTCAGAA 
TCATATAGCA GTAAAGAGCT TTAAAAAAAC AATAATCTTG GAATTTTAAG TGATAGAAAA 
CATAGATCTT AAGACTAATA GAGATAGATT AACAGTAATA ACTAGAACCA ATTAGAATAG 
AAAAAAGTTA TCAATAAAAA GAAACTAGAT CAACAACAAT AATAAGTGCT ACACATTGAA 
GTGTAATAAT ATGAAATTAC AGAGAAAAAA GAAGAAAGAA GTAATGCAAA TATCTACAAA 
CTTAAATATT CATAAAAATC ATAACAAGAT AAATATGTCA TTTAACCAAT TTAACCATAT 
CAAAGTAATA TACTGCAACC TTAATAATAA GAAAATCATA ATTTTTAGTT TATTAAATTA 
CAAAACCAAC TCAGAAATGT TGATTCATCT CCAGAATATT AAAAAGAAAA AATTCATTAA 
AACTAAAATC AGTAATATGA AGCCAAATAG AAAGAAAATC ATCATAAAAA CGGAGAAGAC 
GATGATATAA AAGATCATTA AAGTGATTTT ATGTATGACT AAATTAACAT ACTTGATGAG 
CCTCCTTTCG AATAAATTTT TAGTGAAAAT AATGTAAACA ACTTAGCAAA CAACCAGGAT 
TAAAATAAAT AAGAGCAAAA ACAAGAATAA GAATAGTAAG AACAACAGCA ACAAGAAGAG 
GAAGAGCCAT ATTATTAATA ACCCTATTGT GAAAATTATA CAAACGAAGA TTATTATTAA 
GTTTATGCTA CAAATGAAGA TTTCGAAAAC TAAGTTACTA CAATTAATTA AGCTTAATAG 
ATGCAAGTGA ATCATGCAAA TAATTTTTAT TTTAAAAATT AACATTAATA TAATGAGAAT 
ATTGAAACTA ATGAACACAT TTTTATGAAT AACAATAATT CAATTTTCTA AAAAATTGAT 
GATTTGGAAA TCTGTAATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001021964.3
CDS	142..2022
Translation	MSVDRIIFPRPKPQYNESIRTKDENSSIFIDSPSVKNQNEIHRVPVMSMVYKIYEESCDIYILFFHGNAEDLGSSMQFLKLLRESLRANIIAVEYPGYGIYDKKVSAEIKQDALKVYDSLVVDSGIDQSKIFVFGRSIGTGPACEIGARRRPGGVILLSAFTSIKKLSGELAFSLVSYFIKERFNNIENVCRFSSPCLLIHGQADSLIKHHSQLQEAMRLNGKIVLAFYPEKMTHNQFIRKDIIRPIDTFLKRIQFQQVKIQTKLLPDIFKNSLSVLINKNFRQATEENKLPKQQQSLPLKLKSESYSSKELKNNNLGILSDRKHRSDRINSNNNQLEKKVINKKKLDQQQVLHIEVYEITEKKEERSNANIYKLKYSKSQDKYVIPIPYQSNILQP**ENHNFFIKLQNQLRNVDSSPEYKEKIHNNQYEAKKENHHKNGEDDDIKDHSDFMYDINILDEPPFEIFSENNVNNLANNQDNKEQKQEEEQQQQEEEEPYYPYCENYTNEDYYVYATNEDFENVTTINA*MQVNHANNFYFKNHYNENIETNEHIFMNNNNSIFKIDDLEICN

Target ORF information:

RefSeq Version	XM_001021964.4
Organism	Tetrahymena thermophila SB210
Definition	Tetrahymena thermophila SB210 alpha/beta superfamily hydrolase partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001021964.4

ORF Insert Sequence:

ATGTCTGTAG ACAGAATCAT TTTTCCTCGT CCAAAACCTC AATATAATGA GTAATCCATA 
AGAACCAAGG ATGAAAATTC GTCCATCTAA TTTATAGATT CTCCATCAGT AAAGAATCAA 
AATGAGATCC ACCGCGTTCC AGTGATGTCT ATGGTTTATA AAATATATGA AGAATCTTGT 
GATATTTATA TCTTATTTTT TCATGGTAAT GCTGAAGATT TGGGGAGCTC TATGCAGTTC 
TTAAAGCTTT TGAGAGAGAG CTTAAGAGCA AATATTATAG CTGTTGAATA TCCTGGTTAT 
GGTATATATG ACAAGAAGGT ATCTGCTGAA TAAATTAAGC AAGATGCTTT AAAGGTTTAT 
GATTCTTTAG TTGTAGATTC AGGTATTGAT CAGTCAAAGA TATTCGTGTT TGGTCGTTCT 
ATAGGAACTG GACCAGCTTG TGAAATAGGA GCTAGAAGAA GACCTGGAGG AGTAATCCTC 
TTAAGCGCTT TTACATCAAT AAAGAAGTTG TCAGGTGAGC TAGCTTTTAG CTTAGTAAGC 
TATTTTATAA AAGAGAGATT TAACAATATA GAAAATGTAT GTCGTTTCAG TTCTCCATGT 
CTACTTATCC ATGGTCAAGC AGACAGTCTT ATAAAACATT AACATTCTCA ATAACTCCAA 
GAGGCTATGA GATTAAATGG CAAGATAGTG CTTGCTTTTT ATCCTGAGAA GATGACTCAC 
AATCAATTTT AGATCAGAAA AGATATCATC AGACCTATCG ATACATTTTT AAAAAGAATT 
CAATTTCAAC AAGTTAAGAT TCAAACAAAG TTACTACCAG ATATATTTAA GAATTCTCTA 
TCTTAAGTAT AACTAATAAA TAAAAATTTC AGACAAGCAA CTGAAGAATA AAATAAATAA 
CTACCAAAAT AACAGCAGTA ATAACAATCC TAGTTACCTT TAAAACTATA AAAGTCAGAA 
TCATATAGCA GTAAAGAGCT TTAAAAAAAC AATAATCTTG GAATTTTAAG TGATAGAAAA 
CATAGATCTT AAGACTAATA GAGATAGATT AACAGTAATA ACTAGAACCA ATTAGAATAG 
AAAAAAGTTA TCAATAAAAA GAAACTAGAT CAACAACAAT AATAAGTGCT ACACATTGAA 
GTGTAATAAT ATGAAATTAC AGAGAAAAAA GAAGAAAGAA GTAATGCAAA TATCTACAAA 
CTTAAATATT CATAAAAATC ATAACAAGAT AAATATGTCA TTTAACCAAT TTAACCATAT 
CAAAGTAATA TACTGCAACC TTAATAATAA GAAAATCATA ATTTTTAGTT TATTAAATTA 
CAAAACCAAC TCAGAAATGT TGATTCATCT CCAGAATATT AAAAAGAAAA AATTCATTAA 
AACTAAAATC AGTAATATGA AGCCAAATAG AAAGAAAATC ATCATAAAAA CGGAGAAGAC 
GATGATATAA AAGATCATTA AAGTGATTTT ATGTATGACT AAATTAACAT ACTTGATGAG 
CCTCCTTTCG AATAAATTTT TAGTGAAAAT AATGTAAACA ACTTAGCAAA CAACCAGGAT 
TAAAATAAAT AAGAGCAAAA ACAAGAATAA GAATAGTAAG AACAACAGCA ACAAGAAGAG 
GAAGAGCCAT ATTATTAATA ACCCTATTGT GAAAATTATA CAAACGAAGA TTATTATTAA 
GTTTATGCTA CAAATGAAGA TTTCGAAAAC TAAGTTACTA CAATTAATTA AGCTTAATAG 
ATGCAAGTGA ATCATGCAAA TAATTTTTAT TTTAAAAATT AACATTAATA TAATGAGAAT 
ATTGAAACTA ATGAACACAT TTTTATGAAT AACAATAATT CAATTTTCTA AAAAATTGAT 
GATTTGGAAA TCTGTAATTG A